98 results about "Ferric citrate" patented technology

The invention discloses a preparation method and application of monatomic iron-doped carbon quantum dot nano enzyme, and the preparation method comprises the following steps: fully dispersing citric acid, urea and iron salt, and fully reacting in a closed container; the ferric salt is any one of ferric citrate, iron methionine or ferrous lactate. The monatomic iron-doped carbon quantum dot nano-enzyme provided by the invention is good in dispersity, excellent in water solubility and uniform and stable in structure, has efficient antioxidant enzyme activity, can be easily absorbed by plants, can remarkably improve the antioxidant capacity and stress resistance of the plants after being applied, can remove excessively accumulated active oxygen of the plants, and also can effectively inhibit the growth of the plants. The soil heavy metal pollution control agent can control soil heavy metal pollution, also can improve the removal efficiency of antioxidant enzymes in plants, reduce heavy metal absorption and increase the plant yield, has the characteristics of small dosage, low cost, high efficiency, high environmental safety and easy use, and provides a new technology and product choice with practical application value for treatment of soil heavy metal pollution.

The invention relates to application and a method of phosphorus adsorption resin for selectively adsorbing phosphorus from phosphorus-containing wastewater. The phosphorus adsorption resin comprises weak acid cation exchange resin and Fe < 3 + > loaded on the surface of the weak acid cation exchange resin; the pH value of the phosphorus-containing wastewater is 0.8-3.0, the phosphorus concentration is 0.1-5 g / L, and the wastewater further contains 0.1-1 g / L of Ca; the phosphorus adsorption resin is prepared by the following steps: i) carrying out contact reaction on weak acid cation exchange resin and a ferric salt solution, wherein the ferric salt is selected from one or more of ferric sulfate, ferric chloride, ferric nitrate, ferric citrate and ferric tartrate; and ii) after the reaction is completed, carrying out solid-liquid separation to obtain the Fe < 3 + >-loaded phosphorus adsorption resin. According to the method, high-selectivity adsorption (larger than or equal to 95%) of phosphorus can be achieved, the phosphorus and calcium separation effect is good, the iron-calcium ratio in the phosphorus-enriched strip liquor is larger than or equal to 800, and the method can be used for preparing battery-grade iron phosphate.

The invention discloses a method for inducing a regenerated plant based on a small spike stalk of bamboo-stalk, and relates to the technical field of regenerated plants.The method comprises the steps that the small spike of flowering bamboo-stalk is taken, disinfected, washed with sterile water and placed on sterile filter paper to absorb moisture, and dissected to obtain the small spike stalk as an explant; the small spike stalks are inoculated into a callus induction culture medium and cultured for 1-2 weeks under the dark condition, callus is formed through induction, and the induction culture medium comprises an MS culture medium, 2, 4-D, KT, ferulic acid, aspartic acid, Yb, an amino acid mixture and cane sugar; the callus is inoculated into a differential culture medium and cultured under the illumination condition, adventitious buds are differentiated, and the rooting culture medium comprises a 1 / 2 MS culture medium, IBA, NAA, ferric citrate, beta-salicylic acid and cane sugar. According to the method disclosed by the invention, a callus induction system starting from reproductive tissues of the bamboo-stalk bamboo is established, regenerated plants are successfully obtained, and a technical means is provided for rapid propagation of bamboo-stalk bamboo seedlings.

The invention discloses a method for promoting biomass accumulation of haematococcus pluvialis, which comprises the following steps: adding salicylic acid, melatonin and alpha-ketoglutaric acid into a basic culture medium, and replacing ferric trichloride with ferric citrate as an iron source to obtain an improved BG-11 culture medium; the method comprises the following steps: inoculating a haematococcus pluvialis seed solution into a culture bottle containing an improved culture medium, carrying out illumination treatment on the first day to the second day by adopting pulsed red light with the wavelength of 620 nm in the early stage of a green stage, carrying out illumination treatment on the third day to the fifth day by adopting red light with the wavelength of 660 nm in the middle stage of the green stage, and carrying out illumination treatment on the sixth day to the seventh day by adopting pulsed red light with the wavelength of 680 nm in the later stage of the green stage. Through a synergistic scheme of the improved BG-11 culture medium, staged red light treatment and dynamic environment regulation and control, the problems of low light energy utilization rate and insufficient stress resistance in the existing haematococcus pluvialis culture process are solved, high efficiency and stability of haematococcus pluvialis biomass accumulation are realized, the biomass can reach 7.0 * 10 < 5 > cells / mL in the later period of the green stage, and the yield of the haematococcus pluvialis can reach 7.0 * 10 < 5 > cells / mL in the later period of the green stage. And the survival rate of haematococcus pluvialis cells reaches 95%.

The invention provides a method for producing beta-glucuronidase from escherichia coli and application of the beta-glucuronidase, and relates to the technical field of microorganisms. The method provided by the invention comprises the following steps: inoculating Escherichia coli ATCC 25922 on a composite culture medium for pre-culture, and culturing in an electric field environment of 1-10N / C for 12-24h to obtain a mixture containing beta-glucuronidase; wherein the composite culture medium comprises 10-100 [mu] mol / L of ferric citrate, and the concentration of the ferric citrate is increased in stages in the pre-culture process. According to the method, the escherichia coli ATCC 25922 is cultured in the iron-containing culture medium and the low-intensity electric field environment, so that the synthesis of the beta-glucuronidase by the escherichia coli ATCC 25922 can be effectively improved, and the enzyme activity and the enzyme yield of the beta-glucuronidase are improved.

The invention discloses a method for immobilization and magnetic separation of arsenic in water by biomineralization, which comprises the following steps: S1, preparation of a liquid culture medium: dissolving a 60% (volume) sodium lactate solution, sodium nitrate, potassium dihydrogen phosphate, magnesium sulfate heptahydrate and a yeast extract in deionized water in proportion, adjusting the pH value of the culture medium solution to 6.9-7.0, sub-packaging and performing high-pressure sterilization; s2, inoculating a bacteria solution into the sterilized culture medium for amplification culture; s3, preparing a standard arsenic solution; s4, preparing ferric citrate and ferrous chloride solutions; s5, performing MSR-1 biological control mineralization synthesis of magnetosome; 6, MSR-1 induces ferrous iron to precipitate and remove arsenic; and S7, magnetically separating a bacterium-iron-arsenic mixture. The method realizes fixation and separation of As in water, not only retains the advantages of low cost and environmental friendliness of biomineralization polluted water remediation, but also overcomes the defects of low remediation efficiency and potential pollution caused by incapability of recycling.

The method is characterized in that in the early March to the early April in the Korla region of Xinjiang, a certain amount of ferric citrate aqueous solution is instilled into the trunk of a Korla pear plant suffering from yellows in the last year by adopting an instillation method, and the dilution ratio of ferric citrate is 0.04 wt%-0.06 wt%. Through agent screening and application method screening, it is believed that the correction effect of the current ferric citrate instillation method on the yellows is obviously superior to that of the traditional soil application and ferrous sulfate spraying methods. Compared with the prior art, after the ferric citrate is applied, the green recovery speed is higher than that of a special medicament 'Ligutai' for etiolation in the market.

This invention discloses a culture medium for manganese-oxidizing bacteria suitable for low-carbon source environments in water supply networks and its application. The culture medium is prepared by mixing ferric ammonium citrate, manganese sulfate, ammonium sulfate, sodium chloride, magnesium sulfate, dipotassium hydrogen phosphate, and sodium nitrate, followed by high-temperature and high-pressure sterilization to obtain a culture solution. Manganese sulfate is added to the culture solution to obtain the manganese-oxidizing bacteria culture medium. Manganese-oxidizing bacteria are screened using this culture medium. Cast iron pipe scale is subcultured in the medium to enrich the manganese-oxidizing bacteria culture solution. The enriched manganese-oxidizing bacteria culture solution is diluted and spread onto agar solid medium, and incubated at a constant temperature to obtain manganese-oxidizing bacteria colonies. This invention solves the problem of altered bacterial physiological metabolism caused by high concentrations of added manganese ions, effectively improving bacterial activity and manganese oxidation capacity.

The invention relates to a carbitol-containing anti-caking water-soluble fertilizer and a preparation method thereof, and belongs to the field of water-soluble fertilizer production, the carbitol-containing anti-caking water-soluble fertilizer comprises the following substances by weight: 0.15-0.2% of carbitol, 20-28% of diammonium hydrogen phosphate, 22-28% of ammonium nitrate, 40-50% of potassium sulfate, 3-5% of magnesium nitrate, and 2-4% of ferric citrate. According to the invention, carbitol is applied to anti-caking of the water-soluble fertilizer for the first time, and integration of anti-caking, stability improvement and function synergism is realized; by adding carbitol, caking of the water-soluble fertilizer can be effectively avoided, a green and efficient technical scheme is provided for improving the quality of the water-soluble fertilizer, and the storage and use cost is reduced.

The invention relates to the technical field of cell culture media, in particular to a sheep testis somatic cell culture medium and a culture method.The sheep testis somatic cell culture medium is prepared from, by weight, 10-15 parts of MEMS alpha, 4-7 parts of KSR, 2-4 parts of sodium chloride, 3-5 parts of recombinant human transferrin, 1-3 parts of ferric citrate, 2-4 parts of L-glutamine, 5-10 parts of water and 4-7 parts of hydroxyapatite. According to the cell culture medium, MEM and KSR are adopted as matrix raw materials, sodium chloride and other auxiliaries are added, the hydroxyapatite agent is matched, the performance change of the cell culture medium on the survival rate and the survival amount of testicular somatic cells is enhanced, meanwhile, hydroxyapatite serves as a matrix of the hydroxyapatite agent, the performance of the cell culture medium is improved through a specific modification solution, and the cell culture medium is prepared. And the function of hydroxyapatite blended kaolin in the system is optimized, so that the functional effect of the cell culture medium is enhanced, and the performance of the product is further improved.

In an iron (II) citrate monohydrate, identified by the formula FeC6H6O7-H2O ( Ferrous citrate monohydrate or iron citrate (II) monohydrate), obtained according to a particular production method, citric acid and elemental iron are bound in a molecular complex where the iron is agglomerated in the citrate which moves to a cyclic form and the iron is bound to the O groups of the carboxylic groups 1 and 3 through ionic bonds, forming two enantiomers, D and L (R and S), both perfectly water-soluble and highly assimilable by the human body. Formula (L) & Formula (D). With a different absorption mechanism from traditional iron molecules, said iron citrate monohydrate is usable in pharmaceutical and / or nutraceutical and / or food compositions, in particular in the treatment and prevention of iron deficiency anemias and in general of all diseases and disorders associated with iron deficiency. The effectiveness is proven by several examples.

Provided are a perfusion culture method and the like that are able to improve the productivity in the production of recombinant protein. Specifically, a method for improving recombinant protein production in perfusion culture of an animal cell having a capacity to produce a recombinant protein, the method comprising adding cystine and / or ammonium iron citrate to a cell culture medium, and the like, are provided.

The present invention relates to the technical field of daily chemicals, specifically to an essence with whitening, beauty-enhancing and radiation-resistant functions and a preparation method thereof. Ferric citrate is added to the essence prepared by the present invention, and ferric citrate can enhance the antioxidant capacity of the skin, reduce the damage of oxygen free radicals to the skin, maintain the elasticity and luster of the skin, and achieve the effect of beauty. Soybean polysaccharides are natural and environmentally friendly, can remove free radicals, and improve cell vitality and cell radiation tolerance. Taxifolin has good radiation resistance and antioxidant properties. The essence with whitening, beauty-enhancing and radiation-resistant functions provided by the present invention adopts a variety of natural active ingredients, has good biocompatibility and safety, can effectively improve skin quality, and protect the skin from radiation and oxidation damage.

The invention discloses a nitrogen-phosphorus-boron-iron co-doped coconut shell activated carbon carrier and a preparation method and application thereof, the method comprises the following steps: S1, crushing coconut shell activated carbon, and carrying out Ar / water vapor activation treatment to obtain pretreated activated carbon; s2, after the chitosan solution and the urea solution are mixed, phytic acid is added, the pH is adjusted to 5.5-6.0, stirring is conducted, and a wetting agent is obtained; s3, adding a wetting agent into the pretreated activated carbon, heating, stirring, filtering, washing, and drying in vacuum to obtain pre-activated activated carbon containing nitrogen and phosphorus; and S4, dissolving ammonium ferric citrate and sodium citrate in deionized water, adding ethylene glycol, stirring, then adding the pre-activated activated carbon containing nitrogen and phosphorus, heating, stirring again, then dropwise adding a potassium borohydride mixed solution, and roasting to obtain the nitrogen, phosphorus, boron and iron co-doped coconut shell activated carbon carrier. After the nitrogen-phosphorus-boron-iron co-doped coconut shell activated carbon carrier provided by the invention is loaded with the metal catalyst, the common problems of low conversion rate and poor selectivity in nitro hydrogenation reaction can be solved.

This invention provides high-purity ferric citrate that is substantially free of β-iron hydroxide. [Solution] A high-purity ferric citrate that substantially does not contain β-iron hydroxide, and in which the β-iron hydroxide content is less than 6% by weight relative to its total weight. Preferably, high-purity ferric citrate having a β-iron hydroxide content of less than 2.5% by weight. More preferably, high-purity citric acid II having a β-iron oxide content of less than 1.0% by weight. iron.

To provide methods for treating a subject with chronic kidney disease.SOLUTION: The present invention discloses pharmaceutical-grade ferric organic compounds having enhanced dissolution rates. These ferric organic compounds, including but are not limited to ferric citrate, are useful for treating chronic kidney disease.SELECTED DRAWING: Figure 1

The invention provides a solid-state luminous magnetic carbon dot fluorescent material as well as a preparation method and application thereof. Ammonium ferric citrate is taken as an iron source, basic fuchsin is taken as a carbon source, polyethyleneimine is taken as a cross-linking agent, and magnetic Fe3O4 is formed through reaction of polyethyleneimine and ammonium ferric citrate; magnetic Fe3O4 and carbon dots formed by a carbon source are connected through dehydration condensation of amino of polyethyleneimine and carboxyl on the surface of basic fuchsin, the solid-state light-emitting magnetic carbon dot fluorescent material in which the carbon dots and the magnetic Fe3O4 are dispersed and are not in contact is obtained, AIQ does not occur in the aggregate, solid-state light emitting of the magnetic carbon dot fluorescent material can be achieved, and the magnetic carbon dot fluorescent material has good application prospects. The magnetic carbon dot fluorescent material can be applied to fingerprint extraction.

The application discloses a preparation method of cigar tobacco deep color jacket based on oxygen-increasing fermentation, and relates to the technical field of cigar production and processing. The method is characterized by the following steps: soaking the dried cigar tobacco in a mixed solution of ferric citrate and xylo-oligosaccharide, naturally air-drying, controlling the water content of the cigar tobacco raw material, and then performing fermentation treatment to obtain the finished product of the cigar tobacco deep color jacket. The method can effectively deepen the color of the cigar tobacco, meet the quality requirements of the jacket, and obviously improve the aroma quality and aroma amount of the jacket. When the oxygen concentration in the fermentation environment is controlled to be about 29% at the same time, the above-mentioned effects are more obvious, and the economic value of the cigar jacket product is significantly improved.

The invention discloses a preparation method of an anti-pulverization catalyst for synthesizing high-concentration COS, and the specific steps are as follows: Dissolve ammonium ferric citrate and calcium chloride in deionized water, add ethylene glycol and urea, stir and mix evenly, and let stand for 2 to 24 hours, then pour it into an ultrasonic atomizer for atomization treatment to form an atomized mixed solution; Introduce the atomized mixed solution into a high-temperature tubular furnace by pressurized carbon dioxide gas for heating and decomposition, and collect the sample powder generated by decomposition through electrostatic adsorption; Place the sample powder in a sugar coating machine, spray an atomized liquid containing a granulating agent for granulation to form spherical structures; Roast the spherical structures and then perform sulfidation treatment to obtain the required anti-pulverization catalyst for synthesizing high-concentration COS. The catalyst prepared by the invention has the characteristics of long service life, high mechanical strength, low loss rate, and strong anti-pulverization ability. When used in the dry method for preparing COS, the catalyst has a high conversion rate of CO and good selectivity for the product COS.

The invention belongs to the technical field of biology, and relates to a method for improving the expression quantity of HEK 293 cell transient transfection recombinant protein, which comprises the following steps: resuscitating and subculturing HEK 293 cells, adding the cells with adjusted density into a recombinant protein DNA-PEI compound to carry out first transient transfection, and carrying out second transient transfection to obtain the HEK 293 cell transient transfection recombinant protein. The HEK 293 cells are subjected to secondary transient transfection after the primary transient transfection, an additive is added into a transfection system after the secondary transient transfection, and the additive comprises ammonium ferric citrate and tryptone. According to the method, the expression level of the target protein is remarkably improved.

The invention relates to the technical field of agricultural environment restoration and plant nutrition regulation and control, in particular to a passivation culture solution for rice cadmium reduction and a preparation method of the passivation culture solution. The culture solution is composed of a ferric citrate complexing solution, a sodium polyaspartate graft, potassium dihydrogen phosphate, a choline-stabilized orthosilicic acid mother solution and a zinc ethylenediamine tetraacetate disodium salt hydrate. In the preparation process, a stable system is cooperatively constructed through multi-coordination complexing, electrostatic regulation and an organic framework stabilization mechanism. The culture solution still keeps good dispersity and stability under acidic and hard water conditions, can form a weak-acid-to-neutral buffer area in rice rhizosphere, effectively reduces migration and absorption of cadmium ions, maintains availability of nutrient elements such as iron and zinc, remarkably reduces the cadmium content of rice grains, improves the nutritional quality and environmental adaptability of crops, and has a wide application prospect. The method is suitable for efficient and safe treatment of cadmium-polluted rice fields.

In the ferric iron citrate monohydrate defined by the chemical formula FeC6H6O7 H2O (ferrous citrate monohydrate or ferric iron citrate monohydrate) obtained according to a specific production method, the citric acid and the elemental iron are combined in a molecular complex in which the iron is gathered in the citrate moving into a ring-like form and the iron is bound to the O groups of the carboxyls 1 and 3 through ionic bonds, forming two enantiomers D and L (R and S), both of which are completely water-soluble and highly absorbable by the human body. Formula (L) and Formula (D). With a different absorption mechanism than traditional iron molecules, said ferric iron citrate monohydrate can be used in pharmaceutical compositions and / or nutritional compositions and / or food compositions, in particular for the treatment and prevention of iron deficiency anemia and all diseases and conditions associated with iron deficiency. The effectiveness is demonstrated through several examples.

The invention relates to the technical field of chemical analysis and detection, and aims to provide a high-sensitivity and strong-specificity ferric ammonium citrate detection method aiming at the defect of determining the content of ferric ammonium citrate in a high-salt system so as to improve the detection precision of the content of ferric ammonium citrate in table salt. The K-g-C3N4 (at) PDA nano-enzyme is prepared by adopting a two-step sylvite-assisted thermal copolymerization strategy, the nano-enzyme has peroxidase-like activity and can catalytically oxidize a substrate ABTS and H2O2 to generate a blue-green oxidation product (ox-ABTS) and generate maximum absorption at the wavelength of 416 nm under an acidic condition, ammonium ferric citrate can enhance the peroxidase-like activity of the K-g-C3N4 (at) PDA nano-enzyme, and the K-g-C3N4 (at) PDA nano-enzyme can be used for preparing the nano-enzyme. The concentration of the ferric ammonium citrate in the sample can be measured according to the linear relationship between the concentration of the ferric ammonium citrate and the increase of the absorbance of the ox-ABTS, so that the green color of the ox-ABTS is deepened, and the concentration of the ferric ammonium citrate in the sample can be measured according to the linear relationship. The K-g-C3N4-coated PDA nano enzyme is good in stability and high in salt tolerance, and various ions in the table salt have almost no influence on the detection result, so that the K-g-C3N4-coated PDA nano enzyme has good selection specificity and wider applicability.

The invention provides a preparation method and application of ammonium ferric citrate. The preparation method of the ferric ammonium citrate comprises the following steps: carrying out a first reaction on an iron compound and first ammonia water, introducing air in the process of the first reaction, and preparing a first slurry, and carrying out solid-liquid separation on the first slurry, taking a solid, carrying out second reaction on the solid, second ammonia water and citric acid in a solvent, and concentrating to prepare the ferric ammonium citrate. According to the preparation process of the ferric ammonium citrate, the generation of hydrogen and the use of a strong oxidant in the traditional process are avoided, the safety of the production process is improved, the ferric ammonium citrate with few impurities and high purity is obtained, qualified diammonium hydrogen phosphate is co-produced, and the preparation process has a great application prospect.

The invention discloses a method for treating pollutants by directionally activating persulfate through a single-Fe-site carbon-based material, and belongs to the technical field of wastewater treatment. The preparation method comprises the following steps: treating shell powder by using an acid solution, then successively mixing with dicyandiamide, potassium salt and ferric citrate, and then carrying out annealing treatment to obtain the single-Fe-site carbon-based material. Monatomic Fe species are anchored on the surface of the nitrogen-doped carbon nano tube to form an Fe-N4 local coordination structure with a bent interface, peroxymonosulfate is activated by using a single Fe site carbon-based material to generate an adsorption state * OOOH active species, high specificity is achieved, the catalytic effect on wastewater is remarkable, reproducibility is high, a catalytic system is stable, large-scale popularization is facilitated, and the method is suitable for industrial production. Wide application prospects are realized.

The invention provides a dual-pore-forming iron-carbon composite filler for strengthening Feammox-NDFO autotrophic nitrogen removal and a preparation method thereof, and relates to the technical field of sewage organism autotrophic nitrogen removal, the dual-pore-forming iron-carbon composite filler comprises the following raw materials by weight: 30-45 parts of iron-containing powder, 15-30 parts of charcoal powder, 20-30 parts of an adhesive, and 10-15 parts of a pore-forming agent. The iron-containing powder is one or a combination of iron sesquioxide (Fe2O3), iron phosphate (FePO4), goethite (FeO (OH)) and ferric citrate; the pore-forming agent is sodium bicarbonate; the invention relates to a preparation method of a dual-pore-forming iron-carbon composite filler for strengthening Feammox-NDFO autotrophic nitrogen removal. The preparation method specifically comprises the following steps: 1, pretreating raw materials; 2, mixing and granulating; 3, steam curing and pore forming are carried out, wherein the curing temperature is 60-80 DEG C; the humidity is 80-90% RH; 4, ultrasonic-assisted deionized water soaking and pore forming are carried out, and the soaking temperature is 30-50 DEG C; the time is 6-12 hours; the ultrasonic frequency is 20-40 kHz. And 5, screening a finished product. The filler disclosed by the invention has the beneficial effects that the filler is mainly applied to a low-carbon sewage condition that C / N is less than or equal to 0.5, efficient denitrification can still be kept under the low-carbon condition, and the TN removal rate can reach 91.6-97.8%.

A method of preparing a FeMnCeOx biomaterial is provided, including the following steps. A Pseudomonas sp. strain KW-2 is obtained. A culture medium with a pH of 6.5-7.8 is prepared, which includes 0.1 g / L K2HPO4, 0.2 g / L MnSO4·7H2O, 0.2 g / L NaNO3, 0.1 g / L CaCl2), 0.1 g / L NH4Cl, 0.1 g / L (NH4)2CO3, 35 g / L NaCl and 150 mg / L ferric ammonium citrate. The culture medium is autoclaved, inoculated with the KW-2 strain, cultured for 1-3 days, added with a cerium nitrate solution, cultured for 3-7 days and centrifuged at 4,000-8,000 rpm for 10-20 min to collect a precipitate. The precipitate is rinsed 5-8 times with deionized water and 0.01 mol / L phosphate buffered saline (PBS) and freeze-dried at −60° C. to obtain the FeMnCeOx biomaterial. A method for treating antibiotic wastewater using the FeMnCeOx biomaterial is also provided.

The invention discloses a method for in-situ CO2 conversion and activation of Fe-K modified porous activated carbon and application of the Fe-K modified porous activated carbon. The method comprises the following steps: (1) dipping and drying: adding ferric citrate, potassium carbonate and a carbon material into water, fully dissolving and dispersing, heating at 50-90 DEG C to evaporate moisture to obtain a Fe-K loaded carbon material, and drying in a drying oven at 80-120 DEG C for later use; (2) high-temperature pyrolysis: carrying out high-temperature pyrolysis on the Fe-K loaded carbon material at 500-700 DEG C for 2-6 hours to obtain a Fe-K modified carbon material; and (3) blending pyrolysis: uniformly mixing the Fe-K modified carbon material and limestone in a mass ratio of (0.1-3): 1, carrying out high-temperature co-pyrolysis for 2-8 hours, and reacting at a constant temperature of 600-900 DEG C to obtain the Fe-K modified porous activated carbon. According to the method, carbon material activation and CO2 generated by limestone decomposition are linked, carbonate decomposition and charcoal activation are integrated, the carbon reaction activity is increased by introducing iron and potassium elements, in-situ reduction of CO2 generated in the carbonate decomposition process in the cement industry is achieved, and meanwhile the selectivity of in-situ CO2 conversion can be improved.

The invention discloses a simple and low-cost method for preparing nitrogen (iron)-doped three-dimensional porous graphene, which comprises the following steps: by taking typha angustifolia leaves as a carbon precursor and ferric ammonium citrate as a nitrogen source and a graphitization catalyst, carrying out microwave heating pore-forming, carbonization and graphitization to obtain the nitrogen (iron)-doped three-dimensional porous graphene. The nitrogen content, the iron content, the specific surface area and the graphite degree of the material can be controlled according to the use amount of the ammonium ferric citrate and the microwave time. The material is metal-free nitrogen-doped three-dimensional porous graphene after iron removal.

The invention relates to the technical field of microorganisms. The invention particularly discloses a culture medium and a preparation method and application thereof. Each liter of the culture medium comprises 3-8 g of L-asparagine, 0.3-1 g of buffer salt, 8-15 g of a carbon source, 50-100 mL of glycerin, 0.01-0.1 g of ferric ammonium citrate, 0.1-0.5 g of magnesium sulfate, 1-5 g of citric acid and the balance of water. Wherein the carbon source is selected from one or more of sodium pyruvate, glucose and glucose monohydrate; the buffer salt is selected from monopotassium phosphate and / or sodium dihydrogen phosphate. The culture medium is simple in component, wide in source and low in cost, and low-cost culture of mycobacteria can be achieved.