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Collagenous fiber Masson trichrome stain kit, preparation method thereof and staining method

A technology for dyeing reagents and collagen fibers, which is applied in the field of collagen fiber Masson tricolor staining kit and its preparation, can solve the problems of complicated operation and high cost, and achieve the effect of saving dyeing reagents

Inactive Publication Date: 2018-04-13
苏州泽科生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a collagen fiber masson trichrome dyeing kit and its preparation method and dyeing method, in order to solve the problems of complex operation and high cost caused by relying on dyeing tank dyeing in the prior art

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  • Collagenous fiber Masson trichrome stain kit, preparation method thereof and staining method

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Embodiment Construction

[0031] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them. the embodiment.

[0032] The invention provides a collagen fiber masson three-color staining kit, which includes a box body, which includes 6 drop bottles, each of which is equipped with different reagents, and the capacity of the drop bottle is 5ml or 10ml. The reagents in the dropping bottle are hematoxylin staining solution, hydrochloric acid differentiation solution, bluing solution, Ponceau fuchsin solution, phosphomolybdic acid solution and aniline blue solution.

[0033] This embodiment provides a method for preparing a collagen fiber Masson trichrome staining kit, comprising the following steps:

[0034] 1) Reagent preparatio...

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Abstract

The invention relates to a collagenous fiber Masson trichrome stain kit, a preparation method thereof and a staining method. The stain kit comprises a kit body, wherein 6 dropping bottles are contained in the kit body; different reagents are respectively contained in the 6 dropping bottles; the volumes of the dropping bottles are 5ml or 10ml; the reagents in the 6 dropping bottles are hematoxylinstaining solution, hydrochloric acid differentiation solution, back-to-blue solution, ponceau acid fuchsin solution, phosphomolybdic acid solution and aniline blue solution separately. The kit performs optimization in terms of the packaging of the kit, formulas of reagents and a staining process, further a collagenous fiber Masson trichrome stain kit based on instillation is assembled, and the kitcan be directly applied to rapid staining of small-scale tissue slices, and has the characteristics of economical efficiency, practicability, special effect and speediness.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a masson trichrome dyeing kit for collagen fibers, a preparation method and a dyeing method thereof. Background technique [0002] With the rapid development of pathology, pathological tissue staining has become one of the key technologies in this field, especially the eosin-hematoxylin staining method based on the basic structure of the tissue, which can not only directly reflect the changes of the cytoplasm and nucleus inside the tissue, but also can be used by cells and Changes in the nucleus determine the pathological state of the tissue cells. However, due to the complexity of cell results, there are also a variety of special structures in the tissue content, such as collagen fibers, reticular fibers, elastic fibers, muscle tissue, fat, glycogen, mucus, pathological deposits (such as copper, hemosiderin, etc.) etc.), nucleic acid, etc., how to quickly define the changes of spec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/31
CPCG01N1/31G01N2001/302
Inventor 吴永红
Owner 苏州泽科生物科技有限公司
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