110 results about "Circulating cancer cell" patented technology

The invention belongs to the technical field of biological intelligent detection, and particularly relates to a circulating tumor cell automatic detection device based on a micro-fluidic nano chip and AI analysis and application. The device comprises a micro-fluidic chip module, an optical acquisition module, an image recognition module, a feedback control module and a liquid path control module, and can realize efficient enrichment, image recognition and dynamic fluidic regulation of circulating tumor cells in peripheral blood. By constructing a change trend model of an image frame sequence, cell state changes are recognized in real time, flow velocity and channel control instructions are output, and collaborative optimization of recognition precision and the sample utilization rate is achieved. The device is suitable for tumor early screening, curative effect monitoring and recurrence early warning scenes.

The invention relates to the technical field of medical information, and particularly discloses a blood transfusion strategy optimization system based on clinical indexes and molecular markers, which comprises the following steps: firstly, collecting clinical indexes of a patient and molecular marker data derived from circulating tumor cells; standardized integration is carried out to construct a multi-dimensional feature set reflecting correlation between clinical dynamics and molecular features; key association rules are identified through data mining, quantitative abnormal expression profiles and risk hierarchical labels are generated for each patient according to the key association rules, the blood transfusion urgency state is evaluated in combination with real-time data, physiological responses and risk changes under different blood transfusion schemes are deduced, and the optimal individual blood transfusion strategy parameters are solved. Performing deduction verification on the strategy by constructing a patient-specific virtual simulation scene, comparing the strategy with historical data to calculate an expected efficiency deviation degree, and outputting a final execution scheme subjected to optimization verification; according to the invention, the transformation from static threshold transfusion to individualized dynamic prediction and optimized transfusion is realized.

The invention belongs to the technical field of pharmaceutical preparations. The invention discloses a multifunctional bionic nano preparation, a preparation method and application. According to the multifunctional bionic nano preparation, the ginsenoside Rg3 has the dual functions of a medicine and a membrane stabilizer, the platelet membrane has the natural targeting capability on circulating tumor cells and metastases, and meanwhile, the cationic liposome is used for adsorbing negatively-charged NETs nucleic acid protein compounds, so that potential reversal and active targeting are achieved, and the multifunctional bionic nano preparation has a good application prospect. And by co-carrying paclitaxel (PTX) and a photothermal agent (PCP) and integrating photothermal-chemotherapy-immunogenic death (ICD) induction functions, the tumor-related fibroblast activation can be effectively inhibited, circulating tumor cells can be efficiently captured, a tumor immune microenvironment can be remodeled, the tumor cells can be effectively killed, and tumor distal metastasis can be inhibited.

The invention relates to the technical field of computational fluid mechanics and biological microfluidic design, in particular to a model order reduction method for rapid optimization of a circulating tumor cell (CTC) sorting structure, which comprises the following steps of: constructing a three-dimensional full-order computational fluid mechanics model by collecting channel geometric structure parameters and fluid working condition parameters, and generating a training data set; organizing a flow field snapshot into a matrix form, extracting a dominant mode, constructing a low-dimensional modal space by taking the dominant mode as a base vector, establishing a low-dimensional ordinary differential model through Galerkin projection, and training a parameter-modal coefficient mapping relation by adopting a deep neural network to form a complete reduced-order model; and finally, constructing a multi-objective optimization problem based on the reduced-order model, carrying out optimization iteration by adopting an evolutionary algorithm, and returning an optimization result to the full-order model for verification, so that rapid optimization design of the CTC sorting structure is realized, and an effective solution is provided for intelligent design of a biomedical microfluidic device.

The invention relates to a circulating tumor cell enrichment, separation and dyeing integrated system and application thereof.The circulating tumor cell enrichment, separation and dyeing integrated system is composed of a bottom plate module, a platform module, a multi-pipette module, a power module and a shell module; the platform module comprises a sample tube rotating and uniformly mixing module, a magnetic adsorption module, a reagent module, a waste liquid pool module and a gun head module, and the sample tube rotating and uniformly mixing module, the magnetic adsorption module and the reagent module are fixedly mounted on a bottom plate I in sequence. The CTC separation and dyeing integrated system is developed on the basis of the immunomagnetic bead technology, enrichment separation and immunofluorescent dyeing of CTC can be automatically completed by running a program, manual operation errors are effectively avoided through a programmed process, and the CTC separation and dyeing integrated system is particularly suitable for clinical CTC detection. The system is also suitable for other enrichment and separation applications based on an immunomagnetic bead technology, including but not limited to enrichment and separation of biological macromolecules such as cells, exosomes, proteins and the like.

This invention relates to a method and application for enriching circulating tumor cells (CTCs) based on ambient temperature mechanical perfusion technology, belonging to the field of biomedical technology. The method comprises the following steps: flushing the liver resected during liver transplantation via the portal vein and hepatic artery, connecting it to an ambient temperature mechanical perfusion loop, and performing in vitro perfusion using simulated blood perfusion fluid; when perfusion exceeds 2 hours and the lactate content drops to <5 mmol / L, collecting the simulated blood perfusion fluid; and sequentially subjecting the simulated blood perfusion fluid to centrifugation, red blood cell removal, and white blood cell removal to obtain enriched circulating tumor cells (CTCs) for liver cancer. A human liver cancer ex vivo organ perfusion model is constructed using NMP technology to fully recreate the tumor microenvironment and CTC generation process, and the large volume of perfusion fluid is processed to achieve efficient enrichment of CTCs.

The utility model discloses a kind of circulating tumor cell in vitro culture fluid filtering devices, including shell, the bottom surface of the shell is fixedly installed with rotating motor, the inner bottom wall of the shell is inlayed with first bearing, the inner ring of the first bearing is fixedly connected with rotating rod, the output end of the rotating motor is connected with the bottom end of rotating rod by first bearing, the upper surface of the rotating rod is fixedly connected with clamping block. The cooperation of the device through clamping groove and clamping block, realize rotating motor and filter frame flexible separation, by inserting the insert block of cover plate bottom into storage groove, and using positioning assembly to position cover plate, make synchronous assembly extrude filter frame, the phenomenon of displacement occurs when filter frame rotates, realize that filter frame is conveniently disassembled and installed, to facilitate subsequent maintenance and cleaning, to solve the disassembly of the filter screen of existing device is more cumbersome, leading to the problem of lower work efficiency.

The invention belongs to the technical field of anti-tumor drugs, and particularly relates to a flexible drug-loaded cell microparticle capable of efficiently targeting tumors and a preparation method of the flexible drug-loaded cell microparticle. Comprising tumor cell-derived drug-loaded microparticles coated with chemotherapeutic drugs, and a flexible regulator modified on the surfaces of the tumor cell-derived drug-loaded microparticles, the flexible regulator comprises a saponin compound and / or a steroid compound, and can regulate and control the hardness of the tumor cell-derived drug-loaded microparticles. The tumor cell-derived drug-loaded microparticles are modified, so that the enrichment and deep penetration of the drug-loaded microparticles in tumor tissues can be remarkably improved, and the efficient targeted delivery of the tumor tissues is realized. Meanwhile, efficient uptake of tumor stem cells to the medicine is promoted, targeted killing is achieved, the tumor stem cells are effectively removed, and tumor growth is remarkably inhibited. In addition, circulating tumor cells can be efficiently targeted, accurate delivery of tumor metastasis is achieved, and tumor metastasis is remarkably inhibited by inhibiting migration and invasion of tumor cells and reducing formation of lung metastasis.

The application provides a kind of bacterial outer membrane vesicle based on acid-sensitive peptide label and its preparation method and application, it is related to the technical field of engineered bacterial outer membrane vesicle.The ClyA-pHLIP fusion protein is designed and synthesized in the application, and the pHA-W3110OMV stably expressing the ClyA-pHLIP fusion protein is successfully obtained by using genetic engineering technology.The pHA-W3110OMV effectively solves the problem of easy aggregation of pHLIP in acidic environment, has good stability and solubility, and provides conditions for the stable targeting performance of pHLIP.Meanwhile, the pHA-W3110OMV not only retains the characteristics of targeting tumor cells in acidic environment, but also realizes the characteristics of targeting tumor cells in non-acidic environment.The pHA-W3110OMV has high targeting tracing efficiency on tumor cells under different pH conditions, and can realize qualitative and quantitative detection of tumor cells in peripheral blood, and has application potential in circulating tumor cell detection and tumor primary and metastasis localization.

The invention relates to the technical field of circulating tumor cell enrichment and automation equipment, in particular to an automatic circulating tumor cell enrichment device. A rectangular disc, a rectangular rod, a placement disc, a chip storage box, a waste liquid outlet pipe, a cover plate, a reagent storage unit, a waste liquid transfer pipe and a waste liquid storage box are arranged; by rotating the rectangular disc at each rotation degree, each chip storage box on the placement disc can directly face a worker to facilitate placement of chips, but the closed opening of the chip storage box closest to the transfer frame does not directly face the worker and is to be subsequently rotated to directly face the worker, so that the chips are placed in each chip storage unit, and the chip placement efficiency is improved. After all the chips are placed, a stepping motor is started, the stepping motor enables a rectangular disc to rotate intermittently, and then the cleaning liquid or the actual liquid in the chip storage box is vibrated to be used for fully conducting cleaning or enrichment reaction on the chips in the chip storage box; according to the invention, one-time enrichment can be carried out on at least fifty chips at one time.

This invention belongs to the field of cell sample detection and provides a microfluidic chip for separating circulating tumor cells (CTCs) based on size and aptamers. The invention includes a functional layer with a sample outlet and a sample inlet, and a cell substrate disposed on the upper and lower surfaces of the functional layer. The sample outlet and sample inlet of the functional layer are connected by a separation channel, which includes at least one micropillar array capable of carrying an EpCAM aptamer. The micropillar array is composed of a capture channel surrounding at least two micropillar structures. One end of the micropillar array is connected to the sample inlet, and the other end of the micropillar array has a gradient retention channel connected to the sample outlet. The microfluidic chip uses EpCAM aptamers to modify the micropillar array, increasing the number of collisions between CTCs and the chip, and increasing the probability of collisions between circulating tumor cells and the aptamers on the micropillar surface. Secondly, by utilizing the high specificity of the aptamer for tumor cells, the accuracy and specificity of separation are improved.

Provided is a device for screening and separating circulating tumor cells (CTCs), comprising: a chamber module, the chamber module being provided with a mixing chamber, a screening chamber and a waste liquid chamber, and the mixing chamber, the screening chamber and the waste liquid chamber being sequentially communicated; a control module, the control module being mounted on the chamber module, and the control module being configured to control, according to the motion state thereof, whether to discharge a liquid from the mixing chamber to the screening chamber; a temperature control module, the temperature control module being mounted on the chamber module, and the temperature control module being configured to control the temperature in the mixing chamber; and a magnetic enrichment module, the magnetic enrichment module being configured to form a magnetic field in the mixing chamber. Provided is the device for screening and separating CTCs on the basis of immunomagnetic bead separation technology. The device has a compact structure and a small size, is convenient to operate, and has the function of simulating the constant-temperature environment in human bodies to assist in reaction incubation, thereby ensuring the reliability and precision of detection.

Disclosed herein are nucleic acid constructs that can be used to build genetic circuits for producing antibodies comprising split toxins. Also disclosed herein are methods of producing platelets comprising the antibodies. The platelets produced by the methods disclosed herein can be used to target circulating tumor cells.

Disclosed are methods for detecting and treating cancers (such as carcinoma, leukemia, lung cancer, colon cancer, central nervous system (CNS) cancer, melanoma, ovarian cancer, renal cancer, oral cancer, prostate cancer and / or breast cancer). The method may include receiving a sample from a patient (such as a sample from a biopsy, a sample from an extracellular vesicle, or a sample from a circulating tumor cell). The method may include determining whether a LY6C protein or a phosphorylated ANXA2 protein is detected in the sample. The method may include treating a patient for cancer by targeting the LY6C protein when the LY6C protein is detected (e.g., using photoimmunotherapy (PIT) or near infrared photoimmunotherapy (NIR-PIT)). or treating a patient for cancer by targeting an ANXA2 protein (e.g, using inhibitors, decoys, etc.) when the phosphorylated ANXA2 protein is detected.

The invention relates to the field of circulating tumor cell separation, particularly discloses a circulating tumor cell separation device based on a magnetic field, and aims at solving the problems that an existing magnetic separation device is poor in use universality, the internal circulation is easily influenced, and the separation efficiency is relatively low. The magnetic separation device comprises a base frame, lantern rings, a frame cylinder, a separation piece, a liquid inlet pipe, a discharge pipe, a liquid discharge pipe, a communication cavity, a first limiting sliding groove, a first blocking block, a second limiting sliding groove, a second blocking block, a first electromagnet, a second electromagnet, a sliding opening, a sliding plate, a shifting plate and a magnetic separation assembly, and the two sides of the base frame are each slidably provided with a sleeve plate matched with the upper end face of the shifting plate in an abutting mode; a first control assembly for controlling the first plugging block is arranged on the sleeve plate on the left side, and a second control assembly for controlling the second plugging block is arranged on the sleeve plate on the right side. The device can change the diameter of a flow channel in the separation liquid inlet pipe, facilitates real-time and efficient separation, can flexibly adjust magnetic force, and is high in universality and more flexible to use.

The application discloses a lock-key type DNA assembly for circulating tumor cell marker protein multiplex analysis, and belongs to the technical field of biomedical molecular diagnosis. The lock-key type DNA assembly comprises a lock type DNA assembly and a key type DNA. The lock type DNA assembly comprises a 16nt double-stranded displacement region, a 10nt single-stranded branch point region, a 7nt single-stranded closed region and a covalent double-stranded signal region containing a plurality of fluorescent groups. The key type DNA is a single-stranded sequence modified on an antibody. The application realizes in-situ signal amplification and CTC marker protein multiplex analysis on a single cell level based on the lock-key type DNA assembly design, provides a theoretical basis for circulating tumor cell multiplex detection analysis of cancer, and provides a new method for further realizing precise diagnosis and individualized treatment of cancer.

The invention relates to the technical field of biomedical engineering, and discloses a detection method for esophageal squamous carcinoma CTC enrichment and immunotherapy curative effect dynamic evaluation, and the method utilizes a micro-fluidic chip to carry out electrical impedance detection and physical preliminary screening on cells in a whole blood sample. And performing anti-reclosing time sequence verification on the screened interstitial circulating tumor cells, triggering transient flow resistance regulation and control when conditions are met, and opening a lateral shunt channel to reduce the flow velocity of a main flow channel. And meanwhile, the adaptive optical imaging unit is synchronously switched to a long integral time mode to collect a fluorescence image. And finally, the data calculation terminal calculates an immunotherapy curative effect evaluation index in combination with cell counting, an omission compensation item and PD-L1 expression intensity. Through synchronous linkage of the flow velocity and exposure, the problem of weak fluorescence signal detection under high-speed flow is solved, and the evaluation accuracy is improved.

The invention discloses a circulating tumor cell physicochemical screen and application thereof. The circulating tumor cell physicochemical screen comprises a body, a reaction tank is arranged in the body, at least two liquid inlets are formed in one end of the reaction tank, a liquid outlet is formed in the other end of the reaction tank, a plurality of reaction areas are arranged between the liquid inlets and the liquid outlet, and a plurality of micro-columns are arranged in each reaction area; along the direction from the liquid inlet to the liquid outlet, the distance between the micro-columns in each reaction area is gradually reduced. The circulating tumor cells are sorted in a mode of combining physical blocking and chemical adsorption, the distance between the microcolumns in each reaction area is gradually reduced, screening of the CTC cells is facilitated, further observation and judgment are also facilitated, compatibility to various coloring agents is good, and the application range is wide.

The utility model discloses a circulating tumor cell physicochemical sieve which comprises a body, a reaction tank is arranged in the body, one end of the reaction tank is provided with at least two liquid inlets, the other end of the reaction tank is provided with a liquid outlet, a plurality of reaction areas are arranged between the liquid inlets and the liquid outlet, and the reaction areas are arranged along the direction from the liquid inlets to the liquid outlet. And the width of each reaction area is gradually reduced. According to the circulating tumor cell sorting device, circulating tumor cells are sorted in a mode of combining physical blocking and chemical adsorption, the width of each reaction area is gradually reduced, the effect of step-by-step separation and interception can be achieved, the situation that the observation view is blurred due to blockage and accumulation is avoided, screening of the CTC cells is facilitated, and further observation and judgment are also facilitated.

This invention belongs to the field of biomedical technology and provides a high-throughput drug screening system and its usage method for enriching circulating tumor cells (CTCs) in liver cancer. It includes an information processing and control unit; a diseased liver perfusion device comprising an organ chamber containing injection fluid at the bottom, an arterial supply line, an arterial end sensor, an arterial end clamp valve, at least two membrane lungs, a portal vein supply line, a portal vein end clamp valve, and a portal vein end sensor; a CTC enrichment device, which uses erythrocyte lysis to remove erythrocytes and immunomagnetic bead labeling to remove leukocytes, enabling the processing of large volumes of perfusion fluid in a single step to obtain enriched circulating tumor cells; a high-throughput drug screen; and phototubes, arterial end sensors, and portal vein end sensors, each electrically connected to the information processing and control unit. Compared with existing technologies, the high-throughput drug screening system and its usage method of this invention have the advantages of establishing a room-temperature mechanically perfused ex vivo liver cancer model, realizing the processing of large volumes of liquid specimens, and providing automated high-throughput drug screening functionality.

Methods for enriching disseminated tumor cells (DTCs) or circulating tumor cells (CTCs) from a biological sample are provided. Methods for identifying disseminated tumor cells (DTCs) or circulating tumor cells (CTCs) or identifying a tumor type or tissue of origin of a tumor are also provided. Methods of identifying a druggable cancer target and a cancer drug against the target are provided.

The invention discloses a method for capturing, identifying and culturing circulating tumor cells of sarcoma in vitro. The method comprises a step of filtering a peripheral blood sample of a sarcoma patient by using a flexible microfiltration membrane, and cells obtained by filtration can be directly subjected to multiplication culture on the filtration membrane. Immunomagnetic beads of a monoclonal antibody combination group coupled with the anti-human leukocyte surface antigen CD45 can be used for negative enrichment and then multiplication culture is carried out in a cell culture bottle, and the captured or cultured cells can be subjected to subtype identification, characterization and counting by adopting an iFISH technology. It is found for the first time that the flexible microfiltration membrane can be used for capturing and culturing the sarcoma circulating tumor cells, and the flexible microfiltration membrane has no toxic effect on the sarcoma circulating tumor cells. The invention not only provides an effective means for real-time monitoring of relapse and metastasis processes of sarcoma patients and chemotherapy drug resistance monitoring, but also can perform drug sensitivity experimental verification on CTC after multiplication culture, and provides a powerful weapon for accurate diagnosis and treatment of sarcoma.

The utility model provides a multifunctional push-pull rod and a circulating tumor cell screening and separating device, which belong to the technical field of medical detection.The multifunctional push-pull rod comprises a push-pull rod body and an electromagnet, one end of the push-pull rod body is a connecting end, and the electromagnet is mounted at the connecting end; the electromagnet is set to generate a magnetic field when powered on and remove the magnetic field when powered off; the device has the advantages that the electromagnet and the push-pull rod are combined together, so that the whole device is more compact, the function integration level is improved, the on-off of a magnetic field is achieved through the power-on / power-off control of the electromagnet, enrichment and cleaning operation are facilitated, and the electromagnet is located at the connecting end of the push-pull rod body, so that the cleaning efficiency is improved. Therefore, the electromagnet can be located on the center line of the mixing chamber, so that an external magnetic field is uniformly distributed in the mixing chamber, and the enrichment effect of CTCs is improved.

The utility model discloses a circulating tumor cell physicochemical screen which comprises a body, a reaction tank is arranged in the body, one end of the reaction tank is provided with at least two liquid inlets, the other end of the reaction tank is provided with a liquid outlet, and a fluid channel is arranged between the liquid inlets and the liquid outlet. At least two reaction areas are arranged in the fluid channel, and a plurality of micro-columns are arranged in each reaction area; a connecting area is arranged between every two adjacent reaction areas, and no micro-column is arranged in each connecting area. Along the direction from the liquid inlet to the liquid outlet, the distance between the micro-columns in each reaction area is gradually reduced. According to the circulating tumor cell sorting device, circulating tumor cells are sorted in a mode of combining physical blocking and chemical adsorption, blocking pressure is reduced due to the arrangement of the connecting area, fluid can pass through smoothly, and blocking is avoided; the distance between the micro-columns in each reaction area is gradually reduced, so that the separation and screening of the CTC cells are facilitated.

The invention belongs to the technical field of biological detection, and particularly relates to a multichannel circulating epithelial cell separation and enrichment device based on microfluidic immunocapture and application. The device comprises a fluid injection unit, a switching valve, a three-layer structure chip body, a reagent bearing module, a bar code scanning module, a temperature control module and an exhaust air cooling system, wherein a multi-channel micro-fluidic structure, an interface micro-column regulation and control region and a three-dimensional structure enrichment cavity are integrated in the chip body. A switching valve is controlled to automatically switch a sample injection channel through a dynamic scoring algorithm, and precise capture of target cells in a multi-channel enrichment cavity is realized by combining microstructure regulation and control and a temperature difference induction mechanism. The device has the advantages of high capture efficiency, low cell damage, large flux and high intelligent degree, and is suitable for enrichment detection of rare cells such as circulating tumor cells.

The invention discloses a renal cell carcinoma circulating tumor cell culture medium as well as a culture method and application thereof. The invention belongs to the field of biological medicine, and particularly relates to a renal cell carcinoma circulating tumor cell culture medium and a culture method and application. The invention provides a composition for culturing circulating tumor cells, the composition contains a cell culture medium and a biological scaffold, the cell culture medium contains a reagent named as RFGI, and the RFGI contains fetal calf serum, insulin, glucose and R-spondin-1 protein. Enriching the isolated circulating tumor cells through density gradient centrifugation; the composition is used for culturing the enriched circulating tumor cells to obtain the circulating tumor cells, so that the survival rate of the circulating tumor cells is effectively improved, and the composition has huge potential and application prospects for subsequent drug screening.

The invention relates to the technical field of biological medicine, and particularly discloses application of an ICAM1 inhibitor in preparation of a nasopharynx cancer resistant circulating tumor cell cluster preparation. According to the application, expression of a shRNA silent gene ICAM1 is utilized, in-vitro experiments prove that proliferation, migration and invasion ability of nasopharyngeal carcinoma cells can be remarkably reduced by inhibiting the ICAM1, formation of circulating tumor cell clusters and angiogenesis are inhibited, after the nasopharyngeal carcinoma cells are treated by cis-platinum drugs, the activity of ICAM1 silent group cells is remarkably reduced, and the activity of the ICAM1 silent group cells is remarkably reduced. Experimental results show that a relatively low ICAM1 expression level is related to relatively high chemotherapeutic drug sensitivity. The ICAM1 inhibitor shICAM1 provided by the invention has important application value in preparation of a preparation for specifically targeting nasopharyngeal carcinoma circulating tumor cell clusters, and a brand new treatment scheme is provided for solving the problem of nasopharyngeal carcinoma distant metastasis.

The utility model relates to the technical field of tumor cell metastasis models, in particular to a breast cancer circulating tumor cell remote metastasis model which comprises a base and a fixing rod, the fixing rod is fixedly connected to the base, the fixing rod is fixedly connected with a brain metastasis model, a placing component is installed on the brain metastasis model, and the placing component is fixedly connected with the brain metastasis model. The placing part comprises an inclined plate, a fixed plate, a movable plate, a pressed plate, a straight spring and a collecting box, the inclined plate is fixedly connected in the brain metastasis model, the clamping plate clamps small balls with different colors through the matching effect of the extrusion rod, the movable plate, the collecting box and other parts, the small balls can be taken out by pulling the clamping plate, and the small balls can be conveniently taken out. And residual water is left in the collecting box, so that the small balls can be quickly taken out, meanwhile, no water drips on the outer surface of the model, the situation that the transparency of the model is affected by water marks generated on the outer surface of the model due to water dripping is avoided, and the transparency of the organ model and the cleanliness of a placement environment are guaranteed.

Disclosed are methods for detecting and treating cancers (such as carcinoma, leukemia, lung cancer, colon cancer, central nervous system (CNS) cancer, melanoma, ovarian cancer, renal cancer, oral cancer, prostate cancer and / or breast cancer). The method may include receiving a sample from a patient (such as a sample from a biopsy, a sample from an extracellular vesicle, or a sample from a circulating tumor cell). The method may include determining whether a LY6C protein or a phosphorylated ANXA2 protein is detected in the sample. The method may include treating a patient for cancer by targeting the LY6C protein when the LY6C protein is detected (e.g., using photoimmunotherapy (PIT) or near infrared photoimmunotherapy (NIR-PIT)). or treating a patient for cancer by targeting an ANXA2 protein (e.g, using inhibitors, decoys, etc.) when the phosphorylated ANXA2 protein is detected.

To provide safe and effective compositions and methods for enhancing the efficacy and / or reducing the side effects of cancer chemotherapy and increasing the sensitivity of chemotherapy-resistant cancer cells.SOLUTION: Nutritional supplements comprising fish oil and selenium have been found to provide various activities that are beneficial in treating cancer and related conditions. The supplement provides a synergistic effect in reducing cancer cell growth when used in combination with a chemotherapeutic agent and can reduce growth in drug resistant cancer cells when used in combination with a chemotherapeutic agent to which the cells are resistant. Effects in reducing angiogenesis, reducing metastasis, decreasing the number of circulating cancer cells, and altering AXL signaling have also been found. The use of the supplement was found to reduce the wasting associated with cachexia and decrease circulating cytokines associated with inflammation. The overall effect was found to prolong survival in a clinical study.SELECTED DRAWING: Figure 1