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Hydrogel wound dressing with antibacterial and immunoregulation functions, and preparation method

A technology of wound dressing and immune regulation, which is applied in the direction of medical preparations containing active ingredients, antibacterial drugs, local antibacterial agents, etc., and can solve the problems of nanoparticle cytotoxicity, antibiotic resistance, and damage to vital organs, etc.

Active Publication Date: 2021-07-30
BEOGENE BIOTECH GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, nanoparticles loaded with antibiotics have also shown a good preventive effect in anti-infection, but nanoparticles have certain cytotoxicity, and antibiotics are prone to drug resistance, which has obvious damage to vital organs

Method used

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  • Hydrogel wound dressing with antibacterial and immunoregulation functions, and preparation method
  • Hydrogel wound dressing with antibacterial and immunoregulation functions, and preparation method
  • Hydrogel wound dressing with antibacterial and immunoregulation functions, and preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1: the preparation of methacrylated gelatin (GelMA)

[0035] Dissolve 1.5-2.0g of gelatin in 20mL of deionized water, heat and stir in a water bath at 50°C until completely dissolved. After the gelatin is completely dissolved, slowly add 0.8-1.2mL of methacrylic anhydride into the gelatin solution drop by drop with a rubber dropper. Then the mixed solution was magnetically stirred and reacted at a speed of 500-800rpm / min in a water bath at 50°C for 1-4 hours, then transferred to a dialysis bag (molecular weight cut-off of 3500Da), and dialyzed in deionized water for 5 days to remove unreacted of acid anhydride. Finally, centrifuge the reaction solution at a speed of 3000-8000rpm / min for 2-5min, collect the supernatant, freeze it at -80°C, place it in a freeze dryer, and freeze-dry it. The floc obtained is the target product methacrylic acid Melted gelatin (GelMA). The resulting methacrylated gelatin (GelMA) had a degree of substitution of 58%.

[0036] In...

Embodiment 2

[0039] Embodiment 2: Preparation of oxidized dextran (oDex)

[0040] Dissolve 1.0-3.0 g of dextran in 200 mL of deionized water, and stir until completely dissolved. Add 1.0-3.0 g of sodium periodate into the dextran solution under the condition of avoiding light, and react with magnetic stirring at a speed of 500-800 rpm / min at room temperature for 3-4 hours in the dark. Finally, 2 mL of ethylene glycol solution was added to the reaction solution, and stirring was continued for 0.5 to 2 h to remove excess sodium periodate to stop the reaction. After the reaction was completed, the reaction solution was collected and transferred to a cellulose dialysis bag (molecular weight cut off: 3500 Da), and dialyzed in deionized water for 5 days. After dialysis, the solution was frozen at -80°C and placed in a freeze dryer, and the spongy substance obtained after freeze drying was the target product, oxidized dextran (oDex). The degree of oxidation of the obtained oxidized dextran (oDe...

Embodiment 3

[0044] Embodiment 3: the synthesis of black phosphorus nanosheet (BP)

[0045] Weigh 10-30 mg of black phosphorus nanopowder and disperse in 30-60 mL of saturated sodium hydroxide solution of methylpyrrolidone (NMP), and then ultrasonicate the dispersion for 6-12 hours in an ice bath using a cell pulverizer. Centrifuge the turbid solution obtained by ultrasound at a speed of 3000-5000 rpm / min for 5-10 minutes to remove large black phosphorus particles that have not been peeled off, and then collect the upper brown supernatant, which is the dispersion of black phosphorus nanosheets. The method is to prepare black phosphorus nanosheets by using a liquid phase exfoliation method, and the yield is about 12%.

[0046] In the present embodiment, the consumption of the black phosphorus powder is preferably 20 mg; the consumption of the saturated sodium hydroxide solution of the methylpyrrolidone (NMP) is preferably 50 mL;

[0047] The ultrasonic time is preferably 12 hours;

[0048...

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Abstract

The invention relates to the technical field of biomedical engineering materials, and discloses a hydrogel wound dressing with antibacterial and immunoregulation functions, and a preparation method thereof. The hydrogel wound dressing comprises hydrogel, black phosphorus nanosheets loaded in the hydrogel and folic acid modified polyethyleneimine nanoparticles, and the folic acid modified polyethyleneimine nano particles are loaded with MicroRNA-223*. MicroRNA-223* which has a regulation and control function on macrophage polarization is loaded on polyethyleneimine, folic acid modified polyethyleneimine has a better targeting effect on macrophages, black phosphorus nanosheets and polyethyleneimine form nanoparticles due to electrostatic interaction, and the nanoparticles respectively have photodynamic and photothermal effects under the irradiation of near-infrared laser with the wavelength of 660 nm and the wavelength of 808 nm, so that biological membranes and structures of bacteria at the wound surface are destroyed, and the antibacterial effect is achieved; and meanwhile, the nanoparticles release the MicroRNA-223 *, the immune regulation function is played, the wound healing is promoted, and the scar formation is reduced.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to a hydrogel wound dressing with antibacterial and immunoregulatory functions and a preparation method thereof. Background technique [0002] As the largest organ of the human body, the skin can not only maintain metabolism by preventing the evaporation of human body water, but also play an important role in resisting ultraviolet radiation, harmful substances, external factors and other harmful environmental factors. It is the first organ to maintain the normal operation of the human body. a barrier. However, damage to the skin will open loopholes in the body's defense system, such as trauma and burns, which often lead to tissue necrosis and damage the defense function of the skin, thus causing great harm to human health. Bacterial infection is one of the biggest challenges in the wound repair process. The excessive inflammatory response caused by wound infection will...

Claims

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Application Information

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IPC IPC(8): A61L26/00A61K41/00A61P31/04A61P31/02A61P17/02
CPCA61L26/008A61L26/0066A61L26/0004A61L26/0019A61L26/0038A61L26/0023A61L26/009A61L26/0061A61K41/0052A61K41/0057A61P31/04A61P31/02A61P17/02A61L2300/404A61L2300/41A61L2300/412A61L2400/12A61L2300/258A61L2300/602C08L79/02C08L5/02A61K2300/00Y02A50/30
Inventor 郭瑞蓝咏冯龙宝刘玉
Owner BEOGENE BIOTECH GUANGZHOU
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