The invention discloses an immunofluorescent staining and interpretation method for circulating tumor cells. The method comprises the following steps: S10, performing centrifugal treatment on a blood sample diluted with a phosphoric acid buffer solution, and removing erythrocyte and leukocyte in the blood sample; S20, moistening the treated blood sample with the phosphoric acid buffer solution, adding a stationary liquid for immobilizing cells in the blood sample, adding a permeabilization liquid for permeabilization, and further adding a confining liquid for sufficient confining; and S30, dripping dye liquids of anti-pan-CK-FITC, anti-CD45-AF59 and anti-Vimentin-FITC into the treated blood sample at a dark place, performing light shielded incubation, removing the dye liquids, adding a nucleic acid fluorescent dye to continue incubation. The interpretation method comprises steps of scanning observation and result interpretation. Compared with the prior art, the dyeing method disclosed by the invention has the advantages of being simple and convenient to operate, easy to popularize and the like, and the interpretation method has the advantages of being accurate interpretation and the like.