2 results about "Etiolation" patented technology

A method for root-cutting propagation and seedling cultivation and management of Aralia echinocaulis, which method comprises: selecting lateral horizontal roots of a 2- to 3-year-old robust mother plant to prepare cuttings; soaking the cuttings in a compound solution of indolebutyric acid, naphthaleneacetic acid and 6-benzylaminopurine; performing horizontal burying of cuttings using a sterilized composite matrix composed of peat soil, perlite and humus soil at a ratio of 3:2:1; precisely regulating the temperature and humidity, light and matrix water content in an intelligent temperature-controlled greenhouse; applying nutrient solutions with specific nitrogen-phosphorus-potassium ratios in stages, combined with ferrous sulfate and boric acid to prevent etiolation and promote rooting; gradually adjusting environmental parameters to enter a management stage after adventitious buds unfold leaves; and performing gradient seedling hardening after seedlings achieve the standard.

This invention discloses a method for establishing a high-efficiency regeneration system for *Pyrus pyrifolia* leaves, belonging to the field of plant tissue culture technology. The method includes: spreading a layer of sorbitol solution on the surface of a culture medium; inoculating *Pyrus pyrifolia* seedlings into the medium and placing them in a dark environment for etiolation treatment; taking etiolated leaves from the *Pyrus pyrifolia* seedlings and inoculating them in a regeneration medium for dark culture, then transferring them to light for further culture to produce adventitious buds; after the adventitious buds reach the target length, cutting off their lateral buds and placing them on a subculture medium to obtain regenerated seedlings. This invention, by adding sorbitol solution before dark treatment, can significantly shorten the time to obtain etiolated material to 7 days, and the etiolated plants are in better condition after three weeks; furthermore, by placing *Pyrus pyrifolia* tissue culture seedlings in a dark environment for dark culture, the physiological state of the tissue culture seedlings is altered, and the obtained etiolated leaves are inoculated into the regeneration medium, improving the regeneration efficiency, and the material acquisition is not affected by the season.