29 results about "Tissue scaffolds" patented technology

Some embodiments provide methods for forming self-supporting, three-dimensional structure by extrusion of a fiber-hydrogel composite material that is solid prior to extrusion, liquifies under stress during extrusion, and resolidifies after extrusion to form the self-supporting, three-dimensional structure. No support bath, no coagulating bath and no cross-linking bath are used during extrusion of the fiber-hydrogel composite material to form the three-dimensional self-supporting structure. In some embodiments, the self-supporting, three-dimensional structure is a tissue scaffold. In some embodiments, the resulting self-supporting three-dimensional structure is edible.

This invention discloses a UV-crosslinked regenerated silk fibroin hydrogel fiber, its preparation method, and its applications. Using silk fibroin as the main raw material, the silk fibroin is first regenerated to obtain regenerated silk fibroin. Then, the regenerated silk fibroin fibers are collected using electrospinning and a rotating collection roller. These fibers are then soaked in a solution containing a photoinitiator and placed under UV light for crosslinking to form regenerated silk fibroin hydrogel fibers. Finally, the fibers are washed and soaked to obtain the regenerated silk fibroin hydrogel fiber. The advantages are: good mechanical strength and softness, suitable for use as wound dressings or tissue scaffolds; high water content and good swelling properties, capable of absorbing wound exudate; the hydrogel fiber membrane has good drug release performance, can be loaded with drugs for antibacterial or wound repair promotion; it has excellent biocompatibility and mechanical properties, and has broad application prospects as a drug carrier and tissue repair material in tissue engineering and drug release fields.

Provided is an implantable tissue scaffold comprising a mixture of a biocompatible organic polymer and chitin, wherein the chitin is embedded in the biocompatible organic polymer. Also provided is a composition for 3D printing, the composition comprising at least one biocompatible organic polymer and chitin that may be partially deacetylated, wherein the chitin is embedded within the biocompatible organic polymer. Further provided is a method of promoting tissue formation, comprising implanting a tissue scaffold comprising a mixture of a biocompatible organic polymer and chitin at a site in need of regenerative bone tissue formation.

The present disclosure provides tissue scaffolds and hydrogels as well as methods of making and using the same. Also provided are tissue scaffolds with improved biological properties and methods of utilizing said tissue scaffolds for the treatment of wounds.

Provided herein is a tissue scaffold that may include a microstructure pattern on one or more surfaces that may alter physical properties of the tissue scaffold (118). The microstructure pattern may be cell-directed over a larger spatial range compared to the prior art using a chemically modified substrate. The present disclosure also includes a tissue scaffold configured such that cells may permeate the tissue scaffold through a pattern responsive to surface energy gradients on the tissue scaffold, and cells may not be constrained within the pattern by physical means. When the cells proliferate, they associate and orient in response to long-range patterns to form confluent monolayer cells while constructing functional macrostructures across the tissue scaffold surface.

The invention relates to a tissue scaffold developed for use in the treatment of cartilage tissue loss and a method for its production.

According to some embodiments, a septum closure device includes a support structure including a first anchor portion and an opposing second anchor portion, with a lumen extending through a center of the first anchor portion and a center of the second anchor portion, the support structure being configured to contract and expand between a compressed tubular configuration for insertion through a patient's vasculature and an expanded configuration in which the first and second anchor portions extend radially outward from the lumen; and a membrane coupled to the first anchor portion, the membrane configured to occlude a majority of the lumen when the support structure is expanded, the membrane configured to promote tissue growth at least across the membrane.

The invention provides a dental pulp regeneration structure based on a piezoelectric intelligent material and a preparation method of the dental pulp regeneration structure, and relates to the technical field of dental pulp regeneration, the preparation method of the dental pulp regeneration structure based on the piezoelectric intelligent material comprises the following steps: mixing a biodegradable polymer and piezoelectric ceramic in chloroform to form uniform slurry, the method comprises the following steps: constructing a three-dimensional porous scaffold with periodically arranged pores by adopting a 4D printing method, wherein the Poisson ratio of the three-dimensional porous scaffold is-0.3 to-0.8; and a piezoelectric material film is attached to the surface of the three-dimensional porous bracket. The tissue scaffold forming the three-dimensional space complex has a negative Poisson's ratio superstructure design, the energy absorption effect can be remarkably improved, the piezoelectric property can be enhanced, the differentiation efficiency of tissue cell nerves can be improved through stronger electrical stimulation, and dental pulp regeneration is promoted.

The present invention relates to PCL / GelMA (polycaprolactone / gelatin methacrylate) tissue scaffolds cultured with HUC-MSCs (human umbilical cord mesenchymal stem cells), a method for their production, and their use in pelvic floor reconstruction. Experimental results demonstrating the efficacy of the tissue scaffolds obtained according to the invention are also presented.

The disclosure relates to biomaterials, specifically non-pyrogenic bacterial cellulose for implantable medical applications, and to a depyrogenation process. It addresses the problem that sterile bacterial cellulose remains pyrogenic due to endotoxin, particularly lipopolysaccharide, which resists standard sterilization and purification. The process involves providing a purified bacterial cellulose membrane in an aqueous medium and subjects it to depyrogenation at about 140°C–250°C under pressure sufficient to maintain the aqueous phase liquid. The treated material is a bacterial cellulose hydrogel having a water holding capacity of at least 100 g water / g dry bacterial cellulose and endotoxin below 0.5 EU / mL as determined by a Limulus amebocyte lysate assay on enzymatically degraded samples. The product and process are useful for manufacturing non-pyrogenic implantable bacterial cellulose devices, including internal implants, tissue scaffolds, and other biocompatible medical components.

According to some embodiments, an interseptal occluding device comprises a support structure comprising a first anchoring portion and an opposite second anchoring portion, a lumen extending through a center of the first anchoring portion and a center second anchoring portion, wherein the support structure is configured to contract and expand between a compressed tubular configuration for insertion through a patient's vasculature, and an expanded configuration, in which the first and second anchoring portions extend radially outwards from the lumen; and a membrane coupled to the first anchoring portion, the membrane configured to occlude a majority of the lumen when the support structure is expanded, the membrane configured to promote tissue growth at least across the membrane.

The application discloses a kind of iodine-loaded antibacterial starch nanofiber membrane and preparation method thereof.The method is mixed and handled after starch, strong alkali and ethanol aqueous solution, and then the precipitate is dissolved in water to prepare starch solution, and the starch electrospinning solution is obtained after high-temperature steam cooking, then electrospinning is prepared starch nanofiber membrane, finally solid-phase adsorption iodine vapor to obtain starch-iodine nanofiber membrane.The average fiber diameter of the starch-iodine nanofiber membrane obtained by the application is 127-141 nm, the iodine content is 11.43%-17.00%, and the antibacterial efficiency of staphylococcus aureus and salmonella is highly sensitive, with high antibacterial efficiency, which can be applied to antibacterial wound dressings, tissue scaffolds and food packaging and other fields.

Systems and methods for modeling contraction deflection of a flexible tissue scaffold are disclosed herein. The systems and methods include obtaining images of a flexible stent at a plurality of points in time, wherein the flexible stent is depicted to deflect along a first dimension due to contraction forces exerted thereon at the plurality of points in time. Curves are fitted to the images such that each curve extends along a centerline of the flexible stent within the respective image. Displacement values are determined from the curves that provide measurements along the first dimension between respective curves and a reference line extending along a second dimension perpendicular to the first dimension. A model may then be generated based on the displacement values. The model characterizes contraction forces exerted on the flexible stent over the plurality of points in time.

Tissue scaffolds for neural tissue growth have a plurality of microchannels disposed within a sheath. Each microchannel comprises a porous wall having a thickness of ≤about 100 μm that is formed from a biocompatible and biodegradable material comprising a polyester polymer. The polyester polymer may be polycaprolactone, poly(lactic-co-glycolic acid) polymer, and combinations thereof. The tissue scaffolds have high open volume % enabling superior (linear and high fidelity) neural tissue growth, while minimizing inflammation near the site of implantation in vivo. In other aspects, methods of making such tissue scaffolds are provided. Such a method may include mixing a reduced particle size porogen with a polymeric precursor solution. The material is cast onto a template and then can be processed, including assembly in a sheath and removal of the porogen, to form a tissue scaffold having a plurality of porous microchannels.

A method and apparatus for degrading a hypodermal tissue scaffold involves exposing the scaffold to a low-intensity focused ultrasonic beam such as to burst gas vesicles trapped in the silk fibroin from which the scaffold is constructed. The rate of degradation can be controlled using ultrasonic beams of varying intensities. In one embodiment, the ultrasonic beams are administered trans-dermally to a hypodermal scaffold.

The present application belongs to the field of medical preparations, and particularly relates to an ultrasonic imaging hydrogel, a preparation method and application thereof. The present application adds imaging particles to the hydrogel system, so that the system has good ultrasonic imaging effect. Meanwhile, hydroxymethyl cellulose is specifically selected as a suspending agent, which not only significantly improves the stability of the imaging particles in the system, reduces the sedimentation of the imaging particles, but also is beneficial to maintaining the rapid gelation performance of the hydrogel, has great industrialization value and clinical use value, and can provide new materials for the fields of medicine and tissue engineering, and be applied to implantable medical devices, permanent embolization, artificial tissue scaffolds and the like.

The invention discloses a decellularized extracellular matrix modified flexible mesh scaffold for achilles tendon rupture repair and a preparation method thereof, and belongs to the technical field of degradable tissue scaffolds. The stent provided by the invention adopts a double-layer composite structure design, and the outer layer is a PCL / ECM gel mixed electrostatic spinning layer and loads natural growth factors; a degradable PCL hollow support is printed on the inner layer in a fused deposition 3D mode and used for providing mechanical support for a main body. According to the stent, a bioactive layer is constructed, natural slow release of growth factors is achieved, uncontrollability of exogenous addition is avoided, the tendon regeneration induction capacity is remarkably enhanced, the achilles tendon repair capacity is preliminarily verified in a rabbit achilles tendon injury model, an ECM / PCL degradable material is adopted, secondary operation is avoided, and the stent has excellent medical application prospects.

Provided herein is a method for producing a tissue graft. The graft may include a living cell and an attachment scaffold. The method may include rinsing a tissue sample; incubating the sample in a hypertonic salt solution to produce a hypertonic solution-treated tissue sample; incubating the treated tissue sample with an ionic detergent to produce a detergent-treated tissue sample; incubating the detergent-treated sample with a DNase to produce a DNase-treated scaffold; incubating the DNase-treated scaffold with a virus-inactivating compound to produce a virus- inactivated scaffold; and contacting the virus-inactivated scaffold with a living cell population to produce a tissue graft.

This specification provides a tissue scaffold (118) which may include one or more surface microstructure patterns that can alter the physical properties of the tissue scaffold. The microstructure patterns may be cell-directed over a larger spatial range than in the prior art using chemically modified substrates. The disclosure further includes a tissue scaffold configured such that cells can infiltrate the tissue scaffold by responding to a pattern of surface energy gradients on the tissue scaffold, and the cells cannot be constrained to the pattern by physical means. As the cells proliferate, they associate and orient themselves in response to the long-range pattern to form a confluent monolayer of cells, constructing a functional macrostructure across the surface of the tissue scaffold.

Devices and methods for treating an intraoral cavity of a patient are provided. In some embodiments, a device includes a tissue scaffold configured to promote growth of oral tissue at a treatment site in the intraoral cavity of the patient, and a positioner. The positioner can include one or more cavities configured to receive one or more teeth of the patient, and an alignment element configured to temporarily couple to the tissue scaffold such that when the positioner is placed on the patient's teeth, the alignment element locates the tissue scaffold at the treatment site.

The invention relates to a 3D tissue scaffold containing the active pharmaceutical ingredients, memantine hydrochloride and donepezil hydrochloride, and a method of production of this tissue scaffold. Said scaffold accelerates bone repair thanks to the active pharmaceutical ingredients contained therein and a 3D porous structure.

The application provides an application of an algal composition in plant protein meat and belongs to the technical field of food processing; the carotenoid content in the plant protein meat can be regulated, and a preparation method comprises the following steps: mixing algal cells and a tissue scaffold matrix to obtain a bio-ink, performing 3D printing to obtain an algal composition, cross-linking by using a cross-linking agent, and performing high-density culture and stress induction culture after forming a hydrogel. In the application, microalgae are used as a sustainable nutrient source and an endogenous colorant, and the application first realizes the regulation of microalgae-derived pigments in cultured meat, establishes a low-carbon and reasonable research framework for future food system design customization and obtaining of health-promoting cultured meat products.

The invention belongs to the technical field of fiber materials, and discloses a controllable nano-microporous structure fiber material based on thermoplastic polyvinyl alcohol blending and a preparation method and application thereof. The controllable nano-microporous structure fiber is prepared through melt spinning and subsequent water-soluble induction treatment, selective dissolution of TPVA is achieved, a composite system is induced to be subjected to microphase separation and structural rearrangement, and therefore nano-to-micron pore structures which are evenly distributed are constructed on the surface and inside the fiber. The obtained fiber has high specific surface area, large porosity and good pore connectivity, shows excellent adsorption and filtration performance, is suitable for functional application fields of liquid separation, gas purification, volatile organic compound trapping, oil-water separation, tissue scaffolds and the like, breaks through the dependence on a typical island interface structure, and has wide application prospects. The design space and the process adaptability of the thermoplastic PVA-based composite fiber are obviously widened, and the thermoplastic PVA-based composite fiber has good machinability, structural adjustability and industrial application prospects.

The present disclosure provides tissue scaffolds and hydrogels as well as methods of making and using the same. Also provided are tissue scaffolds with improved biological properties and methods of utilizing said tissue scaffolds for the treatment of wounds.

A packaging system (10b) includes a package defining a cavity (14b) and a seal (18b) configured to be coupled to the package to fluidly seal the cavity. The packaging system can also include at least one tissue scaffold (16b) configured to be disposed within the cavity. Cavities having a single size can be configured to receive tissue scaffolds having different sizes. In addition to the cavity, the packaging system can include one or more additional reservoirs configured to hold a quantity of liquid. Such packaging systems can be used to store human or animal tissue for wet or dry storage. The invention also provides a packaged tissue sample and methods of packaging tissue.

The present disclosure relates to a three-dimensionally (3D) printed tissue engineering scaffold for tissue regeneration and a method for manufacturing the 3D printed tissue engineering scaffold. The 3D printed tissue engineering scaffold may be fabricated at least in part from a composite material having an insoluble component and soluble component. The three-dimensional tissue scaffolds of the disclosure may be fabricated via a rapid prototyping machine. In some instances, the three-dimensional shape of the fabricated tissue engineering scaffold may correspond to a three-dimensional shape of a tissue defect of a patient.

The application provides a 4D printed bone tissue scaffold with adjustable microenvironment and a preparation method and application thereof, and relates to the technical field of bone repair. The 4D printed bone tissue scaffold with adjustable microenvironment comprises a scaffold structure and a film covering the surface of the scaffold structure, and the scaffold structure is provided with holes; under the condition that the scaffold structure is deformed under stress, the holes are configured to switch between a contact state and a separation state. During the deformation of the scaffold structure under stress, the holes can switch between the contact state and the separation state, generate a triboelectric effect, and further generate a micro-current, so as to construct a physical microenvironment conducive to cell growth, and further conducive to the regeneration of damaged bone tissue; as a mechanical wave, ultrasound provides additional mechanical stimulation at the bone defect site, can make the scaffold structure deform under stress, and can also make the scaffold structure generate a piezoelectric effect and a triboelectric effect, induce the generation of a micro-current on the surface of the scaffold structure, and thus construct a physical microenvironment conducive to cell growth, and further conducive to the regeneration of damaged bone tissue.

Methods for the development and integration of multiple apparatuses and methods for achieving administration of stem cell therapies include precision manufacturing of tissue scaffolds and / or bioreactor substrates. The nano / microscale fiber material extrusion typifying the electrospinning process is married with the fiber alignment and layering characteristic of an additive manufacturing process. The method generates porous fibrous 3-D meshes with precision controlled structures from biopolymer melts and solutions and gels, blends, and suspensions with and without cells. A method of tracking the migration histories and shapes of stem cells on scaffold surfaces relies on immunofluorescent imaging and automated algorithms based on machine learning. The combination of the precision manufacturing method and the method of cell tracking and cell shape statistics, along with understanding of the intimate relationship between the cell shape / phenotype and scaffold architecture leads to an integrated method for cultivating and harvesting cells having desired phenotypes.

The present invention relates to methods for preparing a crease patterned fibre article, a crease patterned fibre article obtainable by said method as well as the use of a crease patterned fibre article as a packaging, design article, fabrics, bio-tissue implant, organ substitute, organ regeneration (patch), organ model, tissue substitute, tissue model, tissue scaffolds, cell growth support, cell maturing support or bone reinforcement.