Method for in-vitro micro-grafting of tissue culture seedlings of Danshui pyrus pyrifolia

A technology of tissue culture seedlings and micro-grafting, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of grafted seedlings without stress resistance and high yield foundation, pear seedlings do not have, and rooting rate is not high, and achieve Easy to produce wound healing, avoid infection, and grow rapidly

Pending Publication Date: 2018-11-13
HUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the freshwater pear seedlings rapidly propagated by tissue culture have the excellent characteristics of the freshwater pear mother plant, and have the same set of genetic information as the excellent mother plant, the tissue culture seedlings formed directly from the test tube seedlings do not have the same characteristics as the Tangli pear. In other words, pear seedlings do not have root sy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The method for micro-grafting in the test tube of the freshwater sand pear tissue culture seedling provided by the present embodiment comprises:

[0034] Explant pretreatment steps: Collect freshwater sand pear shoots with a length of 5-10cm in the wild, immediately remove the young leaves, put them in a sealed bag, put them in an ice box, and take them back, rinse with tap water to clean dirt, dust, and mud Sand and other surface debris should be soaked in saturated detergent solution for 5 minutes, then rinse the detergent water with tap water and change the water 3 times, then rinse with running tap water for 5 hours, and finally seal it with a sealed bag and place it at 4 °C refrigerator for later use.

[0035] Induction culture steps: put the explants of freshwater pear explants in 0.1% mercuric chloride solution for disinfection for 16 minutes, then rinse them with sterile water for 5 times, dry them with sterile filter paper, cut them into terminal buds and Stem...

Embodiment 2

[0049] The method for micro-grafting in the test tube of the freshwater sand pear tissue culture seedling provided by the present embodiment comprises:

[0050] Explant pretreatment steps: Collect freshwater sand pear shoots with a length of 5-10cm in the wild, immediately remove the young leaves, put them in a sealed bag, put them in an ice box, and take them back, rinse with tap water to clean dirt, dust, and mud Sand and other surface debris should be soaked in a saturated laundry detergent solution for 7.5 minutes, then rinsed with tap water and changed the water 4 times, then rinsed with running tap water for 8.5 hours, and finally sealed with a sealed bag and placed in 7 °C refrigerator for later use.

[0051] Induction culture steps: put the explants of freshwater pear explants in 0.2% mercuric chloride solution for disinfection for 13 minutes, then rinse them with sterile water for 5 times, dry them with sterile filter paper, cut them into terminal buds and Lateral bu...

Embodiment 3

[0065] The method for micro-grafting in the test tube of the freshwater sand pear tissue culture seedling provided by the present embodiment comprises:

[0066] Explant pretreatment steps: Collect freshwater sand pear shoots with a length of 5-10cm in the wild, immediately remove the young leaves, put them in a sealed bag, put them in an ice box, and take them back, rinse with tap water to clean dirt, dust, and mud Sand and other surface debris should be soaked in a saturated laundry detergent solution for 10 minutes, then rinsed with tap water and changed the water 6 times, then rinsed with running tap water for 12 hours, and finally sealed with a sealed bag and placed in 10 °C refrigerator for later use.

[0067] Induction culture steps: put the explants of freshwater Pear explants in 0.3% mercuric chloride solution for disinfection for 10 minutes, then rinse them with sterile water for 6 times, dry them with sterile filter paper, cut them into terminal buds and Stem segmen...

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Abstract

The invention provides a method for in-vitro micro-grafting of tissue culture seedlings of Danshui pyrus pyrifolia, the method includes the steps of inoculating inducing medium with stem sections withbuds, serving as explants, to form sterile test tube plantlets through cultivation; transplanting the sterile test tube plantlets to proliferation medium to obtain rootless tissue culture seedlings through proliferation, and taking the proliferous rootless tissue culture seedlings as micro-grafting scions; inoculating seed germination medium with pyrus betulaefolia seeds to form stocks through cultivation; cutting the scions into wedged bases, putting the wedged bases into notches of the stocks to obtain micro-grafted seedlings; transplanting the micro-grafted seedlings into cultivation medium after domestication, and cultivating the seedlings in a plastic arched shed after transplanting, the method has the advantages that tissue culture and grafting techniques are combined, and the proliferous tissue culture seedlings and pyrus betulaefolia seedlings are subjected to micro-grafting, so that the induction process for rooting of the tissue culture seedlings is omitted, seedling cultureprocedures are simplified, seedling culture time is shortened, and resilience and high yield of nursery stocks are improved.

Description

technical field [0001] The invention relates to the technical field of sand pear seedling cultivation and propagation, in particular to a method for in vitro micro-grafting of freshwater sand pear tissue culture seedlings. Background technique [0002] Danshui sand pear is a famous sand pear named after the place of origin. It is mainly produced in Danshui, Huiyang District, Huizhou City, Guangdong Province, which is the hometown of General Ye Ting. It is deeply favored by people and once enjoyed a good reputation in South of the Five Ridges. With the development of manufacturing and processing industries in Huiyang District after the reform and opening up and the popularization and planting of local litchi, longan and other fruit trees, the cultivation area of ​​freshwater pears has been greatly reduced, and the industry of freshwater pears has gradually declined. At present, there is a saying that the freshwater sand pear industry "does not exist" (Zhao Fei, Ni Genjin, Zh...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 曾令达彭长连宋冠华尹艳郑倩翟秋艳
Owner HUIZHOU UNIV
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