66 results about "Sorangium cellulosum" patented technology

The invention discloses a fermentation production method based on epothilone B metabolic pathways and belongs to the technical field of fermentation engineering. The method comprises the following steps of: 1) inoculating Sorangium cellulosum into an M26 culture medium, carrying out shake cultivation to obtain a seed solution; 2) inoculating the seed solution into fermentation liquor containing resin, carrying out shake culturing for 72-120 hours at 25-35 DEG C, adding a precursor and small molecular substances into the fermentation liquor containing resin; and 3) filtering the fermentation liquor after the culture is finished, collecting the resin, washing the resin and oscillating and extracting with methyl alcohol to obtain extract liquor containing the epothilone B. According to the invention, the precursor and small molecular substances related to the biosynthetic pathways obtained by screening are added into the fermentation liquor, and the constructive metabolism pathways of the epothilone B are disturbed, so that the excellent bacterial strain gives full play to the ability of anabolism and the fermentation level of the epothilone B is improved greatly, thus the fermentation cost is reduced and the commercial process of the anti-cancer drug is promoted.

The invention relates to a xylose isomerase and the coded protein and the application thereof, pertaining to the technical field of enzyme genetic engineering. A coded xylose isomerase DNA sequence is obtained from mutation at partial positions of a xylose isomerase gene xylA(Genebank EU643621) in Sorangium cellulosum 157-2. The invention also relates to enzyme coded by the DNA sequence and the application of the coded enzyme in the fields of chemical industry, food and medicine. In addition, compared with the traditional xylose isomerase, the activity of xylose isomerase of the invention in saccharomyces cerevisiae is increased by 2.8 times.

Methods are provided for purification of epothilone D and epothilone D analogs from epothilone producing cells such as, for example, Sorangium cellulosum and Myxococcus xanthus (recombinant). Epothilone can be purified from fermentation broth, for example by using resins, chromatography, and crystallization. Epothilone D can be crystallized from a binary solvent system in which water is the forcing solvent.

The invention discloses an alkali proof Sorangium cellulosum strain named Sorangium cellulosum So0157-2, which is preserved in China Center for Type Culture Collection (CCTCC) with a preservation number of CCTCC No. M208078 on May 27, 2008. The invention also discloses the application of the alkali proof Sorangium cellulosum to the preparation of Epothilones. The Epothilones generated by the fermentation of the alkali proof Sorangium cellulosum has high yield stable fermenting property of strains and short fermentation period, is not easy to cause bacteria contaminants and does not generate Epothilones C, D, E, F and other homologues, thus greatly reducing the difficulty in subsequent separation and purification and being an Epothilones producing strain with high value in research and development.

The invention discloses kelp microcrystalline cellulose and a preparation method thereof as well as application of the prepared microcrystalline cellulose. The preparation method of the kelp microcrystalline cellulose comprises the following steps of by adopting waste kelp residues after kelp glue-extracting as the materials, carrying out flocculation collecting, acidification decalcification, alkaline treatment, bleaching treatment, washing, drying and hydrolytic treatment, wherein in the hydrolytic treatment, two different low-temperature cellulases are respectively added according to the addition of 20U/g-30U/g; carrying out enzymolysis for 0.5-12 hours in a water bath of 10 DEG C-40 DEG C; and drying and crushing to obtain the kelp microcrystalline cellulose. According to the preparation method of the kelp microcrystalline cellulose, the production cost is low, the environment pollution is small, the hydrolysis step adopts a double-enzyme jointed enzymolysis technology, the degradation speed is stable, the degraded cellulose is uniform in molecular weight, the cellulose crystalline grains are small and uniform, degradability of the fibers can be effectively controlled, and the microcrystalline cellulose with high purity and high activity is obtained. The kelp microcrystalline cellulose disclosed by the invention is high in cellulose content, high in water holding capacity and expansion force, and can be used for food additive and pharmaceutical adjuvant in the food industry.

The present invention discloses a method for raising secondary metallic product output of myxobacteria, i,e, a method for raising output of metabolic product epothilone A of sorangium cellulosum of ephothilone in myxobacteria, and the said method incldues: 1). naturalization method of lump-grown myxobacterial liquid uniform growth; (2). selecting potato starch and hydrolyzed lactoprotein, etc. suitable for production of epothilone A; and 3). addition method of mixed resin for integrally-eliminating product metabolic accumulation so as to make the output of ephothiloue A product be up to 62.7 mg/L level.

The invention provides a process for producing Epothilones by utilizing Sorangium cellulosum, which comprises improving fermentation culture medium of Sorangium cellulosum, and charging cyclodextrins or cyclodextrin derivatives into the fermentation culture medium. The process can realize higher output of Epothilones production.

The invention discloses a sorangium cellulosum fermentation medium, which comprises a carbon source, a nitrogen source, inorganic salts, a macroporous absorption resin, and water. The fermentation medium also comprises amino acid. When the fermentation medium is used for preparing the epothilone B by fermentation culture of sorangium cellulosum, the performance of strains is stable, the growth is rapid, and the yield of the product epothilone B is obviously improved. The invention also provides a fermentation method for producing the epothilone B by the fermentation culture of the sorangium cellulosum by using the sorangium cellulosum fermentation medium.

The invention relates to a method for the preparation of atorvastatin adsorbates and solvates thereof, wherein one starts from a solution comprising the pharmaceutical active pharmaceutical ingredient substantially dissolved therein, one suspenses an adsorber material therein selected from the group of the celluloses, cellulose derivatives, polyols, sugars, sugar derivatives, maltodextrins, cyclodextrins, starches, polydextroses or mixtures thereof, and one removes the solvent by drying. Also, the invention relates to atorvastatin adsorbates obtainable according to this method as well as pharmaceutical formulations comprising them.

The invention belongs to the technical field of food processing, and particularly relates to a preparation method of beef black bean sauce. The preparation method comprises the following steps: cleaning soybeans, spraying saturated water vapor to the surfaces of the soybeans at the temperature of 60 to 70 DEG C for treating, uniformly mixing the soybeans with aspergillus niger for fermentation, after fermentation is ended, mixing a mixture with sorangium cellulosum for fermentation again, cleaning beef, carrying out pelletizing, mixing the beef with monascus for fermentation, after the fermentation is ended, inoculating morel strains for continuous fermentation, carrying out infrared drying step by step on the fermented beef, carrying out mixed quick freezing on the fermented beef and the soybeans according to a mass ratio of 1 to 2, then heating and frying, and finally adding beta-lactoglobulin and hydrochloric stachydrine. The finished beef black bean sauce is bronzing; beef is soft and easy to chew, and the black beans are flexible and chewy; the acid value (KOH) is less than 4.3 mg/g; the beef black bean sauce can be stored for 10 months at normal temperature.

The invention discloses a method for improving sorangium cellulosum's epothilone B yield by genetic engineering. The method includes: introducing a Vitreoscilla hemoglobin gene vgb into an expression vector PBEP43 to obtain a recombinant plasmid PBEP43-vgb, and then subjecting the recombinant plasmid PBEP43-vgb to electrotransformation into sorangium cellulosum So ce M4, thus obtaining a sorangium cellulosum So ce M4 recombinant strain, and realizing improving the sorangium cellulosum So ce M4's epothilone B yield. According to the invention, the electrotransformation method is employed to transform the gene of sorangium cellulosum So ce M4, thereby greatly improving the yield of epothilone B. The method provided by the invention has broad application prospects, and research in the aspect is not reported to date.

It is an object of the present invention to provide a method for producing ultrafine fibrous cellulose, which is capable of efficiently obtaining ultrafine fibrous cellulose having phosphoric acid groups with a high yield. The present invention relates to a method for producing fibrous cellulose having a fiber width of 1000 nm or less, comprising: a (A) of introducing phosphoric acid groups into cellulose fibers to form crosslinked structures via the phosphoric acid groups, thereby obtaining crosslinked phosphorylated cellulose fibers, a (B) of breaking some or all of the crosslinked structures to obtain crosslink-broken phosphorylated cellulose fibers, and a (C) of performing a mechanical treatment on the crosslink-broken phosphorylated cellulose fibers to obtain fibrous cellulose having a fiber width of 1000 nm or less, wherein, in the (A), crosslinked structures in an amount of 0.05 mmol/g or more and 2.0 mmol/g or less are formed, and the (B) is a step of performing the hydrolysis of the crosslinked structures in an aqueous solvent with pH 3 or more.

The present invention relates to nucleic acid sequences and proteins derivable therefrom that have been identified in Sorangium cellulosum, which proteins are catalytically active or participate in the biosynthetic pathway of disorazoles. The invention provides novel sequences which are necessary components of the disorazole biosynthetic pathway in addition to genes dszA-D.

The invention discloses a method for treating uranium enrichment biomass through combination of phanerodontia chrysosporium and modified activated carbon and application thereof. The purpose is to solve the problem that currently, corresponding uranium enrichment biomass pre-treatment methods are scarce so that the requirements of uranium enrichment biomass treatment cannot be met. According to the method, thiobacillus ferrooxidans are used for degrading the uranium enrichment biomass lolium perenne, the phanerodontia chrysosporium is used for secondary degradation of residues, and finally, the activated carbon is used for conducting adsorption treatment on uranium generated by degradation to achieve enrichment treatment of the uranium in the uranium enrichment biomass. Furthermore, according to the method, through orthogonal experiment optimization, the material dosages in the technology are greatly reduced, and the cost and the energy consumption in the technology are reduced, whichis more beneficial to application and development of the industry of the method; the phenomenon that the method has a better degradation effect on lignin, hemicellulose and cellulose in the uranium enrichment biomass, higher adsorption efficiency on the uranium, and important significance to reduction and harmlessness treatment conducted on the uranium enrichment biomass is verified.

The invention discloses a novel TALEN carrier suitable for sorangium cellulosum and a construction method of the novel TALEN carrier. The construction method comprises the following steps: firstly, replacing a promoter suitable for eucaryon with a promoter P43 suitable for escherichia coli and bacillus subtitles; and transferring TALEN elements of a left arm and a right arm of the successfully constructed target gene and the target gen into the sorangium cellulosum, and validating whether the recombinant protein is expressed with Western blot, so as to validate that the constructed carrier can perform the function of knocking out the gene in the sorangium cellulosum. The application range of a TALEN technique is further expanded; and the development of a biotechnology is promoted.

A sorangia cellulosum and a method to prepare Phoxalone from the fermentation of the Sorangia cellulosum and the applications thereof pertain to the technical field of bio-engineering and natural medicine. The invention relates to a strain of high active anti-cancer substance - Sorangium cellulosum MWXAB-125 (So ce MWXAB-125), so as to produce Phoxalone and derivatives and analogs of Phoxalone from the fermentation of Sorangium cellulosum; the invention also relates to the preparation methods thereof, the identifications of substance structures thereof and the extrinsic anti-tumor activities as cell inhibitors.

The present invention refers to gene expression cassettes encoding enzymes involved in the metabolic pathway for the biosynthesis of hemicelluloses, cellulose and/or uronic acids, and to a method for genetic transformation of plant cells through the introduction of one or more gene expression cassettes from the present invention, and the overexpression and repression of these genes in plants.

More particularly, the present invention refers to a method for introducing expression cassettes in plants. Additionally, the present invention refers to genetically modified plants.

The present invention also refers to the attainment of the genetically modified plant, its derived plants and seeds, as well as the wood, paper and cellulose from this plant.

The invention discloses a method for starting livestock and poultry manure compost secondary fermentation by utilizing oyster mushrooms. The method includes the steps of: inoculating pleurotus ostreatus mycelium into straw debris for pre-culture, and then spreading the pre-cultured straw debris on the primary fermentation product of the livestock and poultry manure to form a fermentation substrate; fermenting the fermentation substrate for the second time; or paving the straw fragments on the primary fermentation product of the livestock and poultry manure, and inoculating oyster mushroom mycelia on the straw fragments to obtain a fermentation substrate; and fermenting the fermentation substrate for the second time. The method has the beneficial effects: (1) straw cell walls are damaged and hemicellulose-cellulose is dissolved out after corn straw chippings are subjected to oyster mushroom pretreatment, so that a basis is provided for functional bacteria to convert hemicellulose-cellulose into fulvic acid after the functional bacteria are started; (2) the oyster mushrooms are pre-cultured, and the problem that the oyster mushrooms are difficult to grow in a high-nitrogen substrateis solved through an interlayer covering and adding means, so that the oyster mushrooms are smoothly colonized in a pile body;