27 results about "Acellular Dermis" patented technology

The present invention relates to a method for preparing an acellular dermal matrix derived from a human body, in which the acellular dermal matrix is washed for the second time with an alcohol-based degreasing solution, thereby not only having excellent safety against cytotoxicity, but also being able to effectively remove residual fat.

Improved allografts having controlled and consistent properties and useful for soft tissue repair, including breast reconstruction, and other surgical procedures, are disclosed. In one embodiment, an improved acellular dermal matrix (ADM) is produced from a skin sample, where the ADM consists essentially of a single dermal tissue type (e.g., papillary or reticular) and comprises a collagen matrix having substantially uniform density and porosity. In another embodiment, two ADMs are produced from the same dermal tissue type (e.g., papillary or reticular) of the same skin sample, each of which consists essentially of reticular dermis, and comprises a collagen matrix having substantially uniform density and porosity. All such improved ADMs are suitable for use in breast reconstruction and other tissue repair and modification surgery procedures.

The present invention relates to a gelatin-coated acellular dermal tissue and a use thereof. More specifically, the present invention relates to: an acellular dermal tissue in which gelatin derived from human skin tissue is crosslinked on the surface of the acellular dermal tissue; and a use thereof for treating skin damage. The gelatin-coated acellular dermal tissue according to the present invention has predetermined physical properties so as to provide ease of handling, and also eliminates toxicity and transplant rejection responses, and thus can be effectively used for treating patients with skin tissue damage.

The present invention relates to: a fish-derived acellular dermal matrix having improved tissue-to-tissue adhesion problems while ensuring biocompatibility by removing cellular components from fish-derived by-products; and a preparation method therefor.

The invention discloses a double-layer GBR membrane for promoting soft and hard tissue repair and a preparation method and application thereof, and is applied to the field of medical materials. The double-layer GBR membrane mainly comprises an acellular dermal matrix obtained by decellularizing fetal calfskin and a piezoelectric layer prepared by electrostatic spinning of poly-L-lactic acid and chitosan, and the acellular dermal matrix and the piezoelectric layer are further combined through a polydopamine coating prepared on the surface of the piezoelectric layer. During application, the acellular dermal matrix side faces the soft tissue side, and the piezoelectric layer faces the bone tissue side, so that the acellular dermal matrix skin side which is compact in structure is in contact with the soft tissue to play a barrier role, and the relatively loose piezoelectric layer is in contact with bone defects to play a role in guiding osteoblasts to adhere; functionally, soft tissue repair is promoted through the angiogenesis promoting effect of the acellular dermal matrix and the similarity of the tissue of the acellular dermal matrix, and bone regeneration of a bone defect area is promoted through the piezoelectric osteogenesis and angiogenesis effects of the piezoelectric layer.

The invention provides a composition for delaying cell senescence as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: loading tea polyphenol on a dog bone marrow mesenchymal stem cell exosome, and modifying the surface of the dog bone marrow mesenchymal stem cell exosome with arginine nonamer to prepare a modified tea polyphenol-loaded exosome; the preparation method comprises the following steps of: modifying cholesterol by using arginine nonamer, and preparing lipidosome by using the cholesterol modified by the arginine nonamer, DSPE-PEG2000, phospholipid embedded resveratrol and epigallotein gallate; polysaccharide is extracted from rhizoma polygonati, fructus lycii and ginseng, and rhizoma polygonati / fructus lycii / ginseng polysaccharide is prepared; the preparation method comprises the following steps: mixing an acellular dermal matrix with polygonatum sibiricum / medlar / ginseng polysaccharide, adding lipidosome and a modified tea polyphenol-loaded exosome, co-incubating, and freeze-drying to prepare the composition for delaying cell aging, the composition has a better drug embedding effect, can improve the stability and transdermal absorption effect of the drug, has better antioxidant, antibacterial and anti-inflammatory effects, and can be used for preparing the medicine for delaying cell aging. The effect of delaying skin cell senescence can be better achieved through multiple ways, and safety and degradability are achieved.

The application discloses a kind of for hernia repair containing acellular dermis biological patch and preparation method thereof, belong to biomedical material field, raw material includes acellular porcine dermis layer matrix 90-110 parts, genipin 0.1-0.2 parts, EDC 0.8-2.5 parts, NHS 0.3-1 part, carboxymethyl chitosan 3-8 parts, sodium hyaluronate 1.5-4 parts, chondroitin sulfate 0.5-2 parts, glycerol 2-5 parts, modified zinc tannate nanocomposite 3-12 parts, effectively solve the problems that existing hernia repair patch anti-adhesion and tissue ingrowth is difficult to coordinate, support strength and degradation rate are not matched, foreign body exists in human tissue for a long time and leads to inflammation, by introducing anti-adhesion coating containing zinc tannate coordination nanocomposite and asymmetric double-sided structure, the patch realizes abdominal cavity side long-acting anti-adhesion, abdominal wall side rapid tissue integration and controllable degradation and mechanical support under low immunogenicity.

A soft tissue filling composition according to one aspect of the present invention has a high collagen content and a low lipid content, and thus has high biocompatibility and moldability without causing allergic reactions and inflammation. In addition, the composition has a high complex viscosity and storage modulus, and thus can perform an excellent supporting function, maintains homogeneous properties, and can be injected with low injection force by using a standard syringe. Accordingly, the soft tissue filling composition according to one aspect of the present invention can be used to restore the volume of defective or recessed soft tissues, for example, breasts, and can be used for alleviating skin curvature, cosmetic fillers, or supplementing moisture sensation.

This invention discloses a method for preparing acellular dermal matrix, belonging to the field of tissue engineering technology. The preparation method includes: selecting pelts from traceable animals aged one to three months, purifying them through steps such as soaking, fleshing, defatting, hair removal, liming, deliming, and softening in a tanning process to obtain acellular dermal matrix. The acellular dermal matrix obtained by this invention removes antigenic components such as cells, hair follicles, and extracellular proteins during the preparation process, minimizing its immunogenicity while preserving the collagen fiber structure in its extracellular matrix. Simultaneously, the preparation method of this invention moderately loosens the collagen fiber bundles. This process not only effectively alleviates the entanglement between fiber bundles but also successfully increases the exposure of hydrophilic groups, giving the acellular dermal matrix excellent hydrophilicity, which is more conducive to cell proliferation and migration.

The invention discloses a preparation process of an efficient acellular dermal matrix, which comprises the following steps: pretreating animal fetal skin to obtain dermal tissue, sequentially carrying out specific acellular treatment and degreasing treatment processes, and subsequently carrying out crosslinking, freeze-drying and sterilization to obtain the acellular dermal matrix. According to the preparation process of the acellular dermal matrix, impurities and immunogenic substances can be effectively removed, and meanwhile, the activity of the extracellular matrix is protected to the maximum extent; the obtained acellular dermal matrix product is complete in collagen triple helix structure, low in residues of DNA, alpha-Gal antigens and the like, extremely low in fat content, free of cytotoxicity and high in safety.

The application discloses a preparation process of efficient acellular dermal matrix, and the acellular dermal matrix is prepared through the following steps: obtaining dermal tissue through pretreatment of animal fetal skin, and then performing specific acellular treatment and defatting treatment processes, and finally performing crosslinking, freeze-drying and sterilization. The acellular dermal matrix preparation process can effectively remove impurities and immunogenic substances, and at the same time, the activity of extracellular matrix is maximally protected. The obtained acellular dermal matrix product has complete triple-helix structure of collagen, low residues of DNA and alpha-Gal antigen, extremely low fat content, no cytotoxicity and high safety.

The embodiment of the application discloses a preparation method of humanized extracellular matrix, and belongs to the technical field of biomedical materials and tissue engineering. The method comprises the following steps: preparing human decellularized dermal matrix; preparing a concentrated solution of a condition medium rich in human active factors; constructing RGD-PLGA / BMP-2 nanocomposites; loading the concentrated solution of the condition medium into the human decellularized dermal matrix through vacuum negative pressure perfusion, and then anchoring the RGD-PLGA / BMP-2 nanocomposites, and cross-linking them twice through genipin to obtain the humanized extracellular matrix. Through the four-element strategy of decellularized matrix-active factor-nano-sustained release-two-stage cross-linking, the unity of low immunogenicity, high biological activity and spatiotemporal controllable release function is realized, and the humanized extracellular matrix can be applied to the fields of tissue engineering scaffolds, wound repair and bone defect filling.

The invention discloses a metabolizable and anisotropic tensile hernia patch and a preparation method thereof. The material is prepared from the following raw material components in parts by weight: 10-15 parts of a high polymer material, 80-180 parts of a solvent, 1-3 parts of a piezoelectric material, 0.05-0.15 part of an acellular dermal matrix and 8-12 parts of an acetic acid solution. Through high-speed electrostatic spinning and angle layering design, the mechanical property of the patch can be accurately regulated and controlled, and the anisotropy of an abdominal wall muscle layer is simulated. The multilayer staggered structure is similar to the effect of plywood, can effectively disperse and bear stress in multiple directions, prevents the patch from shrinking or bulging, and provides more stable and durable mechanical support.

The utility model discloses a folding type decellularized dermal skin taking device, and belongs to the field of skin taking devices. Mainly comprises a skin taking device, the skin taking device comprises a knife assembly, the knife assembly comprises two knife handles, a cavity is formed between the two knife handles, the knife handles are used for being held and taken by workers, knife bodies are movably installed at the ends of the knife handles, the knife bodies are used for making contact with genuine leather, and connecting rods are movably installed on supporting strips. According to the folding type decellularized dermis taking device, the multiple sets of tooth openings are formed in the end of the knife body, the multiple sets of tooth openings can be in contact fit with the clamping block, so that the knife body can be suitable for adjustment at different angles, and the operation is convenient. And therefore, the knife body can be located at different positions on the dermis, the situation that a worker needs to frequently rotate the wrist for operation is avoided, and the overall using effect of the device is improved.

The invention provides a porcine acellular dermal matrix for hip joint labial glenoid reconstruction as well as a preparation method and application of the porcine acellular dermal matrix, and belongs to the technical field of biomedical materials. Cleaning and incubating the natural pig dermis tissue; then treating by using a solution containing EDTA-pancreatin and a solution containing a surfactant in sequence; and finally, washing to obtain the porcine acellular dermal matrix. According to the invention, the porcine acellular dermal matrix is treated by a specific process and is used as a heterogeneous hip joint labial glenoid reconstruction material, and good biocompatibility and tissue guiding function are shown. Meanwhile, the preparation method is simple, the raw material source is wide, and the problem of graft source limitation in the existing hip joint laparolabial glenoid reconstruction operation is solved.

The invention provides an artificial dermis material and a preparation method thereof.The preparation method comprises the following steps that pig dermis tissue is treated with high-concentration alkali liquor, and porous dermis tissue is obtained; sequentially performing degreasing treatment and drying treatment on the porous dermal tissue to obtain a porous acellular dermal matrix; adding the micronized pig dermal matrix, gelatin and honey into water, and uniformly mixing to obtain a composite adhesive liquid; and laying the composite adhesive liquid on one side of a porous acellular dermal matrix, and sequentially carrying out freezing fixation and vacuum drying to obtain the artificial dermal material. Therefore, the obtained artificial dermis material is closer to a physiological structure, the upper layer of dermis can provide mechanical support and ventilation functions, and the lower layer of dermis can quickly absorb tissue penetrating fluid to form a weakly acidic gel protective layer by utilizing high osmotic pressure of honey at the initial stage of wound formation.

The invention belongs to the technical field of biological materials, and particularly relates to an acellular dermal matrix as well as a preparation method and application thereof. The preparation method comprises the following steps: placing the discarded megalovus tissue cut off from a patient in a cold chain preserving fluid, and repairing epidermis and a fat layer under a sterile condition to obtain a pretreated tissue; sequentially carrying out decellularization and depigmentation treatment on the pretreated megalovus tissues to obtain depigmented megalovus tissues; finally, stabilizing treatment and sterilization are carried out, and the acellular dermal matrix is prepared. According to the preparation method disclosed by the invention, the autologous megalovus tissue is taken as a donor, melanin and nevus cells in the tissue can be thoroughly removed, and the risks of megalovus recurrence and melanin deposition are effectively reduced; meanwhile, the natural structure and biomechanical properties of the dermal matrix can be completely reserved, waste is utilized, and reliable biomechanical support is provided for subsequent replanting application of the acellular dermal matrix.

The invention relates to immunoregulation hydrogel with antibacterial and antioxidant characteristics and a preparation method of the immunoregulation hydrogel. The immunoregulation hydrogel is prepared from nanoparticles self-assembled by procyanidine and chlorhexidine acetate, methacrylated gelatin and a porcine acellular dermal matrix. The hydrogel disclosed by the invention has good biocompatibility, self-healing capability and mechanical property, has excellent antibacterial and antioxidant properties, can effectively regulate immunity and promote wound healing, shows remarkable effects in all aspects, and has a good application prospect.

This invention belongs to the field of biomedical materials and tissue engineering technology, and provides a decellularized matrix composite hydrogel dressing, its preparation method, and its application. The decellularized matrix composite hydrogel dressing provided by this invention includes a hydrogel matrix and ginger exosome-like nanoparticles (GELNs) dispersed in the hydrogel matrix; the hydrogel matrix is ​​prepared from raw materials including decellularized dermal matrix and decellularized aortic adventitia matrix. The composite hydrogel dressing of this invention has a three-dimensional porous structure, enabling sustained release of GELNs. It also significantly accelerates the healing process of full-thickness skin defects by downregulating the TLR4 / MyD88 / NF-κB signaling pathway, synergistically regulating macrophage polarization towards the M2 type, and simultaneously promoting angiogenesis and collagen deposition. The dressing has widely available raw materials, good biocompatibility, and broad application prospects in the field of skin wound repair.

The application provides a pig acellular dermal matrix for hip joint labrum reconstruction and a preparation method and application thereof, and belongs to the technical field of biomedical materials. Natural pig dermal tissue is washed and incubated, then is treated with a solution containing EDTA-trypsin and a solution containing a surfactant in sequence, and finally is washed to obtain the pig acellular dermal matrix. The pig acellular dermal matrix is treated by a specific process, is used as a xenogenic hip joint labrum reconstruction material, and exhibits good biocompatibility and tissue guiding function. Meanwhile, the preparation method is simple, raw materials are widely sourced, and the limitation of a graft source in a current hip joint labrum reconstruction surgery is solved.

The application provides a preparation method and application of a double-sided tissue adhesive, which is based on dopamine chemistry and hydrogel coating technology, uses a xenogeneic acellular dermal matrix dressing as a substrate, and polydopamine as a coating. First, a xenogeneic acellular dermal matrix dressing with polydopamine nanoparticles deposited thereon is obtained, anisotropic xenogeneic acellular dermal matrix dressing is prepared through hydrophobic modification, and then a hydrogel coating is assembled to the hydrophilic side of the modified xenogeneic acellular dermal matrix dressing to obtain a double-layer tissue adhesive with asymmetric adhesive properties. The method for preparing the double-layer tissue adhesive has the advantages of reusability, low cost, simple operation and convenience for large-scale production, and the double-sided tissue adhesive prepared has the properties of anti-bursting, asymmetric wettability, asymmetric adhesion and anti-oxidation, can promote tissue repair and resist postoperative adhesion, and can be applied in the preparation of a dressing for promoting tissue repair.

Improved allografts having controlled and consistent properties and useful for soft tissue repair, including breast reconstruction, and other surgical procedures, are disclosed. In one embodiment, an improved acellular dermal matrix (ADM) is produced from a skin sample, where the ADM consists essentially of a single dermal tissue type (e.g., papillary or reticular) and comprises a collagen matrix having substantially uniform density and porosity. In another embodiment, two ADMs are produced from the same dermal tissue type (e.g., papillary or reticular) of the same skin sample, each of which consists essentially of reticular dermis, and comprises a collagen matrix having substantially uniform density and porosity. All such improved ADMs are suitable for use in breast reconstruction and other tissue repair and modification surgery procedures.

There is disclosed a packaged allograft implant configured for implantation in a human recipient. In an embodiment, the packaged allograft implant includes an acellular dermal matrix sheet having a top surface and a bottom surface in opposition to one another, a perimeter surrounding the top surface and the bottom surface, and a thickness extending between the top surface and the bottom surface. The packaged allograft implant further includes a mesh pattern extending across at least a portion of the top surface and the bottom surface of the acellular dermal matrix sheet, the mesh pattern providing through-holes extending between the top surface and the bottom surface of the acellular dermal matrix sheet, and the mesh pattern having a plurality of mesh lines extending in a first direction and a second direction. The first direction and the second direction are orthogonal to one another so as to allow a given amount of stretch in each of the first direction and the second direction. Other embodiments are also disclosed.

The invention provides a high tissue induction type extracellular matrix composite scaffold as well as a preparation method and application thereof, and belongs to the technical field of tissue engineering. The acellular dermal matrix microfibers and the high polymer material braided fabric are compounded for use, and the acellular dermal matrix short fibers with high tissue inductivity endow cell adhesion and tissue growth, so that the material has huge potential in the field of rotator cuff injury repair; molding parameters, freeze-drying parameters and crosslinking process parameters in a preparation process are confirmed through multiple tests, preparation and research, so that the material is endowed with high mechanical property and liquid absorption property of far-reaching supermarket noodle products. The composite scaffold obtained by the invention can be well used for repairing the injury of the rotator cuff, and has a relatively great application prospect.

The invention relates to multifunctional in-situ repair hydrogel based on a modified dermal matrix as well as a preparation method and application of the hydrogel. The hydrogel is formed by crosslinking a first polymer precursor and a second polymer precursor through dynamic covalent bonds of boric acid ester, the first polymer precursor is carboxymethyl chitosan grafted with a phenylboronic acid group and an epsilon-polylysine chain segment; the second polymer precursor is a soluble acellular dermal matrix which is grafted with a 3, 4-dihydroxyphenyl propionic acid group and is subjected to enzymolysis treatment. The hydrogel disclosed by the invention has good biocompatibility, moldability and potential self-healing capability.

The invention relates to a universal biological acellular dermal matrix (ADM) prosthesis for use in body reconstruction processes, comprising: a first layer of ADM of biological origin, arranged at the bottom to delimit a cavity with a bottom edge; a packing material which is arranged in the cavity surrounded by the first ADM layer and consists of ground ADM material having the consistency of a fibrous pulp; and a second layer of ADM of biological origin which is arranged on the upper part and covers the packing material and the top edge of which rests, at its periphery, on the bottom edge of the first layer of ADM, thereby defining a rim; wherein the first layer of ADM and the second layer of ADM are joined by precisely arranging the upper edge of the first layer of ADM on top of the bottom edge of the rim, at the periphery, using a biocompatible glue or using sutures made of resorbable thread; wherein the first layer of ADM, the second layer of ADM and the ADM packing material are not immunogenic; and wherein the ADM comprises collagen fibres, fibronectin, elastin, laminin, glycosaminoglycans and hyaluronic acid. The invention also relates to a method for producing same.

The application provides an injectable microparticulate acellular dermal matrix and a preparation method and application thereof, and belongs to the technical field of preparation of acellular dermal matrixes.The preparation method of the microparticulate acellular dermal matrix comprises the following steps: (1) taking a dermal layer, performing homogenization and defatting treatment, and then performing decellularization treatment; (2) freezing under the condition of liquid nitrogen at-196 DEG C for 1-2 minutes; (3) placing in a cold trap circulating medium, controlling the temperature to be below-50 DEG C, and performing ball milling under the temperature condition by using milling balls with different sizes; (4) after the ball milling is completed, screening the obtained product in step (3), and directly collecting the product in a sterile collection container by using a turbine airflow to form a low-pressure area and storing the product, so that the microparticulate acellular dermal matrix is obtained.The microparticulate acellular dermal matrix obtained by the method has good uniformity, high product quality and high stability, and has good injectability, and can be used for minimally invasive surgical treatment of primary premature ejaculation.