Methods for Elevating Fat/Oil Content in Plants

a technology of fat/oil content and plant cells, applied in the direction of transferases, peptide sources, enzymology, etc., can solve the problems of low abundance of lipid droplets, difficult production of oils in non-seed plant tissues for industrial applications, and insufficient understanding of transient accumulation of stored oils in non-seed tissues, etc., to improve the lipid content of genetically modified plant cells, increase the lipid content of cellular lipid droplets, and reduce lipa activity

Inactive Publication Date: 2015-11-19
BOSTON MEDICAL CENTER INC +1
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Vegetable oils can be synthesized in non-seed (such as leaf) tissues; however, their abundance is low and the stored lipids are presumed to be metabolized rapidly, perhaps for the recycling of fatty acids for energy or the synthesis of membrane lipids.
Currently, the regulation and transient accumulation of stored oils in non-seed tissues are not well understood, and the production of oils in non-seed plant tissues for industrial applications remains challenging.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods for Elevating Fat/Oil Content in Plants
  • Methods for Elevating Fat/Oil Content in Plants
  • Methods for Elevating Fat/Oil Content in Plants

Examples

Experimental program
Comparison scheme
Effect test

example 1

Increase of Lipid Content and Induction of Lipid Droplet Formation in Plants Using Mammalian Proteins Associated with Lipid Metabolism

[0328]Plant transformation vectors are constructed and are propagated in Eschericia coli Top 10 cells. The vectors are sequenced for verification. Plasmid vectors are transformed into Agrobacterium, tunefaciens LBA4404, and the clones are selected and verified by PCR. Arabidopsis plants are transformed by the floral dip method as described in Bent and Clough, Plant J. 1998 December; 16(6):735-43, which is herein incorporated by reference in its entirety.

[0329]Both wild-type plants (A. thaliana, ecotype Columbia), and plants with a transfer DNA (T-DNA) insertion mutation in the At4g24160 locus are used for transformations. The T-DNA knockout is in an exon of the Arabidopsis homolog of the human CG1-58 gene. For Arabidopsis plants with CGI-58 mutation, there is an increase in cystosolic lipid droplets in leaves when compared to wild-type plants (James e...

example 2

Generation of FSP27 and PLIN2 Expressing Homozygous Transgenic Plants with High Lipid Content

[0334]Seven homozygous lines of FSP27-expressing plants in the cgi58 mutant background, as well as one homozygous line expressing PLIN2 (ADRP) are raised. The new plants are completely viable and healthy with higher lipid accumulation as shown by microscopic data (FIG. 7).

[0335]Seedlings are grown on solidified nutrient medium under selection. Seven Arabidopsis homozygous lines in T2 generation over-expressing the FSP27 in the cgi58 knockout background are identified. Also, one Arabidopsis homozygous line in T2 generation overexpressing the ADRP in the cgi58 knockout background is identified. Lines that are no longer segregating (homozygous) are selected for harvest and extraction. FIG. 7 shows representative confocal images of leaves having preponderance of lipid droplets in both lines as well as the cgi-58 knockout background.

example 3

Identification of Triglyceride-Accumulatory Domain of FSP27

[0336]Using deletion-mutagenesis, the domain of amino acids 120-220 of the mouse FSP27 protein (SEQ ID NO: 2), which is associated with lipid accumulation in adipocytes, is dissected. The domain 120-220 of mouse FSP27 is a core-portion of FSP27 protein. As shown in FIG. 8, adipocytes expressing amino acids 120-220 of the mouse FSP27 protein accumulate lipids faster than adipocytes expressing the full length mouse FSP27 protein.

[0337]The present invention also provides genetically engineered plants expressing only the triglyceride-accumulating domain of FSP27 (such as amino acids 120-220 of mouse FSP27), in order to accumulate lipids / oils at a faster rate than the full length protein. For the plants that need to be harvested from time to time for biofuel production, expressing the triglyceride-accumulating domain can be useful for improving lipid / or production.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Tmaaaaaaaaaa
droplet sizeaaaaaaaaaa
lipid droplet sizeaaaaaaaaaa
Login to view more

Abstract

In some embodiments, the present invention provides a method of elevating lipid content in vegetative (non-seed) plant or algal cells, plant tissues, or whole plants by genetically modifying the plant or algae to express a protein or polypeptide associated with lipid metabolism (such as fat-specific protein 27) of animal origin or plant origin. Also provided are genetically-modified plant or algal cells, plant tissues, or whole plants with elevated cellular lipid content, expressing a protein or polypeptide associated with lipid metabolism (such as fat-specific protein 27) of animal (e.g. human) origin or plant origin.

Description

CROSS-REFERENCE TO RELATED APPLICATION[0001]The present application claims the benefit of U.S. Provisional Application Ser. No. 61 / 739,499, filed Dec. 19, 2012, and U.S. Non-provisional application Ser. No. 13 / 830,012, filed Mar. 14, 2013, both of which are hereby incorporated by reference in their entirety, including any figures, tables, or drawings.BACKGROUND OF THE INVENTION[0002]Plants are a primary source of human and / or animal food, excellent feedstock for fuels, and useful for production of desirable chemicals. Plants synthesize and store lipids, primarily, in cytosolic lipid droplets. In plants, seeds are the primary site of oil synthesis and storage; vegetable oils (such as triacylglycerol) are used as a form of energy during seed germination. Vegetable oils can be synthesized in non-seed (such as leaf) tissues; however, their abundance is low and the stored lipids are presumed to be metabolized rapidly, perhaps for the recycling of fatty acids for energy or the synthesis o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(United States)
IPC IPC(8): C12N15/82C12N9/10C12N9/20C07K14/415
CPCC12N15/8247C07K14/415C12N9/1029C12Y301/01003C12Y203/0102C12Y203/01158C12N9/20C12P7/6463
Inventor PURI, VISHWAJEETCHAPMAN, KENTJAMES, CHRISTOPHERCAI, YINGQI
Owner BOSTON MEDICAL CENTER INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap