155 results about "Macrophage polarization" patented technology

The invention discloses a hydrogel dressing with a double-slow-release effect as well as a preparation method and application of the hydrogel dressing. The preparation method of the hydrogel dressing comprises the following steps: firstly, preparing methyl acrylate modified hyaluronic acid capable of being photo-crosslinked, then preparing carboxymethyl chitosan nanoparticles loaded with chlorogenic acid, and finally, mixing the methyl acrylate modified hyaluronic acid and the carboxymethyl chitosan nanoparticles loaded with chlorogenic acid to obtain the hydrogel dressing. The hydrogel dressing embedded with the nanoparticles is obtained in a photo-crosslinking mode. The hydrogel dressing provided by the invention not only has good biocompatibility and mechanical properties, but also plays an anti-oxidation role by removing free radicals in a body, and can effectively kill bacteria such as escherichia coli and staphylococcus aureus to play an antibacterial role. The hydrogel dressing can also reduce excessive inflammatory response in the wound healing process by influencing macrophage polarization, promote chronic wound healing and relieve pain of patients.

The invention relates to the technical field of medicines, in particular to a construction method and application of a photothermal conversion platform for treating osteoarthritis by regulating synovial macrophage polarization. According to the present invention, the M2 membrane-structured black phosphorus selenium carbon body (M2-BPSeC) photo-thermal nano-platform is innovatively provided, the triple functions of bionic targeting delivery, precise thermal control intervention and immune microenvironment remodeling are integrated, and the excellent synergistic treatment effect is represented in the in-vitro cell model and the OA animal model. The technology breaks through the technical bottleneck that traditional treatment is insufficient in targeting and single in intervention dimension, precise functional coupling of the physical thermal effect and immunoregulation is achieved for the first time, and a brand new strategy of targeting-temperature control-immunity integration is provided for osteoarthritis treatment. The efficient and controllable preparation process and the remarkable multi-dimensional treatment effect open up a new path for cartilage regeneration and immune balance regulation and control.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating sepsis-related acute lung injury and application thereof. The traditional Chinese medicine composition comprises the following active pharmaceutical ingredients in parts by mass: 5-15 parts of cassia twig, 5-15 parts of rheum officinale, 10-20 parts of peach kernel, 25-35 parts of gypsum and 10-20 parts of kudzu vine root. Animal experiments show that the traditional Chinese medicine composition can effectively improve lung tissue pathological manifestations of mice with sepsis-related acute lung injury by inhibiting polarization of M1 type macrophages, promoting polarization of M2 type macrophages, improving the infiltration level of neutrophils and inhibiting release of inflammatory factors. Therefore, the traditional Chinese medicine composition disclosed by the invention has a good treatment effect on acute lung injury related to sepsis.

The invention discloses an engineered macrophage and a pharmaceutical composition and application thereof, and CCM3 expression in the engineered macrophage is inhibited, so that the engineered macrophage is polarized towards the M2 direction or the engineered macrophage is an M2 type macrophage. Besides, the invention further develops a temperature-sensitive cell-loaded hydrogel preparation which can promote polarization of macrophages in the M2 direction by inhibiting expression of signal molecules CCM3 on adhesion spots, and the temperature-sensitive cell-loaded hydrogel preparation can promote bone defect immune osteogenesis through local injection.

The invention discloses an adipose-derived stem cell exosome compound loaded with silver nanoparticles and application of the adipose-derived stem cell exosome compound loaded with the silver nanoparticles, and ADSC-Exos loaded with the silver nanoparticles is developed by integrating AgNPs into an exosome of ADSCs. In vivo, the Exo-AgNPs can accelerate healing of infected wounds by reducing bacterial colonization and promoting collagen deposition, angiogenesis and M2 type macrophage polarization. In vitro, the Exo-AgNPs activates the functions of fibroblasts and vascular endothelial cells, and shows an excellent antibacterial property. In addition, the Exo-AgNPs induces polarization of M2 type macrophages and inhibits secretion of proinflammatory cytokines by activating autophagy. The results show that Exo-AgNPs is expected to become an ideal biological material for treating infected wounds.

The invention provides application of adiponectin and an adiponectin receptor agonist in treatment of amyotrophic lateral sclerosis. The invention finds that the plasma adiponectin level of an ALS patient is related to the inflammatory factor level, and finds that AdipoR1 and AdipoR2 in the ALS patient are remarkably up-regulated and are related to M1 type macrophage activation. Experiments prove that the adiponectin receptor stimulant can relieve the proinflammatory response of macrophages, regulate the polarization of the macrophages and protect neurons, and the adiponectin receptor stimulant can postpone the occurrence of dyskinesia of ALS patients and prolong the lifetime of the ALS patients. The invention provides a new strategy for diagnosis and treatment of ALS, and has a good clinical application prospect.

The invention provides a piezoelectric dressing for promoting nerve repair of a diabetic wound and a preparation method and application of the piezoelectric dressing, and belongs to the technical field of piezoelectric dressings. The preparation method comprises the following steps: firstly, respectively preparing a polymer solution and a conductive polymer dispersion liquid, then mixing the polymer solution and the conductive polymer dispersion liquid, carrying out secondary stirring to obtain an electrostatic spinning solution, and then carrying out electrostatic spinning and post-treatment in sequence to obtain the piezoelectric dressing for promoting neural restoration of the diabetic wound. By optimizing the ratio of the raw materials and cooperating with an electrostatic spinning process, triple functions of high-strength piezoelectric response, peripheral nerve repair and macrophage polarization regulation are realized, and the separation limitation of the prior art on nerve repair and immune microenvironment dynamic regulation is broken through; and an integrated solution of electrical stimulation, nerve regeneration, immune regulation and wound healing is provided for the diabetic wound.

The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in macrophage polarization and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture system is used for preparing a capture antibody coupled with a fluorescence coding microsphere by coupling a biotinylated antibody with a fluorescence microsphere coated with biotin, and the detection system is used for preparing a fluorescence labeled antibody by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody. The mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect multiple cell factors, the sample dosage is only 25 microliters, the kit is suitable for in-vitro tests of mouse mononuclear macrophage leukemia cell lines RAW264.7, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.

According to the magnetic multifunctional nano-drug, on the basis of traditional ZIF-8, iron ions and defect structures are introduced, a synergistic antibacterial mode of magnetocaloric therapy-sonodynamic therapy-chemodynamic therapy is established for the first time, heat is generated under an alternating magnetic field, and the magnetic multifunctional nano-drug is used for treating bacterial pneumonia. Active oxygen is rapidly generated under ultrasound, existence of iron ions causes Fenton reaction to achieve chemical power therapy and induce ferroptosis to achieve synergistic antibiosis, release of zinc ions promotes polarization of macrophages to reduce inflammation of disease sites, the problems that focus depth is deep, drug resistance is generated, single treatment effect is poor and the like can be solved, and the treatment effect is good. A new thought is provided for treating the bacterial pneumonia.

Disclosed in the present invention are a bionic electrothermal coupling composite material based on a pyroelectric effect, a medical kit and a use. The composite material subjected to corona poling treatment of the present invention has a surface potential of about 80 mv, the composite material can generate a pyroelectric effect by loading near-infrared light in vitro, and as can be seen from a diagram showing the variation of pyroelectric current with temperature, charge release occurs during temperature increase, thereby establishing correlation between electrical and thermal properties of a material, and realizing electrothermal effect-driven bionic coupling of the material. The synergistic effect of the piezoelectric properties and the pyroelectric effect is achieved by coupling, achieving the effects of promoting macrophage polarization and promoting tissue repair or healing. In exemplary embodiments, the charge generation capability of a material can be improved by controlling near-infrared light in vitro in a non-invasive manner, on-demand power supply based on a near-infrared light external field is realized, and the problem of insufficient long-term performance of a charged material after implanted into the body is solved.

The invention provides a compound based on aspergillus oryzae spores. The compound is composed of carbonized aspergillus oryzae spore microsphere surfaces and pores loaded with quercetin. The average porosity of the carbonized aspergillus oryzae spore microspheres is 25%-45%; the mass of the quercetin accounts for 30%-50% of the total mass of the compound. According to the compound, the solubility and bioavailability of quercetin are remarkably improved, efficient pulmonary alveolar deposition is guaranteed through the optimized aerodynamic diameter of the compound, and rapid intake of macrophages is guaranteed through the rough surface of the compound. An in-vivo and in-vitro ALI model verifies the safety of the compound QU-cASP prepared by the invention, and the compound QU-cASP has excellent effects in the aspects of targeted delivery, oxidative stress inhibition and macrophage polarization regulation. The invention also provides a method for preparing the compound, an application of the compound in preparation of a medicine for relieving acute lung injury, and an inhalable preparation taking the compound as an active ingredient.

A series of 1, 3, 5-triazine compounds that exhibit potent inhibition of endosomal trafficking and autophagy is provided. These compounds effectively suppress cancer cell proliferation, growth, and migration, induce cell cycle arrest at the G0 / G1 phase, promote cancer cell death via non-apoptotic pathways, and inhibit tumor sphere formation. Moreover, select compounds within this series demonstrate significant inhibition of tumor growth and metastasis in mouse models of lung cancer and melanoma. Additionally, they modulate macrophage polarization and the tumor microenvironment by regulating cytokine secretion. These findings highlight the potential of 1, 3, 5-triazine compounds as promising antitumor agents.

The invention discloses an application of didymoside in a medicine for promoting postoperative tendon-bone interface fibrous cartilage regeneration. According to the application, an important way for treating the tendon-bone interface injury and preventing the tendon-bone healing disorder is obtained through the research of macrophages on fibrous cartilage tendon-bone interface repair and the influence of didymoside on interface bone formation and structure, fibrous cartilage formation and macrophage polarization regulation, so that the tendon-bone interface injury is further treated.

The invention belongs to the technical field of biological medicine, and particularly provides application of a PLA1A gene / protein as an anti-tumor target. The key effect of the PLA1A gene / protein in promoting chemotherapy drug resistance is disclosed for the first time, and a new mechanism that the PLA1A gene / protein affects chemotherapy sensitivity by regulating a tumor microenvironment (especially macrophage polarization) is deeply clarified. Researches prove that knockdown of the PLA1A gene or inhibition of the protein activity of the PLA1A gene can significantly enhance the sensitivity of tumor cells to chemotherapeutic drugs (such as oxaliplatin), so that the therapeutic effect is improved, and the sensitization provides a new strategy for overcoming tumor chemotherapeutic drug resistance. In animal model experiments, knockdown of the PLA1A is combined with oxaliplatin for treatment, so that growth of mouse transplanted tumors and liver metastases is remarkably inhibited, the survival time of tumor-bearing mice is prolonged, and it is indicated that the PLA1A has important clinical application value and wide application prospects as an anti-tumor target.

The application discloses a kind of nanoparticles for tumor sonodynamic combined individualized immunotherapy and its preparation method and application, belong to the field of biological medicine.The nanoparticles for tumor sonodynamic combined individualized immunotherapy are double-shell core structure nanoparticles formed by inner core and shell, and the inner core is formed by self-assembly of TLR3 agonist Poly (I:C), L-arginine and polylysine, and the shell is hyaluronic acid layer HA-Ce6 conjugated with photosensitizer Ce6.It is found through experiment verification that the nanoparticles can target tumor, generate reactive oxygen species to kill tumor cells under the action of ultrasound, promote M1 macrophage polarization and produce NO release at the same time, play the role of antitumor and immune effect, and realize the combined application of sonodynamic and NO gas therapy.The nanoparticles for tumor sonodynamic combined individualized immunotherapy can trigger systemic immune response, inhibit primary tumor, produce immune memory effect, and prevent tumor metastasis and recurrence.

The invention discloses an indole pyridine chalcone compound and application thereof in medicine for treating colitis, and belongs to the technical field of biological medicine. The compound has a structure as shown in a formula I, wherein R1 is selected from C1-C8 alkyl, C1-C8 alkoxy, halogen, cyano, nitryl and morpholinyl, and R2 is selected from nitryl. According to the invention, multiple excellent pharmacophores such as indole, pyridine, chalcone, sulfonamide and the like are fused in the same molecule for the first time, the preparation method does not need pre-activation of a substrate, does not need a metal catalyst or an additive, and has the advantages of mild reaction conditions, high yield, good selectivity, low cost and environmental friendliness. The compound has strong inhibitory activity on hexokinase 2 (HK2), can significantly inhibit inflammatory response and regulate macrophage polarization, shows an excellent colitis treatment effect in in-vivo and in-vitro experiments, provides a new choice for developing novel colitis treatment drugs, and has a wide medical application prospect.

The application discloses a degradable polymer-based bone repair 3D printing composite material and a preparation method and application thereof, and belongs to the technical field of bone repair material preparation.The raw material of the degradable polymer-based bone repair 3D printing composite material comprises a degradable polymer, a compatibilizer and hydroxyapatite and Mg(OH)2 co-loaded chitosan microspheres.The degradable polymer-based bone repair 3D printing composite material has good mechanical properties, biocompatibility, mineralization capacity, antibacterial and anti-inflammatory properties, can be used as a bone repair material, and is used for preparing a bone repair product through 3D printing after being prepared into a bone repair 3D printing wire material through extrusion molding, thereby providing a novel material solution for bone tissue regeneration and repair.The chitosan can adjust macrophage polarization and reduce the expression of inflammatory factors, so that the composite material has anti-inflammatory properties.

The invention discloses a bionic bimetallic polyphenol network nano-drug loaded with small non-coding RNA and a preparation method and application thereof, and belongs to the technical field of nano biomedicine.The nano-drug is loaded with small interference through a bimetallic polyphenol network, the surface of the nano-drug is coated with an M1 type macrophage membrane, and the safe and effective nano-drug is formed; the nano-drug acts on macrophages of an inflammation joint part, ROS is synergistically cleared by releasing tannic acid, Cu < 2 + > and small interfering RNA, TNF-alpha expression is inhibited, macrophage polarization is induced, meanwhile, Sr < 2 + > further plays a role in cartilage protection, and the purpose of treating osteoporosis and rheumatoid arthritis is achieved.

The invention relates to the technical field of biomedical materials. The invention discloses a Schiff base-sulfonated hyaluronic acid light-cured microsphere as well as a preparation method and application thereof. The microspheres are prepared by condensing DL-lysine and paeonol to generate Schiff base, then conjugating the Schiff base with sulfonated hyaluronic acid through a carbodiimide chemical method, and finally molding by adopting a microfluidic technology and carrying out ultraviolet curing. The metal chelation characteristic of Schiff base is combined with the precise structure regulation advantage of the microspheres, and the prepared microspheres show an excellent synergistic effect when applied to the surfaces of implant materials such as degradable magnesium alloy, iron alloy and zinc alloy: the corrosion resistance of a base material can be remarkably improved, an effective protective layer is formed, and the service life of the microspheres is prolonged. Meanwhile, the compound has multiple biological functions of promoting endothelial cell proliferation, inhibiting excessive proliferation of smooth muscle cells and regulating and controlling macrophages to be polarized to anti-inflammatory phenotypes, and has a wide application prospect in the biomedical fields of intravascular stents, bone fixing instruments and the like.

The invention discloses an implant with a honeycomb-shaped micro-nano topological structure on the surface and a preparation method and application thereof, and the implant with the honeycomb-shaped micro-nano topological structure on the surface is a honeycomb-shaped micro-nano topological structure layer prepared on the surface of a titanium substrate through a template method. According to the invention, a unique hydrogenated titanium dioxide honeycomb-shaped surface micro-nano topological structure is designed on the surface of the titanium-based implant through a template method and plasma hydrotreating on the basis of not introducing an antibacterial substance coating and complex chemical components; the special surface treatment not only has excellent 800-815nm near-infrared light response photocatalytic performance and can generate a large number of reactive oxygen species to quickly eliminate bacterial infection, but also can regulate polarization of macrophages due to the unique surface morphology, effectively inhibit inflammatory response and regulate and control bone immune microenvironment to promote osteogenesis, and has a good application prospect. And therefore, more than two invasive operations are not required to be carried out on the patient, and the postoperative healing effect is better.

This invention belongs to the field of biomedical materials technology, specifically disclosing a core-shell microneedle, a core-shell microneedle patch, its preparation method, and its application. Through structural design and carrier material optimization, this invention provides a core-shell microneedle with a programmed sequential release of tannic acid in the outer shell and verteporfen in the core layer, successfully matching the dynamic physiological rhythm of wound healing and ensuring effective wound repair. In vitro and in vivo biological evaluations have confirmed that the core-shell microneedle patch prepared using the core-shell microneedles of this invention possesses excellent antibacterial, antioxidant, and immune microenvironment-regulating abilities. It can effectively induce macrophage polarization towards the M2 type, specifically inhibit YAP protein expression, and significantly accelerate the closure process of infected wounds. From both upstream immune regulation and downstream fibrosis inhibition levels, it dually blocks the pathological scar formation pathway, ultimately achieving high-quality, scar-free regenerative repair of infected wounds.

The invention discloses application of Wilms oncoprotein-1 (WTAP) related protein in rheumatoid arthritis, finds that the specific expression level of WTAP in RA is increased for the first time, and finds that the WTAP regulates the polarization reaction of macrophages in a targeted manner so as to regulate the inflammation level of RA through overexpression and interference of WTAP and detection and verification of a WB method and an RT-qPCR method. Therefore, the preparation for detecting Wilms oncoprotein-1 (WTAP) related protein can be used for preparing a rheumatoid arthritis diagnosis preparation. The invention verifies the correlation between WTAP and RA inflammatory response and the regulation function on inflammation, and the WTAP has higher detection sensitivity and specificity on RA disease activity, and can be used for evaluating RA disease progression and relieving RA inflammatory response.

This invention provides a pH-responsive immunomodulatory microneedle patch, its preparation method, and its application, belonging to the field of biomedical materials technology. This invention incorporates Zif-8 nanoparticles loaded with ChABC into GelMA hydrogel to prepare a pH-responsive immunomodulatory microneedle patch (Z@C-MN). As the patch gradually degrades, the system can continuously release Zn in a pH-responsive manner. 2+ Z@C-MN, along with ChABC, promotes in situ repair of cerebral fibrosis (SCI) after injury. Z@C-MN induces macrophage polarization towards a pro-regenerative phenotype, thereby downregulating the inflammatory response, increasing neurite length, and promoting the differentiation of neural stem cells into neurons. In vivo, Z@C-MN reduced the inflammatory microenvironment after injury and promoted angiogenesis in a rat SCI model. Furthermore, it activated axonal regeneration by inhibiting scar tissue formation and regulating CD4+. + T cell transformation supports neurogenesis.

The invention relates to the field of biological medicine, and discloses application of SCAP as a target spot in treatment or prevention of atherosclerosis. The invention proves that compared with normal control, SCAP expression of macrophages in aortic tissues of atherosclerosis patients is up-regulated. The invention proves that the progress of atherosclerosis can be obviously relieved by macrophage SCAP specific knockout. The invention also proves that macrophage SCAP specific knockout can improve aortic plaque lipid accumulation, and the local inflammation level is improved by influencing macrophage polarization to relieve atherosclerosis. According to the application, the application of the macrophage SCAP as a new target spot in treating or preventing the atherosclerosis is found.

The invention provides a preparation method of dynamic connection bone extracellular matrix simulation hydrogel and application of the dynamic connection bone extracellular matrix simulation hydrogel in bone defect repair, and belongs to the technical field of medical hydrogel. L-cysteine ethyl ester functionalized hyaluronic acid and allicin are prepared to form a thiol-disulfide bond dynamic cross-linked network; a sacrificial pre-vascularization template is constructed by combining a collagen thread loaded with a vascular endothelial growth factor, a vascularization guide microchannel is formed by utilizing the enzymolysis characteristic of collagen, the polarization of macrophages is regulated and controlled by hyaluronic acid to inhibit inflammatory response, and the adhesion and osteogenic differentiation of mesenchymal stem cells are promoted by virtue of a functional surface, so that the osteogenic differentiation of the mesenchymal stem cells is promoted. The synergistic effect of vascularization, anti-inflammation and osseointegration is achieved, finally, the dynamic connection bone extracellular matrix simulation hydrogel with a porous interconnection structure and biological activity is formed, and the technical problems that a traditional bone defect repair material is insufficient in vascularization, inflammation interference exists, and osseointegration is poor are solved.

The invention discloses a preparation method of luteolin-loaded targeted polypeptide nanoparticles and application of the luteolin-loaded targeted polypeptide nanoparticles in hepatic fibrosis treatment, and belongs to the technical field of nano-drug delivery and liver disease treatment. The luteolin-loaded targeted polypeptide nanoparticles are used for treating or relieving liver fibrosis related diseases. According to the nanoparticle disclosed by the invention, a stable structure is formed through self-assembly of the amphiphilic polymer, the luteolin is physically entrapped, targeted delivery of liver immune cells is realized, and the nanoparticle shows good biocompatibility and safety in an in-vivo macrophage polarization and liver fibrosis model; the inflammatory response and collagenous fiber deposition can be remarkably inhibited, and the hepatic tissue fibrosis degree is improved. Compared with the existing anti-hepatic fibrosis medicine which has the problems of poor targeting property, low bioavailability, obvious side effect after long-term medication and the like, the luteolin-loaded targeting polypeptide nanoparticle has the remarkable advantages of high delivery efficiency, strong targeting property, good safety, stable treatment effect and the like.

The present invention provides a silk scaffold material modified with polydopamine and magnesium ions, and its preparation method and application, belonging to the technical field of bone repair materials. The present invention uses hot-pressed flat silk as the base material, eliminating a series of tedious steps such as cocoon peeling and degumming in traditional technologies. The operation is simple, the cost is low, and the process is environmentally friendly. After the flat silk is hot-pressed, the mechanical properties of the silk scaffold material are greatly improved. The present invention uses a ternary solution to micro-treat the silk fiber substrate, thereby increasing the surface roughness of the base material, which is conducive to the subsequent adhesion and growth of cells. Polydopamine formed by dopamine self-polymerization is then grafted onto the material surface, thereby improving the hydrophilicity and biocompatibility of the silk fiber substrate. Magnesium ions can replace the hydroxyl groups in the catechol structure of polydopamine. By utilizing the grafting effect of polydopamine, magnesium ions are grafted onto the surface of the silk fiber substrate, thereby exerting excellent effects in regulating macrophage polarization, promoting angiogenesis, and promoting bone regeneration.

The present invention provides a fiber dressing that promotes wound healing, as well as its preparation method and application, and belongs to the field of biomaterial technology. The outer shell of the fiber dressing of the present invention contains a first drug that promotes macrophage polarization, which can promote the polarization of macrophages from M1 type to M2 type in normal and diabetic wounds; the outer shell matrix is ​​a mixture of hydrophilic and hydrophobic polymers, which can achieve painless adhesion and peeling of the wound by absorbing exudate and spraying non-irritating saline at the wound surface, thereby reducing secondary damage to the wound surface; the inner core contains a second drug and a third drug that induce the death of M1 macrophages, which can reduce the inflammatory response of the wound surface, kill bacteria on the wound surface, reduce the degree of infection on the wound surface, and accelerate wound healing; the inner core matrix is ​​a mixture of hydrophilic and hydrophobic polymers with good biocompatibility, which can provide mechanical support for the fiber dressing and provide a sustained-release effect for the two substances in the inner core. Controlling the composition of the inner core and the outer shell can effectively promote the healing of normal wounds and diabetic wounds.

This invention relates to the field of medical materials technology, providing a hydrogel containing antler mesenchymal stem cell exosomes, its preparation method, and applications. The method includes the following steps: preparation of antler mesenchymal stem cell exosomes; preparation of PVP-HA composite hydrogel; uniformly mixing AMSC-Exos with a hydrogel precursor solution, and forming a hydrogel containing antler mesenchymal stem cell exosomes through physical embedding and non-covalent interactions. This invention utilizes the three-dimensional porous network structure of the hydrogel to achieve long-term sustained release of exosomes, protecting them from rapid degradation; it leverages the unique regenerative advantages of antler-derived exosomes to synergistically promote angiogenesis, inhibit inflammatory responses, and regulate macrophage polarization; it promotes angiogenesis through the miR-486-5p / FGF9 axis, achieving efficient repair of chronic diabetic wounds; and it provides a novel, efficient, safe, and clinically translational strategy for the treatment of chronic diabetic skin lesions.

Disclosed herein is a compound of formula (I): (I) where: A represents a tandem-lock biomarker responsive group; B represents a self-immolative linker; and C represents a chemiluminescent group, or a pharmaceutically acceptable salt or solvate thereof.