This invention relates to the field of
plant seedling technology, specifically to a method for improving the
survival rate of
Melaleuca alternifolia seedlings, comprising: S1, disinfecting
Melaleuca alternifolia seeds; S2, preparing a seed suspension using the
Melaleuca alternifolia seeds obtained in step S1; S3, inoculating the seeds soaked in step S2 into a
germination medium for
germination culture, wherein the
germination medium comprises 1 / 2 MS, 0.1-0.4 mg / L BA, 0.01-0.1 mg / L NAA, 20-30 mg / L GSH, 0.3-0.7 mg / L
triacontanol, 0.1-0.5 mg / L
betaine, and 0.1-0.3 mg / L
acetic acid. mg / L
sodium molybdate, 20-30 g / L
sucrose, 1-5 g /
L glucose, 4.5-6 g / L
agar powder; S4, when the seeds from step S3 germinate into seedlings, the seedlings are transplanted from the germination medium to a
seedling strengthening medium for the first and second stages of
seedling strengthening culture; S5, hardening off,
transplanting, and seedling management. Compared with the prior art, this invention uses a unique disinfection and germination / strengthening culture method to solve the problems of difficult disinfection of very small seeds of *
Melaleuca alternifolia*, incomplete disinfection, or low germination rate due to seed poisoning after disinfection, thereby improving seed germination rate and seedling transplant
survival rate, and shortening seedling time.