101 results about "Macrophage polarization" patented technology

The invention relates to the technical field of medicines, in particular to a construction method and application of a photothermal conversion platform for treating osteoarthritis by regulating synovial macrophage polarization. According to the present invention, the M2 membrane-structured black phosphorus selenium carbon body (M2-BPSeC) photo-thermal nano-platform is innovatively provided, the triple functions of bionic targeting delivery, precise thermal control intervention and immune microenvironment remodeling are integrated, and the excellent synergistic treatment effect is represented in the in-vitro cell model and the OA animal model. The technology breaks through the technical bottleneck that traditional treatment is insufficient in targeting and single in intervention dimension, precise functional coupling of the physical thermal effect and immunoregulation is achieved for the first time, and a brand new strategy of targeting-temperature control-immunity integration is provided for osteoarthritis treatment. The efficient and controllable preparation process and the remarkable multi-dimensional treatment effect open up a new path for cartilage regeneration and immune balance regulation and control.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating sepsis-related acute lung injury and application thereof. The traditional Chinese medicine composition comprises the following active pharmaceutical ingredients in parts by mass: 5-15 parts of cassia twig, 5-15 parts of rheum officinale, 10-20 parts of peach kernel, 25-35 parts of gypsum and 10-20 parts of kudzu vine root. Animal experiments show that the traditional Chinese medicine composition can effectively improve lung tissue pathological manifestations of mice with sepsis-related acute lung injury by inhibiting polarization of M1 type macrophages, promoting polarization of M2 type macrophages, improving the infiltration level of neutrophils and inhibiting release of inflammatory factors. Therefore, the traditional Chinese medicine composition disclosed by the invention has a good treatment effect on acute lung injury related to sepsis.

The invention discloses an adipose-derived stem cell exosome compound loaded with silver nanoparticles and application of the adipose-derived stem cell exosome compound loaded with the silver nanoparticles, and ADSC-Exos loaded with the silver nanoparticles is developed by integrating AgNPs into an exosome of ADSCs. In vivo, the Exo-AgNPs can accelerate healing of infected wounds by reducing bacterial colonization and promoting collagen deposition, angiogenesis and M2 type macrophage polarization. In vitro, the Exo-AgNPs activates the functions of fibroblasts and vascular endothelial cells, and shows an excellent antibacterial property. In addition, the Exo-AgNPs induces polarization of M2 type macrophages and inhibits secretion of proinflammatory cytokines by activating autophagy. The results show that Exo-AgNPs is expected to become an ideal biological material for treating infected wounds.

The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in macrophage polarization and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture system is used for preparing a capture antibody coupled with a fluorescence coding microsphere by coupling a biotinylated antibody with a fluorescence microsphere coated with biotin, and the detection system is used for preparing a fluorescence labeled antibody by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody. The mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect multiple cell factors, the sample dosage is only 25 microliters, the kit is suitable for in-vitro tests of mouse mononuclear macrophage leukemia cell lines RAW264.7, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.

Disclosed in the present invention are a bionic electrothermal coupling composite material based on a pyroelectric effect, a medical kit and a use. The composite material subjected to corona poling treatment of the present invention has a surface potential of about 80 mv, the composite material can generate a pyroelectric effect by loading near-infrared light in vitro, and as can be seen from a diagram showing the variation of pyroelectric current with temperature, charge release occurs during temperature increase, thereby establishing correlation between electrical and thermal properties of a material, and realizing electrothermal effect-driven bionic coupling of the material. The synergistic effect of the piezoelectric properties and the pyroelectric effect is achieved by coupling, achieving the effects of promoting macrophage polarization and promoting tissue repair or healing. In exemplary embodiments, the charge generation capability of a material can be improved by controlling near-infrared light in vitro in a non-invasive manner, on-demand power supply based on a near-infrared light external field is realized, and the problem of insufficient long-term performance of a charged material after implanted into the body is solved.

The invention provides a compound based on aspergillus oryzae spores. The compound is composed of carbonized aspergillus oryzae spore microsphere surfaces and pores loaded with quercetin. The average porosity of the carbonized aspergillus oryzae spore microspheres is 25%-45%; the mass of the quercetin accounts for 30%-50% of the total mass of the compound. According to the compound, the solubility and bioavailability of quercetin are remarkably improved, efficient pulmonary alveolar deposition is guaranteed through the optimized aerodynamic diameter of the compound, and rapid intake of macrophages is guaranteed through the rough surface of the compound. An in-vivo and in-vitro ALI model verifies the safety of the compound QU-cASP prepared by the invention, and the compound QU-cASP has excellent effects in the aspects of targeted delivery, oxidative stress inhibition and macrophage polarization regulation. The invention also provides a method for preparing the compound, an application of the compound in preparation of a medicine for relieving acute lung injury, and an inhalable preparation taking the compound as an active ingredient.

A series of 1, 3, 5-triazine compounds that exhibit potent inhibition of endosomal trafficking and autophagy is provided. These compounds effectively suppress cancer cell proliferation, growth, and migration, induce cell cycle arrest at the G0 / G1 phase, promote cancer cell death via non-apoptotic pathways, and inhibit tumor sphere formation. Moreover, select compounds within this series demonstrate significant inhibition of tumor growth and metastasis in mouse models of lung cancer and melanoma. Additionally, they modulate macrophage polarization and the tumor microenvironment by regulating cytokine secretion. These findings highlight the potential of 1, 3, 5-triazine compounds as promising antitumor agents.

The application discloses a kind of nanoparticles for tumor sonodynamic combined individualized immunotherapy and its preparation method and application, belong to the field of biological medicine.The nanoparticles for tumor sonodynamic combined individualized immunotherapy are double-shell core structure nanoparticles formed by inner core and shell, and the inner core is formed by self-assembly of TLR3 agonist Poly (I:C), L-arginine and polylysine, and the shell is hyaluronic acid layer HA-Ce6 conjugated with photosensitizer Ce6.It is found through experiment verification that the nanoparticles can target tumor, generate reactive oxygen species to kill tumor cells under the action of ultrasound, promote M1 macrophage polarization and produce NO release at the same time, play the role of antitumor and immune effect, and realize the combined application of sonodynamic and NO gas therapy.The nanoparticles for tumor sonodynamic combined individualized immunotherapy can trigger systemic immune response, inhibit primary tumor, produce immune memory effect, and prevent tumor metastasis and recurrence.

The invention discloses an indole pyridine chalcone compound and application thereof in medicine for treating colitis, and belongs to the technical field of biological medicine. The compound has a structure as shown in a formula I, wherein R1 is selected from C1-C8 alkyl, C1-C8 alkoxy, halogen, cyano, nitryl and morpholinyl, and R2 is selected from nitryl. According to the invention, multiple excellent pharmacophores such as indole, pyridine, chalcone, sulfonamide and the like are fused in the same molecule for the first time, the preparation method does not need pre-activation of a substrate, does not need a metal catalyst or an additive, and has the advantages of mild reaction conditions, high yield, good selectivity, low cost and environmental friendliness. The compound has strong inhibitory activity on hexokinase 2 (HK2), can significantly inhibit inflammatory response and regulate macrophage polarization, shows an excellent colitis treatment effect in in-vivo and in-vitro experiments, provides a new choice for developing novel colitis treatment drugs, and has a wide medical application prospect.

The application discloses a degradable polymer-based bone repair 3D printing composite material and a preparation method and application thereof, and belongs to the technical field of bone repair material preparation.The raw material of the degradable polymer-based bone repair 3D printing composite material comprises a degradable polymer, a compatibilizer and hydroxyapatite and Mg(OH)2 co-loaded chitosan microspheres.The degradable polymer-based bone repair 3D printing composite material has good mechanical properties, biocompatibility, mineralization capacity, antibacterial and anti-inflammatory properties, can be used as a bone repair material, and is used for preparing a bone repair product through 3D printing after being prepared into a bone repair 3D printing wire material through extrusion molding, thereby providing a novel material solution for bone tissue regeneration and repair.The chitosan can adjust macrophage polarization and reduce the expression of inflammatory factors, so that the composite material has anti-inflammatory properties.

The invention discloses a bionic bimetallic polyphenol network nano-drug loaded with small non-coding RNA and a preparation method and application thereof, and belongs to the technical field of nano biomedicine.The nano-drug is loaded with small interference through a bimetallic polyphenol network, the surface of the nano-drug is coated with an M1 type macrophage membrane, and the safe and effective nano-drug is formed; the nano-drug acts on macrophages of an inflammation joint part, ROS is synergistically cleared by releasing tannic acid, Cu < 2 + > and small interfering RNA, TNF-alpha expression is inhibited, macrophage polarization is induced, meanwhile, Sr < 2 + > further plays a role in cartilage protection, and the purpose of treating osteoporosis and rheumatoid arthritis is achieved.

The invention relates to the technical field of biomedical materials. The invention discloses a Schiff base-sulfonated hyaluronic acid light-cured microsphere as well as a preparation method and application thereof. The microspheres are prepared by condensing DL-lysine and paeonol to generate Schiff base, then conjugating the Schiff base with sulfonated hyaluronic acid through a carbodiimide chemical method, and finally molding by adopting a microfluidic technology and carrying out ultraviolet curing. The metal chelation characteristic of Schiff base is combined with the precise structure regulation advantage of the microspheres, and the prepared microspheres show an excellent synergistic effect when applied to the surfaces of implant materials such as degradable magnesium alloy, iron alloy and zinc alloy: the corrosion resistance of a base material can be remarkably improved, an effective protective layer is formed, and the service life of the microspheres is prolonged. Meanwhile, the compound has multiple biological functions of promoting endothelial cell proliferation, inhibiting excessive proliferation of smooth muscle cells and regulating and controlling macrophages to be polarized to anti-inflammatory phenotypes, and has a wide application prospect in the biomedical fields of intravascular stents, bone fixing instruments and the like.

The invention discloses an implant with a honeycomb-shaped micro-nano topological structure on the surface and a preparation method and application thereof, and the implant with the honeycomb-shaped micro-nano topological structure on the surface is a honeycomb-shaped micro-nano topological structure layer prepared on the surface of a titanium substrate through a template method. According to the invention, a unique hydrogenated titanium dioxide honeycomb-shaped surface micro-nano topological structure is designed on the surface of the titanium-based implant through a template method and plasma hydrotreating on the basis of not introducing an antibacterial substance coating and complex chemical components; the special surface treatment not only has excellent 800-815nm near-infrared light response photocatalytic performance and can generate a large number of reactive oxygen species to quickly eliminate bacterial infection, but also can regulate polarization of macrophages due to the unique surface morphology, effectively inhibit inflammatory response and regulate and control bone immune microenvironment to promote osteogenesis, and has a good application prospect. And therefore, more than two invasive operations are not required to be carried out on the patient, and the postoperative healing effect is better.

This invention belongs to the field of biomedical materials technology, specifically disclosing a core-shell microneedle, a core-shell microneedle patch, its preparation method, and its application. Through structural design and carrier material optimization, this invention provides a core-shell microneedle with a programmed sequential release of tannic acid in the outer shell and verteporfen in the core layer, successfully matching the dynamic physiological rhythm of wound healing and ensuring effective wound repair. In vitro and in vivo biological evaluations have confirmed that the core-shell microneedle patch prepared using the core-shell microneedles of this invention possesses excellent antibacterial, antioxidant, and immune microenvironment-regulating abilities. It can effectively induce macrophage polarization towards the M2 type, specifically inhibit YAP protein expression, and significantly accelerate the closure process of infected wounds. From both upstream immune regulation and downstream fibrosis inhibition levels, it dually blocks the pathological scar formation pathway, ultimately achieving high-quality, scar-free regenerative repair of infected wounds.

This invention provides a pH-responsive immunomodulatory microneedle patch, its preparation method, and its application, belonging to the field of biomedical materials technology. This invention incorporates Zif-8 nanoparticles loaded with ChABC into GelMA hydrogel to prepare a pH-responsive immunomodulatory microneedle patch (Z@C-MN). As the patch gradually degrades, the system can continuously release Zn in a pH-responsive manner. 2+ Z@C-MN, along with ChABC, promotes in situ repair of cerebral fibrosis (SCI) after injury. Z@C-MN induces macrophage polarization towards a pro-regenerative phenotype, thereby downregulating the inflammatory response, increasing neurite length, and promoting the differentiation of neural stem cells into neurons. In vivo, Z@C-MN reduced the inflammatory microenvironment after injury and promoted angiogenesis in a rat SCI model. Furthermore, it activated axonal regeneration by inhibiting scar tissue formation and regulating CD4+. + T cell transformation supports neurogenesis.

The invention provides a preparation method of dynamic connection bone extracellular matrix simulation hydrogel and application of the dynamic connection bone extracellular matrix simulation hydrogel in bone defect repair, and belongs to the technical field of medical hydrogel. L-cysteine ethyl ester functionalized hyaluronic acid and allicin are prepared to form a thiol-disulfide bond dynamic cross-linked network; a sacrificial pre-vascularization template is constructed by combining a collagen thread loaded with a vascular endothelial growth factor, a vascularization guide microchannel is formed by utilizing the enzymolysis characteristic of collagen, the polarization of macrophages is regulated and controlled by hyaluronic acid to inhibit inflammatory response, and the adhesion and osteogenic differentiation of mesenchymal stem cells are promoted by virtue of a functional surface, so that the osteogenic differentiation of the mesenchymal stem cells is promoted. The synergistic effect of vascularization, anti-inflammation and osseointegration is achieved, finally, the dynamic connection bone extracellular matrix simulation hydrogel with a porous interconnection structure and biological activity is formed, and the technical problems that a traditional bone defect repair material is insufficient in vascularization, inflammation interference exists, and osseointegration is poor are solved.

The invention discloses a preparation method of luteolin-loaded targeted polypeptide nanoparticles and application of the luteolin-loaded targeted polypeptide nanoparticles in hepatic fibrosis treatment, and belongs to the technical field of nano-drug delivery and liver disease treatment. The luteolin-loaded targeted polypeptide nanoparticles are used for treating or relieving liver fibrosis related diseases. According to the nanoparticle disclosed by the invention, a stable structure is formed through self-assembly of the amphiphilic polymer, the luteolin is physically entrapped, targeted delivery of liver immune cells is realized, and the nanoparticle shows good biocompatibility and safety in an in-vivo macrophage polarization and liver fibrosis model; the inflammatory response and collagenous fiber deposition can be remarkably inhibited, and the hepatic tissue fibrosis degree is improved. Compared with the existing anti-hepatic fibrosis medicine which has the problems of poor targeting property, low bioavailability, obvious side effect after long-term medication and the like, the luteolin-loaded targeting polypeptide nanoparticle has the remarkable advantages of high delivery efficiency, strong targeting property, good safety, stable treatment effect and the like.

This invention relates to the field of medical materials technology, providing a hydrogel containing antler mesenchymal stem cell exosomes, its preparation method, and applications. The method includes the following steps: preparation of antler mesenchymal stem cell exosomes; preparation of PVP-HA composite hydrogel; uniformly mixing AMSC-Exos with a hydrogel precursor solution, and forming a hydrogel containing antler mesenchymal stem cell exosomes through physical embedding and non-covalent interactions. This invention utilizes the three-dimensional porous network structure of the hydrogel to achieve long-term sustained release of exosomes, protecting them from rapid degradation; it leverages the unique regenerative advantages of antler-derived exosomes to synergistically promote angiogenesis, inhibit inflammatory responses, and regulate macrophage polarization; it promotes angiogenesis through the miR-486-5p / FGF9 axis, achieving efficient repair of chronic diabetic wounds; and it provides a novel, efficient, safe, and clinically translational strategy for the treatment of chronic diabetic skin lesions.

Disclosed herein is a compound of formula (I): (I) where: A represents a tandem-lock biomarker responsive group; B represents a self-immolative linker; and C represents a chemiluminescent group, or a pharmaceutically acceptable salt or solvate thereof.

This invention discloses a composite scaffold for promoting bone regeneration in diabetic patients, its preparation method, and its applications, relating to the field of medical biomaterials technology. The composite scaffold for promoting bone regeneration in diabetic patients comprises: a three-dimensional porous framework; and a bioactive hydrogel layer loaded with naringin. The bioactive hydrogel layer loaded with naringin is coated on the surface and pore walls of the three-dimensional porous framework. The technical solution provided by this invention uses a 3D-printed PLGA / HA porous framework to provide mechanical support and osteoconductivity, and a supramolecular hydrogel coating loaded with naringin to endow it with immunomodulatory and drug sustained-release functions. This scaffold has good hydrophilicity and biocompatibility, and can effectively induce macrophage polarization towards the M2 anti-inflammatory phenotype by activating the JAK-STAT signaling pathway, improving the local inflammatory microenvironment of diabetic bone defects. Simultaneously, it indirectly promotes vascular endothelial cell migration, lumen formation, and osteogenic differentiation of bone marrow mesenchymal stem cells by regulating macrophages.

The application discloses application of a small-molecule compound LZ-A4 in preparation of a preparation or a medicine for treating malignant tumors. 19 H 11 Cl2N3O3S, which can promote LGMN degradation in malignant tumor cells, inhibit tumor cell proliferation, and inhibit macrophage polarization to M2 type. The application proves through research on a colorectal cancer mouse transplanted tumor model that LZ-A4 alone can effectively inhibit the progression of colorectal cancer, and after being combined with a colorectal cancer first-line chemotherapy medicine oxaliplatin, the tumor volume can be further reduced. Meanwhile, LZ-A4 can inhibit M2 type polarization of macrophages in a tumor microenvironment in vivo, promote infiltration of cytotoxic T cells, reshape the tumor microenvironment, and activate anti-tumor immunity. In addition, A4 performs well in terms of toxic side effects, and after being combined with the chemotherapy medicine, the mouse weight is not further reduced.

The invention discloses a bimodal nucleic acid removal system for treating an intervertebral disc degenerative disease as well as a preparation method and an application method of the bimodal nucleic acid removal system. A nucleus pulposus cell membrane vesicle is used for entrapping a small molecule mitochondrial membrane stabilizer, and the engineered nano vesicle is prepared. The preparation method comprises the following steps: mixing a polyamidoamine (PAMAM) dendritic polymer with a hyaluronic acid solution, adding the PAMAM dendritic polymer, and carrying out Schiff alkali reaction crosslinking to form hydrogel, so as to obtain the bimodal nucleic acid removal system. The hydrogel is combined with negative cfDNA released in tissue gaps through positive charges, inflammatory nucleic acid in the tissue microenvironment is efficiently removed, polarization of M1 type macrophages and release of inflammatory factors are inhibited, and the local immune microenvironment is improved. The engineered nanovesicles are used for targeted delivery of a small-molecule mitochondrial membrane stabilizer into nucleus pulposus cells, DNA leakage caused by increase of permeability of mitochondrial outer membranes is inhibited, the cell steady state is recovered, cell function degeneration is delayed, and inflammatory response is inhibited from two aspects of intracellular sources and tissue external environments through synergistic effects.

A method for treating a disease or disorder includes administering to a subject an effective amount of an agent that promotes the polarization of macrophages. The macrophages selectively repolarize their phenotype in the microenvironment. The agent can be PKM2 or mutant thereof. Further, wherein administration of the agent reduces inflammation in the subject.

The application discloses the technical field of carbon dot application, and relates to a luteolin carbon dot for guiding lysosomes and a preparation method and application thereof. The luteolin carbon dot is prepared by using luteolin and 2,5-dihydroxyterephthalic acid as carbon source precursors through a hydrothermal method. The prepared luteolin carbon dot has a particle size of 0.5-4 nm, a fluorescence excitation wavelength of 360-440 nm, a maximum emission wavelength of 540 nm and a Zeta potential of -19 mv. Research shows that the luteolin carbon dot can guide macrophage and adipocyte lysosomes, promote M2 type macrophage polarization, and up-regulate the expression of the heat production and brown colorization marker gene Ucp1 of beige adipocytes. Further, the M2 type macrophage loaded with the luteolin carbon dot can promote the expression of the Ucp1 of the beige adipocyte through the release of the carbon dot and the paracrine effect, thereby increasing heat production and energy consumption. The application lays a foundation for developing an intervention and treatment scheme for obesity and related metabolic diseases based on the carbon dot in the future.

The present application provides a kind of complex based on aspergillus oryzae spore, it is constituted by carbonized aspergillus oryzae spore microsphere surface and the quercetin loaded in aperture;The average porosity of the carbonized aspergillus oryzae spore microsphere is 25%~45%;The mass of the quercetin accounts for 30%‑50% of the total mass of the complex.The complex of the present application significantly improves the solubility and bioavailability of quercetin, and the optimized aerodynamic diameter ensures efficient alveolar deposition, and the rough surface ensures the rapid uptake of macrophages.In vivo and in vitro ALI model verifies the safety of the complex QU-cASP prepared by the present application and has excellent effect in targeted delivery, inhibition of oxidative stress and regulation of macrophage polarization.The present application also provides a method for preparing the complex, the application of the complex in preparing a drug for relieving acute lung injury, and an inhalable preparation with the complex as the active ingredient.

The invention discloses a polysaccharide-polyphenol-metal compound for preventing colorectal cancer related to colitis, the polysaccharide-polyphenol-metal compound is formed by compounding a metal-polyphenol nano ligand and polysaccharide molecules, and the metal-polyphenol nano ligand is a nano complex formed by tea polyphenol and metal ions through coordination. The invention also discloses a preparation method and application thereof. The compound has the functions of oxidation resistance, inflammation resistance, immune metabolism reprogramming, intestinal flora steady-state regulation and control and the like, and can activate an Nrf2-ARE antioxidant pathway and restore expression of epithelial tight junction protein by adjusting polarization of macrophages and inhibiting NF-kB / STAT3 proinflammatory signals, thereby promoting reconstruction of beneficial flora (such as Lactobacillus and Akkermansia). The composite system can also achieve the synergistic effect of immunoregulation and chemotherapy combined cancer prevention under the drug loading condition, and the canceration process of colitis is remarkably delayed.

The invention belongs to the technical field of medicines, and particularly relates to a microneedle patch for disguising a macrophage cell membrane and a preparation method of the microneedle patch. Degradable high polymer material nano-particles camouflaged by the macrophage membrane are embedded in a microneedle array of the microneedle patch camouflaged by the macrophage membrane, and the degradable high polymer material nano-particles camouflaged by the macrophage membrane are wrapped with gambogic acid and oleic acid functionalized cerium dioxide nano-particles. Nanoparticles of the microneedle patch jointly load gambogic acid and oleic acid functionalized cerium dioxide, through microneedle transdermal drug delivery, active targeting of the drug to the liver is achieved by using natural inflammatory taxis of a macrophage membrane, the anti-inflammatory effect of gambogic acid and the anti-oxidation effect of cerium dioxide are synergistically exerted, polarization of macrophages is adjusted, and the anti-inflammatory effect of the drug on the liver is achieved. Therefore, the liver injury caused by the septic shock is treated.

The invention belongs to the technical field of medicines and disease treatment, and particularly relates to application of SEC24D in preparation of medicines for treating atherosclerosis. The amino acid sequence of the SEC24D is as shown in SEQ ID NO.1. The medicine is a preparation which takes the SEC24D as a target spot and can inhibit the expression of the SEC24D. Clinical data and basic experiments prove that the SEC24D plays an important role in inflammation, the mRNA level of whole blood cells of the SEC24D is increased to reflect the activation of the inflammation, and we find that the mRNA level and protein level of the SEC24D are increased in the polarization process of M1 macrophages, the expression of the macrophages SEC24D is reduced, the functions of the M1 macrophages can be inhibited, and inflammatory mediators can be reduced.

The invention discloses a wound repair system based on metformin hydrogel and ultrasound cooperation and application of the wound repair system in promoting wound repair. The system comprises an ultrasonic stimulation unit, a gel release unit, a core control unit and a wound monitoring unit. The ultrasonic stimulation unit acts on the wound at the frequency of 20 kHz to 3 MHz and the strength of 0.1 W / cm to 3 W / cm; and the gel release unit is used for conveying hydrogel containing 0.5 to 3 weight percent of metformin and 1 to 10 weight percent of oxidized dextran. The core control unit regulates and controls ultrasonic parameters and gel release according to feedback of the monitoring unit, and the ultrasonic parameters and the gel release can work synchronously or successively. According to the system, macrophage polarization and hydrogel continuous drug release are regulated and controlled through ultrasound, inflammation is improved, revascularization is promoted, and an effective treatment strategy is provided for chronic wound surfaces difficult to heal.

The invention relates to a multifunctional unilateral adhesion hydrogel and application thereof in tendon injury repair. The multifunctional single-side adhesive hydrogel comprises an adhesive side and a non-adhesive side, the adhesive side is P / A / TA, and the non-adhesive side is P / A / TA-Fe. The implant has high adhesion performance, high mechanical strength, an integrated structure and good biocompatibility, can fully adapt to the physiological environment and stress condition of tendons, and ensures the stability and completeness of in-vivo implantation; in addition, the components are simple and easy to prepare, the hydrogel has multiple biological functions of resisting bacteria, resisting adhesion, resisting oxidation, resisting inflammation and the like at the same time, and different degrees of macrophage polarization regulation and control can be achieved on the adhesion side and the non-adhesion side of the hydrogel through functional heterogeneity formed by tannic acid (TA) and TA-Fe differential distribution; in addition, tendon shape remodeling and tissue regeneration are remarkably promoted in vivo, and the method can be used for tendon injury repair.