The invention provides a refined cultivation method of medicinal perilla frutescens. The refined cultivation method sequentially comprises seedbed site selection, making of a seedling-raising shed, floating seedling-raising, transplanting, and leaf harvesting and treatment, wherein the floating seedling-raising comprises the following steps: S1, constructing a seedling-raising pond; S2, making a seedling-raising tray, wherein the seedling-raising tray comprises an outer frame and seedling-raising holes, the outer frame is a hollow concentric-square-shaped frame, the upper side of the side wallof the outer frame is provided with first through holes communicating with the outside world and the inside of the outer frame, the seedling-raising holes are uniformly arranged inside the outer frame, and the adjacent seedling holes are separated by crisscross partitions; S3, preparing a seedling-raising substrate; S4, filling the substrate into the seedling-raising tray; S5, performing sowing;S6, putting the seedling-raising tray into the seedling-raising pond; S7, managing the seedling-raising shed; and S8, performing seedling-lifting. The method is scientific and reasonable, seedling-lifting is convenient and fast in the seedling-raising process, seedlings are not easily damaged, the integrity of the seedlings is good, a survival rate of the seedlings is high, harvested leaves are large, and the yield is high.