60 results about "Lipid content" patented technology

The invention discloses a pretreatment method for determining perfluorinated and polyfluoroalkyl compounds in edible oil. The pretreatment method comprises the following steps: (1) extraction: weighing the edible oil, adding an isotope labeled standard substance, centrifugally extracting PFAS (perfluoroalkyl) in a shaking table, adding desalted water to accelerate lipid sedimentation, and performing refrigerated centrifugation to obtain an extracting solution; (2) enriching and purifying, namely enriching and purifying the extracting solution by using a method of combining liquid-liquid extraction and solid-phase extraction, freezing and centrifuging, and collecting supernate after nitrogen blowing to a constant volume. According to the pretreatment method disclosed by the invention, aiming at the characteristic that the edible oil is rich in protein and lipid content, a liquid-liquid extraction and solid-phase extraction combined method is selected to remove impurities such as protein, lipid and pigment, so that separation and purification of PFAS are realized, and the sample requirements of subsequent LC-MS / MS analysis are met. According to the pretreatment method, the sample amount is increased, the final constant volume is reduced, the detection limit of the method is reduced, the sample treatment speed is increased, rapid analysis can be carried out, and the stability is good.

The invention discloses a traditional Chinese medicine constitution identification method and device, a storage medium and a server. The method comprises the following steps: acquiring a human face spectrum sample and blood fat contents of a tested crowd; inputting the human face spectrum sample into a pre-constructed hyperlipidemia risk prediction model to obtain a target human face spectrum sample with hyperlipidemia in the tested crowd and a first target blood fat four-item content corresponding to the target human face spectrum sample; making corresponding labels on the basis of the target face spectrum samples, corresponding first target blood fat four-item contents and traditional Chinese medicine constitution categories, and training the model through training samples obtained by making the corresponding labels to obtain a traditional Chinese medicine constitution identification model; obtaining a to-be-detected human face spectrum sample, and inputting the sample into the traditional Chinese medicine constitution identification model to obtain the content of four second target blood lipids suffering from hyperlipidemia in the detected crowd and a traditional Chinese medicine constitution identification result corresponding to the content of the four second target blood lipids; the technical problem that enough support cannot be accurately provided for treatment and prevention of patients with the lipemia is solved.

A method for inducing an immune response in an individual to treat or prevent a disease or condition is provided, the method comprising administering a vaccine comprising lipid nanoparticles encapsulating nucleic acids encoding antigen proteins, peptides, or fragments thereof, a neutral lipid content of 30% to 70% molar ratio, sterols or derivatives thereof, and 5 to 50% molar ratio of ionizable cationic amino lipids, and a hydrophilic polymer-lipid conjugate present at a lipid content of 0% to 5% molar ratio, wherein administration of the lipid nanoparticles results in an immune response in an individual against the antigen protein, peptide, or fragment thereof expressed by mRNA, wherein each % molar ratio of lipid content relative to the total lipid content of the lipid nanoparticles is measured. This disclosure also provides vaccine compositions and the use of such compositions in inducing an immune response in an individual.

To provide yolk sauce having moderately high viscosity and strong yolk feeling, and capable of imparting complicated taste peculiar to raw egg on rice when mixed with cooked rice.SOLUTION: The yolk sauce according to the present invention contains yolk and albumen, and has a sodium content of less than 0.3 wt%, an egg-derived protein content of 14 to 16 wt%, an egg-derived lipid content of 24 to 28 wt%, and a glycine content of 0.1 to 1.5 wt%, and has a viscosity of 50 to 300Pa·s after freezing and thawing.SELECTED DRAWING: Figure 1

The invention discloses an application of an oil body protein gene OsOle4 in cultivation of rice strains with different fatty acid contents, the gene specifically regulates and controls the lipid content and cooking taste quality of rice, and does not influence the activity of rice seeds and the growth and development of rice. The OsOle4 is subjected to gene editing and endosperm specific overexpression by adopting a conventional method, so that new rice germplasm with different OsOle4 gene expression levels is obtained. Compared with parent control, the rice germplasm created by the invention has the advantages that the germination rate of seeds, growth and development of plants and basic agronomic traits are not obviously changed, but the rice lipid content of OsOle4 mutant plants is obviously reduced, and the rice lipid content of overexpression plants is obviously increased. Besides, OsOle4 mutation and overexpression plants both show the tendency of cooking taste quality increase, useful gene resources are provided for genetic improvement of rice quality traits, and important breeding utilization value is achieved.

The present invention relates to leguminous protein isolates, particularly pea protein isolates having a low lipid content, as well as to the method for the preparation thereof. In one embodiment, provided is a leguminous plant protein isolate from pea and faba bean wherein said isolate does not contain organic solvent.

The invention discloses a fermentation method for fungus-enzyme synergistic treatment of lucid ganoderma and application of the method in pet food. The fermentation method comprises the following steps: (1) enzymolysis pretreatment: mixing ganoderma lucidum sporocarp powder with water, adding cellulase and laccase, and performing enzymolysis to obtain an enzymolysis solution; (2) sterilizing and supplementing a carbon source: adding the carbon source into the enzymatic hydrolysate, and sterilizing; (3) lactobacillus fermentation: adding lactobacillus plantarum into the sterilized enzymatic hydrolysate, and fermenting under a fermentation condition to obtain fermentation liquor; and (4) solid-liquid separation: performing centrifugal separation on the fermentation liquor, and collecting supernate to obtain the ganoderma lucidum fermentation liquor. According to the method, the laccase and the cellulase are synergistically broken, so that the release efficiency of triterpenes and total phenols in the ganoderma lucidum with spores is remarkably improved. The fermentation liquor can improve lipid accumulation (the oil red O staining lipid content is reduced) and relieve oxidative stress injury on the cell level, and can be used for preparing functional food for improving metabolic health, especially for lipid abnormality and oxidative stress state related to metabolic dysfunction of pets.

The present invention relates to a lipid-lowering peptide and its application. The sequence of the lipid-lowering peptide is: QIF (Gln-Ile-Phe). This lipid-lowering peptide not only has strong bile salt-binding ability, pancreatic lipase inhibitory effect and cholesterol esterase inhibitory effect, but also can effectively reduce the lipid content in cells, reduce the low-density lipoprotein content in cells and increase the high-density lipoprotein content in cells, thus having excellent lipid-lowering activity and can be widely used in lipid-lowering drugs.

To provide a lipid nanoparticle (LNP) for use in the delivery of therapeutic nucleic acids to cells.SOLUTION: Provided is use of (L-Serine) phosphatidylserine (PS) anionic targeting lipid in a total amount of 2.5-10 mol% of the total lipid content of a nucleic acid lipid nanoparticle (LNP) composition in the LNP composition.SELECTED DRAWING: Figure 15

To provide a method for producing yolk sauce having moderately high viscosity and strong yolk feeling, and imparting complicated taste peculiar to cooked raw egg on rice when mixed with cooked rice.SOLUTION: The method for producing the egg yolk sauce comprises the following steps: 1. a preparation step of adding raw materials to the filtered liquid egg yolk such that the egg yolk and the egg white have a protein content of 14 to 16% by weight and a lipid content of 24 to 28% by weight, and a sodium content of less than 0.3% by weight and a glycine content of 0.1 to 1.5% by weight, followed by mixing to prepare a preparation liquid, 2. a sterilization step of sterilizing the prepared egg yolk sauce at a temperature of 59.0 °C. or more and 65.0 °C. or less for 3.5 minutes or more, 3. a freezing step of freezing the sterilized egg yolk sauce at - 15 °C. to - 30 °C., and 4. a thawing step of thawing the frozen egg yolk sauce SELECTED DRAWING: Figure 1

Disclosed is a powder and dehydrated product essentially including, or consisting of, a food of plant origin with a lipid content of at least 30% by weight, and a water-soluble bulking agent. Also disclosed is a process for preparing this powder product.

A soft tissue filling composition according to one aspect of the present invention has a high collagen content and a low lipid content, and thus has high biocompatibility and moldability without causing allergic reactions and inflammation. In addition, the composition has a high complex viscosity and storage modulus, and thus can perform an excellent supporting function, maintains homogeneous properties, and can be injected with low injection force by using a standard syringe. Accordingly, the soft tissue filling composition according to one aspect of the present invention can be used to restore the volume of defective or recessed soft tissues, for example, breasts, and can be used for alleviating skin curvature, cosmetic fillers, or supplementing moisture sensation.

This invention relates to a rapid, non-destructive testing method and system for lipid content and deterioration degree in red pine nuts based on hyperspectral imaging and deep learning. The invention belongs to the field of non-destructive testing technology for agricultural and forestry product quality and safety. It addresses the problems of existing methods for detecting lipid content and oxidation degree in red pine nut kernels, which generally suffer from complex operation procedures, high costs for large-scale testing, long processing times, and difficulty in achieving full-process testing during storage and transportation. Key technical points: The original near-infrared spectral data of pine nut samples are obtained through hyperspectral acquisition; the true values ​​of lipid reference content and reference degree of oxidation deterioration of pine nut samples at different sampling times are determined, and a database is established based on these values. The original near-infrared spectral data of red pine nuts are preprocessed, and the data from different batches of red pine nut samples are divided into training and validation sets. An improved one-dimensional dilated convolutional network based on a dynamic weight allocation module is designed to construct a deep learning model suitable for the spectral feature analysis of red pine nut kernels. The constructed model is trained using a backpropagation algorithm, and the network weight parameters are updated in reverse by minimizing the loss function. When the constructed model meets the requirements for rapid detection, it can be used for efficient and non-destructive simultaneous detection of lipid content and oxidative rancidity in red pine nuts.

The present invention relates to the study of biological mechanisms and belongs to the field of biomedicine. The present invention synthesizes a dual-labeled probe based on coumarin-conjugated bridge-aromatic receptor. The probe molecule can simultaneously and accurately target mitochondria and lipid droplet components with a single molecule. It can be used to fluorescently track the physiological activities of lipid droplets and mitochondria during ferroptosis, achieving the increase in lipid droplet polarity, mitochondrial fibrosis, and ONOO during cell ferroptosis. ‑ This technology allows for the visualization and detection of multiple ferroptosis-related biological information, including lipid content, reduced lipid droplet-mitochondrial contact, and gel-like formation of lipid droplets. This solution addresses the inability of ferroptosis-related drugs to report multiple internal microenvironments of lipid droplets and mitochondria, as well as the connection between organelle microdynamics and ferroptosis, at the organelle level, leading to the inability to visualize and evaluate clinical efficacy. It achieves the potential for evaluating the activity of ferroptosis-inducing drugs and provides an accurate screening tool for the development of drugs that induce ferroptosis mechanisms.

The invention belongs to the field of gene editing, and particularly relates to a method for regulating milk fat content. The expression of genes in fatty acid and cholesterol synthesis pathways of the livers of the animals in the lactation period is specifically regulated and controlled through a gene delivery technology, the expression of liver apolipoprotein B (ApoB) and the triglyceride and total cholesterol level in blood can be remarkably reduced, then the lipid content of milk is effectively reduced, meanwhile, the food intake of the animals is reduced, and the production cost is reduced. And the milk protein content is not influenced. According to the method, milk fat components are accurately controlled through maternal liver gene editing for the first time, a new strategy is provided for breeding healthy dairy animals, and the method has wide application prospects.

To provide a leguminous protein isolate, especially a pea protein isolate having a low lipid content, and a method for preparing the same.SOLUTION: Provided is a legume protein isolate characterized in that it contains 7 to 9 grams of total lipids per 100 grams of protein, wherein the legume is a pea.SELECTED DRAWING: None

Provided herein is a lipid nanoparticle comprising an encapsulated oligonucleotide molecule, wherein the oligonucleotide molecule is single-stranded or double-stranded and has a length of between 5 and 500 nucleotides; and 20 to 70 mol % of a neutral lipid content relative to total lipid present in the lipid nanoparticle, an ionizable lipid; a sterol; and optionally a hydrophilic polymer-lipid conjugate.

Provided are methods and compositions for high protein carrierfiller food products derived from fermented biomass and microbially derived proteins.

This invention provides a method for quantitative analysis of intracellular lipid content based on the Bodipy 493 / 503 fluorescent probe, relating to the field of lipid metabolism detection technology. The method includes: (1) determining the absorption spectrum and excitation / emission spectrum of Bodipy 493 / 503 in isopropanol, and determining the maximum absorption wavelength and maximum excitation / emission wavelength of the fluorescent probe in isopropanol based on the spectral results; (2) staining the target cells with the Bodipy 493 / 503 probe after treatment with the compound, observing the changes in fluorescence signal in the field of view before and after isopropanol extraction, and taking pictures; (3) measuring the absorbance and fluorescence intensity of the isopropanol extract, analyzing the experimental results, and comparing them with the results of triglyceride content detection. This quantitative method is simple, fast, sensitive, effective, and has good experimental controllability and repeatability. The analytical results obtained are consistent with those obtained using other lipid content analysis methods, and it can be of great application in the field of research on the lipid metabolism interference effects of chemicals or environmental pollutants.

The application discloses a method for relieving obesity cardiomyopathy lipid toxicity by Chemerin and belongs to the technical field of pharmacological research. Mice are selected to establish an obesity mouse model, and are equally divided into a control group, a high-fat diet group, an AAV CMKLR1 virus group, and a high-fat + AAV CMKLR1 virus group. In succession, mouse heart ultrasound, TSE system detection, glucose tolerance test, and insulin tolerance test are carried out, and mouse blood glucose is measured at intervals. Then, tissue protein is extracted for protein immunoblotting, and tissue RNA is extracted for reverse transcription, and then qPCR is used to detect the expression of related genes. After paraffin embedding and slicing of mouse tissues, HE, WGA, immunofluorescence and other pathological staining are carried out. MTBE method is used for lipid extraction and detection of lipid content. ELISA method is used to measure serum Chemerin, and Acyl-RAC method is used to detect protein palmitoylation. Finally, the experimental results are analyzed to draw a conclusion. The present application proves by clinical data and mouse models that Chemerin compensatorily increases in the process of obesity, and Chemerin can relieve cardiac lipid toxicity caused by obesity and protect cardiac function.

The invention relates to a compound feed for breeding low-fat scophthalmus maximus, and the compound feed comprises 8.1-32.2% of chenopodium quinoa willd germ powder and 36-48% of fish meal on the basis that the dry weight of the compound feed is 100% by weight. On the basis that the dry weight of the compound feed is 100 wt%, the compound feed comprises 8.1-32.2% of quinoa germ powder, 36-48% of fish meal, 12.3-24% of wheat flour, 4.4-9% of vital gluten, 4-4.2% of fish oil, 1-2% of soybean lecithin, 1-2% of vitamins, 1-2% of minerals, 0.4-0.6% of choline chloride, 0.8-1.4% of monocalcium phosphate and 1-3% of squid powder. The chenopodium quinoa is used as a feed protein source to replace fish meal, the dosage of the fish meal can be effectively reduced, the lipid content in fish meat is reduced under the condition of ensuring the growth of fish bodies, and the chenopodium quinoa feed has important significance in solving the problem of aquatic feed protein sources and improving the quality of the fish meat.

The invention relates to the technical field of lipid lowering, in particular to a lipid-lowering composition and a preparation method thereof. Comprising the following raw materials in parts by weight: 0.07-0.09 part of verbena triterpene A, 2-2.5 parts of arteolide and 1-2 parts of bergapten. After verbena triterpene A, arteolide and bergapten are combined, the lipid-lowering effect is accidentally achieved, and it is proved that the fat content of obesity nematodes can be remarkably reduced; a new direction is developed for the utilization of the verbena, and a new thought is provided for the research on weight loss and lipid reduction.

The invention relates to a method for noninvasively evaluating age-dependent metabolic characteristics of mouse oocytes. The method comprises the following steps: S1, collecting oocytes of mice at different month ages; s2, treating the oocytes to obtain a sample capable of being used for spontaneous Raman spectroscopy and imaging; s3, performing spontaneous Raman spectroscopy and imaging of different oocytes; s4, processing and analyzing Raman spectrum data; s5, processing and analyzing two-dimensional and three-dimensional Raman imaging; s6, analyzing morphological and spectral characteristics of oocytes of mice of different age groups; and S7: determining the specific age-related change of the lipid content and distribution, the cytochrome c level and the metabolic profile in the mouse oocytes. Compared with the prior art, the method has the advantages that the metabolic change of the oocytes of mice with different maternal ages is analyzed by Raman spectrum for the first time, the age-dependent distribution change of lipid and cytochrome c is revealed through high-resolution subcellular mapping, and a new research scheme is provided for the biochemical mechanism of oocyte senescence.

The application discloses a method for reducing the lipid content of oocytes or improving the cryopreservation survival rate of oocytes, and comprises the following steps: (1) preheating an incubation solution; and incubating oocytes in the preheated incubation solution; (2) freezing and thawing the incubated oocytes; wherein the incubation solution is prepared by adding bezafibrate and cholic acid to a culture medium 199 containing 10wt% fetal bovine serum. The incubation solution used in the method provided by the application can significantly reduce the lipid content of oocytes or improve the cryopreservation survival rate of oocytes, compared with the conventional culture medium 199 containing 10wt% fetal bovine serum, because the added bezafibrate and cholic acid have a synergistic lipid-lowering effect. The application has application prospects in reducing the lipid content of oocytes or improving the cryopreservation survival rate of oocytes.

This vacuum freeze-dried block for sauce contains a solid or liquid vegetable oil / fat having a melting point of 0-50°C, an emulsifier, and a paste selected from processed starch and gelatin. The lipid content of the block is 40-90 mass%. The content of the paste is preferably 1.0-15.0 parts by mass based on 100 parts by mass of the lipid. It is also preferable to contain the processed starch. The emulsifier is preferably one or more selected from sorbitan fatty acid ester and glycerin fatty acid ester.

The invention provides a method for improving production and freezing efficiency of in-vitro embryos of cattle and sheep, which comprises the following steps of: adding fuchsin, linoleic acid and Mg < 2 + > into an IVC culture medium, and detecting the developmental capacity, freezing survival rate, lipid content, embryonic cell apoptosis level, ROS level, cytoskeleton and the like of the embryos; the invention is helpful for establishing an effective method to reduce the lipid content of the cattle and sheep embryos, thereby improving the developmental ability of the vitrification frozen cattle and sheep embryos.

The invention provides lipid bodies isolated from yeast, compositions comprising the lipid bodies, products made from the lipid bodies, methods of making the lipid bodies, and methods of using the lipid bodies. The lipid bodies of the invention have an exceptionally large size and high internal neutral lipid content, providing a number of advantages for a variety of practical applications.

[Problem] The present invention addresses the problem of providing a frozen curry sauce having an excellent formulation balance of proteins, lipids, and carbohydrates. [Solution] The present invention can provide a frozen curry sauce containing glycine and alanine, the frozen curry sauce characterized in that the glycine content is 0.1-6.0 mass%, the alanine content is 0.1-6.0 mass%, and, per 100 g of the frozen curry sauce, the protein content is 8.0-20.0 g, the lipid content is 5.0-15.0 g, and the carbohydrate content is 0-20.0 g.

The invention relates to a method for determining the lipid content in lipid nanoparticles based on RP-UPLC and CAD combination and application. The method comprises the following steps: detecting a lipid nanoparticle test solution and a standard solution by adopting a reversed-phase high performance liquid chromatography and electric fog detector combination method; the detection conditions of the reversed-phase high performance liquid chromatography are as follows: a phenyl column is adopted as a chromatographic column, triethylamine acetate-water is adopted as a mobile phase A, and a triethylamine acetate-acetonitrile solution is adopted as a mobile phase B for gradient elution. The analysis method is good in repeatability and wide in application range, not only can be used for measuring the lipid content in mRNA-LNP, but also is suitable for quantification of lipid in unwrapped nucleic acid drugs, and has important significance for guiding process research and development and formula identification of lipid nanoparticles in the production process.

The present invention relates to the field of oat protein compositions and methods for their production. Specifically, the present invention relates to oat protein compositions having a low lipid content and free of trace amounts of organic solvents and methods for their production.