6 results about "Phospholipases C" patented technology

A medium for Bacillus cereus group detection, which is favorable in growth of Bacillus cereus regardless of the temperature condition, and further is excellent in selectivity; and a method for detecting a Bacillus cereus group using the medium. The medium for Bacillus cereus group detection includes a phosphatidylinositol-specific phospholipase C substrate having a detectable chromogenic or fluorescent free radical; and trimethoprim. The medium further includes a β-lactam antibiotic. The medium further includes an antifungal agent. The method for detecting further includes inoculating a sample into the medium to culture the sample; and determining a detectable colony on the medium.

Provided is a simple and specific means for detecting Listeria monocytogenes and / or Listeria ivanovii. A culture medium for detecting Listeria monocytogenes and / or Listeria ivanovii contains the following components (A), (B), and (C): (A) a chromogenic substance that develops color by being decomposed with β-glucosidase or a fluorogenic substance that emits fluorescence; (B) a chromogenic substance that develops color by being decomposed with phosphatidylinositol-specific phospholipase C or a fluorogenic substance that emits fluorescence; and (C) a sugar.

The application discloses a thermophilic phospholipase C mutant and a preparation method and application thereof. Based on a wild-type phospholipase TdPLC shown in SEQ ID NO. 1, a site-directed mutation is carried out, and a mutant T3A is formed by mutating threonine at the 3rd position. The mutant T3A has a significantly improved PA conversion rate, which is increased from 43.69% of the wild type to 73.54%, and has a good industrial potential. The application provides a theoretical basis for further improving the performance of phospholipase C such as TdPLC through protein engineering. The application also comprises nucleotides and construction, vectors and host cells for coding the mutant enzyme, as well as a method for producing the mutant and using the mutant.

The present disclosure relates to a synthesis method of β-nicotinamide mononucleotide (β-NMN) and an intermediate thereof. In the present disclosure, phospholipid metabolism enzymes phospholipase D (PLD) and phospholipase C (PLC) widely present in the biosphere are used as catalysts to prepare β-NMN through two-step enzymolysis or one-pot synthesis; and an intermediate, namely phosphatidyl nicotinamide riboside (PNR), is obtained during the two-step enzymolysis. The present disclosure has simple reaction steps, low production cost, and environmental friendliness, and is suitable for large-scale industrial production.

The application discloses a method for synergistically promoting enzymatic degumming of rapeseed oil by phospholipase C and phospholipase A2, and comprises the following steps: S1, acidizing crude rapeseed oil; S2, adjusting the pH value of the acidized rapeseed oil; S3, adding phospholipase C, phospholipase A2, calcium chloride and deionized water into the system after adjusting the pH value, mixing uniformly, and then performing an enzymatic degumming reaction under a preset reaction temperature by means of ultrasonic assistance; and S4, performing enzyme inactivation treatment and centrifugal separation to obtain degummed rapeseed oil. The method adopts a phospholipase C and phospholipase A2 complex enzyme system, utilizes the catalytic complementarity of the two enzymes to different phospholipid components, breaks through the bottleneck that single enzyme cannot completely degum high-phosphorus rapeseed oil, and combines an ultrasonic auxiliary technology, so that the mass transfer efficiency of the enzyme and the substrate is strengthened through an ultrasonic cavitation effect, the degumming time is shortened, an efficient, economical and green new degumming solution is provided for the rapeseed oil refining industry, and the method has significant technical innovation and industrial application value.

The invention provides a method for preparing diglyceride, which comprises the following steps: S1, carrying out first hydrolysis treatment on high-phosphorus-content plant crude oil by using phospholipase C to obtain a hydrolysis product; and S2, carrying out second hydrolysis treatment on the hydrolysis product by using lipase to obtain the diglyceride. According to the method, dephosphorization pretreatment is not needed, the diglyceride preparation process can be effectively simplified, the diglyceride yield is increased, meanwhile, the reaction process is precisely regulated and controlled to control the content of diglyceride in the product, the generation amount of free fatty acid is reduced, the product quality and the production efficiency are improved, and the method is suitable for industrial large-scale application.