Liver organ model and construction method and application thereof

Abstract

The invention discloses a liver organ model and a construction method and application thereof. The method includes steps: (1) subjecting embryonic stem cells to supported culture; (2) preparing cell balls, sequentially subjecting to differentiation culture through a mesoblast-entoderm stage, a liver induction stage and a mature stage to obtain different types of cells including liver cells, bile duct cells and endothelial cells, and subjecting the different types of cells to orderly arrangement and combination to form a liver organ. By control of exposure time of compounds in different differentiation stages, screening and evaluation of liver toxicity effects and liver early development influences of the compounds are carried out. In addition, by the liver organ model, high-flux short-timeevaluation of the liver development influences and the liver toxicity effects of one compound or various compounds can be realized, evaluation indexes are simple, direct and diversified, quick primary screening of a great quantity of new synthetic compounds can be realized, and a foundation is laid for subsequent compound modification, pharmacological and toxicological mechanism research or marketing for use.

Description

technical field [0001] The invention belongs to the cross field of cell biology and environmental toxicology, and relates to a liver organoid model and its establishment method and application. Background technique [0002] In recent years, due to the advancement of science and technology, the synthesis of new compounds and the extraction of plant active ingredients have become more convenient and efficient, but at the same time these emerging single compounds or mixtures also have potential risks to human health. On the one hand, new compounds have excellent properties and are often put into the market and used in large quantities quickly after development. On the other hand, before being put into the market, these compounds often lack strict health risk assessment data, and it is not clear whether they are harmful to the human body. The liver is an organ with the main metabolic function in the human body. Toxic substances produced in the body and ingested outside the body...

AI Technical Summary

Problems solved by technology

However, because the experimental cell types are cancer cell lines or primary cells, which are not healthy cells under physiological conditions and the experimental cell types are single, it is impossible to study the process of organ development, which greatly limits the breadth and depth of research.

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