54 results about "Inoculation methods" patented technology

The invention discloses a tremella aurantialba liquid strain automatic inoculation device and an inoculation method thereof.The inoculation device comprises an inoculation needle which is connected to a supporting rod in a liftable mode, a tremella aurantialba liquid strain can be contained in the inoculation needle, an inoculation hole is formed in the side face, close to the tail end of a needle head of the inoculation needle, of the inoculation needle, and a leakage piece is rotatably connected to the top end of the inoculation hole; the leakage sheet can close the inoculation hole; a flow guide inclined plane is arranged in the inoculating needle, and the bottom end of the flow guide inclined plane is propped against and fixed with the bottom end of the inoculating hole; the inoculation ball is adjacently arranged beside the inoculation needle, and the inoculation ball is connected to the supporting rod in a lifting mode; the conveying belt is used for placing fungus bags, and the fungus bags can be conveyed to the positions below the inoculation needles and the inoculation balls by the conveying belt and are automatically inoculated under the cooperation of the inoculation needles and the inoculation balls. According to the invention, batch and automatic injection work of the tremella aurantialba bacterial liquid is realized.

This invention discloses an image recognition-based method for assessing grape powdery mildew. By introducing standardized sample preparation and uniform inoculation methods, this invention reduces the interference of randomness and spatial heterogeneity of natural field infection on the assessment results, making the disease occurrence process more controllable among different materials, thereby improving the stability and comparability of phenotypic data. Simultaneously, image recognition and machine learning technologies are used to automatically identify and calculate the area of ​​powdery mildew-infected regions, avoiding the reliance on the experience of assessors in traditional manual visual grading. This enables a continuous quantitative description of the degree of powdery mildew infection, improving the consistency and accuracy of the assessment results. This invention constructs a large-scale image analysis workflow that can be used for batch processing, significantly improving the efficiency of disease assessment, reducing the workload of manual investigation, and making this method applicable to applications requiring the processing of large numbers of samples, such as germplasm resource evaluation and breeding material screening.

The invention discloses a Ustilaginoidea virens inoculant, a preparation method and application of the Ustilaginoidea virens inoculant in Ustilaginoidea virens inoculation, and belongs to the technical field of agricultural microorganisms. According to the method, two-step shake-flask culture is combined with the steps of filtering, centrifuging and spore germination, so that the ustilaginoidea virens inoculum taking germinated spores (GC) as the core is prepared, and the problems of hypha aging, low spore activity, asynchronous infection and the like in the existing inoculation method are effectively solved. The inoculant has a tender hypha structure and strong infection activity, and the inoculation efficiency and the pathogenesis consistency are remarkably improved. When being used for preparing transcriptome samples, metabolome samples and other omics samples, the strain avoids non-specific immune interference caused by PAMPs released by broken hyphae, ensures synchronous infection process, and is real and reliable in immune response. The invention provides a stable and efficient inoculation system with a clean background for the research on the ustilaginoidea virens-rice interaction mechanism, and has a good application prospect.

The utility model provides a strain fermentation tank inoculation device which comprises a barrel and a flame ring, the barrel can be arranged on an inoculation opening in a sleeving mode, a sleeve is fixed to a fermentation tank, a net ring is fixed to the inner wall of the barrel, the flame ring is annularly fixed to the upper end of the net ring, and a conical cover integrally formed with the barrel is arranged at the upper end of the barrel. The top of the conical cover is provided with a through hole capable of pouring bacterial liquid for inoculation through the conical flask. According to the inoculation device for the strain fermentation tank, when a flame inoculation method is used for strain inoculation, flames generated by igniting the flame ring are limited in the barrel, the effects that the flames cover the inoculation opening to keep a sterile environment and prevent infectious microbes from entering the fermentation tank are achieved, diffusion of surrounding flames is limited through the barrel and the conical cover, and the inoculation effect is improved. And the risk that operators are scalded is reduced.

The invention provides a spore solid bacterium inoculation method. Relates to the technical field of edible mushrooms. The method comprises the following steps: S1, culture medium group preparation: selecting trees and setting a spore source culture medium group and a receptor culture medium group in groups; s2, inducing the spore source culture medium group to form a mature sporocarp, inoculating a target edible mushroom strain on the spore source culture medium group, inducing the strain to grow and forming the mature sporocarp; s3, controlling environmental parameters to promote spores to eject and fall on the receptor culture medium group; s4, the sporocarp is obtained through natural culture, the spore source culture medium group accounts for 5%-20% of the total culture medium group in volume, the surface of the receptor culture medium group in the S1 needs to be subjected to scribing treatment, the depth of a notch is 2-5 mm, the condition that the notch makes contact with the xylem is adopted as the criterion, and if the length of the tilia wood is smaller than 30 cm, scribing is not needed. The spore solid bacterium inoculation method provided by the invention has the advantages of reducing the planting cost and improving the economic benefit.

The invention relates to an automatic inoculation method and device for a pouring method in microbiological counting, and belongs to the field of microbiological devices.The automatic inoculation method comprises the following steps that a plurality of culture dishes added with sample liquid are placed in a lower dish channel and transferred to a dish receiving station; in the dish receiving station, the bottoms of the culture dishes are moved downwards to a filling disc through a guide cylinder material plate I, and the filling disc transfers the culture dishes to the inoculation station; in the inoculation station, a culture medium is injected into the culture dish bottom, after a filling disc swings and shakes clockwise and anticlockwise in a reciprocating mode, a control wheel drives the culture dish bottom to rotate, and the culture dish bottom enters a transition station after rotating; the transition station is used for transferring the culture dishes into the code printing station; the bottom of the culture dish is coded and transferred to the stacking station; after the bottoms of the culture dishes are supported and buckled with the culture dish covers, the culture dishes are dragged into the stacking channels, and through the steps, dish receiving, inoculation, transition, code printing and stacking of the culture dishes in the multiple dish discharging channels are completed. Human factor interference in the inoculation process is reduced to the greatest extent, and the inoculation process of a pouring method can be efficiently completed.

The invention discloses a virus-mediated plant high-flux mixed pool gene editing method which comprises the following steps: designing corresponding sgRNA according to a target gene, and adding a joint AAAC / TAGA connected with a carrier PaqC I restriction enzyme cutting site at two ends of the sgRNA; the method comprises the following steps: annealing designed sgRNA to synthesize double strands, and connecting the double strands to a carrier skeleton pGY345 subjected to PaqC I enzyme digestion through a T4 enzyme to construct a mixed library of sgRNA expression vectors; sgRNA expression vectors and TRV1 vectors in the mixed library are transformed into agrobacterium tumefaciens, a TRV virus vector mixed solution carrying different sgRNAs is constructed, and spraying inoculation is carried out; and observing the movement condition of the virus vector of the inoculated plant, and completing plant sgRNA typing and editing identification. By optimizing the OD value of the infection liquid and the inoculation method, the coverage degree of editing targets is remarkably improved, and multi-target mutant population creation can be carried out at the same time.

The invention discloses an inoculation method for seed embryos in green-skinned fruits of thin-skinned walnuts, and belongs to the technical field of plant tissue culture. Healthy green-skinned fruits of thin-skinned walnuts are selected, washed and disinfected with alcohol with a mass concentration of 75%, and in a clean bench, part of the green skin is cut off to expose the seed shell, and the seed embryos without cotyledons are inoculated into the culture medium after prying. On the 28th day of culture, the contamination rate, browning rate, and brown death rate are all 0, the survival rate is 100%, and the seed embryos grow well. The invention does not use highly toxic reagents such as mercuric chloride and irritating sodium hypochlorite for disinfection, the disinfection reagent does not contact the seed embryo, and after disinfection, it is not rinsed with sterile water, and it is not necessary to remove all the green skin and clamp the seed shell during inoculation; it saves time, labor, water, reagents, and costs, is safe, environmentally friendly, high-quality and efficient, is suitable for scientific research and factory-scale tissue culture seedlings, and lays a technical foundation for walnut subculture proliferation and the establishment of a leaf regeneration system for genetic transformation and gene editing to cultivate new varieties, and can also be used for the inoculation of seed embryos of other plant fruits.

The invention discloses a novel indoor identification method for the ringspot virus resistance of a new transgenic papaya variety, and belongs to the technical field of plant virus inoculation. According to the method, a sterile steel nail brush is used for dipping inoculation liquid containing ringspot viruses, indoor cultivated papaya leaves are inoculated, cultivation is conducted for 10 days after inoculation is completed, disease investigation is conducted on transgenic papaya, and the transgenic papaya is obtained according to the disease index of the inoculated transgenic papaya. And identifying the resistance of the transgenic papaya to the ringspot virus. The inoculation method adopted by the invention is simple and convenient to operate, has the advantages of high inoculation efficiency and good repeatability, can realize rapid batch inoculation of the ringspot virus on the papaya, and is not only suitable for screening new varieties of transgenic papaya resistant to ringspot, but also suitable for screening new varieties of transgenic papaya resistant to ringspot. And the method also has important significance for exploring the pathogenic mechanism of the ringspot virus and the disease-resistant mechanism of disease-resistant papaya varieties.

Vaccination methods include applying to a subject a microneedle patch having microneedles including a vaccine formulation including one or more infectious disease antigens. Infant and child subjects were found to tolerate application of the patch for a period effective to administer the antigen. The patch may be applied to the skin of a subject for an application period of five minutes or less, and effective to administer the antigen to the subject, wherein at least 50% of the height of the microneedle dissolves into the skin of the subject during the application period. Thermally stable vaccine formulations for the microneedles are provided. The methods may be effective to protect the subject from the infectious disease even if, prior to the applying step, the drug delivery device is transported or stored out of cold chain conditions for up to 14 days.

The invention provides an edible mushroom planting strain inoculator and an inoculation method, and relates to the technical field of strain planting.The edible mushroom planting strain inoculator comprises a base, a conveying belt is arranged on one side of the upper surface of the base, a mounting frame is fixedly mounted on the other side of the upper surface of the base, and an air cylinder is fixedly mounted on one side of the mounting frame through a connecting plate. A user places a planting strain on the surface of a conveying belt, then the conveying belt is started, the conveying belt conveys the planting strain to the position below a corresponding injector, after the planting strain is conveyed to the position below the corresponding injector, a worker moves a sliding block according to the length of the planting strain, and the sliding block drives the injector to move in the direction of a through groove; workers can conveniently move the injector according to the lengths of the planted strains, so that the injector is suitable for strains of different lengths or types, and frequent replacement or adjustment of equipment is not needed.

The utility model discloses a microorganism inoculation device applied to plant leaves, which comprises a plurality of fitting protection pieces, round bulges are arranged in the fitting protection pieces, accommodating cavities are formed in the bottoms of the round bulges, and disinfection cotton balls and target fungus cakes are sequentially arranged in the accommodating cavities from top to bottom. And two mounting grooves are formed in the bottom of each attaching protection piece, and high-temperature-resistant adhesives are arranged in the two mounting grooves. The utility model mainly has the following advantages: 1, the requirement on the inoculation environment is not too high, and the inoculation can be carried out under the conditions of high temperature and high humidity; 2, the inoculation method realizes the effects of wind resistance, high temperature resistance and rain resistance; 3, one leaf can realize inoculation of different strains or a plurality of fungus cakes of the same strain; 4, only the positions for inoculating the fungus cakes need to be covered with plastic, so that the fungus cakes are light-permeable and breathable, and field planting and disease attack are facilitated; and 5, the inoculation method is simple, and a plurality of operation steps can be completed indoors.

The invention relates to the technical field of plasmodiophora brassicae inoculation, in particular to an artificial inoculation method for plasmodiophora brassicae immediate infection and application, and the method specifically comprises the following steps: S1, accelerating germination: accelerating germination of seeds to obtain seedlings; s2, grinding bacteria and preparing a bacteria solution; s3, inoculation: performing field planting on the seedlings obtained in the step S1, and then performing injection inoculation on the overground parts of the seedlings by using the bacterial liquid prepared in the step S2; s4, seedling stage management; s5, disease investigation. According to the method, the cognitive limitation of traditional two-step infection of root hair infection and cortex diffusion is broken through, a brand-new inoculation technical path is constructed, and a new idea and method for inoculating plasmodiophora brassicae of cruciferae are developed; the defects that a traditional underground inoculation method is fuzzy in infection time sequence, uncontrollable in inoculation metering, low in inoculation efficiency and poor in pathogenesis stability due to large bacterial quantity fluctuation are fundamentally overcome.

This invention provides an inoculation device and method for edible fungi spawn, belonging to the technical field of edible fungi production equipment. The inoculation device and method include a frame and a conveyor. The conveyor transports the spawn. A pressing plate is slidably mounted on the frame, and the pressing plate has inoculation holes corresponding to the positions of the spawn. An elevator is mounted on the frame, and a receiving plate is installed at the bottom of the elevator. A needle corresponding to the position of the inoculation hole is located at the bottom of the receiving plate. Inside the pressing plate is a cleaning mechanism consisting of a toothed ring, brush bristles, transmission gears, drive gears, a drive sleeve, and a threaded rod. The linear motion of the receiving plate's lifting and lowering is converted into the rotational motion of the toothed ring, automatically brushing and cleaning the outer wall of the needle during needle piercing and retraction. This effectively removes residual culture medium, avoids needle clogging and cross-contamination, eliminates the need for manual cleaning during machine downtime, and significantly improves continuous operation time and inoculation quality.

The invention relates to a method for rapidly and stably identifying rice blast resistance indoors and application, and belongs to the technical field of plant disease prevention and control. The invention provides a plum culture medium which takes water as a solvent and comprises 30-60 mL / L of prune juice, 3-10 g / L of alpha-lactose, 0.5-2 g / L of yeast extract and 10-20 g / L of agar powder. The pH value of the plum culture medium is 6.5 to 7.5. According to the inoculation method disclosed by the invention, the inoculation success rate and the scab appearing degree of the pyricularia oryzae are remarkably improved by optimizing the key steps of seed soaking and germination acceleration, strain activation and propagation, conidium suspension preparation, spray inoculation and the like, and the inoculation method has the advantages of low cost, simplicity and convenience in operation, high standardization degree, good inoculation effect, small batch difference and high repeatability.

The invention belongs to the technical field of plant gene function research, and particularly relates to a method for inducing endogenous gene silencing of common tobacco (Nicotiana tabacum) by using a tobacco embrittlement virus. In order to solve the problems of unstable silencing efficiency, poor systematic silencing effect and the like of the existing TRV-VIGS technology in common tobacco, various key parameters about tobacco varieties, virus inoculation methods and plant culture conditions in the implementation process of the TRV-VIGS technology are determined, and the problems of common tobacco are effectively solved by adopting the method provided by the invention. The endogenous NtPDS gene of common tobacco is successfully silenced, and the silencing efficiency is as high as 99.02%.

The invention discloses an in-vitro high-throughput inoculation method of tea tree colletotrichum gloeosporioides, and belongs to the technical field of tea tree biology. The method comprises the following steps: preparing a spore suspension with the concentration gradient (absorbance) OD600 of 0.4, 0.6, 0.8 and 1.0 from the Colletotrichum calamiae, inoculating the spore suspension to an in-vitro tea tree leaf in an injection manner, then placing the leaf in a high-throughput microenvironment culture device, and culturing for 4 days under the conditions of 28 DEG C, 75% relative humidity and 12h illumination / 12h darkness, thereby accurately evaluating the infection efficiency of the pathogenic bacteria. According to the method, a standardized inoculation process is combined with high-throughput microenvironment control, so that the problems of long period, non-uniform environment and low efficiency of a traditional method are solved, and the method has the outstanding advantages of short inoculation period, good experimental result consistency and capability of realizing batch high-throughput screening; the method can be directly used for tea tree colletotrichum gloeosporioides resistance identification, pathogen toxicity analysis and bactericide screening.

The invention belongs to the field of plant disease infection, and discloses a quantitative inoculation method for infecting fungal diseases from plant wounds and application of the quantitative inoculation method. The quantitative inoculation method for infecting fungal diseases from plant wounds comprises the following steps: S1, formation of plant micro wounds: spraying quartz sand to host plant tissues by adopting a quartz sand spray gun, forming a plurality of micro wounds on the surfaces of the host plant tissues, and shaking off quartz sand grains on the plant tissues; and S2, spraying the fungal spore suspension to the leaf surface of the injured host plant, sealing the inoculated plant tissue, culturing to keep a water film on the surface of the inoculated leaf, and culturing until the plant tissue is successfully infected by fungi. The quantitative inoculation method can be applied to plant fungal disease research and plant fungal disease control method formulation. According to the quantitative inoculation method, the number of the micro-wounds formed on the surface of the plant tissue is controllable, counting is convenient, repeatability is good, and applicability is high.

The invention belongs to the field of crop disease resistance identification. The invention discloses a method for accurately evaluating the resistance of a corn variety to fusarium verticillium ear rot. The method comprises the following steps: inoculating corn ears by adopting a needling inoculation method, harvesting, threshing and grinding the ears in a harvesting period, measuring the content of fumonisins in grains, and evaluating the resistance of a corn variety to fusarium verticillium ear rot through the content of fumonisins in the grains. According to the method, the resistance of the corn variety to the fusarium verticillium ear rot can be evaluated more accurately, and the corn variety harmful to human and livestock is eliminated, so that the food safety is guaranteed.

The invention relates to a greenhouse fungus soil inoculation method for fusarium graminearum stem rot of corn, which comprises the following steps: activating fusarium graminearum strains, cutting hypha blocks at the edges of bacterial colonies, and inoculating the hypha blocks into a mung bean soup culture medium to prepare fusarium graminearum spore suspension; inoculating the spore suspension to sterilized corn kernels to prepare fusarium graminearum corn kernel inoculums; preparing bacterial soil, mixing bacteria and sowing in a greenhouse, performing disease grade investigation and resistance evaluation after 20 days, calculating the average disease grade of all plants of the to-be-identified corn material, and determining the resistance of the to-be-identified corn material to the fusarium graminearum stem rot. The inoculation method does not depend on a field environment, is short in inoculation period, high in efficiency and stable in result, can be combined with a field inoculation test to assist in verifying the resistance of a corn material to stem rot, and can be used for identifying and evaluating the disease resistance of a corn hybrid or a selfing line material.

The invention relates to the technical field of plant and microorganism interaction. The invention provides a method for inoculating camellia oleifera seedlings by using endophytic fungus piriformospora indica, which comprises the following steps of: inoculating an endophytic fungus piriformospora indica spore suspension into camellia oleifera seedling culture substrates of different depth layers in 2-3 times, keeping the substrates wet, and regularly weeding and preventing and treating diseases and insect pests. The invention also provides application of the endophytic fungus piriformospora indica in promoting the growth of camellia oleifera, improving the quality of camellia oleifera and improving the physicochemical properties of camellia oleifera soil. The invention further provides a layered inoculation method which can improve the colonization rate and growth promoting effect of oil tea root systems and remarkably improve the growth performance of oil tea seedlings. The colonization rate and the growth promoting effect are kept stable in different environments, and the method is suitable for large-scale field popularization.

The invention provides a soybean anthracnose inoculation method and application. The soybean anthracnose inoculation method comprises the steps of soybean colletotrichum gloeosporioides culture, soybean sprout culture and soybean colletotrichum gloeosporioides living body inoculation. The method comprises the following steps: cultivating soybean sprouts, cutting off roots of the soybean sprouts, inoculating stems of the soybean sprouts, putting the stems into a cryopreservation tube filled with wet degreasing cotton balls after inoculation, culturing at the temperature of 25 DEG C in darkness for 3 days, and counting morbidity according to established disease grading standards. The soybean anthracnose inoculation method provided by the invention has the characteristics of short period, simple and efficient operation steps, high accuracy and strong applicability, and provides technical support for soybean variety anthracnose resistance identification and in-vivo inoculation determination of the control effect of a bactericide.

The invention discloses a fishing vaccine continuous soaking inoculation method based on the Archimedes screw principle. The fishing vaccine continuous soaking inoculation method comprises the steps that fish schools and vaccine soaking liquid are synchronously conveyed to a cavity formed between a soaking cylinder and an oxygen pumping shaft pipe through a sliding way; the oxygen pumping shaft pipe and the soaking cylinder are divided by the spiral blades to form independent cavities, so that fish schools with proper density are continuously propelled and uniformly soaked in compartments according to a set propelling speed; the oxygen dissolving level of the vaccine soaking solution is dynamically maintained through the oxygen pumping shaft tube; the fish school state is monitored in real time based on an image acquisition device, and abnormal individuals are marked; the vaccine soak solution is recycled through the separation grid plate and the recovery system. The problems that traditional artificial inoculation is large in batch difference, low in inoculation efficiency, unstable in dissolved oxygen and large in fish school stress are solved.

The invention discloses a negative pressure fixation and micro-injection combined cotton virus-induced gene silencing inoculation method. The method comprises the following steps: (1) constructing a virus-induced gene silencing vector; (2) preparing an agrobacterium suspension; (3) fixing under negative pressure; (4) performing micro-injection inoculation; and (5) subsequent culture: culturing the inoculated plant under the conditions that the temperature is 22-25 DEG C, the illumination period is 16h illumination / 8h darkness, the illumination intensity is 100-200 and the relative humidity is 60-80%, and observing the gene silencing phenotype after 7-14 days. The method solves the problems that a traditional virus-induced gene silencing inoculation method is complex in operation, low in success rate and large in leaf damage, has the advantages of being easy and convenient to operate, high in inoculation success rate, small in leaf damage, high in gene silencing efficiency and the like, and is suitable for cotton functional genomics research and gene function verification.

The invention belongs to the technical field of plant fungus inoculation, and particularly relates to an inoculation method for colonizing a new host plant through endophytic fungi. The method specifically comprises the following steps: S1, inoculating endophytic fungi into a PDA culture medium, and culturing in a mould incubator for 24-26 days to obtain endophytic fungi hyphae; s2, placing the disinfected seeds in a water agar culture medium, and accelerating germination in an incubator to obtain plant aseptic seedlings; s3, when the aseptic seedlings of the plants grow to 3-5cm, scratching the aseptic seedlings of the plants, stuffing endophytic fungus hyphae into wounds, continuously placing the aseptic seedlings of the plants in the water agar culture medium, and culturing the aseptic seedlings of the plants in an incubator; and S4, planting the plant aseptic seedlings cultured for 3-7 days in the step S3 into aseptic soil, and observing whether hyphae exist in the plants or not after one month. In the technical scheme provided by the invention, endophytic fungi which cannot infect roots can use the method provided by the invention; efficiency and success rate are high.

The invention discloses a method for identifying bacterial wilt resistance through leaf injection inoculation in a solanaceae crop seedling stage. The method specifically comprises the following steps: (1) preparing solanaceae crop seedlings; (2) preparing a ralstonia solanacearum suspension; (3) performing leaf injection inoculation; and (4) identifying the bacterial wilt resistance. According to the method, the ralstonia solanacearum suspension is injected into the leaves from the wounds of the leaves, the inoculation method is rapid, efficient, easy and convenient to operate, stable and reliable in result and capable of being implemented on various solanaceae crops, and a new means is provided for resistance identification of disease-resistant germplasm resources of the solanaceae crops on a large scale.

The present application relates to the technical field of tobacco biocontrol bacteria inoculation, and particularly relates to a tobacco biocontrol bacteria inoculation method.The present application provides a tobacco biocontrol bacteria inoculation method, which comprises the following steps: (1) when tobacco seedlings grow to five leaves with a heart, the tobacco seedlings are pruned, biocontrol bacteria are inoculated to the pruned wounds of the tobacco seedlings, the inoculation is repeated three times, and a promoting agent is sprayed after each inoculation; (2) the tobacco seedlings are transplanted 7-9 days after the last pruning of the tobacco seedlings, the roots of the tobacco seedlings are dipped in the biocontrol bacteria for root dipping when the tobacco seedlings are transplanted, then the tobacco seedlings are transplanted to a field, and a promoting agent is used for root irrigation.The tobacco seedling inoculation method provided by the present application has high inoculation colonization amount of bacteria, good disease prevention and control effect of biocontrol bacteria after inoculation, can effectively prevent and control tobacco bacterial wilt, black shank disease and tobacco mosaic virus and various tobacco diseases, and has practical application value.

The invention discloses an inoculation device and an inoculation method for a tremella aurantialba liquid strain, and the inoculation method comprises the following steps: the middle end of a fungus bag is inoculated with a stereum hirsutum liquid strain, the inner surface of the fungus bag is inoculated with a tremella aurantialba liquid mixed strain, and the tremella aurantialba liquid mixed strain is a mixture of the tremella aurantialba liquid strain and the stereum hirsutum liquid strain. Preferably, the first inoculating needle is inserted into the middle end of the fungus bag to inoculate the stereum hirsutum liquid strain, and the second inoculating needle is inserted into the pit in the inner surface of the fungus bag to inoculate the tremella aurantialba liquid mixed strain. According to the method, the growth speed of tremella aurantialba hyphae is increased, the whole production period from inoculation to full fungus to fruiting is remarkably shortened, the yield is increased, and the production efficiency is improved.

The invention discloses a self-adaptive regulation and control system and a self-adaptive regulation and control method for accurate immersion inoculation of fishery vaccines. The system comprises a reaction tank, a plurality of control modules (temperature, dissolved oxygen, pH, salinity, concentration and pressure) and a controller. The controller adaptively adjusts vaccine liquid parameters based on sensor data, and the problems that an existing soaking inoculation method is unstable in immune effect and large in inoculation dosage fluctuation are solved.

The application discloses a chrysosporium strain ZJJE004 and a factory cultivation method thereof, the chrysosporium strain ZJJE004 ( Naematelia aurantialba ), is preserved in the China General Microbiological Culture Collection Center, and the preservation date is May 12, 2022, and the preservation number is CGMCC NO.40189, and the ITS sequence is shown as SEQ NO.1. The factory cultivation method of the chrysosporium strain ZJJE004 adopts a liquid strain one-time inoculation method, does not need to inoculate tissue blocks, greatly optimizes the cultivation method, and is simple in cultivation method, fast in mycelium growth, low in pollution, strong in mycelium, obviously shortens the cultivation period of the chrysosporium, and can be picked in 45-55 days; the ear yield is high, the size of the fruiting body is uniform, the mushroom effect is good, the conversion rate of the first crop can be higher than 80%, the yield is high, large-scale cultivation can be realized, has high application value, and is suitable for factory production.