32 results about "Mature stage" patented technology

The present application relates to the technical field of peanut variety creation and breeding, and particularly relates to a screening method of selenium-rich high-yield peanut core germplasm. The screening method comprises the following steps: using a selenium solution to culture peanut seeds to be screened, using peanut seeds to be screened cultured by sterile water as a control, screening peanut seeds with root length higher than the control by more than 10%, and obtaining selenium-sensitive peanut seeds; after sowing the selenium-sensitive peanut seeds, performing selenium treatment, using corresponding selenium-sensitive peanut seeds cultured by sterile water as a control, after one week of selenium treatment, screening peanut seeds with plant height and aboveground dry matter weight higher than the corresponding control, and obtaining selenium-rich peanut germplasm; sowing the selenium-rich peanut germplasm, using Zhonghua No. 16 as a control, screening peanut seeds with pod yield per plant higher than the control at the mature stage, and obtaining selenium-rich high-yield peanut core germplasm. The present application has the advantages of low cost, quantification, repeatability, high efficiency and easy implementation.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a method for improving drought resistance of rice. By knocking out the DRG1 gene and combining deep root ratio phenotype identification and seedling stage and mature stage drought stress phenotype identification results, when the gene segment is deleted, the deep root ratio of rice is reduced, the drought stress resistance is reduced, and the function and application approach of the gene are proved.

The invention provides bacillus methylotrophicus N7, a preparation and application of the bacillus methylotrophicus N7 to prevention and control of soybean cyst nematode, and belongs to the technical field of microorganisms. The preservation number of the bacillus methylotrophicus N7 is CGMCC (China General Microbiological Culture Collection Center) No.31391, and the bacillus methylotrophicus N7 is obtained by mutagenizing an original strain The strain is suitable for soybean low-temperature producing areas at 5-20 DEG C, has high soybean cyst nematode resistance, the cyst inhibition rate of a high-concentration group in the seedling stage is over 70%, the soybean yield in the mature stage is increased by 24.6% compared with a blank control, and the strain is non-toxic, free of residues and eco-friendly to soil.

This invention relates to the field of bioengineering technology and provides an application of the maize heat shock transcription factor gene ZmHSF18, wherein the nucleotide sequence of the maize heat shock transcription factor gene ZmHSF18 is shown in SEQ ID NO:1. Experiments have demonstrated that overexpression of the maize heat shock transcription factor gene ZmHSF18 in plants can significantly improve seed germination rate and frozen survival rate at the mature stage under low-temperature stress, reduce malondialdehyde accumulation, and increase superoxide dismutase activity. Therefore, this maize heat shock transcription factor gene ZmHSF18 can be used to improve plant cold tolerance and create transgenic plants with enhanced cold tolerance, possessing important application value in plant genetic engineering and stress resistance breeding.

The present application belongs to the poplar plantation technical field, and particularly relates to a poplar plantation structure optimization and sustainable management method and system. The method comprises the following steps: S1, constructing a growth model of poplar, and dividing the poplar into three stages of a juvenile stage, a middle-aged stage and a near-mature stage according to the growth model; S2, collecting site factor data; S3, dividing the research area into multiple site grade areas, and configuring differentiated growth parameters, rotation periods and felling intensities for the site grade areas; S4, building a multi-objective linear programming model, and the objective function covers maximization of total wood yield, minimization of management cost and maximization of ecological benefit; S5, linearly fitting the juvenile stage, the middle-aged stage and the near-mature stage to obtain the corresponding linear equations of the stages, embedding the linear equations of S2 into the multi-objective linear programming model as constraint conditions, and clearly defining the forest age range of each stage; and S6, solving the multi-objective linear programming model to obtain optimal felling area, rotation period and felling intensity parameters.

The present application belongs to the technical field of plant morphology, and particularly relates to a seed plant propagule three-dimensional shape measurement method based on a video stereomicroscope, comprising: collecting seed plant propagules at the mature stage and air-drying to a constant weight; classifying the propagules according to the seed, fruit shape and surface accessory structure, and determining the measurement criteria of the shape of different categories of propagules; scanning the three-dimensional structure of each category of propagule by using a video stereomicroscope, and accurately measuring the shape parameters length, width and thickness of the propagule; normalizing each category of propagule, calculating the shape index of the propagule, and representing the closeness to a spherical shape. The method quantifies the complex three-dimensional shape characteristics of the seed plant propagule into a comprehensive shape index, realizes accurate quantitative measurement and digitization of the morphological characteristics of the propagule, solves the pain point of determining the shape of the propagule by using a traditional observation tool, and facilitates the comparative identification of the three-dimensional shapes of different categories of seed plant propagules.

This utility model belongs to the field of passion fruit technology, and in particular to a climbing device for passion fruit cultivation. It includes an adjustable climbing structure with outward-expanding climbing structures on both the front and rear sides, and reinforced support structures on both sides. Through the adjustable climbing structure and the reinforced support structures, the reinforced support structures effectively support the adjustable climbing structure, ensuring its stability. Furthermore, the device possesses high flexibility and adaptability, allowing for precise adjustments based on the actual needs of passion fruit at different growth stages. Whether it's the initial climbing during the seedling stage or the vigorous growth during the mature stage, this structure provides just the right amount of support and guidance, not only helping the vines achieve even distribution throughout the growth process but also effectively preventing insufficient light caused by overly dense vines.

The invention particularly relates to application of an OsBB4 gene in illumination-dependent regulation of rice growth traits and yield traits. Wild type rice and OsBB4 silent homozygous mutant seeds are subjected to germination accelerating culture under the natural light condition and the weak light condition respectively, then seedlings are transplanted to an outdoor field for growth, photographing is carried out in the mature period, plant types are recorded, and the number of grains per ear and the thousand seed weight are counted. Results show that after natural light germination accelerating culture, the plant height, the number of grains per ear and the thousand seed weight of the mutant in the seedling stage and the mature stage are obviously lower than those of a wild type; after weak light germination accelerating culture, the plant heights of the two types have no significant difference in the mature period, but the number of grains per ear and thousand seed weight of the mutant are significantly higher than those of the wild type. According to the invention, the functional characteristics of the OsBB4 gene are clarified, a strategy for improving the gene to restore the plant type is provided aiming at the characteristic that the OsBB4 silent homozygous mutant responds to weak light, and finally the purpose of increasing the rice yield by increasing the number of grains per ear and the thousand seed weight is achieved. The invention provides important experimental basis and technical reference for rice high-yield cultivation optimization and molecular breeding.

The invention provides a method for monitoring the effectiveness of a rice blast-resistant gene in a field. The method comprises the following steps: selecting a rice blast-resistant single-gene variety, a hybrid rice parent / resistant source and a main cultivated variety as test varieties; after the test variety is sown in line, the to-be-induced variety and the susceptible control are fully diseased, and the seedling leaf blast levels of the other varieties are investigated; randomly arranging and planting the to-be-tested varieties in a field according to districts, and planting inducing varieties around the field; leaf blast resistance identification is carried out in the tillering stage, and neck blast resistance identification is carried out in the yellow ripening initial stage. Based on identification results of leaf blast and neck blast of a single-gene variety and a hybrid rice parent / resistance source in two consecutive years, obtaining a resistance gene effectiveness monitoring result; dNA of the main cultivated variety is extracted to detect the resistance gene, and the monitoring reliability of the method is verified by combining the resistance identification result. According to the invention, a specific rice material is used for monitoring in a regional representative field disease nursery, and effective rice blast resistance genes or combinations in rice breeding and production in a specific rice region can be directly screened out.

The invention discloses a tenebrio molitor pupa breeding technology and an eating method, and belongs to the technical field of tenebrio molitor pupae. The breeding process comprises the following steps: preparing feed by taking wheat bran, selenium-enriched yeast, selenium-enriched vegetables and traditional Chinese medicinal material powder as raw materials, and feeding tenebrio molitor larvae until the tenebrio molitor larvae grow to a mature stage; placing the mature larvae of the tenebrio molitor in a low-temperature environment of 0-12 DEG C for refrigeration; when fresh pupae are required to eat, the refrigerated mature tenebrio molitor larvae are recovered and pupated at the temperature of 25-35 DEG C, and the fresh pupae pupated within 15 min are obtained. According to the method, pupation centralization of the tenebrio molitor larvae is achieved, instant supply of fresh pupae is guaranteed, commercialization requirements are met, the nutritional quality of the fresh pupae is improved, the problem of side effects possibly caused by eating of the fresh pupae is solved, and the aim of conditioning the body of the tenebrio molitor pupae is achieved.

The application discloses a cadmium-polluted paddy field rice-shrimp co-cultivation method using sulfate-reducing bacteria, which comprises the following steps: step 1, spraying diluted SRB bacterial agent propagation liquid in the cadmium-polluted paddy field to be treated, then making the bacterial agent fully contact with the soil in the form of rotary tillage, and adding water in the paddy field to a depth of 5-10 cm, wherein the cadmium content in the cadmium-polluted paddy field to be treated is 0.40-1.50 mg / kg, and the pH of the cadmium-polluted paddy field ranges from 5.5 to 6.5; step 2, monitoring the redox potential of the soil in the paddy field until the redox potential value of the soil is monitored to be below-150 mV within a preset time period, and then performing rice transplanting operation; step 3, after 10-15 days of rice transplanting, putting Australian freshwater lobster fry with a body length of 2-5 cm; and step 4, harvesting rice and lobsters at the mature stage of the rice respectively. The application aims to significantly reduce the cadmium content in rice and the availability of soil cadmium through the sulfate-reducing bacteria and rice-shrimp co-cultivation system.

The application provides a methylotrophic bacillus N7, a preparation and application thereof in prevention and control of soybean cyst nematode, and belongs to the technical field of microorganisms. The methylotrophic bacillus N7 has a preservation number of CGMCC No.31391, and is obtained by mutagenizing an original strain N1 with 0.4% ethyl methanesulfonate. The methylotrophic bacillus N7 is suitable for a low-temperature soybean production area with a temperature of 5 to 20 DEG C, has high resistance to soybean cyst nematode, a cyst inhibition rate of a high-concentration group in a seedling stage is higher than 70%, a soybean yield in a mature stage is increased by 24.6% compared with a blank control, is non-toxic and non-residual, and is friendly to soil ecology.

The application discloses ZmRLR1 application of a gene and a coded protein in regulating corn root development. ZmRLR1 The nucleotide sequence of the gene is shown in the sequence table SEQ ID No: 1, and the protein sequence is shown in the sequence table SEQ ID No: 2. The number of lateral root primordia, the total length of lateral roots and the total lateral root density of the seedling stage of the ZmRLR1 protein overexpression transgenic plant are significantly increased, the number and size of the root system of the fifth layer of crown roots at the mature stage are significantly increased, and the weight of the rhizosphere soil is increased.

The application provides a fruit picking method, system and device based on target recognition and a storage medium, and relates to the technical field of computer vision.The application collects image data of a fruit to be recognized, performs preprocessing, constructs a fruit maturity prediction model by using a convolutional neural network, inputs the preprocessed image data into the model, outputs a maturity index of the fruit, further extracts key features in the fruit image, calculates the difference between the maturity stage and the fruit image features, generates a difference index, corrects the maturity index of the fruit, sets a maturity threshold, and judges whether the fruit has reached a picking standard.The application realizes accurate judgment of fruit maturity and automatic picking by combining image processing, feature data analysis and a machine learning model.

The invention relates to the technical field of plant biological breeding, in particular to application of OsRH11 protein and a coding gene thereof in controlling male reproductive development of rice. The rice OsRH11 gene is separated and cloned, and it is found that a rice plant with the OsRH11 gene knocked out is pollen-free abortion; the pollen fertility of the complemented plant line is recovered, and the single plant maturing rate in the mature period is close to that of a wild type. Therefore, the invention newly finds a gene, namely the OsRH11 gene, related to the male sterility of the rice. Meanwhile, the reason of rice male sterility caused by gene mutation is discussed, the molecular mechanism of rice male sterility pollen abortion is clarified, the cognition of people on the rice male sterility molecular mechanism is further deepened, and a new viewpoint is provided for the molecular regulation mechanism of rice male reproductive development. The application of the male sterility gene OsRH11 gene in genetic improvement of rice varieties has important practical significance for rice breeding workers.

The invention belongs to the technical field of plant genetic engineering, and discloses application of a rice OsHT gene in improvement of drought resistance of rice. Clustered regularly interspaced short palindromic repeats (CRISPR) mutant phenotype identification is carried out on candidate genes, and the OsHT gene for controlling rice drought response is successfully cloned. The drought stress phenotype identification results in the seedling stage and the adult-plant stage show that when the gene segment is deleted, the drought stress resistance of the rice is obviously enhanced, so that the function of the gene and the potential of the gene in practical application are proved.

The invention relates to a potato aeroponic staging regulation nutrient solution and an application method, and belongs to the technical field of soilless culture. According to the method, differential nutrient solution formulas are respectively designed according to nutrient demand characteristics of five growth stages (a germination stage, a seedling stage, a tillering stage, a tuberization stage and a mature stage) of potatoes, the pH value is dynamically regulated and controlled to be 5.5-6.5, the EC value is gradually increased and then slightly decreased according to the growth stages, and a precise atomization application strategy is matched. A CaCl2-KNO3-KH2PO4 triangular system is adopted in the tuberization period, the problem of calcium and potassium antagonism is effectively solved, calcium absorption of tubers is remarkably improved, and the tuberization rate is lower than 3%. Liquid drops are prevented from making contact with the blades through the directional atomization and physical isolation technology, it is ensured that chlormequat chloride residues are not detected out, and the advantages of being efficient, safe and easy to operate are achieved.

The invention discloses an application of a tobacco single copper oxidase-like protein gene NtSKU5, the nucleotide sequence of the tobacco single copper oxidase-like protein gene NtSKU5 is shown as SEQ ID NO.1, the tobacco single copper oxidase-like protein gene NtSKU5 comprises 1818 basic groups, and the gene is derived from tobacco. According to the invention, agronomic traits of an NtSKU5 gene edited plant and a control (untransformed) plant are investigated in a squaring stage and a mature stage under normal conditions, and the plant height, stem girth, waist leaf length, waist leaf width and leaf area of the NtSKU5 gene edited plant are all higher than those of the control (untransformed). In a word, the NtSKU5 gene is knocked out by utilizing a CRISPR / Cas9 mediated gene editing technology to obtain a gene editing plant which affects the growth and development of the plant, so that a genetic material and a theoretical basis are provided for the research on the single copper oxidase-like protein of the tobacco and the research on the growth and development of the tobacco.

The invention provides an application of a GmbetaCA11 protein and / or a GmbetaCA11 gene in promoting soybean growth and / or increasing soybean yield, and belongs to the technical field of plant genetic engineering. The Gm beta CA11 gene is a gene for coding soybean carbonic anhydrase. By enhancing the expression level of the GmbetaCA11 gene and increasing the accumulation amount of the GmbetaCA11 protein in soybean bodies, the soybean growth and development process can be effectively regulated and controlled, the soybean plant growth can be remarkably promoted, and the soybean yield related traits can be improved. Through overexpression of the GmbetaCA11 gene in soybeans, the plant height and yield can be significantly increased; the plant height and single plant yield of the transgenic plant in the mature period are obviously higher than those of a control plant, and the growth situation in the seedling period is also better than that of the control plant. According to the invention, the important function of the GmbetaCA11 gene in regulation and control of soybean growth and yield formation is defined, and the GmbetaCA11 gene has important theoretical value and application prospect in cultivation of new high-yield soybean varieties.

The invention discloses application of HG1 in regulating and controlling the heading stage, the mature stage and the plant height of a plant. The invention finds that the heading stage of the plant can be obviously delayed by a method for overexpressing or up-regulating the HG1 gene or homologous genes thereof or increasing the expression of the HG1 gene; by knocking out or down-regulating the HG1 gene or the homologous gene thereof, the heading period and the mature period of the plant can be obviously advanced, the plant height is dwarfed, and the lodging resistance of the plant is improved. The achievement of the invention has important enlightenment significance in the field of plant breeding, provides a new strategy and method for cultivating plant varieties with specific growth and development characteristics, and is helpful for promoting further development of plant biology and agricultural science.

This utility model discloses an integrated device for plant seedling maintenance, relating to the field of seedling maintenance technology. It includes a support frame, of which multiple supports are provided, and a ring-shaped support member is fixedly connected to the top of each support frame. A ring-shaped irrigation member is slidably connected to the center of the bottom of the ring-shaped support member. When it is necessary to adaptively adjust the irrigation height of the irrigation nozzle, the positioning pin on the outer wall of the sliding plate can be pulled outwards, separating its end from the positioning hole. After being pulled out, the ring-shaped irrigation member can be slid up and down along the guide groove and slide of the support frame according to actual needs until the irrigation nozzle is aligned with the target irrigation area. Then, the positioning pin is released, allowing it to be inserted into the positioning hole of the corresponding height under the action of a compression spring, quickly locking the height of the ring-shaped irrigation member. The irrigation height can be adjusted according to the root distribution characteristics of the seedling and mature stages of the plant.

The invention discloses a corn hybrid germplasm resource saline-alkaline tolerant system as well as a construction method and application thereof, and belongs to the technical field of agricultural biology. The corn hybrid germplasm resource saline-alkaline tolerance system comprises evaluation subsystems of a germination stage, a seedling stage and a mature stage; in the germination stage, a mixed saline-alkaline solution with a specific ratio is adopted for stress, and a vitality index, relative water content, root length, root dry weight and germination rate are screened as key indexes; saline-alkali soil potting stress is adopted in the seedling stage, and fresh leaf weight, SOD activity, POD activity, relative water content and chlorophyll content are screened as key indexes; in the mature period, through saline-alkali soil field tests, hundred-grain weight and single spike weight are screened as key indexes. And calculating a comprehensive evaluation value of saline-alkaline tolerance of each stage through principal component analysis and a membership function method, so as to realize accurate grading of germplasm resources. The invention provides a systematic and reliable technical scheme for identification and breeding of saline-alkaline tolerant germplasm of corn.

The invention discloses a method for preventing pod cracking and increasing yield in the rape harvesting process, and belongs to the technical field of rape harvesting methods. Regulating and controlling the environment at the later growth stage: keeping the relative water content of the soil within the range of 40-60% and keeping the relative water content stable through irrigation or drainage measures from 10-15 days before the rape enters the pod mature stage to harvesting; spraying an anti-shattering agent: spraying the anti-shattering agent after 80% or more of rape pods in the field enter a wax ripening stage and have white film layers; harvesting of mature oilseed rapes: using an oilseed rape combine harvester to directly harvest the oilseed rapes, and according to harvesting requirements, the height of a header of the harvester is set to be 8-32 cm, the rotating speed of a reel is adjusted to be 18-32 revolutions per minute, the rotating speed of a threshing cylinder is set to be 400-500 revolutions per minute, and the harvesting operation speed is controlled to be 1.4-2.6 kilometers per hour. By means of the mode, the anti-shattering agent is sprayed to the oilseed rape in the specific period, meanwhile, harvesting parameters are optimized, the yield loss is reduced, and the economic benefits of oilseed rape planting are improved.

The invention discloses a rice and shrimp co-culture method in a cadmium-polluted rice field by using sulfate reducing bacteria, which comprises the following steps: step 1, spraying a diluted SRB microbial agent propagation liquid in a cadmium-polluted rice field to be treated, then enabling the microbial agent to be in full contact with soil in a rotary tillage manner, and adding water into the rice field until the depth is 5-10cm, the cadmium content in the cadmium-polluted rice field to be treated is 0.40-1.50 mg / kg, and the pH range of the cadmium-polluted rice field is 5.5-6.5; 2, monitoring the oxidation-reduction potential of soil in the rice field, and carrying out rice transplanting operation until the oxidation-reduction potential value of the soil is monitored to be below-150 mV within a preset time period; 3, 10-15 days after the rice seedlings are transplanted, young Australian freshwater lobsters with the body length of 2-5 cm are put in; 4, harvesting rice and lobsters in the rice mature period. The invention aims to significantly reduce the cadmium content in rice and reduce the effectiveness of soil cadmium through the sulfate reducing bacteria and rice-shrimp co-cropping system. The method disclosed by the invention is supported by a national key research and development plan project (2022YFD1700103).

The application relates to the technical field of intelligent control, in particular to a greenhouse environment digitalization control method and device, wherein the method comprises the following steps: acquiring environment data and crop form data in a greenhouse; analyzing the crop form data to obtain a growth stage of crops, wherein the growth stage of crops comprises a seedling stage, a growth stage, a flowering stage, a fruiting stage and a mature stage; inputting the environment data and the growth stage of crops into a crop demand matching model to obtain a matching degree of the environment in the current greenhouse and the growth stage of crops; if the matching degree is greater than or equal to a target threshold, the environment does not need to be controlled; if the matching degree is less than the target threshold, generating an environment regulation strategy according to an NSGA-II algorithm, and generating an environment regulation instruction to control the environment change in the greenhouse according to the environment regulation strategy. Thus, the problems that the existing control system lacks consideration of the growth stage of crops and is insufficient in multi-target optimization capability are solved.

The invention provides bacillus velezensis HZ17 and application thereof, and belongs to the technical field of microorganisms. The invention provides bacillus velezensis HZ17, and the preservation number of the bacillus velezensis HZ17 is CGMCC (China General Microbiological Culture Collection Center) No.35127. The bacillus velezensis HZ17 provided by the invention can be independently used for preventing and treating the wheat stem rot, or the bacillus velezensis HZ17 can be combined with chemical agents such as JS399-19 to efficiently prevent and treat the wheat stem rot. The result of the embodiment shows that when the bacillus velezensis HZ17 is used for preventing and treating the wheat stem rot, the disease index of the wheat stem rot in the seedling stage can be remarkably reduced, and the number of wheat white ears and the disease index of the wheat in the mature stage can be remarkably reduced. Furthermore, the bacillus velezensis HZ17 can be combined with JS399-19 for use, so that the effect of preventing and treating the wheat stem rot can be better achieved.

The invention relates to the technical field of ciona ciona breeding, and particularly discloses a ciona ciona full-artificial breeding method which is carried out according to the growth stage and comprises the following steps: firstly collecting mature ciona ciona gametes for artificial insemination, and culturing fertilized eggs by using filtered seawater containing antibiotics in a specific ratio; then floating / swimming larvae are screened, cleaned, subjected to multi-round state judgment and antibiotic replacement and transferred to a breeding tank through suspension culture, water is replaced every day, and dead individuals are observed and removed; subsequently, according to different stages of larvae, adults, sexually mature adults and aged adults, the culture time, the feeding frequency and the bait dosage are adjusted; in the sexual maturity stage, self-fertilization embryos need to be collected in a specific photoperiod. By means of the method, ciona glabra can be efficiently cultivated, the survival rate of all stages is guaranteed, and technical support is provided for large-scale cultivation of ciona glabra.

Provided in the present invention is an oligosaccharide-based multifunctional plant immunomodulator. Specifically, the plant immunomodulator has a structure represented by formula I below. The plant immunomodulator of the present invention can not only regulate the growth and development of crops, but also induce crops to resist against biological stress and abiotic stress, thereby improving the yield and quality of crops. By using the plant immunomodulator of the present invention, not only can multiple plant growth and development processes be positively regulated, including promoting seed germination, seedling morphogenesis and plant vigor at the mature stage, thereby increasing the crop yield and fruit quality, but also improving the resistance of plants against biological stress (diseases, insects and weeds) and abiotic stress (a frozen or near-frozen soil environment, drought, waterlogging, salinity and alkalinity, extremely low / high temperature, etc.). In addition, the plant immunomodulator of the present invention has the advantages of being non-toxic, free of pesticide residues, environmentally friendly, incapable of inducing pesticide resistance in pathogens, etc.

The present application relates to the beer brewing technical field, specifically to a kind of beer fermentation equipment and process, equipment includes yeast activation tank, main fermentation tank, flavour modification tank and post-mature wine storage tank in turn, respectively corresponding yeast activation, main fermentation, flavour modification, low-temperature post-mature four fermentation stages, quantitative sterile conveying pump and sterile control valve are achieved between each tank body quantitative sterile conveying of material, and each stage can be independently regulated and controlled environmental parameter;Process includes yeast activation stage, main fermentation stage, flavour modification stage and low-temperature post-mature stage.In the present application, each tank body only bears single fermentation function, single-tank failure does not affect the operation of other processes, realizes the accurate control of yeast metabolic pathway, directional enrichment of flavouring material, the consistency deviation of batch flavour is small, and beer flavour level is more rich, which can be widely applied to the large-scale production of various types of beer, especially suitable for customized production of high-quality craft beer.

The invention provides a prevention and treatment method for a plum fruit blackheart disease, and belongs to the technical field of fruit tree cultivation. The invention provides a prevention and treatment method for plum fruit blackheart disease, which comprises the following steps: spraying a bactericide for five times in the early flowering stage, the flowering stage and the early maturation stage, namely spraying once in the early flowering stage, spraying twice in the flowering stage and spraying twice in the early maturation stage. After the method is used for prevention and treatment, the occurrence rate of the blackheart disease can be reduced to 10% or below, and the effect is remarkable.