45 results about "Tissue staining" patented technology

The invention relates to a CACNA1A-based mental patient heart death intelligent identification system and method, the system comprises an immunohistochemical staining device, an image acquisition unit, an image analysis unit and an intelligent identification unit, the immunohistochemical staining device is used for performing CACNA1A immunohistochemical staining on formalin-fixed paraffin-embedded heart tissue slices; the image acquisition unit is used for shooting and obtaining a heart tissue CACNA1A immunohistochemical staining image; the image analysis unit is used for processing the heart tissue CACNA1A immunohistochemical staining image so as to determine a tissue staining region and image features thereof; and the intelligent identification unit outputs an identification prediction result of heart death of the mental patient according to the image features of the tissue staining region. Compared with the prior art, the mental patient heart death identification system can intelligently and accurately identify mental patient heart death.

A method of forming an imaging calibration device for a biological material imaging system is provided. A first set of one or more discrete regions is provided upon or within a retaining member. Each said region of the first set comprises a selected tissue stain material, wherein each of the selected tissue stain materials exhibits a predetermined optical response inside a colour-stable region of a chromaticity diagram defined by the ellipse x2 / a2+y2 / b2=1, where a=0.21, b=0.135, the rotation is −0.07 rad and the translation is (0.38,0.333). A second set of one or more discrete regions is also provided upon or within the retaining member. The second set is formed of one or more coloured filters having a predetermined optical response outside the colour-stable region.

The invention relates to the technical field of plant genetic engineering, and discloses a limonium bicolor gene LbYAB1 and application thereof. The new function of the LbYAB1 is found, firstly, the expression of the LbYAB1 in salt glands and leaf primordia is proved through RNA in-situ hybridization and a promoter driven GUS tissue staining technology, and the expression of the LbYAB1 is induced by treatment of 6-benzylaminopurine. Secondly, a genetic transformation technology is updated, a stable overexpressed LbYAB1 strain is obtained, and by exploring the LbYAB1 function of an overexpressed and silent strain, it is found that compared with a wild type, development of salt glands of the overexpressed strain is inhibited, the salt secreting capacity is weakened, and the salt tolerance is reduced; a silent strain shows an opposite phenotype, and a result proves that the LbYAB1 is a negative regulation factor for salt gland development. Overexpression of the gene in limonium bicolor and arabidopsis thaliana both leads to enhancement of salt sensitivity. The invention not only lays a foundation for further exploring and revealing the development mechanism of salt glands, but also opens up a new path for cultivating salt-tolerant crops.

The present application relates to a kind of biological tissue staining machines, including main component, moving mechanism and liquid suction dehumidification structure, main component includes shell, work tank, installation groove, mounting bracket and bearing frame, the top of shell is equipped with work tank, the bottom of work tank is equipped with installation groove. By setting up liquid suction dehumidification structure, can be actively connected the dyeing liquid, dehydrating agent dropped in dyeing process, avoid the formation of effusion in the bottom of work tank, reduce reagent waste from the source;Prevent the abnormal humidity in tank caused by effusion volatilization simultaneously, guarantee the reagent concentration stability of subsequent dyeing step, and avoid effusion corrosion equipment components, pollute the tissue to be dyed, significantly improve the accuracy of dyeing result.

A system including: a carousel including a plurality of mounting stations dimensioned to receive at least one fluid dispensing cartridge; and a receiving assembly positioned beneath the carousel, the receiving assembly including a plurality of reaction stations, each of the plurality of reaction stations including: a body comprising a length dimension and a width dimension that together define a chamber to accommodate a single slide therein; and a lid comprising a first position to cover the chamber and a second position to expose a portion of the chamber. A method including moving a lid of a reaction station to expose a single microscope slide including a sample and dispensing one or more reagents on the sample.

The application discloses a method for generating protein expression information based on tissue staining images, comprising: inputting a tissue staining image to be analyzed into a trained neural network model, and generating protein expression information of at least one protein channel through inference of the neural network model, wherein the neural network model is trained in the following manner: cross-modal feature alignment training is performed on a tissue feature coding network in a neural network model to be trained, so that tissue structure image features are aligned with protein expression image features, to obtain a trained tissue feature coding network; the trained tissue feature coding network and a decoding network in the neural network model to be trained are taken as the neural network model to be trained, and protein expression supervised training is performed, to obtain the trained neural network model. The application reduces complex experimental steps and equipment requirements, can significantly reduce experimental costs and improve the efficiency of obtaining protein expression information.

A sample processor including a body including an inner surface that defines a chamber including an opening in a side of the body and including a volume to accommodate a least one microscope slide therein; a door comprising a first position to cover the opening of the body and a second position to expose a portion of the chamber through the opening; a slide bed disposed in the chamber; one of an internal humidity generator disposed in the chamber and an external humidity generator coupled to the chamber. A system including at least one sample processor at least one reagent outside the body of the at least one sample processor and a method including subjecting a sample on a microscope slide in a sealed chamber to a pressure greater than ambient and a humidity greater than 60 percent; and processing the sample. 20 25 20 31 55 02 M ay 2 02 5 A B S T R A C T 2 0 2 5 2 0 3 1 5 5 0 2 2 0 2 5 M a y

The invention belongs to the technical field of biological medicine, and provides a human umbilical cord mesenchymal stem cell preparation and application thereof, the preparation comprises human umbilical cord mesenchymal stem cells, ginsenoside Rg3 and a medicinal carrier; the medicinal carrier is a phosphate buffer solution; according to the application, 1 * 10 < 6 > HUCMSCs pretreated by Rg3 are subjected to caudal vein injection on a T2DM mouse model induced by high fat diet combined with STZ, the curative effect and mechanism are verified by combining OGTT, IPITT, serum biochemical detection, HE / PAS tissue staining, q-PCR and other technologies, and finally the purposes of improving glycometabolism of the T2DM mouse, repairing liver / kidney / pancreas target organ injuries, protecting pancreas islet beta cell functions, reducing pancreas islet beta cell functions, reducing pancreas islet beta cell functions, reducing pancreas islet beta cell functions, reducing pancreas islet beta cell functions and the like are achieved. And a theoretical basis is provided for accurate intervention of T2DM by activating an IGF-1R / PI3K-AKT signal channel and regulating and controlling the expression of a glycometabolism-related gene.

The present invention provides for water-soluble mono- and dicationic fluorescent dyes with the latter exhibiting stable fluorescence at elevated temperatures. The present invention also provides for methods for the production of the fluorescent dyes and for using these dyes in biological assays such as multiplexing qPCR and tissue staining.

A system including: a carousel including a plurality of mounting stations dimensioned to receive at least one fluid dispensing cartridge; and a receiving assembly positioned beneath the carousel, the receiving assembly including a plurality of reaction stations, each of the plurality of reaction stations including: a body comprising a length dimension and a width dimension that together define a chamber to accommodate a single slide therein; and a lid comprising a first position to cover the chamber and a second position to expose a portion of the chamber. A method including moving a lid of a reaction station to expose a single microscope slide including a sample and dispensing one or more reagents on the sample.

Disclosed herein is a method of analysing a tissue sample. The method comprises identifying one or more regions of interest within the tissue sample based on a tissue stain that has been applied to the tissue sample. Analyte material is then generated from the one or more regions of interest identified based on the tissue stain using a direct surface sampling probe (10), which analyte material is then received at a sampling inlet (30) and passed towards a mass and / or ion mobility spectrometer (50) for analysis.

A system comprising: a turntable including a plurality of mounting stations sized to receive at least one fluid dispensing cartridge; and a receiving assembly positioned below the turntable, the receiving assembly including a plurality of reaction stations, each of the plurality of reaction stations including: a body including length and width dimensions of a chamber together defining a cavity for receiving a single slide; and a cover including a first position covering the cavity and a second position exposing a portion of the cavity. A method comprising moving the cover of a reaction station to expose a single microscope slide containing a sample, and dispensing one or more reagents onto the sample.

A system including: a carousel including a plurality of mounting stations dimensioned to receive at least one fluid dispensing cartridge; and a receiving assembly positioned beneath the carousel, the receiving assembly including a plurality of reaction stations, each of the plurality of reaction stations including: a body comprising a length dimension and a width dimension that together define a chamber to accommodate a single slide therein; and a lid comprising a first position to cover the chamber and a second position to expose a portion of the chamber. A method including moving a lid of a reaction station to expose a single microscope slide including a sample and dispensing one or more reagents on the sample. 20 25 23 41 61 17 S ep 2 02 5 2 0 2 5 2 3 4 1 6 1 1 7 S e p 2 0 2 5 A B S T R A C T

To provide a biological tissue staining reagent, a biological tissue staining kit, and a biological tissue staining method in which an antibody can be sufficiently impregnated into a tissue.SOLUTION: A biological tissue staining reagent includes a peptide having a second amino acid sequence at least four amino acid of which matches with a first amino acid sequence of epitope in an antigen in a biological tissue being a target of immunostaining by an antibody.SELECTED DRAWING: Figure 5

A sample processor including a body including an inner surface that defines a chamber including an opening in a side of the body and including a volume to accommodate a least one microscope slide therein; a door comprising a first position to cover the opening of the body and a second position to expose a portion of the chamber through the opening; a slide bed disposed in the chamber; one of an internal humidity generator disposed in the chamber and an external humidity generator coupled to the chamber. A system including at least one sample processor at least one reagent outside the body of the at least one sample processor and a method including subjecting a sample on a microscope slide in a sealed chamber to a pressure greater than ambient and a humidity greater than 60 percent; and processing the sample.

The application provides a tissue staining method based on antigen repair treatment combined with silver staining, comprising the following steps: a) high-temperature heat repair of a tissue section to be treated with an antigen repair solution, and washing with water after cooling to obtain a repaired tissue section; b) silver staining of the repaired tissue section obtained in step a), dehydration, and then mounting to obtain a stained tissue section. Compared with the prior art, the tissue staining method based on antigen repair treatment combined with silver staining provided by the application adopts specific process steps and conditions to realize overall good interaction: first, the brain tissue is subjected to antigen repair treatment, and then the silver staining technology is adopted, which significantly improves the signal-to-noise ratio of the senile plaque region, and ideal staining effect is obtained, which can effectively improve the detail visibility in pathological detection, help more accurately evaluate the morphology and distribution characteristics of the senile plaque, and has a wide application prospect.

The application belongs to the technical field of pathological tissue processing, and particularly relates to a pathological tissue staining automatic control method and a staining machine system. The method provided by the application detects and calculates the staining quality after each staining, adjusts the staining processing time of hematoxylin and eosin according to the staining quality, and replaces the staining solution if the staining processing time of hematoxylin and eosin exceeds the preset maximum value. The method provided by the application can effectively prolong the service life of the staining reagent, and can also ensure the uniformity and stability of pathological section staining.

This invention relates to the field of soft tissue biomechanical modeling technology, specifically to a constitutive modeling method suitable for J-type soft tissue. The method includes: processing the soft tissue using tissue staining to obtain the volume fractions of matrix, collagen fibers, and elastic fibers; determining an isotropic matrix term based on the characteristics of mesenchymal cells and endothelial cells in the matrix; determining a collagen fiber term based on the characteristics of collagen fiber curling, straightening, fiber rotation, and interfiber slippage; determining an elastic fiber term based on the fiber rotation characteristics of elastic fibers, thus obtaining a model of undetermined parameters; adding the volume fractions of matrix, collagen fibers, and elastic fibers in the soft tissue to the model of undetermined parameters; and using a nearest-nearest global optimization differential evolution algorithm to solve for the undetermined parameters in the model of undetermined parameters, thereby obtaining a hyperelastic constitutive model. This invention can improve the accuracy of the biomechanical property model of J-type soft tissue.

An autonomous quality and hallucination assessment method (AQuA) is disclosed, designed for either virtual tissue staining or histochemical staining. AQuA uses a trained neural network to autonomously achieve 99.8% accuracy when detecting acceptable and unacceptable virtually stained tissue images without access to ground truth, also presenting an agreement of 98.5% with the manual assessments made by a group of board-certified pathologists. AQuA achieves super-human performance in identifying realistic-looking, virtually stained hallucinatory images that would normally mislead human diagnosticians. The wide adaptability of AQuA is demonstrated across various virtually and histochemically stained human tissue images and showcase its strong external generalization to detect unseen hallucination patterns of virtual staining network models as well as artifacts observed in the traditional histochemical staining workflow.

The invention provides application of auramine O in neuronal death and DNA damage detection and a detection method. Auramine O is an ultrafast fluorescent rotor which is rarely applied to tissue staining, can be specifically combined with an endangered or dead neuron, and shows a spatial and temporal distribution characteristic consistent with Fluro-Jade B staining. The technology is extremely sensitive to desoxyribonuclease pretreatment, which indicates that the binding site of auramine O is mainly located in the damaged neuron DNA molecule, so that auramine O not only can detect neuron death, but also can reflect the damage condition of neuron DNA. According to the technical scheme, operation is fast and simple, and the influence of a sample fixing mode is small; the method can be independently applied to detection of neuron death and can also be combined with other fluorescence detection technologies for application. According to the technology, damaged neurons are disclosed, neuron death detection and neuron death mechanism research are combined together, and an effective means is provided for neuroscience research.

The invention relates to the technical field of immunofluorescent staining, in particular to an adipose tissue whole-tissue immunofluorescent staining method based on tabletting treatment. According to the method, tissue acquisition and fixation, tissue tabletting, membrane rupture and sealing, degreasing and autofluorescence removal, fluorescent staining, sheet sealing and imaging are sequentially carried out. According to the invention, a complete tabletting-degreasing-fluorescent staining integrated process is established, a set of systematic process which is standardized, high in repeatability and adaptive to conventional experimental conditions is provided, and tabletting whole tissue staining is realized in adipose tissues for the first time. The core of the invention lies in that the thickness of the tissue is reduced by a tabletting treatment means, and a set of optimized antibody permeation, sealing, degreasing and anti-autofluorescence treatment flow is matched, so that the immunofluorescent staining of the adipose tissue is effectively realized on the premise of not depending on complex tissue transparency and special imaging equipment, and the immunofluorescent staining effect is greatly improved. The method is especially suitable for detecting deep or low-abundance targets such as nerve markers.

The invention relates to a method for obtaining pathological diagnosis according to a color ultrasound image of a thyroid nodule by applying an artificial intelligence technology. The thyroid nodule color Doppler ultrasound image-pathological diagnosis system has the functions of image acquisition, image preprocessing, feature extraction, image recognition and the like, and can recognize, extract and memorize the color Doppler ultrasound image features of thyroid nodules and the internal features of abnormal cells in pathological tissue staining pictures of the thyroid nodules. Color Doppler ultrasound image data of thyroid nodules directly correspond to pathological picture data, and the data can be interpreted. A color Doppler ultrasound image database and a pathological picture database of thyroid nodules are established, large-scale machine learning is carried out with sufficient data, and functions of the machine learning are improved. The system is verified and clinically tested to ensure the accuracy of the system, and pathological diagnosis is displayed. The method has the advantages that the pathological diagnosis can be obtained when nodules are seen on the thyroid color ultrasound, and the purpose of accurate treatment can be achieved. Finally, clinical diagnosis is based on pathological results under a microscope.

This invention discloses the application of potassium iodide in reducing the fruit drop rate of pecans, belonging to the field of plant growth regulation technology. It provides a method for reducing the fruit drop rate of pecans using the aforementioned potassium iodide, comprising the following steps: applying potassium iodide solution to the junction of the pecan fruit and branch at the early stage of fruit development, 2-3 times, with an interval of 7-10 days between each application. This invention proposes the application of potassium iodide in reducing the fruit drop rate of pecans. Through statistical analysis, pecan morphology and anatomy, and tissue staining, the role of potassium iodide in controlling fruit drop was analyzed, demonstrating its application in reducing the fruit drop rate of pecans and slowing down the occurrence of fruit drop in Zhejiang pecans.

The invention discloses an in-vitro biological tissue staining buffer solution based on electric field-chemical closed loop pH dynamic regulation and control and application thereof. The dyeing buffer solution is mainly formed by dissolving the following components in deionized water: a buffer agent: a combination of one or two of tris (hydroxymethyl) aminomethane and 3-(cyclohexylamine)-1-propanesulfonic acid; the surfactant comprises the following components: TritonX-100 and Tween 20; the pH regulator is glucose; the in-vitro biological tissue staining buffer solution is arranged in direct-current electric field equipment. The reagent provided by the invention has simple components, is generally suitable for various dyeing molecules such as antibodies, fluorescent dyes and the like, and provides an efficient, mild and controllable electric field dyeing technical scheme for high-flux three-dimensional pathology, full-organ atlas drawing and accurate diagnosis.

Staining compositions and methods to stain a tissue are disclosed herein. The staining composition includes a diluent and a colorant comprising pigment particles having a D50 particle size of about 5 microns or less and a liquid carrier. The composition has a viscosity of about 10 Krebs Units or less. Using the staining composition, tissue may be stained directly at the point of excision from the patient in a minimum amount of time so that the specimen is identifiable and distinguishable at every point of risk from collection to analysis and reporting.

The invention discloses a micro-plastic organism in-vivo in-situ integrated analysis method based on phthalocyanine blue labeling and Raman imaging, and belongs to environmental toxicology and analytical chemistry technologies. The method comprises the following steps: firstly, exposing organisms to be researched and analyzed in phthalein blue dyed micro-plastic particles; then obtaining exposed biological tissues, preparing paraffin sections and staining the tissues; positioning a biological tissue area under an optical microscope, acquiring a spectrum of the area by adopting a Raman spectrum point scanning mode, and confirming a target area by identifying characteristic Raman peaks of phthalocyanine blue and micro-plastic; raman surface scanning is carried out on a target area, intensity information of characteristic Raman peaks of phthalocyanine blue or micro-plastic is obtained through a peak clamping method, a spatial distribution image of corresponding components in biological tissue is generated, and quantification of the micro-plastic can be achieved. According to the method, the problem that the marking stability of a traditional plastic dyeing method is difficult to be compatible with a standard slide making process is solved, in-situ association of micro-plastic distribution and tissue lesion can be realized, and the method is suitable for accurate detection of submicron micro-plastics.

The application discloses a kind of tissue transparentization compositions and preparation method thereof, wherein composition includes: transparentization medium, basic neutralizing agent, aqueous solvent, amphiphilic transition solvent and refractive index regulator, the transparentization medium is any one or several of surifenone, surifenone salt, p-xylylene dicyclohexanesulfonic acid and its salt or 2-phenyl benzimidazole-5-sulfonic acid and its salt, basic neutralizing agent is used to neutralize acidic group in transparentization medium to form clear homogeneous system;Refractive index regulator is used to adjust the refractive index of composition to the range matched with the target refractive index of the tissue to be treated.The composition of the application can realize the rapid transparentization of living tissue, and after flushing with PBS buffer, the transparentization effect can realize a certain degree of reversal, and the whole process does not cause tissue staining, which is beneficial to subsequent imaging analysis.

The biological tissue dyeing machine comprises a main body assembly, a moving mechanism and a liquid absorbing and dehumidifying structure, the main body assembly comprises a shell, a working groove, a mounting groove, a mounting frame and a bearing frame, the working groove is formed in the top of the shell, and the mounting groove is formed in the bottom of the working groove. By arranging the liquid absorbing and dehumidifying structure, staining liquid and a dehydrating agent dripping in the staining process can be actively received, liquid is prevented from forming accumulated liquid at the bottom of the working tank, and reagent waste is reduced from the source; meanwhile, abnormal humidity in the tank caused by accumulated liquid volatilization is prevented, the stability of the reagent concentration in the subsequent dyeing step is guaranteed, the accumulated liquid is prevented from corroding equipment parts and polluting to-be-dyed tissues, and the accuracy of the dyeing result is remarkably improved.

The utility model discloses a tissue staining jar, which belongs to the technical field of medical auxiliary instruments and comprises a staining jar, and a detachable jar cover is arranged at the top of the staining jar. The dyeing device comprises a dyeing cylinder, a separation frame body is arranged in the dyeing cylinder, the separation frame body is in sliding fit with the inner wall of the dyeing cylinder, a plurality of dyeing cavities are formed in the separation frame body, a separation plate is arranged in each dyeing cavity, and the separation plates are in inserted fit with the dyeing cavities. According to the utility model, the separation frame body can be detached from the staining jar, the separation plate can be detached from the staining cavity, and the staining jar, the separation frame body and the separation plate can be cleaned and disinfected after the staining of a glass slide is completed, so that cross contamination and impurity interference removal are avoided.

The waste gas treatment device comprises a pathological tissue dyeing machine body, a shell is arranged on the left side of the pathological tissue dyeing machine body, an air outlet and an air inlet are formed in the left side face and the right side face of the shell respectively, and the air inlet is communicated with the interior of the pathological tissue dyeing machine body. A primary filtering cavity, a chemical filtering cavity and a light filtering cavity which are communicated with one another are sequentially formed in the shell from right to left, and a primary filtering assembly, a chemical filtering assembly and a light filtering assembly are arranged in the primary filtering cavity, the chemical filtering cavity and the light filtering cavity respectively; the primary filtering cavity and the light filtering cavity are respectively communicated with the air inlet and the air outlet, and the air inlet and the air outlet are respectively provided with an air inlet fan and an exhaust fan; the multi-stage filter has the advantage of multi-stage filtration.