484 results about "Inoculation methods" patented technology

The invention provides an artificial inoculation production method, which relates to an artificial inoculation method of Jinhua fungus of tea and a processing method of camellia nitidissima. The invention is characterized in that the artificial inoculation production method comprises the following steps of preparation of Jinhua fungus culture solution, preparation of inculating tea, artificial inoculation of Jinhua fungus, culture of Jinhua fungus, drying of products and the like. The invention has high flowering rate of the tea, uniform flowering, coarse grain of eurotium cristatum and golden color, can bring the flowering stage forward by about 3 days, can obviously improve the quality and production efficiency of the camellia nitidissima, is applicable to flowering of all teas (compressed tea and loose tea) and provides reliable guarantee for improving flowering quality of black tea.

The invention relates to the technical field of biology, in particular to a method for conducting indoor and industrialized production on Morchella esculenta. The method for conducting industrialized production on the Morchella esculenta comprises the steps of (1) cultivation mode, wherein the Morchella esculenta is suitable for being cultivated through the indoor and industrialized shelf, basin and box type; (2) raw materials, wherein sterile soil, straws, saw dust and grains are taken as raw materials; (3) formula; wherein a synthetic medium is formed according to different ratios; (4), inoculation method, wherein under the sterile condition, a first strain and a second strain are inoculated for culture; (5) bag erecting and management, wherein the industrialized production aim of the Morchella esculenta is achieved through secondary inoculation, sowing, bag erecting, manual or automatic temperature control, humidity, light and oxide control, and stimulation. After the experimental study of more than thirty years, Morchella esculenta field production is achieved, meanwhile, the worldwide technical difficult problem that in the past, the Morchella esculenta cannot be produced in an industrialized mode is solved, the method cannot be limited by areas, the Morchella esculenta can be produced throughout the year, and the economic benefit prospect and the social benefit prospect are wide.

The invention relates to a method for using fungi to elicit an Aquilariasinensis tree to produce agilawood, belonging to the biotechnological field. Two used fungi comprise a Fusaria (Fusarium sp.) fungus A-11 and a Cephalosporium (Cephalos porium sp.) fungus A-17. The content comprises the culture method and the inoculation method of the fungi. By adopting the method, the trunk of the Aquilariasinensis tree to elicited to secrete yellow brown substances similar to the chemical ingredients of agilawood. The invention has the advantages that the method is simple and convenient, the production cost is low, the period is short, the Aquilariasinensis tree resources are fully utilized and protected, and the popularization and the application are facilitated.

Disclosed are a ganoderma lucidum mycelium and a preparation method thereof. The ganoderma lucidum mycelium specifically comprises a culture medium composed of rice bran, wheat bran, corn flour and brown sugar. A ganoderma lucidum liquid strain is taken as a strain, an inoculation method and inoculation amount of the liquid strain are well grasped, and a high-quality ganoderma lucidum mycelium finished product is cultured by reasonable arrangement of the production environment. By implementation of the method, the problem that ganoderma lucidum fruiting bodies cannot be produced massively to further affect application of ganoderma lucidum medicine sources due to insufficient wild ganoderma lucidum resources and high artificial cultivation difficulty is solved. Meanwhile, the production and preparation technology of the final product, namely the ganoderma lucidum mycelium, has the advantages of short cultivation period, simplicity, low production cost, high production efficiency, high stability and the like. Besides, the final product, namely the ganoderma lucidum mycelium serving as an additive, is widely applied to the fields of pharmacy, biological health care products, special drinks, functional foods and the like, and is quite good in market prospect.

The present invention discloses an inoculation method of cistanche tubulosa by using a light medium-mesh bag container, belonging to the technical field of inoculation of parasite plant. The method comprises the steps of: bunch planting of the cistanche tubulosa seed after the inductive process of germination in the mesh bag container containing medium, cuttage with tamarix chinensis in the mesh bag container, culturing for about two months in the environment with a temperature between 25 to 35 DEG C, the air humidity larger than 70% and the water content of culture medium larger than 90%. The method can increases the root density of the tamarix chinensis and enhances the inoculation rate greatly. The invention has the advantages of short inoculation time, simple technique, low cost, small management area, labor saving, convenient transport of nursery stock, and can realize the industrialized nursery process to reduce the production cost and enhance the economic benefit.

An efficient facility earthworm breeding device is composed of a rotten reactor, an earthworm cultivation device, two earthworm breeding device bodies, an earthworm cast collecting box, an earthworm collecting box and a support. The rotten reactor is provided with a stirring device, and a material conveying device is arranged below an outlet. An earthworm cast outlet of the earthworm cultivation device is communicated with the earthworm cast collecting box. The two earthworm breeding device bodies are the same in structure, a rake-shaped scraper blade is arranged on each earthworm breeding device body so that materials can be discharged out, and the earthworm cast outlets of the earthworm breeding device bodies are communicated with the earthworm cast collecting box. The rotten reactor, the earthworm cultivation device, the two earthworm breeding device bodies and the earthworm collecting box are respectively fixedly connected with the support from top to bottom. The facility earthworm breeding device is used, a one-time earthworm inoculation method and a two-step growing method are used for conducting reducing and resource utilization of biomass. The efficient facility earthworm breeding device has the advantages that the structure is simple, operation is easy, the earthworm breeding efficiency can be effectively improved, the breeding time is shortened, the earthworm yield is improved, energy consumption is reduced, breeding cost is lowered, and safety and reliability are achieved.

The invention discloses a tobacco virus disease quick rubbing inoculation method which includes a breeding step, a preparing virus liquid step and an inoculating step. Quartz sands are evenly sprayed on true leaves of tobacco seedlings, and then a disinfection clothes brush is dipped with the virus liquid to slightly rub the tobacco leaves, and the proper result is that epidermis cells of the tobacco leaves are slightly damaged and do not die. The cloth brush is used to dip the virus liquid one time, and then rub the tobacco leaves for three times back and forth, and each time can brush for 60 cm of the tobacco leaves. materials for experiments are easy to take, and operation is simple, time-saving, labor-saving, accurate and practical. The success rate of inoculation is 100%.

An automatic microbial inoculation instrument comprises a base and an instrument rear wall. The base and the instrument rear wall are mutually perpendicular to form an instrument supporting frame, a Petri dish control mechanism (3) and a sample delivery mechanism (4) are arranged on the base, and an inoculation ring moving mechanism (1) is arranged on the rear wall of the instrument. The automatic microbial inoculation instrument has the advantages that an inoculation method, namely a multi-region marking method which is accepted by most clinical microorganism personnel, is adopted, single strains can be separated more effectively, and sample misjudgment due to technical irregularity of clinical microorganism operators can be avoided; consumable cost in use of the instrument is equivalent to the cost of manual operations, so that accuracy of clinical sample inspection is guaranteed while enthusiasm in use of the instrument in hospital departments is improved; and the automatic microbial inoculation instrument enables hospital profits to be maximized in term of economic conditions, and single strain groups can be separated more accurately to enable more accuracy in detection by the automatic microbial inoculation instrument in term of medicine.

The invention discloses a abrasive paper rubbing method of tobacco virus disease, including steps: (1) bruising leaf tissue with typical symptom of tobacco virus disease in mortar, adding water, filtering by gauze and extruding juice, then constant volume by distilled water to obtain inoculation liquor, compouded when need; (2) dividing a abrasive paper into equal portions and folding as arris; (3) dipping arris of the abrasive paper with inoculation liquor, sliding from one end to the other end of the arris on inoculation leaf, finishing inoculation. The method gets material easily, has simple, quick operation with little inaccuracy, only has three steps, success ratio of inoculation is 100%. The method suits for tobacco virus disease infected by machinery juice such as TMV, CMV, PVY, PVX and TEV and so on, especially for selecting and identifying fastness of tobacco species and experiment of prevention and cure tobacco virus disease.

The invention discloses an efficient aseptic seedling raising method of monochasma sheareri. According to the efficient aseptic seedling raising method of the monochasma sheareri, through the steps ofseed treatment, surface sterilization, inoculation, transplanting and the like, the highest seed germination rate of the monochasma sheareri reaches 100%, and the transplanting survival rate reaches86%; by adopting the inoculation method, monochasma sheareri seeds are distributed in a culture medium more uniformly, cultivated aseptic seedlings are healthy and strong and grow in order, the cleanseeds can be effectively obtained, seed dormancy is broken, and the seed surface sterilization effect is ensured, so that the seeds are dispersed on the surface of the culture medium uniformly; the operation is simple and easy, the seed utilization rate can be increased, a large number of monochasma sheareri seedlings can be obtained in a short time, and wild monochasma sheareri plant resources can be effectively protected.

The invention discloses a preparing method for clinic level cells for enhancing the immune modulating function of MSCs. The preparing method is suitable for culturing the mesenchymal stem cells from bone marrow, fat and the umbilical cord. A serum-free complete medium is adopted to culture the mesenchymal stem cells from umbilical cord tissue, a traditional method is improved by applying a secondary inoculation method, and the obtaining rate of primary cells is increased. Imidazoquinoline R848 with the concentration of 1-5 micrograms/milliliters and recombinant human interferon I FN-alpha-2b with the concentration of 100-200 units/milliliters are applied as pretreatment factors and obviously induce high expression of the MSCs and secretory cell factors VEGF, HGF and PGE-2, the capacity for modulating immune and promoting tissue regeneration of the MSCs is brought into play better, the multiplication capacity and the capacity for resisting NK cell killing of the MSCs can be improved obviously, and the pretreated MSCs have the enhanced capacity for suppressing lymphocyte proliferation and can be applied to treatment on the GVHD with T cells as the principal and other autoimmune diseases better.

The invention relates to a preparation technology for improving Fuzhuan tea pollen germination rate by using an inoculation method. The preparation technology comprises the following specific steps of: (1) purification and separation of eurotium cristatum; (2) preparation of eurotium cristatum spore suspension with certain aging degree and determination of concentration; (3) treatment on a raw dark green tea sample; (4) adding the eurotium cristatum spore suspension; (5) pressing; (6) pollen germination; and (7) drying and storage. The preparation technology develops preparation technology conditions for improving the Fuzhuan tea pollen germination rate by using the inoculation method, and can be used for realizing the pollen germination of Fuzhuan tea in a short term of 7-15 days. Moreover, golden flowers are generally blooming, the color is bright and golden, particles are plump, and the Fuzhuan tea pollen germination rate can reach 100% by using different raw dark green tea raw materials.

The invention relates to an epulorhiza sp. strain S1 (CGMCC No.8145) and application of the epulorhiza sp. strain S1 in promoting of the growth of dendrobium officinale and the accumulation of dendrobium officinale polysaccharides. According to the application, a dendrobium officinale tissue culture seedling is taken as a material and is manually inoculated with the S1 (epulorhiza sp.) strain, after the tissue culture seedling and the S1 strain are co-cultured for 60 days, S1 fungus and the root of a host form a symbiotic relationship, the fresh weight increment, dry weight, plant height, stem height, stem diameter, number of nodes, internode length, number of tillers, number of fresh roots and polysaccharide content of the tissue culture seedling are remarkably higher than a contrast, and the polysaccharide content is 141.63% higher than the content of an uncultured seedling and is about 2.4 times of the polysaccharide content of the uncultured seedling. By utilizing a novel technique, the growth of the dendrobium officinale tissue culture seedling is greatly promoted, and the biomass and the polysaccharide content of the dendrobium officinale tissue culture seedling are increased; according to a technical method, the operability is strong, the high-quality dendrobium officinale tissue culture seedling can be produced, and high economic benefits are brought to an efficient cultivation technique and industrialization production of the dendrobium officinale.

The invention provides a seeding method of Cistanche tubulosa. Chinese tamarisk ecological forest Cistanche tubulosa autoeciousness drug plants are planted in hungriness or desert of the west region, the Chinese tamarisk ecological forest is plated through wide and narrow line thick planting, drilled holes are respectively between the lines of the planted chinese tamarisk, seeds of Cistanche tubulosa is inoculated through single layer or layering insemination, or inoculation paper is input, markedly reducing working load of inoculation. After planting the Chinese tamarisk and inoculating the Chinese tamarisk with autoeciousness Cistanche tubulosa for field management, fertilization and integrated control of agricultural biology and the like, fleshy stem of Cistanche tubulosa reaches 250-550 kg yield per mu in the wide line lasted for a plurality of years, and seeds of the Cistanche tubulosa reach 1-2.2 kg yield per mu in the narrow line lasted for a plurality of years. Through detection, success ratio of inoculating the autoeciousness Cistanche tubulosa reaches 78.5-89.5%.

The invention provides a method for preparing fleckedflesh polypore mycelium. The method specifically comprises the steps that a culture medium composed of rice bran, bran, corn flour, flour and brown sugar is provided, fleckedflesh polypore liquid spawn is used as spawn, the inoculation method and the inoculation amount of the liquid spawn are well grasped, and the fleckedflesh polypore mycelium is prepared through the reasonable arrangement of a production environment. By the adoption of the method for preparing the fleckedflesh polypore mycelium, the application problems that medicinal fleckedflesh polypore resources are influenced due to the fact that wide fleckedflesh polypore resources are insufficient and a large amount of fleckedflesh polypore cannot be produced due to the large difficulty of manual cultivation are solved. The method for preparing the fleckedflesh polypore mycelium has the advantages that the cultivation period is short, the production preparation technology is simple, the production cost of the product is low, and the production efficiency of the product is high and stable. In addition, the fleckedflesh polypore mycelium as the final product is used as an additive which is widely applied to the fields of pharmacy, biological health care products, special drinks, functional foods and the like and is good in market prospect and remarkable in benefit.

The invention discloses two pairs of Ustilago esculenta for successful invasion of a zizania aquatica plant and breeding of zizania aquatica and an artificial inoculation method thereof and belongs to the technical field of zizania aquatica artificial inoculation. The two pairs of Ustilago esculenta comprise a pair of an Ustilago esculenta haploid strain UET1 and an Ustilago esculenta haploid strain UET2 and a pair of an Ustilago esculenta haploid strain UET2 and an Ustilago esculenta haploid strain UET3. Wild zizania aquatica is inoculated with the pair of the screened Ustilago esculenta haploid strain UET1 and Ustilago esculenta haploid strain UET2 and the pair of the screened Ustilago esculenta haploid strain UET2 and Ustilago esculenta haploid strain UET3 so that successful breeding of zizania aquatica is realized. The method has simple processes, is effective and provides a novel direction for breeding of zizania aquatica.

The invention relates to a method for identifying the resistance of the cytosporacarphosperma. The method evaluates pear resources and the resistance of a variety through artificial inoculation, solves the problems that high-resistance resource materials in pear disease-resistance breeding are hard to find in the early stage, and rapid evaluation is hard to achieve, and lays the foundation of breeding of the cytosporacarphosperma. The method comprises the following steps: (1) cultivating and preparing pear seedlings, (2) cultivating strains, (3) inoculating the pear seedlings, and (4) inquiring and evaluating the illness state. According to the method, the artificial inoculation technology is adopted, the good epidemic condition is provided for pathogenic bacteria, and the probability that test materials are infected by the pathogenic bacteria is ensured. By the usage of the method, identification and screening of the resistance of the cytosporacarphosperma can be rapidly and accurately carried out, and compared with the result of natural pathogenesis inquiry and identification, the result of the method is improved in accuracy and reliability.

The present invention relates to a fowlpox virus genome which has modifications in one or more wild-type FPV genes. The present invention also relates to a viral particle comprising such a genome and its use to deliver a nucleotide of interest (NOI) to a target cell. The present invention also relates to vaccination methods, particularly a method which comprises administering a priming composition (which comprises a first non-replicating viral vector) and a boosting composition (which comprises a second non-replicating viral vector) to a subject to treat and/or prevent a disease.

The invention provides a method for making hericium erinaceus fungus powder. Particularly, a culture medium composed of rice bran, bran, rice husks, corn flour and brown sugar is included. Hericium erinaceus liquid strains serve as the strains, the inoculation method and the inoculation number of the liquid strains are well mastered, and then high-quality hericium erinaceus mycelium finished produced are cultivated by reasonably arranging the production environment. By means of the method, the production and making process of the final products, namely, high-quality hericium erinaceus mycelia, has the advantages of being short in cultivation cycle, simple, low in product production cost, high in product production efficiency, stable and the like. In addition, by means of the method, the final products, namely, high-quality hericium erinaceus mycelia, are widely applied to the fields such as the pharmacy field, the biological health care product field, the special drink field and the functional food field as an additive.

The invention belongs to the technical field of plant protection, and particularly relates to an efficient stable indoor inoculation method for Ustilaginoidea virens and a special strain. The method includes: subjecting a hyphal mass (6mm) cultivated for 7 days on PSA (potato dextrose agar) to shake cultivation through PSB (photosynthetic bacteria) for 7-10 days so as to prepare hypha and spore mixed liquor, and filtering the mixed liquor with four layers of filter cloth to obtain spore liquor inoculants 106/ml; inoculating rice ear bracts by injecting at the seventh stage of rice young ear differentiation; and keeping moist for three days at 25 DEG C and under 95%RH (relative humidity), and placing in a spray irrigation net chamber for cultivation at 25-32 DEG C and under 90%-98%RH. By the method, the rate of diseased ears under artificial inoculation of Ustilaginoidea virens can reach more than 90%. By the method compared with the outdoor artificial inoculation techniques, efficientstable incidence of Ustilaginoidea virens can be obtained under controllable conditions.

The invention discloses a method for producing preserved beancurd with bean dregs as raw materials. The method comprises the following steps: (1) squeezing the bean dregs, cutting the bean dregs into chunks, sterilizing the chunks under high pressure and cooling the chunks to 27-32 DEG C to prepare bean dreg billets; (2) inoculating mucor undergoing amplification culture to the six surfaces of the bean dreg billets by a spraying inoculation method and carrying out fermentation and roughing; (3) carrying out salting; and (4) inoculating aspergillus oryzae and saccharomyces cerevisiae, thus preparing the preserved beancurd. The method has the following beneficial effects: the bean dregs, wastes from bean product processing, are taken as the raw materials, thus turning waste into wealth, solving the problem of pollution caused by bean product production and improving the added value of bean product processing; the industrial chain of bean product processing is lengthened; the used raw materials are cheap and easy to obtain, thus lowering the production cost of the preserved beancurd; the product has good taste and unique flavor; the sensory indexes of the product are close to the sensory indexes of sauce preserved beancurd; and the preserved beancurd is brown, has a little gloss, neat and uniform chunks and fine texture, is free from mould core and impurities, is tasty, has brackish palatability and is free from extraneous odor.

The invention discloses a quick, simple and effective tomato yellow leaf curl virus resistance determination method and belongs to the field of plant protection. Traditionally, the determination of the tomato yellow leaf curl virus resistance is performed by a Bemisia tabaci (Gennadius) inoculation determination method, while the Bemisia tabaci (Gennadius) inoculation determination method has difficulty in providing uniform and consistent inoculation pressure due to the influences (such as the number of the inoculated Bemisia tabaci (Gennadius), virus rate and feeding habit) of the Bemisia tabaci (Gennadius) and at the same time, suffers interference from other Bemisia tabaci (Gennadius) transmitted viruses, so the accuracy of the determination result is low. The agrobacterium rhizogenes injection inoculation method well solves the problem. The resistance of a tomato material to the tomato yellow leaf curl viruses can be determined by using constructed infectious clones and directly inoculating the tomato yellow leaf curl viruses by injection in stalks. The method avoids culturing Bemisia tabaci (Gennadius), ensures simple operation, high efficiency, high repeatability and high controllability, provides a relatively consistent pressure, obtains accurate result and is a simple and effective inoculation determination method.