393 results about "Microvessel" patented technology

In an aspect, the present invention uses projection micro stereolithography to generate three-dimensional microvessel networks that are capable of supporting and fostering growth of a cell population. For example, provided is a method of making a microvascularized bioreactor via layer-by-layer polymerization of a photocurable liquid composition with repeated patterns of illumination, wherein each layer corresponds to a layer of the desired microvessel network. The plurality of layers are assembled to make a microvascular network. Support structures having different etch rates than the structures that make up the network provides access to manufacturing arbitrary geometries that cannot be made by conventional methods. A cell population is introduced to the external wall of the network to obtain a microvascularized bioreactor. Provided are various methods and related bioreactors, wherein the network wall has a permeability to a biological material that varies within and along the network.

Microvessel networks are produced in vitro from tissue-engineered parent vessels sprouting into a supporting matrix, as for example gels, of extracellular matrix proteins. The microvessel systems are integrated into devices that allow for controlled perfusion with fluids. The vessels may include cells from one cell type, for example, endothelial cells, or from combinations of two or more cell types.

The invention discloses a thrombus aspiration catheter. The catheter comprises an aspiration tube, wherein the aspiration tube comprises a tube socket which is connected with a catheter; a bushing is movably sleeved on the outer wall of the aspiration tube, and comprises a Y-shaped connector, a dual-lumen catheter and a balloon; the Y-shaped connector is connected with the dual-lumen catheter, and the balloon is arranged on the outer wall of the distal end of the dual-lumen catheter; an interference metal wire can be inserted into the aspiration tube; and the distal end of the interference metal wire can extend outside the distal end of the aspiration tube. The bushing is movably arranged outside the aspiration tube, and the balloon is not limited when the aspiration tube moves, so that the thrombus aspiration catheter can quickly eliminate thrombi dispersed in a wide area of a blood vessel, and treats embolism of coronary peripheral microvessels; and when large or high-viscosity thrombus is encountered, the thrombus can be scattered by utilizing the interference metal wire and aspired, so that the thrombus aspiration catheter can treat the thrombus which is difficult to aspire.

MVO is treated by introducing injectate into blood vessels affected by MVO at precise flow rates, while blocking retrograde flow, such that the natural pumping of the heart aids in forcing the injectate into the affected microvessels. Monitoring pressure distal of an occlusion balloon is used to determine treatment effectiveness and heart health.

Clinical performance of currently available human skin equivalents is limited by failure to develop perfusion. To address this problem we have developed a method of endothelial cell transplantation that promotes vascularization of human skin equivalents in vivo. Living skin equivalents were constructed by sequentially seeding the apical and basal surfaces of acellular dermis with cultured human keratinocytes and Bcl-2 transduced HUVEC or umbilical cord cells sequentially. After orthotopic implantation of grafts comprising cultured human keratinocytes and Bcl-2 transduced HUVEC cells onto mice, the grafts displayed both a differentiated human epidermis and perfusion through the HUVEC-lined microvessels. These vessels, which showed evidence of progressive maturation, accelerated the rate of graft vascularization. Successful transplantation of such vascularized human skin equivalents should enhance clinical utility, especially in recipients with impaired angiogenesis.

The present invention provides novel compositions comprising multipotent cells or microvascular tissue, wherein the cells or tissue has been sterilized and/or treated to inactivated virases, and related methods of using these compositions to treat or prevent tissue injury or disease in an allogeneic subject.

The invention relates to the technical field of medical technology assistance, in particular to an artificial intelligence diagnosis method for gastric cancer under a narrow-band imaging amplificationgastroscope, which comprises the following steps: S1, constructing a mini-UNet neural network model; S2, constructing a UNet + + image segmentation neural network model, and obtaining an area ratio Rabnormal of an image feature difference region; S3, adopting a generative adversarial network (GAN) technology to obtain a microvascular morphological diagram and a microstructure morphological diagram of the feature abnormal region; S4, identifying the microvessel form dissimilarity degree and the microstructure form dissimilarity degree in the microvessel form diagram and the microstructure formdiagram by the neural network model ResNet50; and S5, carrying out identification and judgment by using the random forest model obtained by training to obtain final judgment of cancer or non-cancer,and identifying the canceration position range of the image, which is judged to be cancer, as the identified image feature difference region Pabnormal. The sensitivity and specificity of the kit to cancer and non-cancer recognition reach about 93.4% and 90.7% respectively, a clinician can be effectively assisted in discriminating and diagnosing cancer and non-cancer, and a canceration position range is given.

The invention relates to the technical field of medical image processing, in particular to a gastric early cancer auxiliary diagnosis method based on a deep learning multi-model fusion technology, which comprises the following steps: S1, constructing multiple models; s2, collecting gastroscope images, forming continuous serialized image frames, identifying the light source mode of the current image frame by utilizing the image classification model 1, entering the step S3 to mark the position of a focus by the target detection model 2 when the current image frame is identified as a white lightmode, and marking the high-risk focus by utilizing the image classification model 3; and when the image frame is identified as the dyeing amplification mode, entering the step S4 in which a segment model group can extract the boundary range, the microvessel form and the micro-tissue structure feature map in the image frame in real time, and outputting whether canceration occurs or not, the credibility and the differentiation type by the decision-making model 7. A plurality of deep learning models are constructed according to different tasks, a parallel cascade model fusion technology is adopted, and a full-process intelligent auxiliary diagnosis function is provided in the stomach early cancer screening process of endoscopists.

A method for creating networks of perfusable microvessels in vitro. A mandrel is drawn through a matrix to form a channel through the matrix. Cells are injected into the channel. The matrix is incubated to allow the cells to attach inside the channel. The channel is perfused to remove unattached cells to create a parent vessel, where the parent vessel includes a perfusable hollow channel lined with cells in the matrix. The parent vessel is induced to create sprouts into the surrounding matrix gel so as to form a microvessel network. The microvessel network is subjected to luminal perfusion through the parent vessel.

A method of reading a plurality of encoded microvessels used in an assay for biological or chemical analysis. The method can include providing a plurality of encoded microvessels. The microvessels can include a respective microbody and a reservoir core configured to hold a substance in the reservoir core. The microbody can include a material that surrounds the reservoir core and facilitates detection of a characteristic of the substance within the reservoir core. Optionally, the material can be transparent so as to facilitate detection of an optical characteristic of a substance within the reservoir core. The microbody can include an identifiable code associated with the substance. The method can also include determining the corresponding codes of the microvessels.

The invention discloses a device and method for detecting human body microcirculation by means of orthogonal polarization spectral imaging. The device comprises a first point light source, a first 805-nm narrow-band interference filter, a first focusing lens, a second point light source, a first 548-nm narrow-band interference filter, a second focusing lens, a light composition lens, an isolation lens, a polarization splitting prism, a microscope objective, a polarization analyzer, a beam splitter, a second 548-nm narrow-band interference filter, a second 805-nm narrow-band interference filter, a first CCD camera, a second CCD camera and a computer. Parallel light with the wavelength being 805 nm and 548 nm is irradiated on the light composition lens, perpendicularly reflected by the isolation lens and the polarization splitting prism, and focused on detected tissue on a finger rest through the microscope objective, light reflected from the interior of the detected tissue passes through the polarization analyzer and then is irradiated on the beam splitter, the first and second CCD cameras shoot images which are processed by the computer, and then clear microvessel images can be obtained. The influence of movement of a target object on the detected tissue on imaging can be eliminated, and the imaging quality can be improved.

The invention discloses a hydrogel containing a heparin-poloxamer compound for anastomosis of blood vessel and a preparation method thereof. The concentration of the heparin-poloxamer compound in the hydrogel is 10-40% (W/V). The hydrogel is pre-sealed in a sterile injector or ampoule, or the hydrogel is freeze-dried into freeze-dried powder and poured into a penicillin bottle, and before use, the freeze-dried powder is dissolved in injection water to form hydrogel. The hydrogel containing the heparin-poloxamer compound disclosed by the invention is applied to the micro-anastomosis of blood vessel in combination with a medical adhesive, the assistance of soluble endovascular stent or suture is not needed, the closeness of the anastomotic stoma is enhanced, the phenomenon of leakage is avoided, and the risk of trauma and thrombosis during the anastomosis of blood vessel is reduced.

The present invention relates to the use of an hydroxylated stilbene, in particular resveratrol, in the manufacture of a neutraceutical composition for increasing the mi- crovascular plasticity and/or microvessel density, and/or decreasing the microvessel abnormalities in the brain, in particular in the hippocampus of a mammal, in particular for the treatment of age- and condition-related decline in brain neuronal function and/or cognitive functioning in a mammal. In particular, the condition is selected from the group of Alzheimer's Disease, dementia, depression, sleep disorders, impaired memory function, psychoses, Parkinson's disease, Huntington's chorea, epilepsy, schizophrenia, paranoia, ADHD and anxiety.

A method for creating networks of perfusable microvessels in vitro. Cells including cell types capable of sprouting are seeded 1300 into a channel in a matrix at to activate competency 1304 of the cells for sprouting as microvessels based on the seeding density. The matrix channel is perfused with medium to allow parent vessels to form and for viability 1324. The parent vessels and matrix are incubated and perfused to provide for sprouting of microvessels from parent vessels into the surrounding matrix 1328. The sprouting parent vessels are grown until network forms 1332.

Provided is a process for creating a 3D metabolically active microtissue perfused with living microvessels which have a direct fluidic connection with neighboring microfluidic channels. The process comprises preparing a template comprising a plurality of channels, and creating a network within said channels, said network comprising microfluidic channels, metabolically active living microvessels, and microtissues. The microvessels can sprout from said microvessels and/or form within the microtissue in response to a stimulus applied from said microfluidic channels or stimulus derived from the said tissues. In another embodiment, a device is provided comprising a supportive structure, one or more microfluidic channels, one or more microtissue compartments, and one or more microvessels, whereby the microvessels connect said microfludic channels and microtissue and perfuse the microtissue to deliver fluid from the microfluidic channels to the microtissues.

The invention relates to the technical field of optical fiber sensor medical devices, in particular to an OCT imaging compound micro probe integrated with optical fiber sensing multi-parameter measuring, and discloses a preparation method of the composite micro probe. The probe comprises a multi-core optical fiber, a side-hole optical fiber, a single-mode optical fiber and a multi-mode optical fiber which are sequentially welded, the multi-mode optical fiber comprises a central axis fiber core and an eccentric fiber core, the central axis fiber core and the fiber core of the single-mode optical fiber are welded at two ends of the fiber core of the side-hole optical fiber, photoetching FBG optical grating is arranged on the eccentric fiber core, and the front end of the eccentric fiber coreand the rear end of a side-hole air cavity on the side-hole optical fiber are aligned to further form an F-P air cavity; the front end of the multi-mode optical fiber is fused with a Bell ball. The structure of the probe is reasonable, sensitive units of FBG grating, F-P air cavity are integrated, a Bell ball focusing lens is fused through the multi-mode optical fiber, the definition of an OCT imaging observing image can be effectively improved, body tissue detecting imaging is conducted while measuring of parameters of temperature, pressure and the like is conducted, the overall structure iscompact, the size is small, and the probe has significant meaning in in vivo microvessel disease diagnosis and the like.

A new indication of vitamin K is disclosed which results in the provision of methods of treatment, both therapeutic and non-therapeutic, as well as functional foods and pharmaceutical and nutraceutical products comprising vitamin K for improving microvascular integrity and capillary structure and function, thus preventing, mitigating, counteracting or curing diseases associated with impaired capillary morphology including the glycocalyx or capillary dysfunction. Preferably, vitamin K is a menaquinone, most preferably selected from the long-chain menaquinones MK-7 through MK-10.

The invention relates to an angiography method applied to optical coherence tomography and an OCT system. Based on the frequency division thought, OCT interference fringes are decomposed into multiple wave number bands, and noise generated when shot tissue moves is lowered. After intensity images obtained after frequency division are acquired, an improved CM method is further adopted, the change degree of the intensity, calculated through the Pearson correlation coefficient, between adjacent continuously scanned cross-sectional images is calculated, and signals of blood vessels are enhanced. In combination with information of points adjacent to detection points, sensitivity to blood vessel detection is improved, sensitivity to eye movement is reduced, and the method is suitable for angiography of biological tissue with high scattering. The method can be used for angiography of anterior segment sclera or irises or other tissue in ophthalmology and can be applied to microvascular imaging of other portions of the human body.

The invention relates to application of a deep learning algorithm in the field of medical image analysis, in particular to a retinal vessel segmentation algorithm fusing W-net and a conditional generative adversarial network. According to the method, the problems of low segmentation sensitivity and insufficient micro blood vessel segmentation are well solved, great progress is made in the aspectsof the parameter utilization rate, the information circulation and the feature analysis ability of the network, complete segmentation of the main blood vessel and fine segmentation of the micro bloodvessel are facilitated, the blood vessel intersection is not prone to breakage, and a focus and an optic disc are not prone to being segmented into the blood vessel by mistake. According to the invention, a plurality of network models are fused under the condition of low complexity; the whole segmentation performance on a DRIVE data set is excellent, the sensitivity and accuracy of the method are87.18% and 96.95% respectively, the ROC curve value reaches 98.42%, the method can be used for computer-aided diagnosis in the medical field, and rapid and automatic retinal vessel segmentation is achieved.

The invention provides a composition, preparation and an appliance of a neural unit spherical support, wherein a large number of channels are distributed in the neural unit spherical support, and is of a space structure which radiates from the center to the periphery, a center hole is communicated with all channels, the diameter of the neural unit spherical support is 500-2000 Num, the diameter of the channels in the inner portion of the neural unit spherical support is 20-100 Num, and stem cells and vascular endothelial cells are arranged in the channels. The neural unit spherical support can achieve the multiple purposes of effective filling of nerve defect areas and rapid reconfiguration of local neural network and microvessel and the like after being transplanted, thereby achieving the effect that the functions of the nerve defect areas rapidly recover.

The invention discloses an implanted artificial liver comprising a polyglycolic acid or polylactic-co-glycolic acid sleeve, more than one three-dimensional porous scaffold, and polyglycolic acid or polylactic-co-glycolic acid tube seals; the three-dimensional porous scaffolds are mounted inside the polyglycolic acid or polylactic-co-glycolic acid sleeve; and polyglycolic acid or polylactic-co-glycolic acid tubes are used for sealing two ends of the polyglycolic acid or polylactic-co-glycolic acid sleeve. The invention has the beneficial effects of maximally simulating the structure of a hepatic lobule, developing implanted stem cells into liver cells through directional differentiation, and inducing the regeneration of microvessels of a tissue engineering liver. Meanwhile, the exterior of the three-dimensional porous scaffolds is wrapped in the highly biocompatible biomaterial sleeve which serves to simulate the structure of a liver capsule; and small lacunas are formed between the sleeve and the scaffolds to allow blood to circulate through tissue engineering brackets, thereby facilitating the regeneration of vessels of the engineering liver in an envelope, and ultimately forming an artificial liver to replace the damaged liver of a parasitifer.

The invention relates to a non-fluorescent eye fundus image based automatic segmentation method for retinal blood vessels. The method comprises following steps: step one, an image is preprocessed, blood vessel characteristics are enhanced, and background noise is weakened; step two, morphological characteristics of the retinal image are extracted with a multi-scale linear operator, and a refined blood vessel framework is segmented with two-dimensional Gabor wavelet transform; step three, after the refined blood vessel framework is segmented, the image is subjected to path morphology filtering, the maximum path length is combined with geometrical characteristics of a blood vessel region, and an adjacent map is constructed; step four, a binaryzation process is performed with regional connectivity analysis and a hysteresis threshold technology for auxiliary segmentation of microvessels, and the segmentation is finished. Compared with the prior art, the method has the advantages as follows: the blood vessel location accuracy is higher, the adhesion phenomena are reduced, and a retinal blood vessel network of the eye fundus image is effectively segmented.