15 results about "Pyrus serotina" patented technology

The invention discloses an application of a PpWRKY53 transcription factor in improving heat resistance of Chinese pear fruits, the transcription factor is a positive regulatory factor of Chinese pears responding to high temperature stress, overexpression of the transcription factor PpWRKY53 gene in the Chinese pear fruits can significantly reduce hydrogen peroxide accumulation and membrane lipid peroxidation caused by high temperature, effectively alleviate water stain type browning symptoms of the fruits, and improve the heat resistance of the Chinese pears. The fruit hardness is improved. The invention provides a key gene resource for creating new germplasm of heat-resistant Chinese pears and breeding new varieties with high-temperature-resistant characteristics, and has important scientific and application values for ensuring stable production of summer Chinese pear industry and improving fruit quality and economic benefits.

The invention discloses an application of a single nucleotide polymorphism site as a detection target in preparation of a kit for detecting the early flowering stage character of pears, and belongs to the technical field of plant molecular markers, the single nucleotide polymorphism site is that the 247th site of a nucleotide sequence shown as SEQ ID NO.1 has polymorphism of C > T, and the 247th site of the nucleotide sequence shown as SEQ ID NO.2 has polymorphism of C > T; the single nucleotide polymorphism site is used as a detection target spot for detecting the early flowering stage character of the pear; the method is rapid, simple and convenient, has high specificity, can realize high-throughput detection, and has important significance in early flowering stage variety identification, new variety breeding, breeding period shortening and breeding efficiency improvement.

This invention discloses the application of the PbeARF2 gene, an ARF-type transcription factor in *Pyrus pyrifolia*, belonging to the field of bioengineering technology. The nucleotide sequence of the PbeARF2 gene is shown in SEQ ID NO.1. The applications include enhancing plant resistance to iron deficiency stress, promoting root acidification, increasing the activity of ferric reductase under iron deficiency stress, cultivating plant varieties with high resistance to iron deficiency stress, and increasing chlorophyll content in leaves under iron deficiency stress. This invention, through biological experiments, demonstrates for the first time the function of the PbeARF2 ARF-type transcription factor gene and its encoded protein in promoting iron absorption in plants under iron deficiency stress, laying a theoretical foundation for plant variety improvement and the creation of plant varieties with high iron deficiency resistance.

This invention provides a cold-resistance gene containing a BRZL domain isolated and cloned from pear rootstock. PbbZIP44 This study also provides insights into the application of this gene in enhancing plant cold resistance. Through the construction of... PbbZIP44 Gene overexpression and silencing vectors were developed and introduced into Arabidopsis thaliana, *Pyrus pyrifolia* callus, and *Pyrus pyrifolia* seedlings using Agrobacterium-mediated genetic transformation. Stable and transient expression were achieved, resulting in stable overexpressing plants and TRV-mediated silencing lines for low-temperature resistance functional analysis. Results showed that under low-temperature stress, the overexpressing lines exhibited stronger growth, lower malondialdehyde content and relative conductivity, as well as higher photosynthetic efficiency and antioxidant capacity compared to wild-type plants; while the silencing lines showed the opposite physiological response.

The invention relates to the technical field of plant disease-resistant gene identification and genetic engineering, in particular to a pyrus betulaefolia lectin receptor-like kinase gene sequence and application of the pyrus betulaefolia lectin receptor-like kinase gene sequence to improvement of apple and pear rot disease resistance. The pyrus betulaefolia canker-resistant gene sequence provided by the invention is used for improving the ability of resisting saprophytic fungus canker. The pyrus betulaefolia anti-disease gene provided by the invention is obtained by cloning from a pyrus betulaefolia L-LecRKs family for the first time, the function and possible action mechanism of the anti-disease gene PbeL-LecRK-S.4 in the aspect of disease resistance are further studied, and a theoretical basis is provided for breeding of pyrus betulaefolia for disease resistance. According to the invention, a pFGC5941-PbeL-LecRK-S.4 overexpression vector and a PTRV2-PbeL-LecRK-S.4 silence vector are constructed for the first time, and the pFGC5941-PbeL-LecRK-S.4 overexpression vector and the PTRV2-PbeL-LecRK-S.4 silence vector are introduced into pyrus betulaefolia ('Duli-G03') cells through transient expression verification of Huangguan pear fruits and agrobacterium tumefaciens-mediated transformation. The disease-resistant response of a pyrus betulaefolia disease-resistant gene PbeL-LecRK-S.4 overexpression suspension cell line and wild pyrus betulaefolia suspension cells after inoculation of the rot disease is studied. The pyrus betulaefolia anti-disease gene PbeL-LecRK-S.4 provided by the invention can obviously improve the resistance of pyrus betulaefolia to valsa ceratosperma.

The invention discloses an accurate monitoring and efficient prevention and treatment system for black spot of dwarf and close-planting bergamot pear trees, which belongs to the technical field of prevention and treatment of black spot of bergamot pear trees and comprises a prevention system and a treatment system for black spot of bergamot pear trees. According to the precise monitoring and efficient prevention and treatment system for the dwarf densely-planted fragrant pear tree black spot, precise monitoring and timely and targeted pesticide spraying of the dwarf densely-planted fragrant pear tree black spot can be achieved, so that the efficient prevention and treatment effect is achieved, the pesticide use amount can be greatly reduced, the production cost of fruit farmers is reduced, and the economic benefits of the fruit farmers are improved; the produced bergamot pears are small in pesticide residue, the quality of fruits which are not invaded by diseases is better, and the commodity value is higher; the damage of pesticide control to the ecological environment can be reduced, and the living conditions of surrounding residents are improved.

1. Name of the designed product: bath ball (quince). 2. Use of the designed product: for bathing. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: perspective view.

The invention provides a method for constructing a stable genetic transformation system of Chinese pears, and belongs to the technical field of genetic transformation system construction. A construction method of a Chinese pear stable genetic transformation system comprises the following steps: infecting in-vitro petioles of Chinese pears by using an agrobacterium solution containing a target gene segment, co-culturing the infected Chinese pear in-vitro petioles, removing bacteria, screening, culturing, subculturing and identifying to obtain positive and stable genetic Chinese pear tissue culture seedlings. The technical scheme provided by the invention accelerates the research of the Chinese pear gene function, and has important significance for accelerating the genetic improvement work of Chinese pears.

1. Name of the designed product: storage cylinder (duck pear). 2. Use of the designed product: storage cylinder for storing articles. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: perspective view.

This invention discloses a method for establishing a high-efficiency regeneration system for *Pyrus pyrifolia* leaves, belonging to the field of plant tissue culture technology. The method includes: spreading a layer of sorbitol solution on the surface of a culture medium; inoculating *Pyrus pyrifolia* seedlings into the medium and placing them in a dark environment for etiolation treatment; taking etiolated leaves from the *Pyrus pyrifolia* seedlings and inoculating them in a regeneration medium for dark culture, then transferring them to light for further culture to produce adventitious buds; after the adventitious buds reach the target length, cutting off their lateral buds and placing them on a subculture medium to obtain regenerated seedlings. This invention, by adding sorbitol solution before dark treatment, can significantly shorten the time to obtain etiolated material to 7 days, and the etiolated plants are in better condition after three weeks; furthermore, by placing *Pyrus pyrifolia* tissue culture seedlings in a dark environment for dark culture, the physiological state of the tissue culture seedlings is altered, and the obtained etiolated leaves are inoculated into the regeneration medium, improving the regeneration efficiency, and the material acquisition is not affected by the season.

This invention discloses a pear resistance to ring rot disease-related protein, its encoding gene, and its applications. The amino acid sequence of the pear resistance to ring rot disease-related protein is shown in SEQ ID NO:1. The gene encoding the pear resistance to ring rot disease-related protein, PbrTLP15, has a nucleotide sequence shown in SEQ ID NO:2. Overexpression of PbrTLP15 enhances the plant's resistance to *B. dothidea*. Virus-induced gene silencing (VIGS) revealed that silencing PbrTLP15 reduces the resistance of pear plants to *B. dothidea*. These results all indicate that PbrTLP15 possesses anti-*B. dothidea* activity. This invention provides a theoretical basis for studying the role of pear PbrTLP15 in resistance to ring rot and exploring the molecular mechanism of pear disease resistance, and also provides new insights and resources for breeding pear varieties resistant to ring rot.

This invention provides a method for semi-restricted root cultivation of pears in southern China, belonging to the field of fruit tree seedling technology. Addressing the problems of uncontrolled root growth, susceptibility to root rot, and prolonged juvenile stage associated with traditional seedling cultivation in the rainy spring and hot, humid summer conditions of southern China, this invention utilizes a multi-span greenhouse, using non-woven fabric seedling bags as root restrictors, filled with a cultivation substrate of peat moss, vermiculite, and perlite. 40-50 day old *Pyrus pyrifolia* or *Pyrus pyrifolia* seedlings are transplanted. Water and fertilizer are precisely controlled through a drip irrigation system, maintaining substrate humidity at 70%-80% after transplanting. Summer drip irrigation is used for supplemental watering, and the following spring, shoots are promoted. A suitable drought treatment is applied from mid-May to June to shorten the juvenile stage. Combined with temperature and humidity regulation and central leader shaping pruning, the seedlings are "shapely from the nursery stage." The pear seedlings cultivated using this invention for two years have an average height of 170cm, and the incidence of root rot is only 2.20%, a reduction of 89.7% compared to traditional cultivation, achieving standardized and intensive seedling production.

The invention belongs to the technical field of molecular biology, and particularly discloses application of a pear source PcMIR397b promoter in construction of a pear instantaneous transformation vector. The pear source PcMIR397b promoter which is high in expression efficiency and wide in tissue compatibility is screened and identified, the capacity of the promoter for driving target gene expression in a pear isolated organ is remarkably superior to that of a common heterologous promoter and a common endogenous promoter, and the pear source PcMIR397b promoter can efficiently play a role in different parts such as stems and leaves. The invention also provides an instantaneous transformation method of a pear plant, which combines the endogenous efficient pear source PcMIR397b promoter with a traditional agrobacterium-mediated transformation process, breaks through the limitation of application of a heterologous promoter in pears, and constructs an instantaneous transformation system which is more adaptive to pear species, convenient to popularize and use, rapid and efficient. The method is suitable for rapid verification of pear gene functions, and provides key technical support for improvement of pear gene editing efficiency and acceleration of a high-quality breeding process.