39 results about "Artificial tissue" patented technology

Provided is a hybrid hydrogel composition and methods for its preparation and use in 3D printing biomaterials for tissue engineering applications. The hybrid hydrogel composition comprises discrete hydrogel particles of a first crosslinked polymeric network, interpenetrated- through and threaded by a second crosslinked polymeric network, dispersed in a medium. The first polymer is a thermo-responsive polymer, while the second is a protein-based thermo¬ denaturing polymer. The process for preparing this composition involves mixing swelled hydrogel nanoparticles of the first polymer with a non-crosslinked hydrogel of the second polymer in the presence of a crosslinking agent for the first polymer. The 3D printing method utilizes this composition below the LCST of the thermo-responsive polymer, followed by temperature increase wherein the second polymer cures and fixes the printed ink. This approach enables the creation of high-resolution structures, including artificial tissues with capillary blood vessels, featuring dimensions less than 50 μm.

Methods of differentiating a mesenchymal stromal cell (MSC) to a first non-MSC cell fate, the method comprising contacting the MSC with extracellular vesicles (EVs), matrix-bound vesicles (MBVs) or a combination thereof are provided. Methods of producing artificial tissue by culturing MSCs with two sets of vesicles each comprising EVs, MBVs or both that differentiate MSCs to two different non-MSC cell fates, methods of culturing with reduced growth factors and method of differentiating an MSC to a muscle cell fate are also provided.

The present invention relates to the technical field of biomaterials, and discloses a biodegradable polyurethane elastomer, a preparation method thereof and an application. The preparation method comprises the following steps: (1) Using polycaprolactone diol, poly-3-hydroxybutyrate, polyethylene glycol, diisocyanate and 2-hydroxyethyl methacrylate as raw materials, under a nitrogen or inert gas environment, preparing a vinyl-terminated polyurethane prepolymer; (2) Heating the product in (1), adding a diluent, mixing evenly, adding an initiator, and raising the temperature for reaction for 3 to 4 h to obtain a biodegradable polyurethane elastomer; using the prepared biodegradable polyurethane elastomer to prepare an artificial tissue engineering hard bone tissue material. The present invention uses three kinds of diols as raw materials, as a mixed soft segment for preparing the biodegradable polyurethane elastomer, exerts the synergistic effect of the three kinds of diols, and regulates the biodegradation rate of the biodegradable polyurethane elastomer by controlling the mass ratio and molecular weight of the three.

The invention provides an egg white-sourced protein-based biological hydrogel as well as a preparation method and application thereof. The method comprises the following steps: separating and purifying ovomucoid in egg white by adopting an ethanol fractional precipitation method, and accurately controlling the final concentration of ethanol in a system in two ethanol precipitation processes, so that the final concentration a of ethanol in the system in the primary ethanol precipitation process and the final concentration b of ethanol in the system in the secondary ethanol precipitation process meet the condition that a is greater than or equal to 40 vol% and less than or equal to 62%; other miscellaneous proteins, salts and other small molecular impurities in the egg white can be removed, and the ovomucoid is efficiently separated and purified from the egg white; the modified ovomucoid obtained by covalently modifying the ovomucoid separated and purified by the method by using a modifier has the rheological characteristics of rapid crosslinking, shear thinning and the like, and can form the protein-based biological hydrogel under the action of a photoinitiator. The rapid prototyping and mechanical properties of the hydrogel are met, and the hydrogel has a wide application prospect in preparation of 3D biological printing materials, 4D biological printing materials, artificial organs, artificial tissues and dressings.

Systems and methods for determining spontaneous behavior of engineered tissues are described herein. In one example aspect, a waveform may be obtained that includes a functional response of an engineered tissue over a period of time. A frequency-based global classifier may be applied to the waveform to generate a first classification score, where the first classification score indicates whether the waveform includes periodic constrictions of the engineered tissue over the time period. When the first classification score indicates that periodic contraction does not exist within the waveform, a local classifier may be applied to the waveform, thereby generating a second classification score. The second classification score indicates whether the waveform includes spontaneous constriction of the engineered tissue over the time period. A behavioral feature may then be generated for the engineered tissue during the time period based on the second classification score.

The present invention relates to a process for three-dimensional shaping of a biological and / or artificial tissue / tissue component, which enables a desired three-dimensional, reproducible and permanent shaping of the tissue / tissue component via crosslinking by means of a suitable crosslinking agent in combination with a rigid shaped body, in particular a single rigid shaped body, and a granulate.

The invention relates to a fully disposable system for casting, polymerizing and compressing a hydrogel. The invention further relates to a method for producing a scaffold for the generation of artificial tissue products using said disposable system.

An artificial tissue includes: a first layer that is made of a conductive material and is provided on a front surface side of the artificial tissue, the first layer having a front surface portion and a side surface portion; a second layer that is made of a conductive material and is provided on a back surface side of the first layer, the second layer having a front surface and a side surface; and a support layer that supports the first layer and the second layer from a back surface side of the artificial tissue, in which the front surface portion of the first layer covers the front surface of the second layer, and the side surface portion of the first layer covers the side surface of the second layer.

The present disclosure provides a high-throughput 3D cell spheroid culture chip, the preparation process and uses thereof. Through various efficacy experiments in the present disclosure, first evidence of using hydrogels derived from decellularized liver tissue as a self-healing biomaterial to reduce damage to damaged hepatocytes and enhance liver function in vitro is provided. Integrating endothelial cell-covered hepatocyte spheroids into DLM-CP hydrogels is a promising approach to develop microbial liver tissue, providing a potential solution for liver fibrosis recovery and promoting cell-level therapy. DLM-CP hydrogels show great potential for cell encapsulation for therapeutic purposes in future clinical settings and may be applied to ultra-high-throughput three-dimensional cell spheroid culture chips. It is used to create artificial tissues and organs, becoming a high-value tool widely used in biomedical research and pharmaceutical fields.

Provided is a highly safe articular cartilage regeneration material. This articular cartilage regeneration material contains: a scaffold-free artificial tissue in which synovial-derived mesenchymal stem cells cultured with a serum-free medium form a three-dimensional structure; and an extracellular matrix derived from mesenchymal stem cells. The articular cartilage regeneration material is implanted so as to be attached to a site of lost or damaged articular cartilage.

Systems and methods for processing functional response waveforms obtained from artificial tissue are described herein. In one example aspect, the systems and methods include obtaining a first waveform including a systolic response and a relaxation response of artificial tissue during a systolic-relaxation cycle. A model is then fitted to the first waveform, wherein the model independently parameterizes the growth of the contraction response and the relaxation response. A second waveform is then generated from the model fitted to the first waveform such that the second waveform includes a noise filtered representation of the first waveform. Feature values may then be extracted from the second waveform, which feature values may be used for noise filtering and to achieve a high fidelity and high capacity synthetic training dataset to develop artificial intelligence (AI) or otherwise for downstream tasks, such as drug discovery and development.

Systems and methods for geometric coding of tissue phenotypes are described herein. In at least some aspects, the systems and methods include obtaining images of artificial tissue under a first set of conditions. The system and method further includes extracting a first region from the image such that the first region defines the artificial tissue within the image. The systems and methods further include extracting one or more geometric features from the first region, wherein the one or more geometric features provide a shape-based characterization of the artificial tissue. The systems and methods further include generating a first tissue descriptor based on the one or more geometric features. The first tissue descriptor may encode a phenotype of the artificial tissue under the first set of conditions. Still further, in some aspects, the systems and methods may include implementing quality control (QC) of artificial tissue.

System and methods for modelling contractile deflection of flexible tissue scaffolds are disclosed herein. The system and methods comprise obtaining images of a flexible scaffold at a plurality of time points, where the flexible scaffold are depicted as deflected along a first dimension due to contractile forces exerted thereupon at the plurality of time points. Curves are fit to the images such that each curve extends along a centerline of the flexible scaffold within a respective image. Displacement values are determined from the curves, which provides a measurement along the first dimension between a respective curve and a reference line extending along a second dimension perpendicular to the first dimension. A model may then be generated based on the displacement values. The model characterizes contractile forces exerted on the flexible scaffold over the plurality of time points.

Systems and methods for detecting spontaneous tissue contraction are described herein. In one example aspect, a functional response waveform may be processed that includes obtaining a first waveform that includes at least one contraction response and at least one relaxation response of engineered tissue. A predicted contraction type may be determined for a plurality of contraction types (e.g., a single type and a dual type) of the first waveform. A model may be fitted to the first waveform based on the predicted contraction type, wherein the model parameterizes a growth of at least one contraction response of the engineered tissue independently of a growth of at least one relaxation response of the engineered tissue. A second waveform may be generated from the model fitted to the first waveform such that the second waveform includes a noise filtered representation of the first waveform.

The present inventions is to produce a tissue engineered tube including a smooth muscle layer and an endothelial cell layer derived from stem cells. It is possible to produce a tissue engineered tube that generates physiological contraction and relaxation reactions by producing a tissue engineered tube using smooth muscle cells derived from stem cells at least three times or more as those used in a method of the related art, and using endothelial cells derived from stem cells. Since an artificial blood vessel produced using iPS cells derived from a Werner syndrome patient indicates being an artificial blood vessel reflecting a pathological condition, tissue engineered tubes reflecting pathological conditions of various diseases can be produced using iPS cells. The tissue engineered tube can be used in pharmaceutical screening and transplantation.

Systems and methods are described herein for processing a functional response waveform obtained from an artificial tissue. In one example aspect, the systems and methods include obtaining a first waveform comprising a contraction response and a relaxation response of artificial tissue during a contraction-relaxation cycle. A model is then fitted to the first waveform, where the model independently parameterizes growth of the contraction response and the relaxation response. A second waveform is then generated from the model fit to the first waveform such that the second waveform comprises a noise filtered representation of the first waveform. Feature values(s) can then be extracted from the second waveform, which can be used to perform noise filtering and to enable high-fidelity and high-volume synthetic training data sets to develop artificial intelligence (Al) or otherwise for downstream tasks such as drug discovery and development.

The invention relates to a fully disposable system for casting, polymerizing and compressing a hydrogel. The invention further relates to a method for producing a scaffold for the generation of artificial tissue products using said disposable system.

The invention relates to a method for constructing an anisotropic artificial tissue based on direct writing of cell-loaded micro-nano fibers, and belongs to the technical field of tissue engineering. According to the method, living cells are directly loaded into various types of bioactive materials through a near-field direct writing technology, and micro-nano biological fibers with specific structures and functions are formed by utilizing a visible light crosslinking technology. The fibers can simulate a network structure of a natural extracellular matrix, provide an excellent growth environment for cells and realize artificial tissue construction, so that repair and regeneration of tissues are promoted.

Systems and methods for detecting spontaneous tissue contraction are described herein. In one exemplary embodiment, a functional response waveform may be processed, including obtaining a first waveform that includes at least one contraction response and at least one relaxation response of the manipulated tissue. A predicted contraction type may be determined from among a plurality of contraction types (e.g., single and dual types) for the first waveform. A model may be fitted to the first waveform based on the predicted contraction type, and the model parameterizes the growth of at least one contraction response of the manipulated tissue independently of the growth of at least one relaxation response of the manipulated tissue. A second waveform may be generated from the model fitted to the first waveform such that the second waveform includes a noise-filtered representation of the first waveform.

The invention relates to a preparation method of an intercellular substance-containing artificial tissue, which comprises the following steps: introducing a phenylboronic acid group on a protein vesicle by a method of copolymerization of N-isopropylacrylamide and 3-acrylamido phenylboronic acid; the preparation method comprises the following steps: constructing aggregate micro-droplets by taking polydiene dimethyl ammonium chloride and carboxylated amylose as primitives; based on a biological orthogonal reaction of phenylboronic acid and polysaccharide, polysaccharide aggregate droplets are used as intercellular substances to adhere phenylboronic acid modified protein vesicles together to form an aggregate phase with a microstructure similar to that of a biological tissue. According to the method, the problems that the aggregate microdroplets cannot stably exist in an aqueous solution for a long time and the application of the aggregate microdroplets is limited to the micro-scale are solved, a stable aggregate phase can be formed on the macro-scale, and biological enzyme reaction under low concentration is enriched and activated; optimization thoughts in the aspects of consumption saving, enrichment and recovery, enzyme activity regulation and control and the like are provided for some high-value biological enzymes in the using process.

Systems and methods for modeling contraction deflection of a flexible tissue scaffold are disclosed herein. The systems and methods include obtaining images of a flexible stent at a plurality of points in time, wherein the flexible stent is depicted to deflect along a first dimension due to contraction forces exerted thereon at the plurality of points in time. Curves are fitted to the images such that each curve extends along a centerline of the flexible stent within the respective image. Displacement values are determined from the curves that provide measurements along the first dimension between respective curves and a reference line extending along a second dimension perpendicular to the first dimension. A model may then be generated based on the displacement values. The model characterizes contraction forces exerted on the flexible stent over the plurality of points in time.

The purpose of the present invention is to provide tendon / ligament-like artificial tissues with sufficient strength. The present invention provides tendon / ligament-like artificial tissues having sufficient strength, by embedding mesodermal stem cells, mesenchymal stem cells, or such in a gel having strength to withstand tension loading, and culturing the cells while applying tension loading to the gel and while adding cells and gel. Human iPS cells, tissue stem cells, or such can be used for mesodermal and mesenchymal stem cells, and such.

This application relates to pre-assembled bioprosthetic valves and sealing catheters. The valved catheter includes a bioprosthetic valve, such as a heart valve, and a tubular catheter sealed with a bioresorbable material. The bioprosthetic heart valve comprises artificial tissue that has been treated to allow for long-term dry storage without valvular functional decline. The bioprosthetic heart valve may have independent bovine pericardial leaflets or a whole porcine valve. The sealing catheter includes a tubular matrix impregnated with a bioresorbable medium such as gelatin or collagen. The valved catheter is stored dry in an encapsulation material, wherein a desiccant bag is provided with a limited capacity to absorb moisture from the encapsulation material to prevent the bioprosthetic tissue from drying beyond a point that would impair its usability as a bioprosthetic heart valve. The heart valve may be sutured within the sealing catheter or coupled to the sealing catheter via a snap-fit ​​connection.

The invention discloses a method for preparing high-strength regenerated cellulose filaments, and belongs to the technical field of textiles. The preparation method of the high-strength regenerated cellulose filament comprises the following steps: firstly, pretreating bacterial cellulose to obtain a bacterial cellulose filter cake; dissolving the bacterial cellulose filter cake in NMMO (N-methylmorpholine-N-oxide) to obtain a transparent bacterial cellulose solution; adding a cross-linking solution prepared from tannic acid and / or polyethylene glycol diglycidyl ether into the bacterial cellulose solution for cross-linking reaction to obtain a spinning solution; and finally, carrying out wet spinning on the spinning solution to obtain the high-strength regenerated cellulose filament. The high-strength regenerated cellulose filament has high mechanical performance and stability, and can be widely applied to artificial tissue repair materials and drug sustained-release carriers in the field of biomedicine, antibacterial packaging materials in the field of food packaging and the like.

The invention relates to a fully disposable system for casting, polymerizing and compressing a hydrogel. The invention further relates to a method for producing a scaffold for the generation of artificial tissue products using said disposable system.

The present invention relates to a phenol derivative-modified, tissue-derived extracellular matrix derivative for construction of artificial tissues. A hydrogel composition of the present invention and a hydrogel prepared therefrom exhibit various effects such as hemostasis, blood coagulation acceleration, cell transplantation, drug delivery, cell differentiation promotion, etc. and thus can be applied to single or composite uses. Furthermore, the present invention is excellent in biocompatibility due to its biodegradability and being almost free of cytotoxicity, thus exhibiting very high applicability. In addition, a tissue structure including a single type or multiple types of cells can be formed through the cell-proliferating potential and adhesion of the hydrogel of the present invention and can better simulate in-vivo microenvironments.

A system and method for determining the spontaneous behavior of manipulated tissue are described herein. In one exemplary embodiment, a waveform containing the functional response of manipulated tissue over a period of time may be obtained. A frequency-based global classifier may be applied to the waveform to generate a first classification score, which indicates whether the waveform contains periodic contractions of the manipulated tissue over the period. If the first classification score indicates that periodic contractions are not present in the waveform, a local classifier may be applied to the waveform to generate a second classification score, which indicates whether the waveform contains spontaneous contractions of the manipulated tissue over the period. Then, based on the second classification score, a behavioral profile of the manipulated tissue over the period can be generated.

The present invention discloses a method for evaluating the efficacy of a pharmaceutical agent suspected to be effective in treating a disease, comprising the steps of: (i) incubating an artificial tissue with the pharmaceutical agent, wherein the artificial tissue is or has been modified to express the disease; (ii) determining at least one physical parameter of the artificial tissue obtained in step (i), wherein the physical parameter is an indicator of the functionality of a physiological bodily function; and (iii) determining whether the value of the at least one physical parameter determined in step (ii) satisfies a predetermined threshold value for the physical parameter, thereby determining whether the pharmaceutical agent is effective; and if the value of the at least one physical parameter exceeds the predetermined threshold value, it is indicated that the pharmaceutical agent is effective in treating the disease by restoring at least a portion of the functionality of the physiological bodily function.

The present application belongs to the field of medical preparations, and particularly relates to an ultrasonic imaging hydrogel, a preparation method and application thereof. The present application adds imaging particles to the hydrogel system, so that the system has good ultrasonic imaging effect. Meanwhile, hydroxymethyl cellulose is specifically selected as a suspending agent, which not only significantly improves the stability of the imaging particles in the system, reduces the sedimentation of the imaging particles, but also is beneficial to maintaining the rapid gelation performance of the hydrogel, has great industrialization value and clinical use value, and can provide new materials for the fields of medicine and tissue engineering, and be applied to implantable medical devices, permanent embolization, artificial tissue scaffolds and the like.

The present invention discloses a detection method and device for artificial tissues and organoids. The present invention uses a deep learning network for artificial tissue and organoid segmentation to achieve the segmentation of artificial tissues and organoids, and obtains three-dimensional images of artificial tissue and organoid regions; its labels are images after contrast enhancement; morphological indexes and ATP values of artificial tissues and organoids in each culture well of the corresponding culture plate on the last day of culture are calculated according to connected components; a covariance matrix is calculated for the matrix constructed after standardizing the morphological indexes and ATP values of artificial tissues and organoids; the covariance matrix is subjected to singular value decomposition to obtain a right singular matrix and a singular value matrix; principal components are extracted according to the variance contribution rate to obtain comprehensive indexes of artificial tissues and organoids. The comprehensive indexes of artificial tissues and organoids are used for the study of drug efficacy.