Method for purifying main-effect phenolic ingredients inhibiting hepatic cell lipid accumulation in litchi pulp

A technology of lychee pulp and purification method, which is applied in the field of food processing, can solve problems such as the synergistic effect of easily overlooked compounds, the cumbersome and time-consuming polyphenol active ingredients of lychee pulp, etc., and achieve the effect of regulating lipid metabolism and inhibiting hepatic steatosis

Active Publication Date: 2016-11-16
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defects of the prior art, the present invention is to overcome the deficiencies of the prior art, provide a simplified purification method of the main effect phenolic components in the litchi pulp that inhibits liver cell lipid aggregation, and solve the problem of separating the compounds one by one to prepare litchi pulp. Phenolic active ingredients are cumbersome and time-consuming, and it is easy to overlook the problem of synergistic effects between compounds

Method used

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  • Method for purifying main-effect phenolic ingredients inhibiting hepatic cell lipid accumulation in litchi pulp
  • Method for purifying main-effect phenolic ingredients inhibiting hepatic cell lipid accumulation in litchi pulp
  • Method for purifying main-effect phenolic ingredients inhibiting hepatic cell lipid accumulation in litchi pulp

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Embodiment 1

[0050] A method for purifying main-effect phenolic components in litchi pulp for inhibiting liver cell lipid aggregation, comprising the steps of:

[0051] S1, preparing litchi pulp polyphenol extract, comprises the following steps:

[0052] S11. Preparation of litchi pulp polyphenol crude extract: soak fresh litchi pulp in 95% edible alcohol pre-cooled at 4°C, homogenize, collect the supernatant after centrifugation, recover ethanol under reduced pressure at 45°C, and recover the remaining aqueous solution Litchi pulp polyphenol crude extract;

[0053] S12. After the litchi pulp polyphenol crude extract obtained in step S11 is adsorbed by AB-8 macroporous resin, it is eluted with distilled water to remove soluble sugar and other small molecular compounds, and then eluted with 70% ethanol, and the ethanol is collected The eluate is concentrated under reduced pressure at 45°C to obtain a dark yellow concentrated solution, which is the polyphenol extract of litchi pulp after va...

Embodiment 2

[0075] A method for purifying main-effect phenolic components in litchi pulp for inhibiting liver cell lipid aggregation, comprising the steps of:

[0076] S1, preparing litchi pulp polyphenol extract, comprises the following steps:

[0077] S11. Preparation of litchi pulp polyphenol crude extract: soak fresh litchi pulp in 95% edible alcohol precooled at 0°C, the ratio of fresh litchi pulp to 95% edible alcohol is 1:3w / v, homogenize, centrifuge Finally, the supernatant was collected, and the ethanol was recovered under reduced pressure at 55°C, and the remaining aqueous solution was the crude extract of litchi pulp polyphenols;

[0078] S12. After the litchi pulp polyphenol crude extract obtained in step S11 is adsorbed by AB-8 macroporous resin, it is eluted with distilled water to remove soluble sugar and other small molecular compounds, and then eluted with 65% ethanol, and the ethanol is collected for washing. Deliquified, concentrated under reduced pressure at 55°C to o...

Embodiment 3

[0085] Except that the volume percentage of ethanol used for elution in step S12 is 75%, the others are the same as in Example 1.

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Abstract

The invention discloses a method for purifying main-effect phenolic ingredients inhibiting hepatic cell lipid accumulation in litchi pulp. Four different phenolic ingredients like procyanidine and flavonoid substance in the litchi pulp are separated to realize inhibiting effect of the four phenolic ingredients on oleic-acid-induced lipid accumulation in hepatic cells.

Description

technical field [0001] The invention relates to the field of food processing, in particular to a method for purifying main-effect phenolic components in litchi pulp for inhibiting liver cell lipid aggregation. Background technique [0002] Litchi is the main characteristic fruit in subtropical regions. Because of its bright and beautiful peel, delicate and sweet gravy, it is deeply loved by consumers at home and abroad, and is known as "the treasure of fruit". my country is the main producer of lychees, and its planting area and output both rank first in the world. As the main producing area of ​​lychee, Guangdong Province accounts for more than 60% of the total lychee output in the country. Due to the concentrated time of lychees on the market and the short preservation time, the fresh sales market is under great pressure. How to carry out intensive processing of lychees, alleviate the pressure on the fresh market, and increase the added value of lychees has become an urg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/77A61P3/06A61P1/16A23L19/00A23L33/00
CPCA23V2002/00A61K36/77A61K2236/333A61K2236/55A23V2200/32A23V2200/326A23V2250/2132
Inventor 张瑞芬董丽红张名位肖娟邓媛元黄菲魏振承张雁马永轩唐小俊
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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