37 results about "Pyrus serotina" patented technology

The invention discloses a pyrus pyrifolia nakai enzyme and a preparation method and an application thereof. The pyrus pyrifolia nakai enzyme is prepared by the steps of pretreatment and fermentation with organic pyrus pyrifolia nakai and sugar as raw materials, and the total weight ratio of the organic pyrus pyrifolia nakai to the sugar is 1 to (1.2-2). Normal pressure and temperature natural fermentation is adopted, the fermentation process is stable, the fermentation is closer to the nature, the enzyme fermentation process endures severe cold and heat of the nature, the essence qi of the moon and the sun is fully absorbed, and the pyrus pyrifolia nakai is more nutritious. Therefore, a fermentation tank is provided with an independently developed breathing valve to balance the pressures inside and outside the fermentation tank; and at the same time, the fermentation tank is placed in the open air, and is fused together with the natural environment.

The invention relates to a thaumatin-like protein gene PpTLP from pyrus pyrifolia nakai with antifungal activity and application. The gene PpTLP has the base sequence shown in SEQID and coded thaumatin-like proteins. The invention verifies that the gene PpTLP has the function of improving the antifungal ability of the plants through correlation technique of functional genomics. When the antifungal gene PpTLP is constructed on a plant expression vector and is transformed into tobacco to be overexpressed, the transgenic tobacco has high antifungal activity. The protein from the transgenic tobacco expressing PpTLP has obvious inhibitory effects on various fungi such as sclerotinia sclerotiorum, phytophthora parasitica var. nicotianae, phomopsis sp. and the like.

The invention discloses compound ferment with smallanthus sonchifolius and pyrus pyrifolia nakai. The compound ferment comprises pyrus pyrifolia nakai ferment raw liquid and the smallanthus sonchifolius. The compound ferment disclosed by the invention has the beneficial effects that the fermentation speed can be accelerated, the nutritional components of the smallanthus sonchifolius can be more effectively extracted, the fermentation period can be reduced, the introduction of infectious microbes can be avoided, and the product safety can be improved.

The invention relates to a Pyrus pyrifolia polyploid induction method. The Pyrus pyrifolia polyploid induction method includes subjecting a single-bud stem segment of a Pyrus pyrifolia tissue culture seedling to polyploid induction with colchicine, performing differentiation culture and rooting culture after induction, and performing polyploid identification. The Pyrus pyrifolia polyploid induction method has the advantages of high induction directionality, low cost and simplicity in operation. The Pyrus pyrifolia polyploid induction method is a novel feasible efficient method for Pyrus pyrifolia polyploid breeding, and has significant application value in promoting artificial induction techniques for Pyrus pyrifolia polyploid in China.

The invention relates to a preparation method of fruit vinegar fermented from Lucid Ganoderma. The preparation method comprises the following steps: 1, selecting 70% of Lucid Ganoderma (wall broken Lucid Ganoderma) and 30% of millet, fermenting by a traditional fermentation process, extracting a fermentation broth; 2, mixing 20% of a Pyrus serotina juice, 20% of an apple juice, 20% of a sea buckthorn juice, 20% of an Actinidia chinensis juice and 20% of a tomato juice to obtain a mixed juice; 3, taking 500kg of purified water, adding 4% of the fermentation broth, 4% of the mixed juice and uniformly stirring according to a preparation proportion of 92%; and 4, adding relevant accessories, uniformly mixing, sterilizing, canning, sealing, cooling, and packaging. So the fruit vinegar is obtained.

The invention provides a method for in-vitro micro-grafting of tissue culture seedlings of Danshui pyrus pyrifolia, the method includes the steps of inoculating inducing medium with stem sections withbuds, serving as explants, to form sterile test tube plantlets through cultivation; transplanting the sterile test tube plantlets to proliferation medium to obtain rootless tissue culture seedlings through proliferation, and taking the proliferous rootless tissue culture seedlings as micro-grafting scions; inoculating seed germination medium with pyrus betulaefolia seeds to form stocks through cultivation; cutting the scions into wedged bases, putting the wedged bases into notches of the stocks to obtain micro-grafted seedlings; transplanting the micro-grafted seedlings into cultivation medium after domestication, and cultivating the seedlings in a plastic arched shed after transplanting, the method has the advantages that tissue culture and grafting techniques are combined, and the proliferous tissue culture seedlings and pyrus betulaefolia seedlings are subjected to micro-grafting, so that the induction process for rooting of the tissue culture seedlings is omitted, seedling cultureprocedures are simplified, seedling culture time is shortened, and resilience and high yield of nursery stocks are improved.

The invention relates to pyrus pyrifolia tree viscum coloratum grease with lung-clearing and phlegm-eliminating effects. The pyrus pyrifolia tree viscum coloratum grease is prepared from the following raw materials according to a weight ratio: 6 to 7 parts of radix astragali seu hedysari, 2 to 3 parts of semen brassicae, 2 to 3 parts of semen descurainiae, 2 to 3 parts of herba menthae, 1 to 2 parts of perillae fructus, 1 to 2 parts of bighead atractylodes rhizome, 3 to 4 parts of fresh ginger, 3 to 4 parts of apricot kernel, 2 to 3 parts of dried tangerine peel and 2 to 3 parts of gecko. The pyrus pyrifolia tree viscum coloratum grease has the advantages that the formula is reasonable and particular, and the grease is used for treating deficiency and cold type cough according to traditional Chinese medicine differentiation; the therapy effect is quick, the safety is realized, any side effect is avoided, and the like.

The invention discloses a method for producing a pyrus serotina drink. The method comprises the following steps: a, selecting and preparing materials, namely, peeling and removing kernels of collected fresh pyrus serotina, and performing washing and drying in air; b, performing deactivation color protection on the washed and dried pyrus serotina at 70 DEG C; c, performing low-temperature enzyme deactivation on the pyrus serotina after color protection; d, squeezing and breaking the pyrus serotina; e, discharging the pyrus serotina, and performing blending; f, performing homogeneous treatment; g, performing high-pressure sterilization; h, performing sterile filling; I, preparing a finished product. As no sodium metabisulfite is adopted for color protection, but the purpose of color protection is achieved by deactivation at 70 DEG C, no chemical substance is added, and moreover the enzyme deactivation temperature is relatively low, nutrient components such as vitamins can be not damaged.

The invention discloses a dandelion and pyrus pyrifolia Nakai composite ferment. The dandelion and pyrus pyrifolia Nakai composite ferment comprises pyrus pyrifolia Nakai ferment original fluid and dandelions. The dandelion and pyrus pyrifolia Nakai composite ferment disclosed by the invention has the beneficial effects that the fermentation speed can be increased, nutrient components of the dandelions can be effectively extracted, the fermentation period can be shortened, infectious microbes are prevented from being introduced, and the product safety is improved.

The invention discloses a coconut-milk folium-nelumbinis fragrant pecan kernel and a preparation method thereof. The coconut-milk folium-nelumbinis fragrant pecan kernel is made from the following raw materials: pecan kernel, coconut juice, purple cabbage, folium nelumbinis, porcine skin, astragalus polysaccharide, purple sweet potato, flos momordicae, folium pyrus bretschneideri / pyrus pyrifolia / pyrus ussuriensis, cortex moutan, rhizoma polygonati odorati, trehalose, wheat germ oil, ramulus cinnamomi, pine branch, bamboo charcoal, rice vinegar, medical stone powder and rice wine. According to the coconut-milk folium-nelumbinis fragrant pecan kernel made by the invention, fragrances of pine wood and cinnamomi wood permeate by a shell; the medical stone powder and bamboo charcoal powder are added to absorb a harmless substance; a quality guarantee period of the kernel is prolonged; meanwhile, a trace element of the kernel is added; moreover, by multi-layer heat conduction and transfer, unique fragrances of the kernel are retained; the kernel is more fragrant, strong, soft and crisp; extracted components of traditional Chinese medicines such as flos momordicae and rhizoma polygonati odorati are also added in a formula, are subjected to pulping with the raw materials such as astragalus polysaccharide to soak the pecan kernel; a healthcare function is intensified; an antibacterial effect is improved; the healthcare of an intestinal track is facilitated; meanwhile, the folium nelumbinis and a coconut fragrance are fused; the coconut-milk folium-nelumbinis fragrant pecan kernel not only strengthens brain and profits intelligence, but also can nourish yin, clear heat, lose a weight and beautify a look.

The invention discloses a honey and pyrus pyrifolia Nakai composite ferment. The honey and pyrus pyrifolia Nakai composite ferment comprises pyrus pyrifolia Nakai ferment original fluid and honey. Thehoney and pyrus pyrifolia Nakai composite ferment disclosed by the invention has the beneficial effects that the fermentation speed can be increased, nutrient components of the honey can be effectively extracted, the fermentation period can be shortened, infectious microbes are prevented from being introduced, and the product safety is improved.

The invention relates to a sour food and a making method thereof. Raw materials of the sour food comprise hawthorn fruits, wild Pyrus pyrifolia, salt, sugar, Emblica officinalis and Sanhua plum. The making method of the sour food comprises the following steps: 1, cleaning hawthorn fruits, wild Pyrus pyrifolia, Emblica officinalis and Sanhua plum for later use; 2, cutting the cleaned hawthorn fruits, wild Pyrus pyrifolia, Emblica officinalis and Sanhua plum to form blocks, and adding the blocks to a clean basin; 3, uniformly scattering salt in the basin, and stirring for 2-5min; and 4, uniformly scattering sugar in the basin, stirring for 2-5min, and preserving for 5-20min after sugar and salt are dissolved in order to obtain the sour food which can be instantly eaten. The sour food has the characteristics of wide material drawing, many kinds of fruits, and simple formula; and the making method is simple, reserves the freshness and deliciousness of the fruits, and meets people's different taste demands.

The invention discloses a turmeric and pyrus pyrifolia nakai enzyme complex, which is prepared from pyrus pyrifolia nakai enzyme and turmeric. The enzyme complex has the beneficial effects of accelerating the fermenting speed, more effectively extracting nutritional ingredients of turmeric, reducing the fermenting cycle, avoiding introducing contaminating microorganisms and improving product safety.

The invention relates to a preparation method of a plant amino-acid drink. The preparation method is characterized in that: selecting black sesame as a main ingredient, performing fermentation technology to obtain black sesame juice, mixing with a mixed extracted concentrated liquor of various mushrooms such as lentinus edodes or flower mushroom, poria cocos, ramie seed, phyllanthus emblica and the like, and mixing with sea-buckthorn powder, rosa roxburghii powder and donkey-hide glue liquor, and finally mixing with concentrated juice of gold-bead pyrus pyrifolia, adding auxiliary materials and uniformly stirring, sterilizing and refrigerating to obtain the drink.

A complete remedy for AIDS is difficult to obtain. As such, a useful process was designed to search for an anti-HIV1 agent that has an immuno response modification activity capable of releasing immuno suppression, activating killer cells to destroy persistent infection cells, elevating antibody titer to activate ADCC activity, and vice versa.The process consists of 4 elements: guinea pig or mouse peritoneal derived adherent macrophages / monocytes as effector cells; cyclophosphamide as an immuno suppressor; chicken RBC as target cells; and the anti-HIV1 agent candidate to be examined.Immunovir and components were isolated from Pyrus serotina Rehder and other species of Rosaceae by column chromatography.Another useful process is the comparison of fluorescent antibody titer patterns among one round, two round, and non-medicated infected monkeys.The results of such processes can show that the anti-HIV1 agent, such as these immunovirs, are noble candidates for the complete remedy of AIDS.

The invention discloses plant pyrus pyrifolia Nakai. fragrant oral cavity atomized liquid and a preparation method. The preparation method comprises the following steps of S1, purchasing qualified pyrus pyrifolia Nakai., a sodium chloride solution of 0.9%, liquor nitrofurazoni of 0.02%, radix scutellariae, Chinese cinnamon, rhizoma cimicifugae, mint and lemons from regular shops; S2, cleaning components of the pyrus pyrifolia Nakai., the radix scutellariae, the Chinese cinnamon, the rhizoma cimicifugae, the mint and the lemons, and after cleaning, placing the cleaned components in a drying room for drying; and S3, respectively crushing the pyrus pyrifolia Nakai., the radix scutellariae, the Chinese cinnamon, the rhizoma cimicifugae, the mint and the lemons, which are dried in S2, after mashing, performing screening with a screen mesh, and preparing fine powder. The plant pyrus pyrifolia Nakai. fragrant oral cavity atomized liquid is simple to make, the sodium chloride solution of 0.9%and the liquor nitrofurazoni of 0.02% are added, so that the effect of sterilizing can be achieved, and the plant pyrus pyrifolia Nakai. fragrant oral cavity atomized liquid is good in oral cavity cleaning effect; and the pyrus pyrifolia Nakai., the radix scutellariae, the Chinese cinnamon, the rhizoma cimicifugae, the mint, the lemons and the like are also added, so that the smell is fresh, people feel comfortable when smelling the plant pyrus pyrifolia Nakai. fragrant oral cavity atomized liquid, and the plant pyrus pyrifolia Nakai. fragrant oral cavity atomized liquid is free from side effects.

The invention discloses a pyrus pyrifolia jam powder processing method. By adoption of pyrus pyrifolia as a main material, the method includes steps of raw material pretreatment, pulping, enzyme treatment, compounding, homogenizing, lyophilizing, grinding, filtering and the like. Pyrus pyrifolia jam powder prepared according to the method is stable in color through sulphurous acid treatment; the pectin content of raw materials can be reduced by enzymolysis, more nutrient substances can be separated out, and the raw material utilization rate is increased; due to freeness of any artificial agents, the natural flavor of pyrus pyrifolia can be maintained, and efficacies of lung moistening, cough arresting, beautifying, detoxifying and senescence delaying are achieved.

The invention discloses a specific molecular marker of a pear PbELF3b gene and an application of the specific molecular marker. Clonal PbELF3b allele N type and D type and a modified N-d type and D+dtype are separated from pyrus pyrifolia and pyrus sinkiangensis, respectively; the D type misses a d fragment of 58 bp in size relative to the N type, and deletion of the fragment can lead to strongerdelayed flowering activity of the PbELF3b gene; an InDel sequence-specific marker primers SEQ ID NO:4 and SEQ ID NO:5 designed based on intron deletion fragments can be used as molecular markers to identify the differentiation and development situations of pear flower buds, so that the breeding efficiency can be improved; and different types of the PbELF3b can be used for regulation and control of flowering time of other plant species by using a genetic engineering process.

The present invention relates to vanadium-enriched black olive boiled dumplings. The boiled dumplings are prepared from the following raw materials: vanadium-enriched konjac flour, vanadium-enriched buckwheat flour, dragon fruits, black olives, viscum pyrus serotina trees, viscum pine trees, shii-take, pork and edible salt. The boiled dumplings are rich in tastes, attractive in color, high in nutritional value, and rich in anthocyanins, and have efficacies of preventing oxidation, lowering blood pressure and blood fat, and enhancing immune system.

The invention relates to the technical field of pear tree cultivation, in particular to a pear tree cultivation method for preventing pear tree trunk diseases, which comprises the following steps: selecting pyrus pyrifolia which is 1.5 m or more in seedling height, about 1.0-2.0 cm in stem thickness, good in adaptability and strong in pear tree branch and trunk disease resistance as stock seedlings; the method comprises the following steps: cutting a scion on a rootstock seedling, placing the cut surface of the scion in a mixed solution of 0.03-0.06% forchior fennron and 0.05-0.07% moonlight flower element, quickly dipping, grafting on the rootstock seedling at a high position by adopting a cut grafting method, spraying a grafting promoting solution at the joint of the scion and the rootstock, and preparing the grafting promoting solution from amino-oligosaccharin, a fructus psoraleae seed extract, vitamin B12 and deionized water. The grafting survival rate of the pear trees can be remarkably increased, and the occurrence rate of trunk diseases in the pear tree cultivation process is reduced.

A useful HIV remedy process consists of 4 elements: guinea pig or mouse peritoneal derived adherent macrophages / monocytes as effector cells; cyclophosphamide as an immuno suppressor; chicken RBC as target cells; and the anti-HIV1 agent candidate to be examined.Immunovir and components were isolated from Pyrus serotina Rehder and other species of Rosaceae by column chromatography.

The invention relates to a preparation method of pyrus pyrifolia paste. The pyrus pyrifolia paste is mainly prepared through decocting wild medical pears of pyrus pyrifolia, besides, in the decoctioncourse, an appropriate quantity of traditional Chinese medicine materials of fructus momordicae, radix rehmanniae, radix puerariae, fritillary bulbs and the like are further added for decoction together. The materials have different health-care effects respectively, so that the efficacy of the pyrus pyrifolia paste is better than that of traditional pear syrup, the pyrus pyrifolia paste has the main efficacy of moistening lung for arresting a cough, promoting salivation and relieving sore throat, is used for treating cough and dyspnea with rapid and short breath caused by yin deficiency and lung heat, viscous phlegm saliva, fullness and oppression in the chest and diaphragm, dry mouths, dry throat, dysphoria and hoarseness, and is particularly excellent for patients suffering from asthma,and patients suffering from impairment of yin due to lung heat and enduring cough. The preparation method is simple to operate, and pyrus pyrifolia paste has the advantages of being obvious in effectsand like, and is suitable for large-scale popularization and application.

The invention discloses a Pyrus serotina tea. The tea is made from, by weight, 10-15 parts of Pyrus serotina, 2-3 parts of honeysuckle flowers, 3-4 parts of Chinese olives, 1-2 parts of mint leaves, 10-20 parts of rock sugar, 1-2 parts of Common Sage Herb, 1-2 parts of Solanum nigrum, 3-5 parts of wolfberry fruits, 2-4 parts of red dates and 3-4 parts of chrysanthemum flowers. The invention provides the Pyrus serotina tea having the functions of moistening the lung and relieving cough, clearing heat and detoxifying, and invigorating the brain and developing intelligence, and a making method thereof.

The invention relates to a thaumatin-like protein gene PpTLP from pyrus pyrifolia nakai with antifungal activity and application. The gene PpTLP has the base sequence shown in SEQID and coded thaumatin-like proteins. The invention verifies that the gene PpTLP has the function of improving the antifungal ability of the plants through correlation technique of functional genomics. When the antifungal gene PpTLP is constructed on a plant expression vector and is transformed into tobacco to be overexpressed, the transgenic tobacco has high antifungal activity. The protein from the transgenic tobacco expressing PpTLP has obvious inhibitory effects on various fungi such as sclerotinia sclerotiorum, phytophthora parasitica var. nicotianae, phomopsis sp. and the like.

The invention relates to a preparation technology for a double pear fruit vinegar beverage. The technology is characterized by: selecting Pyrus pyrifolia juice and Rosa roxburghii juice, mixing the two juice with Houttuynia cordata, lonicera japonica and Rorippa indica, extracting the concentrated solution, adding purified water, vinegar and other related auxiliary materials, carrying out blending, sterilization, canning, sealing and packaging, thus obtaining the double pear fruit vinegar beverage.

The invention relates to the technical field of agricultural planting maintenance, in particular to a row spacing control device for pyrus pyrifolia. The row spacing control device for pyrus pyrifoliacomprises a frame and a cutting device, and the cutting device is arranged on the frame; the frame is composed of a clamping ring, connecting rods and a connecting disc, the connecting rods are arranged on the clamping ring, and the connecting disc is arranged on the connecting rods; the cutting device is composed of a handle, a sliding block, a cutting plate, a rotating disc, a rotating shaft and a bearing, the sliding block is arranged on the handle, the cutting plate is arranged on the sliding block, the rotating disc is arranged on the cutting plate, the rotating shaft is arranged on therotating disc, and the bearing is arranged on the rotating shaft. The row spacing control device for pyrus pyrifolia is high in practicality and quite convenient to use; pyrus pyrifolia saplings of planters and planting enterprises can be transplanted according to the actual condition of planting land during dense planting, and the row spacing and plant spacing between pyrus pyrifolia saplings arereasonably controlled; besides, the labor intensity of planters and planting enterprise workers is reduced, the loss of enterprises is reduced, and the benefit of enterprises is increased.

The present invention relates to a Suanye and a preparation method thereof, wherein the raw materials of the Suanye comprise wild hawthorn, wild pyrus pyrifolia, salt, sugar, phyllanthus emblica linn fruit and wild pomegranate. The preparation method comprises: 1) washing wild hawthorn, wild pyrus pyrifolia, phyllanthus emblica linn fruit and wild pomegranate so as to be spare; 2) cutting the washed materials into blocks, and placing into a clean basin; 3) uniformly sprinkling the salt into the basin, and stirring for 2-5 min; and 4) uniformly sprinkling sugar into the basin, stirring for 2-5 min, sousing for 5-20 min after the sugar and the salt are dissolved so as to eat. According to the present invention, the Suanye has characteristics of wide raw material source, more fruit variety, simple formula and simple preparation method, retains the delicious taste of the fruits, and can meet different taste requirements of people.

The invention relates to a cultivation method for inhibiting pericarp rust spots of P. pyrifolia Nakai, which comprises the steps of conducting pest and disease control; carrying out physical cleaningon the surface of young fruits; forming a biological protective film on the young fruits of P. pyrifolia Nakai; and performing fertilizer and water management in the later period of fruit development. The cultivation method is significant in inhibition effect for the pericarp rust spots of P. pyrifolia Nakai. The foxiness index of the pericarp of P. pyrifolia Nakai obtained according to the method is reduced by 70%-80% than the foxiness index of the pericarp of open field rust spot P. pyrifolia Nakai, the cleanliness of the pericarp is significantly improved, and the appearance quality is greatly improved, thereby solving the defect that the poor appearance of rust spot P. pyrifolia Nakai varieties affects the commercial value of the fruit. The cultivation method is low in cost and convenient to master, and provides a reference basis for improving the appearance quality of excellent pear varieties.