44 results about "Inoculation methods" patented technology

This invention discloses an image recognition-based method for assessing grape powdery mildew. By introducing standardized sample preparation and uniform inoculation methods, this invention reduces the interference of randomness and spatial heterogeneity of natural field infection on the assessment results, making the disease occurrence process more controllable among different materials, thereby improving the stability and comparability of phenotypic data. Simultaneously, image recognition and machine learning technologies are used to automatically identify and calculate the area of ​​powdery mildew-infected regions, avoiding the reliance on the experience of assessors in traditional manual visual grading. This enables a continuous quantitative description of the degree of powdery mildew infection, improving the consistency and accuracy of the assessment results. This invention constructs a large-scale image analysis workflow that can be used for batch processing, significantly improving the efficiency of disease assessment, reducing the workload of manual investigation, and making this method applicable to applications requiring the processing of large numbers of samples, such as germplasm resource evaluation and breeding material screening.

The invention discloses a Ustilaginoidea virens inoculant, a preparation method and application of the Ustilaginoidea virens inoculant in Ustilaginoidea virens inoculation, and belongs to the technical field of agricultural microorganisms. According to the method, two-step shake-flask culture is combined with the steps of filtering, centrifuging and spore germination, so that the ustilaginoidea virens inoculum taking germinated spores (GC) as the core is prepared, and the problems of hypha aging, low spore activity, asynchronous infection and the like in the existing inoculation method are effectively solved. The inoculant has a tender hypha structure and strong infection activity, and the inoculation efficiency and the pathogenesis consistency are remarkably improved. When being used for preparing transcriptome samples, metabolome samples and other omics samples, the strain avoids non-specific immune interference caused by PAMPs released by broken hyphae, ensures synchronous infection process, and is real and reliable in immune response. The invention provides a stable and efficient inoculation system with a clean background for the research on the ustilaginoidea virens-rice interaction mechanism, and has a good application prospect.

The invention provides a spore solid bacterium inoculation method. Relates to the technical field of edible mushrooms. The method comprises the following steps: S1, culture medium group preparation: selecting trees and setting a spore source culture medium group and a receptor culture medium group in groups; s2, inducing the spore source culture medium group to form a mature sporocarp, inoculating a target edible mushroom strain on the spore source culture medium group, inducing the strain to grow and forming the mature sporocarp; s3, controlling environmental parameters to promote spores to eject and fall on the receptor culture medium group; s4, the sporocarp is obtained through natural culture, the spore source culture medium group accounts for 5%-20% of the total culture medium group in volume, the surface of the receptor culture medium group in the S1 needs to be subjected to scribing treatment, the depth of a notch is 2-5 mm, the condition that the notch makes contact with the xylem is adopted as the criterion, and if the length of the tilia wood is smaller than 30 cm, scribing is not needed. The spore solid bacterium inoculation method provided by the invention has the advantages of reducing the planting cost and improving the economic benefit.

The invention relates to an automatic inoculation method and device for a pouring method in microbiological counting, and belongs to the field of microbiological devices.The automatic inoculation method comprises the following steps that a plurality of culture dishes added with sample liquid are placed in a lower dish channel and transferred to a dish receiving station; in the dish receiving station, the bottoms of the culture dishes are moved downwards to a filling disc through a guide cylinder material plate I, and the filling disc transfers the culture dishes to the inoculation station; in the inoculation station, a culture medium is injected into the culture dish bottom, after a filling disc swings and shakes clockwise and anticlockwise in a reciprocating mode, a control wheel drives the culture dish bottom to rotate, and the culture dish bottom enters a transition station after rotating; the transition station is used for transferring the culture dishes into the code printing station; the bottom of the culture dish is coded and transferred to the stacking station; after the bottoms of the culture dishes are supported and buckled with the culture dish covers, the culture dishes are dragged into the stacking channels, and through the steps, dish receiving, inoculation, transition, code printing and stacking of the culture dishes in the multiple dish discharging channels are completed. Human factor interference in the inoculation process is reduced to the greatest extent, and the inoculation process of a pouring method can be efficiently completed.

The invention discloses a novel indoor identification method for the ringspot virus resistance of a new transgenic papaya variety, and belongs to the technical field of plant virus inoculation. According to the method, a sterile steel nail brush is used for dipping inoculation liquid containing ringspot viruses, indoor cultivated papaya leaves are inoculated, cultivation is conducted for 10 days after inoculation is completed, disease investigation is conducted on transgenic papaya, and the transgenic papaya is obtained according to the disease index of the inoculated transgenic papaya. And identifying the resistance of the transgenic papaya to the ringspot virus. The inoculation method adopted by the invention is simple and convenient to operate, has the advantages of high inoculation efficiency and good repeatability, can realize rapid batch inoculation of the ringspot virus on the papaya, and is not only suitable for screening new varieties of transgenic papaya resistant to ringspot, but also suitable for screening new varieties of transgenic papaya resistant to ringspot. And the method also has important significance for exploring the pathogenic mechanism of the ringspot virus and the disease-resistant mechanism of disease-resistant papaya varieties.

Vaccination methods include applying to a subject a microneedle patch having microneedles including a vaccine formulation including one or more infectious disease antigens. Infant and child subjects were found to tolerate application of the patch for a period effective to administer the antigen. The patch may be applied to the skin of a subject for an application period of five minutes or less, and effective to administer the antigen to the subject, wherein at least 50% of the height of the microneedle dissolves into the skin of the subject during the application period. Thermally stable vaccine formulations for the microneedles are provided. The methods may be effective to protect the subject from the infectious disease even if, prior to the applying step, the drug delivery device is transported or stored out of cold chain conditions for up to 14 days.

The utility model discloses a microorganism inoculation device applied to plant leaves, which comprises a plurality of fitting protection pieces, round bulges are arranged in the fitting protection pieces, accommodating cavities are formed in the bottoms of the round bulges, and disinfection cotton balls and target fungus cakes are sequentially arranged in the accommodating cavities from top to bottom. And two mounting grooves are formed in the bottom of each attaching protection piece, and high-temperature-resistant adhesives are arranged in the two mounting grooves. The utility model mainly has the following advantages: 1, the requirement on the inoculation environment is not too high, and the inoculation can be carried out under the conditions of high temperature and high humidity; 2, the inoculation method realizes the effects of wind resistance, high temperature resistance and rain resistance; 3, one leaf can realize inoculation of different strains or a plurality of fungus cakes of the same strain; 4, only the positions for inoculating the fungus cakes need to be covered with plastic, so that the fungus cakes are light-permeable and breathable, and field planting and disease attack are facilitated; and 5, the inoculation method is simple, and a plurality of operation steps can be completed indoors.

The invention relates to the technical field of plasmodiophora brassicae inoculation, in particular to an artificial inoculation method for plasmodiophora brassicae immediate infection and application, and the method specifically comprises the following steps: S1, accelerating germination: accelerating germination of seeds to obtain seedlings; s2, grinding bacteria and preparing a bacteria solution; s3, inoculation: performing field planting on the seedlings obtained in the step S1, and then performing injection inoculation on the overground parts of the seedlings by using the bacterial liquid prepared in the step S2; s4, seedling stage management; s5, disease investigation. According to the method, the cognitive limitation of traditional two-step infection of root hair infection and cortex diffusion is broken through, a brand-new inoculation technical path is constructed, and a new idea and method for inoculating plasmodiophora brassicae of cruciferae are developed; the defects that a traditional underground inoculation method is fuzzy in infection time sequence, uncontrollable in inoculation metering, low in inoculation efficiency and poor in pathogenesis stability due to large bacterial quantity fluctuation are fundamentally overcome.

This invention provides an inoculation device and method for edible fungi spawn, belonging to the technical field of edible fungi production equipment. The inoculation device and method include a frame and a conveyor. The conveyor transports the spawn. A pressing plate is slidably mounted on the frame, and the pressing plate has inoculation holes corresponding to the positions of the spawn. An elevator is mounted on the frame, and a receiving plate is installed at the bottom of the elevator. A needle corresponding to the position of the inoculation hole is located at the bottom of the receiving plate. Inside the pressing plate is a cleaning mechanism consisting of a toothed ring, brush bristles, transmission gears, drive gears, a drive sleeve, and a threaded rod. The linear motion of the receiving plate's lifting and lowering is converted into the rotational motion of the toothed ring, automatically brushing and cleaning the outer wall of the needle during needle piercing and retraction. This effectively removes residual culture medium, avoids needle clogging and cross-contamination, eliminates the need for manual cleaning during machine downtime, and significantly improves continuous operation time and inoculation quality.

The invention relates to a method for rapidly and stably identifying rice blast resistance indoors and application, and belongs to the technical field of plant disease prevention and control. The invention provides a plum culture medium which takes water as a solvent and comprises 30-60 mL / L of prune juice, 3-10 g / L of alpha-lactose, 0.5-2 g / L of yeast extract and 10-20 g / L of agar powder. The pH value of the plum culture medium is 6.5 to 7.5. According to the inoculation method disclosed by the invention, the inoculation success rate and the scab appearing degree of the pyricularia oryzae are remarkably improved by optimizing the key steps of seed soaking and germination acceleration, strain activation and propagation, conidium suspension preparation, spray inoculation and the like, and the inoculation method has the advantages of low cost, simplicity and convenience in operation, high standardization degree, good inoculation effect, small batch difference and high repeatability.

The invention belongs to the technical field of plant gene function research, and particularly relates to a method for inducing endogenous gene silencing of common tobacco (Nicotiana tabacum) by using a tobacco embrittlement virus. In order to solve the problems of unstable silencing efficiency, poor systematic silencing effect and the like of the existing TRV-VIGS technology in common tobacco, various key parameters about tobacco varieties, virus inoculation methods and plant culture conditions in the implementation process of the TRV-VIGS technology are determined, and the problems of common tobacco are effectively solved by adopting the method provided by the invention. The endogenous NtPDS gene of common tobacco is successfully silenced, and the silencing efficiency is as high as 99.02%.

The invention discloses an in-vitro high-throughput inoculation method of tea tree colletotrichum gloeosporioides, and belongs to the technical field of tea tree biology. The method comprises the following steps: preparing a spore suspension with the concentration gradient (absorbance) OD600 of 0.4, 0.6, 0.8 and 1.0 from the Colletotrichum calamiae, inoculating the spore suspension to an in-vitro tea tree leaf in an injection manner, then placing the leaf in a high-throughput microenvironment culture device, and culturing for 4 days under the conditions of 28 DEG C, 75% relative humidity and 12h illumination / 12h darkness, thereby accurately evaluating the infection efficiency of the pathogenic bacteria. According to the method, a standardized inoculation process is combined with high-throughput microenvironment control, so that the problems of long period, non-uniform environment and low efficiency of a traditional method are solved, and the method has the outstanding advantages of short inoculation period, good experimental result consistency and capability of realizing batch high-throughput screening; the method can be directly used for tea tree colletotrichum gloeosporioides resistance identification, pathogen toxicity analysis and bactericide screening.

The invention belongs to the field of plant disease infection, and discloses a quantitative inoculation method for infecting fungal diseases from plant wounds and application of the quantitative inoculation method. The quantitative inoculation method for infecting fungal diseases from plant wounds comprises the following steps: S1, formation of plant micro wounds: spraying quartz sand to host plant tissues by adopting a quartz sand spray gun, forming a plurality of micro wounds on the surfaces of the host plant tissues, and shaking off quartz sand grains on the plant tissues; and S2, spraying the fungal spore suspension to the leaf surface of the injured host plant, sealing the inoculated plant tissue, culturing to keep a water film on the surface of the inoculated leaf, and culturing until the plant tissue is successfully infected by fungi. The quantitative inoculation method can be applied to plant fungal disease research and plant fungal disease control method formulation. According to the quantitative inoculation method, the number of the micro-wounds formed on the surface of the plant tissue is controllable, counting is convenient, repeatability is good, and applicability is high.

The invention belongs to the field of crop disease resistance identification. The invention discloses a method for accurately evaluating the resistance of a corn variety to fusarium verticillium ear rot. The method comprises the following steps: inoculating corn ears by adopting a needling inoculation method, harvesting, threshing and grinding the ears in a harvesting period, measuring the content of fumonisins in grains, and evaluating the resistance of a corn variety to fusarium verticillium ear rot through the content of fumonisins in the grains. According to the method, the resistance of the corn variety to the fusarium verticillium ear rot can be evaluated more accurately, and the corn variety harmful to human and livestock is eliminated, so that the food safety is guaranteed.

The invention relates to a greenhouse fungus soil inoculation method for fusarium graminearum stem rot of corn, which comprises the following steps: activating fusarium graminearum strains, cutting hypha blocks at the edges of bacterial colonies, and inoculating the hypha blocks into a mung bean soup culture medium to prepare fusarium graminearum spore suspension; inoculating the spore suspension to sterilized corn kernels to prepare fusarium graminearum corn kernel inoculums; preparing bacterial soil, mixing bacteria and sowing in a greenhouse, performing disease grade investigation and resistance evaluation after 20 days, calculating the average disease grade of all plants of the to-be-identified corn material, and determining the resistance of the to-be-identified corn material to the fusarium graminearum stem rot. The inoculation method does not depend on a field environment, is short in inoculation period, high in efficiency and stable in result, can be combined with a field inoculation test to assist in verifying the resistance of a corn material to stem rot, and can be used for identifying and evaluating the disease resistance of a corn hybrid or a selfing line material.

The invention relates to the technical field of plant and microorganism interaction. The invention provides a method for inoculating camellia oleifera seedlings by using endophytic fungus piriformospora indica, which comprises the following steps of: inoculating an endophytic fungus piriformospora indica spore suspension into camellia oleifera seedling culture substrates of different depth layers in 2-3 times, keeping the substrates wet, and regularly weeding and preventing and treating diseases and insect pests. The invention also provides application of the endophytic fungus piriformospora indica in promoting the growth of camellia oleifera, improving the quality of camellia oleifera and improving the physicochemical properties of camellia oleifera soil. The invention further provides a layered inoculation method which can improve the colonization rate and growth promoting effect of oil tea root systems and remarkably improve the growth performance of oil tea seedlings. The colonization rate and the growth promoting effect are kept stable in different environments, and the method is suitable for large-scale field popularization.

The invention provides a soybean anthracnose inoculation method and application. The soybean anthracnose inoculation method comprises the steps of soybean colletotrichum gloeosporioides culture, soybean sprout culture and soybean colletotrichum gloeosporioides living body inoculation. The method comprises the following steps: cultivating soybean sprouts, cutting off roots of the soybean sprouts, inoculating stems of the soybean sprouts, putting the stems into a cryopreservation tube filled with wet degreasing cotton balls after inoculation, culturing at the temperature of 25 DEG C in darkness for 3 days, and counting morbidity according to established disease grading standards. The soybean anthracnose inoculation method provided by the invention has the characteristics of short period, simple and efficient operation steps, high accuracy and strong applicability, and provides technical support for soybean variety anthracnose resistance identification and in-vivo inoculation determination of the control effect of a bactericide.

The invention discloses a fishing vaccine continuous soaking inoculation method based on the Archimedes screw principle. The fishing vaccine continuous soaking inoculation method comprises the steps that fish schools and vaccine soaking liquid are synchronously conveyed to a cavity formed between a soaking cylinder and an oxygen pumping shaft pipe through a sliding way; the oxygen pumping shaft pipe and the soaking cylinder are divided by the spiral blades to form independent cavities, so that fish schools with proper density are continuously propelled and uniformly soaked in compartments according to a set propelling speed; the oxygen dissolving level of the vaccine soaking solution is dynamically maintained through the oxygen pumping shaft tube; the fish school state is monitored in real time based on an image acquisition device, and abnormal individuals are marked; the vaccine soak solution is recycled through the separation grid plate and the recovery system. The problems that traditional artificial inoculation is large in batch difference, low in inoculation efficiency, unstable in dissolved oxygen and large in fish school stress are solved.

The invention discloses a negative pressure fixation and micro-injection combined cotton virus-induced gene silencing inoculation method. The method comprises the following steps: (1) constructing a virus-induced gene silencing vector; (2) preparing an agrobacterium suspension; (3) fixing under negative pressure; (4) performing micro-injection inoculation; and (5) subsequent culture: culturing the inoculated plant under the conditions that the temperature is 22-25 DEG C, the illumination period is 16h illumination / 8h darkness, the illumination intensity is 100-200 and the relative humidity is 60-80%, and observing the gene silencing phenotype after 7-14 days. The method solves the problems that a traditional virus-induced gene silencing inoculation method is complex in operation, low in success rate and large in leaf damage, has the advantages of being easy and convenient to operate, high in inoculation success rate, small in leaf damage, high in gene silencing efficiency and the like, and is suitable for cotton functional genomics research and gene function verification.

The present application relates to the technical field of tobacco biocontrol bacteria inoculation, and particularly relates to a tobacco biocontrol bacteria inoculation method.The present application provides a tobacco biocontrol bacteria inoculation method, which comprises the following steps: (1) when tobacco seedlings grow to five leaves with a heart, the tobacco seedlings are pruned, biocontrol bacteria are inoculated to the pruned wounds of the tobacco seedlings, the inoculation is repeated three times, and a promoting agent is sprayed after each inoculation; (2) the tobacco seedlings are transplanted 7-9 days after the last pruning of the tobacco seedlings, the roots of the tobacco seedlings are dipped in the biocontrol bacteria for root dipping when the tobacco seedlings are transplanted, then the tobacco seedlings are transplanted to a field, and a promoting agent is used for root irrigation.The tobacco seedling inoculation method provided by the present application has high inoculation colonization amount of bacteria, good disease prevention and control effect of biocontrol bacteria after inoculation, can effectively prevent and control tobacco bacterial wilt, black shank disease and tobacco mosaic virus and various tobacco diseases, and has practical application value.

The invention discloses an inoculation device and an inoculation method for a tremella aurantialba liquid strain, and the inoculation method comprises the following steps: the middle end of a fungus bag is inoculated with a stereum hirsutum liquid strain, the inner surface of the fungus bag is inoculated with a tremella aurantialba liquid mixed strain, and the tremella aurantialba liquid mixed strain is a mixture of the tremella aurantialba liquid strain and the stereum hirsutum liquid strain. Preferably, the first inoculating needle is inserted into the middle end of the fungus bag to inoculate the stereum hirsutum liquid strain, and the second inoculating needle is inserted into the pit in the inner surface of the fungus bag to inoculate the tremella aurantialba liquid mixed strain. According to the method, the growth speed of tremella aurantialba hyphae is increased, the whole production period from inoculation to full fungus to fruiting is remarkably shortened, the yield is increased, and the production efficiency is improved.

The invention discloses a self-adaptive regulation and control system and a self-adaptive regulation and control method for accurate immersion inoculation of fishery vaccines. The system comprises a reaction tank, a plurality of control modules (temperature, dissolved oxygen, pH, salinity, concentration and pressure) and a controller. The controller adaptively adjusts vaccine liquid parameters based on sensor data, and the problems that an existing soaking inoculation method is unstable in immune effect and large in inoculation dosage fluctuation are solved.

The application discloses a chrysosporium strain ZJJE004 and a factory cultivation method thereof, the chrysosporium strain ZJJE004 ( Naematelia aurantialba ), is preserved in the China General Microbiological Culture Collection Center, and the preservation date is May 12, 2022, and the preservation number is CGMCC NO.40189, and the ITS sequence is shown as SEQ NO.1. The factory cultivation method of the chrysosporium strain ZJJE004 adopts a liquid strain one-time inoculation method, does not need to inoculate tissue blocks, greatly optimizes the cultivation method, and is simple in cultivation method, fast in mycelium growth, low in pollution, strong in mycelium, obviously shortens the cultivation period of the chrysosporium, and can be picked in 45-55 days; the ear yield is high, the size of the fruiting body is uniform, the mushroom effect is good, the conversion rate of the first crop can be higher than 80%, the yield is high, large-scale cultivation can be realized, has high application value, and is suitable for factory production.

The invention provides a rapid breeding method for rust-resistant oats. The method comprises the steps of rust-resistant parent material selection and hybridization, F1-generation population seed treatment, F2-generation population resistance identification and single plant selection, F3-F8-generation population screening, molecular marker-assisted selection and homozygous strain creation, and strain field identification and variety breeding. The invention aims to solve the technical problems that the anti-rust breeding of oat is difficult to combine with'temperature and light regulation and fertility stage suitability ', and the breeding period is too long. By controlling the temperature and red and blue light in a plant growth process in a staged precise control manner, the orientated shortening of the oat growth cycle, the improvement of the light energy utilization efficiency and the enhancement of the environmental stability in a key growth stage are realized, and a specific rust disease inoculation method is combined, so that the rust disease resistance phenotype can be stably expressed, and the precise screening of offspring is facilitated. By means of breeding screening of multiple breeding generations in the technology, an efficient and controllable rapid breeding system can be established, and rapid breeding of disease-resistant high-yield varieties is achieved.

This invention belongs to the technical field of bacterial culture transfer equipment, and more specifically, relates to an imprinted bacterial culture dot matrix inoculation block, device, and method. The invention includes a thin-film inoculation block body, which is divided into a smooth pickup section and an imprinted inoculation section. The imprinted inoculation section includes at least one pressure relief groove and dot matrix protrusions located on both sides of the pressure relief groove. Each dot matrix protrusion includes several raised portions, which are symmetrically and evenly distributed in a matrix on both sides of the pressure relief groove. Each raised portion has a concentric recess at its top center. This invention, through its thin-film inoculation block body, through-type pressure relief groove, matrix dot matrix protrusions, and concentric recess structure, combined with a porous liquid storage pad for quantitative liquid supply and an automated pickup and imprinting mechanism, solves the problems of poor consistency, vacuum adhesion, cross-contamination, low automation, and lack of process traceability in existing inoculation methods, achieving high-throughput, standardized, pollution-free, and traceable bacterial culture dot matrix inoculation.

The invention relates to the technical field of biological experiment equipment, and provides an automatic cell inoculation operation device and a liquid separation method for cell inoculation, and the automatic cell inoculation operation device comprises a liquid separation module, an inoculation needle assembly, a vibration module and a positioning module. A liquid storage cavity, a flow dividing pipe, a buffering groove, a thin liquid cavity and a liquid dividing cavity are sequentially formed in the liquid dividing module from top to bottom. The inoculating needle assembly is located above the liquid storage cavity and used for injecting cell suspension into the liquid storage cavity. The inlet of the overflow channel is higher than the highest liquid level of the buffer tank, the tail end of the overflow channel is connected to the bottom of the liquid storage cavity, and the highest position of the overflow channel is located above the liquid level of the liquid storage cavity. A liquid outlet channel is arranged below the liquid separation cavity, the liquid outlet channel is in one-to-one correspondence with the culture holes of the culture dish, the vibration module comprises a vibration pad, and the control system is electrically connected with the inoculation needle assembly, the liquid separation module, the vibration module and the positioning module. Cells are prevented from being accumulated in the center of the hole bottom due to the inertia effect, and the cells are evenly distributed in the hole.

The invention relates to a method for evaluating the soft rot resistance level of aseptically cultured colored callalily. The method comprises the following steps: preparing in-vitro leaves of aseptically cultured colored callalily to be detected; placing the in-vitro leaves on a moisturizing device for moisturizing culture; preparing a bacterial liquid of the Erwinia solani, wherein the concentration of the bacterial liquid is lower than or equal to the quorum sensing threshold concentration of the Erwinia solani; inoculating a predetermined volume of the bacterial liquid at a petiole incision of the in-vitro leaf; at different time points after inoculation, the morbidity condition of the in-vitro leaves is observed and quantified; and evaluating the soft rot resistance level of the aseptically cultured colored callalily based on the quantitative result of the morbidity condition. On the basis of sterile and rapid propagation of the zantedeschia aethiopica, the inoculation method has the characteristics of simplicity, convenience, high efficiency and easiness in popularization, is of great significance to development of disease-resistant breeding of the zantedeschia aethiopica, and provides a new technology and method for disease-resistant breeding of other plants, plant-microorganism interaction and research in related fields of biotechnology.

Methods of vaccination utilizing a cationic liposomal adjuvant admixed with at least one neoepitope and a solvent. Also provided are unit dosages and compositions for use in the methods.

The application discloses an intraoperative tissue specimen anaerobic culture bottle and a seeding method thereof, and aims at solving the problems of complicated early processing steps, time waste and specimen pollution during processing in the prior art, which affects the authenticity of results. The application comprises a bottle body, an accommodation cavity is arranged on the upper part of the bottle body along the height direction, and a culture cavity is arranged on the lower part of the bottle body along the height direction; an inner cover is arranged at the joint of the accommodation cavity and the culture cavity, and a cylindrical outer cover is arranged in the accommodation cavity; during use, the inner cover is closed, the outer cover is used to grind the tissue specimen, the inner cover is opened after grinding, the slurry culture solution falls into the culture cavity, the inner cover is closed, and sealed culture is carried out; the application has the advantages of simple structure and operation steps, convenient use, time saving, direct specimen processing and inoculation after the specimen is taken out during operation, less pollution of the tissue specimen, improved inoculation positive rate of the tissue specimen, and improved authenticity of results.

The invention discloses brachybacterium paragglutinum and application of the brachybacterium paragglutinum to improvement of heat resistance of coral adults, the brachybacterium paragglutinum WH11-3 is preserved in Guangdong Microbial Culture Collection Center, and a method for improving the heat resistance of the coral adults by using the brachybacterium paragglutinum WH11-3 comprises the following steps: collecting the coral adults from seawater, and domesticating the coral adults in a water body; resuspending the pair of brevibacterium agglomerans into sterilized seawater to prepare a bacterial suspension; adding the bacterial suspension into a water body to infect coral adults, and transferring the coral adults into a fresh water body for continuous culture to obtain the coral adults infected with the brachybacterium paragasteroides. The photosynthetic efficiency of the coral adult in a high-temperature environment can be remarkably improved in a short time by inoculating the brachybacterium paragasteroides to the coral adult, the method can be popularized and applied to other coral adults except for the antler cup-shaped coral adult, and the inoculation method is easy to operate, low in cost, safe and free of pollution. The coral adult heat resistance is improved, so that the coral reef ecological system is assisted to be restored and protected.