33 results about "Lipid content" patented technology

A method for inducing an immune response in an individual to treat or prevent a disease or condition is provided, the method comprising administering a vaccine comprising lipid nanoparticles encapsulating nucleic acids encoding antigen proteins, peptides, or fragments thereof, a neutral lipid content of 30% to 70% molar ratio, sterols or derivatives thereof, and 5 to 50% molar ratio of ionizable cationic amino lipids, and a hydrophilic polymer-lipid conjugate present at a lipid content of 0% to 5% molar ratio, wherein administration of the lipid nanoparticles results in an immune response in an individual against the antigen protein, peptide, or fragment thereof expressed by mRNA, wherein each % molar ratio of lipid content relative to the total lipid content of the lipid nanoparticles is measured. This disclosure also provides vaccine compositions and the use of such compositions in inducing an immune response in an individual.

To provide yolk sauce having moderately high viscosity and strong yolk feeling, and capable of imparting complicated taste peculiar to raw egg on rice when mixed with cooked rice.SOLUTION: The yolk sauce according to the present invention contains yolk and albumen, and has a sodium content of less than 0.3 wt%, an egg-derived protein content of 14 to 16 wt%, an egg-derived lipid content of 24 to 28 wt%, and a glycine content of 0.1 to 1.5 wt%, and has a viscosity of 50 to 300Pa·s after freezing and thawing.SELECTED DRAWING: Figure 1

The present invention relates to leguminous protein isolates, particularly pea protein isolates having a low lipid content, as well as to the method for the preparation thereof. In one embodiment, provided is a leguminous plant protein isolate from pea and faba bean wherein said isolate does not contain organic solvent.

The invention discloses a fermentation method for fungus-enzyme synergistic treatment of lucid ganoderma and application of the method in pet food. The fermentation method comprises the following steps: (1) enzymolysis pretreatment: mixing ganoderma lucidum sporocarp powder with water, adding cellulase and laccase, and performing enzymolysis to obtain an enzymolysis solution; (2) sterilizing and supplementing a carbon source: adding the carbon source into the enzymatic hydrolysate, and sterilizing; (3) lactobacillus fermentation: adding lactobacillus plantarum into the sterilized enzymatic hydrolysate, and fermenting under a fermentation condition to obtain fermentation liquor; and (4) solid-liquid separation: performing centrifugal separation on the fermentation liquor, and collecting supernate to obtain the ganoderma lucidum fermentation liquor. According to the method, the laccase and the cellulase are synergistically broken, so that the release efficiency of triterpenes and total phenols in the ganoderma lucidum with spores is remarkably improved. The fermentation liquor can improve lipid accumulation (the oil red O staining lipid content is reduced) and relieve oxidative stress injury on the cell level, and can be used for preparing functional food for improving metabolic health, especially for lipid abnormality and oxidative stress state related to metabolic dysfunction of pets.

To provide a method for producing yolk sauce having moderately high viscosity and strong yolk feeling, and imparting complicated taste peculiar to cooked raw egg on rice when mixed with cooked rice.SOLUTION: The method for producing the egg yolk sauce comprises the following steps: 1. a preparation step of adding raw materials to the filtered liquid egg yolk such that the egg yolk and the egg white have a protein content of 14 to 16% by weight and a lipid content of 24 to 28% by weight, and a sodium content of less than 0.3% by weight and a glycine content of 0.1 to 1.5% by weight, followed by mixing to prepare a preparation liquid, 2. a sterilization step of sterilizing the prepared egg yolk sauce at a temperature of 59.0 °C. or more and 65.0 °C. or less for 3.5 minutes or more, 3. a freezing step of freezing the sterilized egg yolk sauce at - 15 °C. to - 30 °C., and 4. a thawing step of thawing the frozen egg yolk sauce SELECTED DRAWING: Figure 1

Disclosed is a powder and dehydrated product essentially including, or consisting of, a food of plant origin with a lipid content of at least 30% by weight, and a water-soluble bulking agent. Also disclosed is a process for preparing this powder product.

A soft tissue filling composition according to one aspect of the present invention has a high collagen content and a low lipid content, and thus has high biocompatibility and moldability without causing allergic reactions and inflammation. In addition, the composition has a high complex viscosity and storage modulus, and thus can perform an excellent supporting function, maintains homogeneous properties, and can be injected with low injection force by using a standard syringe. Accordingly, the soft tissue filling composition according to one aspect of the present invention can be used to restore the volume of defective or recessed soft tissues, for example, breasts, and can be used for alleviating skin curvature, cosmetic fillers, or supplementing moisture sensation.

This invention relates to a rapid, non-destructive testing method and system for lipid content and deterioration degree in red pine nuts based on hyperspectral imaging and deep learning. The invention belongs to the field of non-destructive testing technology for agricultural and forestry product quality and safety. It addresses the problems of existing methods for detecting lipid content and oxidation degree in red pine nut kernels, which generally suffer from complex operation procedures, high costs for large-scale testing, long processing times, and difficulty in achieving full-process testing during storage and transportation. Key technical points: The original near-infrared spectral data of pine nut samples are obtained through hyperspectral acquisition; the true values ​​of lipid reference content and reference degree of oxidation deterioration of pine nut samples at different sampling times are determined, and a database is established based on these values. The original near-infrared spectral data of red pine nuts are preprocessed, and the data from different batches of red pine nut samples are divided into training and validation sets. An improved one-dimensional dilated convolutional network based on a dynamic weight allocation module is designed to construct a deep learning model suitable for the spectral feature analysis of red pine nut kernels. The constructed model is trained using a backpropagation algorithm, and the network weight parameters are updated in reverse by minimizing the loss function. When the constructed model meets the requirements for rapid detection, it can be used for efficient and non-destructive simultaneous detection of lipid content and oxidative rancidity in red pine nuts.

To provide a leguminous protein isolate, especially a pea protein isolate having a low lipid content, and a method for preparing the same.SOLUTION: Provided is a legume protein isolate characterized in that it contains 7 to 9 grams of total lipids per 100 grams of protein, wherein the legume is a pea.SELECTED DRAWING: None

Provided herein is a lipid nanoparticle comprising an encapsulated oligonucleotide molecule, wherein the oligonucleotide molecule is single-stranded or double-stranded and has a length of between 5 and 500 nucleotides; and 20 to 70 mol % of a neutral lipid content relative to total lipid present in the lipid nanoparticle, an ionizable lipid; a sterol; and optionally a hydrophilic polymer-lipid conjugate.

Provided are methods and compositions for high protein carrierfiller food products derived from fermented biomass and microbially derived proteins.

This invention provides a method for quantitative analysis of intracellular lipid content based on the Bodipy 493 / 503 fluorescent probe, relating to the field of lipid metabolism detection technology. The method includes: (1) determining the absorption spectrum and excitation / emission spectrum of Bodipy 493 / 503 in isopropanol, and determining the maximum absorption wavelength and maximum excitation / emission wavelength of the fluorescent probe in isopropanol based on the spectral results; (2) staining the target cells with the Bodipy 493 / 503 probe after treatment with the compound, observing the changes in fluorescence signal in the field of view before and after isopropanol extraction, and taking pictures; (3) measuring the absorbance and fluorescence intensity of the isopropanol extract, analyzing the experimental results, and comparing them with the results of triglyceride content detection. This quantitative method is simple, fast, sensitive, effective, and has good experimental controllability and repeatability. The analytical results obtained are consistent with those obtained using other lipid content analysis methods, and it can be of great application in the field of research on the lipid metabolism interference effects of chemicals or environmental pollutants.

The application discloses a method for relieving obesity cardiomyopathy lipid toxicity by Chemerin and belongs to the technical field of pharmacological research. Mice are selected to establish an obesity mouse model, and are equally divided into a control group, a high-fat diet group, an AAV CMKLR1 virus group, and a high-fat + AAV CMKLR1 virus group. In succession, mouse heart ultrasound, TSE system detection, glucose tolerance test, and insulin tolerance test are carried out, and mouse blood glucose is measured at intervals. Then, tissue protein is extracted for protein immunoblotting, and tissue RNA is extracted for reverse transcription, and then qPCR is used to detect the expression of related genes. After paraffin embedding and slicing of mouse tissues, HE, WGA, immunofluorescence and other pathological staining are carried out. MTBE method is used for lipid extraction and detection of lipid content. ELISA method is used to measure serum Chemerin, and Acyl-RAC method is used to detect protein palmitoylation. Finally, the experimental results are analyzed to draw a conclusion. The present application proves by clinical data and mouse models that Chemerin compensatorily increases in the process of obesity, and Chemerin can relieve cardiac lipid toxicity caused by obesity and protect cardiac function.

The invention relates to a compound feed for breeding low-fat scophthalmus maximus, and the compound feed comprises 8.1-32.2% of chenopodium quinoa willd germ powder and 36-48% of fish meal on the basis that the dry weight of the compound feed is 100% by weight. On the basis that the dry weight of the compound feed is 100 wt%, the compound feed comprises 8.1-32.2% of quinoa germ powder, 36-48% of fish meal, 12.3-24% of wheat flour, 4.4-9% of vital gluten, 4-4.2% of fish oil, 1-2% of soybean lecithin, 1-2% of vitamins, 1-2% of minerals, 0.4-0.6% of choline chloride, 0.8-1.4% of monocalcium phosphate and 1-3% of squid powder. The chenopodium quinoa is used as a feed protein source to replace fish meal, the dosage of the fish meal can be effectively reduced, the lipid content in fish meat is reduced under the condition of ensuring the growth of fish bodies, and the chenopodium quinoa feed has important significance in solving the problem of aquatic feed protein sources and improving the quality of the fish meat.

The invention relates to the technical field of lipid lowering, in particular to a lipid-lowering composition and a preparation method thereof. Comprising the following raw materials in parts by weight: 0.07-0.09 part of verbena triterpene A, 2-2.5 parts of arteolide and 1-2 parts of bergapten. After verbena triterpene A, arteolide and bergapten are combined, the lipid-lowering effect is accidentally achieved, and it is proved that the fat content of obesity nematodes can be remarkably reduced; a new direction is developed for the utilization of the verbena, and a new thought is provided for the research on weight loss and lipid reduction.

The application discloses a method for reducing the lipid content of oocytes or improving the cryopreservation survival rate of oocytes, and comprises the following steps: (1) preheating an incubation solution; and incubating oocytes in the preheated incubation solution; (2) freezing and thawing the incubated oocytes; wherein the incubation solution is prepared by adding bezafibrate and cholic acid to a culture medium 199 containing 10wt% fetal bovine serum. The incubation solution used in the method provided by the application can significantly reduce the lipid content of oocytes or improve the cryopreservation survival rate of oocytes, compared with the conventional culture medium 199 containing 10wt% fetal bovine serum, because the added bezafibrate and cholic acid have a synergistic lipid-lowering effect. The application has application prospects in reducing the lipid content of oocytes or improving the cryopreservation survival rate of oocytes.

[Problem] The present invention addresses the problem of providing a frozen curry sauce having an excellent formulation balance of proteins, lipids, and carbohydrates. [Solution] The present invention can provide a frozen curry sauce containing glycine and alanine, the frozen curry sauce characterized in that the glycine content is 0.1-6.0 mass%, the alanine content is 0.1-6.0 mass%, and, per 100 g of the frozen curry sauce, the protein content is 8.0-20.0 g, the lipid content is 5.0-15.0 g, and the carbohydrate content is 0-20.0 g.

The invention relates to a method for determining the lipid content in lipid nanoparticles based on RP-UPLC and CAD combination and application. The method comprises the following steps: detecting a lipid nanoparticle test solution and a standard solution by adopting a reversed-phase high performance liquid chromatography and electric fog detector combination method; the detection conditions of the reversed-phase high performance liquid chromatography are as follows: a phenyl column is adopted as a chromatographic column, triethylamine acetate-water is adopted as a mobile phase A, and a triethylamine acetate-acetonitrile solution is adopted as a mobile phase B for gradient elution. The analysis method is good in repeatability and wide in application range, not only can be used for measuring the lipid content in mRNA-LNP, but also is suitable for quantification of lipid in unwrapped nucleic acid drugs, and has important significance for guiding process research and development and formula identification of lipid nanoparticles in the production process.

Compositions comprising Serenoa serrulata (saw palmetto) fruit extract, Laminaria saccharina (brown algae) extract, and Glycyrrhiza inflata root extract are effective inhibit the activity of 5-alpha Reductase; lower neutral lipid content; and reduce expression of SREBP1, FASN and ACACA, in human sebocytes. By regulating excess sebum production, compositions of the invention are useful to improve the health and appearance of skin.

The invention discloses cyclodextrin modified gold nanoparticles and a preparation method and application thereof. The method comprises the steps that a sodium citrate solution and a chloroauric acid solution are sequentially added into water, a sodium borohydride solution is dropwise added under stirring till the solution becomes wine red, and a gold nanoparticle solution is obtained after stirring; and adding the solution of the beta-cyclodextrin derivative with the sulfydryl group into the gold nanoparticle solution, and stirring to obtain the cyclodextrin modified gold nanoparticles. After co-culture of the nanoparticles and cells, the nanoparticles can enter the cells and lipid droplets in the cells, through host-guest interaction and combination of hydrophobic lipids such as cholesterol in the lipid droplets and hydrophobic cavities of cyclodextrin, the lipid is brought out of the cells and enters the next cell to continue to capture the lipid by utilizing the transendocytosis performance of the nanoparticles; the lipid content in cells is obviously reduced; and diseases caused by lipid metabolism disorder are successfully improved.

The invention relates to an application of a chicken CISH gene and an encoding protein thereof in regulating and controlling the crude fat content of yolk. The regulation and control mechanism of the CISH gene on the liver and the relation between the CISH gene and the yolk crude fat content are defined, namely, CISH expression is reduced, a GH / PRL-JAK2-STAT5 negative feedback mechanism is weakened, synthesis of substances such as yolk precursors in the liver is enhanced, and then the substances are transported into the yolk through blood, so that the yolk lipid content is increased. The method for verifying the expression quantity of the CISH gene in the laying hen is adopted, and the lipid deposition level in the egg can be simply and rapidly evaluated. The method not only can provide a new thought for researching yolk lipid deposition, but also lays a foundation for increasing the content of crude fat in the yolk in subsequent production practice.

The present invention relates to a hydrolysate obtained from insects, which contains at least 50% by weight of protein, at least 7% by weight of lipids, and at least 10.5% by weight of ash, these percentages being expressed relative to the dry weight of the hydrolysate, and which has a dry matter content of 15-35% by weight, these percentages being expressed relative to the weight of the hydrolysate. The present invention also relates to a method for preparing the hydrolysate and its use, in particular for improving fish growth.

Provided herein is an oleaginous yeast cell that has been engineered to increase the lipid content with minimal engineering steps. The engineered yeast cells are able to produce yields of lipids that are improved over known yeast cells from a single insertion of an expression cassette. The invention also provides accompanying methods, such as methods of culturing said yeast cells, methods of producing lipids, and accompanying nucleic acids, such as the various nucleic acid constructs, cassettes and genes and proteins for putting the invention into practice.

Compositions comprising Serenoa serrulata (saw palmetto) fruit extract, Laminaria saccharina (brown algae) extract, and Glycyrrhiza inflata root extract are effective inhibit the activity of 5-alpha Reductase; lower neutral lipid content; and reduce expression of SREBP1, FASN and ACACA, in human sebocytes. By regulating excess sebum production, compositions of the invention are useful to improve the health and appearance of skin.

The invention provides a Paeonia ostii or Paeonia fulva derived diacylglycerol acyltransferase 2, and a coding gene and an application thereof, and belongs to the technical field of proteins or functional genes. The encoding gene PoDGAT2 of the Paeonia ostii-derived diacylglycerol acyltransferase 2 and the encoding gene PlDGAT2 of the Paeonia ostii-derived diacylglycerol acyltransferase 2 can effectively biocatalytically synthesize triacylglycerol and increase the total lipid content, and it is also clear that the PoDGAT2 can greatly synthesize C18: 1 delta 9, 9, 9, 10, 11-triacylglycerol under the condition that no fatty acid is used as a substrate or different fatty acids are contained. Alpha-C18: 3 can be better synthesized by PlDGAT2 in an environment in which C18: 1 delta9, C18: 2 and alpha-C18: 3 exist, and C18: 1 delta9 can be preferentially synthesized by PlDGAT2 in other environments. The peony-derived diacylglycerol acyltransferase 2 provided by the invention provides a solid foundation and a theoretical basis for directionally improving the composition of vegetable oil.

This invention discloses an enzymatic method for preparing dairy products rich in functional esters. The method involves mixing and dissolving triglycerides and an active polyhydroxy compound at 45-65°C, then adding an immobilized lipase and the dairy product for transesterification or esterification. Alternatively, the active polyhydroxy compound and whole-fat dairy product are mixed uniformly in a water bath at 45-65°C, an immobilized lipase is added, and after high-speed homogenization, esterification or transesterification occurs. After the reaction, the resulting solution is a semi-product of the functional ester-rich dairy product, which is then subjected to high-pressure homogenization to obtain the final product. This invention significantly improves the nutritional value and functional lipid content of milk and other dairy products without compromising their stability and nutritional components, and also offers advantages such as good storage stability.

Provided is a method for producing a novel soybean-containing processed food with terrine-like (terrine de pate-like) appearance and eating quality made of soybeans as a raw material. The method is configured to comprise: a dough base preparation process P10 of preparing a dough base 1 that forms the basis of a soybean-containing processed food 10 of interest; a secondary material mixing process P20 of mixing a secondary material 2 with the dough base 1 obtained in the dough base preparation process P10; and an aftertreatment process P30 of subjecting a secondary material mixture 3 obtained by the secondary material mixing process P20 to aftertreatment, the aftertreatment process P30 comprising at least a heat treatment step P35 for the secondary material mixture 3, wherein in component composition of the dough base 1, a lipid content is less than a protein content, and a solid content derived from the dough base 1 that accounts for the resulting soybean-containing processed food 10 is 25% by weight or more; and the dough base preparation process P10 comprises a component separation step P15 of separating a coagulated component 04 resulting from coagulation treatment of soymilk 02 from a water-soluble component 06.

The present invention provides improved compositions and processes for preparing mRNA-loaded lipid nanoparticles (mRNA-LNPs); in some embodiments, the present invention provides mRNA-LNPs with exceptional stability and is particularly useful in cases where LNPs comprising low or zero PEG-modified lipid content are desired.

The invention belongs to the technical field of medicines, particularly relates to the crossing field of development of natural active ingredients and prevention and treatment of metabolic diseases, and particularly relates to application of Rk1 in preparation of medicines or functional foods for relieving diseases caused by high fat diet. The ginsenoside Rk1 disclosed by the invention is proved to be orally taken, so that rapid weight increase of mice and excessive white fat expansion caused by high-fat diet can be remarkably reduced, and the lipid content in serum can be reduced; meanwhile, insulin resistance can be effectively relieved, adipose tissue inflammatory response is relieved, and intestinal endotoxemia is prevented by protecting the integrity of the colon barrier. The ginsenoside Rk1 has the potential of serving as a natural therapeutic agent for relieving the diseases caused by the high-fat diet, and a new thought is provided for developing medicines or functional foods for assisting in preventing and treating the diseases caused by the high-fat diet.