5 results about "Pyrus serotina" patented technology

This invention discloses a method for establishing a high-efficiency regeneration system for *Pyrus pyrifolia* leaves, belonging to the field of plant tissue culture technology. The method includes: spreading a layer of sorbitol solution on the surface of a culture medium; inoculating *Pyrus pyrifolia* seedlings into the medium and placing them in a dark environment for etiolation treatment; taking etiolated leaves from the *Pyrus pyrifolia* seedlings and inoculating them in a regeneration medium for dark culture, then transferring them to light for further culture to produce adventitious buds; after the adventitious buds reach the target length, cutting off their lateral buds and placing them on a subculture medium to obtain regenerated seedlings. This invention, by adding sorbitol solution before dark treatment, can significantly shorten the time to obtain etiolated material to 7 days, and the etiolated plants are in better condition after three weeks; furthermore, by placing *Pyrus pyrifolia* tissue culture seedlings in a dark environment for dark culture, the physiological state of the tissue culture seedlings is altered, and the obtained etiolated leaves are inoculated into the regeneration medium, improving the regeneration efficiency, and the material acquisition is not affected by the season.

The application discloses a pear anthracnose resistance gene PpbHLH030 and application thereof, and belongs to the field of plant genetic engineering. PpbHLH030 The nucleotide sequence of the gene is shown as SEQ ID NO:1, the protein sequence encoded by the gene is shown as SEQ ID NO:2, the gene is significantly up-regulated in expression under the induction of Guignardia bidwelli and MeJA, the gene is located in a plant cell nucleus and has a conserved bHLH domain. PpbHLH030 The construction of an overexpression vector and the transformation of 'eggplant pear' calluses increase the anthracnose resistance of the calluses, and the silencing of the pear fruit PpbHLH030 gene increases the anthracnose susceptibility. The application provides an important gene resource for the cultivation of pear anthracnose resistance varieties.

This invention discloses a pear resistance to ring rot disease-related protein, its encoding gene, and its applications. The amino acid sequence of the pear resistance to ring rot disease-related protein is shown in SEQ ID NO:1. The gene encoding the pear resistance to ring rot disease-related protein, PbrTLP15, has a nucleotide sequence shown in SEQ ID NO:2. Overexpression of PbrTLP15 enhances the plant's resistance to *B. dothidea*. Virus-induced gene silencing (VIGS) revealed that silencing PbrTLP15 reduces the resistance of pear plants to *B. dothidea*. These results all indicate that PbrTLP15 possesses anti-*B. dothidea* activity. This invention provides a theoretical basis for studying the role of pear PbrTLP15 in resistance to ring rot and exploring the molecular mechanism of pear disease resistance, and also provides new insights and resources for breeding pear varieties resistant to ring rot.

This invention provides a method for semi-restricted root cultivation of pears in southern China, belonging to the field of fruit tree seedling technology. Addressing the problems of uncontrolled root growth, susceptibility to root rot, and prolonged juvenile stage associated with traditional seedling cultivation in the rainy spring and hot, humid summer conditions of southern China, this invention utilizes a multi-span greenhouse, using non-woven fabric seedling bags as root restrictors, filled with a cultivation substrate of peat moss, vermiculite, and perlite. 40-50 day old *Pyrus pyrifolia* or *Pyrus pyrifolia* seedlings are transplanted. Water and fertilizer are precisely controlled through a drip irrigation system, maintaining substrate humidity at 70%-80% after transplanting. Summer drip irrigation is used for supplemental watering, and the following spring, shoots are promoted. A suitable drought treatment is applied from mid-May to June to shorten the juvenile stage. Combined with temperature and humidity regulation and central leader shaping pruning, the seedlings are "shapely from the nursery stage." The pear seedlings cultivated using this invention for two years have an average height of 170cm, and the incidence of root rot is only 2.20%, a reduction of 89.7% compared to traditional cultivation, achieving standardized and intensive seedling production.