The invention relates to a nematoid-killing composition containing abamectin and paecilomyces lilacinus, which is characterized by comprising abamectin and paecilomyces lilacinus; 100g of nematoid-killing composition is prepared by the following components of 0.1-5g of abamectin, 0.1-1 billion live spores/g of paecilomyces lilacinus, 1-10g of stabilizing agent, 1-90g of solvent and 10-90g of assistant, wherein the assistant is any one or the combination of any two of attapulgite, kaoline, sepiolite, light calcium and kieselguhr and is prepared into a granular formulation or suspending agent or wettable powder; the stabilizing agent is any one or the combination of any two of castor oil, soybean oil, epoxidized soybean oil, propylene glycol, methyl oleate, ethylene glycol, butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA); and the solvent is any one of dimethylbenzene, trimethylbenzene and dimethylformamide. The nematoid-killing composition is used for controlling the knot nematode and has better control effect on the knot nematode.