49 results about "Lipid extraction" patented technology

The present disclosure provides compositions and methods of recovering a policosanol or a sterol from a mixture comprising one or more fatty alcohols, aldehydes, fatty acids, and / or sterols. The mixture may be, for example, a lipid extract obtained from industrial fermentation processes (e.g., filter cake, distillate residue). The policosanol and / or sterol fraction recovered may be enriched in certain fatty alcohols (e.g., 1-octacosanol, 1-triacontanol, and / or 1-dotriacontanol), or enriched in certain sterols (e.g., ergosterol, campesterol, stigmasterol, and / or β-sitosterol). These bioactive molecules may be useful in the treatment or prevention of various metabolic conditions (e.g., obesity, dyslipidemia, cardiovascular conditions, and other chronic metabolic diseases).

The invention relates to a method for extracting and detecting lipid in liquid raw milk, and provides a method for extracting and detecting lipid in liquid raw milk by verifying and preferably selecting a Bligh-Dyer method for extracting lipid in liquid raw milk and optimizing chromatography and mass spectrometry conditions, so as to improve the separation and identification capability of complex lipid molecules. And the detection sensitivity and stability are obviously improved.

The present disclosure provides compositions and dosage forms thereof, wherein the compositions comprise hyaluronic acid and a lipid extract comprising a ceramide and glycolipid. The present disclosure further provides methods for improving a skin property in a subject by administering the composition or dosage form to the subject.

The invention belongs to the technical field of biological medicines, and particularly discloses a pork liver source / photosensitizer-loaded pork liver source lipid nanoparticle and application thereof in endoplasmic reticulum stress enhanced phototherapy. The lipid extraction method provided by the invention has the advantages of mature process, low cost, easily available raw materials, clear components of the obtained lipid, and high biocompatibility. The pork liver source lipid nanoparticles prepared from natural lipid extracted from pork liver have good endoplasmic reticulum targeting property, and can be used as a carrier to efficiently load a photosensitizer IR780; the related nanoparticle preparation method is simple, good in repeatability and easy for expanded production. A phototherapy system constructed by the nanoparticles can generate active oxygen in situ in an endoplasmic reticulum under near-infrared laser irradiation to initiate strong endoplasmic reticulum stress, so that the anti-tumor effect of photothermal and photodynamic combined treatment is remarkably enhanced, and the nanoparticles can be widely applied to efficient and accurate treatment of various solid tumors.

Composition in the form of a nail polish consisting of: A nail polish base and A lipid extract of the aerial parts of the halophyte plant Crithmum maritimum in a mass proportion of between 0.1% by mass and 2% by mass of said nail polish base and in which the constituent solvent of said lipid extract is a mixture of triglycerides of fatty acids which contain from eight to ten carbon atoms; its use in cosmetics and pharmacy.

The present invention relates to a powder composition consisting essentially of a lipid extract of Serenoa repens also called as Saw palmetto, polyethylene glycol, colloidal silicone dioxide, silicified microcrystalline cellulose and magnesium stearate. The said powder composition has a desirable flow property that provides easy capsule filling. The invention further relates to a process for the preparation of said powder composition and use thereof as medicament in the treatment of benign prostatic hyperplasia in adult men.

The present application relates to a method for extracting lipids from microorganisms, comprising the steps of: (a) preparing a microbial fermentation solution containing lipids; (b) fluidized-bed drying the microbial fermentation solution of step (a) so as to form microbial granules; and (c) press-extracting the microbial granules of step (b), wherein a solvent is not used during lipid extraction.

The invention belongs to the technical field of astaxanthin lipid extraction and waste biomass resource utilization, and particularly relates to a preparation method and application of a hydrophilic crayfish astaxanthin extract. The method comprises the following steps of: crushing and squeezing a raw material which is a by-product of crayfish processing, performing enzymolysis by using papain, squeezing, and finally performing flash extraction by using ethanol to obtain the crayfish astaxanthin extract. In the astaxanthin extract prepared by the method disclosed by the invention, the content of astaxanthin reaches up to 10849.75 mu g / g extract, the maximum content of phospholipid can reach 42.35%, and the astaxanthin extract has good hydrophilicity.

The invention relates to the field of vegetable oil extraction, in particular to a method for extracting rice bran oil from rice bran by an ultrasonic coupling composite enzymolysis technology, and provides a novel method for preparing the rice bran oil. The invention discloses a method for preparing rice bran oil through an ultrasonic coupling enzyme method. The method comprises the steps of mixed rice bran pretreatment, ultrasonic coupling enzymolysis reaction and lipid extraction. Wherein the ultrasonic coupling enzymolysis reaction comprises the following steps: adding water into the rice bran, uniformly mixing, adjusting the pH value of the system, adding quantitative plant extraction enzyme, putting into an ultrasonic instrument, setting specific temperature, ultrasonic power and reaction time for enzymolysis, and finally terminating the reaction for extraction. An ultrasonic coupling enzyme method adopted by the invention is combined with biological enzymolysis and physical ultrasonic action, and a brand new path is opened up for efficiently preparing oil from the rice bran.

Provided herein are workflows to isolate both metabolites and lipids ("2in1"), or metabolites, lipids and proteins ("3in1"). The 2in1 methods comprise treating a sample containing metabolites and lipids with a mixture comprising methanol and ethanol, which precipitates proteins, adding water to increase metabolite recoveries, and passing the treated sample through a solid phase extraction (SPE) matrix which has an affinity for lipids. The flow-through from the SPE matrix contains metabolites, and lipids can be eluted from the SPE matrix with a lipid elution solution. The 3in1 methods are similar to the 2in1, but proteins are pelleted prior to SPE extraction, and it is the pellet that is then analyzed for proteins.

The invention provides a vaccine or a pharmaceutical composition based on a mycobacterium lipid extract as well as preparation and application thereof, and relates to the technical field of biological medicine, the mycobacterium lipid extract is bacterial lipid extracted from mycobacterium thalli (including mycobacterium tuberculosis and nontuberculous mycobacterium) by a common lipid extraction method, and the mycobacterium lipid extract is prepared from the mycobacterium thalli (including mycobacterium tuberculosis and nontuberculous mycobacterium). The mycobacterium lipid extract can be used as a vaccine adjuvant, an immunomodulator, a training immunity inducer, a drug delivery carrier auxiliary component or a targeting ligand, and is suitable for preventing and treating various diseases.

The application discloses a method for relieving obesity cardiomyopathy lipid toxicity by Chemerin and belongs to the technical field of pharmacological research. Mice are selected to establish an obesity mouse model, and are equally divided into a control group, a high-fat diet group, an AAV CMKLR1 virus group, and a high-fat + AAV CMKLR1 virus group. In succession, mouse heart ultrasound, TSE system detection, glucose tolerance test, and insulin tolerance test are carried out, and mouse blood glucose is measured at intervals. Then, tissue protein is extracted for protein immunoblotting, and tissue RNA is extracted for reverse transcription, and then qPCR is used to detect the expression of related genes. After paraffin embedding and slicing of mouse tissues, HE, WGA, immunofluorescence and other pathological staining are carried out. MTBE method is used for lipid extraction and detection of lipid content. ELISA method is used to measure serum Chemerin, and Acyl-RAC method is used to detect protein palmitoylation. Finally, the experimental results are analyzed to draw a conclusion. The present application proves by clinical data and mouse models that Chemerin compensatorily increases in the process of obesity, and Chemerin can relieve cardiac lipid toxicity caused by obesity and protect cardiac function.

The invention relates to a cosmetic composition for improving hair density comprising, as active ingredient, a lipid extract of Coleus forskohlii, a hydrophilic extract of Saxifraga rotundifolia, a lipid extract of Triticum vulgare and a hydrophilic extract of Quillaja saponaria, combined with at least five biomimetic peptides. The invention also relates to a method for the cosmetic treatment of the hair and scalp and a dermatological composition for use as a medicament for treating hair loss.

The present invention relates to an anti-inflammatory composition comprising, as an active ingredient, a lipid extract of Oncorhynchus mykiss eggs. The anti-inflammatory activity of the lipid extract of Oncorhynchus mykiss eggs, of the present invention, was confirmed by analyzing the fatty acid composition and cytotoxicity of the lipid extract of Oncorhynchus mykiss eggs, and the effects thereof on inhibiting NO production, regulating the expression of cytokines, inhibiting NF-κB and MAPK activation, and inhibiting CD86 expression on the surface of immune cells.

Disclosed is a method of lipid extraction for biofuel production comprising treating an ethanol-biomass mixture with ozone and / or at least one protease, followed by extraction of the ethanol fraction with an immiscible organic solvent. Also disclosed is a process for biofuel production comprising culturing psychrophilic and alkaliphilic microalgae, harvesting the microalgae and extracting lipids from the harvested cells. Also disclosed is lipid biofuel from microalgae, characterized by reduced chlorophyll content.

The invention relates to the field of biological medicine, in particular to application of isaria cicadae miquel in preparation of medicine for treating SPTLC1 gene related motor neuron disease, and has the following beneficial effects that (1) a preparation method of a lipid extract of isaria cicadae miquel is optimized, and the content of effective components of the lipid extract is higher; (2) the cordyceps sobolifera is proved to be used for treating the SPTLC1 gene related motor neuron disease on a cellular level and a patient level; in a cell experiment, the isaria cicadae miquel extract can reduce the sphingolipid and deoxysphingolipid levels of an SPTLC1 gene mutation HEK293T cell line, which indicates that the isaria cicadae miquel extract can inhibit the activity of SPT enzyme; in the patient level, the isaria cicadae miquel can remarkably reduce the level of sphingolipid and deoxysphingolipid in the blood of a patient suffering from the SPTLC1 gene mutation related motor neuron disease, it is proved that the isaria cicadae miquel has the capacity of treating the SPTLC1 gene related motor neuron disease, a new reference is provided for developing effective medicine for treating the SPTLC1 gene related motor neuron disease, and the isaria cicadae miquel can be used for preparing a medicine for treating the SPTLC1 gene related motor neuron disease. The sources of clinical medicines are enriched.

The lipid extraction system (100) includes a handle (102), a tube (104), and a solvent (106). The handle (10)2 includes a vacuum system. The tube (104) includes an aperture (108) configured to accept a lipid therethrough. The tube (104) is coupled to the handle (102). The tube (104) is configured to retain the solvent (106) while collecting the lipid from the sample. More specifically, the aperture (108) of the tube (104) retaining the solvent may contact a surface of a sample, thus extracting the lipid of the sample into the solvent (106) within the tube. The lipid collected in the retained solvent (106) may be dispensed in a testing container (110) for analysis, such as a multiple reaction monitoring (MRM) profiling analysis.

The invention belongs to the technical field of traditional Chinese medicine pharmacy, and particularly relates to poecilobdella manillensis freeze-dried powder as well as a preparation method and application thereof. The preparation method of the poecilobdella manillensis freeze-dried powder comprises the following steps: (1) taking living poecilobdella manillensis or freezing and melting the living poecilobdella manillensis, and homogenizing to obtain a poecilobdella manillensis sample solution; (2) carrying out water extraction on the poecilobdella manillensis sample liquid; (3) performing fat extraction on the poecilobdella manillensis precipitate subjected to water extraction; and (4) combining the two extracts to prepare freeze-dried powder. The hirudin content of the poecilobdella manillensis freeze-dried powder prepared by combining a water extraction method with a fat extraction method is remarkably higher than that of a commercially available poecilobdella manillensis product, and the poecilobdella manillensis freeze-dried powder has a remarkable effect on treating thrombotic diseases and other vascular diseases.

The present invention relates to a lipid extract of camellia oleifera oil concentrated into an unsaponifiable fraction, a cosmetic, pharmaceutical or dermatological composition comprising suitable excipients and said lipid extract, and uses thereof.

Aspects of the present disclosure are directed to lipid extraction from insects. According to an aspect of the present disclosure, a method for extracting lipids includes preparing an insect biomass from dried insects (e.g., by grinding them into a powder), incubating the powder with a green solvent under suitable extraction conditions to form a lipid-containing solution and a defatted 5 biomass, separating the lipid-containing solution from the defatted biomass, and removing the solvent to recover the lipid product. The disclosure further includes a composition containing the lipid product obtained by this method, and using the composition as an anti-foaming agent in fermentation or bioprocessing media. In another embodiment, the method also includes adding a non-polar solvent to the lipid-containing solution, adding ice-cold acetone to precipitate phospholipids, and separating the phospholipid precipitate. According to another aspect, the composition containing the phospholipids can be used in nutraceutical, food-grade, pharmaceutical-grade, or cosmetic-grade applications.

The invention relates to a lipid extract of linseed oil Linum usitatissimum, concentrated in its unsaponifiable fraction, its preparation process and a cosmetic, pharmaceutical or dermatological composition comprising a suitable excipient and said lipid extract. The invention also relates to such an extract or such a composition for its use in the prevention or treatment of disorders or pathologies of the skin, mucous membranes or appendages. The invention finally relates to a non-therapeutic cosmetic care process for dry skin, preferably with a feeling of tightness, comprising the topical or oral administration, preferably topical, of such an extract or such a composition.

The invention provides a method for analyzing lipid in a biological sample. The method comprises the processes of extraction of lipid in a biological sample, separation and purification, a high-resolution mass spectrometry detection method and the like, and specifically comprises the following steps: after the lipid in the biological sample is extracted, thin-layer chromatography rapid analysis is carried out, and the type of the contained lipid is determined; determining a silica gel column separation method and steps by combining an analysis result of thin-layer chromatography; and further separating and purifying the lipid by using a silica gel chromatographic column, eluting and separating by using liquid chromatography, and performing high-resolution mass spectrometry detection and analysis. The method further comprises the step of analyzing and processing the detected lipid by using software, and finally determining lipid type information. According to the method, the purity of the lipid and the separation degree of isomers of the lipid can be remarkably improved, a low-abundance lipid signal is improved, the lipid co-outflow behavior is reduced, a high-definition secondary mass spectrum is obtained, the accuracy and coverage rate of qualitative and quantitative analysis of the lipid are effectively improved, and the method is easy to operate, high in repeatability and high in universality.

The invention relates to a lipid extract of linseed oil, Linum usitatissimum, which is concentrated in its unsaponifiable fraction, to the method for preparing same, and to a cosmetic, pharmaceutical or dermatological composition comprising a suitable excipient and the lipid extract. The invention also relates to an extract or a composition of this kind, for use thereof in preventing or treating disorders or diseases of the skin, the mucous membranes or the hair and nails. Finally, the invention relates to a method for the non-therapeutic cosmetic care of dry skin, preferably skin which feels tight, comprising the topical or oral administration, preferably topical administration, of an extract or a composition of this kind.

This invention provides a pretreatment device for detecting OPO functional oils in dairy products. The pretreatment device includes an oil extraction module and a reagent loading module. The oil extraction module includes a total lipid extraction device, a functional oil separation device, an intermediate sample storage device, and a solid-phase extraction device. These devices are connected by a piping assembly equipped with valves. The reagent loading module includes an autosampler and a reagent storage device. The autosampler draws reagents and adds them to the functional oil separation device or the solid-phase extraction device. This invention enables fully automated total lipid extraction, separation of OPO and other functional oils, and removal of interfering substances from dairy products. It solves the problems of complex, time-consuming, inaccurate, and unsafe manual pretreatment of samples for detecting OPO and other functional oils in dairy products.

A composition in the form of a nail varnish, consisting of: a nail varnish base and a lipid extract of the aerial parts of the halophyte plant Crithmum maritimum in a mass proportion of between 0.1% by mass and 2% by mass of said nail varnish base, wherein the component solvent for said lipid extract is a mixture of fatty acid triglycerides which comprise from eight to ten carbon atoms; use thereof in cosmetics and pharmaceuticals.

The present disclosure relates to a system and method for the preparation and clarifying of biological tissue samples. The system comprises a sample subsystem equipped with one or more containers for holding samples, alongside a reagent subsystem containing one or more containers for various specialized reagents. These reagents comprise a Refractive Index Reagent (RIR), preferably 2,2'-thiodiethanol (TDE), and preferably also comprises a Lipid Extraction Reagent (LER) and / or a Labelling Reagent (LR), more preferably further comprises one or more of a Bleaching Reagent (BR), a Calcium Removal Reagent (CRR), and a Fixative Reagent (FR). The sample and reagent subsystems are interconnected through one or more multi-channel subsystems, facilitating efficient fluid communication. Additionally, the system preferably integrates a waste disposal unit, enhancing operational convenience and safety. The method for sample clarifying comprises a bleaching step which comprises contacting the sample with hydrogen peroxide (H2O2), and / or a clarifying step that comprises contacting the sample with 2,2'-thiodiethanol (TDE).

Portable Microfluidic Laboratory Device for Solvent-Free Lipid Extraction from Yeast

The invention discloses a method for extracting cyperus esculentus lipid by combining supercritical carbon dioxide with a subcritical dimethyl ether mixture, and belongs to the technical field of lipid extraction. The method comprises the following steps: S1, mixing supercritical carbon dioxide with subcritical dimethyl ether to obtain an extraction system; s2, mixing the cyperus esculentus powder with an extraction system, putting the mixture into a high-pressure extraction kettle for extraction, slowly reducing the pressure after the extraction is finished, collecting lipid through a separator, and refining to obtain the cyperus esculentus lipid. According to the method, a new green, environment-friendly, efficient and synergistic lipid extraction technology is realized by constructing a supercritical CO2 and subcritical DME mixed solvent system, and the selected CO2 and DME solvents are non-toxic, volatile and free of harmful residues, so that the safety and cleanliness of the product are ensured, and the overall extraction efficiency and effect are improved.

This invention belongs to the field of medical dressing technology, specifically relating to a medical dressing for wounds and its preparation method. The medical dressing for wounds of this invention, by weight percentage, consists of the following raw materials: 6-12% polyvinyl alcohol, 3-5% carboxymethyl chitosan, 0.1-0.3% lipid extract, 0.5-1% curculigoside, 0.1-0.5% glycerin, 0.01-0.05% vitamin E, with the balance being deionized water. The addition of lipid extract and curculigoside to the medical dressing for wounds of this invention, when used in combination, can improve the dressing's exudate absorption capacity and significantly improve the healing quality of the wound. The porous carrier added to the lipid extract not only enhances the antioxidant activity of the lipid extract but also facilitates the diffusion and absorption of wound exudate, provides a scaffold for cell adhesion and proliferation, promotes epidermal cell and blood vessel regeneration, and accelerates the wound healing process.

A composition in the form of a nail varnish, consisting of: a nail varnish base and a lipid extract of the gametophytes of the brown alga Undaria pinnatifida in a mass proportion of between 0.1% by mass and 2% by mass of said nail varnish base, wherein the component solvent for said lipid extract is a mixture of fatty acid triglycerides, the acyl chains of which comprise from eight to ten carbon atoms; use thereof in cosmetics and pharmaceuticals.