The invention discloses a gynecological disease detection preparation, and belongs to the technical field of gynecological diseases; and the gynecological disease detection preparation is used for detecting the gynecological disease. The gynecological disease detection preparation comprises first liquid, second liquid, third liquid and fourth liquid; the first liquid is composed of the following components including, by mass, 0.5%-1.5% of crystal violet, 0.3%-1.5% of ammonium oxalate, 5%-20% of ethyl alcohol, 2%-5% of polyethylene glycol, 1%-5% of Triton and/or Tween and the balance distilledwater; the second liquid is composed of the following components including, by mass, 0.8%-2.3% of iodine, 1%-4% of potassium iodide, 0.1%-0.5% of hydrochloric acid and the balance distilled water; thethird liquid is composed of the following components including, by mass, 15%-35% of acetone, 40%-70% of ethyl alcohol and 5%-25% of glutaraldehyde; and the fourth liquid is composed of the followingcomponents i including, by mass, 0.3%-0.8% of basic fuchsin, 0.1%-0.6% of phenol, 5%-15% of ethyl alcohol, 2%-8% of polyethylene glycol, 0.5%-3% of Triton and/or Tween and the balance distilled water.The detection preparation provided by the invention is easy to prepare, easy to store and not prone to sediment and going bad, and the storage time can be up to 2 years; the detection preparation provided by the invention can be compatible with manual dying or automatic dying of the instrument, and the manual dying can be completed within the relatively short time.