81 results about "Lipid extraction" patented technology

Methods, systems, and apparatuses for extracting non-polar lipids from microalgae are achieved using a lipid extraction device having an anode and a cathode that forms a channel and defines a fluid flow path through which an aqueous slurry is passed. An electromotive force is applied across the channel at a gap distance in a range from 0.5 mm to 200 mm to cause the non-polar lipids to be released from the algae cells. The non-polar lipids can be extracted at a high throughput rate and with low concentrations of polar lipids such as phospholipids and chlorophyll.

Microorganisms such as microalgae are collected and separated from a host medium such as water. Cellular walls and membranes of the microorganisms are then ruptured to release their lipids using a lipid extraction unit. Thereafter, the lipids from the host medium are collected and separated using a lipid collection and separation unit. Related apparatus, systems, techniques and articles are also described.

Embodiments of the present invention concern methods, compositions, and apparatus for the continuous conversion of algal lipids into biodiesel. In some embodiments, the biodiesel is formed in a multi-step sequence, the first steps occurring in the presence of water and a strong acid wherein the lipids are released from the algae by means of mechanical and chemical action and are then hydrolyzed to free fatty acids. In a subsequent step, this free fatty acid mixture is reacted with methanol to generate fatty acid methyl esters (also known as biodiesel). Such methods produce biodiesel from algal lipids without the requirement for separate algal cell lysis or lipid extraction or purification prior to the acid catalysis sequence. In other embodiments, the multi-step acid catalysis sequence occurs at 100° C. at two atmospheres of pressure.

A method and device for aggregating algae in an aqueous solution is disclosed. The method can include providing an algae feed comprising a liquid and algae dispersed therein. The algae feed can be aggregated by applying a nanosecond pulsed electric field to the algae feed. The nanosecond pulsed electric field can include a plurality of electric pulses having a pulse duration ranging from 1 to 1,000 nanoseconds. The method can also include separating an aggregated algae stream from the algae feed and feeding the aggregated algae stream to a lipid extraction operation.

The invention provides a method for enriching lipid in Antarctic krill, which comprises the following steps: (1) preprocessing the Antarctic krill serving as a raw material; (2) leaching the Antarctic krill obtained in the step (1) with a solvent to obtain a leaching liquid; (3) concentrating the leaching liquid obtained in the step (2) to a certain volume; (4) refining the concentrated leaching liquid obtained in the step (3); and (5) performing vacuum concentrating on the refined leaching liquid obtained in the step (4) until the residual volume of the solvent is 0.1% lower than the volume of the solvent used in the step (2) so as to obtain the lipid of Antarctic krill. According to the invention, the Antarctic krill or head capsule of Antarctic krill is preferentially used as a raw material, the lipid extraction efficiency is increased through the preprocessing, solvent leaching and refining methods, the lipid quality is improved, the comprehensive utilization rate of Antarctic krill is remarkably increased, the product quality is improved, and the obtained lipid in Antarctic krill is applied to healthcare foods.

A method for determination of at least one of the lipid species in a biological sample comprising subjecting the sample to lipid extraction to obtain a lipid extract and subjecting the resulting lipid extract to multidimensional electrospray ionization mass spectrometry using either precursor ion or neutral loss scanning (or both) of all naturally occurring aliphatic chains, lipid fragments and precursor ions leading to observed fragments to generate a multidimensional matrix whose contour densities provides structural and quantitative information directly without chromatography. A method for determination of lipid content and / or lipid molecular species composition and quantity directly from lipid extracts of a biological sample comprising subjecting said lipid extract to electrospray ionization multidimensional mass spectrometry by comparisons to standards and algorithms described herein.

The invention provides a method for joint production of biological ethanol and microbial lipid by using methyl cellulose as raw material and then realizing the joint production of ethanol and biodiesel by using the methyl cellulose as the raw material. The method comprises the following steps of: (1) ethanol fermentation is carried out for a cellulose solid obtained after the methyl cellulose is pretreated; (2) a product of hemicellulose hydrolyzation in the pretreatment process is used for microbial lipid fermentation; (3) waste liquor produced after ethanol in ethanol fermentation is recycled and waste liquor produced after microbial lipid is fermented are used for microbial lipid fermentation; and (4) yeast fermented by alcohol and thallus residue produced after lipid extraction are hydrolyzed and then are used as a nitrogen source to be used for ethanol fermentation and microbial fermentation process. The method provided by the invention can convert the methyl cellulose with low grade into two biomass fuels of bioethanol and biodiesel and simultaneously can reduce waste water emission in ethanol production.

The invention provides a comprehensive utilization method for preparing biodiesel and glycerine by using microalgae lipid as a raw material. The method integrates microalgae lipid extraction technology, ester exchange reaction technology, technology for producing biodiesel with high purity and high performance and technology for effectively reclaiming byproduct glycerine, and provides a method for completely preparing the biodiesel by using the microalgae lipid as the raw material. The method has the characteristics of simple flow, high efficiency, low cost, high product quality, low ambient pressure and the like, is easy to realize in the industry, and has good industrial application value.

The invention relates to an aquatic product producing area tracing method based on fatty acid carbon stable isotopes. The method includes the steps that A, preprocessing is performed, wherein collected aquatic products are processed, completely frozen, dried and ground into powder; B, total lipid extraction is performed, wherein 5.0 g of samples are taken, a chloroform and methanol solution is added, digestion stays overnight after oscillating extraction, filtering is performed, an extraction solution is washed with a sodium chloride solution, standing is performed so that layering can be achieved, a chloroform layer is collected, and after drying performed through anhydrous sodium sulfate, sample total lipid is acquired by means of concentration of a nitrogen blowing method; C, methyl esterification of fatty acid is performed, wherein a sulfuric acid-methyl alcohol methyl esterification method is adopted, a sulfuric acid-methyl alcohol solution is added into the total lipid, methyl esterification is performed in a water bath, n-hexane is added for oscillation after cooling, standing is performed so that layering can be achieved, supernatant is taken and placed in a bottle to be refrigerated and stored; D, the fatty acid C stable isotopes are measured, wherein fatty acid methyl ester samples are analyzed and identified through a gas chromatograph mass spectrometer; E, a result is analyzed, wherein by comparing fatty acid in different sea area samples, aquatic products in different sea areas and different varieties are identified.

Methods, systems, and apparatuses for extracting non-polar lipids from microalgae are achieved using a lipid extraction device having an anode and a cathode that forms a channel and defines a fluid flow path through which an aqueous slurry is passed. An electromotive force is applied across the channel at a gap distance in a range from 0.5 mm to 200 mm to cause the non-polar lipids to be released from the algae cells. The non-polar lipids can be extracted at a high throughput rate and with low concentrations of polar lipids such as phospholipids and chlorophyll.

The invention discloses a method for detecting lipid molecules in salmon. The method includes the following steps that firstly, a sample is processed in advance, wherein salmon meat is cleaned and ground into meal, an organic solvent I is added into the salmon meal to be shaken and evenly mixed, ultrasonic ice bath extraction is conducted, pure water is added after extraction to be shaken and evenly mixed, the obtained mixture is centrifuged by a freezing centrifugal machine, a bottom-phase solution is obtained, dried at low temperature and dissolved again by means of an organic solvent II, and a lipid crude extract is obtained; secondly, enrichment and purification are conducted, wherein a solid-phase extraction column is activated, then the lipid crude extract is subjected to sample loading, spraying and elution, and the collected elution solution is a lipid extraction solution; thirdly, the lipid extraction solution is subjected to mass spectrometric detection and analysis. By means of the method, structure verification and quantitative analysis of phosphatidylcholine (PC) and phosphatidyl ethanolamine (PE) in salmon can be achieved.

The invention provides a food lipid extraction method and a detection method of lipid in food. The food lipid extraction method comprises the following steps of: mixing and centrifuging a food sample with ultrapure water, methanol and dichloromethane for multiple times, wherein the lower layer of an organic phase obtained after multiple times of centrifugation is lipid. A small amount of samples is utilized, and extracts of different lipids through one-time treatment by using a safer lipid extraction reagent are obtained, and are subjected to qualitative and quantitative detection simultaneously or respectively. The sample consumption is reduced, the safety is improved, the consumables in the sample pretreatment and detection process are saved, the cost is reduced, and the efficiency is improved.

Systems and methods for removing lipids from a fluid, such as plasma, or from lipid-containing organisms. A fluid is combined with at least one extraction solvent, which causes the lipids to separate from the fluid or from lipid-containing organisms. The separated lipids are removed from the fluid. The extraction solvent is removed from the fluid or at least reduced to an acceptable concentration enabling the delipidated fluid to be administered to a patient without the patient experiencing undesirable consequences. Once the fluid has been processed, the fluid may be administered to a patient who donated the fluid, to a different patient, or stored for later use.

A one-step process for the lysis of microalgae cell walls and separation of the cellular lipids for use in biofuel production by utilizing a hydrophilic ionic liquid, 1-butyl-3-methylimidazolium. The hydrophilic ionic liquid both lyses the microalgae cell walls and forms two immiscible layers, one of which consists of the lipid contents of the lysed cells. After mixture of the hydrophilic ionic liquid with a suspension of microalgae cells, gravity causes a hydrophobic lipid phase to move to a top phase where it is removed from the mixture and purified. The hydrophilic ionic liquid is recycled to lyse new microalgae suspensions.

The present invention is concerned with a method of identifying microbial strains (e.g. from a cell culture), the method comprising; i) a lipid extraction step, comprising extraction of phospholipids from the microbe, suitably with an extraction composition comprising more than 50 vol % MeOH; ii) a sample preparation step, comprising preparation of a MALDI sample incorporating the extracted lipids; iii) a data gathering step, comprising performing MALDI-based mass spectrometry on the MALDI sample, and iv) a microbe identification step, comprising analysis of the mass spectrometry data to characterise or identify the microbial strain. Suitably the method also includes extracting proteins from the microbes and analysing the extracted proteins using MALDI-based mass spectrometry so as to obtain not only lipid m / z data but also protein m / z data.

The invention provides a process for performing high pressure combined ultrasound-associated extraction on active substance from mushroom. The process sequentially comprises the following step: performing material preprocessing, pulverization, low-temperature lipid extraction, filter slag pressurization, ultrasound extraction and ethanol extraction, and finally drying to obtain the active substance. The method is characterized in that lipid extraction is adopted, the active substance dissolved with lipid is extracted, slag subjected to the lipid extraction adopts a high pressure combined ultrasound extraction technology to ensure that the difference between the internal pressure and the external pressure of the raw materials is changed to result in the change of a cell structure; then, an ultrasound-assisted extraction technology is adopted, an ultrasound cavatition function is utilized, target components are easily dissociated from an extracted matter, so that the active substance can be quickly and effectively extracted from the organism, thus greatly increasing the yield; then, the active substances subjected to lipid extraction and ethanol extraction are mixed and dried to prepare the active substance, and the active substance is stored at low temperature and the extraction rate of the active substance is greatly increased.

Methods of lipid extraction from biomass, in particular wet algae, through conventionally heated subcritical water, and microwave-assisted subcritical water. In one embodiment, fatty acid methyl esters from solids in a polar phase are further extracted to increase biofuel production.

The present invention provides an extraction method of effective ingredients in maca. The method is characterized by consisting of the following steps: maca powder at 150-250 meshes and water at a mass ratio of 1:(5-20) are mixed, the mixture is subjected to a high-pressure homogenization, the mixture is sprayed into a vacuum container to be soaked via a high-pressure nozzle, soaking liquid is subjected to a membrane filtration, filtrate is concentrated at a temperature of 10-40 DEG C, 40-50% of water content is removed to obtain extraction liquid containing maca water-soluble effective components, residue and anhydrous ethanol are mixed at a mass ratio of 1:(5-10) evenly, the mixture is soaked for 2-5 hours, the soaking liquid is subjected to the membrane filtration, the filtrate is concentrated at a temperature of 10-40 DEG C, and 70-80% of the ethanol is removed to obtain the extract containing maca lipid-soluble effective components. On the basis of the water extraction, then the lipid extraction is conducted, so that both the water-soluble and lipid-soluble effective components are extracted and the extraction rate of the maca effective components is high. All the technologies are conducted at room temperature to effectively prevent the destructions of the activities of the effective ingredients.

The invention relates to microbial analysis. The present invention is concerned with a method of identifying microbial strains (e.g. from a cell culture), the method comprising; i) a lipid extraction step, comprising extraction of phospholipids from the microbe, suitably with an extraction composition comprising more than 50vol% MeOH; ii) a sample preparation step, comprising preparation of a MALDI sample incorporating the extracted lipids; iii) a data gathering step, comprising performing MALDI-based mass spectrometry on the MALDI sample, and iv) a microbe identification step, comprising analysis of the mass spectrometry data to characterise or identify the microbial strain. Suitably the method also includes extracting proteins from the microbes and analysing the extracted proteins using MALDI-based mass spectrometry so as to obtain not only lipid m / z data but also protein m / z data.

The invention relates to a method for preparing abalone viscera phospholipid through HPEF (High Pulsed Electric Field)-coupled biological enzymolysis and belongs to the field of deep processing of aquatic food. The method comprises the following preparation steps in sequence: raw material preprocessing, PEF-coupled biological enzymolysis, lipid extraction, phosphatide separation based on silicagel column chromatography, protein extraction, alcohol extraction of polysaccharide and the like, thereby sequentially obtaining phosphatide, neutral fat, protein, polysaccharide, pigment and the like in abalone. The method is simple in operating process and low in cost; the method is capable of performing deep processing and comprehensive utilization on byproducts-viscera generated by processing in the Chinese abalone industry, and serving as intermediate feed stock, the prepared phosphatide, protein, polysaccharide, pigment and the like of abalone can be applied to various fields such as foods and medicine. The technology is applicable to industrial production of various scales, and is capable of effectively solving waste discharge of the abalone production and processing industry.

According to one embodiment, the disclosure provides a system for algal cell lysis. The system may include at least one algal cell, a plurality of metal nanoparticles, and an electromagnetic radiation generator. The generator may be able to generate radio frequency or microwave radiation that excites the plurality of metal nanoparticles, resulting in lysis of the algal cell. The disclosure also provides a system for recovery of a lipid from an algal cell similar to the above system but also including a separator. The disclosure further provides a method of recovering a lipid from an algal cell by supplying a plurality of metal nanoparticles to at least one algal cell, exciting the plurality of metal nanoparticles with radio frequency or microwave electromagnetic radiation, lysing the algal cell to release the lipid, and separating the lipid from the lysed algal cell.

A method for determination of at least one of the triglyceride molecular species in a biological sample comprising the subjecting sample to lipid extraction to obtain a lipid extract and subjecting the resulting lipid extract to 2D electrospray ionization tandem mass spectrometry (ESI / MS / MS) with neutral loss scanning of all naturally occurring aliphatic chains and contour analysis of 2D intercept peaks. A method for determination of tricylglyceride content and / or TG molecular species directly from a lipid extract of a biological sample comprising subjecting said lipid extract to electrospray ionization tandem mass spectrometry.

A method for determination of at least one of the lipid species in a biological sample comprising subjecting the sample to lipid extraction to obtain a lipid extract and subjecting the resulting lipid extract to multidimensional electrospray ionization mass spectrometry using either precursor ion or neutral loss scanning (or both) of all naturally occurring aliphatic chains, lipid fragments and precursor ions leading to observed fragments to generate a multidimensional matrix whose contour densities provides structural and quantitative information directly without chromatography. A method for determination of lipid content and / or lipid molecular species composition and quantity directly from lipid extracts of a biological sample comprising subjecting said lipid extract to electrospray ionization multidimensional mass spectrometry by comparisons to standards and algorithms described herein.

The present invention provides a lipid extraction device comprising a capillary tube, wherein the capillary tube comprises a first region containing a first filler; and a second region present in a region other than the first region and containing a second filler having polarity different from the first filler. The present invention also provides a system and a method for extracting and analyzing lipids from a biological sample using the lipid extraction device.