660results about "Photodynamic therapy" patented technology

The invention discloses an MOF-CQD enzyme-sensitive FRET hydrogel diagnosis and treatment probe as well as a preparation method and application thereof, and belongs to the technical field of biomedical detection and treatment. The probe takes a carboxyl carbon quantum dot (CQD) as a donor and a metal organic framework (MOF) as an acceptor, and the donor and the acceptor are connected through a replaceable enzyme response polypeptide bridge; when the fluorescence is complete, FRET is generated to quench the CQD fluorescence, the FRET is interrupted after the target enzyme cuts off the peptide bridge, and the blue / red fluorescence ratio is changed to realize nanomole-level quantitative detection and has self-correction capability. The probe is packaged in a four-arm PEG transparent hydrogel which does not need a photoinitiator and can be quickly self-crosslinked, and can be prepared into a film, a tooth socket and the like for POCT (point-of-care testing) of parts such as an oral cavity / skin and the like. After detection, the MOF is activated by illumination of 630-660 nm to generate active singlet oxygen, so that local antibacterial or anti-tumor treatment is realized, and a diagnosis and treatment integrated scheme is constructed.

The invention relates to a near-infrared light response in-situ gelling and synergistic photo-thermal-photodynamic gel delivery system and a preparation method and application thereof, a mesoporous silicon coated up-conversion nano-carrier and an efficient photosensitizer are compounded to form a photo-response core unit, and then the photo-response core unit is intelligently integrated with photo-curing hydrogel to form the near-infrared light response in-situ gelling and synergistic photo-thermal-photodynamic gel delivery system. The general problem of aggregation and quenching of photosensitive molecules is relieved by utilizing the unique space confinement effect of the mesoporous structure, the ICG photo-thermal efficiency and photodynamic performance are enhanced, under precise excitation of a near-infrared light source, the system can activate a photo-crosslinking reaction through an up-conversion luminescence process, in-situ conversion from a liquid eye drop preparation to a stable gel state is achieved, and the stability of the eye drop preparation is improved. A lasting drug reservoir is formed on the surface of the cornea; synchronously excited photodynamic and photo-thermal dual-mode treatment generates a remarkable synergistic antibacterial effect, and shows an excellent removal capability on pathogens including highly drug-resistant bacteria; meanwhile, an ideal microenvironment is created for tissue repair through high biocompatibility and a stable mechanical matrix, and integrity recovery of cornea structures and functions is promoted.

The invention belongs to the technical field of organic dye synthesis and application, and discloses a conjugated compound containing a benzothiophenazine structure as well as a preparation method and application thereof, and the conjugated compound containing the benzothiophenazine structure has excellent photophysical properties, singlet oxygen yield and superoxide anion generation capacity. The novel conjugated compounds ENBO-NI and ENBS-NI containing benzothiophenazine structures are successfully prepared by replacing a benzene ring of Nile blue with a cycloalkane structure and adjusting the electron donating ability of an R1 group. The two compounds have near infrared fluorescence emission, good solubility and relatively high ROS yield. Compared with a classical photosensitizer NB, the singlet oxygen yield of ENBO-NI is increased by about 117.65%, and the singlet oxygen yield of ENBS-NI is as high as 70%. The absorption wavelength of the conjugated compound is in a red light wave band. The preparation process is simple and efficient, large-scale production is easy, and the preparation method has a wide clinical application prospect.

The invention relates to the technical field of medical chemistry, in particular to a bridge oxygen site regulatively modified rhodamine anti-cancer fluorescent compound as well as a preparation method and application thereof. The invention develops the rhodamine anti-cancer fluorescent compound of which the meso site is functionalized by tetraarylethene and the bridge oxygen site can be regulated and modified, the fluorescent compound has adjustable red light-near infrared fluorescence emission and excellent fluorescence quantum yield, and the quantum yield can be as high as 99%; the fluorescent compound specifically targets mitochondria, and mainly inhibits growth of tumor cells by inducing para-apoptosis; under irradiation of 655 nm near-infrared laser, ferroptosis is further triggered, efficient treatment of tumor cells is achieved through chemical-photodynamic synergistic effect, and a new treatment thought is provided for overcoming tumor drug resistance.

Method and kit for topical treatment of a skin infection, in particular of superficial skin infections, caused by Staphylococcus aureus. The method comprises the steps of applying a photosensitizer to at least a part of the skin affected by the infection, and subjecting said part of the skin to first photons with a majority energy between 2.8 eV and 3.5 eV at a first energy level; and second photons with a majority energy between 1.24 eV and 1.65 eV at a second energy level, the ratio between the first and the second energy levels being in the range from about 10:1 to 2:1. By the method efficient treatment of Staphylococcus aureus infections can be achieved without the administration of topical antibiotics.

The invention relates to the technical field of antitumor drugs, in particular to a Ru (II) complex and a preparation method and application thereof, and the Ru (II) complex has a structure as shown in a formula I. The Ru (II) complex synthesized by the method disclosed by the invention not only has relatively high cytotoxicity, but also has good photodynamic antitumor activity. After the complex is illuminated, the efficiency of the complex entering cells in an active transportation mode can be accelerated, mitochondria and endoplasmic reticulum are targeted at the same time, mitochondrial membrane potential decline and endoplasmic reticulum stress are caused to form a dual organelle damage effect, immunogenic cell death is caused, the tumor microenvironment is adjusted, and the tumor cell immunogenicity is improved. The enrichment of dendritic cells (DCs) in tumor cells is realized, the chemotactic activity of effector T cells is improved, the proportion of CD4 < + > and Foxp3 < + > cell populations is reduced, and finally the anti-tumor immune response is activated. Meanwhile, the complex provided by the invention can also induce the cell to generate pan apoptosis, retard the cell cycle in the G2 / M period and enhance the effect of the complex in inhibiting tumor proliferation.

Disclosed are an oral hygiene device and an oral hygiene device system, and a method of using the same. The oral hygiene device includes a housing, a battery, a control chip, a light source, and a light guide needle. Battery, control chip and light source are encapsulated in housing to form the oral hygiene device. The housing includes a connector, and light source is positioned in the center of connector. The light source is electrically connected to control chip and battery. The light guide needle is mounted on a head of the housing using a mortise-tenon structure. The light guide needle includes a mounting joint, and is in mortise-tenon connection and in interference fit with the connector through the mounting joint. The oral hygiene device has less damage to teeth or healthy tissue in the oral cavity, and has a good disinfection effect on bacteria in oral cavity.

The invention provides a preparation method of an acoustic / photosensitizer-manganese dioxide nanoparticle and a product thereof. According to the method, firstly, a potassium permanganate reduction method is adopted to precisely synthesize the high-purity MnO2 nano-substrate, and according to the method, the size uniformity and dispersion stability of nano-particles can be precisely controlled by regulating and controlling the pH value and temperature of a reaction system and the concentration of a reducing agent. On the basis, amphiphilic high-molecular polyethylene glycol-polycaprolactone (PEG-PCL) is modified on the surface of the MnO2 nanometre. The whole preparation process is green and mild, and complex equipment is not needed; the reaction conditions are mild, nanoparticles are uniform, and the acoustic / photosensitizer can be combined and applied to an imaging technology to realize real-time monitoring.

The invention relates to the technical field of fluorescent probes, in particular to a cascade activated fluorescent probe as well as a preparation method and application thereof. The fluorescent probe has a structure as shown in a general formula (I): the diagnosis and treatment integrated fluorescent probe is obtained by combining an indoline group with an oxidative dehydrogenation reaction mechanism and an organic functional group with a distorted configuration, so that a near-infrared two-region fluorescent signal can be quickly activated under the action of ROS (reactive oxygen species), and a tumor focus is positioned; the system can be used as a light-activated guiding radar, can selectively illuminate a tumor area by using high space-time controllability of light, activates and amplifies the phototherapy activity according to needs, realizes effective killing of in-vivo and in-vitro tumor cells, and has important significance for precise tumor phototherapy under dual-mode imaging guidance.

The invention belongs to the technical field of biological medicine, and particularly relates to a copper sulfide-gold nano-cluster composite liposome and a preparation method and application thereof, and the copper sulfide-gold nano-cluster composite liposome is composed of copper sulfide nanoparticles, gold nano-clusters and liposome; the preparation method comprises the following steps: loading a gold nano-cluster on the surface of a copper sulfide nano-particle, and then coating the copper sulfide nano-particle with a 1, 2-distearoyl-sn-glycerol-3-phosphorylethanolamine-L-lysine-2, 3-dimethyl maleic anhydride liposome with pH response, so as to obtain the copper sulfide-gold nano-cluster composite liposome. According to the copper sulfide-gold nano-cluster composite liposome prepared based on the method, a biofilm matrix of MRSA can be disintegrated through a photothermal effect, permeation of nanoparticles and ROS is enhanced, and the problem that traditional antibiotics are ineffective to biofilms is solved.

Electrically neutral complex compounds of formula I including any stereoisomer or E / Z isomer thereof wherein: M is Fe < 2 + >, Ru < 2 + >, Os < 2 + >, Co < 3 + >, Rh < 2 + >, Rh < 3 + >, Ir < 3 + >, Ni < 2 + >, Pd < 2 + >, Pt < 2 + > or Pt < 4 + >, in particular Ru < 2 + >; t and T'are H or (C1-C3)-alkyl; lig is a bidentate ligand, in particular 2, 2 '-dipyridyl (bpy) or 4, 7-diphenyl-1, 10-phenanthroline (BPhen); and A is an anion from a pharmaceutically acceptable acid, the electrically neutral complex compounds of formula I are useful in human therapy, in particular as photosensitizers (PS) in photodynamic therapy (PDT) of cancer, skin diseases, fungal infections or microbial infections. They are almost non-toxic under dark conditions at therapeutic doses and exhibit good to excellent photoactivity under hypoxic conditions. They can be used for PDT treatment of deep hypoxia tumors by using light within a far-infrared to near-infrared light treatment window.

The invention belongs to the technical field of biological medicine, and particularly relates to a nano bionic bimetallic MOF and a preparation method and application thereof. The nano bionic bimetallic MOF has a core-shell structure and comprises an inner core and a cell membrane coating the surface of the inner core, the inner core is nano bimetal MOF, and the nano bimetal MOF contains a glucose transport inhibitor and a photosensitizer. Mn / Fe-MOF is synthesized by using a solvothermal method, a glucose transport inhibitor (BAY-876) and a photosensitizer (CyI) are loaded in the Mn / Fe-MOF, and the Mn / Fe-MOF is subjected to cell membrane bionic modification to obtain a core-shell structure which is used for enhancing tumor targeting. The hypoxia microenvironment responds to release oxygen and is actively targeted to a breast cancer tumor site, and the effect of overcoming tumor drug resistance synergistically by multiple mechanisms is verified by depending on a cell / animal model.

This disclosure relates to polyethylene glycol (PEG) -functionalized nanoparticles comprising a functional agent, and preparation methods, properties and applications thereof. The nanoparticle represented by PEG-L-G / P, comprising a type of hydrophilic PEG, a hydrophobic functional agent G, which are covalently linked by L: a linker or a chemical bond, and a type of hydrophobic polymer P. The G and P form the hydrophobic core, while the PEG constitutes the hydrophilic outer layer of the nanoparticle in an aqueous medium. The functional agent comprises one or more functional compounds including a therapeutic drug, an imaging diagnostic agent, a photoelectric responsive diagnostic agent, an immune-stimulating agent, or a combination thereof. The nanoparticles comprising such functional agent can offer various applications in multiple biomedical fields.

The invention discloses a polydopamine-tea polyphenol coated copper manganese oxide composite nano-enzyme as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. According to the invention, a DA-Cu (II)-EGCG (epigallocatechin gallate) complex is formed by virtue of chelation of main components of dopamine and tea polyphenol, namely epigallocatechin gallate, and copper ions; potassium permanganate is introduced for oxidative polymerization to obtain the polydopamine-tea polyphenol / copper manganese oxide organic-inorganic hybrid nano composite material, the nano composite material is monodisperse nano particles, the average particle size is about 80 nm, the nano composite material shows excellent nano enzyme catalytic activity, active oxygen free radicals can be effectively removed, and the nano composite material can be used for preparing nano-enzyme. The material has good photo-thermal conversion performance under near-infrared light, and shows excellent photo-thermal and photodynamic synergistic antibacterial effects. The preparation method disclosed by the invention is simple, mild, environment-friendly, low in cost and convenient for batch production.

The invention discloses a water-soluble near-infrared two-region aggregation-induced emission material and a preparation method and application thereof, and belongs to the technical field of aggregation-induced emission materials.Benzobithiadiazole is used as an electron acceptor group, dithieno-cyclopentadiene and derivatives thereof are used as electron donor units, and benzobithiadiazole and dithieno-cyclopentadiene and derivatives thereof are coupled to form the water-soluble near-infrared two-region aggregation-induced emission material. The organic near-infrared second-region molecule with the intramolecular charge transfer characteristic is constructed. According to the invention, the positively charged quaternary ammonium salt group is introduced into the electron donor group, and the positively charged quaternary ammonium salt group has good hydrophilicity, so that the water solubility of molecules can be effectively improved. Moreover, the water-soluble near-infrared two-region aggregation-induced emission material has excellent photodynamic and photothermal activity, and realizes efficient killing of bacteria (especially propionibacterium acnes) through the synergistic effect of photodynamic and photothermal effects.

The invention relates to the technical field of nano materials and biomedical engineering, and discloses a dye-sensitized rare earth up-conversion luminescent nano material as well as a preparation method and application thereof. The dye-sensitized rare earth up-conversion luminescent nano-material comprises a rare earth doped up-conversion nano-particle core, an unsaturated fatty acid salt self-assembled shell layer and a near-infrared dye modified on the surface of the rare earth doped up-conversion nano-particle. The unsaturated fatty acid salt coating layer endows the material with excellent water dispersibility through a hydrophilic group; a hydrophobic chain segment and an unsaturated double bond can effectively block water and oxygen permeation and quench singlet oxygen, so that the photobleaching half-life period of the material is increased by 86.7 times or more compared with that of a conventional coating material, and the light stability is excellent; the material successfully realizes efficient up-conversion luminescence under excitation of a low-power near-infrared light emitting diode (NIR LED, the power density of which can be as low as 100mW / cm < 2 >), and thoroughly gets rid of dependence on a high-cost and high-risk laser.

A rinsing solution for photodynamic disinfection, in particular oral disinfection, including, at least one photosensitiser from the group comprising riboflavin 5′-phosphate sodium in a proportion of 0.2 to 0.6 wt. % and a photosensitiser other than riboflavin 5′-phosphate sodium in a proportion of 0.001 to 0.05 wt. %, at least one viscosity modulator from the group comprising glycerol in a proportion of 1.0 to 5.0 wt. % and hyaluronic acid sodium salt in a proportion of 0.01 to 0.3 wt. %, at least one biofilm inhibitor from the group comprising xylitol in a proportion of 7.0 to 25.0 wt. %, L-arginine in a proportion of 0.01 to 0.5 wt. % and urea in a proportion of 0.5 to 3.0 wt. %, with the remainder on a water base.

The application discloses an iron / copper-porphyrin nanoparticle and a preparation method and application thereof, and the nanoparticle is prepared through the following steps: adding iron chloride, copper chloride, tetra(4-carboxyphenyl)porphyrin and benzoic acid into a reaction solvent N,N-dimethylformamide solution, uniformly stirring at room temperature, and reacting at high temperature; and after the reaction is completed, the iron / manganese-porphyrin nanoparticle is obtained through centrifugation and washing. The iron / manganese-porphyrin nanoparticle is prepared by using a brand-new method, the nanoparticle can be degraded in a glutathione solution and release iron ions and copper ions, is used for catalyzing hydrogen peroxide to be converted into a hydroxyl radical (•OH), and tetra(4-carboxyphenyl)porphyrin can generate singlet oxygen (O2) under 660 nm laser irradiation, and is expected to become a good nano-carrier for accelerating photodynamic therapy-chemical kinetic therapy. 1 O2).

The application discloses a rare earth nanoparticle-organic photosensitizer composite material and application thereof. By introducing rare earth doped nanoparticles as an energy conversion medium, direct activation of excited triplet states of an organic photosensitizer and sensitized production of singlet oxygen under excitation of radionuclides (e.g., 64Cu, 67Cu, 90Y, 111In, 177Lu, etc.) are realized. 18 F、 99m Tc、 177 Lu, etc.) excitation, the rare earth doped nanoparticles respond to CL and the rare earth ions reach an excited state after various high-energy radiation. Subsequently, through an interface energy transfer process, the excited state energy of the rare earth ions is directly transferred to the excited triplet state of the organic photosensitizer, and then singlet oxygen is generated, realizing tumor cell killing. The nanocomposite material is surface modified to load iRGD peptides to realize in vivo tumor targeting. 18 F-FDG (fluorodeoxyglucose) can realize tumor targeting through abnormal glucose uptake of tumors. The combination of the two realizes double targeting of in vivo tumors, and enhances the anti-tumor efficacy and safety.

The present disclosure relates generally to methods of utilizing sonosensitizers in the presence of thiamine or a derivative thereof for the purpose of cross-linking animal tissue, producing singlet oxygen within or at the site of animal tissue, or treating a condition in a subject, wherein the condition is an aneurysm, cancer, and / or a scleral condition.

The invention discloses a self-aggregation-induced emission type photosensitizer and a preparation method and application thereof. The photosensitizer has excellent AIE performance, and singlet oxygen can be efficiently generated after aggregation in a physiological environment; and the photosensitizer can effectively overcome the aggregation-induced quenching phenomenon, and has a synergistic effect with the lovir drugs, so that the anti-herpes virus activity is remarkably improved, and the dosage and side effects are reduced.

A process for preparing a supramolecular carrier photosensitizer for photodynamic therapy and a formulation thereof is disclosed for prevention, treatment, anti-inflammatory and sterilization of oral dental diseases. Supramolecular carrier consists of refined lignin, glucomannan, cysteine, and sodium benzoate at a mass ratio of 0.1-1.0%: 0.5-3%: 0.1-0.5%: 0.05%, and the balance of water. Aqueous solutions of refined lignin, glucomannan, cysteine and sodium benzoate are prepared, and a pH value of combined solution is adjusted to 6.5, so that dissolved alkali lignin is precipitated to form an efficient and stable three-dimensional polymer network skeleton, and rich hydrogen bonds are facilitated to be formed between the same molecules and different molecules of lignin and glucomannan, thereby forming a supramolecular carrier formulation loaded with the photosensitizer. It ensures long-term storage stability of physicochemical traits of formulation, which is conducive to rapidly promoting application of PDT in anti-inflammatory and sterilization treatment of oral dental diseases.

The application discloses a preparation method of a tumor magnetic targeting guided magnetic heat self-enhancing nano diagnosis and treatment agent, and belongs to the technical field of cancer diagnosis and treatment. The preparation method comprises the following steps: S1, preparing NaGdF4:Nd / Yb Tm nanoparticles; and S2, preparing NaGdF4:Nd / Yb Tm@WFe2O4-NH2-PEG magnetic nanoparticles. The tumor magnetic targeting guided magnetic heat self-enhancing nano diagnosis and treatment agent provided by the application has good dispersity, strong magnetic attraction, can be efficiently enriched at a tumor site under magnetic guidance, can generate a large amount of heat in an alternating magnetic field to realize MHT treatment of the tumor, has near-infrared II region fluorescence imaging and MRI dual-mode imaging effects, thereby improving the diagnosis and treatment accuracy of the tumor and realizing diagnosis and treatment integration, has high biological safety and small toxic and side effects, and the preparation method is simple and easy to operate.

The present application relates to an antifungal microneedle patch and its preparation method and application, the antifungal microneedle patch includes backing layer and the needle head arranged on the surface of the backing layer;The needle head constitutes n×n array on the surface of the backing layer;The surface of the needle head includes fatty acid coating containing antibacterial drug;The inside of the needle head includes photosensitizer;The n is selected from integer.The antibacterial drug is lipophilic triazole antifungal drug.The fatty acid is selected from saturated fatty acid containing 6-12 carbon atoms.The present application innovatively combines antifungal drug itraconazole and photo-thermal agent / photosensitizer indocyanine green, loads in microneedle, can carry out painless minimally invasive local administration, through the antibacterial action, photo-thermal effect and photodynamic effect of antifungal drug released under the excitation of near-infrared light, realize the effective killing to fungi, and will not cause irreversible damage to normal tissue.

The invention discloses a mitochondrial targeting and apoptosis-inducing photosensitive iridium complex and a preparation method thereof, and particularly discloses a 2, 2 '-bipyridine compound, a cyclometalated iridium complex using the compound as a ligand, a preparation method and application of the 2, 2'-bipyridine compound as an antitumor drug. The cyclometalated iridium complex provided by the invention takes a 2, 2 '-bipyridine compound as a ligand, can selectively induce tumor cell apoptosis under an illumination condition, can be used for preparing antitumor drugs, and has a great potential value in the field of photodynamic treatment of tumors.

The present invention discloses a near-infrared photosensitizer-based nanotherapeutic agent, its preparation method, and application. The near-infrared photosensitizer-based nanotherapeutic agent comprises a protein and a near-infrared prodrug bound to a hydrophobic cavity of the protein. The near-infrared prodrug comprises a near-infrared photosensitizer connected to a cupric sulfide bond and a chemotherapy drug bound to the near-infrared photosensitizer connected to the cupric sulfide bond via a thioketone bond. The near-infrared photosensitizer-based nanotherapeutic agent provided by the present invention significantly improves the solubility and bioavailability of the chemotherapy drug and the near-infrared photosensitizer, achieves tumor targeting and selectivity, thereby increasing the drug accumulation at the tumor site, improving the therapeutic effect, and reducing toxic side effects. This solves the problems of existing near-infrared fluorescent dyes, which have limited targeting and selectivity and low therapeutic effect.

The invention relates to an estrogen receptor alpha and fibroblast activating protein dual-targeting near-infrared fluorescent probe as well as a preparation method and medical application thereof. Specifically, the probe can be used as a fluorescence probe for developing cells co-expressing the estrogen receptor alpha and the fibroblast activating protein, and can also be used as a near-infrared fluorescence imaging probe for diagnosing benign / malignant lesions such as nodules / tumors co-expressing the estrogen receptor alpha and the fibroblast activating protein. The fluorescent probe can also be applied to research on related biological events in cells for co-expressing the estrogen receptor alpha and the fibroblast activating protein, and particularly can be applied to fluorescent navigation in tumor resection operations for co-expressing the estrogen receptor alpha and the fibroblast activating protein; particularly, the composition can be applied to the synergistic treatment of an anti-estrogen therapy and a photodynamic therapy on a diseased region which co-expresses the estrogen receptor alpha and the fibroblast activating protein.

The present invention relates to a composition comprising an association of stabilizing compounds including ethylenediaminetetraacetic acid (EDTA) and / or one or more salts thereof, histidine, and sodium chloride, and indocyanine green.

The invention belongs to the field of anti-cancer drugs, and particularly relates to an anti-cancer porphyrin molecular cage structure and cytotoxic application of the anti-cancer porphyrin molecular cage structure to liver cancer. The invention discloses two coordination molecular cages Pd-TMPP (Cu) and Pd-TMPP (Zn), and detailed and accurate verification is carried out on the structural characteristics and element composition of the two coordination molecular cages through a series of tests. And then the two compounds are modified by hyaluronic acid so as to obtain targeting and water solubility. Experimental results show that the Pd-TMPP (Zn) has concentration-dependent cytotoxicity to HuH-7, Hep-G2 and PLC / PRF / 5, and the cytotoxicity is remarkably enhanced after the Pd-TMPP (Zn) is irradiated by infrared light.