The invention discloses a manufacturing method of a permanent microscopic slide specimen for tiny insects. The manufacturing method comprises the steps of fixing, decolorizing, dehydrating, color fixing,
drying and sealing. The manufacturing method has the beneficial effects that firstly, compared with an existing specimen manufacturing method, the manufacturing method of the slide specimen is simple in step, the used materials cannot cause environmental
pollution, the
dyeing effect is good, and the complexity of the specimen manufacturing process and the harm to a
human body can be reduced; secondly, according to the manufacturing method of the slide specimen, specimen
drying is achieved through a constant-temperature
vacuum chamber, sufficient
evaporation of a solution in the specimen is facilitated, and the problems that internal bubbles appear due to existing natural
air drying and the surface of the specimen is turbid are solved; finally, according to the manufacturing method of the slide specimen,
insect bodies are dyed through an
acid fuchsin alcohol solution, the body structures of the
insect bodies can be more obviously shown, and shrinkage and deformation of the
insect body structures cannot be caused through layer-by-layer
dehydration of
alcohol with different concentrations.