1184results about How to "Reproduce fast" patented technology

The invention relates to a plant seedling raising technique, in particular to a camellia oleifera container seedling raising technique which aims to solve the problems of low seedling survival rate and low seedling raising efficiency of a camellia oleifera seedling raising technique in the prior art. The camellia oleifera container seedling raising technique includes the steps of (1) collecting seeds, and placing the collected seeds in a refrigeratory for cold storage and reserve at the temperature of 0-5 DEG C; (2) pre-treating the seeds, and soaking the seeds prior to sand storage for accelerating germination; (3) sowing, sowing the camellia oleifera seeds subjected to germination accelerating in a container containing seedling raising substrates in a spot manner, and then placing the container on a seedbed; (4) grating rootstocks of seedlings; (5) performing grafted seedling management including water management, rootstock removal and weeding management, light and temperature management, nutrient management and the like; (6) performing biennial seedling production management; and (7) performing outplanting management. The technique has the advantages of high seedling survival rate, high breeding efficiency and good reliability; and seedling survival rate of the seedling raising technique is 10-20% higher than that of inarching seedling raising and leaf cutting seedling raising techniques.

The invention discloses a method to prepare leaven of fertilizer and application, which is characterized by the following: allocating raw material with mass ratio at 10-80 wt industrial residue of ferment, 5-30 wt straw, 5-30 wt cake meal, 10-30 wt bran or rice bran, 1.5-2 wt aquacare, 0.5-1 wt NaCl and or amylaceum, and 1-30 wt brown ball Azotobacter (Azotobacter chroococcum), 1-30 wt bacillus megaterium (Bacillus megaterium), 1-30 wt bacillus licheniformis (Bacillus licheniformis), 1-30 wt plant lactobacillus (Lactobacillus plantarum) and 1-30 wt 1-5 strain of saccharomycopsis fibuligera (Saccharomycopsis fibuligera); producing the product.

The invention relates to a compound microbial organic fertilizer and a preparation method thereof. The compound microbial organic fertilizer is prepared from the following ingredients in parts by weight: 1-4 parts of trichoderma viride JUWEI002 fermentation liquor, 1-4 parts of bacillus subtilis JUWEI001 fermentation liquor, 20-30 parts of turfy soil, 33-38 parts of tobacco residue, 33-38 parts of silkworm faeces, 6-7 parts of calcium powder and 1-2 parts of potassium fulvate. The compound microbial organic fertilizer disclosed by the invention has the advantages that the quality of crops can be remarkably improved, the yield of the crops is increased, and the soil structure is improved.

The invention discloses a cutting propagation method for lithocarpus polystachyus rehd, which includes: preparing a cutting land, preparing a seed bed and preparing cuttings; cutting shoots, disinfecting, obtaining cuttings, coating a base of each cutting with rootage pulp, performing cottage and performing field management; dredging for drainage, intermittently venting the cuttings, timely watering to preserve moisture for the cuttings, and timely disinfecting and exercising cutting seedlings ; removing plastic of an arch transparent plastic greenhouse on the seed bed, timely spraying organic fertilizer to the cutting seedlings and further exercising the seedlings; and removing a sunshade set to obtain the seedlings on about the ninetieth day to the hundredth day after cuttage. The cutting propagation method for lithocarpus polystachyus rehd has the advantages that the cuttings start growing new roots and new buds on about the thirtieth day after insertion into the seed bed, the new roots and the new buds of the cutting seedlings are in the peak of growth, abundant and long new roots are grown, survival rate of the cutting seedlings reaches above 80%, transplanting survival rate is also above 80%, and propagating of lithocarpus polystachyus rehd is greatly quickened.

The present invention belongs to the field of plant cultivating technology. The cuttage process of photinia frasery includes the following steps: forming seedling bed, building arced shed and spreading medium for cuttage; picking from the mother body semi-lignified young follage or the same year branch for cutting shoot and cutting into cuttage branch of 3-4 cm length with one bud; treating the cuttage branch with rooting agent; planting in the medium of seedling bed in the depth of 2-4 cm and proper density; watering, spraying germicide to branch and leaf, covering with plastic film and shading net and management after planting. The said process has survival rate as high as 90-95 % and fast propagation speed in obtain excellent photinia frasery.

The invention relates to a method for cultivating sweetened mango reversed to season, wherein it comprises fertilizing, cutting, accelerating flowering, inducing flower bud, resisting pest, fertilizing, sheering crown. The invention has low consumption of agricultural chemicals, and high yield.

The invention discloses an unpolluted, green and high-efficiency composite biological soil modifier which comprises the following components: trichoderma pseudokoningii fermentation liquor, bacillus subtilis fermentation liquor, water-soluble chitin, potassium humate and decomposed organic matter. The trichoderma pseudokoningii and the bacillus subtilis are high-efficiency bacterial strains which are obtained through screening and mutating, the trichoderma pseudokoningii fermentation liquor and the bacillus subtilis fermentation liquor are obtained by a liquor submerged fermentation technology, and the unpolluted, green and high-efficiency composite biological soil modifier can be prepared in a combination way. The soil modifier has the following functions that the physicochemical property of the soil can be improved, the hardening of the soil can be broken up, the volume weight of the soil can be reduced, the air permeability of the soil can be improved, the microbiological activity of the soil can be promoted, the growth of the plant root system can be promoted, the disease resistance of the crop can be enhanced, the yield of the crop can be improved, the quality of the crop can be improved, the original ecology of the crop can be recovered and the like, and the soil modifier is the novel soil modifier which is high-efficiency, environment-friendly and convenient to use.

An object of this invention is to provide a method which, when connection to any external apparatus is established, allows a coded video file to be supplied to the connected external apparatus in a format appropriate for the file format of the external apparatus, and with high video quality, and further achieves immediate responsiveness from connection to reproduction and display. The video-coding apparatus 1 is provided that includes: an apparatus registration block 13 which, when an external apparatus 2 is connected, acquires apparatus information 103 from the connected external apparatus 2 and automatically registers the external apparatus; a coding block 10 that encodes programs in a file format appropriate for at least one external apparatus 2 registered for the same program, and generates a coded video file 101; a storage block 11 which stores the coded video file 101 that has been generated; and an output file selector block 12 which, when the external apparatus 2 is connected, selects a coded-video file 102 of a file format appropriate for the particular external apparatus 2, from the coded video files of multiple formats that contain the same program stored within the storage block 11, and outputs the selected video file 102 to the external apparatus 2.

The invention relates to a complex microorganism fungicide for treating animal manure and a preparation method and an application thereof. The adopted technical scheme is that the complex microorganism fungicide consists of saccharomyces cerevisiae, powdery pichia, bacillus subtilis, bacillus cereus, bacillus megaterium and lactobacillus plantarum. Microbial organic fertilizer can be prepared by treating the animal manure with the complex microorganism fungicide. The complex microorganism fungicide for treating the animal manure prepared by the invention has the advantages of easy obtainment of raw materials and simple manufacturing method. Preparing the microbial organic fertilizer by mixing the complex microorganism fungicide for treating the animal manure with the animal manure, organic materials and cornmeal not only can treat the animal manure, but also effectively utilizes rural wastes; and the prepared microbial organic fertilizer can not pollute crops and cultivated land because no chemical substance exists in the prepared microbial organic fertilizer.

The invention provides a special compound microbial fertilizer for paddy rice. The compound microbial fertilizer comprises the following components: an organic fertilizer component, an inorganic compound fertilizer component, bentonite and a compound microbial component; the compound microbial fertilizer contains 10-15% of nitrogen, 4-8% of phosphorus, 6-10% of potassium, 25% of organic matters and 2-18 hundred million c f u / g of compound microorganisms. According to the invention, comprehensive nutrition required by the growth of the paddy rice can be met; the quality of the paddy rice is improved and the increase in both yield and income is realized; moreover, the soil fertility of the rice field can be improved, the soil environment of the rice field is improved, and the occurrence of rice diseases is effectively prevented.

The invention provides a microbial fertilizer for improving saline alkali soil. The microbial fertilizer comprises the following raw materials in parts by weight: 45-55 parts of turf, 25-35 parts of Angel yeast residues, 5-15 parts of monoammonium phosphate, 5-15 parts of ammonium sulfate and 1-3 parts of a complex microbial inoculant. According to the microbial fertilizer provided by the invention, the microbial flora is in mutual promotion with organic matters and inorganic matters, the components supplement one another, the effective living bacteria count is not less than 50million per gram, the content of the organic matters is not less than 55 percent, the total nutrient is not less than 11 percent, the content of ammonium nitrogen is not less than 4 percent, the content of P2O5 is not less than 4 percent, the content of K2O is not less than 3 percent, and the pH value is 5.5-6.0. Under the co-actions of the components, the effects of rapidly reducing the soil salinity and alkalinity and increasing the content of organic matters, available phosphorus and ammonium nitrogen are achieved, and the conditions that the granular structure of the soil is poor, the soil permeability is reduced and the activity of the microbial flora is inhibited are improved.

The invention discloses a burkholderia pyrrocinia JK-SH007 which is classified and named as burkholderia pyrrocinia and preserved in China center for type culture collection (CCTCC) with the preservation number of M 209028 and the preservation date of February 18th, 2009. The invention also discloses the application of the burkholderia pyrrocinia in controlling dothiorella gregaria and promoting poplar to grow. The invention further provides a bactericide for controlling dothiorella gregaria, which is obtained by inoculating the JK-SH007 in a culture medium to be fermented and cultured. The JK-SH007 is plant endophyte which has strong inhibiting effect for botryosphaeria dothidea of the poplar and antagonism for various pathogenic fungis, and has the advantages of simple culture condition, easy storage and commercial process as well as good developing and applying prospect.

The invention discloses a submerged plant propagating and culturing device which comprises a bearing support, a buoyancy support and a substrate layer, wherein the buoyancy support is ring-shaped and is arranged on the upper end surface of the bearing support, and the substrate layer is laid on the upper end surface of the bearing support and at least comprises a non-woven fabric layer used for fixing the root system of a submerged plant and a nylon mesh layer arranged below the non-woven fabric layer. The invention also discloses a method for propagating and culturing submerged plants. Compared with the prior art, the invention has the following advantages that a special device is utilized for changing the growing environment in different water depth conditions, and an appropriate growing condition is provided to speed up growth of the submerged plants so as to improve the propagation speed. The submerged plant propagating and culturing device and the method do not have quite high requirements on water depth, the thickness of soft bottom sediment and water flow stability; and the overall cost is low, the operation is simple and convenient, and the submerged plant propagating and culturing device and the propagating and culturing method are applicable to mass propagations.

The invention provides a method for rapidly culturing shoot tips in vitro, which is characterized in that in the first step, selecting stolon of the strawberries sprouted at the same year, washing to be clean and disinfecting; in the second step, cutting 0.1-0.5 mm shoot tips of the disinfected and cleaned strawberries stolon under the anatomical lens, inoculating to a culture medium for germination culture, cutting the new sprouts after the 0.5-1.5 cm new sprouts are grown on the shoot tips, inoculating to an enrichment medium for inducing clustered shoots; in the third step, cutting the cluster buds to simple buds, inoculating to a rooting medium for rooting culture; transferring test tube plantlets to a substrate formed by mixing perlite and peat, watering and covering a plastic film for insulation and moisture retention, revealing the plastic film for gradually hardening, transferring the seedling to a plastic potted tray filled with pure peat, watering and covering the plastic film for insulation and moisture retention, and directly planting the seedling to a field. The invention has the advantages of high reproductive rate, rapid reproduction speed and high survival rate.

The invention discloses a method for semi-lignified twig cutting breeding of Aquilaria sinensis, which comprises: collecting wide high-quality Aquilaria sinensis seeds, sowing the seeds for raising seedlings and building a cutting orchard with high-quality seedlings; in the following year, trimming the seedlings in the cutting orchard at the place which is more than or equal to 15 centimeters away from the ground, and applying 6-BA on the trimmed seeding stems for promoting the germination of twigs; collecting materials when the twigs grow to a semi-lignified state by cutting the twigs to 10 to 15 centimeters with three top leaves left and each leaf having 2 / 5 leaf area; cutting the cuttingwood with a flat cut, obliquely cutting the lower end of the cuttingwood at a position as close to leaf segment as possible; cleaning the cutting wood, disinfecting, plugging the lower part of the cutting wood into prepared plant growth regulator treatment solution, inserting the cutting wood into a medium, performing post-cuttage management and obtaining Aquilaria sinensis seedlings. The method is low in cost, ensures high survival rate, high seeding quality and quick propagation, relieves seed shortage situation, keeps the excellent hereditary characters of the high-quality trees, is easy to promote and has a promising development prospect.

The invention provides a method for breeding medical ground beetles and belongs to the technical field of medical animal breeding. The method for breeding the medical ground beetles comprises the steps of control over a place of a breeding party, temperature and humidity, management of breeding fodder, breeding soil of the ground beetles, an egg reservation of the ground beetles and incubation of eggs, management of larvae of the ground beetles and medium-sized ground beetles, management of copulation, management of adults, prevention and treatment of diseases and pests, collection and processing of the ground beetles and finished product storage of the ground beetles. The method for breeding the medical ground beetles accords with the original wild life habits of the ground beetles, the growth and breeding speed of the ground beetles is greatly increased, and the ground beetles are good in medical property, strong in efficacy, good in quality and high in market selling price.

The invention relates to a method for testing the toxicity of a nanomaterial by using a transgenic zebra fish embryo model. The method comprises the following steps of: (1) adding nanomaterial test solution to be tested into zebra fish embryo culture solution which has been fertilized for 6 to 7 hours, or cultivating the mixture in an artificial climate cabinet to 72hpf or 96hpf; (2) taking the 72hpf or 96hpf embryo out, fixing the embryo with 4 volume percent paraformaldehyde for 24 to 30 hours, washing the embryo with phosphate (PBS) buffer solution for 2 to 4 times, and fixing the embryo on a glass slide for 24 to 48 hours, and observing blood vessels with a fluorescence microscope; and (3) judging whether the nanomaterial causes vascular injury or not according to an image acquired by the fluorescence microscope. The method has the advantages of readily available raw materials, simple administration manner and small dosage of the nanomaterial to be tested, simplicity, short test cycle and wide application prospect.

The invention relates to a method for preparing an ecological organic fertilizer from organic waste. The method comprises the following steps of putting the organic waste of which the moisture content is within 95% into a subcritical water reaction kettle, adding biomass which is 0-10% of the organic waste, feeding NaOH to the reaction kettle to adjust the pH to 7.5-8.5; leading steam into the reaction kettle, adjusting the temperature to 150-300 DEG C and the pressure to 0.5-5.0MPa to react for 15-60 minutes; baking or dewatering the product until the moisture content is 50-65%; feeding a microbial agent which is 0.5-1% of weight of the product to the baked or dewatered product; evenly mixing and then piling, heating the pile to 60-70 DEG C after 2-3 days to be stable, and carrying out heat preservation at 55-65 DEG C to maintain for 5-7 days, and turning when the temperature exceeds 65 DEG C and preparing the product until the temperature is not changed. By adopting the method, the limitation of the traditional aerobic fermentation is broken through; pathogene can be thoroughly killed; no bad smell or heavy metal pollution appears; the treatment cost is reduced; the treatment efficiency is improved; and the highest target of resource utilization of the organic waste is achieved.

The invention provides a production method of minitype potato seeds bred by directly using potato stem segments, relating to the minitype potato seed high power propagation technology. In the invention, potato detoxification test tube tissue culture plantlet stem segments are used as a raw material, and then are directly implanted in an MS liquid or solid induction medium without any plant growth hormone, and the minitype potato seeds capable of sprouting are induced and produced in 6-8 weeks. The tissue culture plantlet stem segments are directly bred without changing the medium, thus obtaining the potato seeds in one step. The method has the advantages of saving time, labor and cost, having simple equipment, less used plantlets, less consumed time, rapid propagation and high efficiency and the like. The breeding period is 1.5-2 months, production can be performed for 5-6 periods each year, and the breeding chamber at unit area can produce 500,000-600,000 minitype potato seeds, thus making full use of the tissue culture chamber production space, greatly improving the output of the detoxification minitype potato seeds at each unit area, and increasing the production efficiency at each unit area by 50-100 times. The method is suitable for large-scale factory production of minitype potato seeds.

The invention relates to a tissue culturing method of hemerocallis middendorfii poinsettia. The method comprises steps that: when hemerocallis poinsettia blooms, flower buds are selected, or newly grown buds are selected in early spring; sterile materials are obtained; the buds are differentiated and multiplied; adventitious bud strong seedlings are cultured; rooting culturing, acclimatization and transplantation are carried out upon the buds, such that hemerocallis poinsettia plants are obtained. Compared to prior arts, according to the invention, through the tissue culturing method, multiplication speed and nursery stock regularity can be substantially improved, original female parent properties can be maintained, survival rate can be improved, and the yield can be improved. With the method, the transplantation survival rate of hemerocallis middendorfii poinsettia reaches 95%.

The invention discloses a method using tissue-subculture sprouts of oil-tea camellia (Camellia oleifera Abel) to raise seedlings by grafting, particularly a method using the tissue-subculture sprout of high-grade oil-tea camellia as a scion and using the germinated germ of oil-tea camellia seeds as a stock to culture a novel plant in a shed sealed by plastic film at 10 to 35 DEG C with the air humidity being 100% on the basis of the method of cleft grafting or slope grafting, wherein the stock seeds are stored at 4 to 5 DEG C by humid sand in an icehouse having high grafting capacity and seed storage capacity; and the grafting process is carried out day by day, and the germination acceleration is carried out group by group, accordingly, the survival rate reaches 75% to 95%. The method of the invention has the advantages of high reproductive rate, high seedling raising capacity, simple method, easy operation and low material consumption; and meanwhile, the offspring can maintain the good hereditary property of the stock plant, thus solving the problem that a large amount of improved branches and budlings are required for the existing method for the vegetative propagation of oil-tea camellia, such as cuttage propagation and seedling-raising by germ-stock grafting, and meeting the requirements of high-grade and high-quality clonal oil-tea camellia sprouts for the development of the oil-tea camellia industry in China.

The invention relates to a cuttage propagation method of Virginia oak, which belongs to the technical field of plant cultivation. The method comprises the following steps: firstly, a sterilizing seedbed is built in a big-arch shelter, an arch shed is built on the seedbed, a hole pan provided with cuttage substrate is arranged in the seedbed, and the cuttage substrate is treated and sets aside; secondly, branches derived from close roots of the Virginia oaks grown for 2-4 years are selected to prune ears in February to March every year when sprouts do not and are about to burgeon, and the ear strips are pruned into branches; thirdly, the branches are treated by root stimulating agent, and the cuttage is done immediately, for the branches which then enter into the management after the cuttage; fourthly, after more than 70 percent of branches root and sprout, the hole pan is moved to forcibly exercise the plantlets outside the big-arch shelter, and the plants are transplanted into ground after being exercised for 30-35 days at the last third of the December or the last third of the February of the next year and enter the management after transplanting. The cuttage propagation adopting the method has high survival ratio and fast propagation speed. The invention is suitable for the climatic environments in most parts of Yangtze River Delta in China and has the survival ratio up to 80-90 percent, and the obtained nursery plantlets of the Virginia oaks have sound quality.

The invention discloses a process for treating wastewater generated in a solar silicon wafer production process and relates to wastewater treatment in the field of the electronic semiconductor industry. The process comprises the following steps of: pretreating wastewater in a preliminary sedimentation tank; carrying out Fenton and iron-carbon micro-electrolysis combined oxidization reaction on the wastewater in an iron-carbon micro-electrolysis reactor; after the reaction, feeding the wastewater into a coagulation-sedimentation integrated pool, feeding PAC (Poly Aluminium Chloride) and PAM (Poly Acryl Amide) to arouse the coagu-flocculation reaction of the wastewater, and after the reaction, performing sludge-water separation on the wastewater; causing the supernate to flow to a hydrolysis acidification pool, and then causing the wastewater to flow to a contact oxidation tank; causing the mixed liquid to flow to a secondary sedimentation tank for sludge-water separation, while causing the supernate to flow to a deflection sedimentation pool, and feeding PAC for purifying water so that the supernate is up to the stand and can be discharged after sedimentation; finally, performing concentration treatment on sludge. After non-degradable organic wastewater is treated by the process provided by the invention, the BOD5 (Biological Oxygen Demand) / CODcr (Chemical Oxygen Demand) ratio of the wastewater is obviously increased.

The invention belongs to the technical field of fast propagation of plants, comprising the following steps of: (1) building a sterilized seedling bed on a selected propagation place, laying sterilized substrate in the seedling bed with sterilized cutting substrate of turfy soil, perlite and vermiculite concrete; (2) selecting healthy 1-2 years of seedlings with no damage by disease and insect or young trees as seed tree for collecting roots, digging a semicircular groove with a distance of 0.5m from the trunk, excavating the side root with the diameter of 1-3cm as propagation material and trimming the side root into root sections of 3-5cm; (3) soaking the trimmed root sections in carbendazim solution for sterilizing, adopting IBA+NAA solution as hormone, using ABT rooting powder solution to soak the base part for inducing the root; (4) inserting the root in the substrate of the seedling bed to lead the top end to be lower than the ground slightly and to be level with the ground after the soil is coated; (5) after cottage, covering the seedling bed with mulching films, sealing the periphery of the seedling bed, building tunnels on furrows and building overhead shade nets on the tunnels; and (6) after cottage, managing, transplanting and preventing damage by disease and insect.

The invention belongs to the field of biological engineering and is aimed for solving the problem of mass scale plant diseases caused by fungus. The preparing process comprises subjecting a strong of Bacillus firmus (CGMCC No.0924) to second level fermentation culture under suitable condition, then carrying out filtering, salting out and column chromatography treatment.

The invention discloses bacillus velezensis strain HN03 and application thereof. The bacillus velezensis strain HN03 has the accession number CCTCC M 2018084. The pure bacillus velezensis strain HN03has a good inhibitory effect on banana fusarium No. 1 and No. 4 physiological strains, and various other pathogenic bacteria.

The invention discloses a cuttage rapid-propagation seedling culture method for elevated sand bed camphora wood cutting orchard tissue culture seedling twigs. The method comprises the following steps: taking semi-lignified twigs formed by camphora wood cutting orchard tissue culture seedling planted on the elevated sand bed as a cutting wood; treating the cutting wood by greenplant growth regulator-ABT6 rooting solution; inserting the treated cutting wood into a mixed medium which contains peat soil and coconut bran in a weight ratio of 3:(1-1.5); culturing on a pressure lifting seedling culture bed capable of automatically controlling temperature, controlling moisture and controlling light; controlling parameters of air humidity to 85%, medium moisture content to 50% and illumination intensity to 1500lux; and after one month, causing the cutting wood to form an integral plant, wherein the cuttage survival rate is 80-85%. The method has the advantages of scientific seedling culture facility, high propagation speed, large seedling culture amount, short seedling culture time, high afforestation survival rate, low wood culture cost, good economic benefit and social benefit and the like, offspring can keep the excellent hereditary character of a stock plant, and the asexual seedling culture of camphora fine variety is achieved.

The invention relates to a tunnel half-shading green branch cuttage and propagation method of large reed-leaf cherries and Teng county red cherries, comprising the following steps: making a cuttage bed by using sandy loam as a ground substance ; making a tunnel by using an agricultural film, thin bamboo poles or mocks; covering the film of the tunnel by using a sun-shading net for shading; selecting cherries in winter and spring-heated greenhouses as cutting seed trees, wherein one cutting wood is three nodes having four buds about 10 to 15 cm in general, the distance from apical buds to pruning wounds is from 0.5 to 1cm, 1 / 2 to 1 / 3 of leaves are reserved on a top leaf, and the roots are cut into a shape of a horse ear; putting the cutting wood into a 800-time carbendazol solution for sterilization and moisture preservation after being cut; watering the slotter by using the carbendazol solution for primary soil sterilization and then punching holes by using small bamboo sticks when in cuttage; fully watering by using the carbendazol solution after cuttage, wherein the humidity in the tunnel is kept more than 85%; hardening off seedlings when finding the growth of new leaves in 26th day after cuttage and then uncovering the film for removing the tunnel after a week; shifting to the normal nursery management after uncovering the tunnel, i.e. weeding, watering, fertilizing, preventing and curing plant diseases and insect pests and the like. The invention has the advantages of high nursery stock breeding rate, high transplanting survival rate, no crown gall, vigorous growth, early fruiting, easy fertility and easy popularization.

The invention discloses a giant salamander imitative-ecological taming and breeding method which is characterized in that an imitative-ecological taming and breeding technology is used, an environment suitable for giant salamander survival and growing is established, damage on giant salamanders from natural enemies and various natural disasters is avoided, taming and breeding pools suitable for giant salamanders in different ages are established, a staging taming and breeding technology is used, damage between giant salamander individuals is lowered to the maximum degree, meanwhile, the technology of natural egg laying, natural fertilization and artificial incubation is used, and the breeding emergence rate of the giant salamanders is improved. In a giant salamander parent cultivating stage, optimized fodder with added Chinese herbal medicine which can promote gonad maturing is fed, breeding speed is accelerated, and economic benefit is improved.

The invention provides a quick propagation seedling-breeding method for reducing vitrifaction in multiple times of subculture of a rosaceous plant. The method comprises disinfection of explants, induction of buds, sub propagation and rooting induction. The method is characterized in that: in the sub propagation process, test tube seedlings are subjected to rejuvenation culture alternately, and then vigorous test tube seedlings with height of 1 to 3 centimeters are selected and transferred into a rooting culture medium for induced rooting so as to obtain complete test tube plants, wherein a starting culture medium without containing any exogenous plant hormone for rejuvenation is adopted in the rejuvenation culture. The method is quick, efficient, low in cost and convenient for popularization, has good genetic stability and low vitrifaction ratio in the culture process, keeps the propagation coefficient in a high level, and is suitable for tissue culture and quick propagation of the rosaceous plant.