51 results about "Tissue scaffolds" patented technology

The invention discloses an engineered autologous tumor tissue scaffold and application thereof, and the engineered autologous tumor tissue scaffold is prepared by performing specific treatment on autologous tumor tissue to induce immunogenic cell death of the tumor tissue, and then sequentially performing freezing and drying steps; the engineered autologous tumor tissue scaffold can be used for loading dendritic cells and constructing personalized tumor vaccines. According to the stent, autologous tumor tissue is adopted, so that a specific and comprehensive tumor antigen pedigree is reserved, and multi-epitope immunostimulation is provided; and meanwhile, excellent biocompatibility is ensured, the immunological rejection risk is reduced, and relatively high safety is ensured.

The invention relates to a heat and light sensitive tissue scaffold suitable for the growth, proliferation and organization of the cells in the technical field of tissue engineering, which is endowed with intelligent behavior and thus has a dynamic structure, and a method for producing the same.

Some embodiments provide methods for forming self-supporting, three-dimensional structure by extrusion of a fiber-hydrogel composite material that is solid prior to extrusion, liquifies under stress during extrusion, and resolidifies after extrusion to form the self-supporting, three-dimensional structure. No support bath, no coagulating bath and no cross-linking bath are used during extrusion of the fiber-hydrogel composite material to form the three-dimensional self-supporting structure. In some embodiments, the self-supporting, three-dimensional structure is a tissue scaffold. In some embodiments, the resulting self-supporting three-dimensional structure is edible.

Disclosed herein are silk fibroin composite matrixes as scaffolds in tissue regeneration and the process for preparation thereof.

A fabric or mesh construct, and process for making same, which allows for early wound stability and then transitions to a more compliant state exhibiting a substantially constant macro-porous pore structure through the life of the implant to promote good tissue incorporation without bridging.

A surgical tool kit and a method of installing a tissue, the method comprising positioning an alignment guide to ensure proper alignment of surgical tools relative to a lesion in a host tissue; inserting a guide pin into the host tissue to provide a reference point; using a punch to score the host tissue at the location of the guide pin; utilizing a cannulated reamer to prepare a socket in the host tissue; evaluating the prepared socket in the host tissue with a trial implant to ensure proper fit and alignment; employing an undercut tool to prepare at least one groove in the host tissue; and placing the tissue scaffold into the prepared socket using an insertion tool.

This invention discloses a UV-crosslinked regenerated silk fibroin hydrogel fiber, its preparation method, and its applications. Using silk fibroin as the main raw material, the silk fibroin is first regenerated to obtain regenerated silk fibroin. Then, the regenerated silk fibroin fibers are collected using electrospinning and a rotating collection roller. These fibers are then soaked in a solution containing a photoinitiator and placed under UV light for crosslinking to form regenerated silk fibroin hydrogel fibers. Finally, the fibers are washed and soaked to obtain the regenerated silk fibroin hydrogel fiber. The advantages are: good mechanical strength and softness, suitable for use as wound dressings or tissue scaffolds; high water content and good swelling properties, capable of absorbing wound exudate; the hydrogel fiber membrane has good drug release performance, can be loaded with drugs for antibacterial or wound repair promotion; it has excellent biocompatibility and mechanical properties, and has broad application prospects as a drug carrier and tissue repair material in tissue engineering and drug release fields.

System and methods for modelling contractile deflection of flexible tissue scaffolds are disclosed herein. The system and methods comprise obtaining images of a flexible scaffold at a plurality of time points, where the flexible scaffold are depicted as deflected along a first dimension due to contractile forces exerted thereupon at the plurality of time points. Curves are fit to the images such that each curve extends along a centerline of the flexible scaffold within a respective image. Displacement values are determined from the curves, which provides a measurement along the first dimension between a respective curve and a reference line extending along a second dimension perpendicular to the first dimension. A model may then be generated based on the displacement values. The model characterizes contractile forces exerted on the flexible scaffold over the plurality of time points.

Provided is an implantable tissue scaffold comprising a mixture of a biocompatible organic polymer and chitin, wherein the chitin is embedded in the biocompatible organic polymer. Also provided is a composition for 3D printing, the composition comprising at least one biocompatible organic polymer and chitin that may be partially deacetylated, wherein the chitin is embedded within the biocompatible organic polymer. Further provided is a method of promoting tissue formation, comprising implanting a tissue scaffold comprising a mixture of a biocompatible organic polymer and chitin at a site in need of regenerative bone tissue formation.

The present disclosure provides tissue scaffolds and hydrogels as well as methods of making and using the same. Also provided are tissue scaffolds with improved biological properties and methods of utilizing said tissue scaffolds for the treatment of wounds.

Provided herein is a tissue scaffold that may include a microstructure pattern on one or more surfaces that may alter physical properties of the tissue scaffold (118). The microstructure pattern may be cell-directed over a larger spatial range compared to the prior art using a chemically modified substrate. The present disclosure also includes a tissue scaffold configured such that cells may permeate the tissue scaffold through a pattern responsive to surface energy gradients on the tissue scaffold, and cells may not be constrained within the pattern by physical means. When the cells proliferate, they associate and orient in response to long-range patterns to form confluent monolayer cells while constructing functional macrostructures across the tissue scaffold surface.

The invention relates to a tissue scaffold developed for use in the treatment of cartilage tissue loss and a method for its production.

The invention relates to a magnetic substrate based tissue scaffold suitable for use in the growth, proliferation, and organization of cells in the technical field of tissue engineering, provided with intelligent behavior characteristics and thus dynamic structure, and a method for the production of this tissue scaffold with the support of a 3D printer.

The invention provides a system and method for achieving adjustable rigidity of a tissue scaffold based on magnetorheological fluid, and relates to the technical field of intelligent biomedical engineering.The system comprises a scaffold module, a sensing feedback module, a control center module, a magnetic field module and a user interaction module.The magnetic response microchannel tissue scaffold is prepared through 3D printing and a photoetching machine; the method comprises the following steps: acquiring a mechanical state and an environment signal of a magnetic response micro-channel tissue scaffold, processing to obtain mechanical state and environment data, generating a PWM pulse signal through an LSTM model and a self-adaptive PID algorithm, generating a controllable magnetic field according to the PWM pulse signal, adjusting the rigidity of magnetorheological fluid according to the PWM pulse signal, adjusting parameters of the self-adaptive PID algorithm through a parameter display adjustment area, and meanwhile, adjusting the rigidity of the magnetorheological fluid according to the controllable magnetic field. And displaying the mechanical state data and the environmental data through a data display area. According to the system, through cooperative work of all the modules, precise regulation and control of the rigidity of the tissue scaffold are achieved, and the system has important application value in the field of biomedical engineering.

A method for micro-tissue encapsulation of cells includes coating a tissue scaffold stamp with an extracellular matrix compound; depositing the tissue scaffold stamp onto a thermoresponsive substrate; seeding the tissue scaffold stamp with a cell culture; incubating the cell culture on the tissue scaffold stamp at a temperature that is specified, wherein the cell culture forms a cell patch that is attached to the extracellular matrix compound; removing the thermoresponsive substrate by lowering the temperature; removing the tissue scaffold stamp from the cell patch to form a micro-tissue structure by dissolving the tissue scaffold stamp in a solvent; folding the micro-tissue structure by suspending the micro-tissue in the solvent to enable the cell patch to fold the micro-tissue structure; collecting the folded micro-tissue structure from the solvent; and administering the folded micro-tissue structure to an organism.

According to some embodiments, a septum closure device includes a support structure including a first anchor portion and an opposing second anchor portion, with a lumen extending through a center of the first anchor portion and a center of the second anchor portion, the support structure being configured to contract and expand between a compressed tubular configuration for insertion through a patient's vasculature and an expanded configuration in which the first and second anchor portions extend radially outward from the lumen; and a membrane coupled to the first anchor portion, the membrane configured to occlude a majority of the lumen when the support structure is expanded, the membrane configured to promote tissue growth at least across the membrane.

The invention provides a dental pulp regeneration structure based on a piezoelectric intelligent material and a preparation method of the dental pulp regeneration structure, and relates to the technical field of dental pulp regeneration, the preparation method of the dental pulp regeneration structure based on the piezoelectric intelligent material comprises the following steps: mixing a biodegradable polymer and piezoelectric ceramic in chloroform to form uniform slurry, the method comprises the following steps: constructing a three-dimensional porous scaffold with periodically arranged pores by adopting a 4D printing method, wherein the Poisson ratio of the three-dimensional porous scaffold is-0.3 to-0.8; and a piezoelectric material film is attached to the surface of the three-dimensional porous bracket. The tissue scaffold forming the three-dimensional space complex has a negative Poisson's ratio superstructure design, the energy absorption effect can be remarkably improved, the piezoelectric property can be enhanced, the differentiation efficiency of tissue cell nerves can be improved through stronger electrical stimulation, and dental pulp regeneration is promoted.

Tissue scaffolds for neural tissue growth have a plurality of microchannels disposed within a sheath. Each microchannel comprises a porous wall having a thickness of ≤about 100 μm that is formed from a biocompatible and biodegradable material comprising a polyester polymer. The polyester polymer may be polycaprolactone, poly(lactic-co-glycolic acid) polymer, and combinations thereof. The tissue scaffolds have high open volume % enabling superior (linear and high fidelity) neural tissue growth, while minimizing inflammation near the site of implantation in vivo. In other aspects, methods of making such tissue scaffolds are provided. Such a method may include mixing a reduced particle size porogen with a polymeric precursor solution. The material is cast onto a template and then can be processed, including assembly in a sheath and removal of the porogen, to form a tissue scaffold having a plurality of porous microchannels.

The present invention relates to PCL / GelMA (polycaprolactone / gelatin methacrylate) tissue scaffolds cultured with HUC-MSCs (human umbilical cord mesenchymal stem cells), a method for their production, and their use in pelvic floor reconstruction. Experimental results demonstrating the efficacy of the tissue scaffolds obtained according to the invention are also presented.

The disclosure relates to biomaterials, specifically non-pyrogenic bacterial cellulose for implantable medical applications, and to a depyrogenation process. It addresses the problem that sterile bacterial cellulose remains pyrogenic due to endotoxin, particularly lipopolysaccharide, which resists standard sterilization and purification. The process involves providing a purified bacterial cellulose membrane in an aqueous medium and subjects it to depyrogenation at about 140°C–250°C under pressure sufficient to maintain the aqueous phase liquid. The treated material is a bacterial cellulose hydrogel having a water holding capacity of at least 100 g water / g dry bacterial cellulose and endotoxin below 0.5 EU / mL as determined by a Limulus amebocyte lysate assay on enzymatically degraded samples. The product and process are useful for manufacturing non-pyrogenic implantable bacterial cellulose devices, including internal implants, tissue scaffolds, and other biocompatible medical components.

According to some embodiments, an interseptal occluding device comprises a support structure comprising a first anchoring portion and an opposite second anchoring portion, a lumen extending through a center of the first anchoring portion and a center second anchoring portion, wherein the support structure is configured to contract and expand between a compressed tubular configuration for insertion through a patient's vasculature, and an expanded configuration, in which the first and second anchoring portions extend radially outwards from the lumen; and a membrane coupled to the first anchoring portion, the membrane configured to occlude a majority of the lumen when the support structure is expanded, the membrane configured to promote tissue growth at least across the membrane.

The application discloses a kind of iodine-loaded antibacterial starch nanofiber membrane and preparation method thereof.The method is mixed and handled after starch, strong alkali and ethanol aqueous solution, and then the precipitate is dissolved in water to prepare starch solution, and the starch electrospinning solution is obtained after high-temperature steam cooking, then electrospinning is prepared starch nanofiber membrane, finally solid-phase adsorption iodine vapor to obtain starch-iodine nanofiber membrane.The average fiber diameter of the starch-iodine nanofiber membrane obtained by the application is 127-141 nm, the iodine content is 11.43%-17.00%, and the antibacterial efficiency of staphylococcus aureus and salmonella is highly sensitive, with high antibacterial efficiency, which can be applied to antibacterial wound dressings, tissue scaffolds and food packaging and other fields.

Systems and methods for modeling contraction deflection of a flexible tissue scaffold are disclosed herein. The systems and methods include obtaining images of a flexible stent at a plurality of points in time, wherein the flexible stent is depicted to deflect along a first dimension due to contraction forces exerted thereon at the plurality of points in time. Curves are fitted to the images such that each curve extends along a centerline of the flexible stent within the respective image. Displacement values are determined from the curves that provide measurements along the first dimension between respective curves and a reference line extending along a second dimension perpendicular to the first dimension. A model may then be generated based on the displacement values. The model characterizes contraction forces exerted on the flexible stent over the plurality of points in time.

Disclosed herein is a bioactive polymer for forming a tissue scaffold, the polymer comprising a first monomer for binding water, a second monomer for imparting mechanical properties to the scaffold; optionally, a third monomer for binding to a natural or synthetic peptide or protein (NSPP); and a fourth monomer for imparting phase-transition behaviour, wherein the scaffold forms a malleable structure upon hydration. Preferably, the first monomer is OEGMA; the second monomer is PLA / HEMA; the third monomer is NAS; and the fourth monomer is NIPAAm, and the polymer comprises: OEGMA in an amount of from about 1 to about 15 mol %; PLA / HEMA in an amount of from 5 to about 50 mol %; NAS in an amount of from 0 to about 15 mol %; and NIPAAm in an amount of up to about 85 mol %.

Polymeric substrates, optionally in sheet form, treated with a thin layer of photoresist are perforated by laser ablation. Following removal of the photoresist the polymer substrates perforated with holes are patterned in stripes by photolithography, which is followed by synthesis of a cell-adhesive organometallic / self-assembled monolayer of phosphonate (SAMP) interface in the exposed regions, providing well-aligned continuous stripes for various levels of perforation. Cells plated on each of these 2-dimensional (2D) perforated surfaces attach to the interface and spread in alignment with pattern fidelity that is as high as that measured on a non-perforated, patterned substrate. A stack of such 2D patterned polymers yields a 3-dimensional (3D) device which facilitates cell growth and viability via the perforations.

A method and apparatus for degrading a hypodermal tissue scaffold involves exposing the scaffold to a low-intensity focused ultrasonic beam such as to burst gas vesicles trapped in the silk fibroin from which scaffold is constructed. The rate of degradation van be controlled using ultrasonic beams of varying intensities. In one embodiment, the ultrasonic beams are administered trans-dermally to a hypodermal scaffold.

Tissue scaffolds for neural tissue growth have a plurality of microchannels disposed within a sheath. Each microchannel comprises a porous wall having a thickness of ≤about 100 μm that is formed from a biocompatible and biodegradable material comprising a polyester polymer. The polyester polymer may be polycaprolactone, poly(lactic-co-glycolic acid) polymer, and combinations thereof. The tissue scaffolds have high open volume % enabling superior (linear and high fidelity) neural tissue growth, while minimizing inflammation near the site of implantation in vivo. In other aspects, methods of making such tissue scaffolds are provided. Such a method may include mixing a reduced particle size porogen with a polymeric precursor solution. The material is cast onto a template and then can be processed, including assembly in a sheath and removal of the porogen, to form a tissue scaffold having a plurality of porous microchannels.

A method and apparatus for degrading a hypodermal tissue scaffold involves exposing the scaffold to a low-intensity focused ultrasonic beam such as to burst gas vesicles trapped in the silk fibroin from which the scaffold is constructed. The rate of degradation can be controlled using ultrasonic beams of varying intensities. In one embodiment, the ultrasonic beams are administered trans-dermally to a hypodermal scaffold.

The present application belongs to the field of medical preparations, and particularly relates to an ultrasonic imaging hydrogel, a preparation method and application thereof. The present application adds imaging particles to the hydrogel system, so that the system has good ultrasonic imaging effect. Meanwhile, hydroxymethyl cellulose is specifically selected as a suspending agent, which not only significantly improves the stability of the imaging particles in the system, reduces the sedimentation of the imaging particles, but also is beneficial to maintaining the rapid gelation performance of the hydrogel, has great industrialization value and clinical use value, and can provide new materials for the fields of medicine and tissue engineering, and be applied to implantable medical devices, permanent embolization, artificial tissue scaffolds and the like.

The invention discloses a decellularized extracellular matrix modified flexible mesh scaffold for achilles tendon rupture repair and a preparation method thereof, and belongs to the technical field of degradable tissue scaffolds. The stent provided by the invention adopts a double-layer composite structure design, and the outer layer is a PCL / ECM gel mixed electrostatic spinning layer and loads natural growth factors; a degradable PCL hollow support is printed on the inner layer in a fused deposition 3D mode and used for providing mechanical support for a main body. According to the stent, a bioactive layer is constructed, natural slow release of growth factors is achieved, uncontrollability of exogenous addition is avoided, the tendon regeneration induction capacity is remarkably enhanced, the achilles tendon repair capacity is preliminarily verified in a rabbit achilles tendon injury model, an ECM / PCL degradable material is adopted, secondary operation is avoided, and the stent has excellent medical application prospects.