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Bionic vascularized soft tissue with multilayer vascular structure and preparation method thereof

A bionic blood vessel and vascular structure technology, applied in the field of bionic vascularized soft tissue and its preparation, can solve the problems of low efficiency and complex biological factor regulation

Active Publication Date: 2018-02-16
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, some studies have constructed mature vascular structures by attracting and recruiting smooth muscle cells and fibroblasts from the parenchymal area around the channel to the endothelial layer of the channel. This method is inefficient and requires complex biological factor regulation

Method used

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  • Bionic vascularized soft tissue with multilayer vascular structure and preparation method thereof

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preparation example Construction

[0046] figure 1 A schematic diagram of the preparation method for constructing a biomimetic vascularized soft tissue with a multi-layered vascular structure in a hydrogel provided by the present invention, including the following steps:

[0047] 1) Obtain a sacrificial mold with a branched structure by casting or 3D printing; as figure 1 Step 1 in;

[0048] 2) Coating or spraying the polymer solution mixed with a porogen on the surface of the above-mentioned branched structure, solidifying to form a film, such as figure 1 Step 2 in;

[0049] 3) Place the structure obtained in step 2) in an aqueous solution, dissolve the sacrificial mold and porogen, and obtain a vessel-like channel structure with a certain wall thickness and surface pores, such as figure 1 Step 3 in;

[0050] 4) Plant endothelial cells in the channel to simulate the endothelial layer of the vascular structure; plant cells such as smooth muscle cells or fibroblasts in the surface pores of the channel struct...

Embodiment 1

[0057] Example 1. Preparation of biomimetic vascularized soft tissue with multi-layered vascular structure

[0058] Polylactic acid (PLA, molecular weight 50,000, viscosity 1.5 dL / g) was dissolved in chloroform (CHCl 3 ), prepare a polylactic acid (PLA) solution with a concentration of 50 mg / mL, and add sodium chloride (NaCl) particles (with a diameter of 75±25 μm), wherein sodium chloride (NaCl):polylactic acid (PLA)=8:2 (wt%), uniformly stirred to obtain a polylactic acid (PLA) solution containing sodium chloride (NaCl).

[0059] Add the gelatin (Gelatin, 15k~25kDa, 0.15g / ml) solution into the syringe nozzle of the extrusion 3D printing device at 37°C, generate a printing path according to the preset structure, and print at 4°C to form a two-level branch Morphological structure (500-1500 μm in diameter). The surface of the printed structure was uniformly coated with a polylactic acid (PLA) solution containing sodium chloride (NaCl), and air-dried for 12 hours to obtain a p...

Embodiment 2

[0062] Example 2. Preparation of bionic vascularized soft tissue with multi-layered vascular structure

[0063] Dissolve polylactic acid-glycolic acid copolymer (PLGA, the molar ratio of polylactic acid (PLA) to polyglycolic acid (PGA) is 75:25, viscosity is 0.93dl / g) in 1,4 dioxane (1, 4-dioxane), prepare a solution of polylactic acid-glycolic acid copolymer with a concentration of 20 mg / mL, and add sodium chloride (NaCl) particles (with a diameter of 100±25-μm), wherein sodium chloride (NaCl): polylactic acid Glycolic acid copolymer (PLGA)=7:3 (wt%), uniformly stirred to obtain a polylactic acid-glycolic acid copolymer (PLGA) solution containing sodium chloride (NaCl).

[0064] Mix sucrose, maltose, and glucose uniformly at a mass ratio of 1:12:2, put them into the nozzle cavity of the melt extrusion printing equipment, and heat and melt at 130°C, and control the continuous extrusion of the nozzle by air pressure. Set the structure to generate a printing path, and form a st...

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Abstract

The invention discloses a bionic vascularized soft tissue with a multilayer vascular structure and a preparation method thereof. The preparation method comprises the steps of: employing a moulding process or 3D printing way to obtain a sacrificial membrane with a branched structure; coating or spraying a polymer solution containing a pore-foaming agent on the surface of the sacrificial membrane toobtain a polymer film; removing the treated sacrificial membrane of the structure and the pore-foaming agent to obtain a vascular channel-like structure with surface pores; conducting perfusion planting of endothelial cells in the channel of the vascular channel-like structure; planting smooth muscle cells or fibroblasts in the surface pores of the vascular channel-like structure; and placing thetreated vascular channel-like structure in a mold, adding an aquogel solution containing parenchymal cells, and conducting moulding so as to obtain the bionic vascularized soft tissue. The method provided by the invention can construct bionic blood vessels with a multilayer structure in artificial tissues.

Description

technical field [0001] The invention relates to a bionic vascularized soft tissue with a multi-layered blood vessel structure and a preparation method thereof, which belong to the technical field of tissue engineering and biomanufacturing. Background technique [0002] Tissue Engineering is a cutting-edge interdisciplinary science. Its basic principle is to repair and replace damaged tissues by constructing bionic artificial tissues with certain structures and functions in vitro. Although tissue engineering has been partially applied in the fields of skin, bone / cartilage, bladder, and blood vessels, it still faces great challenges in the construction of complex and bulky tissues, among which vascularization is one of the biggest challenges in tissue engineering. For tissues with vigorous metabolism, such as myocardium and liver, due to the high level of material and energy metabolism of cells, these tissues have complex and rich vascular networks inside. In tissue engineeri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/38A61L27/52B33Y10/00B33Y70/00G09B23/28
Inventor 张婷方永聪孙伟贾波
Owner TSINGHUA UNIV
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