25 results about "Cellular lipid" patented technology

This invention belongs to the field of biomedical technology, specifically relating to a compound extracted from *Acer buergerianum* (a type of shrub), its extraction method, and its application in the preparation of drugs for the prevention or treatment of non-alcoholic fatty liver disease. This invention is the first to obtain a natural compound with a novel structure from *Acer buergerianum*, and provides a method for its preparation. Activity studies were conducted, and experimental data show that the compound exhibits low cytotoxicity and no significant cytotoxicity to normal cells. It can inhibit the expression of fatty acid synthases ACC, ACLY, and FAS, thereby inhibiting de novo fatty acid synthesis in cells, suppressing lipid droplet accumulation, and reducing cellular lipid levels. This compound has broad application prospects in the preparation of drugs for the prevention or treatment of non-alcoholic fatty liver disease.

The invention relates to a full-automatic high-flux high-coverage single-cell lipidomics analysis device and method, and the device comprises a valve which is provided with a quantitative groove; the cell suspension injection assembly is communicated with the sample inlet of the quantitative tank through a capillary tube; the capillary chromatographic column spray needle comprises a body and a tip end, the body is communicated with the chromatographic column connector of the quantitative groove, the tip end is aligned with a sample injection conical opening of the mass spectrometer, the capillary chromatographic column spray needle is filled with filler, the capillary chromatographic column spray needle is sleeved with a metal tube, and the metal tube is connected with the capillary chromatographic column spray needle. An electric field is applied to the metal tube in time; the supercritical fluid injection part is communicated with the mobile phase inlet of the quantitative tank and is used for injecting supercritical fluid into the quantitative tank, so that a single cell sample is extracted on line. In a single experiment, the device can quickly obtain the number of analysis samples with statistical significance.

The present invention relates to the field of natural medicine and pharmaceutical technology, and discloses a new compound, novofuran, prepared from Nocardia spp., as well as its preparation method and application. The present invention isolated Nocardia spp. ZHD001 for the first time from sediments collected from the sea area surrounding Zhoushan, and further isolated and studied the fermentation product of Nocardia spp. ZHD001 to obtain novofuran. The present invention determined the chemical structure of the new compound novofuran by analyzing high-resolution mass spectrometry (HRESIMS), hydrogen spectrum, carbon spectrum, HMQC spectrum, HMBC spectrum, and COSY spectrum data of novofuran. A series of studies have shown that novofuran can significantly reduce lipid accumulation in a HepG2 cell lipid model, exhibits lipid-lowering biological activity, and can be used to prepare lipid-lowering drugs or lipid-lowering health foods, showing excellent development and application prospects.

The invention discloses a transmembrane protein 16F and application of a gene coded by the transmembrane protein 16F as a target spot in preparation of a medicine for regulating and controlling tumor ferroptosis. The tumor is any one of lymphoma, melanoma, colon cancer, cervical cancer, fibrosarcoma and ovarian epithelial cancer. The invention finds that TMEM16F is a ferroptosis inhibiting factor which does not change a cell lipid peroxidation process, and shows that the spatial distribution of oxPLs can regulate ferroptosis. In the ferroptosis process, the TMEM16F-mediated phospholipid overturning process is activated, and the effect of regulating and controlling phospholipid redistribution is achieved. In addition, the combination of TMEM16F defect and anti-PD-1 treatment shows a synergistic anti-tumor effect, and initiates a strong tumor immunological rejection reaction. The ivermectin has the biological activity of inhibiting the overturning function of TMEM16F phospholipid, and the inhibition effect of the ivermectin on TMEM16F effectively enhances the reactivity of anti-PD-1 treatment.

The present invention discloses a tuna swim bladder hypolipidemic and antioxidant oligopeptide and its application. The present invention uses tuna swim bladder as a raw material, and after enzymatic hydrolysis, ultrafiltration, gel chromatography purification, and RP-HPLC purification, the tuna swim bladder hypolipidemic and antioxidant oligopeptide His-Ser-Gly-Pro-Tyr-Met (HSGPYM) is prepared, and its molecular weight is 690.8 Da. The HSGPYM of the present invention can significantly reduce the contents of triglyceride (TG) and total cholesterol (TC) in the lipid accumulation model of HepG2 cells, showing good lipid-lowering activity; at the same time, it can significantly increase the antioxidant enzyme level in the cell model, effectively scavenge excessive reactive oxygen species, and reduce oxidative stress damage. The HSGPYM of the present invention has the advantages of being safe, non-toxic, and having strong lipid-lowering and antioxidant capabilities, and can be used for the development of functional products for the treatment of non-alcoholic fatty liver disease, and can also be used as an additive for health products and foods.

The invention discloses an application of N-trans-feruloyltyramine in treatment of a non-alcoholic fatty liver disease. The invention further discloses a verification method for proving that the N-trans-feruloyltyramine is used for treating the non-alcoholic fatty liver disease. The invention further discloses a medicine for treating the non-alcoholic fatty liver disease. The invention discloses N-trans-feruloyl tyramine which can be applied to prevention or treatment of NAFLD (non-alcoholic fatty liver disease). In-vitro cell experiments find that N-trans-feruloyltyramine can reduce lipid deposition and oxidative stress damage of HepG2 cells induced by OA / PA, reduce the level of TC and TG in the cells by promoting efflux of TC and TG in the cells, reduce expression of MDA in the cells and promote expression of SOD, CAT and GSH in the cells; in-vivo animal experiments show that the N-trans-feruloyltyramine can reduce the levels of TC, TG and LDL-C in serum, reduce the levels of TC, TG, LPO, ALT and AST in liver, and relieve infiltration of inflammatory cells in liver tissue.

The present disclosure provides lipid nanoparticles that are particularly beneficial in inducing an immune response in a subject, particularly by stimulating CD8+ T cells associated with protein or protein fragments of interest. The lipid nanoparticles can have a negative zeta potential and / or particular configurations and / or relative amounts of a phospholipid, an ionizable lipid, and a conjugated lipid. The disclosure also provides vaccines, other pharmaceutical compositions, and methods including the provided lipid nanoparticles.

This invention relates to a compound extracted from *Dryopteris macrocarpa*, its extraction method, and its application in the preparation of drugs for the prevention and / or treatment of non-alcoholic fatty liver disease. This invention provides a novel natural compound obtained from *Dryopteris macrocarpa* for the first time, along with a method for its preparation. Activity studies were conducted, and experimental data show that the compound exhibits low cytotoxicity to normal cells. It can inhibit fatty acid uptake in cells by suppressing the expression of fatty acid uptake-related genes FATP2 and CD36, thereby inhibiting lipid droplet accumulation and reducing cellular lipid levels. This compound can be applied in the preparation of drugs for the prevention and / or treatment of non-alcoholic fatty liver disease.

This invention discloses refined mulberry seed oil, its extraction method, and its application. Compared with the prior art, the advantages are: (1) The method of this invention uses agricultural waste (mulberry seeds) as raw material, and obtains mulberry seed oil through low-moisture enzymatic hydrolysis pretreatment combined with high-temperature pressing. The enzyme dosage is low (0.1%~1.0%), ethanol is recycled, solvent loss is small, the process is simple, the operation is convenient, and it is easy to realize industrial-scale production; (2) The mulberry seed oil prepared by this invention has an unsaturated fatty acid content of up to 88.26%, of which the linoleic acid content is 81.6%; (3) The mulberry seed oil of this invention shows significant lipid-regulating activity in both cell and nematode models: it can improve the lipid content index and protein expression of hyperlipidemic cells, and it can also regulate nematode-related genes and reduce lipid accumulation; (4) This invention uses mulberry seeds, a waste of silkworms, as raw material, and realizes the high-value utilization of agricultural by-products, which has good industrial application prospects and economic and social benefits.

The present invention provides a polypeptide for inhibiting fat accumulation and its use. Its amino acid sequence is MNGLTLGSIPKEEAEKERQGWLEAAK. This polypeptide can inhibit CD36 transport of extracellular fatty acids, reducing cellular lipid uptake and accumulation, and effectively inhibiting the accumulation of intracellular triglycerides and other fats caused by free fatty acids.

The present application relates to the technical field of compounds, in particular to a natural active compound pseudo-norfurane, a preparation method and application thereof. The present application provides a natural active compound pseudo-norfurane, which has the structure shown in the following formula (I), the compound formula (I) of the present application can significantly reduce the lipid accumulation in a HepG2 cell lipid model, has a hypolipidemic biological activity, can be used for preparing a hypolipidemic drug or a hypolipidemic health food, and has a good development and application prospect.

The invention discloses a construction method of a hepatocyte lipid accumulation model and application of the hepatocyte lipid accumulation model in screening of lipid-lowering functional substances, and belongs to the technical field of detection. The method comprises the following steps: culturing AML12 cells by using a DMEM / F-12 culture medium containing 10% of FBS, 1 * ITS and 1% of P / S, carrying out combined intervention for 24-72 hours by using 2%-3% of ethanol and 50-100 [mu] M of palmitic acid for molding, and quantifying the intracellular lipid accumulation degree through BODIPY fluorescence detection. The model simulates pathological mechanisms of alcoholism and high fat diet, AML12 cells retain key enzymes of alcohol metabolism, the cell viability is maintained at 60%-80% after modeling, and the lipid fluorescence intensity is remarkably improved compared with that of a blank group. The model can efficiently screen lipid-lowering and liver-protecting functional substances for relieving alcoholic fatty liver, is sensitive and quantifiable in detection, and provides a reliable tool for screening related medicines and extracts.

Polynucleotides such as DNA are stored inside vesicles formed from self-assembling membranes. The vesicles may be protocells, liposomes, micelles, colloidosomes, proteinosomes, or coacervates. The vesicles may include surface functionalization to improve polynucleotide encapsulation and / or to bind polynucleotides having specific sequences. Encapsulation in vesicles provides protection for the polynucleotides. Additional protection is provided by addition of one or more stabilizers. The stabilizer may be nucleic-acid stabilizers that stabilize the polynucleotides or may be a protective structural layer around the vesicles such as a layer of silica. A process for stably storing polynucleotides in vesicles and a process for recovering stored polynucleotides from vesicles are both disclosed. The polynucleotides may be used for storage of digital information.

The application discloses the use of ACY-1 modulators as a therapeutic target for fatty liver disease and drug screening. By constructing a HepG2 cell lipid deposition model, it is found for the first time that ACY1 overexpression can exacerbate cell triglyceride accumulation; by constructing a MASH mouse model induced by a high-fat high-cholesterol high-fructose diet, it is found that ACY1 overexpression can exacerbate MASH progression; based on these findings, a drug screening method for preventing or treating fatty liver disease is constructed, and 2-hydroxyhexanoic acid (2-HHA) is screened out, which can significantly inhibit the in vitro enzyme activity of ACY1, and can alleviate abnormal cell triglyceride accumulation caused by palmitic acid-oleic acid stimulation at the cell and animal levels, and significantly improve the weight gain, liver lipid deposition, fatty degeneration, inflammatory infiltration and fibrosis of MASH mice induced by a high-fat high-cholesterol high-fructose diet, and the like, in addition, the inhibitor 2-hydroxyhexanoic acid can significantly reduce the abnormally increased serum ALT, AST and liver TC and TG of MASH mice.

A super-bright fluorescent probe for cellular lipid droplets and its application in the preparation of bioimaging reagents, belonging to the field of bioimaging technology. Aiming at the deficiencies of the existing fluorescent imaging probes for cellular lipid droplets in terms of insufficient photostability and insufficient selectivity for lipid droplet staining, the present invention selects a fluorescent molecule based on stilbene derivatives in the development of a new type of fluorescent probe for lipid droplet imaging. While exhibiting high photostability, this fluorescent molecule has excellent selectivity for lipid droplet staining. This fluorescent probe can be used to prepare reagents for specifically labeling lipid droplets in cells, visually tracking the dynamic trajectories of cellular lipid droplets, and quantitatively measuring the movement speed of cellular lipid droplets. The fluorescent probe Lipi-Bright described in the present invention has excellent photostability and staining selectivity. Under the same staining and imaging conditions, Lipi-Bright exhibits more excellent photostability and staining selectivity than commercial fluorescent probes for lipid droplets, and has great application prospects.

The invention discloses a type I 5 alpha-reductase inhibitory peptide as well as a composition and application thereof, and the type I 5 alpha-reductase inhibitory peptide can be used for remarkably inhibiting expression increase of a type I 5 alpha-reductase gene, abnormal expression of a lipid synthesis related gene and abnormal expression of an inflammatory factor gene caused by androgen and remarkably inhibiting lipid generation of cells. The compound can be used for preparing medicines, cosmetics, skin care products, food or health care products, can be used as an oil-control component or an anti-acne component, and has a wide application prospect.

The application discloses a traditional Chinese medicine polysaccharide with lipid-lowering activity and a preparation method and application thereof, relates to the technical field of biological medicine, and the traditional Chinese medicine polysaccharide is neutral uniform polysaccharide ACP1 in allium chinense, the weight-average molecular weight is 17.96 kDa, the polydispersity index is 1.726, is composed of glucose and fructose, and the molar ratio is 2.53%:97.47%. The neutral uniform polysaccharide in allium chinense provided in the application is a new neutral uniform polysaccharide ACP1 extracted from allium chinense, can effectively improve the lipid deposition of AML-12 cells induced by oleic acid, has significant lipid-lowering activity, and is high in safety. The preparation method of the neutral uniform polysaccharide in allium chinense provided in the application is good in repeatability, the obtained neutral uniform polysaccharide is high in purity, and is beneficial to industrial production.

This disclosure provides methods of increasing in vivo transfection efficiency and pharmacologic activity of T cells, by administering multiple small doses within a compact time period of T cell-targeted lipid nanoparticles encapsulating mRNA encoding an antigen receptor that recognizes an antigen of a cell against which immune activity is to be directed. Also provided are methods of depleting B cells, and methods of treating B cell-mediated diseases and disorders by depleting B cells and achieving immunological reset, entailing administration of immune cell-targeted lipid nanoparticles encapsulating mRNA encoding an antigen receptor recognizing a B cell marker as multiple small doses within a compact time period. The antigen receptor can be a T cell receptor or a chimeric antigen receptor.

Polynucleotides such as DNA are stored inside vesicles formed from self-assembling membranes. The vesicles may be protocells, liposomes, micelles, colloidosomes, proteinosomes, or coacervates. The vesicles may include surface functionalization to improve polynucleotide encapsulation and / or to bind polynucleotides having specific sequences. Encapsulation in vesicles provides protection for the polynucleotides. Additional protection is provided by addition of one or more stabilizers. The stabilizer may be nucleic-acid stabilizers that stabilize the polynucleotides or may be a protective structural layer around the vesicles such as a layer of silica. A process for stably storing polynucleotides in vesicles and a process for recovering stored polynucleotides from vesicles are both disclosed. The polynucleotides may be used for storage of digital information.

The invention discloses a traditional Chinese medicine polysaccharide with lipid-lowering activity and a preparation method and application thereof, and relates to the technical field of biological medicine, the traditional Chinese medicine polysaccharide is allium macrostemon neutral homogeneous polysaccharide ACP1, the weight-average molecular weight is 17.96 kDa, the polydispersity index is 1.726, and the traditional Chinese medicine polysaccharide is composed of glucose and fructose according to the molar ratio of 2.53%: 97.47%. The allium macrostemon neutral homogeneous polysaccharide provided by the invention is a new neutral homogeneous polysaccharide ACP1 extracted from allium macrostemon, can effectively improve oleic acid-induced AML-12 cell lipid deposition, and has remarkable lipid-lowering activity and high safety. The preparation method of the allium macrostemon neutral homogeneous polysaccharide is good in repeatability, the obtained neutral homogeneous polysaccharide is high in purity, and industrial production is facilitated.

The invention provides a method and a device for screening membrane proteins. The device comprises a first fusion protein comprising a membrane protein present in a boundary layer (e.g., the wall of a cell, liposome, or vesicle) and fused to one member of a binding pair, and a second fusion protein comprising an intracellular ligand fused to the membrane protein of the other member of the binding pair, wherein the combination pair is capable of generating a detectable signal.

The invention relates to the technical field of biological analysis, and discloses a single-cell lipid type characteristic driven cell typing method, system, equipment and medium. The cell lipid type is defined by using single-cell lipid data, a cell lipid type library can be established, labeling is not needed, the method is suitable for any biological cell sample, and the applicability and subsequent cell typing efficiency of the method are greatly improved; the cell lipid type library can be repeatedly used after being established, and pure cells do not need to be obtained in each experiment, so that the cost can be effectively reduced; secondly, the cell sample is accurately divided into different subgroups according to cell lipid characteristics by using an unsupervised clustering method, finally, different subgroups in the cell sample are identified by using lipid type definitions of different cells, and meanwhile, unmatched novel lipid subgroups are found, so that the cell type of a molecular level can be stably described; and the cell typing precision is improved. In addition, an experience-driven research mode is changed into a data-driven research mode, so that researchers can be helped to quickly find out neglected biological discovery.

This invention discloses a method for extracting lipid components from dermal adipocytes and its application, comprising the following steps: (1) extracting primary dermal fibroblasts from mice on day 0 of birth; (2) culturing the primary dermal fibroblasts in DMEM+++ medium until the cell adhesion density reaches 100%, then inducing adipogenic differentiation in vitro using adipogenic differentiation medium, and then maintaining the culture in maintenance medium until the cells are in an early adipogenic state, and separating the supernatant of the early differentiated cells; (3) extracting and separating the supernatant obtained in step (2) using the Matyash method to obtain the lipid components of the dermal adipocytes. This invention, by extracting and separating lipid and protein components from the supernatant of early differentiated adipocyte culture, obtains lipid components with significant immunomodulatory functions, which can significantly inhibit the inflammatory state of macrophages and the proliferation of keratinocytes.

The invention relates to a gene target spot and a medicine for treating glycolipid metabolism related diseases, and belongs to the technical field of biological medicines. The invention provides application of an ARPP21 gene and / or protein as a target spot in preparation of drugs for preventing and / or treating diseases related to glycolipid metabolism. The invention finds that the weight of an ARPP21 knockout mouse becomes lighter, visceral organs are injured, and compared with a wild mouse, the liver has fat deposition and lipid metabolism related factor expression is up-regulated. According to the present invention, by constructing the AML-12 cell capable of overexpressing and knocking down the Arpp21, the expression of the lipid metabolism related gene of the AML-12 cell capable of knocking down the ARPP21 is increased, and after the cell lipid deposition is induced, the adiposis of the AML-12 cell capable of overexpressing the Arpp21 is reduced; according to the invention, the function and mechanism of the ARPP21 participating in glycolipid metabolism pathways in the liver and muscles are defined, and a new therapeutic target and strategy are provided for glycolipid metabolic diseases.

The invention belongs to the technical field of biological medicines, particularly relates to application of PLIN3 in preparation of a medicine for improving or inhibiting foaming of vascular smooth muscle cells, and provides a new strategy for improving foaming of the vascular smooth muscle cells. Vascular smooth muscle cell foaming is a key process of atherosclerosis development. The exploration process of the PLIN3 for improving vascular smooth muscle cell foaming comprises the following steps: 1) constructing a foamed cell model; 2) screening and determining treatment targets; and 3) the specific effect of the PLIN3 in regulating and controlling the lipid homeostasis of the vascular smooth muscle cells. Researches find that the knock-down of the PLIN3 can effectively reduce lipid accumulation of vascular smooth muscle cells, the over-expression of the PLIN3 obviously promotes intracellular lipid accumulation, and the PLIN3 plays an important role in maintaining a cell lipid steady state. Therefore, the medicine targeting the PLIN3 has application potential in the aspect of preventing and treating the atherosclerosis and other lipid metabolism syndromes.