Lactobacillus rhamnosus, microbial agent and food product

A technology of Lactobacillus rhamnosus and microbial agent, which is applied in the field of microorganisms, can solve the problems of short duration and inability to reduce the duration and severity, and achieve the effect of expanding the application prospect

Active Publication Date: 2020-05-15
深圳市沁帆科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these treatments only transiently and temporarily relieve the side effects of chemotherapy and cannot reduce the duration and severity of this pathological condition

Method used

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  • Lactobacillus rhamnosus, microbial agent and food product
  • Lactobacillus rhamnosus, microbial agent and food product
  • Lactobacillus rhamnosus, microbial agent and food product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Isolation and identification of Lactobacillus rhamnosus GDMCC No: 60956

[0031] The freshly prepared kimchi was ground to a slurry with a tissue grinding rod, diluted with a gradient of normal saline, spread on an MRS plate containing 1% vancomycin, and placed in an anaerobic incubator at 37°C for 48 hours. After the colony grows out, pick a single colony, separate it by streaking on a plate, carry out repeated purification and culture, and observe the colony morphology by Gram staining method to select Gram-positive bacteria. After the isolated strains were cultured in liquid, they were cultured on a plate, and a single colony was picked with an inoculation loop as a template for colony PCR. The positive PCR products were sent to the company for sequencing.

[0032] The 16S rRNA base sequence analysis and sequencing base sequence of the isolated bacterial strain was compared and analyzed, and it was found to be consistent with the sequence of Lactobacillus...

Embodiment 2

[0035] Example 2: Preparation of Lactobacillus rhamnosus GDMCC No: 60956 fermented milk

[0036] 1 mL of activated Lactobacillus rhamnosus GDMCC No: 60956 strain was inoculated into 100 mL of skim milk (10% w / v). Cultivate under anaerobic conditions at 37°C for 24 hours to obtain a final density of 2×10 9 CFU / mL fermented milk.

Embodiment 3

[0037] Example 3: Effect of Lactobacillus rhamnosus GDMCC No: 60956 on animal injury models

[0038] 40 6-8 week-old female BABL / c mice (provided by the Animal Experiment Center of Nanchang University Medical College) were housed in cages and maintained at a temperature of 22°C ± 2°C and a humidity of 65% ± 5%, with 12-hour light-dark cycle. All animal care and experimental procedures were in accordance with the guidelines of the Department of Animal Science, Nanchang University for the care and use of laboratory animals. The mice were randomly divided into 4 groups (10 animals in each group): CTL group (control), FLRH93 group (control + probiotics), 5-FU group (model group) and 5-FU+FLRH93 group (model + probiotics Group). The CTL group and 5-FU group were fed with 100 μL of skim milk every day, and the FLRH93 group and 5-FU+FLRH93 group were fed with 100 μL of Lactobacillus rhamnosus GDMCC No: 60956 fermented milk every day. To induce gastrointestinal mucositis on day 10,...

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PUM

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Abstract

The invention discloses lactobacillus rhamnosus FLRH93. The lactobacillus rhamnosus FLRH93 is preserved in the Guangdong Microbial Culture Collection Center, and has a preservation number of GDMCC No:60956. Animal damage intervention experiment results show that the lactobacillus rhamnosus FLRH93 can obviously improve the intestinal injury caused by drugs; tissue staining sections show that the strain maintains the integrity of a small intestine structure, reduces the loss of goblet cells after drug treatment, and can regulate and control the secretion of Bcl-2 and NLRP3, thereby reducing generation of inflammatory factors IL1-beta and TNF-alpha. The invention belongs to the technical field of microorganisms. The strain provided by the invention can improve the survival rate of mice withdrug injury and can also remarkably promote healing of wound skin.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a novel Lactobacillus rhamnosus GDMCC No: 60956 (Lactobacillus rhamnosus FLRH93) strain and its probiotic function, especially for protecting damage after clinical treatment. Background technique [0002] Drug therapy is the main diagnosis and treatment method for most clinical diseases, such as 5-fluorouracil (5-FU), as a commonly used chemotherapy drug, is widely used in the treatment of tumors. However, many drugs have side effects and cause damage to the body. For example, after receiving 5-FU and other drugs, more than 50% of patients develop oral mucositis and intestinal mucositis. Damage to the mucosal barrier is one of the most debilitating side effects for patients, often accompanied by symptoms of pain, bacteremia, and malnutrition. These complications are due to decreased intestinal cell proliferation and migration and increased apoptosis, which together disrupt normal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P1/00A23C9/123A23L2/38A23L19/00A23L33/135C12R1/225
CPCA61K35/747A61P1/00A23C9/1234A23L2/382A23L19/00A23L33/135A23V2002/00C12R2001/225C12N1/205A23V2400/175A23V2200/308A23V2200/3204
Inventor 徐锋陈沁胡苗苗
Owner 深圳市沁帆科技有限公司
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