83 results about "Tissue staining" patented technology

A deep learning-based virtual HER2 IHC staining method uses a conditional generative adversarial network that is trained to rapidly transform autofluorescence microscopic images of unlabeled / label-free breast tissue sections into bright-field equivalent microscopic images, matching the standard HER2 IHC staining that is chemically performed on the same tissue sections. The efficacy of this staining framework was demonstrated by quantitative analysis of blindly graded HER2 scores of virtually stained and immunohistochemically stained HER2 whole slide images (WSIs). A second quantitative blinded study revealed that the virtually stained HER2 images exhibit a comparable staining quality in the level of nuclear detail, membrane clearness, and absence of staining artifacts with respect to their immunohistochemically stained counterparts. This virtual staining framework bypasses the costly, laborious, and time-consuming IHC staining procedures in laboratory, and can be extended to other types of biomarkers to accelerate the IHC tissue staining and biomedical workflow.

The invention relates to a CACNA1A-based mental patient heart death intelligent identification system and method, the system comprises an immunohistochemical staining device, an image acquisition unit, an image analysis unit and an intelligent identification unit, the immunohistochemical staining device is used for performing CACNA1A immunohistochemical staining on formalin-fixed paraffin-embedded heart tissue slices; the image acquisition unit is used for shooting and obtaining a heart tissue CACNA1A immunohistochemical staining image; the image analysis unit is used for processing the heart tissue CACNA1A immunohistochemical staining image so as to determine a tissue staining region and image features thereof; and the intelligent identification unit outputs an identification prediction result of heart death of the mental patient according to the image features of the tissue staining region. Compared with the prior art, the mental patient heart death identification system can intelligently and accurately identify mental patient heart death.

The invention belongs to the field of medicine application, and particularly discloses application of sarcosine serving as a unique active component to preparation of medicine for preventing and treating obesity and / or hyperlipidemia. The test of treating old mice with sarcosine proves that the sarcosine can reduce the total body mass and fat mass and reduce the body fat rate under the condition that the absolute lean body mass is not influenced. Through tissue staining and biochemical analysis, it is determined that sarcosine can reduce the cross sectional area of visceral adipose tissue (VAT) cells, up-regulate expression of a marker UCP1 of brown adipose tissue in visceral adipose tissue and remarkably reduce the level of triglyceride in plasma. The sarcosine has practical application value in treatment of obesity and hyperlipidemia.

The present invention relates to the technical field of biology, and in particular to a method for improving the H&E-stained image quality of a spatio-temporal chip, a reagent combination, a kit, and a device. The present invention provides a new mounting medium, which uses a simple reagent and corresponding process design, achieving the same effects of mRNA in-situ capture and comprehensive enhancement of mRNA diffusion as a glycerol mounting medium, and effectively solving the problems of fading and color diffusion after tissue staining. Registration of an H&E image and a spatio-temporal expression matrix of a BGI Stereo-seq chip provides possibility for high-precision subcellular-level omics analysis.

The invention relates to the technical field of optics, and discloses a visualization method of a biological tissue three-dimensional full-information atlas, which comprises the following steps of: acquiring image data, electron microscope data, mechanical data, protein distribution, omics information and histological staining images of biological tissues by using various biological visualization technologies, and correspondingly processing the acquired data to obtain a three-dimensional full-information atlas of the biological tissues; finally, organization information fusion and digital display are achieved. According to the method, a cross-scale feature correlation algorithm framework is innovatively established, and multi-source heterogeneous data fusion is performed on medical image topological data (CT / MRI), ultrastructure scanning data (SEM / TEM), molecular positioning information (immunofluorescence, immunohistochemistry and enzyme immunoassay), omics maps (space transcriptome / proteome) and high-resolution tissue staining data. The data island dilemma caused by traditional single-mode analysis is broken through, and full-scale visualization from nanoscale molecular distribution to centimeter-level organ tissue structure-function relationship is realized for the first time.

A method of forming an imaging calibration device for a biological material imaging system is provided. A first set of one or more discrete regions is provided upon or within a retaining member. Each said region of the first set comprises a selected tissue stain material, wherein each of the selected tissue stain materials exhibits a predetermined optical response inside a colour-stable region of a chromaticity diagram defined by the ellipse x2 / a2+y2 / b2=1, where a=0.21, b=0.135, the rotation is −0.07 rad and the translation is (0.38,0.333). A second set of one or more discrete regions is also provided upon or within the retaining member. The second set is formed of one or more coloured filters having a predetermined optical response outside the colour-stable region.

The invention relates to the technical field of plant genetic engineering, and discloses a limonium bicolor gene LbYAB1 and application thereof. The new function of the LbYAB1 is found, firstly, the expression of the LbYAB1 in salt glands and leaf primordia is proved through RNA in-situ hybridization and a promoter driven GUS tissue staining technology, and the expression of the LbYAB1 is induced by treatment of 6-benzylaminopurine. Secondly, a genetic transformation technology is updated, a stable overexpressed LbYAB1 strain is obtained, and by exploring the LbYAB1 function of an overexpressed and silent strain, it is found that compared with a wild type, development of salt glands of the overexpressed strain is inhibited, the salt secreting capacity is weakened, and the salt tolerance is reduced; a silent strain shows an opposite phenotype, and a result proves that the LbYAB1 is a negative regulation factor for salt gland development. Overexpression of the gene in limonium bicolor and arabidopsis thaliana both leads to enhancement of salt sensitivity. The invention not only lays a foundation for further exploring and revealing the development mechanism of salt glands, but also opens up a new path for cultivating salt-tolerant crops.

The present application relates to a kind of biological tissue staining machines, including main component, moving mechanism and liquid suction dehumidification structure, main component includes shell, work tank, installation groove, mounting bracket and bearing frame, the top of shell is equipped with work tank, the bottom of work tank is equipped with installation groove. By setting up liquid suction dehumidification structure, can be actively connected the dyeing liquid, dehydrating agent dropped in dyeing process, avoid the formation of effusion in the bottom of work tank, reduce reagent waste from the source;Prevent the abnormal humidity in tank caused by effusion volatilization simultaneously, guarantee the reagent concentration stability of subsequent dyeing step, and avoid effusion corrosion equipment components, pollute the tissue to be dyed, significantly improve the accuracy of dyeing result.

The present invention relates to the field of biological data processing, and discloses a lung cancer immune marker analysis method, device, electronic device and storage medium, wherein the method comprises: using fluorescent signal amplification technology to perform a multi-biomarker staining operation on lung tumor tissue to obtain a tissue staining image set; performing real cell selection on the tissue staining image set according to the region of interest input by the user to obtain a circled image set; performing quantitative statistics on each biomarker in the circled image set according to a preset optimal positive threshold screening strategy to obtain the tissue marker positive rate, interstitial marker positive rate and double positive rate between target biomarkers corresponding to each biomarker; and outputting the tissue marker positive rate, interstitial marker positive rate and double positive rate in a structured manner to obtain lung immune marker positive data. The main purpose of the present invention is to improve the comprehensiveness and accuracy of biomarker analysis of the lung cancer immune microenvironment.

A system including: a carousel including a plurality of mounting stations dimensioned to receive at least one fluid dispensing cartridge; and a receiving assembly positioned beneath the carousel, the receiving assembly including a plurality of reaction stations, each of the plurality of reaction stations including: a body comprising a length dimension and a width dimension that together define a chamber to accommodate a single slide therein; and a lid comprising a first position to cover the chamber and a second position to expose a portion of the chamber. A method including moving a lid of a reaction station to expose a single microscope slide including a sample and dispensing one or more reagents on the sample.

1. The name of the product under this design: Dyeing machine (fully automatic). 2. Purpose of the product of this design: used for pathological tissue staining. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture. 5. The bottom surface of this design product is a part that is not easily visible or cannot be seen when in use, so the top view is omitted.

The application discloses a method for generating protein expression information based on tissue staining images, comprising: inputting a tissue staining image to be analyzed into a trained neural network model, and generating protein expression information of at least one protein channel through inference of the neural network model, wherein the neural network model is trained in the following manner: cross-modal feature alignment training is performed on a tissue feature coding network in a neural network model to be trained, so that tissue structure image features are aligned with protein expression image features, to obtain a trained tissue feature coding network; the trained tissue feature coding network and a decoding network in the neural network model to be trained are taken as the neural network model to be trained, and protein expression supervised training is performed, to obtain the trained neural network model. The application reduces complex experimental steps and equipment requirements, can significantly reduce experimental costs and improve the efficiency of obtaining protein expression information.

A sample processor including a body including an inner surface that defines a chamber including an opening in a side of the body and including a volume to accommodate a least one microscope slide therein; a door comprising a first position to cover the opening of the body and a second position to expose a portion of the chamber through the opening; a slide bed disposed in the chamber; one of an internal humidity generator disposed in the chamber and an external humidity generator coupled to the chamber. A system including at least one sample processor at least one reagent outside the body of the at least one sample processor and a method including subjecting a sample on a microscope slide in a sealed chamber to a pressure greater than ambient and a humidity greater than 60 percent; and processing the sample. 20 25 20 31 55 02 M ay 2 02 5 A B S T R A C T 2 0 2 5 2 0 3 1 5 5 0 2 2 0 2 5 M a y

The invention belongs to the technical field of biological medicine, and provides a human umbilical cord mesenchymal stem cell preparation and application thereof, the preparation comprises human umbilical cord mesenchymal stem cells, ginsenoside Rg3 and a medicinal carrier; the medicinal carrier is a phosphate buffer solution; according to the application, 1 * 10 < 6 > HUCMSCs pretreated by Rg3 are subjected to caudal vein injection on a T2DM mouse model induced by high fat diet combined with STZ, the curative effect and mechanism are verified by combining OGTT, IPITT, serum biochemical detection, HE / PAS tissue staining, q-PCR and other technologies, and finally the purposes of improving glycometabolism of the T2DM mouse, repairing liver / kidney / pancreas target organ injuries, protecting pancreas islet beta cell functions, reducing pancreas islet beta cell functions, reducing pancreas islet beta cell functions, reducing pancreas islet beta cell functions, reducing pancreas islet beta cell functions and the like are achieved. And a theoretical basis is provided for accurate intervention of T2DM by activating an IGF-1R / PI3K-AKT signal channel and regulating and controlling the expression of a glycometabolism-related gene.

The present invention provides for water-soluble mono- and dicationic fluorescent dyes with the latter exhibiting stable fluorescence at elevated temperatures. The present invention also provides for methods for the production of the fluorescent dyes and for using these dyes in biological assays such as multiplexing qPCR and tissue staining.

The utility model discloses tissue immunofluorescence bleaching and dyeing equipment, which relates to the technical field of immunofluorescence and comprises a bottom plate, a cover plate is arranged on the upper side of the bottom plate, a groove is arranged on the upper side surface of the cover plate, the bottom end of the cover plate penetrates into the groove and is provided with a drain hole, and a water blocking plug is arranged on the upper side surface of the bottom plate and penetrates through the drain hole. According to the utility model, waste of tissues during slicing is avoided, a plurality of samples can be treated at the same time, the tissues can be dyed more fully and thoroughly, and non-specific dyeing can be reduced.

A system including: a carousel including a plurality of mounting stations dimensioned to receive at least one fluid dispensing cartridge; and a receiving assembly positioned beneath the carousel, the receiving assembly including a plurality of reaction stations, each of the plurality of reaction stations including: a body comprising a length dimension and a width dimension that together define a chamber to accommodate a single slide therein; and a lid comprising a first position to cover the chamber and a second position to expose a portion of the chamber. A method including moving a lid of a reaction station to expose a single microscope slide including a sample and dispensing one or more reagents on the sample.

A deep learning-based virtual HER2 IHC staining method uses a conditional generative adversarial network trained to rapidly convert autofluorescence microscopy images of unlabeled / unlabeled breast tissue sections into brightfield equivalent microscopy images that match standard HER2 IHC staining performed chemically on the same tissue sections. The effectiveness of the staining framework was demonstrated by a quantitative analysis of blindly graded HER2 scores of virtual stained HER2 whole slide images (WSIs) and immunohistochemically stained HER2 whole slide images. Another quantitative blinded study revealed that the virtual stained HER2 images exhibited comparable staining quality in levels of nuclear detail, membrane clarity, and absence of staining artifacts to their immunohistochemically stained counterparts. This virtual staining framework can avoid costly, laborious, and time-consuming IHC staining procedures in laboratories and can be extended to other types of biomarkers to facilitate IHC tissue staining and biomedical workflows.

Disclosed herein is a method of analysing a tissue sample. The method comprises identifying one or more regions of interest within the tissue sample based on a tissue stain that has been applied to the tissue sample. Analyte material is then generated from the one or more regions of interest identified based on the tissue stain using a direct surface sampling probe (10), which analyte material is then received at a sampling inlet (30) and passed towards a mass and / or ion mobility spectrometer (50) for analysis.

A system comprising: a turntable including a plurality of mounting stations sized to receive at least one fluid dispensing cartridge; and a receiving assembly positioned below the turntable, the receiving assembly including a plurality of reaction stations, each of the plurality of reaction stations including: a body including length and width dimensions of a chamber together defining a cavity for receiving a single slide; and a cover including a first position covering the cavity and a second position exposing a portion of the cavity. A method comprising moving the cover of a reaction station to expose a single microscope slide containing a sample, and dispensing one or more reagents onto the sample.

A tissue staining device (100) comprises: (a) a microscope slide carousel (105), wherein the microscope slide carousel (105) is configured to rotate on a first axis of rotation (230), and wherein the microscope slide carousel (105) comprises at least one microscope slide cartridge stall (106), and (b) a reagent assembly (110) comprising: (i) a reagent carousel (113), wherein the reagent carousel (113) is configured to rotate on a second axis of rotation (310), and wherein the reagent carousel (113) comprises at least one reagent holder, (ii) a dispensing actuator (115), and wherein the reagent carousel (113) is positioned above the microscope slide carousel (105), and wherein the reagent carousel (113) and the microscope slide carousel (105) are positioned such that when a reagent is placed in the reagent carousel (113) the reagent can flow from the reagent carousel (113) to the microscope slide carousel (105).

A system including: a carousel including a plurality of mounting stations dimensioned to receive at least one fluid dispensing cartridge; and a receiving assembly positioned beneath the carousel, the receiving assembly including a plurality of reaction stations, each of the plurality of reaction stations including: a body comprising a length dimension and a width dimension that together define a chamber to accommodate a single slide therein; and a lid comprising a first position to cover the chamber and a second position to expose a portion of the chamber. A method including moving a lid of a reaction station to expose a single microscope slide including a sample and dispensing one or more reagents on the sample. 20 25 23 41 61 17 S ep 2 02 5 2 0 2 5 2 3 4 1 6 1 1 7 S e p 2 0 2 5 A B S T R A C T

The invention provides a tissue staining method based on combination of antigen repair treatment and silver staining, which comprises the following steps: a) performing high-temperature thermal repair on a to-be-treated tissue slice by using an antigen repair liquid, cooling, and cleaning with water to obtain a repaired tissue slice; and b) performing silver staining on the repaired tissue slice obtained in the step a), dehydrating and sealing the slice to obtain the stained tissue slice. Compared with the prior art, the tissue staining method based on combination of antigen repair treatment and silver staining provided by the invention adopts specific process steps and conditions to realize relatively good overall interaction: firstly, the brain tissue is subjected to antigen repair treatment, and then the silver staining technology is adopted, so that the signal-to-noise ratio of an age pigment region is remarkably improved; compared with the prior art, an ideal staining effect is obtained, detail visibility in pathological detection can be effectively improved, the method helps to more accurately evaluate the morphology and distribution characteristics of the age pigment, and the method has a wide application prospect.

To provide a biological tissue staining reagent, a biological tissue staining kit, and a biological tissue staining method in which an antibody can be sufficiently impregnated into a tissue.SOLUTION: A biological tissue staining reagent includes a peptide having a second amino acid sequence at least four amino acid of which matches with a first amino acid sequence of epitope in an antigen in a biological tissue being a target of immunostaining by an antibody.SELECTED DRAWING: Figure 5

A sample processor including a body including an inner surface that defines a chamber including an opening in a side of the body and including a volume to accommodate a least one microscope slide therein; a door comprising a first position to cover the opening of the body and a second position to expose a portion of the chamber through the opening; a slide bed disposed in the chamber; one of an internal humidity generator disposed in the chamber and an external humidity generator coupled to the chamber. A system including at least one sample processor at least one reagent outside the body of the at least one sample processor and a method including subjecting a sample on a microscope slide in a sealed chamber to a pressure greater than ambient and a humidity greater than 60 percent; and processing the sample.

The application provides a tissue staining method based on antigen repair treatment combined with silver staining, comprising the following steps: a) high-temperature heat repair of a tissue section to be treated with an antigen repair solution, and washing with water after cooling to obtain a repaired tissue section; b) silver staining of the repaired tissue section obtained in step a), dehydration, and then mounting to obtain a stained tissue section. Compared with the prior art, the tissue staining method based on antigen repair treatment combined with silver staining provided by the application adopts specific process steps and conditions to realize overall good interaction: first, the brain tissue is subjected to antigen repair treatment, and then the silver staining technology is adopted, which significantly improves the signal-to-noise ratio of the senile plaque region, and ideal staining effect is obtained, which can effectively improve the detail visibility in pathological detection, help more accurately evaluate the morphology and distribution characteristics of the senile plaque, and has a wide application prospect.

The application belongs to the technical field of pathological tissue processing, and particularly relates to a pathological tissue staining automatic control method and a staining machine system. The method provided by the application detects and calculates the staining quality after each staining, adjusts the staining processing time of hematoxylin and eosin according to the staining quality, and replaces the staining solution if the staining processing time of hematoxylin and eosin exceeds the preset maximum value. The method provided by the application can effectively prolong the service life of the staining reagent, and can also ensure the uniformity and stability of pathological section staining.

This invention relates to the field of soft tissue biomechanical modeling technology, specifically to a constitutive modeling method suitable for J-type soft tissue. The method includes: processing the soft tissue using tissue staining to obtain the volume fractions of matrix, collagen fibers, and elastic fibers; determining an isotropic matrix term based on the characteristics of mesenchymal cells and endothelial cells in the matrix; determining a collagen fiber term based on the characteristics of collagen fiber curling, straightening, fiber rotation, and interfiber slippage; determining an elastic fiber term based on the fiber rotation characteristics of elastic fibers, thus obtaining a model of undetermined parameters; adding the volume fractions of matrix, collagen fibers, and elastic fibers in the soft tissue to the model of undetermined parameters; and using a nearest-nearest global optimization differential evolution algorithm to solve for the undetermined parameters in the model of undetermined parameters, thereby obtaining a hyperelastic constitutive model. This invention can improve the accuracy of the biomechanical property model of J-type soft tissue.

An autonomous quality and hallucination assessment method (AQuA) is disclosed, designed for either virtual tissue staining or histochemical staining. AQuA uses a trained neural network to autonomously achieve 99.8% accuracy when detecting acceptable and unacceptable virtually stained tissue images without access to ground truth, also presenting an agreement of 98.5% with the manual assessments made by a group of board-certified pathologists. AQuA achieves super-human performance in identifying realistic-looking, virtually stained hallucinatory images that would normally mislead human diagnosticians. The wide adaptability of AQuA is demonstrated across various virtually and histochemically stained human tissue images and showcase its strong external generalization to detect unseen hallucination patterns of virtual staining network models as well as artifacts observed in the traditional histochemical staining workflow.

The invention provides application of auramine O in neuronal death and DNA damage detection and a detection method. Auramine O is an ultrafast fluorescent rotor which is rarely applied to tissue staining, can be specifically combined with an endangered or dead neuron, and shows a spatial and temporal distribution characteristic consistent with Fluro-Jade B staining. The technology is extremely sensitive to desoxyribonuclease pretreatment, which indicates that the binding site of auramine O is mainly located in the damaged neuron DNA molecule, so that auramine O not only can detect neuron death, but also can reflect the damage condition of neuron DNA. According to the technical scheme, operation is fast and simple, and the influence of a sample fixing mode is small; the method can be independently applied to detection of neuron death and can also be combined with other fluorescence detection technologies for application. According to the technology, damaged neurons are disclosed, neuron death detection and neuron death mechanism research are combined together, and an effective means is provided for neuroscience research.