The invention relates to a method for culturing a siraitia grosvenorii callus cell suspension system. The method comprises the following steps of: 1) inoculating and inducing siraitia grosvenorii stem calluses, performing subculture on MS liquid medium added with 1-naphthaleneacetic acid (NAA) hormone, sucrose and agar, and selecting the calluses; 2) transferring the calluses obtained in the step 1) to a sterile triangular flask, putting the triangular flask into a shaking incubator, performing suspension culture for 24 hours under the dark condition of 25 DEG C, transferring the culture medium of the upper layer, single cells and small cell masses to a triangular flask, and continuously performing shaking culture, performing transfer again after 3 days by adopting the same method, filtering the suspended cells through a screen with meshes of 40 microns after 7 days, inoculating the suspended cells to fresh culture medium for culturing, transferring the cultured cells to a big triangular flask after a certain volume is reached, and continuously adding the culture medium and performing shaking culture by using the same method; and 3) culturing for 21 days, harvesting, flushing for 3 times, performing vacuum filtration, weighing and drying. The method has the characteristics of high propagation speed and large culture scale, and provides a large amount of uniform plant cell cultures.