15 results about "Acellular Dermis" patented technology

The present invention relates to a gelatin-coated acellular dermal tissue and a use thereof. More specifically, the present invention relates to: an acellular dermal tissue in which gelatin derived from human skin tissue is crosslinked on the surface of the acellular dermal tissue; and a use thereof for treating skin damage. The gelatin-coated acellular dermal tissue according to the present invention has predetermined physical properties so as to provide ease of handling, and also eliminates toxicity and transplant rejection responses, and thus can be effectively used for treating patients with skin tissue damage.

The present invention relates to: a fish-derived acellular dermal matrix having improved tissue-to-tissue adhesion problems while ensuring biocompatibility by removing cellular components from fish-derived by-products; and a preparation method therefor.

The invention discloses a double-layer GBR membrane for promoting soft and hard tissue repair and a preparation method and application thereof, and is applied to the field of medical materials. The double-layer GBR membrane mainly comprises an acellular dermal matrix obtained by decellularizing fetal calfskin and a piezoelectric layer prepared by electrostatic spinning of poly-L-lactic acid and chitosan, and the acellular dermal matrix and the piezoelectric layer are further combined through a polydopamine coating prepared on the surface of the piezoelectric layer. During application, the acellular dermal matrix side faces the soft tissue side, and the piezoelectric layer faces the bone tissue side, so that the acellular dermal matrix skin side which is compact in structure is in contact with the soft tissue to play a barrier role, and the relatively loose piezoelectric layer is in contact with bone defects to play a role in guiding osteoblasts to adhere; functionally, soft tissue repair is promoted through the angiogenesis promoting effect of the acellular dermal matrix and the similarity of the tissue of the acellular dermal matrix, and bone regeneration of a bone defect area is promoted through the piezoelectric osteogenesis and angiogenesis effects of the piezoelectric layer.

The invention provides a composition for delaying cell senescence as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: loading tea polyphenol on a dog bone marrow mesenchymal stem cell exosome, and modifying the surface of the dog bone marrow mesenchymal stem cell exosome with arginine nonamer to prepare a modified tea polyphenol-loaded exosome; the preparation method comprises the following steps of: modifying cholesterol by using arginine nonamer, and preparing lipidosome by using the cholesterol modified by the arginine nonamer, DSPE-PEG2000, phospholipid embedded resveratrol and epigallotein gallate; polysaccharide is extracted from rhizoma polygonati, fructus lycii and ginseng, and rhizoma polygonati / fructus lycii / ginseng polysaccharide is prepared; the preparation method comprises the following steps: mixing an acellular dermal matrix with polygonatum sibiricum / medlar / ginseng polysaccharide, adding lipidosome and a modified tea polyphenol-loaded exosome, co-incubating, and freeze-drying to prepare the composition for delaying cell aging, the composition has a better drug embedding effect, can improve the stability and transdermal absorption effect of the drug, has better antioxidant, antibacterial and anti-inflammatory effects, and can be used for preparing the medicine for delaying cell aging. The effect of delaying skin cell senescence can be better achieved through multiple ways, and safety and degradability are achieved.

A soft tissue filling composition according to one aspect of the present invention has a high collagen content and a low lipid content, and thus has high biocompatibility and moldability without causing allergic reactions and inflammation. In addition, the composition has a high complex viscosity and storage modulus, and thus can perform an excellent supporting function, maintains homogeneous properties, and can be injected with low injection force by using a standard syringe. Accordingly, the soft tissue filling composition according to one aspect of the present invention can be used to restore the volume of defective or recessed soft tissues, for example, breasts, and can be used for alleviating skin curvature, cosmetic fillers, or supplementing moisture sensation.

The application discloses a preparation process of efficient acellular dermal matrix, and the acellular dermal matrix is prepared through the following steps: obtaining dermal tissue through pretreatment of animal fetal skin, and then performing specific acellular treatment and defatting treatment processes, and finally performing crosslinking, freeze-drying and sterilization. The acellular dermal matrix preparation process can effectively remove impurities and immunogenic substances, and at the same time, the activity of extracellular matrix is maximally protected. The obtained acellular dermal matrix product has complete triple-helix structure of collagen, low residues of DNA and alpha-Gal antigen, extremely low fat content, no cytotoxicity and high safety.

The embodiment of the application discloses a preparation method of humanized extracellular matrix, and belongs to the technical field of biomedical materials and tissue engineering. The method comprises the following steps: preparing human decellularized dermal matrix; preparing a concentrated solution of a condition medium rich in human active factors; constructing RGD-PLGA / BMP-2 nanocomposites; loading the concentrated solution of the condition medium into the human decellularized dermal matrix through vacuum negative pressure perfusion, and then anchoring the RGD-PLGA / BMP-2 nanocomposites, and cross-linking them twice through genipin to obtain the humanized extracellular matrix. Through the four-element strategy of decellularized matrix-active factor-nano-sustained release-two-stage cross-linking, the unity of low immunogenicity, high biological activity and spatiotemporal controllable release function is realized, and the humanized extracellular matrix can be applied to the fields of tissue engineering scaffolds, wound repair and bone defect filling.

The invention discloses a metabolizable and anisotropic tensile hernia patch and a preparation method thereof. The material is prepared from the following raw material components in parts by weight: 10-15 parts of a high polymer material, 80-180 parts of a solvent, 1-3 parts of a piezoelectric material, 0.05-0.15 part of an acellular dermal matrix and 8-12 parts of an acetic acid solution. Through high-speed electrostatic spinning and angle layering design, the mechanical property of the patch can be accurately regulated and controlled, and the anisotropy of an abdominal wall muscle layer is simulated. The multilayer staggered structure is similar to the effect of plywood, can effectively disperse and bear stress in multiple directions, prevents the patch from shrinking or bulging, and provides more stable and durable mechanical support.

The invention provides a porcine acellular dermal matrix for hip joint labial glenoid reconstruction as well as a preparation method and application of the porcine acellular dermal matrix, and belongs to the technical field of biomedical materials. Cleaning and incubating the natural pig dermis tissue; then treating by using a solution containing EDTA-pancreatin and a solution containing a surfactant in sequence; and finally, washing to obtain the porcine acellular dermal matrix. According to the invention, the porcine acellular dermal matrix is treated by a specific process and is used as a heterogeneous hip joint labial glenoid reconstruction material, and good biocompatibility and tissue guiding function are shown. Meanwhile, the preparation method is simple, the raw material source is wide, and the problem of graft source limitation in the existing hip joint laparolabial glenoid reconstruction operation is solved.

The invention belongs to the technical field of biological materials, and particularly relates to an acellular dermal matrix as well as a preparation method and application thereof. The preparation method comprises the following steps: placing the discarded megalovus tissue cut off from a patient in a cold chain preserving fluid, and repairing epidermis and a fat layer under a sterile condition to obtain a pretreated tissue; sequentially carrying out decellularization and depigmentation treatment on the pretreated megalovus tissues to obtain depigmented megalovus tissues; finally, stabilizing treatment and sterilization are carried out, and the acellular dermal matrix is prepared. According to the preparation method disclosed by the invention, the autologous megalovus tissue is taken as a donor, melanin and nevus cells in the tissue can be thoroughly removed, and the risks of megalovus recurrence and melanin deposition are effectively reduced; meanwhile, the natural structure and biomechanical properties of the dermal matrix can be completely reserved, waste is utilized, and reliable biomechanical support is provided for subsequent replanting application of the acellular dermal matrix.

The invention relates to immunoregulation hydrogel with antibacterial and antioxidant characteristics and a preparation method of the immunoregulation hydrogel. The immunoregulation hydrogel is prepared from nanoparticles self-assembled by procyanidine and chlorhexidine acetate, methacrylated gelatin and a porcine acellular dermal matrix. The hydrogel disclosed by the invention has good biocompatibility, self-healing capability and mechanical property, has excellent antibacterial and antioxidant properties, can effectively regulate immunity and promote wound healing, shows remarkable effects in all aspects, and has a good application prospect.

This invention belongs to the field of biomedical materials and tissue engineering technology, and provides a decellularized matrix composite hydrogel dressing, its preparation method, and its application. The decellularized matrix composite hydrogel dressing provided by this invention includes a hydrogel matrix and ginger exosome-like nanoparticles (GELNs) dispersed in the hydrogel matrix; the hydrogel matrix is ​​prepared from raw materials including decellularized dermal matrix and decellularized aortic adventitia matrix. The composite hydrogel dressing of this invention has a three-dimensional porous structure, enabling sustained release of GELNs. It also significantly accelerates the healing process of full-thickness skin defects by downregulating the TLR4 / MyD88 / NF-κB signaling pathway, synergistically regulating macrophage polarization towards the M2 type, and simultaneously promoting angiogenesis and collagen deposition. The dressing has widely available raw materials, good biocompatibility, and broad application prospects in the field of skin wound repair.

The application provides a pig acellular dermal matrix for hip joint labrum reconstruction and a preparation method and application thereof, and belongs to the technical field of biomedical materials. Natural pig dermal tissue is washed and incubated, then is treated with a solution containing EDTA-trypsin and a solution containing a surfactant in sequence, and finally is washed to obtain the pig acellular dermal matrix. The pig acellular dermal matrix is treated by a specific process, is used as a xenogenic hip joint labrum reconstruction material, and exhibits good biocompatibility and tissue guiding function. Meanwhile, the preparation method is simple, raw materials are widely sourced, and the limitation of a graft source in a current hip joint labrum reconstruction surgery is solved.

The invention relates to multifunctional in-situ repair hydrogel based on a modified dermal matrix as well as a preparation method and application of the hydrogel. The hydrogel is formed by crosslinking a first polymer precursor and a second polymer precursor through dynamic covalent bonds of boric acid ester, the first polymer precursor is carboxymethyl chitosan grafted with a phenylboronic acid group and an epsilon-polylysine chain segment; the second polymer precursor is a soluble acellular dermal matrix which is grafted with a 3, 4-dihydroxyphenyl propionic acid group and is subjected to enzymolysis treatment. The hydrogel disclosed by the invention has good biocompatibility, moldability and potential self-healing capability.

The invention relates to a universal biological acellular dermal matrix (ADM) prosthesis for use in body reconstruction processes, comprising: a first layer of ADM of biological origin, arranged at the bottom to delimit a cavity with a bottom edge; a packing material which is arranged in the cavity surrounded by the first ADM layer and consists of ground ADM material having the consistency of a fibrous pulp; and a second layer of ADM of biological origin which is arranged on the upper part and covers the packing material and the top edge of which rests, at its periphery, on the bottom edge of the first layer of ADM, thereby defining a rim; wherein the first layer of ADM and the second layer of ADM are joined by precisely arranging the upper edge of the first layer of ADM on top of the bottom edge of the rim, at the periphery, using a biocompatible glue or using sutures made of resorbable thread; wherein the first layer of ADM, the second layer of ADM and the ADM packing material are not immunogenic; and wherein the ADM comprises collagen fibres, fibronectin, elastin, laminin, glycosaminoglycans and hyaluronic acid. The invention also relates to a method for producing same.