10 results about "Organ Model" patented technology

The application discloses a kind of space measurement benchmark construction methods based on three-dimensional organ model, to solve the problem of lack of anatomical explainability and insufficient robustness to biological individual differences due to dependence on external morphology or overall registration in prior art.Methods include: collecting voxel data of target three-dimensional organ model and coordinates and topological relationship of internal inherent anatomical feature points;Based on anatomical prior knowledge, define a hierarchical benchmark space network containing topological layer, geometric constraint layer and coordinate system layer;The anatomical feature point set of target model is mapped and adapted to the benchmark network, and the coordinate transformation relationship is established.The application constructs the benchmark by taking the internal stable anatomical structure network as the skeleton, significantly improves the adaptability to morphological variation, gives the spatial coordinates clear anatomical semantics, and realizes the whole traceability of the measurement process, providing a reliable foundation for precision medical research and multi-source biological space data integration.

The application discloses a face soft tissue defect point cloud completion method based on a PF-Net, and belongs to the technical field of face soft tissue defect completion. The method comprises the following steps: S1, a data preparation stage: a data enhancement algorithm is designed to obtain a paired data set of incomplete input and defect labels; S2, a model construction stage: a dynamic graph convolution and a boundary attention mechanism are introduced into an encoder to optimize a point cloud completion network model PF-Net; S3, a model training stage: a training set is input into the PF-Net model for training; S4, a model testing stage: a test set is input into a generator for testing; and S5, a model inference stage: for clinical face soft defect point cloud data, multi-scale input data are acquired and completion is performed. The application realizes autonomous simulation design of face soft tissue defects, solves technical barriers in existing digital prosthetic technology, such as dependence on healthy side mirror image data or standard organ models, and solves the problem of unstable repair effect caused by dependence on doctor experience and aesthetic literacy.

This invention discloses a modular microfluidic organ-on-a-chip and its organ model construction method, relating to the field of biomedical engineering technology. The modular microfluidic organ-on-a-chip includes a chip body, a first culture chamber, a second culture chamber, and a sealing component. The chip body has a culture hole with its top surface facing downwards. The culture hole passes through the middle of the first perfusion channel and the middle of the second perfusion channel in sequence. The first culture chamber can be inserted into or removed from the culture hole. The second culture chamber can be inserted into the culture hole and located at the top of the first culture chamber or removed from the culture hole. Therefore, the target samples on the first and second porous membranes can be easily obtained without destructive disassembly of the chip body, improving the accuracy and reliability of subsequent offline characterization results. The chip body, the first culture chamber, and the second culture chamber are independent of each other. The chip body can be reused, reducing the overall cost of the experiment and facilitating promotion and application.

The present application relates to a kind of based on double-chamber hydrogel microsphere intestinal bionic organ model and its construction method, belong to biotechnology field.It is specifically double-chamber hydrogel microsphere with rough pit surface wrapped by intestinal barrier, construction method includes the following steps: the preparation of double-chamber hydrogel microsphere microfluidic device, preparation of rough double-chamber hydrogel microsphere with rough surface and load content, microsphere surface matrix glue modification and surface construction intestinal barrier.It is specifically applied to drug testing and the application research of microorganism and intestinal interaction.The intestinal bionic organ model prepared in the present application has simple manufacturing steps, short manufacturing time, low cost, and the subsequent culture maturation time is greatly shortened compared to other organoid models.The intestinal organ microsphere is expected to become a research platform for intestinal diseases, to promote the research of various intestinal disease treatment methods, and to help cope with the treatment challenges brought by various intestinal complex diseases.

An adaptive force feedback method based on individualized virtual laparoscopic surgery, a device, and a medium, relating to the technical field of adaptive force feedback computation. The adaptive force feedback method comprises: detecting whether a virtual surgical instrument stylus tip is in contact with an abdominal tissue / organ model, wherein the outermost layer of the abdominal tissue / organ model is coated with a surface membrane, and when the virtual surgical instrument stylus tip is in contact with the surface membrane, it is determined that the virtual surgical instrument stylus tip is in contact with the corresponding abdominal tissue / organ model; if the virtual surgical instrument stylus tip is in contact with the abdominal tissue / organ model, identifying the type of the abdominal tissue / organ model in contact, and switching to force feedback parameter settings of the corresponding abdominal tissue / organ model to perform force feedback computation; and detecting whether the virtual surgical instrument stylus tip penetrates the surface membrane and enters the abdominal tissue / organ model, and if yes, performing force feedback computation on the basis of the switched force feedback parameter settings, and performing scene rendering. The present application designs a method capable of adaptively switching force feedback parameters for the types of different tissues and organs, thereby improving the quality and realism of virtual surgery.

This invention discloses a gene editing system for constructing porcine nuclear transplantation donor cells for a metoprolol reductase deficiency model with SPR gene mutations and its applications. The invention provides a kit comprising SPR-gRNA1 (SEQ ID NO: 16), SPR-gRNA4 (SEQ ID NO: 17), and NCN protein. The invention also provides a method for preparing recombinant cells: co-transfecting porcine cells with SPR-gRNA1, SPR-gRNA4, and NCN protein to obtain recombinant cells. The recombinant cells are recombinant cells with a mutated SPR gene. The kit is used for: preparing recombinant cells; preparing porcine metoprolol reductase deficiency models; and preparing metoprolol reductase deficiency cell models, tissue models, or organ models. This invention has significant application value for the development of drugs for metoprolol reductase deficiency and for elucidating the pathogenesis of this disease.

The application discloses a gene editing system for constructing a progeria model pig nuclear transfer donor cell with a ZMPSTE24 gene mutation and application thereof. The application provides a kit comprising ZMPSTE24-gRNA2 shown in SEQ ID NO: 16, ZMPSTE24-gRNA4 shown in SEQ ID NO: 17 and NCN protein. The application also provides a method for preparing a recombinant pig cell: co-transfecting ZMPSTE24-gRNA2, ZMPSTE24-gRNA4 and NCN protein into a pig cell to obtain a recombinant pig cell. The recombinant pig cell is a recombinant cell with a ZMPSTE24 gene mutation. The kit is used for: preparing a recombinant pig cell; preparing a progeria model pig; preparing a progeria cell model or a progeria tissue model or a progeria organ model. The application has great application value for the research and development of progeria drugs and the revelation of the pathogenesis of the disease.

The application discloses an organ chip. The organ chip comprises a distribution layer, a porous membrane layer, a culture layer and a sensing layer which are sequentially stacked, the distribution layer is provided with a distribution flow channel, the distribution flow channel forms a feeding port on the distribution layer, the porous membrane layer is used for cell growth and is provided with micropores, the culture layer is provided with a culture unit and a communication hole, the culture unit is used for culturing organs or tissues, the micropores are communicated with the feeding port and the culture unit, and the sensing layer comprises a sensing electrode, and the communication hole is communicated with the culture unit and the sensing electrode. The porous membrane layer and the culture layer can culture cells and organs, realize construction of an organ model, the distribution layer can inject drugs, the sensing layer can monitor organ parameters in real time, and one-stop integration of culture, injection and detection functions is realized.

The application discloses a kind of in vitro directional induction lenticular lens organ by relying on stiffness controllable hydrogel and its construction method and application, technical scheme includes the following steps: (1) pluripotent stem cells are directionally induced and differentiated into primary ectoderm cell group;(2) prepare specific stiffness hydrogel and carry out surface cell adhesion treatment;(3) primary ectoderm cell is planted on the polyacrylamide hydrogel after washing and is induced into neural ectoderm cell group;(4) neural ectoderm cell is directionally differentiated into mature lenticular lens organ.The three-dimensional lenticular lens organ model obtained by the method has the top surface and the bottom surface of the spherical surface lenticular morphology, transparent and has capsule, lenticular lens epithelial cell, lenticular lens fiber cell and other structures, bottom surface is also completely wrapped by lenticular lens capsule.Compared with previous method, the formation of bottom surface complete capsule and spherical surface morphology is realized.

This document provides materials and methods for making and using functional (e.g., vascularized) organ models (e.g., pancreas models). For example, functional pancreas models including an ECM containing a plurality (e.g., two or more) of pancreatic islets, and a vascular network are provided.