Method for degumming soybean oil by phospholipase catalysis

A degumming method and phospholipase technology, applied in the field of vegetable oil degumming, can solve the problems of low gum removal rate, inability to be popularized on a large scale, and long time consumption, and achieve the effect of improving economic benefits

Inactive Publication Date: 2012-08-15
JIANGNAN UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

Degumming is mainly to remove phospholipids. If degumming is not complete, it will affect the deep processing of oil products and the stability of oil products, and reduce the shelf life of oil products.
Enzymatic degumming can overcome the problems of low gum removal rate and high loss of neutral oil in traditional degumming methods
The German Lurgi company was the first to introduce phospholipase A 2 Applied to the degumming of fats and oils, the enzyme is derived from the pancreas of pigs, the quantity is limited, the price is expensive, and calcium ions are used as the activator when it is used, so it cannot be popularized on a large scale
Novozymes subsequently launched two commercial phospholipase A products, Lecitase Ultra and Lecitase Novo. 1 However, the degumming reaction catalyzed by these two enzymes takes 4-5 hours, which is too long, and because the phospholipids are degummed by phospholipase A 1 Hydrolyzed into lysophospholipid and fatty acid salt, easy to emulsify and cause loss of neutral oil

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  • Method for degumming soybean oil by phospholipase catalysis

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specific Embodiment approach 1

[0010] Specific embodiment 1: Heat soybean oil to 70°C, add a citric acid solution with a mass concentration of 45% and a mass of 0.5% soybean oil, and carry out acid pretreatment for 20 minutes under stirring conditions of 500r / min; after acid treatment Cool the oil-water mixture to 47℃, add 4% NaOH solution by mass concentration, adjust the pH to 5, add 2% distilled water by oil weight, add phospholipase C at the ratio of 1000U / g oil, and mix well; set the stirring speed at Enzyme reaction is carried out at 300r / min, and the time is controlled at 1h; the reaction system is heated to above 90℃ to inactivate the enzyme for at least 10min, and centrifuged at above 8000r / min for at least 10min, to complete the phospholipase C catalyzed soybean oil.

[0011] After degumming in this embodiment, the phosphorus content of the degummed soybean oil is 23 mg / kg, the phosphorus content is reduced by about 87% compared to before degumming, and the glycolic acid content in soybean oil is incr...

specific Embodiment approach 2

[0012] Specific embodiment two: heating the hydrated degummed soybean oil to 80°C, adding a citric acid solution with a mass concentration of 50% and a mass of 0.5% soybean oil, and performing acid pretreatment for 20 minutes under stirring conditions of 500r / min; Cool the acid-treated oil-water mixture to 50°C, add 4% NaOH solution by mass concentration to adjust the pH to 5.2, add 4% oil weight distilled water, add phospholipase C according to the ratio of 1200U / g oil, mix well; The enzyme reaction is carried out at a speed of 400r / min and the time is controlled at 2h; the reaction system is heated to above 90°C to inactivate the enzyme for at least 10 minutes, and centrifuged above 8000r / min for at least 10 minutes to complete the degumming process of phospholipase C catalyzed soybean oil.

[0013] After degumming in this embodiment, the phosphorus content of the degummed soybean oil is 20 mg / kg, the phosphorus content is about 64% lower than that before degumming, and the glyc...

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Abstract

The invention discloses a method for degumming soybean oil by phospholipase catalysis, which belongs to the field of vegetable oil degumming and includes the steps of preheating, adjusting pH, adding enzyme prior to mixing, allowing for enzymolysis, deactivating the enzyme and separating. The method is technically characterized by including: using phospholipase C as the phospholipase for catalysis, and preheating crude soybean oil prior to acid treatment; cooling oil-water mixture subjected to acid treatment to 37-60 DEG C, adding NaOH solution to adjust pH to 3-8, adding distilled water accounting for 1%-5% of the weight of the oil, adding the phospholipase C according to an oil proportion of 100-2000U/g, and mixing well; setting the mixing speed at 100-600r/min, and allowing for enzyme reaction for 0.5-2 hours; and deactivating the enzyme at high temperature for a reaction system, and performing high-speed centrifugal separation to complete degumming. The degumming time of the method is shortened by 1-2 times as compared with that of using phospholipase A1, phosphorus content is reduced, diglyceride content of the oil is increased slightly, and economic benefit is increased for oil production industries.

Description

Technical field [0001] The invention is a soybean oil degumming method catalyzed by phospholipase C, and belongs to the field of vegetable oil degumming. Background technique: [0002] Degumming is an important part of the soybean oil refining process, and it is essential to improve the quality of the oil. Degumming is mainly to remove phospholipids. If degumming is not thorough, it will affect the deep processing of oil products and the stability of oil products, and reduce the shelf life of oil products. Enzymatic degumming can overcome the problems of traditional degumming methods such as low gel removal rate and high loss of neutral oil. The German Lurgi company first introduced phospholipase A 2 Applied to the degumming of fats, the enzyme is derived from the pancreas of pigs, is limited in quantity and expensive, requires calcium ions as an activator when used, and cannot be widely promoted. Subsequently, Novozymes launched two commercial phospholipase A, Lecitase Ultra a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B3/02A23D9/04
Inventor 金青哲杨娇王兴国
Owner JIANGNAN UNIV
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