Method for establishing fraxinus mandshurica suspension culture system

A culture method and system technology, which are applied in the field of plant cell engineering to achieve the effects of industrialized production, low production cost and fast cell reproduction.

Inactive Publication Date: 2013-01-23
NORTHEAST FORESTRY UNIVERSITY +2
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  • Abstract
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However, the use of callus regeneration and s

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  • Method for establishing fraxinus mandshurica suspension culture system
  • Method for establishing fraxinus mandshurica suspension culture system

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Embodiment Construction

[0042] The object of the present invention is to provide a method for establishing a suspension culture system of Fraxinus Fraxinus Fraxinus Fraxinus Fraxinus, which is easy to implement, convenient to operate, fast in proliferation, and can accelerate the rapid propagation of Fraxinus Fraxinus Fraxinus.

[0043] In order to achieve the above object, the present invention adopts the following technical measures:

[0044] 1. Take Fraxinus mandshurica stems as explants, soak them in 75% alcohol for 10s or 20s or 25s or 28s or 30s, and use 0.1% Ca(ClO) 2 Treat for 2 minutes or 3 minutes or 5 minutes, rinse with sterile water 3 times or 4 times or 5 times or 6 times;

[0045] 2. Cut the sterilized stem section into small sections of about 0.5-2cm with a sterile knife in a petri dish, and inoculate it in a solid medium. The effective components of the medium are: WPM medium and additional components. The additional components are:

[0046] TDZ 0.1-1.0mg / L+NAA 0.5-2.0mg / L+agar 50...

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Abstract

The invention provides a method for establishing a fraxinus mandshurica suspension culture system. The method comprises the following steps of: 1) using a fraxinus mandshurica stem section as an explant, inducing callus, and obtaining the callus on a WPM solid culture medium added with hormone TDZ and BA, cane sugar and agar; 2) inoculating the callus obtained in the step 1) into the WPM liquid culture medium added with hormone BA and 2, 4-D and cane sugar, and oscillating and culturing; and 3) carrying out subculture for 10-15days, and continuously carrying out subculture for 3-5 times to obtain a suspension cell line which uniformly grows and is rapidly propagated. The method has the characteristics that the propagation speed is high, the culture scale is large and a great amount of uniform plant cell cultures are provided.

Description

technical field [0001] A method for establishing a suspension culture system of Mandshurica mandshurica by using basic medium and adding different hormone components, the invention belongs to the technical field of plant cell engineering. Background technique [0002] Mandshurica mandshurica (Fraxinusm andshu rica.) belongs to the genus Ash in the Oleaceae family. It is a tall deciduous tree. It is one of the "three hard broads" that are precious in Northeast my country. The wood material is fine and the texture is beautiful, and it is widely used in various fields. Studies abroad have found that its bark contains coumarin components, including Esculin, Esculetin, Fraxin, Isofraxidin, Mandshurin, Fraxinol). Coumarin compounds can not only directly inhibit cancer cells, but also produce anti-cancer effects by enhancing the body's immunity. But at present, the coumarin compounds mainly come from the bark and leaves of the ash tree of Oleaceae, which greatly causes the waste ...

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Application Information

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IPC IPC(8): C12N5/02C12N5/04C12R1/91
Inventor 齐凤慧詹亚光曾凡锁
Owner NORTHEAST FORESTRY UNIVERSITY
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