309 results about "Fatty acid methyl ester" patented technology

The invention discloses an anti-blocking agent and a preparation method and application thereof, and belongs to the technical field of chemical fertilizer assistants, and the anti-blocking agent comprises at least one of a surfactant capable of controlling the length of hydrophilic and hydrophobic chain segments, a macromolecular surfactant and inert powder. Wherein the surfactant capable of controlling the length of the hydrophilic and hydrophobic chain segment comprises at least one of fatty alcohol-polyoxyethylene ether, fatty acid methyl ester polyoxyethylene ether, fatty amine polyoxyethylene ether, fatty acid amide and fatty glyceride. The anti-blocking agent is prepared in a blending mode, the anti-blocking agent is directly put into the liquid raw materials of the fertilizer to be evenly mixed, through the spray drying technology, moisture is completely evaporated, main components of the anti-blocking agent are firmly attached to the surfaces of powder fertilizer particles, the raw materials are easy to obtain, preparation is convenient, and the anti-blocking agent has the advantages of being high in anti-blocking efficiency, convenient to use and the like.

The invention discloses industrial-grade mixed oil produced by utilizing kitchen waste oil and a preparation method of the industrial-grade mixed oil, and belongs to the technical field of recycling of the kitchen waste oil. The invention relates to industrial-grade mixed oil produced by using kitchen waste oil and a preparation method thereof, and the preparation method comprises the following steps: firstly collecting kitchen waste containing the waste oil, and storing the kitchen waste in a container through special equipment; carrying out screen solid-liquid separation, cleaning grease on the surface of the solid waste, and then carrying out precipitation separation; then carrying out centrifugal separation, chemical cleaning, water washing, acid treatment, dehydration, degumming and metal ion removal on the separated grease and oil-containing liquid; carrying out ultrasonic-assisted multistage ester exchange after enzymolysis optimization, carrying out membrane separation on glycerol and fatty acid methyl ester, and carrying out reduced pressure distillation to obtain a high-purity fraction; carrying out adsorption refining, plasma treatment and supercritical fluid extraction; finally, nano particles are added and blended with additives, efficient recycling of the kitchen waste oil is achieved, and then high-purity industrial-grade mixed oil is prepared.

This application discloses a detergent tablet free of SLS surfactant and its preparation method. The detergent tablet includes a film-forming agent, an anionic surfactant, a non-ionic surfactant, a chelating agent, an enzyme preparation, an auxiliary agent and a essence. The film-forming agent includes one or more of polyvinyl alcohol, cellulose, and dodecenyl succinate starch ester. The anionic surfactant includes at least two of sodium α-olefin sulfonate, sodium fatty acid methyl ester sulfonate, and sodium hydroxyethyl sulfonate. The non-ionic surfactant includes at least two of fatty alcohol polyoxyethylene ethers, fatty acid methyl ester ethoxylate, and oil ethoxylate.

The utility model discloses reaction equipment with high crude methyl ester yield, which comprises a stirring kettle and a material circulating system, the material circulating system comprises a circulating pump, a spray pipe arranged in the stirring kettle and a mixing nozzle arranged on the spray pipe, and a bottom outlet of the stirring kettle is connected with a feed port of the circulating pump through a first pipeline. An outlet of the circulating pump is connected with the spray pipe through a second pipeline; the mixing nozzle comprises a first sprayer and a second sprayer, and an outer flaring in the lower portion of the second sprayer is in clearance fit with the conical head through a connecting piece. The material circulating system can realize bottom-out and top-in of materials, so that the mixing of an oil phase and an enzyme water phase is promoted through the reciprocating circulation. In addition, the material circulating system is provided with a plurality of mixing nozzles, the flow speed of spraying of the first spray head is large, so that a negative pressure suction gap is formed at the joint of the second spray head and the first spray head, materials in the stirring kettle can enter the mixing nozzles under the action of negative pressure, an oil phase and an enzyme water phase are intensively mixed, and the yield of fatty acid methyl ester is improved.

The invention discloses a multi-solid waste interface collaborative optimization regenerant and a preparation method of a regenerated asphalt mixture thereof, and belongs to the technical field of road material regeneration. In order to solve the problems that a traditional single regenerant is insufficient in performance improvement, low in solid waste utilization rate and high in carbon emission, citric acid is used for activating steel slag powder to enhance the reactivity of the steel slag powder and fatty acid methyl ester (FAME) ester groups, and a silane coupling agent is used for modifying waste tire rubber powder to improve the interfacial compatibility of the waste tire rubber powder with steel slag and asphalt; lignosulfonate stabilizes FAME, reduces interfacial tension of FAME and improves dispersibility of FAME, and then three-component interface collaborative optimization is achieved. According to the invention, the rigid reinforcing effect of the steel slag powder, the elastic recovery characteristic of the rubber powder and the regeneration function of the FAME are fully utilized, and a gradient temperature mixing process is combined to form a'rigid reinforcing-elastic buffering-dynamic regeneration 'synergistic mechanism. The composite regenerant can recover the viscosity, rheology and other characteristics of the aged asphalt, improve the high and low temperature and other comprehensive pavement performance of the regenerated asphalt mixture, and achieve the purposes of multi-element solid waste recycling and low carbonization.

The invention relates to the technical field of thermal insulation materials, in particular to a low-smoke high-flame-retardant rubber and plastic thermal insulation material for locomotives and a preparation process thereof.The low-smoke high-flame-retardant rubber and plastic thermal insulation material for locomotives is prepared from a rubber base material, a flame retardant, a foaming agent, an accelerant, a composite smoke suppressor, an anti-aging agent and a plasticizer, the flame retardant is epoxy fatty acid methyl ester grafted medium-chain chlorinated paraffin; the flame retardant and the composite smoke suppressor are compounded to achieve a synergistic effect, so that the flame retardance of the material can be remarkably improved, the smoke concentration of the material during combustion can be reduced, the flame retardance of the material can be improved, the flame retardance of the material can be improved, the flame retardance of the material can be improved, and the flame retardance of the material can be improved. The foaming agent, the accelerant, the anti-aging agent and the plasticizer are compounded, so that the stability and the elasticity of the material can be further enhanced, and the material has a wide application prospect.

The invention relates to the technical field of harmlessness and resource utilization of hazardous wastes, and provides a cold-rolled oil sludge resource treatment method which utilizes the characteristics of low hydrogen bond degree, low dielectric constant and strong reactivity of methanol in a supercritical state. The method comprises the following steps: carrying out esterification reaction on oil-water, oil-solid, oil-water-solid and other interface fatty acids in the cold-rolled oil sludge and methanol to generate fatty acid methyl ester, and dissolving the fatty acid methyl ester in the methanol by utilizing the strong solubility of the supercritical methanol; the method comprises the following steps: firstly, separating iron filings in the cold-rolled oil sludge, so that the interface acting forces of oil-water, oil-solid, oil-water-solid and the like in the cold-rolled oil sludge are reduced, the interface structure is damaged and liquid drops are gathered, a liquid phase consisting of high-grade iron filings and oil-water can be obtained through simple solid-liquid separation, and then oil-water separation is realized through extraction, so that the oil-water-solid three-phase separation in the cold-rolled oil sludge is realized.

The present invention discloses a lipase mutant tolerant to organic solvents, its encoding gene and application. The present invention screens and obtains a lipase mutant G266C with significantly improved organic solvent tolerance and higher specific activity by performing site-directed mutagenesis on wild-type Rhizopus oryzae lipase. Experiments show that after incubation for 30 min in methanol, ethanol, isopropanol and acetone at a final concentration of 50% (v / v), the residual enzyme activity of the lipase mutant G266C is 3.1, 2.9, 2.1 and 2.6 times that of the wild-type Rhizopus oryzae lipase, respectively. The organic solvent tolerance is significantly improved, and the enzyme activity is better than that of the wild-type, and it can be applied to the synthesis of biodiesel such as fatty acid methyl esters, and is more suitable for application in the industrial field.

The present application provides novel polypeptides with lipase activity, which comprise the amino acid sequences shown in SEQ ID NO: 4 or 6, or sequences obtained by substituting, deleting or adding at least one amino acid to the above sequences. The present application also provides polynucleotides encoding the polypeptides, expression vectors containing the above polynucleotides and host cells. In addition, the present application also relates to the uses of the polypeptides with lipase activity. The polypeptides disclosed herein can rapidly prepare fatty acid methyl esters at high temperatures and high methanol concentrations, and are thus used for the preparation of biodiesel.

The invention discloses a hand-care detergent as well as a preparation method and application thereof. The hand-care detergent comprises the following components: sodium fatty acid methyl ester sulfonate, sodium linear alkyl benzene sulfonate and sodium alcohol ether sulphate. The formula system of the hand-care detergent is in a low-irritation state, and the hand-care effect is achieved while the decontamination effect of the detergent is guaranteed.

The invention discloses a metal degreasing agent and a preparation method thereof, and belongs to the technical field of degreasing. Comprising lipase, protease, short-chain fatty acid methyl ester ethoxylate sodium sulfonate, cocamidopropyl betaine, alkyl glucoside, sodium silicate, sodium metasilicate, sodium citrate, disodium ethylene diamine tetraacetate, calcium formate, polyvinyl alcohol-sodium alginate microcapsules, a polyoxyethylene polyoxypropylene block copolymer, sodium carboxymethyl cellulose and water. And the polyvinyl alcohol-sodium alginate microcapsule exists in the metal degreasing agent as a carrier of the lipase and the protease. According to the metal degreasing agent, a microcapsule protection structure is used in the metal degreasing agent, the two technical problems of enzyme-surfactant compatibility and enzyme-alkaline environment compatibility which are difficult to solve for a long time in an industrial degreasing agent are solved, the degreasing agent is easy to actually operate, special equipment is not needed, a traditional degreasing agent can be directly replaced, and the metal degreasing agent is suitable for industrial application. Good market application prospects are realized.

The invention relates to the technical field of fire extinguishing agents, and particularly discloses a lithium battery fire extinguishing agent and a preparation method thereof. The degradable surfactant is selected from at least one of alkyl glycoside and fatty acid methyl ester sulfonate; the particle size of the nanoscale silicon dioxide particles is 10 to 100 nm; the composite flame retardant is formed by compounding an organic phosphorus compound and graphene oxide according to a mass ratio of 1: (0.5-2); the antifreeze agent is selected from at least one of ethylene glycol and propylene glycol; the thickening agent is selected from at least one of xanthan gum and sodium carboxymethyl cellulose; the preservative is selected from at least one of sodium benzoate and potassium sorbate; through the synergistic effect of the composite flame retardant, the fire extinguishing efficiency is remarkably improved, reburning is inhibited, in addition, nano silicon dioxide is uniformly dispersed after being subjected to surface modification to form a compact heat insulation layer, the degradable surfactant and the environment-friendly preservative are combined, and the agent is free of toxic residues.

The invention relates to a mining underground hydrogen sulfide removal dust falling agent and a preparation method thereof, and belongs to the technical field of dust suppression and dust falling. The dust settling agent provided by the invention comprises the following components: 10-15 parts of fatty acid methyl ester sodium sulfonate; 3 to 5 parts of sodium lauryl sulfate; 4 to 6 parts of sodium dodecyl benzene sulfonate; 3 to 5 parts of dodecyl amine; 1-3 parts of sodium bicarbonate; according to the present invention, through the synergistic interaction effect of the surfactant, the dust particles and the liquid are well dispersed and wetted, and the reaction of the sodium bicarbonate and the hydrogen sulfide is promoted so as to increase the dust adsorption efficiency and the hydrogen sulfide gas neutralization effect, such that the technical purpose of the present invention is achieved. The raw materials are easy to obtain, the preparation process is simple and convenient, the cost is low, the effect is remarkable, the working environment of coal miners can be effectively improved, and safety and health of the miners are guaranteed.

The invention relates to the technical field of cleaning agents, and particularly discloses a composite cleaning agent as well as a preparation method and application thereof. The composite cleaning agent comprises the following raw material components in percentage by mass: 5%-10% of inorganic particles, 5%-15% of a surfactant, 15%-30% of a cleaning aid and the balance of water, wherein the surface active agent is prepared from rhamnolipid, fatty acid methyl ester ethoxylate, cocamidopropyl betaine and sophorolipid; the inorganic particles comprise soluble sodium salt and nano silicon dioxide; the cleaning auxiliary agent comprises a geranium extracting solution, a herba portulacae extracting solution, lauric acid and glycerol. The composite cleaning agent is simple in raw material components, simple and convenient in preparation method, low in cost and free of secondary pollution to the environment. The composite cleaning agent effectively solves the problems that an existing train cleaning agent is poor in cleaning effect, complex in manufacturing process and unfriendly to the environment.

The invention discloses a solvent type cleaning agent for replacing dichloromethane, which is characterized by comprising the following components in parts by weight: 70-90 parts of hydrocarbon solvent D40; 0 to 15 parts of chlorinated methoxy fatty acid methyl ester; and 0-15 parts of butanediol diacetate, wherein the number of parts of the chloro-methoxy fatty acid methyl ester or butanediol diacetate is not 0 at the same time. Compared with the existing dichloromethane cleaning agent, the cleaning agent disclosed by the invention has the advantages of low toxicity, low volatilization amount, no corrosivity, low VOC (Volatile Organic Compounds), certain anti-rust capability and no key control pollutant CFC113, carbon tetrachloride and trichloroethylene.

The invention relates to the technical field of concrete materials, and particularly discloses recycled concrete containing light rubber and a preparation method of the recycled concrete. The recycled concrete containing the light rubber comprises the following components in parts by mass: 100 parts of water; 194 to 198 parts of cement; 34 to 36 parts of fly ash; 375 to 380 parts of fine aggregate; 595 to 600 parts of coarse aggregate; 160 to 170 parts of modified light rubber powder; 2.8 to 3.2 parts of an auxiliary agent; the preparation method of the modified light rubber powder comprises the following steps: 1) crushing and screening waste tires to obtain light rubber powder; 2) adding the light rubber powder into the modification liquid for modification, filtering out solids after modification is completed, and drying to obtain modified light rubber powder; the modified liquid is a compound of water, sodium dodecyl diphenyl ether disulfonate, sodium fatty acid methyl ester sulfonate and aminopropyltriethoxysilane. The method has the advantage that the strength and the crack resistance of the concrete doped with the rubber powder are further improved.

The present invention relates to an agrochemical emulsifiable composition containing: an agrochemical component having an octanol-water partition coefficient (LogPow) of 4.0 or more as an agrochemical active ingredient (A); at least one selected from the group consisting of a fatty acid methyl ester (b1), a fatty acid amide (b2), and an aromatic hydrocarbon solvent (b3) as a solvent (B); and a combination of an anionic surfactant (c1) and a nonionic surfactant (c2) as an emulsifier (C). The agrochemical emulsifiable composition is a composition that has excellent storage stability in an emulsifiable formulation using a poorly water-soluble agrochemical active ingredient, and can exhibit a stable emulsified state and sufficient insecticidal efficacy when prepared into a spray emulsion by mixing with water.

The invention provides an efficient oil-removing water purifying agent and a preparation method thereof. The oil-removing water purifying agent mainly comprises a micro-crosslinking multi-branched cationic copolymer, fatty acid methyl ester ethoxylate, dodecyl dimethyl benzyl ammonium chloride and deionized water. The micro-crosslinked multi-branched cationic copolymer is mainly prepared from a non-polar solvent, a branched monomer, a crosslinking monomer, acrylamide, a cationic monomer, an emulsifier, an initiator, an organic solvent and deionized water, and the fatty acid methyl ester ethoxylate is mainly prepared from methyl stearate, a catalyst, ethylene oxide, isopropanol and deionized water. The efficient oil-removing water purifying agent mainly aims at the defects of an existing oil-removing water purifying agent, the micro-crosslinking multi-branched cationic copolymer is used as a flocculating agent, the fatty acid methyl ester ethoxylate is used as a nonionic surfactant, and through the synergistic effect of the micro-crosslinking multi-branched cationic copolymer and the fatty acid methyl ester ethoxylate, the efficient oil-removing water purifying agent is obtained. Therefore, the prepared oil-removing water purifying agent has better oil-removing effect and dispersing performance.

The invention relates to the field of washing products, in particular to a whitening and decontaminating laundry sheet and a preparation method thereof. The whitening and decontaminating laundry sheet comprises a film-forming agent, an anionic surfactant, a nonionic surfactant, a fluorescent whitening agent, an anti-fouling agent, an enzyme preparation, a plant extract, a chelating agent and an auxiliary agent, the film-forming agent comprises a film-forming agent A and a film-forming agent B; the film-forming agent A is polyvinyl alcohol, and the film-forming agent B comprises one or more of polyvinylpyrrolidone and modified polyvinyl alcohol; the anionic surfactant comprises sodium alkyl sulfate, sodium fatty acid methyl ester sulfonate and sodium alpha-olefin sulfonate; the nonionic surfactant is prepared from polyglycerol fatty acid ester, fatty alcohol-polyoxyethylene ether, fatty acid methyl ester ethoxylate and alkyl glucoside. The whitening and decontaminating laundry sheet is high in dissolvability, has a remarkable whitening effect on fabrics, has decontaminating power, and has the advantages of being convenient to carry and use, free of solid matter residues after being used and the like.

The invention provides a method for measuring the content of fatty acid in sorghum. The method is efficient, easy to operate and capable of measuring various fatty acids. The method comprises the steps that S10, sorghum is smashed and sieved, and smashed sorghum is obtained; s20, taking the crushed sorghum according to a first preset weight to obtain a crushed sorghum sample to be detected, adding a sulfuric acid-methanol solution into the crushed sorghum sample, carrying out water bath reflux, and after the reflux is finished, centrifuging and taking supernate; and S30, adding a potassium hydroxide-methanol solution to the supernatant, and carrying out a heat-preservation reaction for a first preset duration in a water bath to obtain a reaction product liquid containing fatty acid methyl ester. And S40, adding an extraction solvent into the reaction product liquid to extract fatty acid methyl ester, oscillating and ultrasonically extracting for a second preset time to obtain an extracting solution containing fatty acid methyl ester. And S50, carrying out qualitative and quantitative determination on fatty acid methyl ester in the extracting solution by adopting a gas chromatograph to obtain a fatty acid methyl ester determination result, and calculating the content of various fatty acids in the crushed sorghum sample according to the fatty acid methyl ester determination result and a corresponding fatty acid content conversion calculation relation.

The invention relates to the field of inorganic nonmetal powder materials, and discloses a preparation method of silicon nitride powder, which comprises the following steps: (1) mixing a surfactant, liquid ammonia and an organic solvent to form a to-be-reacted solution, and mixing the to-be-reacted solution with a silicon tetrachloride solution for substitution reaction to obtain a solution containing silicon imine suspended matters; the surfactant is selected from one or more of sodium dodecyl sulfate, polyvinylpyrrolidone, sodium stearate, sodium dodecyl benzene sulfonate, fatty acid methyl ester ethoxylate sulfonate, fatty acid sorbitan, polysorbate-20 and polysorbate-80; the surfactant is selected from one or more of sodium dodecyl sulfate, polyvinylpyrrolidone, sodium stearate, sodium dodecyl benzene sulfonate, fatty acid methyl ester ethoxylate sulfonate, fatty acid sorbitan, polysorbate-20 and polysorbate-80; (2) carrying out suspended matter extraction and impurity removal on the solution containing the silicon imine suspended matter to obtain silicon imine powder; (3) performing thermal decomposition on the silicon imine powder to obtain amorphous silicon nitride powder; and (4) crystallizing the amorphous silicon nitride powder to obtain the alpha-phase silicon nitride powder. The method can reduce the impurity content and agglomeration degree of the precursor so as to improve the quality of the silicon nitride powder.

The invention relates to the technical field of adhesives, in particular to clean plate glue and a preparation method thereof.The clean plate glue comprises a main agent and a curing agent, and the main agent at least comprises, by weight, 40-60 parts of hydroxyl compounds, 4-9 parts of ester plasticizers, 30-50 parts of filler and 2-4 parts of auxiliaries; the hydroxyl compound at least comprises castor oil, polyether polyol and micromolecular polyol; the hydroxyl value of the polyether polyol is 300 to 400 mgKOH / g; the micromolecular polyol is propylene glycol; the mass ratio of the micromolecular polyol to the ester plasticizer is (0.5-1.5): (4-6); the ester plasticizer is fatty acid methyl ester, has a relatively long active period and open time under a high-temperature condition (more than 35 DEG C) in summer, meets the bonding requirements and processing requirements of a surface material and a core material in the clean board, and has a very high market application value.

The invention belongs to the technical field of fuel oil, and particularly relates to hydrogen energy engine fuel oil taking coal-to-oil as a base material and a preparation method of the hydrogen energy engine fuel oil. Comprising the following components: 35-45 parts of coal-based Fischer-Tropsch synthesis crude liquid wax, 5-15 parts of white oil, 2-8 parts of heavy wax, 15-25 parts of hydrogenated anthracene oil, 2-8 parts of fatty acid methyl ester, 15-25 parts of hydrogenated coal diesel oil and 1-5 parts of an additive. The preparation method comprises the following steps: S1, weighing the raw materials according to the proportion of the fuel oil of the hydrogen energy engine; and S2, adding the raw materials into a stirring container, and uniformly stirring to obtain the hydrogen energy engine fuel oil. The fuel oil does not belong to dangerous chemicals, the ignition performance of the fuel oil is superior to that of current national 6 diesel oil, the terminal sale price of the fuel oil is lower than that of the diesel oil, and market requirements are met.

A method of equipment decontamination may include: introducing a cleaning stream comprising hydrogen and a solvent comprising a fatty acid methyl ester and an oxygenated solvent, or alternatively comprising a carrier fluid and a hydrocarbon solvent, into the equipment; and introducing a stream comprising nitrogen into the equipment, wherein the equipment comprises deposits and other contaminants.

This invention discloses a method for preparing high-quality bio-based 1,9-nonanediol, comprising the following steps: transesterification, precipitation, fatty acid methyl ester refining, ozone oxidation pyrolysis, solvent recovery, esterification, dimethyl azelaate refining, and hydrogenation to obtain high-quality bio-based 1,9-nonanediol. This invention achieves gradient and full-component utilization of vegetable oil raw materials: the oil-based portion of triglycerides is used to produce high-quality bio-based 1,9-nonanediol, while simultaneously producing byproducts such as methyl palmitate, methyl stearate, propionic acid, hexanoic acid, and nonanoic acid; the glycerol-based portion of triglycerides is used as a byproduct of glycerol production. This invention utilizes esterification to reconstruct the products of ozone oxidation pyrolysis, converting the high-boiling-point monomethyl azelaate into the low-boiling-point dimethyl azelaate, achieving efficient separation from saturated fatty acid methyl esters. The dimethyl azelaate, as a raw material, is then hydrogenated to obtain high-quality nonanediol, significantly reducing production costs.

This invention relates to an alcohol-based fuel additive, its preparation method, and its application, belonging to the technical field of diesel fuel additives. The additive, by weight, comprises: a mixture of C3-C10 fatty alcohols, fatty acid methyl esters, methyl tert-butyl ether, isoamyl nitrate, dimethyl carbonate, and diesel fuel. The mass ratio of methyl tert-butyl ether to dimethyl carbonate is [value missing]. This invention employs a stepped feeding process, solving the problem of combustion improver failure caused by high shear heat generation by cooling the fuel before adding isoamyl nitrate, and ensuring long-term fuel stability at -10°C using a specific polarity gradient ratio. The fuel prepared by this invention has a high cetane number, strong power, and good anti-stratification effect, making it suitable for compression ignition engines.

The present application relates to a kind of directly oiling oil agent of crude oil of polyester high-speed spinning special oil and preparation method, the oil agent of the present application contains not less than 20wt% anti-coking agent;Anti-coking agent is composed of fatty acid methyl ester ethoxylate type surfactant and polyethylene glycol laurate of double end-capped;Preparation method is: proportionally sequentially adding each component into reaction kettle, stirring uniformly, after 1~1.5h under 40~45 ℃, send to precipitation kettle and carry out cooling, after 15min, filter, directly oiling oil agent of polyester high-speed spinning special oil of crude oil is obtained.The oil agent of the present application contains little water, is not easy to delamination, corruption, not easy to evaporate when passing through hot roll, to avoid the environmental hazards and influence to human health generated, and it has good anti-coking property and antistatic property, the energy consumption when carrying out polyester high-speed spinning also has some reduction.

The invention provides dewaxing, repairing and blocking integrated liquid and application thereof in a pathological specimen immunohistochemical operation method. The dewaxing, repairing and blocking integrated liquid is prepared from the following components in percentage by mass: 1.0 to 5.0 percent of Triton-100, 1.0 to 5.0 percent of Tween 20, 0.1 to 3.0 percent of fatty acid methyl ester ethoxylate, 0.1 to 1.0 percent of tetrahydrofurfuryl alcohol, 0.001 to 0.01 percent of 2-methyl-3-isothiazolinone, 0.01 to 4.0 percent of buffer solution and the balance of water. In the process of applying the dewaxing, repairing and blocking integrated body fluid to the pathological specimen immunohistochemical operation method, compared with an existing laboratory method, the dewaxing, repairing and blocking integrated body fluid is used at the temperature of 65-75 DEG C, the steps of xylene dewaxing and gradient alcohol hydration after dewaxing in an existing laboratory can be replaced, the temperature continues to rise to 95-99 DEG C, and when antigen repairing is conducted on a sample, the temperature of the sample is lowered to 95-99 DEG C; according to the method, the step of blocking 0.3-3% of hydrogen peroxide in an existing laboratory is replaced by the enzyme activity of high temperature on endogenous catalase, and the same effects of dewaxing, repairing and blocking in the existing laboratory are achieved on the basis of reducing the experimental steps and the experimental time.

The invention discloses a high-alkali-resistance textile degreaser as well as a preparation method and application thereof, and relates to the technical field of textile pretreatment. The method comprises the following steps: S1, carrying out a neutralization reaction on fatty acid methyl ester sulfonic acid and a mixed alkali solution of sodium hydroxide and alkanolamine, and controlling the pH value of a reaction endpoint to be 6.0-7.5 to obtain a mixed salt of sodium fatty acid methyl ester sulfonic acid and alkanolamine; s2, adding an alkyl polyglucoside aqueous solution into the mixed salt obtained in the step S1 to form mixed micelles; s3, fatty acid ester ethoxylate and a bio-based cosolvent are added into the mixed micelle obtained in the step S2, homogenization treatment is conducted, and homogeneous microemulsion is obtained; and S4, cooling the microemulsion obtained in the step S3, adding a chelating dispersant and water, and uniformly mixing to form the high-alkali-resistance textile degreaser. The textile degreaser prepared through dication modification and micelle recombination has good alkali resistance, excellent low-temperature water solubility and full-bio-based environmental protection characteristics, and is suitable for pretreatment of textiles such as cotton, polyester and the like.

This invention relates to the field of coal washing and beneficiation technology, and discloses a method for preparing a nano-flotation reagent for coarse coal slime. The method involves mixing sulfonated fatty acid methyl ester with kerosene and n-octanol to form an oil phase, then further mixing this with an aqueous phase containing a nonionic emulsifier, and adding functionalized nano-silica to obtain a mixed solution. This mixed solution is then sequentially treated by ultrasonic dispersion, high-speed stirring, and micro-electrolysis to generate nano / microbubbles in situ and maintain their uniform distribution, resulting in a nano-microemulsion. This invention can significantly improve the adhesion efficiency and loading capacity of coarse coal surfaces, effectively suppress tailings runoff, and improve the transportation and collection efficiency of coarse coal. It solves the problems of low clean coal yield and tailings runoff caused by the low adsorption efficiency and poor foam stability of traditional flotation reagents for coarse coal.