393 results about "Secondary metabolism" patented technology

The invention provides methods employing iterative cycles of recombination and selection / screening for evolution of whole cells and organisms toward acquisition of desired properties Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and / or secretion of proteins and secondary metabolites.

A process of increasing the cellular production of secondary metabolites, such as antibiotics, is provided. The process is particularly useful for increasing antibiotic production by bacterial cells, especially erythromycin. The process includes the step of increasing the activity of methylmalonyl-CoA mutase.

Methods and kits for isolating RNA are provided that enable rapid RNA preparation from biological sources. In one aspect, RNA is isolated from difficult plant tissues and cells that contain high levels of secondary metabolites, without employing organic extraction or salt precipitation procedures. This method employs novel lysing and binding conditions to allow preparation of RNA free from secondary metabolites.

The invention provides a preparation method of an active seaweed extract. The method comprises the steps of: seaweed selecting, impurity removing, weak acid washing, mincing and homogenizing, curing and sterilization under high temperature and high pressure as well as polysaccharide component degradation, pH adjustment, probiotic fermentation, and adding of alginate oligosaccharide for blending. The obtained liquid product is an active seaweed extracting solution, or it can be prepared into active seaweed extract powder through drying, and the extracting solution and the extract powder are collectively called an active seaweed extract. The active seaweed extract prepared in the invention reserves algae iodate, mannitol, inorganic salts, trace elements and other components, and the proteinand polysaccharide components in seaweeds are degraded and easy to absorb. Added with probiotics, secondary metabolites, and low molecular weight seaweed polysaccharide, the active seaweed extract ofthe invention has better biological activity, and can remove harmful heavy metals so as to improve the security of the seaweed extract.

In a method for altering a nutritional profile of a plant, a nucleic acid sequence is selected for its ability to encode a protein capable of modifying the utilization of a substrate in the sugar alcohol secondary metabolic pathway of a plant. The protein is non-native to the sugar alcohol secondary metabolic pathway. Plant cells are transformed with an expression cassette containing the nucleic acid sequence. Genetically altered plants are recovered from the plant cells, the plants having an altered nutritional profile relative to wild-type plants. Genetically modified plants having improved nutritional profiles, and obtainable by the above method, are also provided.

The invention discloses cordyceps cicadae wine and a preparation method thereof. The cordyceps cicadae wine is obtained by dipping coremium of paecilomyces cicadae and / or mycoplasma, as raw materials, in base wine, or obtained by mixing an extract of the coremium of paecilomyces cicadae and / or an extract of mycoplasma, as raw materials, with drinking wine. In the cordyceps cicadae wine, the raw materials are fully utilized, activated secondary metabolism components in the mycoplasma are effectively kept, the integrity of active components in the cordyceps cicadae wine is ensured, the product quality is stable and the production cost is low.

Methods for improving the production of a secondary metabolite by a fungus by increasing the yield or productivity of the secondary metabolite produced by the fungus are described. The methods include increasing the expression of LYS14, for example, by transforming a cell with a nucleic acid molecule encoding LYS14.

Disclosed is a method and a product of a chicory root product for reducing taint in animals, said method comprising feeding to an animal a chicory root product during at least one day prior to slaughtering the animal. By feeding animals with the chicory root product this improves the quality of meat, prevents or reduces female and male animal taint, primarily boar taint caused by skatole and / or androstenone. The invention also relates to methods for improving the health status of animals e.g. by reducing infections by pathogens in the gastrointestinal tract and to methods for reducing animal caused odors in general. The chicory root product comprises inulin / fructan (fructo-oligosaccharides), other low molecular weight sugars and secondary metabolites.

The invention relates to azaphilones compounds in marine fungus HK1-6 and an application of the azaphilones compounds as an MRSA-resistant drug, in particular to secondary metabolites 1-4 produced bymarine fungus Penicillium sp.HK1-6 or pharmaceutically acceptable salt. The compounds 1-4 show very high antibacterial activity for MRSA (such as S. aureus ATCC4330 and S. aureus ATCC33591) and VER (such as E. faecalis ATCC51299), MIC is smaller than or equal to 12.5 mu g / mL, the MIC of a compound 2 for E. faecalis ATCC51299 is one times higher than that of positive drug vancomycin, and the compounds have good prospects to be developed into drugs resisting drug-resistant bacteria, lead compounds of drugs and candidate drugs.

A fumigant pesticide composition made up of a base formula and enhancement formula, which include botanical essential oil-based active ingredients and inert materials identified under the United States Environmental Protection Agency's approved list of minimum risk pesticides. The enhancement formula is effective to inhibit or enhance the release of the secondary metabolites of the essential oil-based active ingredients. An apparatus and method for the controlled release of the fumigant pesticide composition is also disclosed.

The invention discloses a biologic emulsifier for livestock feed. The biologic emulsifier is characterized by being prepared by two or more than two of sophorolipid, rhamnolipid and trehalose. The sophorolipid, the rhamnolipid and the trehalose and the like in the emulsifier are all secondary metabolites generated by microbial fermentation, can be naturally metabolized and degraded, do not cause residues in animal bodies, and do not influence quality of animal products. The biologic emulsifier can remarkably improve livestock fat digestion utilizing rate, improves feed reward, reduces livestock blood cholesterol and triglyceride, remarkably improves livestock production performances, improves carcass quality, improves body immune function, reduces morbidity and mortality, and remarkably improves economic returns.

The invention discloses a genetic engineering strain for producing phenazine-1-carboxylic acid, which is produced by taking pseudomonas chlororaphis as a culture, as well as application of the genetic engineering strain; the genetic engineering strain is obtained by knocking out a phzH gene in pseudomonas chlororaphis genome. The genetic engineering strain for producing phenazine-1-carboxylic acid is finally prepared by deleting the phzH gene in the genome of the strain by virtue of insertion mutation or marker-less deletion from pseudomonas chlororaphis HT66 CCTCC NO: M2013467 which can naturally secrete phenazine-1-formamide as well as derivatives of the pseudomonas chlororaphis so as to convert a secondary metabolite from the phenazine-1-formamide into the phenazine-1-carboxylic acid. The invention also discloses a preparation method and a detection method of the fungicide.

The present invention provides a method of making a genetically transformed plant which has an altered content of at least one product of a secondary metabolic pathway. The method consists of introducing into a plant cell capable of being transformed and regenerated to a whole plant a DNA expression cassette. The expression cassette includes DNA sequences required for transformation and selection in plant cells. It also includes a DNA sequence that, under the control of a promoter active in plant cells, encodes a protein capable of modifying the utilization of a substrate in the secondary metabolic pathway. The substrate is not a primary metabolite of the group selected from glucose, amino acids, common fatty acids and nucleotides. A plant or plant tissues including seeds can then be recovered having an altered content of at least one product of the secondary metabolic pathway. The invention also provides for feed products derived from the plants and seeds obtained according to the method.

The invention discloses a method for increasing pulullan yield by sub-sectional adding of growth factors in a fermentation process, and belongs to the technical field of biological fermentation engineering. The method comprises the following steps: producing pulullan from aureobasidium pullulans CGMCC NO.7055 in a fermentation manner; adding 0.003-0.005% of vitamin B1 when bacteria growth is at the beginning of the logarithmic phase; and adding 0.002-0.004% of uridine monophosphate when the bacteria growth is at the early stage of a stable stage, so as to induce and facilitate accumulation of secondary metabolite, namely the pulullan. The growth factors are specifically added according to the characteristics of sugar production in different growth periods and fermentation processes, so that the method is convenient and fast to operate and has an obvious effect; the fermentation period is greatly shortened, the transformation rate of a substrate is increased, and the cost of the pulullan is reduced.

The invention provides a soil improvement formula. The soil improvement formula comprises a soil conditioner, a microbial agent and biostimulant, the soil conditioner is prepared from kitchen organicwaste, and the microbial agent is prepared from 5 wt%-10 wt% of actinomycetes, 10 wt%-20 wt% of nitrogen-fixing bacteria, 5 wt%-10 wt% of paecilomyces lilacinus, 10 wt%-25 wt% of trichoderma viride, 5wt%-20 wt% of lactic acid bacteria and 20 wt%-35 wt% of bacillus subtilis. The biostimulant comprises seaweed extract and / or crustacean extract, wherein the mass ratio of the soil conditioner to themicrobial agent to the biostimulant is (300-800): (150-200): (60-120). According to the soil improvement formula, the secondary metabolism level of apples can be increased, and the external light color and flavor of fruits are improved. The invention further provides an application of the soil improvement formula in apple planting.

The invention discloses a biologic emulsifier for aquatic animal feed. The biologic emulsifier is characterized by being prepared by two or more than two of sophorolipid, rhamnolipid and trehalose. The sophorolipid, the rhamnolipid and the trehalose and the like in the emulsifier are all secondary metabolites generated by microbial fermentation, can be naturally metabolized and degraded, do not cause residues in aquatic products, and do not influence water quality. The biologic emulsifier can remarkably improve feed fat digestion utilizing rate of fishes, shrimps and crabs and the like, reduces bait coefficient, remarkably improves production performances of aquatic animals, improves body immune function, reduces morbidity and mortality of the animals, and remarkably improves economic returns.

A method for increasing the content of taxol in enqlish yew features that the solution of coronary bacteruim extract onto the stem and leaves of young enqlish yew for regulating its secondary metabolism system and increasing the content of taxol.

The invention provides methods employing iterative cycles of recombination and selection / screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and / or secretion of proteins and secondary metabolites.

The invention discloses glycosyl transferase participating in neoandrographolide biosynthesis as well as coding genes and application thereof. The protein provided by the invention is derived from herba andrographitis, and is (a1) or (a5) as follows: (a1) a protein composed of an amino acid sequence shown as a sequence 1 in a sequence table; and (a5) a protein composed of an amino acid sequence shown as a sequence 3 in the sequence table. The invention further discloses application of the protein serving as glycosyl transferase. The glycosyl transferase participates in plant growth and development and secondary metabolism processes. The discovery of the glycosyl transferase and coding genes thereof has important significance. The invention provides a basis for further describing biosynthesis of glycoside compounds in the herba andrographitis, contributes to analyzing a biosynthetic pathway of glycoside active ingredients in the herba andrographitis, and provides novel material basis and gene resources for new drug research and development.

Disclosed herein are biocontrol compositions against plant fungal pathogens and methods of use thereof for the prevention or reduction of crop loss or food spoilage. The biocontrol composition can comprise at least one microbe with anti-fungal activity or a secondary metabolite of the at least one microbe. The methods can comprise application of the biocontrol composition to a plant, a seed, or aproduce thereof or to a packaging material used to transport or store the produce.

The invention discloses a prawn ecological water quality modifier which is characterized in that: prawn ecological water quality modifier is prepared by taking the activated shuck powder as a carrier and the high-activity secondary metabolism product medicine extracted from the fermentation liquid of Chinese herbal medicine as active ingredient. The prawn ecological water quality modifier disclosed by the invention has the advantages of simple preparation method, low cost and strong disease resistance, maintains stability of the ecological environment of prawn pond, and avoids pollution.

The invention discloses a method for hydroponic breeding of huperzine serrate and induced improvement to huperzine a of the huperzine serrate. According to the method, wild naturalized huperzine-serrate branches with stem tips are used as cuttings, and rapid hydroponic breeding of the huperzine serrate is achieved in a nutrient solution containing hormones and precursor substances with a certain ratio; compared with conventional soil cutting, the method realizes that rooting time is short, the breeding coefficient is large, and the rooting percentage is as high as 98%. The precursor substances enable the huperzine a content of the hydroponic huperzine serrate to be increased by 70% compared with wild plants; the leaf surfaces or / and the roots of the plants are sprinkled with elicitors in a certain concentration, so that a secondary metabolism system of the huperzine serrate is regulated, the huperzine a content of the hydroponic huperzine serrate is increased 1.3-1.6 times, the yield of the huperzine a is increased, and the situation of shortage of huperzine-serrate resources is alleviated to a certain degree. Besides, the method achieves low production cost and a short cultivation period, is not restricted by the natural environment and climate, and has broad application prospects and high popularization value.

The invention provides a technology for extracting genomic deoxyribonucleic acid (DNA) from a recalcitrant plant, and belongs to the technical field of biology. The technology is characterized in that secondary metabolism substances such as polysaccharide and polyphenol can be successfully separated from the recalcitrant plant by utilizing eluent, and the problem of ropiness of a centrifugal supernatant is solved; phenol, chloroform and isoamyl alcohol are utilized to conduct the extraction, then the chloroform and the isoamyl alcohol are utilized to conduct the extraction, and protein, the phenol, and the like can be completely extracted; and polyphenol substances can be prevented from being oxidized into quinines and the browning is avoided, so that high-quality genomic DNA of the of the recalcitrant plant is successfully extracted. The technology is mainly applied to the extraction of genomic DNA of plant materials of which cells contain plenty of secondary metabolite such as the polysaccharide, the polyphenol, tanned matters, cinnamaldehyde, cinnamic acid, and the like.

The invention belongs to the field of molecular biology and relates to a detection technology for separation and clone, site-specific mutagenesis, zymoprotein prokaryotic expression, zymoprotein separation and purification and enzyme functions of a branch-point key enzyme gene participating in synthesis of an isoprenoid matter in a wheat mevalonic acid metabolic pathway. The method can provide important technical storage for further performing the gene improvement and modification, constructing an eucaryon gene expression carrier of a farnesyl diphosphate synthase gene, and converting corresponding crops; secondly can be used for obtaining plants with important commercial values in virtue of secondary metabolism, and discussing a relationship of the overexpression of the farnesyl diphosphate synthase gene in a receiver plant and important agronomic traits such as secondary metabolites, crop grain sizes and crop grain weights; and improving the yield of the crops, analyzing structures of introns, exons and promoters, studying functions of the promoters, developing related molecular markers.

This invention is a method of minimizing the variation of cell growth and production through homogeneous cell line development. To be more specific, it is the method of isolating and proliferating single cell clone from the procambium or cambium to promote the stability of the plant-derived biologically active substances production by solving the problems of decrease in cell growth and the productivity during the long term culture. Key words: Procambium or cambium, single cell clone, cell aggregation, dedifferentiation, cryoperservation, variability.

The invention belongs to the field of preparation and utilization of secondary metabolism active components of forestry processing residues, and concretely relates to a technology for preparing a caffeoyl group-containing tannin derivative 1,3-di-O-galloyl-6-O-caffeoyl-beta-D-glucopyranose from Juglandaceae Juglans Juglans sigillata tree leaves, and an application of the derivative in the preparation of anti-oxidation products. The technology has the advantages of standardized extracting and separating preparation process, high yield, low cost and simple operation.

The invention discloses a preparation method for a dichloro substituted II-type halogenated polyketone compound zunyimycin A, wherein the compound is derived from a secondary metabolite of a streptomyces sp.FJS31-2 strain (with the strain preservation number of CGMCC4.7321), and finally, a structure is identified and determined after strain activation, expansion culture and isolation. The compoundcan be used in treatment of diseases infected by gram-positive bacteria such as staphylococcus aureus (MRSA) and drug-resistant bacteria thereof, staphylococcus epidermidis, bacillus subtilis and thelike, is used in treatment of diseases infected by gram-negative bacteria such as super-spectrum beta-lactamase escherichia coli (ESBL), proteusbacillus vulgaris, bacterium burgeri and the like, andis used for treatment of diseases infected by fungi, such as candida albicans.