193 results about "Diglyceride" patented technology

The invention relates to a diacylglycerol acyltransferase capable of synthesizing triglyceride as well as a coding gene and application of the diacylglycerol acyltransferase, and belongs to the field of enzymology, the nucleotide sequence of the gene for coding the diacylglycerol acyltransferase is as shown in SEQ ID NO.1, and the amino acid sequence of the diacylglycerol acyltransferase is as shown in SEQ ID NO.2. The invention also provides a recombinant plasmid and a recombinant engineering bacterium containing the gene of the enzyme. Diglyceride and an acyl donor are used as substrates, and triglyceride is catalytically synthesized through an acyl transfer reaction by using the enzyme. The invention provides an efficient tool for realizing enzymatic biosynthesis and large-scale production of natural triglyceride.

The invention discloses diglyceride prepared by an enzymic method as well as a preparation method and application thereof, and belongs to the technical field of deep processing of grease. The method comprises the following steps: adding fatty acid into a choline chloride-glycerol-urea ternary eutectic solvent, uniformly mixing, and then adding candida antarctica lipase B for esterification reaction to obtain a reaction mixture; separating the reaction mixture, and recovering an upper-layer oil phase; and separating and purifying the upper-layer oil phase to obtain the diglyceride. According to the method, the reaction rate is high, the selectivity of the diglyceride in the esterification process is high, the yield of the diglyceride can reach 65% or above by reacting for 1 h, the selectivity of the diglyceride is 75% or above, and the purity of the separated and purified diglyceride is 90% or above; in addition, the method is simple to operate, and the reaction product and the eutectic solvent can be separated through simple centrifugation after the reaction is finished; in addition, the monoglyceride content in the product is low, and the subsequent separation and purification difficulty is greatly reduced.

This invention discloses a method for preparing a diglyceride nanostructured lipid carrier hydrogel, comprising the following steps: mixing vegetable oil and diglyceride at a mass ratio of 1:1-3 as phase A; using an aqueous solution of soybean lecithin, tea saponin, and glycerol as phase B; adding phase B to phase A, followed by emulsification, ultrasonic treatment, and finally cooling to room temperature to obtain a diglyceride nanostructured lipid carrier; adding the diglyceride nanostructured lipid carrier to the hydrogel and mixing to obtain the diglyceride nanostructured lipid carrier hydrogel. This invention also discloses the application of the above-mentioned diglyceride nanostructured lipid carrier hydrogel. The diglyceride nanostructured lipid carrier hydrogel of this invention not only has good sun protection, antioxidant, and anti-glycation properties, but can also encapsulate unstable cosmetic raw materials.

An oral pouch product includes a wrapper and a filling material. The wrapper defines a cavity. The filling material is in the cavity. The filling material includes a dry mixture and a liquid mixture. The dry mixture includes a cellulosic material and a water-soluble filler. The liquid mixture includes an oil and liquid nicotine. The oil includes a triglyceride, a diglyceride, a monoglyceride, or any combination thereof. The liquid nicotine is dissolved in the oil. The filling material is free of water or includes water in an amount less than or equal to 5 weight percent.

The invention relates to the technical field of spectral analysis, and discloses a spectral analysis-based safflower oil diglyceride oil quality detection method. The method comprises the following steps: firstly, acquiring sample spectrum acquisition parameters and original spectrum data, wherein the data contains characteristic absorption peak wave band information and is acquired according to a preset scanning mode; preprocessing the original spectral data, and extracting feature absorption difference points to generate a feature mapping set; dividing an effective detection interval based on the feature mapping set, and adjusting spectrum acquisition parameters to form a correction parameter group; aligning the adjusted spectral data to generate multi-dimensional spectral data; and inputting an absorption peak matching model to carry out characteristic absorption peak matching processing, identifying a characteristic identifier and a quality abnormality identifier, and generating a quality judgment result. After a result is generated, the equipment working state and environmental parameters are monitored in real time, and the correction parameter set is dynamically adjusted to adapt to environmental changes. According to the method, the quality of the safflower oil diglyceride oil can be accurately detected, and the detection efficiency and reliability are improved.

This invention relates to the field of oil and fat synthesis technology, specifically to a method for preparing nervonic acid monoglyceride. In this invention, nervonic acid and glycerol are used as raw materials, and an esterification reaction is carried out under microwave assistance to obtain a first mixture containing nervonic acid monoglyceride, nervonic acid diglyceride, nervonic acid triglyceride, unreacted nervonic acid and glycerol, and a catalyst. Glycerol and the catalyst are removed by ethyl acetate extraction to obtain a second mixture. Subsequently, taking advantage of the temperature-sensitive nature of nervonic acid monoglyceride in edible oils, it is separated and purified to obtain nervonic acid monoglyceride. The esterification reaction under microwave assistance not only avoids the corrosion and contamination problems caused by traditional acid catalysts, but also significantly shortens the esterification reaction time, improves synthesis efficiency, and effectively inhibits the isomerization of cis-nervonic acid during the reaction, ensuring the stability of the product configuration.

This invention relates to the field of nutritional food technology, specifically to a fat-reducing composition for regulating the gut and its preparation method. The fat-reducing composition for regulating the gut comprises the following components by weight percentage: 20-25% solid coconut diglyceride microcrystalline powder, 15-20% fermented gel freeze-dried powder, 3-5% citrus pectin, 8-12% psyllium husk powder, 10-15% defatted coconut powder, 5-8% resistant dextrin, 8-10% pea protein powder, 3-5% freeze-dried mango powder, 4-7% erythritol, 1-2% microcrystalline cellulose, and 0.5-1% citric acid powder. This invention effectively solves the core problems of traditional fat-reducing products—poor ingredient stability, low bioavailability, and limited efficacy—through the dual progressive effects of physical structure and biological fermentation. It systematically achieves a synergistic effect of delaying fat digestion and absorption, long-lasting satiety enhancement, and precise regulation of gut microbiota health, completing a systematic engineering leap from single-component stimulation to simulating human metabolic pathways. This provides a stable, efficient, and safe complete solution for fat-reducing functional foods that regulate the gut.

The invention discloses diglyceride composite edible oil assisting in reducing blood sugar and blood fat and a preparation method thereof, and relates to the technical field of functional edible oil. The diglyceride composite edible oil for assisting in reducing blood sugar and blood fat is prepared from the following raw materials in parts by weight: 40 to 60 parts of high-purity diglyceride oil, 2 to 3 parts of modified flaxseed oil micro powder, 2 to 3 parts of modified camellia oleosa seed oil microcapsule powder, 5 to 8 parts of perilla seed oil, 2 to 5 parts of phytosterol ester, 1 to 3 parts of resistant dextrin, 0.1 to 0.5 part of rosemary extract and 0.05 to 0.15 part of potassium citrate. The compound edible oil is scientific in formula, and the high-purity diglyceride oil is used as a core and matched with various functional components such as the modified flaxseed oil micro powder to synergistically play a role in assisting in reducing blood sugar and blood fat. Through an exclusive modification process, gradient high-pressure homogenization and other standardized preparation processes, the stability and dispersion uniformity of the components are improved, the absorption rate of the active components is high, the storage life is long, no artificially synthesized additive is added, the food is safe to eat, and practicability and health are both considered.

This invention discloses a method for preparing ethylhexylglycerol and its application, comprising the following steps: adding isooctanol and a base to a solvent, stirring and cooling to 0-5 degrees Celsius, then adding a dichloromethane solution of p-toluenesulfonyl chloride dropwise. After the addition is complete, the mixture is brought to room temperature and stirred for a period of time. A sample is taken for gas chromatography analysis until the isooctanol is completely consumed. Water is added to separate the layers, and the dichloromethane layer is collected. The dichloromethane layer is washed twice with water and dried to obtain a dichloromethane solution of isooctyl p-toluenesulfonate, which is then dried and reserved. Glycerol and boric acid are added to a reaction flask for dehydration reaction, and after the reaction is complete, a diglyceride borate intermediate is obtained. The prepared diglyceride borate intermediate is added dropwise to a dichloromethane solution of isooctyl p-toluenesulfonate to obtain isooctyl borate diglyceride ether. Then, the solvent is removed, and the mixture is hydrolyzed with water to obtain the target compound, ethylhexylglycerol. This invention, through the application of the diglyceride borate structure, reduces the generation of by-products, and the overall yield is higher compared with current traditional processes.

The application belongs to the technical field of oil and fat preparation, and particularly relates to a preparation method of a glyceride oil with weight loss effect and application thereof. The method comprises the following steps: first, adding oil and fat into a basic nutrient solution, adding a bacterial strain for fermentation, filtering, and obtaining a fermentation product; then, adding glycerol into the fermentation product, adopting a lipase esterification reaction, and centrifugally separating to obtain an oil phase. The method adopts a dominant bacterial strain to degrade triglyceride, then adds glycerol, and adopts a dominant compound lipase to perform an esterification reaction, so as to obtain glyceride oil. The method can selectively generate 1,3-glyceride, and improve the proportion of the dominant oil and fat. Research shows that the glyceride oil prepared by the method has good weight loss and fat reduction effects, and has a good application prospect.

The invention relates to the non-therapeutic nutricosmetic use of a composition in a form suitable for oral administration comprising a wheat extract in a physiologically acceptable medium. The invention is characterized in that the wheat extract contains 35-40% of triglycerides and diglycerides, 20-30% of glycolipids, 7-10% of phospholipids and 2-4% of ceramides and glycosylceramides, by weight relative to the total weight of the extract, and is used to reduce excessive sebum secretion in the skin of the face.

This application relates to bioabsorbable cationic steroidal antimicrobial (CSA) compounds or salts thereof having a structure of formula I, II or III: formula (I), formula (II) or formula (III), wherein R1-R 18 At least one of them (e.g., R) 18 This includes monoglycerides or diglycerides of fatty acids linked by ester bonds, and R1-R 18 At least one of them (e.g., R3, R7, and R) 12 ) is an amino acid linked to the steroid backbone via an ester bond or an amide bond, wherein: R 18 It has the following structure: -R 19 -(C=O)-O-C-CR 20 -CR 21 , where R 19 The radical is omitted or selected from alkyl, alkenyl, ynyl, and aryl, and R 20 and R 21 Independently selected from hydroxyl, alkylcarboxyl, and aminoalkylcarboxyl groups, provided that R 20 and R 21 At least one of them is an alkyl carboxyl group, R3, R7 and R 12 It has the following structure: R 24 R 23 N-R 22 -(C=O)-X-, where R 22 It is a substituted or unsubstituted alkyl group, X is oxygen or nitrogen, and R is... 23 and R 24 It is independently hydrogen, alkyl, alkenyl, alkynyl, or aryl. (I)(II)(III)

The invention discloses an engineering bacterium for expressing camellia oleosa seed oil preference lipase and application of the engineering bacterium. Relates to the technical field of molecular biology and microorganisms. Preservation information, applications, and methods of preparation are provided. The efficient lipase is prepared through heterologous expression, and an innovative solution is provided for solving the key technical problems in camellia oleosa seed oil modification. The lipase expressed by the engineering bacteria provided by the invention can significantly reduce the content of triglyceride in camellia oleosa seed oil and increase the content of diglyceride, and can be applied to oil modification of camellia oleosa seed oil and increase the nutritional value.

Botanical extracts are provided, which comprise ethanol extracts of Nannochloropsis algae having between 10wt% and 50wt% polar lipids and between 45wt% and 60wt% fatty acids, wherein the fatty acids comprise more than 10wt% of eicosapentaenoic acid (EPA) as triglyceride conjugates and / or diglyceride conjugates, and less than 15wt% EPA as free fatty acids. EPA level may reach up to 90wt%, and the botanical extract may be fluid at room temperature. Growth conditions are configured, adjusted and monitored to promote EPA formation in the cell vacuoles, resulting in a high proportion of EPA as tri / di-glyceride conjugates, as well as in a high concentration of Ω-3 fatty acids, which merely requires gentle extraction procedures to reach the final products, thereby maintaining the biochemical of the compounds without excessive modifications.

The invention relates to a functional nutritional composition, in particular to an anti-fatigue diglyceride composition, which is characterized by comprising (1) diglyceride, (2) isomaltooligosacharide, (3) sodium caseinate, (4) soybean phospholipid, (5) sodium citrate, (6) potassium citrate, (7) calcium ascorbate, (8) silicon dioxide, (9) mogroside, (10) vitamin B1 and (11) vitamin B2. The composition disclosed by the invention can be used as an anti-fatigue beverage, a nutritional health-care product, a food for special diets, a food for special medical purposes and the like.

The invention discloses a method for increasing the content of polyphenols in diglyceride oil, particularly relates to the technical field of materials, and relates to a method for increasing the content of polyphenols in diglyceride oil. The diglyceride oil is prepared from activated polyphenol powder, vegetable oil, lipase and deionized water; the diglyceride oil is prepared from the following raw material components in parts by weight: 80 to 150 parts of activated polyphenol powder, 10000 parts of vegetable oil, 3 to 8 parts of lipase and 400 to 700 parts of deionized water. According to the invention, a synergistic effect mechanism of a compound emulsifier and enzyme catalysis is established, a molecular film is formed on an oil-water interface by utilizing a phospholipid and sucrose ester compound system, and meanwhile, molecular combination of polyphenol and glyceride is catalyzed by virtue of lipase to form a stable polyphenol-grease complex, so that migration and precipitation of polyphenol in a processing process are effectively prevented, and the stability of the polyphenol-grease complex is improved. Therefore, the polyphenol retention rate is greatly improved.

The application provides a white-borneol apricot kernel face mask liquid and a preparation method and application thereof, and relates to the technical field of cosmetics. The preparation raw materials of the white-borneol apricot kernel face mask liquid provided by the application include white petrolatum 5-20 parts, mono-diglyceride 1-10 parts, methyl p-hydroxybenzoate 0.01-8 parts, glycerol 1-10 parts, butanediol 0.05-10 parts, Tween-80 0.05-12 parts and compound extraction liquid 10-30 parts in terms of weight percentage. The white-borneol apricot kernel face mask liquid provided by the application has good moisturizing effect, is cold and heat resistant, has good stability and meets the microbial detection standard of cosmetics.

The application provides a method, application and product for preparing a triglyceride oil with five effects of improving blood vessel health by enzymolysis, and belongs to the technical field of enzyme engineering. The preparation method comprises the following steps: mixing low erucic acid rapeseed oil, glycerol, water, fatty acid and lipase, performing enzymolysis reaction, performing molecular distillation, and performing decolorization and deodorization to obtain the triglyceride oil; the lipase is a mixture of Candida antarctica black Aspergillus niger lipase, Candida cylindracea lipase and bovine pancreas lipase; and the weight ratio of the low erucic acid rapeseed oil, glycerol, water, fatty acid and lipase is 500:80-100:1-2:30-60:10-20. The triglyceride oil prepared by the method can be used to prepare a triglyceride oil composition, and has the five effects of lowering blood uric acid, lowering blood total cholesterol, lowering blood triglyceride, lowering blood sugar and improving blood flow speed, thereby improving blood vessel health.

The application discloses an enzymatic production process of diglyceride, and comprises the following steps: using low-cost lipase as a catalyst, preparing lipase A through immobilization of the enzyme, preparing lipase B through an ester exchange reaction, and then using the lipase B as a catalyst to catalyze esterification of free fatty acid and glycerol to produce diglyceride; the method can realize efficient enzymatic production of diglyceride through a series of methods by using low-cost lipase, and the production cost is reduced. The application provides an economical and efficient method for producing diglyceride by using the enzyme method, greatly reduces the use cost of lipase in the enzymatic esterification reaction, and has certain industrialization application prospect.

The utility model discloses a reaction kettle for diglyceride production with strong sealing performance, which comprises a bottom plate, a kettle body is arranged above the bottom plate, a kettle cover is arranged above the kettle body, the bottom of the kettle cover is fixedly connected with a rubber plate, and the rubber plate is attached to the kettle body. The kettle cover is correctly mounted by an operator, then the operator starts the first motor to rotate to drive the gear to rotate, the gear ring is further driven to rotate, the gear ring rotates to drive the supporting frame to rotate, the limiting rod and the locking block are further driven to move, and when the locking block is attached to the locking groove, the locking block can apply downward component force to the inner wall of the locking groove, so that the kettle cover is locked. When the rubber plate is pressed, the fixing frame and the kettle cover are subjected to downward force, the kettle cover presses the rubber plate, an operator can unwind the traction belt, then under the action of the torsional spring, the pressing frame rotates anticlockwise until the pressing frame presses the kettle cover and further presses the rubber plate, and the device has the advantage of being good in sealing effect.

The application discloses a bacteriostatic cleaning agent for pets and a preparation method thereof, and belongs to the technical field of cleaning agents.The bacteriostatic cleaning agent comprises the following components in percentage by weight: 10-20% of a surfactant, 1-5% of borage acid diglyceride monopalmitate, 5-10% of a humectant, 1-5% of a thickening agent, 0.1-0.5% of an odor inhibitor, and the balance of water.The specific compatibility of the borage acid diglyceride monopalmitate and the surfactant has the adsorption effect on dust particles and dirt with positive charges in the hair during cleaning, and the cleaning effect is improved.The repulsion between dirt and the surface of an object is increased, the dispersion of the dirt in water is facilitated, the re-deposition of the dirt is prevented, and the cleaning time is prolonged.

The invention relates to microcapsule powdered oil as well as a preparation method and application thereof. The microcapsule powdered oil comprises the following raw material components in percentage by mass: 82%-92% of oil, 5%-15% of a wall material and 1%-3% of an emulsifier, and the mass content of diglyceride in the oil is not less than 80%; the wall material is formed by compounding sodium caseinate, starch sodium octenylsuccinate, maltodextrin and whey protein, and the mass ratio of the whey protein in the wall material is greater than or equal to 50%; the emulsifier is formed by compounding polyglycerol fatty acid ester, mono-glycerol fatty acid ester, diglycerol fatty acid ester and sucrose fatty acid ester; the preparation method of the microcapsule powdered oil of diglyceride comprises the step of gradient temperature control ultrasonic treatment. According to the invention, the microcapsule powdered oil of diglyceride with high oil content, high embedding rate and small particle size is prepared through cooperation of the specific compounded wall material and the emulsifier and the gradient temperature control ultrasound, and the technical problem that high oil content, high embedding rate and small particle size cannot be realized at the same time is solved.

The invention provides diacylglycerol-rich grease deacidification equipment and a using method thereof.The deacidification equipment comprises an upper barrel and a lower barrel, an oil spraying device is arranged in the upper barrel, an oil outlet is formed in the bottom of the lower barrel, an upper grating, a filler layer, a lower grating and a direct steam device are sequentially arranged in the lower barrel from top to bottom, and the filler layer is arranged in the lower barrel. A feeding door is arranged on the lower barrel body at the upper end of the packing layer, a discharging door is arranged on the lower barrel body at the lower end of the packing layer, and modular assemblies which are vertically stacked are arranged in the packing layer and are loaded into the packing layer through the feeding door. The packing layer is composed of modular assemblies, filling and replacement of the packing layer are facilitated, the upper barrel does not need to be opened wholly, and the replacement efficiency of the packing layer is improved.

The present application provides a frozen beverage and a preparation method thereof. The frozen beverage comprises a milk material containing a milk raw material, a stabilizer and an emulsifier, wherein the stabilizer comprises locust bean gum, xanthan gum and at least one anionic stabilizer; and the emulsifier comprises a monoglyceride and diglyceride and Tween 80. The frozen beverage has significantly improved anti-melting performance and taste.

The invention discloses application of a nutritional composition containing alpha-hydroxyisocaproic acid in weight management and blood fat improvement, and belongs to the fields of biological medicine manufacturing, biological medicine application and functional food development. The nutritional composition comprises alpha-hydroxyisocaproic acid and at least one second functional nutritional component, and the second functional nutritional component is preferably selected from one or more of the following components: 1) one or more of tea polyphenol and capsaicin; 2) one or more of beta-glucan, inulin and resistant dextrin; 3) one or more of medium chain triglyceride and sn-1, 3 diglyceride; under the condition of the same intake cycle and dosage, the nutritional composition can significantly improve the weight management effect and reduce related indexes of blood fat through a multi-way synergistic effect, and is combined with GLP-1 drugs or sequentially applied to weight management so as to reduce the risk of weight rise after drug withdrawal.

The application discloses a detection method for separating diglyceride in vegetable oil and qualitative analysis of the diglyceride, and the diglyceride in the vegetable oil is dissolved by using n-hexane to obtain a sample for standby; different proportion eluent is used to wash and elute a solid phase extraction column, and eluent is collected and dried for standby to obtain eluate; the eluate is analyzed and quantified by using a thin layer chromatography and gas chromatography, and finally, the eluate is finely qualitatively analyzed by using a triple quadrupole; the separation method is simple, the separation of the diglyceride in the vegetable oil can be realized by using two extraction cartridges, the test steps are simplified, the separation purity is high, the cost and time are reduced; finally, qualitative analysis is performed by using GC-MS, the analysis time is short, the specific fatty acid composition in the diglyceride is more finely analyzed, and the qualitative analysis detection of food nutrition and the guidance of related food production are facilitated.

The present application belongs to the field of light industry grease, and discloses a preparation method of diglyceride. The present application carries out pretreatment on the immobilized enzyme carrier, mixes free lipase and glycerol according to a certain proportion, adds the immobilized carrier into the mixed solution of the lipase and glycerol, and carries out immobilization through the adsorption method, so that the immobilized enzyme which has catalytic characteristics and can be used as a reaction substrate is prepared. The obtained immobilized enzyme can be used for the continuous production of diglyceride in a packed bed reactor. Compared with the traditional enzyme method for catalyzing glycerolysis reaction to prepare diglyceride, the immobilized enzyme obtained by the present application not only has catalytic effect, but also has enough glycerol adsorbed on the immobilized enzyme which can be used as a substrate to carry out glycerolysis reaction, so that the reaction efficiency is improved. Not only the problems of difficult recovery and poor reusability of free enzyme are solved, but also the problem of difficult continuous production due to the large viscosity of glycerol in production is solved.

The utility model relates to the technical field of food packaging equipment, in particular to flaxseed diglyceride food packaging equipment which comprises a base and a feeding and discharging mechanism, the feeding and discharging mechanism comprises a mounting plate, a rotating shaft, a rotating plate, two placing plates, a sliding rail and two sliding assemblies, the base is provided with a first sliding groove, and the sliding rail is provided with a second sliding groove. The first sliding groove is communicated with the second sliding groove, the mounting plate is fixedly connected with one end of the base, the rotating shaft is rotationally connected with the mounting plate, the rotating plate is fixedly connected with the rotating shaft and located above the base, the two placing plates are fixedly connected with the two ends of the rotating plate respectively, and the sliding rail is fixedly connected with one end of the base. The two sliding assemblies are arranged at the two ends of the rotating plate correspondingly, packaging boxes are conveyed through the feeding and discharging mechanism, the structure adopted by the feeding and discharging mechanism is simple, the part cost is low, and then the purchase cost of packaging equipment is reduced.

A process for reducing and / or removing diglyceride content without substantial interesterification of triglycerides in an oil comprising steps of providing an oil or fat; and hydrolysis of mono- and diglycerides in said oil with water in the presence of a lipase having at least 80% sequence identity to SEQ ID NO: 1.

The invention discloses hollow mesoporous silica nanoparticle immobilized lipase as well as a preparation method and application thereof in preparation of diglyceride oil. The preparation method comprises the following steps: firstly, preparing a spherical silicon dioxide nanoparticle core as a template, mixing the template, a surfactant, a silicon source and a basic catalyst, carrying out self-assembly and hydrolysis through an intermolecular force, and removing the surfactant to obtain the hollow mesoporous silicon dioxide nanoparticle. Lipase is immobilized on the hollow mesoporous silica nanoparticles in a covalent cross-linking mode, and the hollow mesoporous silica nanoparticle immobilized lipase is obtained. The immobilized lipase has remarkable advantages in the aspects of enzyme catalytic activity retention, enzyme loading rate, mechanical stability and the like, and is good in repeatability and practicability. According to the application of the hollow mesoporous silica nanoparticle immobilized lipase in preparation of diglyceride oil, high-content diglyceride can be obtained, and the hollow mesoporous silica nanoparticle immobilized lipase is high in catalytic efficiency, stable and convenient to recycle.