14 results about "Phospholipases C" patented technology

The invention discloses a method for preparing uric acid-reducing diglyceride through enzymolysis and a product and application thereof, and belongs to the technical field of diglyceride production, the method comprises the following steps: (1) mixing vegetable oil, glycerol, water and an enzyme preparation for reaction to obtain a reaction solution; (2) standing and layering the reaction liquid, and collecting supernatant; (3) performing distillation separation on the supernatant, and performing decoloration and deodorization to obtain diglyceride; the enzyme preparation in the step (1) is prepared from lipase Lipase, esterase Esterase and phospholipase C in a weight ratio of (5-10): (0.1-0.3): 1. Compared with the prior art, the method disclosed by the invention is simple and low in cost, and the prepared diglyceride has more advantages in the aspect of reducing uric acid.

The present invention discloses a method for preparing uric acid-lowering diglyceride by enzymatic hydrolysis, as well as its product and application, belonging to the technical field of diglyceride production. The method comprises the following steps: (1) mixing vegetable oil, glycerol, water, and an enzyme preparation to react to obtain a reaction solution; (2) allowing the reaction solution to stand for stratification and collecting the supernatant; (3) distilling and separating the supernatant, decolorizing, and deodorizing the supernatant to obtain diglyceride; the enzyme preparation in step (1) comprises lipase, esterase, and phospholipase C in a weight ratio of 5-10:0.1-0.3:1. Compared with the existing technology, the method of the present invention is simple and low-cost, and the prepared diglyceride has greater advantages in lowering uric acid.

A medium for Bacillus cereus group detection, which is favorable in growth of Bacillus cereus regardless of the temperature condition, and further is excellent in selectivity; and a method for detecting a Bacillus cereus group using the medium. The medium for Bacillus cereus group detection includes a phosphatidylinositol-specific phospholipase C substrate having a detectable chromogenic or fluorescent free radical; and trimethoprim. The medium further includes a β-lactam antibiotic. The medium further includes an antifungal agent. The method for detecting further includes inoculating a sample into the medium to culture the sample; and determining a detectable colony on the medium.

The invention discloses an extraction method and application of natural limonene. The preparation method comprises the following steps: stripping fresh orange peel, treating with liquid nitrogen overnight, crushing, putting into a deep eutectic solvent (DES) composed of choline chloride and glycerol, and carrying out mild stirring extraction under the assistance of phospholipase C; as limonene is easy to oxidize and degrade in high-temperature, illumination and oxygen-enriched environments, the method adopts an enzyme extraction process which is mild in condition, efficient and environment-friendly, can effectively maintain the chemical stability and extraction quality of limonene, and has the advantages of high extraction rate, short time consumption, simplicity and convenience in operation and the like.

Provided is a simple and specific means for detecting Listeria monocytogenes and / or Listeria ivanovii. A culture medium for detecting Listeria monocytogenes and / or Listeria ivanovii contains the following components (A), (B), and (C): (A) a chromogenic substance that develops color by being decomposed with β-glucosidase or a fluorogenic substance that emits fluorescence; (B) a chromogenic substance that develops color by being decomposed with phosphatidylinositol-specific phospholipase C or a fluorogenic substance that emits fluorescence; and (C) a sugar.

The present disclosure generally relates to methods of treating diseases or disorders associated with dysfunctional phosphatidylinositol-specific phospholipase Cγ2 (PLCγ2), such as cancer and immune system disorders, using Bruton's tyrosine kinase (BTK) reducing molecules, such as BTK degrader molecules.

The application discloses a thermophilic phospholipase C mutant and a preparation method and application thereof. Based on a wild-type phospholipase TdPLC shown in SEQ ID NO. 1, a site-directed mutation is carried out, and a mutant T3A is formed by mutating threonine at the 3rd position. The mutant T3A has a significantly improved PA conversion rate, which is increased from 43.69% of the wild type to 73.54%, and has a good industrial potential. The application provides a theoretical basis for further improving the performance of phospholipase C such as TdPLC through protein engineering. The application also comprises nucleotides and construction, vectors and host cells for coding the mutant enzyme, as well as a method for producing the mutant and using the mutant.

The present disclosure is generally directed to methods of treating a disease or disorder associated with dysfunctional phosphatidylinositol-specific phospholipase Cγ2 (PLCγ2), such as cancers and immune system disorders, using Bruton's tyrosine kinase (BTK) reducing molecules, such as BTK degrader molecules.

The present disclosure relates to a synthesis method of β-nicotinamide mononucleotide (β-NMN) and an intermediate thereof. In the present disclosure, phospholipid metabolism enzymes phospholipase D (PLD) and phospholipase C (PLC) widely present in the biosphere are used as catalysts to prepare β-NMN through two-step enzymolysis or one-pot synthesis; and an intermediate, namely phosphatidyl nicotinamide riboside (PNR), is obtained during the two-step enzymolysis. The present disclosure has simple reaction steps, low production cost, and environmental friendliness, and is suitable for large-scale industrial production.

Bacterial cultures are provided that comprise a modified Pseudomonas aeruginosa bacterium missing or deficient in two or more virulence factors. The two or more virulence factors can be selected from exotoxin A, hemolytic phospholipase C, phenazine-specific methyltransferase, alpha-1,3-rhamnosyltransferase, and 3-phosphoshikimate 1-carboxyvinyltransferase. Certain of the modified Pseudomonas aeruginosa bacteria are also missing or deficient in one or more alginate acetylation enzymes including the alginate O-acetyltransferases AlgI, AlgJ, AlgF, AlgX, and / or the C5-mannuronan epimerase AlgG. Methods of producing alginate are also provided along with compositions comprising alginate produced by the modified Pseudomonas aeruginosa bacteria.

The present disclosure relates generally to methods of treating diseases or disorders associated with dysfunctional phosphatidylinositol-specific phospholipase C [gamma] 2 (PLC [gamma] 2), such as cancer and immune system disorders, using Bruton's tyrosine kinase (BTK) reducing molecules, such as BTK degrading agent molecules.

This application discloses a biological factor and its use in a drug for protecting auditory hair cells from damage, belonging to the field of biological inner ear research. The biological factor is a phospholipase C (PLC) family subtype PLCβ3, which has the effect of protecting auditory hair cells from damage by noise, platinum-based chemotherapy drugs, and aminoglycoside antibiotics. The amino acid sequence of the biological factor PLCβ3 in humans, mice, and zebrafish is shown as SEQ NOs.1, 2, and 3, and the nucleotide sequence in humans, mice, and zebrafish is shown as SEQ NOs.4, 5, and 6. This is the first time that the biological factor PLCβ3 has been discovered in the inner ear to have a protective effect on hair cells. This invention lays an important foundation for subsequent research on hair cell pre-protection and hair cell regeneration in mammals.

The application discloses a method for synergistically promoting enzymatic degumming of rapeseed oil by phospholipase C and phospholipase A2, and comprises the following steps: S1, acidizing crude rapeseed oil; S2, adjusting the pH value of the acidized rapeseed oil; S3, adding phospholipase C, phospholipase A2, calcium chloride and deionized water into the system after adjusting the pH value, mixing uniformly, and then performing an enzymatic degumming reaction under a preset reaction temperature by means of ultrasonic assistance; and S4, performing enzyme inactivation treatment and centrifugal separation to obtain degummed rapeseed oil. The method adopts a phospholipase C and phospholipase A2 complex enzyme system, utilizes the catalytic complementarity of the two enzymes to different phospholipid components, breaks through the bottleneck that single enzyme cannot completely degum high-phosphorus rapeseed oil, and combines an ultrasonic auxiliary technology, so that the mass transfer efficiency of the enzyme and the substrate is strengthened through an ultrasonic cavitation effect, the degumming time is shortened, an efficient, economical and green new degumming solution is provided for the rapeseed oil refining industry, and the method has significant technical innovation and industrial application value.

The invention provides a method for preparing diglyceride, which comprises the following steps: S1, carrying out first hydrolysis treatment on high-phosphorus-content plant crude oil by using phospholipase C to obtain a hydrolysis product; and S2, carrying out second hydrolysis treatment on the hydrolysis product by using lipase to obtain the diglyceride. According to the method, dephosphorization pretreatment is not needed, the diglyceride preparation process can be effectively simplified, the diglyceride yield is increased, meanwhile, the reaction process is precisely regulated and controlled to control the content of diglyceride in the product, the generation amount of free fatty acid is reduced, the product quality and the production efficiency are improved, and the method is suitable for industrial large-scale application.