129 results about "Organ Model" patented technology

The invention relates to an operation auxiliary system and method based on image processing, and the method comprises the steps: obtaining a preoperative medical image of a patient, collecting a multispectral imaging medical image of a surface structure, blood flow distribution and tissue elasticity of an operation region in real time, carrying out the image feature extraction after preprocessing, obtaining a preoperative image feature and an intraoperative multispectral image feature, and carrying out the image feature extraction; constructing a biomechanical organ model, updating tissue deformation parameters of the biomechanical organ model, performing space-time alignment on the intraoperative multispectral image features and the biomechanical organ model, generating a three-dimensional enhancement model including vascular topology, lesion boundary and elastic distribution, and superposing the three-dimensional enhancement model to a real-time surgical field image to obtain a real-time surgical field image. Marking the relative position of the surgical instrument and the risk area, generating an intervention signal when the instrument is close to a critical safety distance threshold value, and performing surgical operation by a doctor under assistance. Multi-dimensional intraoperative information is obtained through multi-spectral imaging to construct a high-precision dynamic biomechanical model, and precise navigation and active intervention are achieved in combination with the augmented reality technology.

An apparatus for synthetizing medical images, wherein the apparatus includes a process and a memory containing instructions configuring the processor to receive an organ model related to a patient's organ, identify a region of interest within the organ model, wherein identifying the region of interest includes locating at least a point of view on the organ model and determining a view angle corresponding to the at least a point of view, wherein the at least a point of view and the corresponding view angle define at least one field of view that include at least a portion of the organ model, and generate at least a medical image as a function of the region of interest using an image generator, wherein the at least a medical image captures an anatomical structure of the at least a portion of the organ model.

A method and system to make a modular microfluidic device in a miniaturized form for cell culture, co-culture, and organ-on-a-microfluidic device as in-vitro model of human organs within compartmentalized 3D structures is described. The modular microfluidic device enables co-culturing of heterogeneous cell assemblies that are adjacent or sequentially connected to promote enhanced cell-cell interaction. In vivo-like flow into and / or from one cell growth chamber to adjacent cell growth chamber is maintained by micro-engineered porous barriers i.e., porous walls and membranes. These porous barriers provide a tool for mimicking the paracrine exchange between cells in the human body. The system comprises of a set of microelectrodes for real-time and long-term monitoring and quantitative measurement of the impact of stimuli on the epithelial tissue permeability. The modular microfluidic system provides a platform technology for bio-fabrication of variety of miniaturized in-vitro models for mimicking the structure and function of single and multi-organ models.

There is provided a diagnostic support program capable of displaying movement of an organ. A diagnostic support program that analyzes images of a human organ and displays analysis results. The program acquires a plurality of frame images of a human organ and calculates a frequency characterizing a state of the organ based on each of the frame images. A phase difference between a waveform in a previously acquired organ model and a waveform corresponding to the calculated frequency is calculated. A signal is then output indicating the phase difference.

The organ chip comprises a culture membrane and an actuating membrane, a first culture chamber, a second culture chamber, an actuating chamber and a perfusion channel are further formed in the organ chip, the culture membrane divides the first culture chamber and the second culture chamber, the perfusion channel is communicated with the first culture chamber, and the actuating chamber is communicated with the second culture chamber. A communication port of the perfusion channel and the first culture chamber faces the culture membrane, preferably directly faces the culture membrane, the perfusion channel is used for connecting an external air source, the actuating chamber separates the second culture chamber and the actuating chamber, and the actuating chamber can deform in response to a pressure change of the actuating chamber so as to conduct the pressure change between the actuating chamber and the second culture chamber; further, the culture membrane is deformed. The organ chip can more truly simulate the respiratory movement process of pulmonary alveoli and the multi-directional shear stress generated by gas to cells, and is beneficial to meeting the research requirements of respirator-related lung injury.

The invention relates to the technical field of tumor biomedical engineering and organ chips, in particular to a vascularized gastric cancer organ chip and a preparation method thereof.The chip is composed of an integrated micro-fluidic main body, an annular micro-column array and an optical sealing film, and a central culture cavity is divided into a tumor area and a blood vessel area by micro-columns; the preparation method comprises the following steps: injecting a fibrous protein solution containing human umbilical vein endothelial cells and cancer-related fibroblasts into a vascular region, and adding thrombin for in-situ gelation to form a pre-vascularized network; the method comprises the following steps: mixing a patient-derived gastric cancer organ with a methacrylic acid esterified gelatin pre-polymerized solution, injecting the mixture into a tumor area, and carrying out photo-crosslinking immobilization; culturing for 7-14 days under the dynamic perfusion condition of a mixed culture medium to obtain a three-dimensional gastric cancer organ model containing a capillary network; the method is expected to be used for rapid, low-cost and high-throughput screening of chemotherapy or anti-angiogenesis drugs and accurate prediction of individual curative effects of patients.

The invention relates to a blood flow connection type multi-organ chip capable of being freely assembled and reused, the blood flow connection type multi-organ chip comprises a chip cover body, organ culture chambers, a chip matrix and a pressure-sensitive film, the chip matrix comprises a plurality of groups of organ culture units which are mutually independent, each group of organ culture units comprises at least two culture flow channels and a plurality of organ culture holes, and the culture flow channels are communicated with the pressure-sensitive film. The organ culture holes are sequentially distributed at intervals along the culture flow channel, the bottom end of each organ culture hole is communicated with the culture flow channel, each organ culture hole is used for accommodating an organ culture chamber, the culture flow channel is located at the bottom of the chip substrate, one end of the culture flow channel is communicated with the storage pool, and the other end of the culture flow channel is communicated with the connecting port; the chip cover body is arranged at the top of the chip base body and completely covers the storage pools and the organ culture holes, the pressure-sensitive film covers the bottom of the chip base body to seal the culture flow channels, and in each group of organ culture units, a circulation path is formed between at least two culture flow channels through the storage pools. The biological function of the human body is better simulated, and disease modeling and treatment strategy research are carried out.

The invention discloses a liver progenitor organoid culture medium and a culture method, and belongs to the technical field of organoid culture. The culture medium comprises a basic culture medium, Jagged-1, Dexamehysone, TGF (Transforming Growth Factor)-alpha, beta-mercaptoethanol, BMP4 (Bone Morphogenetic Protein 4), FGF2 (Fibroblast Growth Factor 2), specific addition factors Glutamax, HEPES (Hydroxyethyl PES), Nicotinamide, Penicillin and the like. The liver progenitor organoid is constructed by using the liver tissue for the first time, continuous growth and efficient proliferation of human liver progenitor cells in vitro can be promoted under the condition that gene editing is not carried out, meanwhile, the pluripotency of the liver progenitor organoid is maintained, and the consistency of specific marker expression is maintained; and a novel batch model is provided for the evaluation of hepatotoxicity at the present stage. From the long-term view, a foundation is laid for constructing a liver organ model with more advanced various differentiated cells.

The invention relates to a culture method of a pancreatic cancer organ model. The culture method comprises the following steps: cleaning tissues and chopping the tissues through a chopping device; transferring the chopped tissue into a centrifugal tube filled with tissue digestive juice for digestion, and collecting upper suspension and residual tissue precipitate; mixing the collected upper-layer suspension with the digested residual precipitate, and treating to obtain cell and tissue precipitates; embedding the tissues and the cells into matrigel; and after the matrigel is solidified, culturing tissue cells by using a corresponding organoid culture medium, and obtaining primary organoid tissues which are pancreatic cancer tissues. Due to the adoption of the technical scheme, the culture method of the pancreatic cancer organoid model can be used for stably culturing pancreatic cancer organoid with high success rate, and the invention also relates to a culture method of the pancreatic cancer para-carcinoma organoid model.

The invention discloses a self-adaptive force feedback method and device based on individualized virtual laparoscopic surgery and a medium, and belongs to the technical field of self-adaptive force feedback calculation. The self-adaptive force feedback method comprises the following steps: detecting whether a touch pen point of a virtual surgical instrument is in contact with an abdominal cavity tissue / organ model or not; wherein the outermost layer of the abdominal cavity tissue / organ model is coated with a surface film, and when the touch pen point of the virtual surgical instrument is in contact with the surface film, it is judged that the touch pen point is in contact with the corresponding abdominal cavity tissue / organ model; if the touch pen point of the virtual surgical instrument is in contact with the abdominal cavity tissue / organ model, identifying the type of the contacted abdominal cavity tissue / organ model, and switching to force feedback parameter setting of the corresponding abdominal cavity tissue / organ model for force feedback calculation; and detecting whether a touch pen point of the virtual surgical instrument penetrates through the surface film and enters the abdominal cavity tissue / organ model or not, if so, performing force feedback calculation based on the switched force feedback parameter setting, and performing scene rendering. According to the method, the force feedback parameters can be adaptively switched according to the types of different tissues and organs, and the quality and authenticity of a virtual operation are improved.

The invention provides an organ chip, a lung organ model, a construction method and a simulation method, the organ chip comprises a culture membrane and an actuating membrane, the organ chip is further provided with a first culture chamber, a second culture chamber, an actuating chamber and a perfusion channel, the culture membrane separates the first culture chamber and the second culture chamber, the perfusion channel is communicated with the first culture chamber, and the actuating chamber is communicated with the second culture chamber. A communication port of the perfusion channel and the first culture chamber faces the culture membrane, preferably directly faces the culture membrane, the perfusion channel is used for connecting an external air source, the actuating chamber separates the second culture chamber and the actuating chamber, and the actuating chamber can deform in response to a pressure change of the actuating chamber so as to conduct the pressure change between the actuating chamber and the second culture chamber; further, the culture membrane is deformed. The organ chip can more truly simulate the respiratory movement process of pulmonary alveoli and the multi-directional shear stress generated by gas to cells, and is beneficial to meeting the research requirements of respirator-related lung injury.

The invention belongs to the technical field of biomedical engineering and tissue engineering, and particularly relates to an acellular matrix modified bionic micro-fluidic organ chip constructed by 3D printing composite near-field direct writing as well as a preparation method and application of the bionic micro-fluidic organ chip. According to the bionic micro-fluidic organ chip prepared by the invention, the microstructure and physiological similarity of a model are remarkably improved, the problems of insufficient bionic property, single cell and the like at present are solved, and the preparation method is simple, is suitable for batch production and solves the problem of high production cost. The invention provides a new technical direction for the construction of the bionic organ model.

The invention relates to a patient-specific multi-material bionic tumor organ model and a preparation method thereof. The method comprises the following steps: carrying out separation and three-dimensional modeling on different anatomical structures in an organ of a patient based on medical image data through an image segmentation technology. And establishing a corresponding relationship between the medical image data and different anatomical structures in the organ of the patient based on a three-dimensional modeling result, and restoring three-dimensional information of the tumor. And performing biomechanical measurement on each biological tissue in the general specimen corresponding to the organ of the patient to obtain a biomechanical measurement result of each biological tissue. And performing structure layering on a three-dimensional modeling result based on a biomechanical measurement result, and mapping a plurality of layered tissue areas with a plurality of printing materials matched with biomechanical properties. And preparing an initial bionic model based on the selected printing material and the biomechanical measurement result corresponding to each biological tissue by adopting a 3D printing technology, and post-processing and assembling the initial bionic model to obtain the bionic tumor organ model.

The invention provides a method for evaluating drug enterotoxicity in vitro through an intestinal organ model, and relates to the field of biological medicine. Comprising the following steps: preparing six different culture media, culturing the hESC by using the different culture media, inducing the hESC to respectively perform directional endoderm cell differentiation, posterior intestinal bulb differentiation, intestinal organ differentiation and intestinal organ maturation, and evaluating the drug enterotoxicity by using a prepared intestinal organ model. The intestinal organ model prepared by the invention is similar to human intestinal cells in composition, can accurately reflect the toxic effect of the medicine in the physiological environment in the human body, can be repeatedly obtained in quantity, and avoids clinical risks and ethical constraints; the sensitivity of drugs with different enterotoxicity can be reflected through cell viability, and the method is suitable for rapid evaluation of in-vitro high-flux drug enterotoxicity.

To provide a system configured by combining diseased patient data generated from AI with a disease organ model formed by 3D printing, for supporting practical training in medical internship, or the like.SOLUTION: A system includes: means for creating and reproducing diseased patient data generated from AI and a disease organ model formed by 3D printing; and means for supporting practical training in medical internship, or the like.SELECTED DRAWING: Figure 1

The present invention relates to a medical hydrogel, and in particular to a hydrogel, a preparation method therefor and the use thereof. The preparation method comprises the steps of: completely dissolving polyvinyl alcohol in deionized water to obtain a component A in a clear state; dissolving acrylamide in deionized water, then adding N,N-methylene bisacrylamide and ammonium persulfate, and mixing same until uniform to obtain a component B; and mixing the component A and the component B at a volume ratio of 1:1 and stirring same until the mixture is completely clear and transparent, then adding Ag-ZnO nanoparticles, and finally dropwise adding a tetramethylethylenediamine solution to obtain a hydrogel prepolymer, and curing same to obtain the hydrogel. The hydrogel obtained in the present invention can be applied to an organ model for surgical training, so that the prepared organ model can more accurately simulate the properties of a biological soft tissue to make surgical training more realistic, has a better performance in terms of inhibiting bacteria, anti-fatigue, electric conduction, stretching, compression and the like, and can also be subjected to operations such as electrocoagulation and hemostasis, endoscopy, suturing and cutting.

The invention relates to the field of cell culture, in particular to a liver organ model culture device and method.The liver organ model culture device comprises a temperature control box and a box cover arranged on the temperature control box, a pressing column is slidably installed in the middle of the box cover in a penetrating mode, a column spring is wound around the outer side of the pressing column, and one end of the column spring is connected with the upper end of the pressing column; the other end of the column spring is fixed to the upper end of the box cover, a culture piece is arranged at the position, located on the inner side of the box cover, of the upper end of the temperature control box, a plurality of liquid changing holes are formed in the upper end of the box cover in a penetrating mode, a bearing frame is placed at the upper end of the box cover, and a plurality of liquid injection pipes are embedded in the bearing frame in a penetrating mode; a plurality of liquid suction pipes are embedded in the bearing frame in a penetrating mode, and the liquid injection pipes are located on the sides of the liquid suction pipes. According to the invention, the culture effect is improved, the liquid changing efficiency is also improved, and meanwhile, the gel matrix block is prevented from being damaged during liquid changing.

The invention discloses a three-dimensional ultrasonic CT image reconstruction method based on a neural network agent model. The method comprises the following steps: constructing an anatomical real organ model, simulating a wave field by virtue of a numerical solver, and generating a multi-organ training data set; constructing and training a strong scattering neural operator (S2NO) based on an iterative structure of convergence Born series, so as to solve a partial differential equation with a large computational domain and high oscillation; collecting time domain wave field observation data of a to-be-detected part of a patient, and converting the time domain wave field observation data into frequency domain wave field observation data in a set frequency range through Fourier transform; using the trained S2NO as an agent model, executing a full waveform inversion algorithm, and iteratively reconstructing an ultrasonic CT image; according to the method, a three-dimensional ultrasonic CT image of an in-vivo clinical sample is reconstructed, the speed reaches about 10 times that of a traditional ultrasonic CT full-waveform inversion reconstruction algorithm, and the imaging quality similar to the standard magnetic resonance imaging (MRI) resolution is achieved.

Embodiments of the present application relate to a surgical early warning method and device based on ultrasound image visualization, equipment and medium. The method comprises: extracting the corresponding organ surface from the preset voxel model according to the label corresponding to a plurality of preset organs; wherein the organ surface is composed of triangular facets; according to the set frequency, the ultrasound section is refreshed and drawn according to the pose of the ultrasound section; according to the positional relationship between the triangular facets constituting the organ surface and the pose of the ultrasound section, an organ model is drawn; in response to the user's simulated puncture operation on the organ model, the puncture needle is displayed according to the spatial position of the puncture needle. The technical scheme of the embodiments of the present application can realize the effect of improving the safety and accuracy of the operation.

The application discloses a gene editing system for constructing a muscle weakness disease model pig nuclear transfer donor cell with a DOK7 gene mutation and application thereof. The application provides application of DOK7-gRNA2 shown in SEQ ID NO: 16, DOK7-gRNA4 shown in SEQ ID NO: 17 and NCN protein in preparation of a kit. The application further provides a method for preparing a recombinant cell, comprising the following steps: co-transfecting a pig cell with DOK7-gRNA2, DOK7-gRNA4 and NCN protein to obtain a recombinant cell. The recombinant cell is a recombinant cell with a mutated DOK7 gene. The use of any of the above kits is to prepare a recombinant cell, to prepare a muscle weakness model pig, to prepare a muscle weakness cell model, a muscle weakness tissue model or a muscle weakness organ model. The application has great application value for research and development of muscle weakness disease drugs and revealing the pathogenesis of the disease.

The utility model provides a human body model-based low-frequency magnetic field test system, which comprises a human body model, a plurality of organ models and a data acquisition instrument are arranged in the human body model, each organ model is provided with a plurality of magnetic field probes, and the data acquisition instrument is arranged below the organ models and connected with the magnetic field probes. The human body model is filled with an electrolyte solution, and the organ model and the data acquisition instrument are both placed in the electrolyte solution. The magnetic field probe solves the problems that in the prior art, a used magnetic field probe with a fixed shape and a fixed size is greatly different from an actual human organ, and the magnetic field environment neglects the influence of human skin and in-vivo tissue fluid, so that measured magnetic field data is inaccurate.

Simulated tissue structures for practicing surgical techniques and methods of manufacturing those structures are provided. In particular, a realistic organ model or simulated tissue portion for practicing the removal of a tumor or other undesired tissue followed by suturing a remnant defect as part of the same surgical procedure is provided. The simulated tissue structures include a polyp simulation having a suturable mesh layer that is separable from a defect layer. A simulated colon model with interchangeable and suturable tissue pods is also provided as is a fully suturable rectum model and a rectum model with integrative suturable and removable polyp zones.

The invention discloses application of dihydroartemisinin in construction of liver organoid, which is characterized in that by adding dihydroartemisinin into a liver organoid culture medium, the maturity of the liver organoid can be obviously improved, the maturity period is shortened, and the liver function characteristics (such as metabolic function and gene expression maturity) of the organoid are enhanced. The defects of an existing liver organ model in the aspect of simulating primary liver functions are effectively overcome, a more accurate in-vitro model is provided for research of liver related diseases, and a new platform and possibility are provided for development of liver regeneration and treatment methods.

The invention relates to the technical field of exosome application, in particular to application of an organoid exosome in preparation of a composition for treating renal injury. The organoid exosome is used for astragaloside drug loading (3D cultured exosome drug loading), and can be used for treating kidney injury and reducing cytotoxicity. Particularly, the effect of repairing the injury of a kidney injury organ model constructed by cis-platinum 100 [mu] M is more remarkable, and the cytotoxicity of an anoxic injury organ model is more remarkably reduced after drug-loading treatment treatment is carried out on the anoxic injury organ model.

This invention discloses a gene editing system for constructing a porcine nuclear transplantation donor cell model of atherosclerosis with double gene mutations in AF and its applications. The invention provides a kit comprising plasmid pKG-U6gRNA (APOE-E2-gRNA2), a target sequence binding region as shown in nucleotides 3-22 of SEQ ID NO: 25 (FBN1-gRNA4), a target sequence binding region as shown in nucleotides 3-22 of SEQ ID NO: 26 (FBN1-gRNA6), and FBN1-mutant-ss163 as shown in SEQ ID NO: 27. The kit is used for: preparing recombinant cells; preparing porcine atherosclerosis models; preparing atherosclerosis cell models, atherosclerosis tissue models, or atherosclerosis organ models. The plasmid pKG-U6gRNA (APOE-E2-gRNA2) is transcribed to obtain sgRNA with a target sequence binding region as shown in nucleotides 1-20 of SEQ ID NO: 11. APOE‑E2‑gRNA2 This invention lays the foundation for developing pig models of atherosclerosis through somatic cell nuclear transfer animal cloning technology.

This invention discloses a gene editing system for constructing porcine nuclear transplantation donor cells for cataract disease models with MIP gene mutations and its applications. This invention provides the application of MIP-gRNA2 (SEQ ID NO: 18), MIP-gRNA3 (SEQ ID NO: 19), and NCN protein in a preparation kit. This invention also provides a method for preparing recombinant cells, comprising the following steps: co-transfecting porcine cells with MIP-gRNA2, MIP-gRNA3, and NCN protein to obtain recombinant cells. The recombinant cells are recombinant cells with a mutated MIP gene. The kits described above are used for: preparing recombinant cells; preparing porcine cataract models; preparing cataract cell models, cataract tissue models, or cataract organ models. This invention has significant application value for the development of cataract drugs and for elucidating the pathogenesis of this disease.

The invention relates to the technical field of biological medicine, in particular to a construction method and application of a respiratory virus infected alveolar organ model. Comprising the following steps: infecting alveolar cell source single cells by using a virus inoculum, coating the infected alveolar cell source single cells with matrigel, and inoculating the infected alveolar cell source single cells into a first culture medium for three-dimensional culture to obtain the respiratory virus infected alveolar organ model. According to the invention, alveolar cells are creatively applied to model characterization of respiratory virus infection, and particularly, alveolar cell source single cells are subjected to infection treatment by adopting influenza A H1N1 virus, so that the constructed model can well simulate and characterize the infection process of influenza A virus in human lung organs; the method provides an experimental basis for drug research and development (such as high-throughput screening of anti-influenza virus drugs), viral disease pathogenesis research, research on defense mechanisms of naturally occurring virus infection or virus infection caused by experiments and the like, and has important guiding significance.

The application discloses a three-dimensional ultrasonic CT image reconstruction method based on a neural network agent model. The application constructs an anatomically real organ model, simulates a wave field by means of a numerical solver, and generates a multi-organ training data set; constructs and trains a strong scattering neural operator (S 2 NO) based on an iterative structure of a convergent Borel series, which is used to solve a partial differential equation with a large calculation domain and high oscillation; collects time-domain wave field observation data of a part to be measured of a patient, and converts the time-domain wave field observation data into frequency-domain wave field observation data in a set frequency range through Fourier transform; uses the trained S 2 NO as an agent model to perform a full waveform inversion algorithm and iteratively reconstructs ultrasonic CT images; the application reconstructs three-dimensional ultrasonic CT images of in-vivo clinical samples, and the speed is about 10 times that of a traditional ultrasonic CT full waveform inversion reconstruction algorithm, and an imaging quality similar to that of standard magnetic resonance imaging (MRI) is achieved.

The invention provides a brain organoid / encephalitis B virus infected brain organoid model and a construction method and application thereof, and belongs to the technical field of organoid. The invention provides the kit for constructing the brain organoid, the brain organoid is constructed on the basis of the kit, the construction method of the brain organoid is provided, and the constructed brain organoid model shows clear staged development characteristics, completely reproduces cell diversity in the human brain development process and forms ventricular-like structural characteristics. The encephalitis B virus infected brain organ model constructed on the basis of the obtained brain organs can be used for deeply analyzing the pathogenic process and related immune response characteristics of JEV infected human brain, researching and developing vaccines related to encephalitis B virus and screening antiviral drugs.