47 results about "Pyrus betulaefolia" patented technology

The invention discloses a tissue-culture rapid propagation method for pyrus betulaefolia bunge. The tissue-culture rapid propagation method for the pyrus betulaefolia bunge comprises disinfecting on explants, inducing buds, propagating test tube seedlings, inducing calluses, inducing rooting and transplanting exercising seedlings. During the process of inducing rooting, the calluses are firstly induced and then the rooting is induced; obtained rooting test tube seedlings are performed seedling exercising in a triangular flask, then transferred to perlite to domesticate the exercising seedling and finally transplanted. Compared with the traditional method, the tissue-culture rapid propagation method for the pyrus betulaefolia bunge has the advantages of being high in rooting rate and survival rate of transplanting, high in propagation rate, low in cost, convenient to popularize, good in genetic stability and suitable for the pyrus betulaefolia bunge.

The invention discloses multi-lateral root fruit tree bridging technology, which is a method for performing single-head bridging on a one-year high-quality seedling of a pyrus betulaefolia rootstock which is transplanted successfully on a plurality of sides, 50 to 70 centimeters away from the ground surface, of a fruit tree trunk when a fruit tree is in a growing season. By bridging the fruit tree by the method, the rootstock and the high-quality seedling have no impedance response basically; the root is supplied with sufficient nutrition so that the fruit tree obtains increased nutrients; therefore, harvest rate and fruit quality are improved; and the disease resistance of the fruit tree is improved and a survival period is prolonged.

The invention relates to application of a pyrus betulaefolia Pb4RMYB gene and encoded protein thereof in enhancement of plant salt tolerance. The nucleotide sequence of the MYB transcription factor Pb4RMYB separated from pyrus betulaefolia is shown as SEQ ID No.1, and the encoded amino acid sequence is shown as SEQ ID NO.2. The Pb4RMYB gene is converted into arabidopsis thaliana according to an agrobacterium-mediated genetic transformation method. According to biological function verification, the obtained transgenic plant is overexpressed by the Pb4RMYB gene, and has remarkably higher tolerance to high-salinity adversity stress than wild type control plants. The pyrus betulaefolia Pb4RMYB gene has great significance in research on salt-tolerant molecules mechanism of plants, in addition,has an important function in cultivation of salt-tolerant fruit tree stocks or varieties, thereby providing high possibility for cultivation of new stress-resistant fruit tree varieties and being significant for growth of fruit trees.

The invention discloses amino acid sequences of GAI proteins of Pyrus betulaefolia and Pyrus bretschneideri, and nucleotide sequences for coding the GAI proteins, wherein the sequences are disclosed as SEQ ID NO1-4 in the sequence table. The invention also discloses a molecular identification method based on transfer of GAI mRNA between pear rootstock and scion, which comprises the following steps: (1) micrografting seedlings in test tubes by using Pyrus bretschneideri as scion and using Pyrus betulaefolia as rootstock; (2) looking for the respective specific enzyme-digestion sites of the GAI genes of the Pyrus bretschneideri and the Pyrus betulaefolia, and respectively designing a primer at the upstream and downstream of the enzyme-digestion site; (3) and carrying out enzyme-digestion on the Pyrus betulaefolia, the Pyrus bretschneideri, and the RT-PCR product of the Pyrus betulaefolia and the Pyrus bretschneideri after grafting, and comparing the enzyme-digestion spectra before and after the grafting to identify the transfer conditions. The method is quick, sensitive, accurate, simple and convenient, and is applicable to other pear plants.

The invention provides an anti-abiotic-stress transcription factor PbrbHLH92 and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the anti-abiotic-stress transcription factor PbrbHLH92 is as shown in SEQ ID No. 1. The transcription factor PbrbHLH92 capable of increasing plant abiotic stress resistance is screened from Pyrus betulaefolia, and protein encoded by the transcription factor PbrbHLH92 belongs to nucleoprotein. Experiments show that the anti-abiotic-stress transcription factor PbrbHLH92 plays an important role in plant drought and salt stress resistance, can increase plant sensitivity to abscisic acid and plays an important role in various abiotic stress resisting. The anti-abiotic-stress transcription factor PbrbHLH92 is transferred into tobacco or Pyrus betulaefolia, the active oxygen eliminating ability of the obtained transgenic tobacco or Pyrus betulaefolia can be effectively enhanced as compared with that of wild tobacco or Pyrus betulaefolia, and the drought resistance of the transgenic tobacco is increased effectively.

The invention relates to a method for promoting germination through release of pyrus betulaefolia seed dormancy by sodium malonate and belongs to the technical field of plant production. The method for promoting the germination of pyrus betulaefolia seeds comprises the following main steps: 1) collection and cleaning of seeds; 2) seed soaking in clean water and seed disinfection; 3) treatment by a chemical agent and seed cleaning; and 4) sowing. The pyrus betulaefolia seed treated by the sodium malonate is applied, begins to germinate in three days after the sowing and can totally germinate in one week around; and the germination rate is high and can reach more than 85 percent. The method initially finds that the pyrus betulaefolia seed can release the seed dormancy through the treatment of the sodium malonate without low-temperature lamination treatment. The application of the research can effectively shorten the germinating time and cross-breeding cycle of pyrus betulaefolia and simplify the embryo culturing procedure.

The invention relates to a seed growing method for pyrus betulaefolia. The seed growing method comprises the steps of seed collection, soaking, multi-medium lamination treatment, performing sprouting cultivation through a manmade climate box and the like. By the adoption of pyrus betulaefolia seeds treated by the seed growing method disclosed by the invention, the sprouting rate is increased, and the seed treatment expense is lowered; meanwhile, the shortcomings of low seed sprouting speed and untidy seedling emerge are overcome, so that tree seeds with difficulty in seedling growing are easy to multiply and are multiplied quickly; the seed growing method is easy to popularize in production, and the sprouting rate is over 95 percent.

The invention provides a method for rapidly removing pyrus betulaefolia seeds from dormancy to promote sprouting thereof, and relates to a method for removing seed dormancy and a seed sprouting technology, belonging to the technical field of plant production. The invention mainly draws on a characteristic that a chemical agent 2-thiopseudourea can break up seed embryo dormancy to remove pear seeds from seed embryo dormancy so as to promote the pear seeds to germinate, improve sprouting rate and tidy the sprouting. The sprouting method by using the 2-thiopseudourea comprises the following three steps: (1) peeling seedcases of ripe fruits, washing by hands and removing pulps, using NaClO with the mass volume ratio of 20% to soak and treat the seeds to remove the pulps adhered on the seedcases; (2) soaking for 8 min by using a mercury bichoride solution with the mass volume ratio of 0.1% for sterilization, taking the clean pyrus betulaefolia seeds to be respectively placed in a 2-thiopseudourea solution with the mass volume ratio of 0.8% to soak for 24h at room temperature; and (3) washing the soaked pyrus betulaefolia seeds for five-six times by fresh water, and then seeding.

The invention belongs to the technical fields of cytobiology and biology, and discloses a preparation method of pyrus betulaefolia protoplast. According to the method, through the pretreatment of a pyrus betulaefolia material and separation treatment of cytoplasm and cytoderm, the mesophyll tissue of pyrus betulaefolia is subjected to enzymolysis through the combination of cellulase, macerase and pectinase, the preparation of pyrus betulaefolia protoplast is effectively realized, the problem that the pyrus betulaefolia protoplast is difficult to separate is overcome, and the preparation method of the pyrus betulaefolia protoplast is effectively created. The method breaks through the bottleneck of genetic transformation of pyrus betulaefolia, establishes a pyrus betulaefolia cell engineering technical system, and provides a technical base for the genetic transformation of the pyrus betulaefolia protoplast, the transient expression examination of gene and the fusion of the protoplast.

The invention discloses a method for quickly relieving dormancy of pyrus betulaefolia seeds, relates to hormone treatment and germination acceleration, and belongs to the field of plant physiology and production cultivation. According to the method, by removing peels of the seeds, covering the seeds with degreasing cotton, treating the peeled off seeds with a gibberellin and glucose mixed solution and using proper exogenous hormone and the moisture retention property of the degreasing cotton, the dormancy of the seeds is broken through. The method has the advantages of simple steps, low loss, relatively short time and the like; the effect of relieving the dormancy is good, the number of mildewed seeds is relatively small, and germinated seeds can be used for sowing or subsequent experiment.

The invention provides a method for in-vitro micro-grafting of tissue culture seedlings of Danshui pyrus pyrifolia, the method includes the steps of inoculating inducing medium with stem sections withbuds, serving as explants, to form sterile test tube plantlets through cultivation; transplanting the sterile test tube plantlets to proliferation medium to obtain rootless tissue culture seedlings through proliferation, and taking the proliferous rootless tissue culture seedlings as micro-grafting scions; inoculating seed germination medium with pyrus betulaefolia seeds to form stocks through cultivation; cutting the scions into wedged bases, putting the wedged bases into notches of the stocks to obtain micro-grafted seedlings; transplanting the micro-grafted seedlings into cultivation medium after domestication, and cultivating the seedlings in a plastic arched shed after transplanting, the method has the advantages that tissue culture and grafting techniques are combined, and the proliferous tissue culture seedlings and pyrus betulaefolia seedlings are subjected to micro-grafting, so that the induction process for rooting of the tissue culture seedlings is omitted, seedling cultureprocedures are simplified, seedling culture time is shortened, and resilience and high yield of nursery stocks are improved.

The invention provides a root medium for pyrus betulaefolia tissue culture seedlings. The root medium comprises an inorganic salt halved MS medium, 0.25 to 0.5 mg / L of 3-indolebutyric acid, 6 to 9 g / L of agar, 0.3 to 0.5 g / L of active carbon and 15 to 30 g / L of saccharose. The invention also provides a rooting method for the pyrus betulaefolia tissue culture seedlings. The rooting method comprises the following steps: selecting well-grown pyrus betulaefolia tissue culture seedlings, cutting into stems with the lengths of 1 to 2.5 cm, and putting into the root medium for rooting culture to obtain the pyrus betulaefolia tissue culture seedlings. In combination with explant material drawing manner and matching of exogenous auxin, the efficient and simple root medium for the pyrus betulaefolia tissue culture seedlings is explored, and compared with relatively conventional methods, the root medium is high in rooting percentage and high in survival rate of transplanting, simple and convenient in operation and low in cost, and is applicable to pyrus betulaefolia tissue culture rapid propagation.

The invention discloses a making method of pyrus betulaefolia bunge fruit and rubus taiwanianus tea cream. The pyrus betulaefolia bunge fruit and rubus taiwanianus tea cream is mainly prepared from the following raw materials of pyrus betulaefolia bunge fruits and rubus taiwanianus, and is prepared through the steps of pretreating and preparing the pyrus betulaefolia bunge fruits, treating the rubus taiwanianus, preparing tea powder decoction liquid, performing mixing, performing crushing, preparing the tea cream, performing sterilizing, performing packaging and the like. According to the tea cream prepared by the method disclosed by the invention, the pyrus betulaefolia bunge fruits and the rubus taiwanianus which are green, healthy, rich in nutrition, and broad in sources are used as raw materials, so that not only is the production cost reduced, but also the new species of the tea cream and the nutrient components of the tea cream are enriched; pyrus betulaefolia bunge fruit residues are treated with multizymes, so that the utilization efficiency of the pyrus betulaefolia bunge fruits is further improved; sublimation drying is performed, so that the condition that high temperature enables nutrient substances in the raw materials to be destroyed is avoided, and active components of the raw materials are fully reserved; finished products of the pyrus betulaefolia bunge fruit and rubus taiwanianus tea cream are convenient to carry, bright in color, and pure in mouth feel, and have the effects of loosening bowel to relieve constipation, reducing swelling, easing a pain, removing food stagnancy, being helpful for digestion, invigorating the spleen, regulating vital energy and the like.

The invention belongs to the technical field of propagation of pears and particularly discloses an adventitious-bud-grafted rapid propagation method for pears. According to the method, annual or biennial Pyrus betulaefolia bunge or Pyrus calleryana solid-wood seedlings are selected as rootstocks, adventitious buds obtained through tissue culture and subculture propagation of Pyrus sinensis or goldpears are selected as scions, the rootstocks and the scions are pretreated before grafting, then, grafting is carried out in a greenhouse, T-shaped bud grafting is adopted as a grafting method, basalportions of adventitious-bud scion stems are beveled, then, the beveled basal portions of the adventitious-bud stems are dipped with a hormone mixed solution, the dipped adventitious-bud stems are inserted into T-shaped cuts of rootstock stems, grafted ports are subjected to sealing and plastic bag covering treatment, and then, grafted post-management is carried out. According to the adventitious-bud-grafted rapid propagation method for the pears, provided by the invention, culture is carried out in the greenhouse, the operation is simple, the culture period is short, the survival rate is high, the problem that adventitious roots of the pears are difficult in regeneration can be effectively solved, and a technical support is provided for tissue culture regeneration, industrialized seedling rearing and functional genomics research of the pears.

The invention belongs to the technical field of plywood machining, and particularly relates to a method for manufacturing a plywood by using pyrus betulaefolia saw dust. The method comprises the following five steps of saw dust pretreatment, saw dust surface treatment, adhesive preparation, molding and veneer pasting, and finally, the pyrus betulaefolia saw dust is successfully manufactured into the plywood. According to the method, the pyrus betulaefolia saw dust is recycled, the resource utilization rate is high, the waste is reduced, the effects of being energy-saving and environment-friendly are achieved, moreover, the manufactured plywood is high in strength, strong in insect-pest-resistant capability and long in service life.

The invention belongs to the technical field of fruit tree breeding, and particularly relates to an efficient pear tree rot disease resistance breeding method. The method comprises the steps of performing hybridized combination selection, hybridization pollination, hybrid seed lamination, seeding and transplanting and pear tree rot disease bacterium inoculation; weeding out disclosed plants according to the filial generation plant number in a hybridized combination; for the hybridized combination with the single plant number being 500 or above, weeding out the single plants with the diseased rate being 40 percent or higher and the disease spot length being 2cm or above; for the hybridized combination with the single plant number being smaller than 500, weeding out the single plants with the diseased rate being 70 percent or higher and the disease spot length being 2cm or above; then, selecting the fullest buds in a 10cm range at the topmost end of the resistance plant to be grafted onto the pyrus betulaefolia stocks.

The invention relates to a pyrus betulaefolia cold-resistant factor PbrMYB5 and the application thereof and belongs to the technical field of plant genetic engineering. The amino acid sequence of thecold-resistant factor PbrMYB5 provided by the invention is of SEQ ID NO.1 as shown in the specification. By adopting the cold-resistant factor provided by the invention, the cold resistance of a plantcan be remarkably improved, novel germplasm resources can be provided for plant molecular breeding, novel genetic resources can be provided for green agriculture and modern agriculture, the agricultural production risks can be reduced, and sustainable development of agriculture can be achieved.

The invention discloses a pyrus betulaefolia salt-tolerant gene, a protein, a recombinant vector and application, and relates to the technical field of gene engineering. The sequence of the salt-tolerant gene PbbHLH67 is as shown in SEQ ID NO.1. The gene is used for constructing an overexpression vector and a silent vector, stable inheritance of the gene in arabidopsis thaliana and instantaneous transformation expression of the gene in pyrus betulaefolia are achieved through agrobacterium tumefaciens-mediated genetic transformation, and salt-tolerant function verification is carried out on obtained positive plant seedlings. Results show that the PbbHLH67 gene can effectively increase the salt tolerance of plants. It is found that a novel gene resource is provided for plant stress resistance molecular breeding, a reference is provided for plant cultivation of saline-alkali soil in China and even in the world, and the utilization rate of saline-alkali soil in China is improved.

The invention discloses a close planting cultivation technology of red pear dwarf interstocks. Zaosu red pear and Wuhong pear are selected as red pear varieties, Zhongai No.5 is selected as the dwarfinterstocks, pyrus calleryana or pyrus betulaefolia is selected as grafting stock, pyrus calleryana or pyrus betulaefolia seeds are sown in furrows in spring of the first year, the dwarf interstock Zhongai No.5 is grafted on the grafting stock in early spring of the second year, red pears are grafted and cultivated on the dwarf interstocks in early spring of the third year, and the interstocks are27-30 cm long; nursery stocks are transplanted in winter of the third year. The dwarf interstock Zhongai No.5 can weaken growth vigor of branches and promote branching and formation of flower buds, crowns are reduced, and ventilation and light transmission are good; diseases are reduced, disease and pest control is facilitated, and coloring of fruits is facilitated, so that the high yield effectcan be realized.

The invention discloses a mecodium badium and rutin compound nursing medicament for treating skin injury. The medicament mainly comprises traditional Chinese medicine components and Western medicine components, wherein the traditional Chinese medicine components are prepared from mecodium badium as a main material and the following traditional Chinese medicines as auxiliary materials through extraction: wood of true lacquertree, coriolus hirsutus, root of actinidia, root of phyllodium pulchellum, piper yunnanense Tseng, eritrichium pectinatum, cipedessa cinerascens, sabina chinensis, lagochilus lanatonodus, caesalpinia decapetala, omei mountain bambusa, euonymus hamiltonianus, colebrookea oppositi folia Smith, pyrus betulaefolia (bark), buddleja asiatica, ajuga forrestii Diels and oxystelma esculentum; the Western medicine components comprise rutin, silk polypeptide, glycosaminoglycan, tocopherol, fibronectin, lignin, L-proline and histidine. The nursing medicament has the effects of removing toxicity, clearing heat, promoting granulation, stopping bleeding, promoting qi to activate blood, dehumidifying, diminishing swelling, and preventing tumor, has good effects of preventing oxidation and removing free radicals, promotes regeneration of tissue cells, and enhances the immunity of the human body; the treatment from inside to outside is realized, so that the defect that the conventional external use medicament cures the symptoms, but cannot cure the diseases is overcome.

Provided is a grafting method for cultivating a single-branch strong seedling through a pyrus betulaefolia large stock. In a planned pear garden, the pyrus betulaefolia large stock is selected, after soil unfreezing, planting is carried out, then grafting during a stock germination stage is carried out, and after grafting, a grafting body is effectively protected. After a seedling survives, field matching management measures are enhanced, and the single-branch strong seedling can be cultivated in autumn. The grafting method has a key effect on cultivating of a pear garden long, thin and narrow crown tree shape in a labor-saving, efficient and modern cultivating mode and early-stage fruiting and high yield.

The invention discloses a planting method of pyrus betulaefolia and belongs to the field of agricultural planting. The method includes the following steps of selecting sandy soil or light soil which is smooth in irrigation and drainage function, fertile, low in salt and alkali content and unlikely to be damaged by diseases and pests, exposing the soil under the blazing sun for 3-5 days after ploughing, then smashing soil blocks, fully applying base fertilizer and forming ridges, wherein the ridge width is 60-80 cm, the depth of each ridge gutter is 20-35 cm, and the width of each ridge gutteris 30-35 cm; soaking seeds in water for 10-15 min, taking out the seeds, mixing the seeds with plant ash and dry sand uniformly, laying a layer of sand on the surfaces of the ridges, uniformly scattering the seeds on the surfaces of the ridges, covering the seeds with a river sand layer in the thickness of 0.3-0.5 cm, covering the river sand with straw, then watering the straw, and covering the straw with thin film; conducting seedling bed management; carrying out transplanting. By means of the planting method of pyrus betulaefolia, the germination time of pyrus betulaefolia can be shortened,and the survival rate of seedlings is increased.

The invention relates to the technical field of seedling cultivation, in particular to a seedling raising method suitable for root cutting rapid propagation of pyrus betulaefolia, which comprises the following steps: (1) nursery selection; (2) nursery land preparation; (3) seminal root selection; (4) cutting preparation; (5) cutting seedling raising; (6) cultivation management; and (7) seedling outplanting. Through effective utilization of the method, the progeny separation phenomenon occurring in the propagation of pyrus betulaefolia seeds is avoided, the excellent characters and inherent characteristics of original parent varieties of pyrus betulaefolia are maintained, the propagation period of the seedlings is shortened, the propagation cost of the seedlings is reduced, the propagation efficiency of the pyrus betulaefolia seedlings is improved, the effect of the good pyrus betulaefolia varieties can be played to the maximum extent, and healthy development of the fruit tree industry is promoted. The method and the thought of the improved pyrus betulaefolia variety propagation are innovated and optimized, a new way for improved pyrus betulaefolia variety propagation is opened up, and the method is an indispensable technical measure in the field of improved pyrus betulaefolia variety propagation.

The invention discloses a pesticide for mulberry planting and provides a preparation method of the pesticide. The pesticide is prepared from the following raw materials in parts by weight: 25-35 parts of kochia scoparia, 25-35 parts of pyrus betulaefolia, 20-25 parts of tamarix chinensis, 15-18 parts of stone-like omphalia, 15-18 parts of spina gleditsiae, 8-12 parts of golden larch bark, 8-12 parts of fructus cnidii, 4-6 parts of silkworm excrement, 1-3 parts of concha arcae and 1-3 parts of bezoar. The pesticide for the mulberry planting and the preparation method of the pesticide have the benefits that insects in a mulberry planting process can be effectively killed; via comparison validation, an insect damage rate is decreased by 20% as compared with that of a mulberry without application of the pesticide; and the pesticide decreases the insect damage rate by approximately 10% as compared with marketed similar products; and the pesticide has good market prospects.

A novel nano biological material-nano chitin aqueous suspension prepared through acidolysis and deacylation is mixed with a fertilizer to promote growth of pyrus betulaefolia seedlings and improve theabsorption and utilization rate of the fertilizer. The nano water suspending agent is a nano chitin crystal particle with a polycation property, and data display thata various growth indexes of the pyrus betulaefolia seedlings can almost reach the same effect as that of the 100% conventional fertilization amount under the condition of applying 75% fertilizer and nano chitin; with applying 50% fertilizer and the nano chitin, the nutritional growth indexes such as the plant height, the stem diameter, the fresh / dry weight and the like of the pyrus betulaefolia seedlings can be significantly improved, so that the absorption amount of the seedlings on nitrogen elements is significantly increased (the influence on phosphorus and potassium elements is small). The results preliminarily show thatthe nano chitin which is a natural polymer material has a good synergistic effect when being mixed with the fertilizer for application, and the utilization rate of the fertilizer can be increased by 25%-50%.

The invention belongs to the technical field of fruit tree breeding, and particularly relates to an efficient pear tree rot disease resistance breeding method. The method comprises the steps of performing hybridized combination selection, hybridization pollination, hybrid seed lamination, seeding and transplanting and pear tree rot disease bacterium inoculation; weeding out disclosed plants according to the filial generation plant number in a hybridized combination; for the hybridized combination with the single plant number being 500 or above, weeding out the single plants with the diseased rate being 40 percent or higher and the disease spot length being 2cm or above; for the hybridized combination with the single plant number being smaller than 500, weeding out the single plants with the diseased rate being 70 percent or higher and the disease spot length being 2cm or above; then, selecting the fullest buds in a 10cm range at the topmost end of the resistance plant to be grafted onto the pyrus betulaefolia stocks.

The invention discloses amino acid sequences of GAI proteins of Pyrus betulaefolia and Pyrus bretschneideri, and nucleotide sequences for coding the GAI proteins, wherein the sequences are disclosed as SEQ ID NO1-4 in the sequence table. The invention also discloses a molecular identification method based on transfer of GAI mRNA between pear rootstock and scion, which comprises the following steps: (1) micrografting seedlings in test tubes by using Pyrus bretschneideri as scion and using Pyrus betulaefolia as rootstock; (2) looking for the respective specific enzyme-digestion sites of the GAI genes of the Pyrus bretschneideri and the Pyrus betulaefolia, and respectively designing a primer at the upstream and downstream of the enzyme-digestion site; (3) and carrying out enzyme-digestion on the Pyrus betulaefolia, the Pyrus bretschneideri, and the RT-PCR product of the Pyrus betulaefolia and the Pyrus bretschneideri after grafting, and comparing the enzyme-digestion spectra before and after the grafting to identify the transfer conditions. The method is quick, sensitive, accurate, simple and convenient, and is applicable to other pear plants.