23 results about "Lipid extraction" patented technology

The invention belongs to the technical field of biological medicines, and particularly discloses a pork liver source / photosensitizer-loaded pork liver source lipid nanoparticle and application thereof in endoplasmic reticulum stress enhanced phototherapy. The lipid extraction method provided by the invention has the advantages of mature process, low cost, easily available raw materials, clear components of the obtained lipid, and high biocompatibility. The pork liver source lipid nanoparticles prepared from natural lipid extracted from pork liver have good endoplasmic reticulum targeting property, and can be used as a carrier to efficiently load a photosensitizer IR780; the related nanoparticle preparation method is simple, good in repeatability and easy for expanded production. A phototherapy system constructed by the nanoparticles can generate active oxygen in situ in an endoplasmic reticulum under near-infrared laser irradiation to initiate strong endoplasmic reticulum stress, so that the anti-tumor effect of photothermal and photodynamic combined treatment is remarkably enhanced, and the nanoparticles can be widely applied to efficient and accurate treatment of various solid tumors.

The present application relates to a method for extracting lipids from microorganisms, comprising the steps of: (a) preparing a microbial fermentation solution containing lipids; (b) fluidized-bed drying the microbial fermentation solution of step (a) so as to form microbial granules; and (c) press-extracting the microbial granules of step (b), wherein a solvent is not used during lipid extraction.

The invention belongs to the technical field of astaxanthin lipid extraction and waste biomass resource utilization, and particularly relates to a preparation method and application of a hydrophilic crayfish astaxanthin extract. The method comprises the following steps of: crushing and squeezing a raw material which is a by-product of crayfish processing, performing enzymolysis by using papain, squeezing, and finally performing flash extraction by using ethanol to obtain the crayfish astaxanthin extract. In the astaxanthin extract prepared by the method disclosed by the invention, the content of astaxanthin reaches up to 10849.75 mu g / g extract, the maximum content of phospholipid can reach 42.35%, and the astaxanthin extract has good hydrophilicity.

The invention relates to the field of vegetable oil extraction, in particular to a method for extracting rice bran oil from rice bran by an ultrasonic coupling composite enzymolysis technology, and provides a novel method for preparing the rice bran oil. The invention discloses a method for preparing rice bran oil through an ultrasonic coupling enzyme method. The method comprises the steps of mixed rice bran pretreatment, ultrasonic coupling enzymolysis reaction and lipid extraction. Wherein the ultrasonic coupling enzymolysis reaction comprises the following steps: adding water into the rice bran, uniformly mixing, adjusting the pH value of the system, adding quantitative plant extraction enzyme, putting into an ultrasonic instrument, setting specific temperature, ultrasonic power and reaction time for enzymolysis, and finally terminating the reaction for extraction. An ultrasonic coupling enzyme method adopted by the invention is combined with biological enzymolysis and physical ultrasonic action, and a brand new path is opened up for efficiently preparing oil from the rice bran.

The invention provides a vaccine or a pharmaceutical composition based on a mycobacterium lipid extract as well as preparation and application thereof, and relates to the technical field of biological medicine, the mycobacterium lipid extract is bacterial lipid extracted from mycobacterium thalli (including mycobacterium tuberculosis and nontuberculous mycobacterium) by a common lipid extraction method, and the mycobacterium lipid extract is prepared from the mycobacterium thalli (including mycobacterium tuberculosis and nontuberculous mycobacterium). The mycobacterium lipid extract can be used as a vaccine adjuvant, an immunomodulator, a training immunity inducer, a drug delivery carrier auxiliary component or a targeting ligand, and is suitable for preventing and treating various diseases.

The application discloses a method for relieving obesity cardiomyopathy lipid toxicity by Chemerin and belongs to the technical field of pharmacological research. Mice are selected to establish an obesity mouse model, and are equally divided into a control group, a high-fat diet group, an AAV CMKLR1 virus group, and a high-fat + AAV CMKLR1 virus group. In succession, mouse heart ultrasound, TSE system detection, glucose tolerance test, and insulin tolerance test are carried out, and mouse blood glucose is measured at intervals. Then, tissue protein is extracted for protein immunoblotting, and tissue RNA is extracted for reverse transcription, and then qPCR is used to detect the expression of related genes. After paraffin embedding and slicing of mouse tissues, HE, WGA, immunofluorescence and other pathological staining are carried out. MTBE method is used for lipid extraction and detection of lipid content. ELISA method is used to measure serum Chemerin, and Acyl-RAC method is used to detect protein palmitoylation. Finally, the experimental results are analyzed to draw a conclusion. The present application proves by clinical data and mouse models that Chemerin compensatorily increases in the process of obesity, and Chemerin can relieve cardiac lipid toxicity caused by obesity and protect cardiac function.

Disclosed is a method of lipid extraction for biofuel production comprising treating an ethanol-biomass mixture with ozone and / or at least one protease, followed by extraction of the ethanol fraction with an immiscible organic solvent. Also disclosed is a process for biofuel production comprising culturing psychrophilic and alkaliphilic microalgae, harvesting the microalgae and extracting lipids from the harvested cells. Also disclosed is lipid biofuel from microalgae, characterized by reduced chlorophyll content.

The invention belongs to the technical field of traditional Chinese medicine pharmacy, and particularly relates to poecilobdella manillensis freeze-dried powder as well as a preparation method and application thereof. The preparation method of the poecilobdella manillensis freeze-dried powder comprises the following steps: (1) taking living poecilobdella manillensis or freezing and melting the living poecilobdella manillensis, and homogenizing to obtain a poecilobdella manillensis sample solution; (2) carrying out water extraction on the poecilobdella manillensis sample liquid; (3) performing fat extraction on the poecilobdella manillensis precipitate subjected to water extraction; and (4) combining the two extracts to prepare freeze-dried powder. The hirudin content of the poecilobdella manillensis freeze-dried powder prepared by combining a water extraction method with a fat extraction method is remarkably higher than that of a commercially available poecilobdella manillensis product, and the poecilobdella manillensis freeze-dried powder has a remarkable effect on treating thrombotic diseases and other vascular diseases.

Aspects of the present disclosure are directed to lipid extraction from insects. According to an aspect of the present disclosure, a method for extracting lipids includes preparing an insect biomass from dried insects (e.g., by grinding them into a powder), incubating the powder with a green solvent under suitable extraction conditions to form a lipid-containing solution and a defatted 5 biomass, separating the lipid-containing solution from the defatted biomass, and removing the solvent to recover the lipid product. The disclosure further includes a composition containing the lipid product obtained by this method, and using the composition as an anti-foaming agent in fermentation or bioprocessing media. In another embodiment, the method also includes adding a non-polar solvent to the lipid-containing solution, adding ice-cold acetone to precipitate phospholipids, and separating the phospholipid precipitate. According to another aspect, the composition containing the phospholipids can be used in nutraceutical, food-grade, pharmaceutical-grade, or cosmetic-grade applications.

This invention provides a pretreatment device for detecting OPO functional oils in dairy products. The pretreatment device includes an oil extraction module and a reagent loading module. The oil extraction module includes a total lipid extraction device, a functional oil separation device, an intermediate sample storage device, and a solid-phase extraction device. These devices are connected by a piping assembly equipped with valves. The reagent loading module includes an autosampler and a reagent storage device. The autosampler draws reagents and adds them to the functional oil separation device or the solid-phase extraction device. This invention enables fully automated total lipid extraction, separation of OPO and other functional oils, and removal of interfering substances from dairy products. It solves the problems of complex, time-consuming, inaccurate, and unsafe manual pretreatment of samples for detecting OPO and other functional oils in dairy products.

The invention discloses a method for extracting cyperus esculentus lipid by combining supercritical carbon dioxide with a subcritical dimethyl ether mixture, and belongs to the technical field of lipid extraction. The method comprises the following steps: S1, mixing supercritical carbon dioxide with subcritical dimethyl ether to obtain an extraction system; s2, mixing the cyperus esculentus powder with an extraction system, putting the mixture into a high-pressure extraction kettle for extraction, slowly reducing the pressure after the extraction is finished, collecting lipid through a separator, and refining to obtain the cyperus esculentus lipid. According to the method, a new green, environment-friendly, efficient and synergistic lipid extraction technology is realized by constructing a supercritical CO2 and subcritical DME mixed solvent system, and the selected CO2 and DME solvents are non-toxic, volatile and free of harmful residues, so that the safety and cleanliness of the product are ensured, and the overall extraction efficiency and effect are improved.

The invention relates to the field of biological medicine, in particular to a preparation method of recombinant lipid nanoparticles based on soybean meal, which comprises the following steps: pretreating a soybean meal raw material, increasing the lipid extraction rate, determining an extraction process step, extracting lipid from soybean meal, and optimizing specific process parameters for extracting lipid from soybean meal by adopting a response surface method and Box-Behnken design. The method comprises the following steps: extracting soybean meal lipid, preparing reconstructed lipid nanoparticles from the extracted soybean meal lipid, culturing and analyzing various cells, quantifying the effect of the reconstructed lipid nanoparticles, evaluating the drug loading capacity, optimizing the drug loading capacity and the ratio of the encapsulation efficiency to the drug loading, and evaluating the biological membrane correlation by utilizing the prior art. According to the invention, the reconstructed lipid nanoparticles derived from soybean meal are developed, drug delivery is carried out, a lipid extraction process is optimized, the reconstructed lipid nanoparticles enhance the cell uptake ability and show a remarkable synergistic effect on the carried drug, and drug waste can be reduced and the curative effect can be ensured by evaluating the drug carrying ability of the reconstructed lipid nanoparticles; and the administration dosage is optimized.

This invention provides a method for extracting membrane-constituting lipids using reagents with lower biotoxicity, while maintaining their activity more stably, and for efficiently preparing lipid membrane vesicles. [Solution] (A) A method for producing a lipid extract, comprising the step of contacting a biological sample containing membrane-constituting lipids with an extraction solvent containing a dihydric alcohol to extract the membrane-constituting lipids.

The invention discloses a traditional Chinese medicine essential oil composition for treating alopecia, which comprises traditional Chinese medicine essential oil and a deep eutectic solvent, or the traditional Chinese medicine essential oil composition is a water-based traditional Chinese medicine essential oil transdermal delivery system prepared by dropwise adding the traditional Chinese medicine essential oil into the deep eutectic solvent, uniformly mixing and then uniformly mixing with water. The traditional Chinese medicine essential oil composition is simple in preparation process, can stably entrap essential oil, enhances percutaneous penetration of the essential oil through lipid extraction, effectively delivers the essential oil to hair follicles to play a role in promoting hair regeneration activity, has the effect of treating alopecia, especially androgen-derived alopecia, and has a good application prospect. The adverse reaction caused by applying medicines such as finasteride and minoxidil is avoided. The invention discloses application of the traditional Chinese medicine essential oil composition in preparation of a medicine with hair regeneration promoting activity, preferably application in preparation of a medicine for treating alopecia, and more preferably application in preparation of a medicine for treating androgen-derived alopecia.

The present invention relates to a collar for non-human animals, preferably for companion animals, comprising a polymeric matrix and a lipid extract comprising sphingomyelins. It also refers to the collar for use as a medicine, particularly for use as an adjuvant and for use in the treatment or prevention of atopic dermatitis or allergic dermatitis, as well as in restoring the integrity of the skin during or after an atopic dermatitis or an allergic dermatitis, increasing the hydration and flexibility of the skin, facilitating skin regeneration or reducing itching of the skin.

The invention relates to a detection reagent for lipid biomarkers, application and a screening method. The detection reagent comprises a lipid extraction protein precipitation reagent, a redissolving reagent and a chromatographic separation reagent, the lipid extraction protein precipitation reagent is a mixture of methyl tert-butyl ether and methanol in a ratio of 5: 1, and the redissolving reagent is methanol and dichloromethane in a ratio of 1: 1. Injecting the detection reagent into a detection device comprising a liquid chromatography-tandem mass spectrometer, and detecting through liquid chromatography-tandem mass spectrometry to obtain the content of each lipid in the lipid biomarker. The FH neonatal marker is represented through lipidomics characteristics for the first time, the lipid biomarker is screened out and used for evaluating the disease severity of the FH neonatal, and the obtained lipid biomarker has high value for early screening and timely intervention of FH. The lipid biomarker obtained through screening has good biological stability and representativeness, the biological activity of the lipid biomarker can be guaranteed after long-time storage, and the reliability of a detection result can be guaranteed.

This document provides workflows for separating metabolites and lipids (“2in1”) or separating metabolites, lipids, and proteins (“3in1”). The 2in1 method involves: treating a sample containing metabolites and lipids with a mixture of methanol and ethanol to precipitate proteins; adding water to improve metabolite recovery; and passing the treated sample through a lipid-affinity solid-phase extraction (SPE) matrix. The effluent from the SPE matrix contains metabolites, and lipids can be eluted from the SPE matrix using a lipid elution solution. The 3in1 method is similar to the 2in1 method, except that proteins are granulated prior to SPE extraction, and then the granules are analyzed for protein content.

The present invention relates to methods of lipid extraction, and specifically provides improved methods for extracting and preparing lipids for use in pharmaceuticals, nutraceuticals and functional food products from biological sources.

A protein preparation may be obtained from dried lipid extracted marine algae. The novel hydrolysate has high solubility and digestibility, low ash content, and high protein content (measured as g amino acid / g of product), with an essential amino acid profile that makes an excellent protein supplement for human consumption. The process utilizes a hydrolyzation reaction at high solids content to minimize water footprint and ash content, while increasing its solubility and digestibility.

The invention discloses a method integrating microalgae lipid accumulation promotion, algae cell flocculation and lipid extraction, and belongs to the field of bioengineering and energy. An AIE group is used as a main body structure, positive charges and hydrophobic chains are modified through quaternization, so that AIE molecules can be combined with algae cells through electrostatic interaction and hydrophobic interaction, and lipid accumulation of the algae cells is promoted by improving the light energy utilization rate of the algae cells. Furthermore, integration from lipid synthesis to extraction is realized based on the positive charge and active oxygen generation performance of molecules.

The invention relates to a process for producing an oil, in particular an edible oil, having a high content of unsaturated fatty acids from a microalgae preparation. According to the invention, the microalgae preparation is treated to form an intermediate product, and the intermediate product is subjected to a substance separation for separating off impurities, with triglycerides being used as a carrier for the impurities. As a microalgae preparation, advantageously a microalgae oil and / or a lipid extract is used. The microalgae preparation is preferably obtained from microalgae, in particular photoautotrophic and / or mixotrophic microalgae. In one embodiment of the invention, the microalgae preparation is treated by transesterification to form the intermediate product, wherein the microalgae preparation is preferably converted into ethyl esters. In one embodiment of the invention, the organic phase of the intermediate product is separated off from the inorganic phase, and the organic phase of the intermediate product is subjected to a substance separation for separating off impurities, with triglycerides being used.

A cosmetic composition for improving hair density comprising, as active ingredients, a lipid extract of Coleus forskolhii, a hydrophilic extract of Saxifraga rotundifolia, a lipid extract of Triticum vulgare, and a hydrophilic extract of Quillaja saponaria, combined with at least five biomimetic peptides. A process for the cosmetic treatment of hair and scalp and a dermatological composition for its use as a medicinal product for treating hair loss.

The invention belongs to the technical field of pork lipidome extraction and analysis, and discloses a pork lipidome analysis method based on liquid chromatography-tandem mass spectrometry, and the method comprises the following steps: S1, taking pig adipose tissue, and carrying out low-temperature grinding and refining treatment; s2, carrying out lipid extraction by adopting a two-phase solvent system; s3, performing centrifugal separation on the extracting solution, and collecting an upper-layer organic phase; s4, concentrating and drying the organic phase, and re-dissolving the organic phase in the chromatographic compatible solvent; and S5, carrying out separation and structure identification on the redissolved lipid by using an electron activation dissociation fragmentation liquid chromatography-tandem mass spectrometry system. A two-phase solvent system is adopted to replace chloroform, so that the risk of highly toxic solvent exposure is eliminated while the lipid recovery rate is guaranteed; an electron activation dissociation technology is integrated, chemical bonds are directionally broken through electron free radicals, and fine structure identification of pig characteristic lipid is achieved; and a support is provided for efficient extraction and fine analysis of the pig lipidome.