9 results about "Pyrus serotina" patented technology

The invention discloses an application of a PpWRKY53 transcription factor in improving heat resistance of Chinese pear fruits, the transcription factor is a positive regulatory factor of Chinese pears responding to high temperature stress, overexpression of the transcription factor PpWRKY53 gene in the Chinese pear fruits can significantly reduce hydrogen peroxide accumulation and membrane lipid peroxidation caused by high temperature, effectively alleviate water stain type browning symptoms of the fruits, and improve the heat resistance of the Chinese pears. The fruit hardness is improved. The invention provides a key gene resource for creating new germplasm of heat-resistant Chinese pears and breeding new varieties with high-temperature-resistant characteristics, and has important scientific and application values for ensuring stable production of summer Chinese pear industry and improving fruit quality and economic benefits.

The invention discloses an application of a single nucleotide polymorphism site as a detection target in preparation of a kit for detecting the early flowering stage character of pears, and belongs to the technical field of plant molecular markers, the single nucleotide polymorphism site is that the 247th site of a nucleotide sequence shown as SEQ ID NO.1 has polymorphism of C > T, and the 247th site of the nucleotide sequence shown as SEQ ID NO.2 has polymorphism of C > T; the single nucleotide polymorphism site is used as a detection target spot for detecting the early flowering stage character of the pear; the method is rapid, simple and convenient, has high specificity, can realize high-throughput detection, and has important significance in early flowering stage variety identification, new variety breeding, breeding period shortening and breeding efficiency improvement.

The invention discloses an accurate monitoring and efficient prevention and treatment system for black spot of dwarf and close-planting bergamot pear trees, which belongs to the technical field of prevention and treatment of black spot of bergamot pear trees and comprises a prevention system and a treatment system for black spot of bergamot pear trees. According to the precise monitoring and efficient prevention and treatment system for the dwarf densely-planted fragrant pear tree black spot, precise monitoring and timely and targeted pesticide spraying of the dwarf densely-planted fragrant pear tree black spot can be achieved, so that the efficient prevention and treatment effect is achieved, the pesticide use amount can be greatly reduced, the production cost of fruit farmers is reduced, and the economic benefits of the fruit farmers are improved; the produced bergamot pears are small in pesticide residue, the quality of fruits which are not invaded by diseases is better, and the commodity value is higher; the damage of pesticide control to the ecological environment can be reduced, and the living conditions of surrounding residents are improved.

1. Name of the designed product: storage cylinder (duck pear). 2. Use of the designed product: storage cylinder for storing articles. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: perspective view.

This invention discloses a method for establishing a high-efficiency regeneration system for *Pyrus pyrifolia* leaves, belonging to the field of plant tissue culture technology. The method includes: spreading a layer of sorbitol solution on the surface of a culture medium; inoculating *Pyrus pyrifolia* seedlings into the medium and placing them in a dark environment for etiolation treatment; taking etiolated leaves from the *Pyrus pyrifolia* seedlings and inoculating them in a regeneration medium for dark culture, then transferring them to light for further culture to produce adventitious buds; after the adventitious buds reach the target length, cutting off their lateral buds and placing them on a subculture medium to obtain regenerated seedlings. This invention, by adding sorbitol solution before dark treatment, can significantly shorten the time to obtain etiolated material to 7 days, and the etiolated plants are in better condition after three weeks; furthermore, by placing *Pyrus pyrifolia* tissue culture seedlings in a dark environment for dark culture, the physiological state of the tissue culture seedlings is altered, and the obtained etiolated leaves are inoculated into the regeneration medium, improving the regeneration efficiency, and the material acquisition is not affected by the season.

This invention discloses a pear resistance to ring rot disease-related protein, its encoding gene, and its applications. The amino acid sequence of the pear resistance to ring rot disease-related protein is shown in SEQ ID NO:1. The gene encoding the pear resistance to ring rot disease-related protein, PbrTLP15, has a nucleotide sequence shown in SEQ ID NO:2. Overexpression of PbrTLP15 enhances the plant's resistance to *B. dothidea*. Virus-induced gene silencing (VIGS) revealed that silencing PbrTLP15 reduces the resistance of pear plants to *B. dothidea*. These results all indicate that PbrTLP15 possesses anti-*B. dothidea* activity. This invention provides a theoretical basis for studying the role of pear PbrTLP15 in resistance to ring rot and exploring the molecular mechanism of pear disease resistance, and also provides new insights and resources for breeding pear varieties resistant to ring rot.

This invention provides a method for semi-restricted root cultivation of pears in southern China, belonging to the field of fruit tree seedling technology. Addressing the problems of uncontrolled root growth, susceptibility to root rot, and prolonged juvenile stage associated with traditional seedling cultivation in the rainy spring and hot, humid summer conditions of southern China, this invention utilizes a multi-span greenhouse, using non-woven fabric seedling bags as root restrictors, filled with a cultivation substrate of peat moss, vermiculite, and perlite. 40-50 day old *Pyrus pyrifolia* or *Pyrus pyrifolia* seedlings are transplanted. Water and fertilizer are precisely controlled through a drip irrigation system, maintaining substrate humidity at 70%-80% after transplanting. Summer drip irrigation is used for supplemental watering, and the following spring, shoots are promoted. A suitable drought treatment is applied from mid-May to June to shorten the juvenile stage. Combined with temperature and humidity regulation and central leader shaping pruning, the seedlings are "shapely from the nursery stage." The pear seedlings cultivated using this invention for two years have an average height of 170cm, and the incidence of root rot is only 2.20%, a reduction of 89.7% compared to traditional cultivation, achieving standardized and intensive seedling production.