Multidimensional mass spectrometry of serum and cellular lipids directly from biologic extracts

Abstract

A method for determination of at least one of the lipid species in a biological sample comprising subjecting the sample to lipid extraction to obtain a lipid extract and subjecting the resulting lipid extract to multidimensional electrospray ionization mass spectrometry using either precursor ion or neutral loss scanning (or both) of all naturally occurring aliphatic chains, lipid fragments and precursor ions leading to observed fragments to generate a multidimensional matrix whose contour densities provides structural and quantitative information directly without chromatography. A method for determination of lipid content and / or lipid molecular species composition and quantity directly from lipid extracts of a biological sample comprising subjecting said lipid extract to electrospray ionization multidimensional mass spectrometry by comparisons to standards and algorithms described herein.

Description

CROSS REFERENCE TO RELATED APPLICATION[0001]This application is a continuation in part of pending U.S. nonprovisional patent application Ser. No. 10 / 606,601, filed Jun. 26, 2003 now U.S. Pat. No. 7,306,952 which claims priority to U.S. provisional patent application 60 / 391,711 filed Jun. 26, 2002, and claims the benefit of U.S. provisional patent application 60 / 458,733 filed Mar. 28, 2003 all of which are incorporated by reference in their entirety.STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH & DEVELOPMENT[0002]This work was supported by grants from NIH including grants W / H P01 HL57278 / JDFI 996003, R02HL41250, RO1 AA11094, P41-RR00954, P60-DK20579, and P30-DK56341. The government has certain rights in the invention.FIELD OF THE INVENTION[0003]This invention relates generally to a method of analysis for lipids including triglycerides and other mentioned cellular lipids in a biological sample. More particularly this invention relates to a method for analysis and individual molecul...

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Benefits of technology

[0015]Lipids are essential cellular constituents that have multiple distinct yet critical roles in cellular function. Lipids provide an impermeable barrier which separate intracellular and extracellular compartments without which life and self-renewal would be impossible. Moreover, lipids concurrently provide a matrix for the appropriate interactions of membrane-associated proteins to interact with each other as well as promote interactions of membrane proteins with cognate intra- and extracellular binding partners. Finally biologic membranes serve as storage reservoirs for biologically active 2nd messengers (eicosanoids, diglycerides, ceramides, etc.) that allow each cell to effectively respond to internal and external stimuli. Biologic membranes fulfill these multiple functions through the synthesis of multiple distinct covalent entities each with its unique structural and physical characteristics. The inherent chemical diversity present in biologic lipids is achieved through multiple discrete covalent assemblies of lipid backbone (typically glycerol) with linear combinations of various aliphatic chains (typically 14-22 carbons long containing variable amounts of unsaturation). This biologic diversity facilitates the specific tailoring of individual cellular responses to alterations in cellular nutrient status, metabolic history and signaling events. Accordingly, many groups have rigorously pursued the identification of alterations in cellular lipid constituents to identify the chemical mechanisms underlying such diverse diseases as obesity, atherosclerosis and lipotoxicity now endemic in industrialized populations.

Problems solved by technology

Additionally, such afflictions destroy or significantly reduce the quality of life even if not immediately fatal.

Although some studies have measured total TG molecular species content in multiple different disease states, a paucity of information on TG molecular species changes during pathophysiological alterations is available.

Previous attempts at direct TG quantitation by positive-ion electrospray ionization mass spectrometry (ESI / MS) were undesirably confounded by the presence of overlapping peaks from choline glycerophospholipids requiring chromatographic separation of lipid extracts prior to ESI / MS analyses.

Moreover, isobaric molecular species present in all biological tissues prevent determination of individual molecular species of triglycerides from molecular weight determinations alone.

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