26 results about "Inoculation methods" patented technology

This invention discloses an image recognition-based method for assessing grape powdery mildew. By introducing standardized sample preparation and uniform inoculation methods, this invention reduces the interference of randomness and spatial heterogeneity of natural field infection on the assessment results, making the disease occurrence process more controllable among different materials, thereby improving the stability and comparability of phenotypic data. Simultaneously, image recognition and machine learning technologies are used to automatically identify and calculate the area of ​​powdery mildew-infected regions, avoiding the reliance on the experience of assessors in traditional manual visual grading. This enables a continuous quantitative description of the degree of powdery mildew infection, improving the consistency and accuracy of the assessment results. This invention constructs a large-scale image analysis workflow that can be used for batch processing, significantly improving the efficiency of disease assessment, reducing the workload of manual investigation, and making this method applicable to applications requiring the processing of large numbers of samples, such as germplasm resource evaluation and breeding material screening.

The invention discloses a Ustilaginoidea virens inoculant, a preparation method and application of the Ustilaginoidea virens inoculant in Ustilaginoidea virens inoculation, and belongs to the technical field of agricultural microorganisms. According to the method, two-step shake-flask culture is combined with the steps of filtering, centrifuging and spore germination, so that the ustilaginoidea virens inoculum taking germinated spores (GC) as the core is prepared, and the problems of hypha aging, low spore activity, asynchronous infection and the like in the existing inoculation method are effectively solved. The inoculant has a tender hypha structure and strong infection activity, and the inoculation efficiency and the pathogenesis consistency are remarkably improved. When being used for preparing transcriptome samples, metabolome samples and other omics samples, the strain avoids non-specific immune interference caused by PAMPs released by broken hyphae, ensures synchronous infection process, and is real and reliable in immune response. The invention provides a stable and efficient inoculation system with a clean background for the research on the ustilaginoidea virens-rice interaction mechanism, and has a good application prospect.

The invention provides a spore solid bacterium inoculation method. Relates to the technical field of edible mushrooms. The method comprises the following steps: S1, culture medium group preparation: selecting trees and setting a spore source culture medium group and a receptor culture medium group in groups; s2, inducing the spore source culture medium group to form a mature sporocarp, inoculating a target edible mushroom strain on the spore source culture medium group, inducing the strain to grow and forming the mature sporocarp; s3, controlling environmental parameters to promote spores to eject and fall on the receptor culture medium group; s4, the sporocarp is obtained through natural culture, the spore source culture medium group accounts for 5%-20% of the total culture medium group in volume, the surface of the receptor culture medium group in the S1 needs to be subjected to scribing treatment, the depth of a notch is 2-5 mm, the condition that the notch makes contact with the xylem is adopted as the criterion, and if the length of the tilia wood is smaller than 30 cm, scribing is not needed. The spore solid bacterium inoculation method provided by the invention has the advantages of reducing the planting cost and improving the economic benefit.

The invention relates to the technical field of plasmodiophora brassicae inoculation, in particular to an artificial inoculation method for plasmodiophora brassicae immediate infection and application, and the method specifically comprises the following steps: S1, accelerating germination: accelerating germination of seeds to obtain seedlings; s2, grinding bacteria and preparing a bacteria solution; s3, inoculation: performing field planting on the seedlings obtained in the step S1, and then performing injection inoculation on the overground parts of the seedlings by using the bacterial liquid prepared in the step S2; s4, seedling stage management; s5, disease investigation. According to the method, the cognitive limitation of traditional two-step infection of root hair infection and cortex diffusion is broken through, a brand-new inoculation technical path is constructed, and a new idea and method for inoculating plasmodiophora brassicae of cruciferae are developed; the defects that a traditional underground inoculation method is fuzzy in infection time sequence, uncontrollable in inoculation metering, low in inoculation efficiency and poor in pathogenesis stability due to large bacterial quantity fluctuation are fundamentally overcome.

This invention provides an inoculation device and method for edible fungi spawn, belonging to the technical field of edible fungi production equipment. The inoculation device and method include a frame and a conveyor. The conveyor transports the spawn. A pressing plate is slidably mounted on the frame, and the pressing plate has inoculation holes corresponding to the positions of the spawn. An elevator is mounted on the frame, and a receiving plate is installed at the bottom of the elevator. A needle corresponding to the position of the inoculation hole is located at the bottom of the receiving plate. Inside the pressing plate is a cleaning mechanism consisting of a toothed ring, brush bristles, transmission gears, drive gears, a drive sleeve, and a threaded rod. The linear motion of the receiving plate's lifting and lowering is converted into the rotational motion of the toothed ring, automatically brushing and cleaning the outer wall of the needle during needle piercing and retraction. This effectively removes residual culture medium, avoids needle clogging and cross-contamination, eliminates the need for manual cleaning during machine downtime, and significantly improves continuous operation time and inoculation quality.

The invention belongs to the technical field of plant gene function research, and particularly relates to a method for inducing endogenous gene silencing of common tobacco (Nicotiana tabacum) by using a tobacco embrittlement virus. In order to solve the problems of unstable silencing efficiency, poor systematic silencing effect and the like of the existing TRV-VIGS technology in common tobacco, various key parameters about tobacco varieties, virus inoculation methods and plant culture conditions in the implementation process of the TRV-VIGS technology are determined, and the problems of common tobacco are effectively solved by adopting the method provided by the invention. The endogenous NtPDS gene of common tobacco is successfully silenced, and the silencing efficiency is as high as 99.02%.

The invention discloses an in-vitro high-throughput inoculation method of tea tree colletotrichum gloeosporioides, and belongs to the technical field of tea tree biology. The method comprises the following steps: preparing a spore suspension with the concentration gradient (absorbance) OD600 of 0.4, 0.6, 0.8 and 1.0 from the Colletotrichum calamiae, inoculating the spore suspension to an in-vitro tea tree leaf in an injection manner, then placing the leaf in a high-throughput microenvironment culture device, and culturing for 4 days under the conditions of 28 DEG C, 75% relative humidity and 12h illumination / 12h darkness, thereby accurately evaluating the infection efficiency of the pathogenic bacteria. According to the method, a standardized inoculation process is combined with high-throughput microenvironment control, so that the problems of long period, non-uniform environment and low efficiency of a traditional method are solved, and the method has the outstanding advantages of short inoculation period, good experimental result consistency and capability of realizing batch high-throughput screening; the method can be directly used for tea tree colletotrichum gloeosporioides resistance identification, pathogen toxicity analysis and bactericide screening.

The invention relates to the technical field of plant and microorganism interaction. The invention provides a method for inoculating camellia oleifera seedlings by using endophytic fungus piriformospora indica, which comprises the following steps of: inoculating an endophytic fungus piriformospora indica spore suspension into camellia oleifera seedling culture substrates of different depth layers in 2-3 times, keeping the substrates wet, and regularly weeding and preventing and treating diseases and insect pests. The invention also provides application of the endophytic fungus piriformospora indica in promoting the growth of camellia oleifera, improving the quality of camellia oleifera and improving the physicochemical properties of camellia oleifera soil. The invention further provides a layered inoculation method which can improve the colonization rate and growth promoting effect of oil tea root systems and remarkably improve the growth performance of oil tea seedlings. The colonization rate and the growth promoting effect are kept stable in different environments, and the method is suitable for large-scale field popularization.

The invention provides a soybean anthracnose inoculation method and application. The soybean anthracnose inoculation method comprises the steps of soybean colletotrichum gloeosporioides culture, soybean sprout culture and soybean colletotrichum gloeosporioides living body inoculation. The method comprises the following steps: cultivating soybean sprouts, cutting off roots of the soybean sprouts, inoculating stems of the soybean sprouts, putting the stems into a cryopreservation tube filled with wet degreasing cotton balls after inoculation, culturing at the temperature of 25 DEG C in darkness for 3 days, and counting morbidity according to established disease grading standards. The soybean anthracnose inoculation method provided by the invention has the characteristics of short period, simple and efficient operation steps, high accuracy and strong applicability, and provides technical support for soybean variety anthracnose resistance identification and in-vivo inoculation determination of the control effect of a bactericide.

The invention discloses a negative pressure fixation and micro-injection combined cotton virus-induced gene silencing inoculation method. The method comprises the following steps: (1) constructing a virus-induced gene silencing vector; (2) preparing an agrobacterium suspension; (3) fixing under negative pressure; (4) performing micro-injection inoculation; and (5) subsequent culture: culturing the inoculated plant under the conditions that the temperature is 22-25 DEG C, the illumination period is 16h illumination / 8h darkness, the illumination intensity is 100-200 and the relative humidity is 60-80%, and observing the gene silencing phenotype after 7-14 days. The method solves the problems that a traditional virus-induced gene silencing inoculation method is complex in operation, low in success rate and large in leaf damage, has the advantages of being easy and convenient to operate, high in inoculation success rate, small in leaf damage, high in gene silencing efficiency and the like, and is suitable for cotton functional genomics research and gene function verification.

The invention discloses an inoculation device and an inoculation method for a tremella aurantialba liquid strain, and the inoculation method comprises the following steps: the middle end of a fungus bag is inoculated with a stereum hirsutum liquid strain, the inner surface of the fungus bag is inoculated with a tremella aurantialba liquid mixed strain, and the tremella aurantialba liquid mixed strain is a mixture of the tremella aurantialba liquid strain and the stereum hirsutum liquid strain. Preferably, the first inoculating needle is inserted into the middle end of the fungus bag to inoculate the stereum hirsutum liquid strain, and the second inoculating needle is inserted into the pit in the inner surface of the fungus bag to inoculate the tremella aurantialba liquid mixed strain. According to the method, the growth speed of tremella aurantialba hyphae is increased, the whole production period from inoculation to full fungus to fruiting is remarkably shortened, the yield is increased, and the production efficiency is improved.

The invention discloses a self-adaptive regulation and control system and a self-adaptive regulation and control method for accurate immersion inoculation of fishery vaccines. The system comprises a reaction tank, a plurality of control modules (temperature, dissolved oxygen, pH, salinity, concentration and pressure) and a controller. The controller adaptively adjusts vaccine liquid parameters based on sensor data, and the problems that an existing soaking inoculation method is unstable in immune effect and large in inoculation dosage fluctuation are solved.

The application discloses a chrysosporium strain ZJJE004 and a factory cultivation method thereof, the chrysosporium strain ZJJE004 ( Naematelia aurantialba ), is preserved in the China General Microbiological Culture Collection Center, and the preservation date is May 12, 2022, and the preservation number is CGMCC NO.40189, and the ITS sequence is shown as SEQ NO.1. The factory cultivation method of the chrysosporium strain ZJJE004 adopts a liquid strain one-time inoculation method, does not need to inoculate tissue blocks, greatly optimizes the cultivation method, and is simple in cultivation method, fast in mycelium growth, low in pollution, strong in mycelium, obviously shortens the cultivation period of the chrysosporium, and can be picked in 45-55 days; the ear yield is high, the size of the fruiting body is uniform, the mushroom effect is good, the conversion rate of the first crop can be higher than 80%, the yield is high, large-scale cultivation can be realized, has high application value, and is suitable for factory production.

The invention provides a rapid breeding method for rust-resistant oats. The method comprises the steps of rust-resistant parent material selection and hybridization, F1-generation population seed treatment, F2-generation population resistance identification and single plant selection, F3-F8-generation population screening, molecular marker-assisted selection and homozygous strain creation, and strain field identification and variety breeding. The invention aims to solve the technical problems that the anti-rust breeding of oat is difficult to combine with'temperature and light regulation and fertility stage suitability ', and the breeding period is too long. By controlling the temperature and red and blue light in a plant growth process in a staged precise control manner, the orientated shortening of the oat growth cycle, the improvement of the light energy utilization efficiency and the enhancement of the environmental stability in a key growth stage are realized, and a specific rust disease inoculation method is combined, so that the rust disease resistance phenotype can be stably expressed, and the precise screening of offspring is facilitated. By means of breeding screening of multiple breeding generations in the technology, an efficient and controllable rapid breeding system can be established, and rapid breeding of disease-resistant high-yield varieties is achieved.

This invention belongs to the technical field of bacterial culture transfer equipment, and more specifically, relates to an imprinted bacterial culture dot matrix inoculation block, device, and method. The invention includes a thin-film inoculation block body, which is divided into a smooth pickup section and an imprinted inoculation section. The imprinted inoculation section includes at least one pressure relief groove and dot matrix protrusions located on both sides of the pressure relief groove. Each dot matrix protrusion includes several raised portions, which are symmetrically and evenly distributed in a matrix on both sides of the pressure relief groove. Each raised portion has a concentric recess at its top center. This invention, through its thin-film inoculation block body, through-type pressure relief groove, matrix dot matrix protrusions, and concentric recess structure, combined with a porous liquid storage pad for quantitative liquid supply and an automated pickup and imprinting mechanism, solves the problems of poor consistency, vacuum adhesion, cross-contamination, low automation, and lack of process traceability in existing inoculation methods, achieving high-throughput, standardized, pollution-free, and traceable bacterial culture dot matrix inoculation.

The invention relates to the technical field of biological experiment equipment, and provides an automatic cell inoculation operation device and a liquid separation method for cell inoculation, and the automatic cell inoculation operation device comprises a liquid separation module, an inoculation needle assembly, a vibration module and a positioning module. A liquid storage cavity, a flow dividing pipe, a buffering groove, a thin liquid cavity and a liquid dividing cavity are sequentially formed in the liquid dividing module from top to bottom. The inoculating needle assembly is located above the liquid storage cavity and used for injecting cell suspension into the liquid storage cavity. The inlet of the overflow channel is higher than the highest liquid level of the buffer tank, the tail end of the overflow channel is connected to the bottom of the liquid storage cavity, and the highest position of the overflow channel is located above the liquid level of the liquid storage cavity. A liquid outlet channel is arranged below the liquid separation cavity, the liquid outlet channel is in one-to-one correspondence with the culture holes of the culture dish, the vibration module comprises a vibration pad, and the control system is electrically connected with the inoculation needle assembly, the liquid separation module, the vibration module and the positioning module. Cells are prevented from being accumulated in the center of the hole bottom due to the inertia effect, and the cells are evenly distributed in the hole.

The invention relates to a method for evaluating the soft rot resistance level of aseptically cultured colored callalily. The method comprises the following steps: preparing in-vitro leaves of aseptically cultured colored callalily to be detected; placing the in-vitro leaves on a moisturizing device for moisturizing culture; preparing a bacterial liquid of the Erwinia solani, wherein the concentration of the bacterial liquid is lower than or equal to the quorum sensing threshold concentration of the Erwinia solani; inoculating a predetermined volume of the bacterial liquid at a petiole incision of the in-vitro leaf; at different time points after inoculation, the morbidity condition of the in-vitro leaves is observed and quantified; and evaluating the soft rot resistance level of the aseptically cultured colored callalily based on the quantitative result of the morbidity condition. On the basis of sterile and rapid propagation of the zantedeschia aethiopica, the inoculation method has the characteristics of simplicity, convenience, high efficiency and easiness in popularization, is of great significance to development of disease-resistant breeding of the zantedeschia aethiopica, and provides a new technology and method for disease-resistant breeding of other plants, plant-microorganism interaction and research in related fields of biotechnology.

Methods of vaccination utilizing a cationic liposomal adjuvant admixed with at least one neoepitope and a solvent. Also provided are unit dosages and compositions for use in the methods.

The invention discloses brachybacterium paragglutinum and application of the brachybacterium paragglutinum to improvement of heat resistance of coral adults, the brachybacterium paragglutinum WH11-3 is preserved in Guangdong Microbial Culture Collection Center, and a method for improving the heat resistance of the coral adults by using the brachybacterium paragglutinum WH11-3 comprises the following steps: collecting the coral adults from seawater, and domesticating the coral adults in a water body; resuspending the pair of brevibacterium agglomerans into sterilized seawater to prepare a bacterial suspension; adding the bacterial suspension into a water body to infect coral adults, and transferring the coral adults into a fresh water body for continuous culture to obtain the coral adults infected with the brachybacterium paragasteroides. The photosynthetic efficiency of the coral adult in a high-temperature environment can be remarkably improved in a short time by inoculating the brachybacterium paragasteroides to the coral adult, the method can be popularized and applied to other coral adults except for the antler cup-shaped coral adult, and the inoculation method is easy to operate, low in cost, safe and free of pollution. The coral adult heat resistance is improved, so that the coral reef ecological system is assisted to be restored and protected.

The invention discloses a puncture type automatic uniform mixing peripheral blood inoculation instrument and method. The puncture type automatic uniform mixing peripheral blood inoculation instrument comprises a sample bearing platform, a puncture device, a cleaning device and a controller, the sample bearing platform comprises an annular uniform mixing frame capable of rotating along the circumference of a horizontal shaft, and the annular uniform mixing frame is provided with a plurality of assembly stations at equal angles in the circumferential direction and used for loading sample frames and sample pipes. When the annular uniform mixing frame rotates, the sample liquid is convectively stirred under the combined action of centrifugal force and gravity without an additional independent uniform mixing mechanism, so that the uniform mixing effect can be ensured, meanwhile, the equipment occupied space in an inoculation link in a biological test can be reduced, and further uniform mixing of the sample can be realized based on a sample group switching process; the problem of non-uniform sample recovery caused by long-time retention is avoided while the working efficiency is improved, and continuous switching processing of multiple samples can be realized.

To provide a safe and effective vaccine against all serotypes of dengue virus for subjects of a wide range of ages, and a corresponding inoculation method.SOLUTION: The present invention relates to unit doses of dengue vaccine compositions and methods and uses for preventing dengue disease and methods for stimulating an immune response against all four dengue virus serotypes in a subject or subject population. A unit dose of a dengue vaccine composition comprises constructs of each dengue serotype, such as TDV-1, TDV-2, TDV-3 and TDV-4, at various concentrations to improve protection against dengue infection.SELECTED DRAWING: Figure 1

Provided are improved methods for gene delivery to plants comprising injecting a polynucleotide, nucleoprotein complex, nanoparticle or plant viral nanoparticle into a stem or a petiole of the plant, thereby in it into the vascular system of the plant.

The invention provides a soil in-situ inoculation method of polymyxa graminis, belongs to the technical field of parasitic bacteria culture, and particularly provides a soil in-situ inoculation method of polymyxa graminis, which comprises the following steps: S1, selecting primary plants with parasitic characteristics of polymyxa graminis, and collecting diseased roots; s2, grinding the sick root into powder to obtain sick root powder, and detecting the content of polymyxa graminis in the sick root powder; s3, soil and the diseased root powder are mixed, culture medium soil is obtained, plant seedlings are transplanted into the culture medium soil, the plant seedlings are cultured, and soil in-situ inoculation of the polymyxin graminis is completed, or the plant seedlings are transplanted into the soil, the diseased root powder is smeared on the surface of the root system of the plant seedlings before transplanting, the plant seedlings are cultured, and soil in-situ inoculation of the polymyxin graminis is completed. The soil in-situ inoculation of the polymyxa graminis is completed. According to the method, soil in-situ inoculation and infection completion of polymyxa graminis are realized for the first time, and a foundation is laid for subsequent soil-borne virus infection and prevention and control research.

The application discloses a new rice variety breeding method for resisting rice smut and a misting inoculator, relates to the technical field of rice breeding, and comprises the following steps: (1) hybrid breeding of rice smut-resistant rice varieties; (2) single-plant selection of resistant varieties in a disease nursery; (3) rice smut resistance identification through misting inoculation; and (4) quality trait analysis to obtain a new rice variety resistant to rice smut and having high quality. The application screens rice varieties resistant to rice smut through multi-generation hybrid breeding and single-plant selection under natural disease infection conditions. The misting inoculation method is adopted to uniformly distribute rice smut chlamydospores in the air, and then the rice is contacted with the air, and the disease resistance is evaluated through moisture and heat preservation treatment. The application can efficiently obtain a new rice variety resistant to rice smut and having high quality, can realize improvement of inoculation controllability, cost saving, uniform inoculation, stable effect, enhancement of the anti-infection capacity of the new variety, and can breed a new rice variety with early maturity and high yield.

The invention belongs to the technical field of gene breeding, and particularly relates to application of cascade signal kinase OsMAPKKK5 in improvement of bacterial blight resistance of rice. The invention provides a protein OsMAPKKK5 or a substance for regulating and controlling the activity and / or content of the protein, and application of a biological material related to the protein OsMAPKKK5. According to the invention, an OsMAPKKK5 overexpression transgenic line is constructed, Xanthomonas oryzae pv. Oryzae is inoculated through a field inoculation method for resistance phenotype analysis, and the OsMAPKKK5 overexpression transgenic line is found to be shorter in scabs and show a Xanthomonas oryzae resistance phenotype compared with a wild type rice variety, so that it is proved that the Xanthomonas oryzae resistance character of rice can be promoted by increasing the expression quantity of OsMAPKKK5. The invention provides an important gene resource and a molecular theoretical basis for developing a new variety of bacterial blight resistant rice.

The invention belongs to the technical field of edible fungus cultivation, and particularly relates to a pilose antler fungus liquid strain inoculation method and a strain and culture medium mixing device, in particular to an inoculation method for achieving three-dimensional uniform distribution of mycelium pellets through sterile mechanical mixing of a liquid strain and a culture medium. And the special mixing device consists of a conical mixing barrel, a mounting cover and a double-helix stirring part. Compared with the prior art, by arranging the conical material mixing barrel with the spiral water channel and the double-spiral stirring component, the technical problems of asynchronous mycelial growth, long spawn running period and high infectious microbe pollution risk caused by a traditional point connection mode are solved, rapid and synchronous growth of mycelia in a culture medium is achieved, and the yield of the mycelia is improved. The spawn running period is shortened by more than 30%, and the production success rate and the strain quality are remarkably improved.