39 results about "Vascular channel" patented technology

The invention provides a micro-fluidic chip, a model for realizing accurate positioning of an organoid and sequential interaction of a tumor microenvironment, and a construction method of the model. The micro-fluidic chip comprises a chip main body and a chip bottom plate, a blood vessel channel, a central channel, a neural immune cell channel and a culture medium channel are sequentially arranged on the lower surface of the chip main body; the chip bottom plate is arranged on the lower surface of the chip main body and seals each channel; one side of the blood vessel channel is communicated with one side of the central channel, the other side of the central channel is communicated with one side of the neural immune cell channel, and the other side of the neural immune cell channel is communicated with one side of the culture medium channel; the upper surface of the chip main body is provided with an open hole, and the open hole penetrates through the chip main body and is communicated with the middle position of the central channel. According to the model manufactured by the micro-fluidic chip, accurate positioning of tumor organs can be realized through combination of an automatic positioning device and physical constraint, and multi-dimensional cell interaction is supported by dynamic environment regulation and control.

The invention discloses a bone organ chip loaded with a microelectrode array and a use method, and relates to the technical field of organ chips, the bone organ chip is made of a polydimethylsiloxane material, and the bone organ chip comprises a three-layer structure: a top layer, a middle layer and a bottom layer; the middle layer comprises two blood vessel micro-channels, two osteoblast micro-channels and a neuron micro-channel, the channels are separated through columns and micro-columns, and cell interaction in a bone micro-environment is simulated; a microelectrode array is arranged on the top layer and is used for monitoring electrical activity of neurons in real time; the bottom layer is provided with a pneumatic valve which can control opening and closing of the micro-channel. According to the invention, nerve and blood vessel channels are introduced, a nerve-blood vessel-immunoregulation bone microenvironment is constructed, physiological processes such as osteogenesis-blood vessel formation coupling and the like are embodied, intervals of different sizes can enhance intercellular crosstalk and allow blood vessels and neuron axons to selectively pass through, so that the chip can simulate a complex microenvironment of bone tissues, and the microenvironment of the bone tissues can be simulated; and an efficient and accurate in-vitro model is provided for bone disease mechanism research and drug development.

The invention relates to a tissue engineering trachea, which combines an additive manufacturing technology with a biological material, simulates the form and the function of a natural gas pipe, designs a cartilage-fiber alternating ring structure and a bionic fiber band structure, and designs a unique blood supply channel in a fiber section. The trachea fiber section is composed of hydrogel with a hierarchical porous structure, and a circumferential blood vessel channel and a radial blood vessel pore channel are designed in the hydrogel so as to promote ingrowth and growth of new blood vessels and optimize the blood supply reconstruction process. The tracheal cartilage section is made into a C-shaped cartilage ring with cartilage cells through a biological printing technology, and necessary mechanical support is provided. The fiber segment and the cartilage segment are effectively integrated through chemical bonds of functional groups to ensure stable connection of the two. Partitioned construction of fibers and cartilage is achieved through the biological 3D printing technology, the bionic tissue engineering trachea is formed in a modular construction-integrated assembly mode, it is verified that repairing and reconstruction of multiple types of tissue bionic tracheas can be achieved, and an innovative solution is provided for tracheal injuries.

The invention belongs to the technical field of organ chips, provides an in-vitro brain lymphatic system chip construction method and a brain lymphatic system chip, and makes up the blank of brain lymphatic system in-vitro model construction. The method comprises the following steps: firstly, carrying out flushing treatment, disinfection and sterilization treatment, rinsing treatment and cell culture on a micro-fluidic channel in the chip; then, in the treated micro-fluidic channel, blood vessel channel cell inoculation and brain parenchyma channel cell inoculation are carried out; and finally, in the blood vessel channel, perfusion is carried out once at a preset flow speed every preset duration, shear force stimulation simulating pulse type blood flow is provided, basic formation of in-vitro cerebral vessels is achieved after continuous perfusion, perfusion is carried out once at a preset flow speed every preset duration, shear force stimulation simulating pulse type blood flow is provided, and in-vitro cerebral vessels are formed. Intermittent liquid flow simulating arterial fluctuation is achieved.

The indwelling needle suite comprises a catheter assembly, a puncture assembly and a negative pressure assembly, and the catheter assembly comprises a catheter base, a catheter connected with the catheter base and an isolation plug arranged in the catheter base; the puncture assembly comprises a needle seat and a needle tube connected with the needle seat, the needle seat is provided with a first end and a second end, the first end of the needle seat is detachably connected with the catheter seat, and a needle tip of the needle tube penetrates through the first end of the needle seat and the isolation plug and penetrates out of the catheter; the negative pressure assembly comprises a negative pressure generator and a channel connection switch, the negative pressure generator is provided with a volume-variable inner cavity, and the channel connection switch is arranged between the negative pressure generator and the needle seat and can be used for communicating or blocking the inner cavity of the needle seat and the inner cavity of the negative pressure generator. The indwelling needle suite can conveniently and rapidly build puncture negative pressure and observe blood return, can automatically reduce the negative pressure to prevent injury after blood return, and is especially suitable for first aid and rapid building of a blood vessel channel for administration when the blood vessel condition is poor.

The present disclosure relates to an interventional device for implanting an electrode into the brain through a blood vessel, the blood vessel having a blood vessel channel and a blood vessel wall, characterized in that the interventional device comprises: a first guide, the distal end of which is movable distally within the blood vessel channel and establishes a first support point within the blood vessel channel; a second guide, the distal end of the second guide being movable distally through the first guide in the vascular channel and establishing a second support point in the vascular channel such that the second guide extends in an arcuate manner between the first support point and the second support point; the puncture suite is used for carrying an electrode, and the far end of the puncture suite can penetrate through the first guiding piece to move towards the far side in the blood vessel channel and pierce the blood vessel wall in a target puncture area between the first supporting point and the second supporting point, so that the carried electrode is implanted into the brain. Furthermore, the present disclosure relates to a medical device.

The invention discloses a three-channel bionic bone organ chip and a method thereof.The three-channel bionic bone organ chip comprises a three-channel model and a carrier, the three-channel model comprises a blood vessel channel in the middle and bone channels located on the two sides of the blood vessel channel, the width of the blood vessel channel is 500 micrometers, the width of the bone channels is 1000 micrometers, and the width of the carrier is 100 micrometers. The two sides of the blood vessel channel are separated from the bone channels on the two sides through long barriers respectively. The carrier is used for bearing the three-channel model. According to the invention, a novel bone organ chip (Bone Orga-on-a-Chip, BOC) is constructed, which is convenient for simulating an OP microenvironment induced by glucocorticoid and the influence of mechanical mechanics on treatment of OP by MSCs. On the basis, a candidate gene TPD52L1 is further analyzed and screened through bioinformatics, the candidate gene TPD52L1 is remarkably up-regulated in MSCs induced osteogenic differentiation, and the effect of the gene in MSCs osteogenic adipogenic fate choice is discussed.

This invention relates to the field of microfluidic chip technology, specifically to a dynamic microfluidic chip device, its preparation method, and its applications for studying CAR-T therapy for gliomas. The preparation method includes: fabrication of the microfluidic chip; reconstruction of neural tissue; reconstruction of the blood-brain barrier; and simulation of the glioma microenvironment. The dynamic microfluidic chip device includes a microfluidic chip; the microfluidic chip includes, from left to right, a vascular channel, a brain parenchyma channel, a tumor channel, and a subarachnoid channel; each of the vascular channel, brain parenchyma channel, tumor channel, and subarachnoid channel includes a cell culture chamber with an inlet on one side; the cell culture chambers of the vascular channel and the subarachnoid channel have corresponding outlets on the other side. The applications include: CAR-T cell infusion; observation of transmembrane migration; and detection of biomarkers. This invention, through the dynamic microfluidic chip device, its preparation method, and its applications, aims to achieve more accurate in vitro evaluation of CAR-T cell therapy.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

The present disclosure relates to an interventional device for implanting an electrode into a brain through a blood vessel having a blood vessel passage and a blood vessel wall, characterized in that the interventional device comprises: a first guide, a distal end of which is movable distally within the blood vessel passage and establishes a first support point in the blood vessel passage; a second guide, a distal end of which is movable distally within the blood vessel passage through the first guide and establishes a second support point in the blood vessel passage, such that the second guide extends arcuately between the first support point and the second support point; and a puncture set for carrying the electrode, and a distal end of which is movable distally within the blood vessel passage through the first guide and punctures the blood vessel wall in a target puncture region between the first support point and the second support point, thereby implanting the carried electrode into the brain. Furthermore, the present disclosure relates to a medical device.

An ECM-embedded vascular channel-on-a-chip includes an outer case having an internal chamber, an ECM provided within the chamber, the ECM having a cross-sectionally rounded vascular channel having a first end and a second end opposite the first end, wherein an immer surface of the channel is lined with barrier-forming endothelial cells, and an inlet conduit coupled to the first end of the channel through a first side of the outer casc and an outlet conduit coupled to the second end of the channel through a second side of the outer case. Also, an airway-on-a-chip includes an airway lumen including a porous membrane, wherein mucociliated airway epithelial cells are provided on the porous membrane. The airway-on-a-chip has an ECM with embedded stromal cells. The ECM has a cross-sectionally rounded vascular channel, and the inner surface of the channel is lined with barrier-forming endothelial cells.

The present disclosure relates to an interventional device for implanting an electrode into the brain via a blood vessel, the blood vessel having a vascular channel and a vascular wall. The interventional device comprises: a first guide, the distal end of which is capable of distally moving within the vascular channel and establishing a first support point in the vascular channel; a second guide, the distal end of which is capable of passing through the first guide, distally moving within the vascular channel, and establishing a second support point in the vascular channel, such that the second guide extends in an arcuate manner between the first and second support points; and a puncture kit for carrying an electrode, the distal end of which is capable of passing through the first guide, distally moving within the vascular channel, and puncturing the vascular wall in a target puncture area between the first and second support points, thereby implanting the carried electrode into the brain. The present disclosure also relates to a medical device.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

This invention belongs to the field of organ-on-a-chip technology, proposing a method for constructing an in vitro brain lymphatic system chip and a brain lymphatic system chip, filling the gap in the construction of in vitro models of brain lymphatic systems. First, the microfluidic channels in the chip are rinsed, sterilized, washed, and cultured with cells. Then, vascular channel cells and brain parenchyma channel cells are seeded in the treated microfluidic channels. Finally, in the vascular channels, perfusion is performed at a preset flow rate and at preset intervals to provide shear force stimulation mimicking pulsed blood flow. Continuous perfusion achieves the basic formation of in vitro brain blood vessels. The intermittent fluid flow mimicking arterial pulsations is achieved through this preset flow rate and preset interval perfusion.

The application discloses a balloon blocking microcatheter, which comprises a catheter seat, an inner tube, an outer tube, a balloon tube, a developing ring, a soft tip and a stress release tube, and the inner tube has a three-layer structure, namely an inner layer, an intermediate layer and an outer layer. The intermediate layer is a unique hypotube structure, so that the balloon blocking microcatheter has excellent passability in the blood vessel, and the inner cavity is not compressed, so that the subsequent treatment instrument or medicine can enter the body normally and smoothly, thereby improving the treatment effect and safety of the instrument as a whole. The catheter seat has two independent channels, which can avoid the possibility of intercommunication of the two cavities under high injection pressure, thereby improving the use success rate of the instrument. The balloon is made of silicone rubber material and has super high compliance, which can fit and block complex blood vessel channels, thereby improving the overall treatment effect.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

Intravascular catheter devices for treating a zone of attention located within a vascular passageway, along with related systems and methods, are disclosed. A first and second expandable portion are axially aligned with a longitudinal axis of a tubular member and are connected to the same. Each of the first and second expandable portion includes struts moveable between a first position and a second position where the struts are moved outward from the first position and at least one incising member provided at, and extending from, a surface of a respective one of the struts. The second expandable portion is positioned distal to the first expandable portion. The struts of the second expandable portion are radially offset from the struts of the first expandable portion.

Disclosed are apparatus and methods for blood and other biological fluid purification using a membrane with cell containing vascular channel systems and filtration channel systems. Also disclosed are methods of making the apparatus as well as methods of making membranes.

The invention discloses an in-vitro measurement system and method for determining a balloon catheter correction coefficient. The system comprises a constant temperature module and a correction measurement module, the constant temperature module internally comprises a closed loop formed by sequentially connecting a liquid storage tank, a power pump and a test module through a pipeline, and the closed loop is provided with a flow sensor and a pressure sensor; the power pump is in communication connection with the correction measurement module, so that the correction measurement module can control the rotating speed of the power pump; the balloon catheter is arranged in the test module, the test module is used for simulating a blood vessel channel in a human body, and an in-vitro thermistor extension line, a near-end extension tube and a far-end extension tube of the balloon catheter are respectively connected to the correction measurement module through signal lines; a heating device and a temperature sensor are arranged in the constant temperature module, and the temperature sensor is connected with the correction measurement module. According to the invention, accurate correction of pressure and cardiac displacement measurement results of the balloon catheter can be realized, the structure and operation are simple, and the clinical measurement accuracy can be improved.

The invention provides an intervention consumable control method for a vascular intervention surgical robot, and relates to the technical field of medical instruments. The method comprises the steps that when it is detected that a first intervention consumable generates withdrawing motion, image information of a second intervention consumable in a blood vessel channel is obtained; wherein the first intervention consumable is arranged in a tube cavity of the second intervention consumable in a penetrating manner; and determining position information of the second intervention consumable based on the image information, and controlling the second intervention consumable to move according to the position information, so that the second intervention consumable stays at the target blood vessel position. By monitoring the real-time image of the interventional consumable in the blood vessel, the position of the consumable can be accurately determined, and precise control can be automatically performed, so that precise delivery of the interventional consumable in the blood vessel is realized.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

The application relates to the technical field of medical devices, and particularly provides a blood vessel covered stent, which comprises an expansion stent and an artificial blood vessel cover. The expansion stent is inserted into a branch blood vessel through a branch opening of a blood vessel. The expansion stent is provided with an entering part, a main body part and an outflow part from the branch opening to the deep part of the branch blood vessel. The artificial blood vessel cover is attached to the expansion stent. The blood vessel is expanded by the expansion stent, the blood vessel channel is prevented from being narrowed, the normal blood circulation in the blood vessel is maintained, the expansion stent and the inner wall of the blood vessel are separated by the artificial blood vessel cover, the inner wall of the blood vessel is prevented from proliferating into the inner cavity of the expansion stent, and the blood channel in the expansion stent is maintained unobstructed.

Disclosed are an eye phantom assembly, a method for manufacturing the same, and an eye phantom including the same. The eye phantom assembly includes: a nerve fiber layer (NFL)-mimicking layer having superficial vascular channels formed to mimic a nerve fiber layer; a multilayer membrane-mimicking layer bonded to one surface of the NFL-mimicking layer and having a structure in which multiple layers with different scattering coefficients are stacked; an outer plexiform layer (OPL)-mimicking layer bonded to one surface of the multilayer membrane-mimicking layer and having deep vascular channels formed to mimic an outer plexiform layer; an outer membrane-mimicking layer bonded to one surface of the OPL-mimicking layer and formed to mimic the outer cell layer structure of the retina; and a choroid membrane-mimicking layer bonded to one surface of the outer membrane-mimicking layer and having choroidal vascular channels formed to mimic the choroid.

Disclosed are apparatus and methods for blood and other biological fluid purification using a membrane with cell containing vascular channel systems and filtration channel systems. Also disclosed are methods of making the apparatus as well as methods of making membranes.

The application relates to a blood vessel size measurement method, device and computer equipment. The method comprises the following steps: extending an imaging catheter of an intravascular imaging device into a plurality of simulated blood vessels of a blood vessel simulation device, and obtaining a plurality of simulated blood vessel channel images; determining a region of interest corresponding to a measurement parameter of the simulated blood vessel in the simulated blood vessel channel image as a simulated pixel point quantity carrier, and counting the quantity of pixel points contained in the simulated pixel point quantity carrier; constructing a target mapping function between the quantity of pixel points contained in the simulated pixel point quantity carrier and the measurement parameter of the corresponding simulated blood vessel; and determining the measurement parameter of a real blood vessel corresponding to a real blood vessel channel image based on the target mapping function and the actual quantity of pixel points contained in a real pixel point quantity carrier in the real blood vessel channel image. The method can calibrate the quantity of pixel points contained in the real pixel point quantity carrier, and thus obtain an intravascular image effectively alleviating geometric distortion.

The invention relates to the technical field of brain glioma organoid construction, in particular to a method for constructing a brain glioma organoid, which comprises the following steps: step S1, mixing a brain glioma sample and primary brain microvascular endothelial cells according to a cell number ratio of 1: 5, inoculating into a porous structure of a stent after mixing, and singly inoculating the primary brain microvascular endothelial cells into a hollow vascular channel of the stent; s2, the stent in the step S1 is placed in a gas-liquid interface culture device to culture a brain glioma sample, the gas-liquid interface culture device comprises a culture medium, the bottom of the stent is immersed in the culture medium, the top of the stent is exposed to a mixed gas environment, and the brain glioma sample is cultured through the design of the composite stent containing the porous proliferation area and the hollow blood vessel channel. The problems of low medicine permeation efficiency and limited tumor-blood vessel interaction research caused by blood vessel deficiency of a traditional model are solved.

The application belongs to the technical field of brain glioma organoids, and particularly relates to a method for constructing brain glioma organoids, which comprises the following steps: S1, mixing a brain glioma sample with primary brain microvascular endothelial cells at a cell quantity ratio of 1:5, and then inoculating the mixture into a porous structure of a scaffold, wherein the hollow vascular channel of the scaffold is inoculated with the primary brain microvascular endothelial cells; and S2, culturing the scaffold in step S1 in a gas-liquid interface culture device to culture the brain glioma sample, wherein the gas-liquid interface culture device comprises a culture medium, the bottom of the scaffold is immersed in the culture medium, and the top of the scaffold is exposed to a mixed gas environment; through the composite scaffold design comprising the porous proliferation area and the hollow vascular channel, the problems of low drug penetration efficiency and limited tumor-vascular interaction research caused by the vascular deficiency of a traditional model are solved.

The present invention discloses a prestressed vascular chip, which relates to the field of medical vascular technology. The device includes a main body, a first chamber and a third chamber are opened on the surface of the main body, the first chamber is connected to the third chamber through a first channel, and the main body is provided with at least two fourth chambers, the fourth chamber is arranged on one side of the first chamber, and there is a connecting channel between the fourth chambers, and at least two first channels connected to the first chamber are provided on the connecting channel. The vascular chip that can be constructed by the present invention can achieve a mechanical performance morphology similar to the mechanical properties of blood vessels in the body, so as to solve the problem that the mechanical performance state of the vascular channel inside the chip of the prior art is quite different from the actual vascular state of the human body.

The present application provides an intervertebral fusion cage, which comprises a plurality of support bodies and a plurality of connecting bodies for connecting the support bodies, the support bodies are arranged at intervals, the connecting bodies connect adjacent support bodies, the support bodies extend along a load bearing direction, and the outer surfaces of the support bodies are inwardly recessed to form grooves extending along the load bearing direction, each of the support bodies is internally provided with a vascular channel network, the vascular channel network comprises a main channel and a plurality of branch channels, the two ends of the main channel respectively penetrate the upper surface and the lower surface of the support body along the load bearing direction, one end of the branch channel communicates with the main channel, and the other end extends to the groove. The intervertebral fusion cage of the present application can guide the directional growth of osteocytes along the load bearing direction, and realize immediate blood perfusion and global nutrition delivery after implantation, thereby solving the problems of insufficient deep nutrition, disordered bone ingrowth and low fusion efficiency of traditional fusion cages.

Self-expanding plug embodiments may be used in conjunction with vascular closure device embodiments to promote hemostasis at surgical sites or any other suitable location. In some cases, vascular closure device embodiments may include self-expanding plug embodiments in order to promote hemostasis at a surgical site during a vascular closure procedure.