Aquatic product producing area tracing method based on fatty acid carbon stable isotopes

Abstract

The invention relates to an aquatic product producing area tracing method based on fatty acid carbon stable isotopes. The method includes the steps that A, preprocessing is performed, wherein collected aquatic products are processed, completely frozen, dried and ground into powder; B, total lipid extraction is performed, wherein 5.0 g of samples are taken, a chloroform and methanol solution is added, digestion stays overnight after oscillating extraction, filtering is performed, an extraction solution is washed with a sodium chloride solution, standing is performed so that layering can be achieved, a chloroform layer is collected, and after drying performed through anhydrous sodium sulfate, sample total lipid is acquired by means of concentration of a nitrogen blowing method; C, methyl esterification of fatty acid is performed, wherein a sulfuric acid-methyl alcohol methyl esterification method is adopted, a sulfuric acid-methyl alcohol solution is added into the total lipid, methyl esterification is performed in a water bath, n-hexane is added for oscillation after cooling, standing is performed so that layering can be achieved, supernatant is taken and placed in a bottle to be refrigerated and stored; D, the fatty acid C stable isotopes are measured, wherein fatty acid methyl ester samples are analyzed and identified through a gas chromatograph mass spectrometer; E, a result is analyzed, wherein by comparing fatty acid in different sea area samples, aquatic products in different sea areas and different varieties are identified.

Description

technical field [0001] The invention relates to a method for tracing the origin of aquatic products based on fatty acid carbon stable isotopes, which belongs to the field of food safety and is mainly used for identifying and tracing the real origin of aquatic products. Background technique [0002] my country is a big aquaculture country, and its aquaculture production accounts for more than 80% of the world's total production, ranking first in the world. However, food safety issues have been hindering the development and upgrading of the industry. The construction of the aquatic product quality traceability system will help solve the problem of adulteration and sale of aquatic products, and can effectively protect the vital interests of aquatic product manufacturers, farmers and consumers. In recent years, my country has successively implemented identification management in the quality traceability of aquatic products from breeding, processing to circulation, etc., and app...

AI Technical Summary

Problems solved by technology

However, food safety issues have been hindering the development and upgrading of the industry

Organisms can synthesize fatty acids, modify carbon chain length and introduce double bonds, but the transformation ability is different, usually with the increase of biological level, the ability gradually decreases, and vertebrates almost lose this ability

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