81 results about "Seed dormancy" patented technology

The invention relates to the field of alfalfa planting, in particular to a synthetic flora for breaking dormancy of alfalfa seeds and promoting growth of seedlings and application of the synthetic flora. The invention provides a strain of sinorhizobium meliloti NFB5, the preservation number is CGMCC NO.33618, and the sinorhizobium meliloti NFB5 can break dormancy of alfalfa seeds and promote growth of alfalfa seedlings and root systems. According to the method, sinorhizobium meliloti NFB5 is compounded with one or two of screened salt-tolerant brevibacillus brevis EB3 and bacillus PS06 to obtain a synthetic flora, and alfalfa seeds are soaked in a bacterial solution containing the synthetic flora, so that the number of hard seeds can be remarkably reduced, the germination rate and germination potential of the seeds can be remarkably improved, and the germination rate of the seeds can be remarkably increased. Therefore, the synthetic flora can well break the dormancy of the alfalfa seeds, does not cause loss to the seeds, and does not reduce the quality of the seeds. In addition, the synthetic flora also has a significant growth promoting effect, and can significantly improve the plant height, root length, stem weight and root weight of seedlings.

The invention provides a sand penis seedling cultivation method, and belongs to the technical field of sand fixation plant cultivation. The sand penis seed seedling cultivation method comprises the steps of seed collection, seed dormancy breaking, sowing, seedling raising, seedling hardening, row belt type sand barrier arrangement, target sand dune pretreatment, transplanting and the like. The invention solves the problem of lack of the sand whip seedling and artificial cultivation technology on the moving dune at present. According to the method, the seed collection cost is only 4.8-20.0% of the traditional collection cost. According to the method for cultivating the sand penis seedlings, the germination rate is 95% or above, the emergence rate is 85% or above, the average height of the sand penis on a moving dune is 30-35 cm in the same year of transplanting, and the preservation rate can reach 70% or above. The sand penis seedling cultivation method provided by the invention makes the most important step for research and development of sand penis live sand barriers.

The invention discloses a preparation technology of a special cistanche inoculation block with a seed dormancy breaking self-induction function by combining a cistanche seed dormancy breaking inducer and hydrogel, and relates to the technical field of cistanche cultivation. The specific technical process is as follows: preparation of a dormancy breaking inducer solution, preparation of a hydrogel block containing the dormancy breaking inducer and cistanche seeds, and preparation of an inoculation block. According to the method, gibberellin and cytokinin are used as dormancy breaking inducers, and a yeast aqueous extract is used as an auxiliary agent. The problems of seed dormancy breaking, germination promoting and water retention inoculation are effectively solved, seed dormancy breaking does not need to be conducted in advance before inoculation, meanwhile, hydrogel has high water absorption and water retention capacity, the problem that dormancy breaking seeds are inactivated due to the fact that the dormancy breaking seeds cannot germinate and parasitize in time after being inoculated due to water shortage is effectively solved, and the survival rate of the seeds is increased. The inoculation efficiency is improved to 75% or above from about 20% of existing inoculation paper inoculation, and the method has a good popularization prospect and has very important significance in promoting the industrial development of cistanche deserticola.

The invention discloses a podophyllum hexandrum callus rapid proliferation method, and belongs to the technical field of plant tissue culture, the podophyllum hexandrum seed dormancy is broken through a sand storage method, the seeds are soaked in distilled water and a gibberellin solution and then are fully and uniformly mixed with river sand, after storage and germination acceleration, the seeds are sown in soil added with an equal amount of matrix, and podophyllum hexandrum seedlings are obtained through culture; fresh leaves of podophyllum hexandrum seedlings are taken as explants, the explants are disinfected and cut into small pieces, the small pieces are cultured on an MS culture medium added with hormone for 30 d to induce callus, the induced callus is cultured and proliferated at the optimal temperature of 15 DEG C, and the callus is inoculated to the same fresh culture medium after being cultured for 20 d to prevent browning, so that the high-quality callus is obtained. And then replacing a fresh culture medium every 20 days to realize rapid and mass proliferation of the podophyllum hexandrum callus. Through the method, a large amount of high-quality podophyllum hexandrum callus can be rapidly obtained, and sufficient materials are provided for podophyllotoxin extraction and medicinal value development.

The invention discloses a rice seed dormancy gene OsPHS1 and application of a protein encoded by the gene OsPHS1, and belongs to the technical field of plant genetic engineering, and the gene and the protein encoded by the gene are used for regulating the dormancy of rice seeds. The nucleotide sequence of the gene is shown as Seq ID No: 1, the CDS sequence is shown as Seq ID No: 2, and the coded protein sequence is shown as Seq ID No: 3. The function of the rice seed dormancy gene OsPHS1 is verified by cloning and identifying the rice seed dormancy gene OsPHS1, and research finds that the dormancy of rice seeds can be remarkably reduced by knocking out or mutating the OsPHS1 gene, and the dormancy of the rice seeds can be improved to a certain extent by overexpressing the gene; the invention plays an important role in developing rice varieties with moderate dormancy.

The invention belongs to the technical field of plant seedling raising, and relates to a method for breaking dormancy of iris ensata thunb seeds and accelerating germination, which comprises the following steps: (1) soaking disinfected iris ensata thunb seeds in hydrogen peroxide with the concentration of 3-7wt% for 15-30min; and (2) at 25-30 DEG C, soaking with a mixed solution of gibberellin 3 and indoleacetic acid, and accelerating germination. Hard seed coats are broken through H2O2 soaking treatment, seed coat obstacles and physiological dormancy of the iris ensata thunb seeds are broken through combination of a GA3 + IAA seed soaking mode and fine sand covering germination acceleration, hormones needed by seed germination are supplemented, germination inhibitors existing in the seeds are inhibited, dormancy of the iris ensata thunb seeds is comprehensively relieved, the germination rate of the iris ensata thunb seeds is remarkably increased, and the germination rate of the iris ensata thunb seeds is increased. The germination rate is increased to 80% or above, the germination time can be further shortened, and technical support is provided for large-scale production and application of iris ensata thunb.

The application discloses a kind of based on mechanical peeling cold storage germination polyphylla prataeshis greenhouse seedling technique, belong to the field of breeding technology.The application uses hexagonal drum screening machine to carry out mechanical peeling to polyphylla prataeshis seed, and stores kernel under the condition of artificial temperature control, humidity control, and the strength is easy to grasp, effectively slows down seed mildew phenomenon, and seed embryo is intact, the seedling method of the patent has the advantages that seed embryo disease infection rate is low (6% or less), seed dormancy time is short (5 months), seedling time is short, germination rate is high (98%), seed treatment capacity is large (one batch can handle 50 jin), operation difficulty is low, growth speed is fast, seedling quality is high, and it is favorable to improve the success rate of large-scale breeding of polyphylla prataeshis.

The invention discloses a bletilla striata seedling raising method which comprises the following steps: selecting and pretreating seeds, selecting healthy and mature bletilla striata seeds, ensuring that the seeds are free of plant diseases and insect pests and seed coats are complete, soaking the screened mature seeds in warm water of 30 DEG C for 4 hours, promoting the seed coats to be softened, breaking seed dormancy, and raising the seedlings. Transferring the soaked seeds into a low-temperature environment of 4 DEG C for stratification treatment, wherein the stratification time is 15 days. A stratification medium is wet vermiculite, the humidity is kept at about 60%, and after stratification treatment, the seeds are soaked in a 0.05% gibberellin solution for 30 minutes to promote germination of germs. The invention relates to the technical field of plant seedling cultivation, in particular to a bletilla striata seedling cultivation method capable of designing differentiated pretreatment processes according to different propagation materials, achieving accurate management of temperature and humidity, illumination and fertility and improving the bletilla striata seedling cultivation effect.

The present invention relates to the field of ecosystem restoration technology, and in particular to a seed capsule suitable for arid and semi-arid areas, and a preparation method and application thereof. The seed capsule includes a cylindrical seed core, a spacer layer and a protective layer from the inside to the outside, the seed core includes pioneer plant grass seeds, potassium humate, chitosan, camphor, probiotic spore powder and sand, the spacer layer is provided with pioneer plant grass seeds different from those on the seed core, the seed capsule is a triangular prism, the spacer layer is provided with three groups of seedling holes along the circumference of the seed core, the seedling holes are located inside the side of the triangular prism, and the protective layer includes clay, cattle and sheep manure, biochar, fruit crushed material, water retaining agent, compound fertilizer and guar gum. The seed capsule provided by the present invention has the properties of salt resistance, drought resistance and cold resistance, and can withstand harsh natural environments, wherein the seed core is relatively dry, can create a long-term seed dormancy environment, and has a small requirement for the application time period.

This invention discloses a herbicidal composition containing propyzoxystrobin and chlorpyrifos, and its application, belonging to the field of pesticide technology. The active ingredients of this herbicidal composition are propyzoxystrobin and chlorpyrifos, with a weight ratio of propyzoxystrobin to chlorpyrifos of 1:5 to 5:1. Through the synergistic effect of the two active ingredients, this herbicidal composition addresses the seed dormancy and germination characteristics and resistance issues of black nightshade in pumpkin fields, achieving a synergistic control effect of "rapid-acting + long-lasting" and "closed + foliar" action. The control efficiency can reach over 80%, with an optimal ratio reaching as high as 93.1%. It also significantly increases pumpkin yield without adversely affecting pumpkin growth, solving the technical problems of the lack of dedicated control agents for black nightshade in pumpkin fields and the strong resistance of conventional agents in existing technologies.

This invention discloses a method for breaking seed dormancy in *Palmeria nivea* seeds. This invention rapidly breaks seed dormancy through minimally invasive seed coat incision, combined with a fungicide-treated seedling tray device. This allows for seed germination control within 25 days from seed collection, with a rooting rate of 92.4% and a seedling survival rate of 83.69% after 45 days. After 45 days of cultivation in the fungicide-treated seedling tray, without the need for water or fertilizer, the average plant height is 8.46 cm, branch nodes reach 3 nodes, and root system length reaches 5.89 cm. This invention achieves rapid breaking of seed dormancy in *Palmeria nivea*, efficiently promotes seed germination and seedling growth, and reduces seedling management time. It achieves the goals of rapid seed germination and efficient seedling growth, providing a strategy for the protection and population expansion of the endangered plant *Palmeria nivea*.

The useful solution describes a process for breaking the dormancy of Vietnamese ginseng (Panax vietnamensis) seeds using plasma technology, which includes the following steps: a) seed collection and preliminary processing; b) treatment with plasma-activated water (PAW); c) treatment with gaseous plasma; and d) breaking the dormancy of Vietnamese ginseng seeds. In addition, the solution also describes a process for producing Vietnamese ginseng (Panax vietnamensis) seedlings, which includes the following steps: a) seed treatment; b) sowing treated seeds into a substrate; and c) seedling care. The process described in the solution stimulates development and completes embryo structure, shortens seed dormancy, increases germination rates, and allows for proactive seedling production of Vietnamese ginseng.

The present invention provides a Lonicera japonica EVM0028813 gene and its use in regulating plant seed dormancy. The nucleotide sequence of the Lonicera japonica EVM0028813 gene is shown in SEQ ID NO: 1. The amino acid sequence of the Lonicera japonica protein is shown in SEQ ID NO: 2. Plant seeds overexpressing the Lonicera japonica EVM0028813 gene significantly extend dormancy, preventing the problem of pre-harvest germination.

The invention is suitable for the field of traditional Chinese medicinal material planting, and provides a long-stem gynostemma pentaphyllum seed seedling raising method which comprises the following steps: seed harvesting, seed storage, seed cleaning, seed freezing, seed washing, seed propagation and seedling raising management. According to the method, dormancy limitation is broken through, the germination rate is remarkably increased, through cooperative treatment of freezing for 12 h at the temperature of-18 DEG C and normal-temperature flowing water flushing for 24 h, dormancy of the long-stem gynostemma pentaphylla seeds is thoroughly broken, the germination rate can reach 95% or above, the core problem that the germination rate of conventional sowing is zero is solved, and technical guarantee is provided for large-scale seedling raising; the method is simple and easy to operate and suitable for industrial popularization; the seedling quality is excellent, and the planting benefit is improved; wild resources are protected, and sustainable development is promoted; the technical system is scientific, and seed activity is guaranteed.

This invention relates to the fields of molecular biology and biotechnology, and provides the ZmCNX2 gene, a cofactor 2 protein gene for nitrate reductase and xanthine dehydrogenase in maize, and its applications. The nucleotide sequence of the ZmCNX2 gene is shown in SEQ ID NO:1, and the amino acid sequence of the cofactor 2 protein encoded by the ZmCNX2 gene for nitrate reductase and xanthine dehydrogenase is shown in SEQ ID NO:2. This invention found that mutants of the ZmCNX2 gene in maize exhibit a pre-harvest germination phenotype; compared with wild-type Arabidopsis seeds, the germination of transgenic Arabidopsis seeds was significantly delayed. These results indicate that deficiency of the ZmCNX2 gene can lead to maize seeds lacking dormancy and germinating directly on the ear, while overexpression of the ZmCNX2 gene in Arabidopsis can delay seed germination, further verifying the central role of this gene in the regulation of seed dormancy and germination.

The invention provides application of a rice JAZ protein gene OsJAZ12 in pre-harvest sprouting resistance, a nucleotide sequence of the rice JAZ protein gene OsJAZ12 is shown in a sequence table SEQ ID NO.1, an amino acid sequence of the rice JAZ protein gene OsJAZ12 is shown in a sequence table SEQ ID NO.2, the rice JAZ protein gene OsJAZ12 mutant can be applied to rice pre-harvest sprouting resistance, and the possibility of sprouting in the rice seed dormancy period is reduced.

The invention relates to the technical field of wheat genetic breeding, and discloses a wheat seed dormancy gene TaCaM-3A and application thereof. The full-length sequence of the TaCaM-3A gene is as shown in SEQ NO. 1. The gene TaCaM-3A for negative regulation of seed dormancy and pre-harvest germination resistance is identified, the pre-harvest germination resistance of wheat varieties can be remarkably improved due to gene function deletion, and the grain length, the grain width and the yield of a single plant are all increased. The gene has the potential of improving wheat pre-harvest germination resistance and yield at the same time, and has great breeding application value in wheat pre-harvest germination resistance breeding.

The invention discloses a wild lespedeza seed dormancy breaking and germination accelerating method, and belongs to the technical field of plant seed breeding. The method comprises the steps of physical skin breaking, surface cleaning and disinfection, warm water seed soaking, chemical dormancy breaking, gradient germination accelerating, batch sowing and intelligent water and fertilizer management. Aiming at the problems of low natural germination rate, irregular seedling emergence and the like caused by double obstacles of hard seed coats and shallow physiological dormancy of wild lespedeza seeds, the method synchronously breaks the double dormancy obstacles through a multi-stage synergistic mechanism of constructing a moisture permeation channel through physical coat breaking, breaking dormancy through chemical dormancy breaking and simulating environmental signals through gradient germination acceleration, and the germination rate of the wild lespedeza seeds is improved. The seed germination rate and the seedling emergence uniformity are effectively improved, and mildewing is inhibited. The method is stable in process, high in operability and suitable for large-scale seedling raising of the wild lespedeza, and reliable technical support is provided for ecological restoration engineering.

The invention relates to the technical field of ecological management, and discloses a method for planting suaeda salsa in saline-alkali soil, which comprises the following steps: seed pretreatment: freezing suaeda salsa seeds in a low-temperature environment of-5 DEG C to 0 DEG C for 12-24 hours, then quickly transferring the seeds to an environment of 25 DEG C to 30 DEG C, unfreezing and activating, and taking out the seeds; then putting into a mixed solution containing 0.5%-1% of biological enzyme and 0.3%-0.5% of plant hormone, and soaking for 8-12 hours; soil improvement: paving a layer of mixture of organic fertilizer and saline-alkali tolerant microbial agent with the thickness of 5-10cm on the surface of the saline-alkali soil. Through an innovative seed pretreatment method, low-temperature freezing and unfreezing activation are combined with biological enzyme and plant hormone solution soaking, seed dormancy is broken, internal physiological metabolism of seeds is activated, and the seeds germinate more quickly and neatly. The organic fertilizer and the saline-alkaline tolerant microbial agent in scientific proportion are matched for soil improvement, so that the saline-alkaline degree of the soil is effectively reduced, and the microbial community structure of the soil is improved.

The invention relates to the technical field of polygonatum kingianum seed treatment, in particular to a method for increasing the germination rate of polygonatum kingianum seeds, which comprises the following steps: S1, selecting polygonatum kingianum seeds; s2, the treated polygonatum kingianum seeds are subjected to low-temperature treatment, surface disinfection and moisture absorption; s3, soaking the polygonatum kingianum seeds in water at the water temperature of 25-60 DEG C for 20 minutes, cooling to room temperature after soaking, disinfecting, washing with sterile water, and absorbing moisture; s4, taking a tissue culture bottle, cleaning, drying, putting multiple layers of filter paper, sterilizing, cooling to room temperature, dropwise adding sterile water, infiltrating the filter paper in the tissue culture bottle, putting the polygonatum kingianum seeds on the filter paper in the tissue culture bottle, and covering the tissue culture bottle with a bottle cover; s5, putting the tissue culture bottle into an artificial climate box, and culturing for 12-14 days in a dark place at the temperature of 25 DEG C to obtain seedlings; according to the method, seed dormancy is effectively broken, so that seeds harvested in the season can rapidly germinate and be sown, and the seedling rate of polygonatum kingianum seeds is increased.

Provided are a gene for regulating seed dormancy and germination of a gramineae plant and the use thereof. Seed dormancy and seed germination in plants are regulated by means of regulating the expression or activity of a plant ERF04 gene or a protein encoded thereby. The deletion of the ERF04 gene leads to an increase in seed dormancy and reduces the pre-harvest sprouting phenomenon. By means of the provided method, a plant strain with enhanced dormancy can be obtained, and the pre-harvest sprouting resistance thereof can be effectively improved, providing a new approach for improving the seed dormancy and reducing the pre-harvest sprouting in plants.

The present application belongs to the technical field of seed germination, and relates to a kind of seed dormancy breaking and germination method of Rhizoma Polygonati, which comprises the following steps: after seed disinfection and activation, ultrasonic-magnetic field treatment is carried out; mixed with composite growth regulator, vacuum-normal pressure cycle treatment is carried out; then mixed with stratification matrix, variable temperature stratification treatment is carried out; then transferred into germination substrate, high humidity treatment, photoperiod germination and spraying germination nutrient solution are carried out in turn; low temperature passivation synchronization treatment is carried out in the middle of germination, and then the germination conditions are restored until the seed germination. The present application effectively breaks the dormancy of Rhizoma Polygonati seed by a series of means such as ultrasonic-magnetic field synergistic wall breaking, vacuum impregnation multi-target hormone induction, programmed variable temperature stratification, low temperature synchronization regulation and light temperature and humidity cultivation coupling germination, significantly shortens the germination period, improves the germination uniformity and seedling robustness, and is suitable for large-scale and standardized seedling raising of Rhizoma Polygonati seed.

The utility model is suitable for the technical field of traditional Chinese medicinal material planting, and provides traditional Chinese medicinal material seed dormancy breaking treatment equipment. The warm water tank is fixedly mounted on one side of the cold water tank; the supporting rods are fixedly mounted on the cold water tank and the warm water tank; the same treatment box is fixedly mounted on the plurality of supporting rods; the box cover is hinged to the treatment box; the temperature adjusting mechanism is arranged on the cold water tank and the warm water tank and is used for adjusting the water temperature in the cold water tank and the warm water tank; and the circulating temperature control mechanism is mounted on the treatment box and is used for periodically and cyclically adjusting the soil temperature. The traditional Chinese medicinal material seed dormancy breaking treatment equipment provided by the scheme can simulate the natural soil temperature change condition to periodically adjust the soil temperature, has an illumination regulation and control function, and can accelerate the breaking of traditional Chinese medicinal material seed dormancy.

The invention relates to the technical field of plant seed planting, in particular to a rice seed dormancy breaking method which specifically comprises the following steps: 1) seed selection: selecting rice seeds which are stored in a seed cellar at 4 DEG C and are in a dormant state; (2) soaking the rice seeds selected in the step (1) in a dormancy breaking promoting agent for 24 hours, and cleaning with clean water; and 3) carrying out a standard germination experiment at a constant temperature of 28 DEG C, and observing and recording the germination condition. The method has the advantages of being reasonable in step design, convenient to operate and remarkable in dormancy breaking effect on the rice seeds. The method can be conveniently applied to actual production, and the utilization rate and planting benefits of the seeds are improved.

The invention relates to the technical field of seed dormancy, and particularly discloses a vicia sativa seed dormancy breaking device which comprises a material breaking barrel, a peel breaking device is fixedly connected to the center of the inner wall of the material breaking barrel, a material guide pipe is fixedly connected to the top of the material breaking barrel, and an awakening assembly is fixedly connected to the side face of the material breaking barrel. The bottom of the crushing barrel is fixedly connected with a collecting assembly, the bottom of the collecting assembly is fixedly connected with a soaking pool, the bottom of the collecting assembly is fixedly connected with the bottom of the inner wall of the soaking pool, the skin crushing device comprises a rotating barrel, the side face of the rotating barrel is fixedly connected with a fixing strip, and the side face of the fixing strip is fixedly connected with a scraping knife. A transmission shaft is fixedly connected to the top of the inner wall of the rotating cylinder, the side face of the transmission shaft is sleeved with and slidably connected with a meshing assembly, and a spring is fixedly connected to the top of the meshing assembly.

The utility model discloses a simple seedling raising suite for breaking the dormancy of epimedium seeds, which comprises a lamination basin, a scraper blade and a sealing belt, the lamination basin is a cuboid groove with an opening at the top, the side wall of the long edge of the lamination basin is used as a first side wall, and the side wall of the wide edge of the lamination basin is used as a second side wall; a first reinforcing rib and a second reinforcing rib are arranged on the inner wall of each first side wall, the two first reinforcing ribs correspond to each other, the two second reinforcing ribs correspond to each other, and the top surfaces of the first reinforcing ribs and the top surfaces of the second reinforcing ribs are parallel to the inner bottom surface; the two ends of the first reinforcing rib are connected with the inner walls of the two second side walls respectively, and the distance between each end of the second reinforcing rib and the inner wall of the opposite second side wall is larger than the width of the scraper blade; the length of the scraper is larger than the distance between the two first reinforcing ribs and the distance between the two second reinforcing ribs and smaller than the distance between the inner walls of the two first side walls. The device has the advantages that the dormancy of the epimedium seeds can be broken, and large-scale seedling culture can be realized.

The utility model discloses hoof leaf ligusticum sinense oliver seed dormancy breaking treatment device, especially in the technical field of breeding, including the treatment box, a plurality of positioning holes are seted up in the one side of treatment box, is equipped with the layered positioning treatment spare in the one side of positioning hole, and the layered positioning treatment spare includes: a plurality of seed disc all are arranged in the one side of positioning hole, and the inner wall of every seed disc all sets up a plurality of hole bodies, the handle is connected in the one side of seed disc, and a plurality of shunt pipes all are inserted in the inside of treatment box, and the outer wall of every shunt pipe all is fixed and is communicated with a plurality of atomizing nozzles. The utility model discloses layered positioning treatment spare, and the treatment box is equipped with a plurality of positioning holes and a plurality of seed discs alignment, and the seed disc is inserted into the positioning hole by pushing the handle, realizes the layered positioning lower spray treatment, avoids the layered deviation, and improves the breeding efficiency.

The invention discloses a method for breaking dormancy of epimedium villosum seeds and realizing efficient germination. The method comprises the following steps: performing seed disinfection pretreatment, soaking with 8-12mg / L FL and / or 150-250mg / L GA3 solution, performing stacking treatment in an environment of 4-10 DEG C for 60-90 days, and then germinating. The invention reveals that the dormancy breaking has two relatively independent passages of morphological after-ripening and biologic unlocking, GA3 dominates embryo morphogenesis, and FL efficiently relieves physiological dormancy. According to the preferable scheme, the germination rate is increased to 69.22% from 1.33% in a natural state through soaking for 6 hours in 10 mg / L FL and dark stratification for 90 days at the temperature of 4 DEG C. According to the method, the seedling raising period is remarkably shortened, the bottleneck that the sexual reproduction efficiency is extremely low in artificial cultivation of epimedium villosum is effectively solved, and key technical support is provided for artificial seedling raising of the species and sustainable utilization of resources.

The present invention provides a management method for promoting the germination of polygonatum cyrtonema seeds, and relates to the technical field of polygonatum cyrtonema seeds treatment. The management method mainly comprises the steps of soaking berries picked in the current season in ice water, blanching in boiling water and soaking in warm water to obtain seeds, preparing nutrients by mixing menthol, salicylic acid, madecassoside, xylitol and other substances with saline, high-pressure soaking and ultrasonic treatment of the seeds after the seeds are sterilized, germination at constant temperature and humidity, sowing and the like. The present invention overcomes the shortcomings of the prior art, effectively breaks the dormancy of seeds so that the seeds harvested in the current season can be directly germinated and sown, and promotes subsequent emergence, thereby comprehensively improving the efficiency of polygonatum sowing.

The invention relates to the technical field of plant seed treatment, in particular to a variable-temperature sand storage treatment process for breaking deep dormancy of seeds, which comprises the following steps: step (1) pretreatment: removing impurities from seeds, selecting mature and full seeds with uniform shape and size, soaking and scrubbing the seeds with clear water, and drying the seeds; naturally air-drying for later use; (2) disinfection: disinfecting with sodium hypochlorite, washing, and drying in the shade at an indoor ventilated place to obtain disinfected seeds; step (3) seed soaking; step (4) preparation: adjusting the humidity of the clean river sand by using sterilization distillation to obtain wet river sand; (5) sand storage; and (6) temperature changing: performing indoor hot storage, transition, cold storage and temperature returning, and checking the white exposure condition. According to the variable-temperature sand storage treatment process for breaking the deep dormancy of the seeds, the variable-temperature sand storage treatment process is provided, the common risks of mildewing, rotting and uneven germination in the traditional lamination process are effectively reduced through accurate temperature, humidity, time, mechanical scratching and transition stages, the germination rate of the seeds is increased, and the application prospect is good.