44 results about "Absolute number" patented technology

The CellTracks® System provides a system to enumerate circulating melanoma cells in blood. The system immunomagnetically concentrates epithelial cells, fluorescently labels the cells and identifies and quantifies circulating melanoma cells. The absolute number of circulating melanoma cells detected in the peripheral blood tumor load is, in part, a factor in prediction of survival, time to progression, and response to therapy. Diagnosis and monitoring of melanoma has been limited by the inability to monitor circulating melanoma cells. The present invention provides a method to enumerate circulating melanoma cells in blood samples. Accordingly, this technology provides a means and device for monitoring disease progression in patients with melanoma.

A cancer test having prognostic utility in predicting time to disease progression, overall survival, and response to therapy in patients with MBC based upon the presence and number of CTC's. The Cell Spotter® System is used to enumerate CTC's in blood. The system immunomagnetically concentrates epithelial cells, fluorescently labels the cells and identifies and quantifies CTC's. The absolute number of CTC's detected in the peripheral blood tumor load is, in part, a factor in prediction of survival, time to progression, and response to therapy. The mean time to survival of patients depended upon a threshold number of 5 CTC's per 7.5 ml of blood. Detection of CTC's in metastatic cancer represents a novel prognostic factor in patients with metastatic cancers, suggests a biological role for the presence of tumor cells in the blood, and indicates that the detection of CTC's could be considered an appropriate surrogate marker for prospective therapeutic clinical trials.

The present invention relates to pharmaceutical kits and methods to treat lung inflammation and redox imbalance in human cystic fibrosis patients using pharmaceutical compositions containing N-acetylcysteine (NAC), pharmaceutically acceptable salts of N-acetylcysteine, or N-acetylcysteine derivatives. In phase I studies, treatment with oral NAC at a dose of from about 1800 mg / day to about 3000 mg / day for a period of 4 weeks produced significant positive effects, namely, it decreased absolute numbers of white blood cells and neutrophils in the sputum and produced concomitant decreases in sputum neutrophil elastase specific activity and sputum interleukin-8 levels, suggesting an amelioration of lung inflammation in the patients. These effects were associated with an increased total GSH level in whole blood as well increased staining for reduced GSH in blood neutrophils, both of which reflect an amelioration of the redox imbalance in the patients. In ongoing phase II studies, oral NAC at a dose of about 2700 mg / day administered in double-blind manner for 12 weeks showed excellent safety and significantly decreased white blood cells in sputum as compared to placebo.

The invention relates to the medicines for gynecopathy, in particular to gel for adjusting microecology in vaginas of women, solving the problem that the traditional gynecological lotion kills probiotics while killing pathogens, thus causing vaginal flora imbalance and low immunity and further causing repeated vaginitis. The gel comprises the following components according to mass concentration: 0.5-2% of Carbomer, 0.1-1% of soy peptide, 0.001-0.01% of isoflavone, 0.5-3.5% of carotene and 0.3-3% of tea tree oil. The gel achieves the aim of suppressing pathogens by promoting lactobacillus proliferation. At present, the products in the market aim to kill the bacteria but finally disturb normal flora balance. The gel increases the absolute number of the lactobacillus, a predominant bacterium, simultaneously reduces the pathogens, maintains the balance of microecology in vaginas and improves vaginal cleanliness and the immunity of the vagina mucosa, thus achieving the aims of preventing gynecological vagina inflammation and reducing the reoccurrence rate of gynecological vaginitis. Besides, the formula of the gel is pure vegetable protein, thus being safer than other bacteriostats and bactericides.

Control beads are disclosed that allow for improved quantitation of analytes in multiplexed bead assays. The control beads have a range of concentrations of calibration moieties that provide for the preparation of a titration curve. The titration curve can be used to quantify the concentration of the analytes. The titration curve can be used to correlate the signal obtained from a bead with the concentration (or absolute number of molecules) of the analyte bound to the bead.

The invention discloses a method and a device for pushing information to a mobile terminal, which can determine positions in which a user is possible to take interest according to contact information saved by the user, and recommend the positions to the user according to a certain order. According to the technical scheme of the invention, contact information saved in different apps in the mobile terminal of the user is collected and summarized for analysis; relation between various apps is taken into consideration, so that the potential function of the information is exerted to the maximum degree, and the information resource utilization ratio is increased. Particularly, the positions can be sorted according to the absolute number of occurrence times of each position or the contact frequency of the contact and the user, and pushed information from a region which the user wants most is placed at the top of the sequence, so that the user is free from searching by oneself, blindness during user choosing is reduced, time is saved for the user, convenience is brought to the user to acquire pushed information from other regions, and the user experience is improved.

A Residual Time Stamp (RTS) technique provides a method and apparatus for recovering the timing signal of a constant bit rate input service signal at the destination node of a synchronous ATM telecommunication network. At the source node, a free-running P-bit counter counts cycles in a common network clock. At the end of every RTS period formed by N service clock cycles, the current count of the P-bit counter, defined as the RTS, is transmitted in the ATM adaptation layer. Since the absolute number of network clock cycles likely to fall within an RTS period will fall within a range determined by N, the frequencies of the network and service clocks, and the tolerance of the service clock, P is chosen so that the 2P possible counts, rather than representing the absolute number of network clock cycles an RTS period, provide sufficient information for unambiguously representing the number of network clock cycles within that predetermined range. At the destination node, a pulse signal is derived in which the periods are determined by the number of network clock cycles represented by the received RTSs. This pulse signal is then multiplied in frequency by N to recover the source node service clock.

The invention belongs to the technical field of high polymer materials and in particular relates to a method for preparing a high-performance thermoplastic polyurethane elastomer. According to the method, a novel idea is adopted, the defect that the molecular weight of a polyurethane elastomer is controlled by a single R value is avoided, although preparation of certain molecular weights of thermoplastic polyurethane elastomers sealed by different groups can be stilled affected by factors such as equipment metering precision and moisture at an early stage, the molecular weights can be remarkably increased when being compared with those of early-stage products after two times of polymerization at a later stage, and the viscosity and the mechanical properties can be remarkably improved; themethod is simple in production process and high in operability, and the absolute number-average molecular weight of the prepared thermoplastic polyurethane elastomer is 80.000 g / mol or greater (GPC (Gel Permeation Chromatography), light scattering methods).

The invention relates to the medicines for gynecopathy, in particular to physiological balance liquid for adjusting microecology in vaginas of women, solving the problem that the traditional gynecological lotion kills probiotics while killing pathogens, thus causing vaginal flora imbalance and low immunity and further causing repeated vaginitis. The physiological balance liquid is formed by major materials, auxiliary materials and water, wherein the major materials include the following components according to mass concentration: 0.7-1% of sodium chloride, 0.01-0.04% of potassium chloride, 0.01-0.04% of calcium chloride, 0.01-0.04% of magnesium sulfate, 0.001-0.01% of manganese sulfate and 0.01-0.05% of glucose; and the auxiliary materials include the following components according to mass concentration: 0.1-1% of soy peptide, 0.001-0.01% of isoflavone, 0.05-0.6% of tea tree oil and 0.002-0.04% of carotene. The physiological balance liquid conforms to physiological conditions of the vagina mucosa, increases the absolute number of the lactobacillus, a predominant bacterium, reduces the pathogens, maintains the balance of microecology in vaginas and improves vaginal cleanliness and the immunity of the vagina mucosa, thus achieving the aims of preventing gynecological vagina inflammation and reducing the reoccurrence rate of gynecological vaginitis. The physiological balance liquid has good biocompatibility and no irritation or side effects and is safe.

A method for determining absolute number densities of particles in a solution is disclosed based on a light scattering method. A light scattering photometer is calibrated to produce the Rayleigh ratio at each angle measured with respect to light scattered per unit incident intensity, per unit volume illuminated within the field of view of each detector per steradian subtended by said detector. In order that the numbers calculated be accurate, the illuminated particles should be effectively monodisperse. From the excess Rayleigh ratios measured at a plurality of angles with respect to the incident light beam illuminating said sample particles, an effective size is calculated which, in turn, is used to calculate the differential scattered intensity at each angle. The number of particles per unit volume element is then determined from the measured excess Rayleigh ratio divided by the corresponding differential scattered intensity.

The invention relates to a peripheral blood lymphocyte subpopulation cell concentration detection kit and a detection method thereof, and belongs to the technical field of cell detection and screening. According to the technical scheme, the peripheral blood lymphocyte subpopulation cell concentration detection kit comprises an antibody kit and a stimulation liquid kit which is coordinately used with the antibody kit and is characterized in that a six-hole reagent tank is stored in the antibody kit, reagent tubes ranging from number A to number F and representing six antibody solutions are correspondingly arranged in the six-hole reagent tank according to the numbers of the six-hole reagent tank, and a standby reagent bottle is further stored in the antibody kit; after the detection of peripheral blood lymphocyte subpopulation cell concentration and the detection of CD4+T cell subpopulation cell concentration are carried out, samples are placed on a flow cytometer to analyze flow cytological maps, relative percentage is acquired and analyzed by MultiSET software or CellQuest software, and the cell absolute number of various subpopulations is calculated. The peripheral blood lymphocyte subpopulation cell concentration detection kit has the advantages that the technical problem that cell detection is large in errors and complicated in steps is solved, and the detection kit is capable of providing various antibodies and reagents, capable of simultaneously testing a plurality of samples during clinical application, simple in test steps, good in quality control and high in cell detection precision.

The present specification discloses methods of characterizing a population of particles using a particle analyzer. Particles may be characterized by size, absolute number, whether the particle is non-proteinaceous or proteinaceous, whether a proteinaceous particle has or lacks a certain physical property, or any combination thereof.

The invention relates to the technical field of medical care, aims at solving the problem that vaginitis is repeatedly caused since the traditional gynecological lotion kills many probiotics while killing pathogenic bacteria and causes vaginal flora imbalance and low vaginal immunity, and provides a gynecological vaginal lotion for adjusting the female vaginal micro-ecosystem and a preparation method thereof. The preparation method comprises the following steps: adding natural perfume having 0.01-0.5 percent of the volume concentration of acidic water into the acidic water, disinfecting, and adjusting the pH value to be 3.5-5.5. According to the method, lactobacillus reproduction is promoted to achieve the aim of inhibiting pathogenic bacteria, the absolute number of dominant bacterium lactobacillus is increased, the number of pathogenic bacteria is reduced, the micro-ecological balance of vagina is maintained, the vaginal cleanness is improved, the vagina mucosa immunity is improved, the gynecological vaginal inflammation is prevented, and the recurrence rate of gynecological vaginitis is reduced. The gynecological vaginal lotion is safe, has a specific pH value range being 3.5-5.5, and is safer and more effective than those of other bacteriostatic agents and bactericides.

The invention discloses a stem-cell-like memorability T cell in-vitro inducer and a method. The inventor finds through experiments that a CD8 initial T cell can be rapidly induced, differentiated and amplified into TSCM cells in vitro if a histamine H1 receptor antagonist is additionally added into a T cell induction medium, the ratio of the TSCM cells to the CD8 cell can be remarkably increased, and the absolute number of the TSCM cells can be remarkably increased. Properties and functions of the induced, differentiated and amplified TSCM cell are maintained. The security of the histamine H1 receptor antagonist to human bodies is already tested through clinical practice, and a long research and development cycle of a new drug can be avoided. Meanwhile, as a developed medicine, the histamine H1 receptor antagonist has the advantages of low cost and good security, the TSCM cells induced, differentiated and amplified with the histamine H1 receptor antagonist can be re-transfused without special treatment, a powerful practice basis can be provided for tumor cell treatment, and significant development values and popularization meanings can be achieved.

The CellTracks® System provides a system to enumerate CTC's in blood. The system immunomagnetically concentrates epithelial cells, fluorescently labels the cells and identifies and quantifies CTC's. The absolute number of CTC's detected in the peripheral blood tumor load is, in part, a factor in prediction of survival, time to progression, and response to therapy. Pre-clinical studies of circulating tumor cells (CTC's) have been limited by the inability to repetitively monitor CTC's in animal models. The present invention provides a method to enumerate CTC's in blood samples obtained from living mice, using a protocol similar to an in vitro diagnostic system for quantifying CTC's in patients. Accordingly, this technology can be adapted for serial monitoring of CTC's in mouse xenograft tumor models of human breast cancer.

The invention discloses a laser induced plasma parameter characterization method based on self-absorption quantification of a spectral line. The self-absorption degree of the spectral line is quantified by measuring the line width of the element spectral line so as to obtain plasma parameters such as electron temperature, element content ratio and particle absolute number density. Because the calculation process of the method has no need of using spectral line intensity, adverse influences caused by the self-absorption effect during traditional calculation of the plasma parameters based on thespectral line intensity are avoided, and the spectral efficiency of a detector does not need to be corrected so as to effectively prolong the calibration period of LIBS (Laser-Induced Breakdown Spectroscopy) detection equipment and directly and accurately characterize the plasma parameters.

The invention relates to the technical field of immunocyte targeting therapy, in particular to an optimized kit system and application for separating and purifying memory immune cells (TCM) or CD8T+ cells from human peripheral blood or peripheral blood mononuclear cells, a TCM induction and amplification kit and a kit for product quality control and identification. In clinical application, TCM cells were cultured with many reagents, inconsistent standards, as that culture process has the advantages of large workload, long culture time, tedious process, instability, high contamination risk anddifficult control of cell quality standards, it is difficult for many technicians to culture TCM cell with uniform quality in a short period of time, and it is urgent to develop a simple and formattedkit. Meanwhile, the invention further optimizes the inducer composition TCM-III, the raw material is saved and the production cost is reduced; TCM-II-coated culture plate increased the proliferationof TCM cells by 2-3 times, the apoptotic index decreased, and the activity is increased by 10%-20%, the absolute number of effective cells increased by 2-3 times; before TCR gene transfection, TCM-II-coated culture plate can improve the transfection efficiency. The invention improves the process, which has important significance for clinically improving the curative effect of tumor treatment, removing residual lesions, and preventing the recurrence and metastasis of tumor.

The invention discloses a gasoline motor spark plug with radiation fins and a cranked electrode. The radiation fins are made from conventional steel by maintaining a part of the steel which is originally cut. At least one, or two, or three or a plurality of radiation fins are arranged. The hexagonal shape of the radiation fin endows the radiation fin with the advantages that: 1, the radiation fin is convenient to machine together with a hexagonal spanner nut on the radiation fin by one cutter; and 2, the radiation fin is convenient for the deeper sleeving of a sleeve spanner in mounting. As an improvement, the gasoline motor spark plug particularly improves an ordinary single flat electrode into the cranked electrode (16), wherein the cranked electrode (16) consists of an extended part (16a), an arched part (16b) and a cranked part (16c) which are an equal distance away from a discharge electrode so as to form a discharge gap between a charged electrode and the discharge electrode; and the discharge gap is longer than a conventional line, which is favorable for increasing the absolute number of mixed gases in the gap and increasing the ignition probability.

A method for compressing a string of characters, initially defined by an original ordered sequence of characters each represented by an absolute number uniquely representing each character, according to an absolute coding table, comprising the steps of: - parsing said original sequence into at least one first type sub-sequence comprising contiguous characters, each of the contiguous characters of the first type sub-sequence being selected based on a matching character in a relative coding table (20), and, into at least one second type sub-sequence comprising remaining contiguous characters, - coding each first type sub-sequence using said relative coding table (20), - representing each character from each second type sub-sequence by its absolute representing number from said absolute coding table. A corresponding method for decompressing a compressed sequence.

The invention relates to an angle-measuring device for a rotationally driven linear actuator, in particular for a clutch actuator, which has at least the following components: - a rotor element whichhas an axis of rotation and rotates concentrically with a rotor of a rotatory drive for an axially movable linear actuator element; - a first measurement magnet arrangement which is fixed relative tothe rotor element and has a polarization, the polarization being oriented in such a way that the magnetic field lines can change with a rotation of the rotor element so as to enable the angle to be precisely determined from at least one measurement position; - at one of the measuring positions, a 360-degree sensor for measuring angular positions based on the measurement magnet arrangement; at oneof the measurement positions, a revolution-counting sensor for counting an absolute number of revolutions carried out based on the measurement magnet arrangement. The angle-measuring device proposed here ensures fail-safe reading out of the absolute travel position of, for example, a master piston of a hydrostatic clutch actuator and has low space requirements.

The present invention discloses applications of interleukin-21 in preparation of a stem cell-like memory T cell in vitro amplification inducer. According to the present invention, with the addition of interleukin-21 as an inducer to a T cell induction culture medium, the differentiation amplification of Naive CD8+ T cells can be induced to obtain TSCM cells, the forming proportion and the absolute number of the TSCM cells are significantly increased, and the effect is significantly superior to the effect of the inducer IL-2 universally used in the cell adoptive immunotherapy; and the interleukin-21 is the natural cytokine secreted by human body, has the high safety, does not have the drug toxicity and other problems, can provide the good technical support for the extensive development of the adoptive immunotherapy, and has the important promotion significance.

A flow cytometry method and kit is provided for quantifying an absolute number of mature sperm cells in a semen sample, the method comprising (a) determining a total particle number (T); (b) determining a number of DNA comprising particles (D); (c) determining a number of cells which are not mature sperm cells (B) and; (d) determining a background signal (A); wherein steps (a) to (d) are not effected in a single aliquot of the semen sample, and whereas the number of mature sperm cells in the semen sample is equal to [(T×D) / 100]−(A+B), thereby quantifying the absolute number of mature sperm cells in the semen sample.

A flow cytometry method and kit is provided for quantifying an absolute number of mature sperm cells in a semen sample, the method comprising (a) determining a total particle number (T); (b) determining a number of DNA comprising particles (D); (c) determining a number of cells which are not mature sperm cells (B) and; (d) determining a background signal (A); wherein steps (a) to (d) are not effected in a single aliquot of the semen sample, and whereas the number of mature sperm cells in the semen sample is equal to [(T×D) / 100]−(A+B), thereby quantifying the absolute number of mature sperm cells in the semen sample.

In a method for determining an absolute number of electron spins in an extended sample (3) with the assistance of an apparatus for measuring magnetic resonance, the extended sample (3) is disposed within a measurement volume (2) of a radiofrequency RF resonator (1) of the apparatus during an electron spin resonance measurement (ESR). The method has the following steps: determining a spatial sensitivity profile f of the RF resonator (1) over the measurement volume (2); determining a resonator sensitivity constant c by means of a comparison to the measurement volume (2) of small calibration sample having a known number of electron spins at a particular position within the measurement volume (2); measuring a magnetic resonance signal RS of the extended sample (3) in the apparatus with a known spatial distribution of extended sample (3) within the measurement volume (2); weighting the magnetic resonance signal RS with the integral of the spatial sensitivity profile f of the RF resonator over the partial volume of the measurement volume (2) occupied by the extended sample (3); and determining the number of electron spins NS in extended sample (3) as a quotient between the weighted resonance signal and the resonator sensitivity constant c. The method facilitates a simpler determination of the absolute number of electron spins in the sample.

A position-measuring device includes: a first subassembly having a measuring standard on which at least one coded track is provided, and a scanning unit, which is able to generate position signals that may be used to generate an absolute digital position value by scanning the at least one coded track in a measuring direction; a second subassembly having at least one peripheral unit adapted to execute a supplementary or an auxiliary functionality of the position-measuring device; and a plurality of electrical lines, which connect the first subassembly and the second subassembly to each other for the transmission of electrical signals. The position-measuring device is able to be operated in an initialization mode and in a standard operating mode. All components of the first subassembly required for the operation in the standard operating mode are components that are suitable for use in a radiation region of a machine. Furthermore, an initialization memory is provided in the first subassembly, which includes the data required for the operation in the standard operating mode and which is not suitable for use in a radiation region of a machine. In the initialization mode, the content of the initialization memory is transmittable to a memory unit situated outside the radiation region. Only the content of the memory unit is used for the operation in the standard operating mode.

Control beads are disclosed that allow for improved quantitation of analytes in multiplexed bead assays. The control beads have a range of concentrations of calibration moieties that provide for the preparation of a titration curve. The titration curve can be used to quantify the concentration of the analytes. The titration curve can be used to correlate the signal obtained from a bead with the concentration (or absolute number of molecules) of the analyte bound to the bead.

An absolute position-measuring device includes a first subassembly having a measuring standard on which at least one code track is disposed, and a scanning unit with which, by scanning the at least one code track in a measuring direction, position signals are able to be produced, from which an absolute digital position value is able to be generated. The position-measuring device includes a second subassembly having at least one peripheral unit adapted to perform an additional or auxiliary function of the position-measuring device. The first subassembly and the second subassembly are connected to each other by a plurality of electric lines for the transmission of electrical signals. The first subassembly exclusively includes components which are suitable for use in a radiation area of a machine.

Disclosed is a method and apparatus for determining stone cells within a sample of wood pulp or paper. According to the instant invention a portion of the sample is irradiated with light at a predetermined wavelength or within a predetermined range of wavelengths. The incident light causes those areas of the sample that are occupied by stone cells to fluoresce strongly relative to the background matrix of wood pulp or paper. The strongly fluorescing stone cells are registered digitally using a detector, or are observed visually by an operator viewing the sample through an ocular device. The absolute number of stone cells per unit area or a stone cell size-distribution-plot is obtained using one of automated or manual image analysis.

An absolute position-measuring device includes a first subassembly having a measuring standard on which at least one code track is disposed, and a scanning unit with which, by scanning the at least one code track in a measuring direction, position signals are able to be produced, from which an absolute digital position value is able to be generated. The position-measuring device includes a second subassembly having at least one peripheral unit adapted to perform an additional or auxiliary function of the position-measuring device. The first subassembly and the second subassembly are connected to each other by a plurality of electric lines for the transmission of electrical signals. The first subassembly exclusively includes components which are suitable for use in a radiation area of a machine.

The invention discloses an electronic identity system for a power storage battery of an electric vehicle. The electronic identity system is installed on a battery pack of a pure electric vehicle or a hybrid vehicle with a storage battery as one of the power sources and cannot be disassembled. The electronic identity system not only endows the power storage battery with a unique battery electronic identity, but also stores the actual remaining power, geographical location, used The vehicle identity information of the electric vehicle and the absolute number of kilometers driven of the electric vehicle used. All the data stored above are continuously uploaded to the file server or cloud server through the peripheral sub-terminal. The present invention collects and provides necessary basic parameters for the quantified management of the traction storage battery, so that the fair and reasonable exchange and use of the traction storage battery of the electric vehicle becomes a feasible solution.