Method for in-vitro micro-grafting of tissue culture seedlings of Danshui pyrus pyrifolia

Abstract

The invention provides a method for in-vitro micro-grafting of tissue culture seedlings of Danshui pyrus pyrifolia, the method includes the steps of inoculating inducing medium with stem sections withbuds, serving as explants, to form sterile test tube plantlets through cultivation; transplanting the sterile test tube plantlets to proliferation medium to obtain rootless tissue culture seedlings through proliferation, and taking the proliferous rootless tissue culture seedlings as micro-grafting scions; inoculating seed germination medium with pyrus betulaefolia seeds to form stocks through cultivation; cutting the scions into wedged bases, putting the wedged bases into notches of the stocks to obtain micro-grafted seedlings; transplanting the micro-grafted seedlings into cultivation medium after domestication, and cultivating the seedlings in a plastic arched shed after transplanting, the method has the advantages that tissue culture and grafting techniques are combined, and the proliferous tissue culture seedlings and pyrus betulaefolia seedlings are subjected to micro-grafting, so that the induction process for rooting of the tissue culture seedlings is omitted, seedling cultureprocedures are simplified, seedling culture time is shortened, and resilience and high yield of nursery stocks are improved.

Description

technical field [0001] The invention relates to the technical field of sand pear seedling cultivation and propagation, in particular to a method for in vitro micro-grafting of freshwater sand pear tissue culture seedlings. Background technique [0002] Danshui sand pear is a famous sand pear named after the place of origin. It is mainly produced in Danshui, Huiyang District, Huizhou City, Guangdong Province, which is the hometown of General Ye Ting. It is deeply favored by people and once enjoyed a good reputation in South of the Five Ridges. With the development of manufacturing and processing industries in Huiyang District after the reform and opening up and the popularization and planting of local litchi, longan and other fruit trees, the cultivation area of ​​freshwater pears has been greatly reduced, and the industry of freshwater pears has gradually declined. At present, there is a saying that the freshwater sand pear industry "does not exist" (Zhao Fei, Ni Genjin, Zh...

AI Technical Summary

Problems solved by technology

Although the freshwater pear seedlings rapidly propagated by tissue culture have the excellent characteristics of the freshwater pear mother plant, and have the same set of genetic information as the excellent mother plant, the tissue culture seedlings formed directly from the test tube seedlings do not have the same characteristics as the Tangli pear. In other words, pear seedlings do not have root systems adapted to the local soil environment, and do not have the stress resistance and high-yield foundation of traditional grafted seedlings.

In addition, sand pear is a woody plant that is difficult to root, and tissue culture is used to directly promote the rooting of test-tube seedlings. Due to the influence of the physiological state of test-tube seedlings, the rooting rate is not high and unstable.