14 results about "Intrinsic fluorescence" patented technology

The application provides an adenosine deaminase content detection method and application thereof. The adenosine deaminase content detection method comprises the following steps: using the intrinsic fluorescence of adenosine, using adenosine deaminase to hydrolyze adenosine, and detecting the content of adenosine deaminase by a fluorescence analysis method. Adenosine itself has strong fluorescence, and after being hydrolyzed by adenosine deaminase, the fluorescence of the obtained hydrolysis product is reduced, thereby causing the fluorescence intensity of the solution of the detection system to be reduced; the content of adenosine deaminase in the system is detected by the change of the fluorescence intensity before and after adenosine hydrolysis. The adenosine deaminase content detection method in the application is simple to operate, does not need large instruments and equipment, and is cheap and easy to obtain materials, can accurately and rapidly detect the content of adenosine deaminase in a sample to be detected, has good sensitivity and specificity, and has a relatively wide application prospect.

The invention relates to the technical field of environment-friendly high polymer materials and functional coatings, and discloses yellow-green fluorescent water-borne epoxy resin for photovoltaic aluminum profiles and a preparation method of the yellow-green fluorescent water-borne epoxy resin. The method comprises the following steps: S1, synthesizing fluorescent dihydric alcohol (NTDI-Diol), S2, synthesizing a fluorescent dicarboxylic acid chain extender (CA-Fluo), S3, taking the CA-Fluo as a chain extender, carrying out ring-opening addition reaction with epoxy resin, and carrying out composite hydrophilic modification and phase inversion emulsification, so as to prepare the waterborne epoxy resin with low-temperature rapid curing, high flexibility and intrinsic fluorescence function. According to the invention, the synergism of technical characteristics and effects is realized through molecular structure design, and the coating can be hard-dried at 5 DEG C for 36 hours, has fluorescence performance and excellent salt fog resistance, is suitable for high-end industrial coating of photovoltaic aluminum profiles and the like, and has a good application prospect.

This invention discloses a quantitative analysis method for microplastics based on a fluorescence detection model and three-dimensional reconstruction technology, belonging to the field of microplastic detection, a typical emerging pollutant. The invention includes preprocessing the microplastic sample to impart intrinsic fluorescence properties, acquiring three-dimensional imaging data using a confocal fluorescence microscope, determining the material density based on the fluorescence fingerprint qualitative results of the microplastic type, and combining the volume data from the three-dimensional imaging reconstruction to achieve quantitative analysis of the microplastic's mass.

The utility model discloses a tumor inherent fluorescence diagnostic apparatus, and relates to the technical field of inherent fluorescence diagnostic apparatuses. The diagnostic apparatus comprises a body, a connecting rope is arranged on one side of the diagnostic apparatus body, a light-guide fiber probe is fixedly installed at the end, away from the diagnostic apparatus body, of the connecting rope, and two fixing seats which are symmetrically distributed are fixedly installed on the side, close to the connecting rope, of the diagnostic apparatus body. A double-thread screw with opposite threads at two ends is rotatably mounted in the middle of the fixed seat, two symmetrically distributed moving blocks are in threaded connection with the outer side of the double-thread screw, and clamping blocks are fixedly mounted at the top ends of the moving blocks; the double-thread screw is arranged to drive the moving block and the clamping block to move towards the middle to clamp and fix the joint of the connecting rope and the diagnostic instrument body, so that the situation that the use of the diagnostic instrument body is affected due to the fact that the joint of the connecting rope and the diagnostic instrument body is separated during use is prevented.

The invention discloses a poly-eutectic stimulus-responsive fluorescent gel, and also discloses a preparation method and application of the fluorescent gel. The preparation method comprises the following steps: mixing a haplotype hydrogen bond acceptor and N-propylene N '-2-hydroxyethyl thiourea to obtain a mixture A, adding deionized water into the mixture A to obtain a mixture B, and heating and stirring the mixture B to obtain a polymerizable eutectic solvent; the preparation method comprises the following steps: adding guanosine, a photoinitiator and a cross-linking agent into a polymerizable eutectic solvent, heating and stirring to obtain a pre-polymerized solution; and casting the pre-polymerized solution in a mold, and initiating polymerization under the irradiation of an ultraviolet lamp to obtain the co-melting-based stimulus-responsive fluorescent gel. The preparation process of the fluorescent gel is green, environment-friendly, simple and efficient, and the obtained gel shows controllable mechanical performance and self-healing performance, has good and stable intrinsic fluorescence characteristics and can be applied to the fields of anti-counterfeiting, information encryption or flexible electronic devices.

The invention discloses ink and a preparation method thereof, a thin film and a photoelectric device, and relates to the technical field of display. The ink comprises a nano material precursor and an alcohol compound; the nano-material precursor comprises Ax (OH) y (NH3) z, A is selected from metal elements, z is 2-6, the valence of A is + w, and x, y and w meet x * w = y; the alcohol compound comprises one or more selected from the following compounds: HO-(CHR1) m-(CHOH) n-(OC4H8) p-(OC3H6) q-(OC2H4) r-(OCH2) s-(CHR2) t-OH and R3-OH. When the ink is used for preparing the film, the nano material precursor can be decomposed to generate the nano material, and the alcohol compound in the ink can reduce the defects of the nano material and prolong the intrinsic fluorescence lifetime of the nano material.

This invention discloses a label-free method for detecting aflatoxin B1 in grains based on intrinsic protein fluorescence. The method utilizes the mechanism of gold nanoparticles enhancing the intrinsic fluorescence of an artificial antigen (aflatoxin B1-bovine serum albumin) on the surface of photonic crystal microspheres. Aflatoxin B1 monoclonal antibody is immobilized on the surface of the gold nanoparticles, and the artificial antigen aflatoxin B1-BSA is immobilized on the surface of the photonic crystal microspheres. After incubating aflatoxin B1 standard or sample extract with the gold nanoparticles and photonic crystal microspheres modified with the above-mentioned biomolecules, the fluorescence signal of the photonic crystal microspheres is detected. The aflatoxin B1 in the sample is qualitatively and quantitatively detected based on the fluorescence signal of the microspheres. This invention can sensitively and specifically detect aflatoxin B1 in samples, reducing detection costs.

The present invention relates to a protein polyacrylamide gel electrophoresis (PAGE)-online intrinsic fluorescence imaging analysis device and method. The device is used for protein electrophoresis separation and online imaging detection of protein bands. The device includes: an open electrophoresis tank including an upper buffer chamber located in the upper part of the open electrophoresis tank, and a lower buffer chamber located in the lower part of the open electrophoresis tank; a UV light source used to excite protein bands in the gel to generate intrinsic fluorescence signals; a CCD imaging detection mechanism including a filter positioned at the front end of the CCD imaging detection device, a UV lens arranged between the filter and the CCD sensor, and a CCD sensor connected to a control unit; a control unit connected to the CCD sensor for online intrinsic fluorescence imaging.

CHITOSAN-MODIFIED DURIAN SEED GUM AS A CANCER CELL BIOIMAGING AGENT This invention relates to a natural polymer material product in the form of polyelectrolyte complex particles composed of durian seed gum and chitosan. The product is made by mixing a solution of durian seed gum and a solution of chitosan to form colloidally stable submicron-sized particles. The particles are responsive to pH changes and exhibit improved kinetic stability and surface characteristics compared to unmodified durian seed gum. The resulting material exhibits intrinsic fluorescence without the addition of external fluorescent agents and has selective activity against cancer cells with high biocompatibility towards normal cells. This invention can be used as a bioimaging material, an intracellular tracking system, a carrier for active compounds, and a supporting material for natural-based cancer therapy.

The invention discloses a colorimetric-fluorescent dual-mode immunoassay method based on a dual-ligand iron metal organic framework (Fe-MOF) nano-enzyme, which is used for detecting protein tumor markers, taking carcinoembryonic antigen (CEA) as an example. The double-ligand Fe-MOF has both peroxidase simulation activity and intrinsic fluorescence, and is coupled with a labeled antibody Ab2 to form Fe-MOF-Ab2; a to-be-detected substance in a sample is captured by the solid-phase coated antibody Ab1 and then forms a sandwich compound with Fe-MOF-Ab2. After H2O2 / TMB is added, Fe-MOF is catalyzed to generate a blue oxidation product (oxTMB) to generate a 652 nm absorbance signal, and meanwhile, oxTMB generates internal filtration / energy transfer quenching on Fe-MOF fluorescence, so that absorbance enhancement-fluorescence weakening dual-channel quantification and mutual verification are realized. The method does not need a natural enzyme or an exogenous fluorescent probe, and is simple, convenient, rapid and reliable to operate.

Methods, systems, and apparatus for determining glomerular filtration rate (GFR) by acquiring a measurement dataset. Furthermore, this disclosure includes: acquiring a measurement dataset comprising multiple measurement entries acquired before and after administration of an exogenous fluorescent agent; generating an intrinsic fluorescence (IF) signal, the IF signal representing the detected fluorescence intensity emitted from the exogenous fluorescent agent within a diffuse reflective medium; determining whether the IF signal includes a portion of early fluorescence data; after determining this portion of the early fluorescence data, identifying an operable IF signal range by filtering the IF signal associated with the early fluorescence data; determining a GFR value in the patient based on the rate of change of the operable IF signal; and providing the GFR value.

The present application belongs to the technical field of epoxy resin, and provides a polycyclic aryl hyperbranched epoxy resin and a preparation method thereof.The preparation method comprises the following steps: performing a branched polymerization reaction on a multifunctional epoxy compound and a polycyclic aryl diphenol under the condition of a catalyst to obtain a polycyclic aryl hyperbranched epoxy resin; the mass ratio of the multifunctional epoxy compound, the polycyclic aryl diphenol and the catalyst is 100:20-60:0.01-0.02.The present application further provides a photoluminescence solution and a corrosion-resistant coating containing the resin.The polycyclic aryl hyperbranched epoxy resin of the present application significantly improves the medium resistance and barrier properties of the coating; the polycyclic aryl hyperbranched epoxy resin has intrinsic fluorescence properties, can predict the failure of the steel corrosion-resistant coating, and can be used for the detection of metal ions.

The application belongs to the field of biomedical detection and molecular imaging technology, and specifically discloses a water-soluble low-background Cy5 probe, a construction method thereof and application of the probe to consistent hypochlorous acid detection across instruments. The probe Cy5Q1-HClO of the application takes Cy5 cyanine dye as a skeleton, realizes near-zero intrinsic fluorescence by means of a double photo-induced electron transfer (PET) mechanism, triggers a self-elimination cascade reaction after specific recognition of hypochlorous acid, and restores strong near-infrared fluorescence. The double-cation structure of the probe endows it with good water solubility, facilitating its use in biological systems. The spectral shift of the probe is extremely small before and after response, only the absorption and fluorescence intensity change, so similar working curves can be obtained on instruments with different excitation and detection channels, the probe has cross-platform and instrument-independent signal quantitative capability, thereby realizing high-sensitivity, zero-background interference and in-vivo precise imaging of HClO. The probe has the characteristics of water solubility, low background and high sensitivity, can not only visualize tumors with high contrast, but also guide precise resection of less than 2mm resection margin, and for the first time realizes longitudinal monitoring of dynamic changes of HClO in the postoperative wound healing process. The application also provides a preparation method and application of the Cy5Q1-HClO probe.

The application provides a manganese-based organic framework and a preparation method and a drug delivery method thereof, wherein the manganese-based organic framework is generated through coordination connection of metal manganese ions and 1,1,2,2,-tetra(4-carboxyphenyl) ethylene ligands, the structure of the manganese-based organic framework is Mn2(TCPE)(H2O), wherein the TCPE is a fluorescent probe 1,1,2,2,-tetra(4-carboxyphenyl) ethylene. The manganese-based organic framework can be used as a nano-carrier with high drug loading capacity, can load active drug molecules with small and medium sizes, and can be used for treating cardiovascular diseases, cancers, microbial infections, wound healing, diabetes, senile dementia, tuberculosis and the like, and has no label and intrinsic fluorescence capacity, and can play a crucial role in actual experiments and interventions for detecting nano-carriers and rapidly tracking drugs.