45 results about "Phospholipases A" patented technology

Methods are provided for maintaining or lowering lipoprotein-associated phospholipase A₂ [“Lp-PLA₂”] levels, stabilizing rupture prone-atherosclerotic lesions, decreasing the Inflammatory Index and increasing Total Omega-3 Score™ in humans, by administering an effective amount of eicosapentaecnoic acid [“EPA”], an omega-3 polyunsaturated fatty acid [“PUFA”].

A method for degumming an oil composition comprises the steps of (a) providing an oil composition containing a quantity of phospholipids, (b) contacting said oil composition simultaneously with one or more phospholipase A enzymes and one or more phospholipase C enzymes, under conditions sufficient for the enzymes to react with the phospholipids to create phospholipid reaction products, and (c) separating the phospholipids reaction products from the oil composition, the remaining oil composition after the separation being a degummed oil composition, whereby during step (b) the reaction of said one or more phospholipase A enzymes proceeds at a faster rate than it would in the absence of said one or more phospholipase C enzymes.

The invention provides novel polypeptides having phospholipase activity, including, e.g., phospholipase A, B, C and D activity, patatin activity, lipid acyl hydrolase (LAH) activity, nucleic acids encoding them and antibodies that bind to them. Industrial methods, e.g., oil degumming, and products comprising use of these phospholipases are also provided.

Novel compounds are disclosed which inhibit the activity of phospholipase enzymes in a mammal, particularly cytosolic phospholipase A₂. Pharmaceutical compositions comprising such compounds and methods of treatment using such compositions are also disclosed.

This invention provides methods for the use of substituted indole compounds of the general formula:

and pharmaceutically acceptable salt forms thereof. The invention provides methods for the use of the compounds as inhibitors of the activity of various phospholipase enzymes, particularly phospholipase A₂ enzymes, and for the medical treatment, prevention and inhibition diseases and disorders including asthma, stroke, atherosclerosis, multiple sclerosis, Parkinson's disease, arthritic disorders, rheumatic disorders, central nervous system damage resulting from stroke, central nervous system damage resulting from ischemia, central nervous system damage resulting from trauma, inflammation caused or potentiated by prostaglandins, inflammation caused or potentiated by leukotrienes, inflammation caused or potentiated by platelet activation factor, pain caused or potentiated by prostaglandins, pain caused or potentiated by leukotrienes, and pain caused or potentiated by platelet activation factor.

The invention relates to phospholipase(s), isoforms, derivatives, mutants and/or fragments thereof, for the preparation of a medicament for the treatment and/or prevention of ischemia. Preferred is the use of secretory phospholipase, particularly phospholipase A₂, and even more particularly phospholipase A₂ derived from the snake venom of Naja sputatrix

The invention discloses a method for degumming fragrant rap oil through an enzymic process. The method is characterized by comprising the following steps: heating the squeezed fragrant crude rap oil without impurities to about 40 DEG C, adding a 50% citric acid aqueous solution according to 0.1% by weight of the oil, mixing the 50% citric acid aqueous solution with the fragrant crude rap oil, mixing and stirring at a rate of 50-60r/min, and stirring for one hour; adding soft water according to 1.8% by weight of the oil, adding a phospholipase A, phospholipase B or phospholipase C solution diluted to 100 times according to 0.13% of the total mass of oil and water, simultaneously stirring and reacting for 60 minutes at a rate of 40-50r/min under the temperature of 30-50 DEG C, filtering or centrifugally separating, and drying to obtain the finished product of the fragrant rap oil.

The invention provides a cellulose acetate/polypropylene composite film prepared by using cellulose acetate and polypropylene film as materials, adopts an adsorption-crosslinking immobilization method, and uses the composite film to immobilize phospholipase. The effects of different immobilization conditions such as adsorption time, enzyme solution concentration, crosslinking agent concentration, crosslinking time and crosslinking temperature on the immobilization efficiency and catalytic effect of phospholipase were studied, and the enzymatic properties of the immobilized enzyme membrane were investigated. discuss. The advantage of using cellulose acetate/polypropylene composite membrane to immobilize phospholipase was further explained by the SEM images of the immobilized enzyme membrane. According to the adsorption characteristics of the composite membrane in the present invention, the immobilized phospholipase improves the activity and stability of the enzyme in the reaction system, regulates and controls the activity and selectivity of the enzyme, thereby facilitating the recovery of the enzyme and the production of the product, and finally obtains The immobilized enzyme activity is 4.6U/cm2.

The invention provides a microbial oil preparation method. The microbial oil preparation method comprises the steps that fermentation is conducted to obtain oil-producing microbial fermentation liquor; the fermentation liquor is concentrated to obtain concentrated fermentation liquor with the solid content higher than 10%; the PH value of the fermentation liquor is regulated between 5 and 8.1, phospholipase A is added into the fermentation liquor, the temperature is controlled between 38 DEG C and 51 DEG C, and the fermentation liquor is stirred and sheared for 2-6 hours; the oil phase and the water phase are separated to obtain microbial oil with the phosphorus content lower than 5 ppm. The microbial oil preparation method has the advantages that the degumming process and the microorganism wall breaking process are combined, so that oil preparation processes are reduced, and production cost is lowered; furthermore, the degumming process is conducted before the crude oil preparation process, so that produced crude oil is higher in quality.

The present disclosure highlights the relationship between extracellular mitochondrial components, optionally in combination with the secreted phospholipase A₂-IIA and/or an auto-antibody, and in vivo as well as in vitro and inflammatory reactions/conditions, especially those released as a result of the degradation of a platelet. The present disclosure provides methods for determining the presence of inflammatory mediators, for limiting inflammatory reactions/conditions, for the diagnosis inflammatory reactions/conditions, for screening therapeutics for the treatment and/or the alleviation of symptoms of inflammatory reactions/conditions based on the detection or modulation of the level of these extracellular mitochondrial components.

The invention provides a compound enzyme, a highly-fragrant rapeseed oil processing method, highly-fragrant rapeseed oil and edible oil. Based on enzyme activity, the compound enzyme comprises 8092-8751 U/g of phospholipase A, 7317-7915 U/g of phospholipase C, 4154-4767 U/g of protease and 4047-4618 U/g of cellulase. The highly-fragrant rapeseed oil processing method comprises the following steps:mixing crude rapeseed oil with acid to be subjected to a first reaction to obtain an acid reaction liquid; mixing the acid reaction liquid with water and the compound enzyme to be subjected to a second reaction to obtain an enzyme reaction liquid; and carrying out inactivation treatment on the enzyme reaction liquid and then carrying out separation to obtain degummed oil. The highly-fragrant rapeseed oil is prepared by using the highly-fragrant rapeseed oil processing method. The edible oil comprises the highly-fragrant rapeseed oil. Through adoption of the compound enzyme and the highly-fragrant rapeseed oil processing method, the cost is low, the production efficiency is high, the yield is high, and the rapeseed oil has rich fragrance.

The invention relates to a novel use of a phospholipase A in the production of cake to improve at least one of the properties selected from the group consisting of: (i) batter viscosity, (ii) specific density, (iii) initial crumb softness, (iv) crumb pore homogeneity, (v) crumb pore diameter, (vi) crumb softness upon storage, (vii) shelf life and/or (viii) cake volume. The invention also relates to a novel use of phospholipase A in the production of cake to enable reduction of the amount of eggs and/or fat used in the recipe.

Phospholipo-proteins which have been enzyme modified may be used as an emulsifier to improve the emulsion stability of frozen products, e.g., during the freeze thaw and vacuum cool processes. Especially preferred is use of the enzyme phospholipase A to modify egg yolk and to use the modified egg yolk as an emulsifier in frozen products. Creation of a more stable emulsion can be expected to improve the overall appearance of the final product both in the frozen state and after the product has been reheated. Although egg yolk is preferred, examples of other phospholipo-protein containing substances include casein, skim milk, buttermilk, whey, cream, soybean, yeast, whole egg, blood serum and wheat proteins.