The invention discloses a vegetable seedling substrate. A substrate raw material comprises, by weight, waste fungi bags, perlite, remote mountain humus soil, puffed cow dung, pine tree barks, and a Chinese medicine residue; the Chinese medicine residue is a mixture including pine needles, Radix stemonae, Eupatorium adenophora, Dendrobiumnobile, liquorices, and Codonopsispilosula, wherein the mixture is obtained through high-temperature aerobic fermentation processing. The three-phase equilibrium (solids, liquid, and air) optimum environment which can ensure growth and development of plant roots and is required by vegetables, can be established through the specific substrate raw material which has good physical and chemical properties by means of the optimum proportion; the soil is fertile and the cost is low by adoption of the remote mountain humus soil; the Chinese medicine residue including ample organic matters and ample inorganic matters, and nitrogen, phosphorus, and potassium which are required by plant growth, can improve the fertilizer efficiency of the seedling substrate and can kill bacteria; vermiculite and the perlite can change the permeability and the volume-weight of the seedling substrate, can absorb effective constituents of the Chinese medicine residue, and can play the effect of sustained release.