The invention relates to an aquatic product producing area tracing method based on fatty acid carbon stable isotopes. The method includes the steps that A, preprocessing is performed, wherein collected aquatic products are processed, completely frozen, dried and ground into powder; B, total lipid extraction is performed, wherein 5.0 g of samples are taken, a chloroform and methanol solution is added, digestion stays overnight after oscillating extraction, filtering is performed, an extraction solution is washed with a sodium chloride solution, standing is performed so that layering can be achieved, a chloroform layer is collected, and after drying performed through anhydrous sodium sulfate, sample total lipid is acquired by means of concentration of a nitrogen blowing method; C, methyl esterification of fatty acid is performed, wherein a sulfuric acid-methyl alcohol methyl esterification method is adopted, a sulfuric acid-methyl alcohol solution is added into the total lipid, methyl esterification is performed in a water bath, n-hexane is added for oscillation after cooling, standing is performed so that layering can be achieved, supernatant is taken and placed in a bottle to be refrigerated and stored; D, the fatty acid C stable isotopes are measured, wherein fatty acid methyl ester samples are analyzed and identified through a gas chromatograph mass spectrometer; E, a result is analyzed, wherein by comparing fatty acid in different sea area samples, aquatic products in different sea areas and different varieties are identified.