79 results about "Organ Model" patented technology

The invention relates to the technical field of tumor biomedical engineering and organ chips, in particular to a vascularized gastric cancer organ chip and a preparation method thereof.The chip is composed of an integrated micro-fluidic main body, an annular micro-column array and an optical sealing film, and a central culture cavity is divided into a tumor area and a blood vessel area by micro-columns; the preparation method comprises the following steps: injecting a fibrous protein solution containing human umbilical vein endothelial cells and cancer-related fibroblasts into a vascular region, and adding thrombin for in-situ gelation to form a pre-vascularized network; the method comprises the following steps: mixing a patient-derived gastric cancer organ with a methacrylic acid esterified gelatin pre-polymerized solution, injecting the mixture into a tumor area, and carrying out photo-crosslinking immobilization; culturing for 7-14 days under the dynamic perfusion condition of a mixed culture medium to obtain a three-dimensional gastric cancer organ model containing a capillary network; the method is expected to be used for rapid, low-cost and high-throughput screening of chemotherapy or anti-angiogenesis drugs and accurate prediction of individual curative effects of patients.

The invention relates to a patient-specific multi-material bionic tumor organ model and a preparation method thereof. The method comprises the following steps: carrying out separation and three-dimensional modeling on different anatomical structures in an organ of a patient based on medical image data through an image segmentation technology. And establishing a corresponding relationship between the medical image data and different anatomical structures in the organ of the patient based on a three-dimensional modeling result, and restoring three-dimensional information of the tumor. And performing biomechanical measurement on each biological tissue in the general specimen corresponding to the organ of the patient to obtain a biomechanical measurement result of each biological tissue. And performing structure layering on a three-dimensional modeling result based on a biomechanical measurement result, and mapping a plurality of layered tissue areas with a plurality of printing materials matched with biomechanical properties. And preparing an initial bionic model based on the selected printing material and the biomechanical measurement result corresponding to each biological tissue by adopting a 3D printing technology, and post-processing and assembling the initial bionic model to obtain the bionic tumor organ model.

The invention provides a method for evaluating drug enterotoxicity in vitro through an intestinal organ model, and relates to the field of biological medicine. Comprising the following steps: preparing six different culture media, culturing the hESC by using the different culture media, inducing the hESC to respectively perform directional endoderm cell differentiation, posterior intestinal bulb differentiation, intestinal organ differentiation and intestinal organ maturation, and evaluating the drug enterotoxicity by using a prepared intestinal organ model. The intestinal organ model prepared by the invention is similar to human intestinal cells in composition, can accurately reflect the toxic effect of the medicine in the physiological environment in the human body, can be repeatedly obtained in quantity, and avoids clinical risks and ethical constraints; the sensitivity of drugs with different enterotoxicity can be reflected through cell viability, and the method is suitable for rapid evaluation of in-vitro high-flux drug enterotoxicity.

The invention relates to the field of cell culture, in particular to a liver organ model culture device and method.The liver organ model culture device comprises a temperature control box and a box cover arranged on the temperature control box, a pressing column is slidably installed in the middle of the box cover in a penetrating mode, a column spring is wound around the outer side of the pressing column, and one end of the column spring is connected with the upper end of the pressing column; the other end of the column spring is fixed to the upper end of the box cover, a culture piece is arranged at the position, located on the inner side of the box cover, of the upper end of the temperature control box, a plurality of liquid changing holes are formed in the upper end of the box cover in a penetrating mode, a bearing frame is placed at the upper end of the box cover, and a plurality of liquid injection pipes are embedded in the bearing frame in a penetrating mode; a plurality of liquid suction pipes are embedded in the bearing frame in a penetrating mode, and the liquid injection pipes are located on the sides of the liquid suction pipes. According to the invention, the culture effect is improved, the liquid changing efficiency is also improved, and meanwhile, the gel matrix block is prevented from being damaged during liquid changing.

The invention discloses a three-dimensional ultrasonic CT image reconstruction method based on a neural network agent model. The method comprises the following steps: constructing an anatomical real organ model, simulating a wave field by virtue of a numerical solver, and generating a multi-organ training data set; constructing and training a strong scattering neural operator (S2NO) based on an iterative structure of convergence Born series, so as to solve a partial differential equation with a large computational domain and high oscillation; collecting time domain wave field observation data of a to-be-detected part of a patient, and converting the time domain wave field observation data into frequency domain wave field observation data in a set frequency range through Fourier transform; using the trained S2NO as an agent model, executing a full waveform inversion algorithm, and iteratively reconstructing an ultrasonic CT image; according to the method, a three-dimensional ultrasonic CT image of an in-vivo clinical sample is reconstructed, the speed reaches about 10 times that of a traditional ultrasonic CT full-waveform inversion reconstruction algorithm, and the imaging quality similar to the standard magnetic resonance imaging (MRI) resolution is achieved.

Embodiments of the present application relate to a surgical early warning method and device based on ultrasound image visualization, equipment and medium. The method comprises: extracting the corresponding organ surface from the preset voxel model according to the label corresponding to a plurality of preset organs; wherein the organ surface is composed of triangular facets; according to the set frequency, the ultrasound section is refreshed and drawn according to the pose of the ultrasound section; according to the positional relationship between the triangular facets constituting the organ surface and the pose of the ultrasound section, an organ model is drawn; in response to the user's simulated puncture operation on the organ model, the puncture needle is displayed according to the spatial position of the puncture needle. The technical scheme of the embodiments of the present application can realize the effect of improving the safety and accuracy of the operation.

The application discloses a gene editing system for constructing a muscle weakness disease model pig nuclear transfer donor cell with a DOK7 gene mutation and application thereof. The application provides application of DOK7-gRNA2 shown in SEQ ID NO: 16, DOK7-gRNA4 shown in SEQ ID NO: 17 and NCN protein in preparation of a kit. The application further provides a method for preparing a recombinant cell, comprising the following steps: co-transfecting a pig cell with DOK7-gRNA2, DOK7-gRNA4 and NCN protein to obtain a recombinant cell. The recombinant cell is a recombinant cell with a mutated DOK7 gene. The use of any of the above kits is to prepare a recombinant cell, to prepare a muscle weakness model pig, to prepare a muscle weakness cell model, a muscle weakness tissue model or a muscle weakness organ model. The application has great application value for research and development of muscle weakness disease drugs and revealing the pathogenesis of the disease.

The utility model provides a human body model-based low-frequency magnetic field test system, which comprises a human body model, a plurality of organ models and a data acquisition instrument are arranged in the human body model, each organ model is provided with a plurality of magnetic field probes, and the data acquisition instrument is arranged below the organ models and connected with the magnetic field probes. The human body model is filled with an electrolyte solution, and the organ model and the data acquisition instrument are both placed in the electrolyte solution. The magnetic field probe solves the problems that in the prior art, a used magnetic field probe with a fixed shape and a fixed size is greatly different from an actual human organ, and the magnetic field environment neglects the influence of human skin and in-vivo tissue fluid, so that measured magnetic field data is inaccurate.

The invention discloses application of dihydroartemisinin in construction of liver organoid, which is characterized in that by adding dihydroartemisinin into a liver organoid culture medium, the maturity of the liver organoid can be obviously improved, the maturity period is shortened, and the liver function characteristics (such as metabolic function and gene expression maturity) of the organoid are enhanced. The defects of an existing liver organ model in the aspect of simulating primary liver functions are effectively overcome, a more accurate in-vitro model is provided for research of liver related diseases, and a new platform and possibility are provided for development of liver regeneration and treatment methods.

The invention relates to the technical field of exosome application, in particular to application of an organoid exosome in preparation of a composition for treating renal injury. The organoid exosome is used for astragaloside drug loading (3D cultured exosome drug loading), and can be used for treating kidney injury and reducing cytotoxicity. Particularly, the effect of repairing the injury of a kidney injury organ model constructed by cis-platinum 100 [mu] M is more remarkable, and the cytotoxicity of an anoxic injury organ model is more remarkably reduced after drug-loading treatment treatment is carried out on the anoxic injury organ model.

This invention discloses a gene editing system for constructing a porcine nuclear transplantation donor cell model of atherosclerosis with double gene mutations in AF and its applications. The invention provides a kit comprising plasmid pKG-U6gRNA (APOE-E2-gRNA2), a target sequence binding region as shown in nucleotides 3-22 of SEQ ID NO: 25 (FBN1-gRNA4), a target sequence binding region as shown in nucleotides 3-22 of SEQ ID NO: 26 (FBN1-gRNA6), and FBN1-mutant-ss163 as shown in SEQ ID NO: 27. The kit is used for: preparing recombinant cells; preparing porcine atherosclerosis models; preparing atherosclerosis cell models, atherosclerosis tissue models, or atherosclerosis organ models. The plasmid pKG-U6gRNA (APOE-E2-gRNA2) is transcribed to obtain sgRNA with a target sequence binding region as shown in nucleotides 1-20 of SEQ ID NO: 11. APOE‑E2‑gRNA2 This invention lays the foundation for developing pig models of atherosclerosis through somatic cell nuclear transfer animal cloning technology.

This invention discloses a gene editing system for constructing porcine nuclear transplantation donor cells for cataract disease models with MIP gene mutations and its applications. This invention provides the application of MIP-gRNA2 (SEQ ID NO: 18), MIP-gRNA3 (SEQ ID NO: 19), and NCN protein in a preparation kit. This invention also provides a method for preparing recombinant cells, comprising the following steps: co-transfecting porcine cells with MIP-gRNA2, MIP-gRNA3, and NCN protein to obtain recombinant cells. The recombinant cells are recombinant cells with a mutated MIP gene. The kits described above are used for: preparing recombinant cells; preparing porcine cataract models; preparing cataract cell models, cataract tissue models, or cataract organ models. This invention has significant application value for the development of cataract drugs and for elucidating the pathogenesis of this disease.

The invention relates to the technical field of biological medicine, in particular to a construction method and application of a respiratory virus infected alveolar organ model. Comprising the following steps: infecting alveolar cell source single cells by using a virus inoculum, coating the infected alveolar cell source single cells with matrigel, and inoculating the infected alveolar cell source single cells into a first culture medium for three-dimensional culture to obtain the respiratory virus infected alveolar organ model. According to the invention, alveolar cells are creatively applied to model characterization of respiratory virus infection, and particularly, alveolar cell source single cells are subjected to infection treatment by adopting influenza A H1N1 virus, so that the constructed model can well simulate and characterize the infection process of influenza A virus in human lung organs; the method provides an experimental basis for drug research and development (such as high-throughput screening of anti-influenza virus drugs), viral disease pathogenesis research, research on defense mechanisms of naturally occurring virus infection or virus infection caused by experiments and the like, and has important guiding significance.

The application discloses a three-dimensional ultrasonic CT image reconstruction method based on a neural network agent model. The application constructs an anatomically real organ model, simulates a wave field by means of a numerical solver, and generates a multi-organ training data set; constructs and trains a strong scattering neural operator (S 2 NO) based on an iterative structure of a convergent Borel series, which is used to solve a partial differential equation with a large calculation domain and high oscillation; collects time-domain wave field observation data of a part to be measured of a patient, and converts the time-domain wave field observation data into frequency-domain wave field observation data in a set frequency range through Fourier transform; uses the trained S 2 NO as an agent model to perform a full waveform inversion algorithm and iteratively reconstructs ultrasonic CT images; the application reconstructs three-dimensional ultrasonic CT images of in-vivo clinical samples, and the speed is about 10 times that of a traditional ultrasonic CT full waveform inversion reconstruction algorithm, and an imaging quality similar to that of standard magnetic resonance imaging (MRI) is achieved.

The invention provides a brain organoid / encephalitis B virus infected brain organoid model and a construction method and application thereof, and belongs to the technical field of organoid. The invention provides the kit for constructing the brain organoid, the brain organoid is constructed on the basis of the kit, the construction method of the brain organoid is provided, and the constructed brain organoid model shows clear staged development characteristics, completely reproduces cell diversity in the human brain development process and forms ventricular-like structural characteristics. The encephalitis B virus infected brain organ model constructed on the basis of the obtained brain organs can be used for deeply analyzing the pathogenic process and related immune response characteristics of JEV infected human brain, researching and developing vaccines related to encephalitis B virus and screening antiviral drugs.

The application discloses a kit for constructing an ATM gene mutation ataxia-telangiectasia model pig nuclear transfer donor cell. The application provides a kit comprising ATM-gRNA1 shown in SEQ ID NO: 16, ATM-gRNA4 shown in SEQ ID NO: 17 and NCN protein. The application also provides a method for preparing a recombinant cell: co-transfecting a pig cell with ATM-gRNA1, ATM-gRNA4 and NCN protein to obtain a recombinant cell. The recombinant cell is a recombinant cell with ATM gene mutation. The kit is used for: preparing a recombinant cell; preparing an ataxia-telangiectasia model pig; preparing an ataxia-telangiectasia cell model or an ataxia-telangiectasia tissue model or an ataxia-telangiectasia organ model. The application has great application value for research and development of ataxia-telangiectasia drugs and revealing the pathogenesis of the disease.

The application discloses a kind of space measurement benchmark construction methods based on three-dimensional organ model, to solve the problem of lack of anatomical explainability and insufficient robustness to biological individual differences due to dependence on external morphology or overall registration in prior art.Methods include: collecting voxel data of target three-dimensional organ model and coordinates and topological relationship of internal inherent anatomical feature points;Based on anatomical prior knowledge, define a hierarchical benchmark space network containing topological layer, geometric constraint layer and coordinate system layer;The anatomical feature point set of target model is mapped and adapted to the benchmark network, and the coordinate transformation relationship is established.The application constructs the benchmark by taking the internal stable anatomical structure network as the skeleton, significantly improves the adaptability to morphological variation, gives the spatial coordinates clear anatomical semantics, and realizes the whole traceability of the measurement process, providing a reliable foundation for precision medical research and multi-source biological space data integration.

To provide a medical image processing device that allows a user to confirm anatomical structures such as blood vessels and nerves within an organ during surgery without burdens by performing display control of a 3D organ model, and a method of operating the same.SOLUTION: A 3D organ model acquisition unit 20 acquires a 3D organ model 25. A hard mirror image acquisition unit 21 acquires a hard mirror image 27 including an organ to be observed. A posture matrix estimation unit 22 estimates a posture matrix representing a posture of the organ to be observed included in the hard mirror image 27, in a three-dimensional coordinate system used for the 3D organ model 25. A display control unit 23 controls the display of the 3D organ model 25 on the basis of the posture matrix.SELECTED DRAWING: Figure 2

The invention discloses a thymic epithelial tumor bionic biological 3D printing organ-like model as well as a construction method and application of the thymic epithelial tumor bionic biological 3D printing organ-like model. The TETs tumor extracellular matrix composition from the tumor tissue of a patient is determined through proteomics analysis and is used for guiding the preparation of the photocuring bio-ink. The finally prepared biological 3D printing organoid supports primary TETs cell proliferation, and the reduction degree of biophysical characteristics and molecular characteristics of the organoid is obviously superior to that of a traditional matrigel culture organoid. Furthermore, on the basis of the bionic platform, the fact that the rubitedin is a powerful candidate drug for treating TETs is screened out. And some potential curative effect prediction biomarkers are further identified. According to the method, the biological 3D printing type organ model of the TETs is constructed through a biological material design strategy driven by proteomics data for the first time, and a standardized platform is provided for precise drug screening and mechanism exploration of the TETs.

The application relates to the technical field of biological tissue engineering, and discloses an open co-culture organ chip, which comprises one or more culture units, each culture unit comprises a center liquid storage hole in the form of a stepped blind hole; the bottom wall of the center liquid storage hole is provided with one or more culture holes, and the stepped surface of the center liquid storage hole is provided with a co-culture hole extending in the axial direction; and the center liquid storage hole comprises a column hole section protruding from the surface of the organ chip body. The co-culture organ chip is in an open form, the culture holes and the co-culture channel are located in a two-dimensional plane, and the openings are oriented to the same side, so that the operation difficulty is greatly reduced. Through standardized design of the arrangement and layout of the center liquid storage holes on the co-culture organ chip, the open operation channel is compatible with market operation and detection equipment, is more convenient, and is more suitable for industrial promotion. The application further discloses an application of the co-culture organ chip in construction of a multi-cell co-culture organ model.

The utility model relates to the technical field of tumor cell metastasis models, in particular to a breast cancer circulating tumor cell remote metastasis model which comprises a base and a fixing rod, the fixing rod is fixedly connected to the base, the fixing rod is fixedly connected with a brain metastasis model, a placing component is installed on the brain metastasis model, and the placing component is fixedly connected with the brain metastasis model. The placing part comprises an inclined plate, a fixed plate, a movable plate, a pressed plate, a straight spring and a collecting box, the inclined plate is fixedly connected in the brain metastasis model, the clamping plate clamps small balls with different colors through the matching effect of the extrusion rod, the movable plate, the collecting box and other parts, the small balls can be taken out by pulling the clamping plate, and the small balls can be conveniently taken out. And residual water is left in the collecting box, so that the small balls can be quickly taken out, meanwhile, no water drips on the outer surface of the model, the situation that the transparency of the model is affected by water marks generated on the outer surface of the model due to water dripping is avoided, and the transparency of the organ model and the cleanliness of a placement environment are guaranteed.

This invention provides a method for constructing an esophageal cancer biomimetic organ model based on a complex hydrogel and its use, belonging to the field of medical biomimetic organ models. The biomimetic organ model is manufactured using a hydrogel as its framework. This invention constructs an in vitro biomimetic organ model of esophageal cancer with bioactivity, better simulates Pg infection in esophageal cancer patients, and finally introduces commonly used chemotherapy drugs to confirm the relationship between Pg and malignant changes in esophageal cancer, verify the drug screening function of the model, and compare it with clinical treatment effects.

This invention discloses a method and its application for constructing a sickle cell anemia model pig with HBB gene mutations using nuclear transfer donor cells. This invention provides the application of HBB-gU1, HBB-gD3, HBB-mutant-ss174, and NCN proteins in a preparation kit. The kit is used for: preparing recombinant cells; preparing sickle cell anemia model pigs; preparing sickle cell anemia cell models, sickle cell anemia tissue models, or sickle cell anemia organ models. HBB-gU1 is an sgRNA, and its target sequence binding region is shown as nucleotides 3-22 of SEQ ID NO: 18. HBB-gD3 is an sgRNA, and its target sequence binding region is shown as nucleotides 3-22 of SEQ ID NO: 19. HBB-mutant-ss174 is a single-stranded DNA molecule shown in SEQ ID NO: 20. The NCN protein is a Cas9 protein or a fusion protein containing a Cas9 protein. This invention has significant application value for the development of drugs for sickle cell anemia and for elucidating the pathogenesis of the disease.

The utility model relates to the technical field of medical teaching models, in particular to a gynecological tumor teaching operation model which comprises a model base, a model shell is fixedly arranged on the upper end face of the model base, and two fixing mechanisms are fixedly arranged at the upper end and the lower end of the front end face of the model shell. A model inner cavity is formed in the model shell, a model block groove is formed in the model inner cavity, a blood vessel model block is embedded in the center of the model block groove, two kidney model blocks are embedded in the two sides, close to the upper end, of the model block groove, and an intestinal tract model block and a gynecological tumor model block are embedded in the positions, close to the lower end, of the model block groove. According to the utility model, the rubber sheet embedded into the embedding groove can be fixed through the fixing mechanism, a real operation environment can be simulated, and each organ model can be taken out for display and identification after the model shell is opened.

The application discloses a face soft tissue defect point cloud completion method based on a PF-Net, and belongs to the technical field of face soft tissue defect completion. The method comprises the following steps: S1, a data preparation stage: a data enhancement algorithm is designed to obtain a paired data set of incomplete input and defect labels; S2, a model construction stage: a dynamic graph convolution and a boundary attention mechanism are introduced into an encoder to optimize a point cloud completion network model PF-Net; S3, a model training stage: a training set is input into the PF-Net model for training; S4, a model testing stage: a test set is input into a generator for testing; and S5, a model inference stage: for clinical face soft defect point cloud data, multi-scale input data are acquired and completion is performed. The application realizes autonomous simulation design of face soft tissue defects, solves technical barriers in existing digital prosthetic technology, such as dependence on healthy side mirror image data or standard organ models, and solves the problem of unstable repair effect caused by dependence on doctor experience and aesthetic literacy.

The present invention provides a medical simulation for realizing efficient training by guiding the operation of an endoscope probe in the training of an endoscope using a medical simulator, and a training method using the medical simulator. In addition, the invention further provides a medical simulator which is miniaturized as much as possible and low in cost and a training method thereof, so that on-site training is facilitated. A medical simulator (10) is provided with a stomach (6), which is a hollow organ model in which the inside can be imaged through the tip (221) of an endoscope probe (22), and the stomach (6) is provided with first to eleventh guide sections (G1 to G11) for guiding the endoscope probe (22), the first to eleventh guide sections (G1 to G11) being respectively disposed corresponding to different first to eleventh specific sites (P1 to P11).

The invention relates to the technical field of biomedical engineering, in particular to a hair follicle organ chip device which comprises a sealing layer and a fluid layer located below the sealing layer. A medium inlet and a medium outlet are formed in the sealing layer; a culture medium runner and a culture chamber are arranged in the fluid layer; the medium inlet and the medium outlet are both connected with the culture medium runner; the culture chamber is arranged in the culture medium runner; a full-skin structure is arranged in the culture chamber; a micropore array is arranged on the full-skin structure; the micropore array comprises a hair follicle organ model. According to the hair follicle organ chip device provided by the invention, the hair follicle organs are cultured in the micropore array of the full-skin structure, and the sealing layer and the fluid layer are combined, so that a culture medium and gas can be introduced into the micropore array, the biological characteristics and the real growth environment of hair follicles can be better simulated, and the reliability of an experimental result is improved.

This invention discloses a CRISPR system for constructing pig nuclear transfer donor cells for cataract models with YAP1 gene mutations and its applications. The invention provides the application of YAP1-gRNA2 (SEQ ID NO: 18), YAP1-gRNA3 (SEQ ID NO: 19), and NCN protein in a preparation kit; the kit is used for: preparing recombinant cells; preparing pig cataract models; preparing cataract cell models, cataract tissue models, or cataract organ models. This invention uses CRISPR / Cas9 technology combined with dual gRNA editing to knock out the YAP1 gene, simulating the natural pathogenesis and genetic characteristics of cataracts, and obtaining single-cell clones with the YAP1 gene knockout, laying the foundation for later breeding pig cataract models through somatic cell nuclear transfer animal cloning technology. This invention has significant application value for the development of cataract drugs and elucidating the pathogenesis of this disease.

This invention discloses a modular microfluidic organ-on-a-chip and its organ model construction method, relating to the field of biomedical engineering technology. The modular microfluidic organ-on-a-chip includes a chip body, a first culture chamber, a second culture chamber, and a sealing component. The chip body has a culture hole with its top surface facing downwards. The culture hole passes through the middle of the first perfusion channel and the middle of the second perfusion channel in sequence. The first culture chamber can be inserted into or removed from the culture hole. The second culture chamber can be inserted into the culture hole and located at the top of the first culture chamber or removed from the culture hole. Therefore, the target samples on the first and second porous membranes can be easily obtained without destructive disassembly of the chip body, improving the accuracy and reliability of subsequent offline characterization results. The chip body, the first culture chamber, and the second culture chamber are independent of each other. The chip body can be reused, reducing the overall cost of the experiment and facilitating promotion and application.