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586 results about "Seed dormancy" patented technology

Seed dormancy is an evolutionary adaptation that prevents seeds from germinating during unsuitable ecological conditions that would typically lead to a low probability of seedling survival. Dormant seed do not germinate in a specified period of time under a combination of environmental factors that are normally conducive to the germination of non-dormant seeds.

Encapsulated phase change materials in seed coatings

The present invention is directed to improved seed coatings which facilitate fall or early spring planting while maintaining seed dormancy until soil temperatures are appropriate for successful germination. The improved seed coatings contain encapsulated phase change materials within a polymeric shell which preserve the dormancy of the seed during early planting by slowing the rate at which the seed temperature rises in the event of a temperature spike thus preventing premature germination. The encapsulated phase change material is a material characterized by a solid / liquid or liquid / solid phase change which occurs at a temperature which ranges from about −5 to about 20° C., preferably between about 0 to about 19° C., most preferably between about 5 to about 15° C. The solid / liquid or liquid / solid phase change is further characterized by an effective enthalpy of fusion / crystallization for the solid-liquid / liquid-solid phase change equal to or greater than 20 J / g when determined by Differential Scanning Calorimetry.
Owner:CIBA CORP

Method for effectively breaking dormancy of Paris polyphylla seeds

The invention discloses a method for effectively breaking the dormancy of Paris polyphylla seeds. By adopting a treatment way of combining hydrogen peroxide and the Gibberellins solution with suitable concentrations, the dormancy of the Paris polyphylla seeds is effectively broken, the after ripening period of the seeds is shortened, the germination time of about 2 years for the Paris polyphylla seeds in natural conditions is shortened to about 3 months, and the cultivation time of the Paris polyphylla seeds is greatly shortened.
Owner:SICHUAN AGRI UNIV

Production method for paraffin section of paeonia lactiflora mature embryo

The invention discloses a production method for a paraffin section of a paeonia lactiflora mature embryo. The production method comprises the following steps: drawing materials and fixing: stripping a seed coat, cutting a seed, immersing the seed top part containing the embryo or part endosperm into a FAA stationary liquid, and fixing for more than 24 hours; dehydrating: washing in ethyl alcohol with the concentration of 70% for 3 to 5 times, and performing gradient dehydration from the ethyl alcohol with the concentration of 70%; performing hyalinization: carrying out five level hyalinization by using dimethyl benzene as a transparent reagent; waxing and embedding: pouring out one half volume of dimethyl benzene, adding wax crumbs in a bottle until saturation, staying overnight at the temperature of 40 DEG C; then changing a wax liquid every two hours for three times per day, repeating for three days, and carrying out conventional paraffin embedding; slicing; flattening and bonding: flattening a linoleum tape on an iron plate of a water bath kettle at the constant temperature of 50 DEG C, dewaxing, rehydrating, dyeing through safranine and fast green and performing hyalinization; mounting. According to the invention, the paeonia lactiflora mature embryo paraffin section of which the tissue structure is integrated and clear can be obtained, so that accurate technical support can be provided for researching paeonia lactiflora seed dormancy and germination.
Owner:SHENYANG AGRI UNIV

Method for cultivating seedlings of oil peonies

The invention relates to a method for cultivating seedlings of oil peonies, and belongs to the field of agricultural seedling cultivation. The method comprises the following steps: selecting a nursery; performing weeding in advance; performing deep ploughing and tedding; performing soil preparation and making furrow ridges; harvesting seeds; processing the harvested seeds; sorting the processed seeds; processing the sorted seeds before cultivation; sowing the processed seeds; managing seedlings; managing nursery stocks. The characteristics of soil are changed by the method of combining farm manure with a chemical fertilizer. The seeds for sowing are harvested at the best harvesting time; the seeds to be cultivated are processed by gibberellin, ethyl alcohol, sodium p(dimethylamino) benzenediazo sulfonate solution, carbendazim and plant ash, and a culture solution is generated during the cultivation of onions, so that the seed dormancy is broken, the germinating time is advanced, and the germination rate is increased. Diseases are prevented during cultivation, and different medicaments are sprayed in different seasons so as to prevent diseases, so that the seedling survival percent is increased. After being cultivated by the method disclosed by the invention, the nursery stocks are thick in plants, the seedling survival percent is high, the quality is greatly improved, after the nursery stocks are planted, the seed setting rate is high, and the nursery stocks cultivated by the method disclosed by the invention are good varieties for the oil peonies.
Owner:TONGLING FENGWEI AGRI TECH CO LTD

Watermelon seed germination acceleration method

The invention provides a watermelon seed germination acceleration method. The watermelon seed germination acceleration method comprises the steps of selecting seeds, soaking the seeds with a seed soaking agent, soaking the seeds with a microwave nutrient solution, magnetizing the seeds in a magnetizer, storing the seeds in a refrigerator at varying temperature, conducting seed dressing with rare earth and accelerating germination. According to the watermelon seed germination acceleration method, seed dormancy is broken quickly, the germination rate is increased, root systems of seedlings and watermelon vines are flourishing after seeds processed through the watermelon seed germination acceleration method are planted, the number of plant diseases during the growing period is small, watermelons are high in drought resistance and cold resistance, microelements needed by plants are supplemented, and the quality of the watermelons is improved.
Owner:MAANSHAN LVFENG SEED IND CO LTD

Rapid rhizoma polygonati propagation technology method

The invention provides a rapid rhizoma polygonati propagation technology method. The method comprises the following steps: breaking seed dormancy by changing temperature treatment and hormone treatment, performing induction in vitro to culture callus by utilizing seeds subjected to dormancy breakage as materials, performing differentiation culture and root induction, and performing vegetative propagation on rhizoma polygonati seedlings. By adoption of the technology, the rhizoma polygonati propagation factor can be greatly improved, the production period is shortened, the sowing quantity is reduced, and the production cost is reduced.
Owner:湖南宏邦生物科技有限公司

Method for removing seed dormancy of tapiscia sinensis oliv and improving seedling survival rate

The invention discloses a method for removing the seed dormancy of tapiscia sinensis oliv and improving the seedling survival rate. The method is mainly realized by the following steps: selection of seeds, disinfection of seeds, ultrasonication, hormone and heterotherm stratification, seed seedling and the like. By means of technology of growth regulator control, heterotherm treatment and the like for breaking the seed dormancy, with the adoption of the growth regulator with appropriate concentration and an appropriate heterotherm treatment medium, the seed germination time of tapiscia sinensis oliv is greatly shortened. Furthermore, with the adoption of appropriate temperature and light treatment as well as an appropriate seedling medium, the survival rate of a nursery-grown plant is further high.
Owner:CHANGSHU JIASHENG AGRI SCI & TECH DEV

Method for breaking seed dormancy of Paris polyphylla var. yunnanensis

The invention relates to a method for breaking the dormancy of the seeds of Paris polyphylla var.yunnanensis, belonging to the technical field of Chinese traditional medicine planting. The method includes the following steps: 1. the mature seeds of the Paris polyphylla var.yunnanensis with red seed coats are collected in September to October every year, the red seed coats are washed away, the seeds are naturally dried in shade at room temperature for 15 days and then are filled into air permeable valve bags for storage; 2. the seeds stored in the bags are taken out at the middle of November, dipped in the water with room temperature for 15 hours, then are taken out and naturally dried in shad at room temperature for 15 hours, and finally are again dipped into the water with room temperature for 15 hours; 3. the processed seeds are directly sowed in soil for sprouting, covered with 2 cm thick soil, and watered in an appropriate state that no water accumulation exists; the seeds in soil sprout in 60 days, and germination rate can reach over 90%. Compared with the existing processing method of controlling temperature and applying gibberellin, the method has the advantages of simple operation, low cost, quick germination, etc., thus the method can be extensively applied to the planting of the traditional Chinese medicine of Paris polyphylla var.yunnanensis.
Owner:YUNNAN UNIV

Germination accelerating method for fructus crataegi seeds

The invention mainly relates to the technical field of planting and discloses a germination accelerating method for fructus crataegi seeds. The method includes: soaking, performing cold storage, drying, accelerating germination and sowing. The method has the advantages that the method is easy to operate and simple, germination accelerating is shorted to 8-9 days, germination rate reaches 86%, the planting efficiency of fructus crataegi is increased evidently, and economic income is increased by 11.7%; the fructus crataegi seeds are soaked by salt water, moisture can fast enter the seeds, seed coat softening is accelerated, and germination time is shortened; the soaked seeds are stored in cold storage liquid to allow nutrition to fast enter the seeds, germ stress resistance is increased, and the seedling survival rate is increased and reaches 93%; the seeds are dried at low temperature, the drying of surface of the seed coat is promoted, and the seed coat cracks to allow the nutrition and moisture to enter the seeds so as to promote the germination; germination accelerating liquid is sprayed during germination accelerating, humidity can be kept while seed dormancy can be broken, antibacterial and pest-killing effects are achieved, and seed germination rate and survival rate are increased.
Owner:阜阳市颍泉区玉寿种植专业合作社

Seed germination acceleration seedling culturing method for polygonatum cyrtonema

The invention provides a seed germination acceleration seedling culturing method for polygonatum cyrtonema. The seed germination acceleration seedling culturing method comprises the steps of conducting fermentation treatment on fruits through valve bags, conducting low-temperature lamination treatment, conducting hormone treatment, breaking seed dormancy, inducing dormant seeds to germinate and grow to seedlings and the like. According to the seed germination acceleration seedling culturing method, by breaking seed dormancy and bud dormancy of polygonatum cyrtonema, the seedling culturing period of polygonatum cyrtonema can be greatly shortened, the production cost is reduced, the propagation coefficient of polygonatum cyrtonema is improved, and the problem that high-quality polygonatum cyrtonema seedlings are in short supply is solved.
Owner:安徽省应用技术研究院

Summer and autumn cultivation method of ginger

The invention belongs to a cultivation method for planting ginger in late summer and early fall and harvesting young ginger in winter. The method comprises the steps of seed dormancy and storage, resuscitation before seed sowing, germination promotion, plastic greenhouse building, seed sowing, daily management and harvest. According to the invention, ginger seeds are stored at dormancy temperature and humidity, thereby enabling the seed ginger to fight against high temperature and germinate in spring and summer; the film and sun shade of the greenhouse are removed during the sowing stage and early stage of growth, thereby preventing rain and reducing soil temperature and humidity; the relative humidity of soil is kept by controlling drip irrigation; the sun shade is removed and the film is added during the middle stage of growth; the greenhouse is provided additionally with a small arc shade and covered with stalks for frost protection and fresh-keeping during the late stage of growth until harvest. The young ginger harvested in winter is large and flavorful. The yield per mu is increased by 1-2 times as compared with greenhouse cultivation. Additionally, the method has the advantages of simple daily management, low production cost, suitability for large-area commercial cultivation, etc.
Owner:陈炳金

Method for breaking Thesium chinense seed dormancy for promoting germination

ActiveCN102057777AEffective dormancyHibernationSeed and root treatmentSeed dormancyThesium chinense
The invention discloses a method for breaking Thesium chinense seed dormancy for promoting germination. The germination stage of Thesium chinense seeds is put ahead greatly by breaking the Thesium chinense seed dormancy by a method of soaking the seeds by using 500 mgL<-1> of GA3 for 24 hours and laminating on wet sand at a low temperature for 150 days. Therefore, the problems that the Thesium chinense seeds are in deep dormancy and are difficult to germinate are solved; and powerful technical support is provided for the development of wild tending, introducing and planting the Thesium chinense.
Owner:NANJING AGRICULTURAL UNIVERSITY

Seedling raising method for oil-used peony seeds

The invention discloses a seedling raising method for oil-used peony seeds. The method comprises the steps of (1) selecting seeds; (2) soaking the seeds for disinfecting; (3) treating the oil-used peony seeds of step (2) by using cold plasma generated by atmospheric dielectric barrier discharge for 50-60 seconds; (4) preparing a treatment solution containing 0.25mg / L of gibberellin, 2mg / L of indoleacetic acid and 3mg / L of calcium chloride; (5) performing variable temperature stratification: mixing the oil-used peony seeds treated in step (3) with fine sand, perlite and vermiculite in a ratio of 1:(3-5):(1-2):(1-2), adjusting the humidity to 40-50%, and performing variable temperature stratification in a constant temperature incubator according to three stages; (6) sowing; and (7) performing seedling management. By adopting the seedling raising method for the oil-used peony seeds, seed dormancy can be effectively broken during breeding, so that seeds are quickly germinated, germination of seeds is accelerated, the emergence rate of seeds is improved, the emergence time is shortened, the emergence is even, and the growth indexes of young seedlings such as fresh weight, plant height, root system and the like are improved to different degrees; and the method does not have pollution to the environment.
Owner:芜湖市丹洋现代农业科技发展有限公司

Winter sunlight greenhouse seedling transplantation method of potato seedling seeds

The invention discloses a winter sunlight greenhouse seedling transplantation method of potato seedling seeds. The seedling seeds collected in the same year are subjected to dormancy breaking, pregermination and the like, and cultured and transplanted in a winter. The method mainly comprises the following steps of (1) substrate preparation, (2), the dormancy breaking of the seeds, (3) the pregermination, (4) seeding, (5) seedbed management, (6) nutrition pot preparation, (7) transplantation, (8) management after the transplantation, and (9) timely harvesting. The method has the advantages that (1) the dormancy of the potato seedling seeds collected in the same year is broken effectively, so that a germination rate of the seedling seeds collected in the same year reaches above 80%; (2) the temperature and the humidity are controlled with small sheds in a sunlight greenhouse, so that chilling damages are prevented effectively; (3) enough water is sprayed before the transplantation, so that the damages to root systems of seedlings during the transplantation are reduced, and a survival rate of the transplantation of the seedlings reaches above 95%; and (4) the potato seedling seeds are collected and applied in the same year; a seed breeding period can be shortened by 1-1.5 years compared with the conventional method; and breeding, production and utilization of a new variety are accelerated.
Owner:云南省农业科学院经济作物研究所

Cultivating method of zostera aseptic seedlings

The invention relates to a cultivating method of zostera aseptic seedlings, comprising the following steps of: preserving collected zostera seeds for a month or longer at a low-temperature condition of 4 DEG C to break a seed resting state by low-temperature stimulation; then, cleaning the zostera seeds preserved at low temperature by sterilized seawater under an aseptic condition; picking strong and perfect seeds; sterilizing the seeds for 30s by using ethanol of 70 percent; sterilizing the seeds for 10min by using 10 percent sodium hypochlorite; planting the seeds into a conical bottle with an MS liquid culture medium after cleaning the seeds by the sterilized seawater; cultivating the conical bottle into an illumination cultivating box under the cultivating conditions with the temperature of 22 DEG C, the illumination intensity of 1000Lx and the illumination time of 12h; and changing the culture medium once every four days until the length of the seedlings is 2-3 cm. The cultivating method can be used for cultivating at all seasons, ensures the seedling supply in zostera scale cultivation, and is also suitable for aseptic seedling cultivation of other seedlings of zostera L.of zosteraceae of helobial.
Owner:OCEAN UNIV OF CHINA

Planting method of cherry trees

The invention discloses the technical field of fruit tree planting and provides a planting method of cherry trees. According to planting treatment, fruit pits are taken out, and airing is performed to obtain seeds; 8-12-fold damp sand are mixed with the seeds, and the mixture is refrigerated in a refrigerator under the temperature of 0 DEG C; the mixture is placed into a 1:40-1:60 pig manure compost leaching solution and a gibberellin mixing solution with the concentration of 5% to be soaked, the soaking time is 5-10 min, the seeds are taken out after soaking is finished and sown in a seedling basket, a matrix with the ratio of turfy soil to river sand being 1:1 is adopted, and germination is accelerated under the room temperature; germination acceleration is stopped after radicles extrude seed coats and the length of the seeds is 1-3 times during seed germination, the germinated seeds are taken out and the seed coats are soaked into rooting powder. On the basis of seed sand storage freezing treatment, seed dormancy is broken through, the germination rate is effectively increased through soaking in the pig manure compost leaching solution and the gibberellin mixing solution, the seed germination rate is improved to 90% or above, the number and length of seed roots are effectively increased, the problem that a root system is shallow is avoided, and the rooting rate can be further increased through the rooting powder soaking solution.
Owner:正安县高山果蔬种养殖农民专业合作社

Method for promoting seed germination of cercidiphyllum japonicum and improving germination ratio of seeds

The invention discloses a method for promoting seed germination of a cercidiphyllum japonicum and improving the germination rate of seeds. The method is mainly achieved through steps of seed selecting, seed disinfecting, laser treatment, seed dormancy breaking treatment, seed germination, seedling transplant and the like. According to the invention, laser treatment, vitriolization, hormone treatment, fluctuating temperature stratification and temperature and illumination intensity control are comprehensively utilized, the seed germination of the cercidiphyllum japonicum can be prompted from all aspects such as the selection of culture medium, the germination ratio is high, a seeding root system of the seed of the cercidiphyllum japonicum is prosperous, the growth vigor is excellent, the speed is fast, and the survival rate is high.
Owner:SUZHOU RENCHENG BIO SCI & TECH

Method for stopping Taxus chinensis var. mairei seed dormancy

The invention belongs to the technical field of plant budding, particularly relates to a method for stopping Taxus chinensis var. mairei seed dormancy, and solves the technical problem of slow budding, low budding rate and the like. The method includes the steps of A, soaking Taxus chinensis var. mairei seeds in sulfuric acid 55-65% in mass concentration for 2-4 hours, and washing the soaked seeds with flow water for 12-36 hours; B, soaking the acid etched seeds in pre-prepared solution for at least 3 hours, and soaking the soaked seeds in clean water for at least 3 hours; and C, performing low-temperature stratification or variable-temperature stratification to the seeds soaked by clean water. The method has the advantages that the method is time-saving, effective and economical, the problems that dormancy is deep, budding is difficult and budding rate is low are solved, standards are provided for seed seedling market circulation, seed quality is guaranteed, and great significance is brought to breeding cultivation development of Taxus chinensis var. mairei.
Owner:ZHEJIANG CHINESE MEDICAL UNIVERSITY

Tobacco seed germinating method

InactiveCN101611663ASolve problems in productionGood effectSeed immunisationSeed dormancyGibberellin
The invention discloses a tobacco seed germinating method, which utilizes gibberellin solution to break the seed dormancy, and promotes the germination of tobacco seeds by steps of seed soaking, cleaning, disinfecting, germinating and the like to improve the germination rate and the germinating uniformity and achieve the aims of uniform and healthy seedlings, and improvement of breeding quality.
Owner:中国烟草总公司黑龙江省公司烟草科学研究所

Germination processing method for horseradish seeds

The invention discloses a germination processing method for horseradish seeds, and the method comprises the following steps of: seed soaking-soaked seed sterilization-sterilized seed dormancy prevention treatment-seeding-seed germination. The method disclosed by the invention can effectively improve the rate of emergence of the horseradish seeds, and the statistical result shows that the rate of the emergence of the horseradish seeds can be up to more than 90%.
Owner:广元市玺府生物科技有限公司

Method for breaking sweet-scented osmanthus seed dormancy

InactiveCN103299743AReduced processing time at low temperatureShorten the timeSeed and root treatmentSeed dormancyGibberellin
The invention discloses a method for breaking sweet-scented osmanthus seed dormancy. The method comprises the following steps of seed selection, seed soaking treatment, plasma treatment, comprehensive treatment by gibberellin and hydrogen peroxide, low-temperature stratifcation treatment and the like. According to the method disclosed by the invention, proper frequency, voltage and treatment time of the plasma treatment are selected so that the later low-temperature treatment time is shortened. As the gibberellin and the hydrogen peroxide are applied to the comprehensive treatment of the seed, a substance inhibiting seed germination is effectively broken, and the time for breaking the dormancy is shortened.
Owner:苏州市阳澄湖现代农业发展有限公司

Paris polyphylla var Chinensis seed germination method

The invention discloses a paris polyphylla var Chinensis seed germination method which comprises the steps of seed peeling, gibberellin soaking, humus soil constant temperature and humidity culture and the like. According to the method, the growth characteristics of paris polyphylla var Chinensis are combined in actual production, a laminating germination accelerating method is found out for paris polyphylla var Chinensis seed propagation, a dormancy period of paris polyphylla var Chinensis seeds is broken, rapid germination of the paris polyphylla var Chinensis seeds is promoted, the germination rate is improved, the germination stage of the paris polyphylla var Chinensis is shortened from two years to 3-6 months, and the germination rate is improved from 30%-50% to 70%-90%. The problems of long seedling emergence time, low germination rate and difficult germination of the paris polyphylla var Chinensis seeds are solved, and artificial large-scale planting can be achieved.
Owner:青川县蜀道地中药材发展有限公司

Plant proteins having an abscisic acid binding site and methods of use

Proteins having binding sites for abscisic acid (ABA) and methods of use are disclosed. The physiological functions of ABA, plant life cycles, seed dormancy and ripening can be altered by manipulating the binding of ABA to its receptors.
Owner:UNIVERSITY OF MANITOBA

Breaking sleep and budding of notopterygium seeds

A dormancy waking up and germinating method for the seeds of notopterygium includes properly changing the temp to wake up said seeds in dormanant state, promoting the germination of said seeds, and controlling the temp, culture medium and sowing depth to obtain high-quality seedlings.
Owner:四川诺托璞生态药材有限公司 +2

Cereals with altered dormancy

The present invention relates to polypeptides, and polynucleotides encoding therefor, which influence seed dormancy in cereals such as wheat and barley. In particular, the present invention relates to polypeptides with ABA 8′-hydroxylase activity, and polynucleotides encoding these proteins. Also provided are cereal plants which produce seeds that have altered rates of germination, and / or levels of dormancy, when compared to seeds from wild-type plants, as well as methods of producing such plants.
Owner:COMMONWEALTH SCI & IND RES ORG

Method for releasing dormancy of tapiscia sinensis seeds and improving survival rate of growing seedlings

The invention discloses a cultivation method for releasing dormancy of tapiscia sinensis seeds and improving the survival rate of growing seedlings. The method is achieved mainly through seed selection, seed sterilization, ultrasonic treatment, hormone and varying temperature stratification treatment, seed growing seedling soil management and other steps. With utilization of growth regulator control and the varying temperature treatment and other seed dormancy breaking technologies and with use of a suitable-concentration growth regulator and an appropriate varying temperature treatment matrix, the germination time of the tapiscia sinensis seeds is greatly shortened. Moreover, by employing an appropriate temperature, illumination treatment, the appropriate growing seedling matrix and a scientific soil management mode, the survival rate of the growing seedlings is allowed to be quite high.
Owner:SUZHOU RENCHENG BIO SCI & TECH

Large-scale seedling cultivation method for Monochasma savatieri Franch. ex Maxim.

The invention belongs to the technical field of cultivation of medicinal plant seedlings, and relates to a large-scale seedling cultivation method for Monochasma savatieri Franch. ex Maxim. The large-scale seedling cultivation method for Monochasma savatieri Franch. ex Maxim. comprises the steps of seed collection, nursery land selection, plowing and fertilization, seed pretreatment, sowing and seedling management. The seed collection is conducted by using weed-proof cloth, and the weed-proof cloth surrounds a portion under Monochasma savatieri Franch. ex Maxim.; the seed pretreatment comprises the step of soaking seeds by using gibberellin. According to the large-scale seedling cultivation method for Monochasma savatieri Franch. ex Maxim., the weed-proof cloth is used for collecting the seeds, and the problem that the seeds of Monochasma savatieri Franch. ex Maxim. are difficult to collect is solved; and the gibberellin is used for pretreatment, seed dormancy is broken, and the germination rate is increased. The large-scale seedling cultivation method for Monochasma savatieri Franch. ex Maxim. is high in germination rate, easy to operate and convenient to popularize, and has a great promotion effect on protection of wild resources and development of the industry.
Owner:江西神元湖农业科技发展有限公司

Pumpkin seed germination accelerating method

The invention provides a pumpkin seed germination accelerating method comprising seed selection, seed soaking with a seed soaking agent, microwave nutrient solution seed soaking, refrigerator temperature-varying seed storage, and modified wet sand seed dressing and germination accelerating. According to the germination accelerating method, compound sodium nitrophenolate and a seed coating agent are compounded for seed soaking, the dormancy period of seeds can be shortened, a regulatory role is still played at low temperatures, cell division and germination are promoted, and pathogen infestation is resisted, so that pumpkin seedlings can grow normally under the low temperatures, the seedlings are allowed to be healthy and strong, the survival rate is high after transplanting, and the yield is increased; and with nutrient solution soaking, the quality of pumpkin is further increased.
Owner:ANHUI HUAHE SEED CO LTD

Efficient aseptic seedling raising method of monochasma sheareri

InactiveCN110495391ASolve the current situation that the natural germination rate is low and seedlings are difficult to obtainSimple technical operationPlant tissue cultureHorticulture methodsSeed dormancyInoculation methods
The invention discloses an efficient aseptic seedling raising method of monochasma sheareri. According to the efficient aseptic seedling raising method of the monochasma sheareri, through the steps ofseed treatment, surface sterilization, inoculation, transplanting and the like, the highest seed germination rate of the monochasma sheareri reaches 100%, and the transplanting survival rate reaches86%; by adopting the inoculation method, monochasma sheareri seeds are distributed in a culture medium more uniformly, cultivated aseptic seedlings are healthy and strong and grow in order, the cleanseeds can be effectively obtained, seed dormancy is broken, and the seed surface sterilization effect is ensured, so that the seeds are dispersed on the surface of the culture medium uniformly; the operation is simple and easy, the seed utilization rate can be increased, a large number of monochasma sheareri seedlings can be obtained in a short time, and wild monochasma sheareri plant resources can be effectively protected.
Owner:EXPERIMENTAL CENT OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY

Phytolacca americana seed quick-speed germination method and its application in aseptic seedlings culture

InactiveCN101451119ABreak hibernationReduced germinationPlant cellsSeed dormancyPhytolacca americana
The invention relates to the field of plant tissue culture, in particular to a method for quickly germinating phytolacca americana seeds and application of the method to aseptic seedling culture. The method comprises: soaking the phytolacca americana seeds into a concentrated sulfuric acid for a certain period, damaging the structure of phytolacca americana seed coats, breaking the sleep state of the seeds, and making the seeds be quickly subjected to imbibition and enter the germination state; simultaneously, utilizing the sterilization function of the concentrated sulfuric acid to perform sterilization treatment on the phytolacca americana seeds; subsequently, inoculating the aseptic phytolacca americana seeds on a culture medium selected by an experiment, adjusting the conditions such as temperature and illumination, and making the phytolacca americana seeds germinate quickly; and finally obtaining aseptic phytolacca americana seedlings. The method can quickly break through the sleep state of the phytolacca americana seeds, and greatly shorten the germination time and the seedling time of the phytolacca americana seeds. The invention provides a method for culturing the aseptic phytolacca americana seedlings for laboratory investigation.
Owner:NANJING NORMAL UNIVERSITY
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