58 results about "Rhizobium japonicum" patented technology

The invention relates to a complex microbial inoculum 707 and a preparation method and application thereof, and belongs to the technical field of biological materials of the application of plant biological regulators to crop planting. The preparation method of the complex microbial inoculum 707 comprises the following step of: performing the cultivation of one-level strains and second-level and third-level fermentation on strains comprising 45 to 55 percent of bacillus subtilis, 5 to 10 percent of bacillus licheniformis, 5 to 10 percent of bacillus megaterium, 5 to 10 percent of candida utilis, 5 to 10 percent of rhizobium japonicum, 5 to 10 percent of bacillus mucilaginosus and 5 to 10 percent of lactobacillus plantarum, so that the microbial content of fermentation liquor reaches 6-10*10<9> per milliliter to obtain the complex microbial inoculum 707. Through long-term field verification, the complex microbial inoculum 707 has the advantages of reasonable formula and stable effect, can promote the growth of crops, improve fruit quality, promote early ripening, increase yield, improve the utilization rate of fertilizers and improve the ecological environment of soil, and has certain effect on disease prevention and disease resistance.

The invention relates to the field of microbiology, and specifically relates to a composite microbial fertilizer and a preparation method thereof. The composite microbial fertilizer is composed of a composite microbial fermentation broth and an embedding medium, wherein a volume ratio of the composite microbial fermentation broth to the embedding medium are in a range of 1:5 to 1:4. The compositemicrobial fermentation broth is compounded of a co-culture comprising Candida utilis, lactic streptococci, lactobacillus acidophilus and bacillus subtilis, and a single nutrient solution comprising Bradyrhizobium japonicum, Azospirillum lipoferum, Rhodopseudomonas palustris, Bacillus megaterium, Bacillus mucilaginosus and Azotobacter chrococcum. In the invention, composite microbes processed by embedding can be stored stably for more than 60 days and a count of the composite microbes is always more than 2 billion per gram, thus the composite microbial fertilizer comprising the composite microbes has a long effective time and can improve a planting capacity of soil.

The invention discloses an Azorhizobium strain SCAUs152 and application thereof. The strain is a new strain which is separated and purified from fresh soybean root nodules and belongs to Rhizobium. The strain is collected to China Center for Type Culture Collection of Wuhan University on March 14th, 2014, and the collection number is CCTCC NO.M2014085. The strain is applied to production of Sichuan soybeans. The Azorhizobium strain SCAUs152 is a favorable broad-spectrum Rhizobium japonicum strain with high symbiotic fixation capacity, wide application range of Sichuan soybean species, IAA (indoleacetic acid) secretion capacity, capacities for dissolving inorganic phosphorus, organic phosphorus and potassium and high stress tolerance. The strain has favorable matching affinity with Sichuan dominant bean species, and can enhance the soybean yield by more than 23% in different ecological regions where no nitrogen fertilizer is applied and the Azorhizobium strain SCAUs152 is inoculated, which is much different from the regions where the Azorhizobium strain SCAUs152 is not inoculated.

The invention relates to bradyrhizobium japonicum capable of effectively fixing nitrogen and a culture method and application thereof. The bradyrhizobium japonicum CGMCC No.4346 is cultured by the steps of strain activation, seed culture, fermentation culture and the like. The invention has the following beneficial effects: sorbitol and diethylene glycol are utilized to replace the carbon source, mannitol in the traditional YEM (yeast extract mannitol) culture medium, and when the improved fermentation culture medium is adopted for culturing, the viable count of the fermentation liquor is higher than that of the YEM culture medium; compared with the rhizobium inoculants which are not inoculated on the soy beans, the bradyrhizobium japonicum agent ensures the theoretical yield of the soy beans to be increased by 16.7-17.2%, the crude fat content to be increased by 0.65-1.21% and the protein content to be increased by 1.29-1.60% after being inoculated on the soy beans, thus being an excellent strain for production of rhizobium japonicum and inoculation of the soy beans; and the culture method is simple and practical, and the raw materials have wide sources and low price and are suitable for large-scale industrialized production.

The invention discloses an organic fertilizer containing medical dregs and biological germs and a preparation method thereof. The organic fertilizer containing medical dregs and biological germs, provided by the invention, comprises the following components in parts by weight: 15 parts of Chinese prickly meal, 10 parts of bean meal, 15 parts of plant ash, 20 parts of medicine residue, 15 parts of corm pulp, 10 parts of bone meal, 10 parts of bentonite and 5 parts of biological germ; and the biological germ is either any one of or the mixture of more than one of bacillus megatherium, rhizobium japonicum, basil rhizobia and EM flora. According to the organic fertilizer provided by the invention, the Chinese prickly meal and the medicine residue are added in the active ingredients, the Chinese prickly meal has an insecticidal property, and the medicine dregs has medical value, so that the synergistic effect of the two can reduce crop diseases and insect pests, reduce the usage of pesticide, and preserve the ecological environment. After using the organic fertilizer containing medical dregs and biological germs, the applicant found that the production of sweet potato increases more than 24 percent a year, the nutrient substance in the organic fertilizer is provided to the plants, the production cost is low, and the method can be widely used in agricultural field.

The invention discloses a preparation method of a bacterial fertilizer or a bacterial product. The preparation method is characterized by comprising the following steps: uniformly mixing a type A microbial agent and a type B microbial agent in the ratio of 1 to (0.1 to 10); adding the uniformly-mixed microbial agent into a pesticide residue culture medium to obtain the bacterial fertilizer or thebacterial product. Active components of the microbial agent consist of rhizobium japonicum, bacillus megaterium, bacillus cereus, bacillus subtilis, bacillus mucilaginosus, lactobacillus buchneri, bacillus amyloliquefaciens, paenibacillus polymyxa, bacillus pumillus, bacillus licheniformis, trichoderma harzianum, etc. The microbial agent and pesticide residues are mixed uniformly to obtain the bacterial fertilizer or the bacterial product. The bacterial fertilizer or the bacterial product can be applied to the facilitation of crop growth or the improvement of crop yield or the improvement of planting soil, as well as the improvement of crop disease resistance. The product can be combined with different bacterial fertilizer carriers to form a solid bacterial fertilizer, a dry or gel starchbacterial fertilizer, a liquid bacterial fertilizer, a pesticide residue waste bacterial fertilizer, a grass charcoal bacterial fertilizer, a peat bacterial fertilizer, a hibernated bacterial fertilizer, as well as a pig, cattle, sheep and chicken manure excrement bacterial fertilizer, and to form seed coatings.

The invention discloses a mutant of rhizobium japonicum SMH12 and application of the mutant. The nopP gene deletion mutant is constructed by utilizing a CreloxP system double-exchange replacement method. By taking fast-growing rhizobium SMH12 genome DNA as a template and adopting a nopP-up-F/R primer pair and a nopP-down-F/R primer pair, a nopP homologous exchange upper arm and a nopP homologous exchange lower arm are respectively amplified, a product is connected with a digested pCM351 vector in two steps after being subjected to double enzyme digestion, plasmid transformation is obtained, and the mutant can be obtained. Therefore, the rhizobium target gene mutant is constructed and obtained. After the nopP gene is mutated or deleted, the defense reaction of leguminous plants is inhibitedor eliminated, so that rhizobium infection and nitrogen fixation are promoted. The improved rhizobium can improve the growth and nitrogen fixation capacity of leguminous crops, reduce nitrogen fertilizer application in agricultural production and generate huge economic and ecological benefits.

The invention relates to a constructing method of a nifA gene-introduced rhizobium japonicum genetic engineering bacterial strain HD-SFH-01, belonging to a constructing method of a rhizobium japonicum genetic engineering bacterial strain and solving the problems of less increase on soybean nodule quantity and little soybean yield increase after the traditional rhizobium japonicum genetic engineering bacterial strain is inoculated to a soybean seedling. The method comprises the steps of: extracting rhizobium japonicum genome DNA for carrying out PCR amplification to obtain a target gene nifA, purifying and then connecting with a TA clone carrier pMD18-T to obtain a connecting product and then converting into escherichia coli; carrying out multiple enzyme digestion, connection and verification to obtain a recombinant prokaryote expression carrier pTR-Plac-nifA; and converting the recombinant prokaryote expression carrier pTR-Plac-nifA into soybean indigenous rhizobium. The bacterial strain HD-SFH-01 is inoculated into the soybean seedling, the rhizobium quantity is increased, and the soybean yield is increased by 3.80 percent.

The invention discloses an organic fertilizer containing pepper meal and biological germs and a preparation method thereof. The organic fertilizer containing pepper meal and biological germs, provided by the invention, comprises the following components in parts by weight: 15 parts of pepper meal, 15 parts of humic acid, 10 parts of amino acid, 5 parts of cigarette foam, 30 parts of bacterial sludge, 10 parts of starch dregs, 10 parts of bentonite and 5 parts of biological germ; and the biological germ is either any one of or the mixture of more than one of bacillus megatherium, rhizobium japonicum, basil rhizobia and EM flora. The active ingredient pepper meal of the organic fertilizer, provided by the invention, comprises capsorubin, carotene, zeaxanthine, VC and capsaicin, the mixed use of the pepper dregs with humic acid and bacterizl sludge can improve the physicochemical property of the soil, promote the microorganism in the soil to convert the organic fertilizer into components that the crop can absorb, and remarkably improve the manufacturing efficiency of the crop. After using the organic fertilizer containing pepper meal and biological germs, the applicant found that the production of potato increases more than 11 percent a year, and the pollution of the organic fertilizer to the environment is decreased.

The invention discloses an organic fertilizer containing furfural residues and trace elements and a preparation method thereof. The organic fertilizer comprises the following components in parts by weight: 15 parts of furfural residues, 13 parts of chicken manure, 5 parts of amino acid, 15 parts of humic acid, 12 parts of bone meal, 20 parts of lignin, 15 parts of bentonite, 4.5 parts of biological bacterium and 0.5 part of trace element fertilizer, wherein the furfural residues are furfural waste residues generated in the furfural production process; and the biological bacterium is any one of or the mixture of more than any two of bacillus megaterium, rhizobium japonicum, rhizobium astragali and EM (effective microorganisms). The organic fertilizer and the preparation method have the advantages that the contents of CO3<2-> and HCO3<-> in soil can be reduced by adding lots of furfural residues to the active components, thus effectively improving the physical and chemical properties of soil and reducing the alkalinity of soil; and as the content of the furfural residues in the organic fertilizer is higher, Cl<-> can be reduced and the contents of such elements as organic matters, potassium and nitrogen in salinity soil can be increased by applying the organic fertilizer so that the elements promote growth of crops together with other nutrients in the organic fertilizer.

The invention discloses a preparation method of a soybean root exudate for efficiently promoting competitive nodulation of rhizobium japonicum, and belongs to the technical field of bioengineering. The method comprises the following steps: (1) sterilizing the surface of a soybean seed; (2) moving the sterilized soybean seed into a YMA agar watch glass under the aseptic condition to germinate, moving to a nitrogen-free Rigaud-Puppo nutrient solution of which the concentration is 30%, to culture after the seed is germinated, and collecting the soybean root exudate on the 12 day of culturing, so as to obtain the soybean root exudate for efficiently promoting competitive nodulation of the rhizobium japonicum. The preparation method has the advantages: the best concentration of the nitrogen-free Rigaud-Puppo nutrient solution is confirmed to be 30%, the soybean root exudate collected on the 12 day of culturing remarkably improves the expression of a nodulation gene of the rhizobium japonicum, and the effect of efficiently promoting competitive nodulation of the soybean is further reflected.

The invention discloses preparation and application of a novel alfalfa rhizobia immobilized embedded granule formation, and belongs to the field of agricultural microbe application. An immobilized cell technology is a method for immobilizing microbial cells in some special materials, preparing granules to form a limited space, enabling the thallus to keep biological activity in a certain time and realizing proliferation. At present, the alfalfa rhizobia is widely applied to the fields such as medicines, foods, fermentation industry, wastewater treatment and the like, preliminary study in a rhizobium japonicum embedding aspect is realized, while the preparation and application of the alfalfa rhizobia embedded immobilized granules is still a blank at present. According to the novel alfalfa rhizobia immobilized embedded granules developed in the invention, the alfalfa rhizobia is conveniently added by alfalfa cultivation enterprises, and the activity of fungicides is not lost in the compounding, processing, granulating and transportation and storage processes. Therefore, alfalfa rhizobia bacterial manure can be easily used by farmer households, and the main technical bottleneck difficulty of a high-tolerance high-activity alfalfa rhizobia seed embedding technique is broken.

The invention discloses a seed coating agent for promoting germination and seedling formation of leguminous seeds in saline-alkali soil and a preparation method of the seed coating agent. The seed coating agent mainly comprises a soybean rhizobium solution, a bacillus liquid a, a bacillus liquid b, silk fibroin, peptone and the like. A film is formed outside the seeds before sowing, so that the seeds are helped to resist abiotic stress outside soil, and a protective layer can be formed around rhizosphere during seedling growth, so that the harm of abiotic stress to the seedlings is further reduced, and the leguminous seeds can grow healthily.

The invention relates to an environment-friendly biological agent for treating organic garbage and a preparation method of the environment-friendly biological agent. The environment-friendly biological agent, in parts by weight, comprises 8-13 parts of bacillus licheniformis powder, 10-15 parts of bacillus pumilus powder, 13-16 parts of nitrobacteria powder, 8-10 parts of lactic acid bacteria powder, 7-10 parts of bacillus megaterium powder, 8-12 parts of bacillus subtilis powder, 9-14 parts of bacillus amyloliquefaciens powder, 5-8 parts of saccharomyces cerevisiae powder, 1-5 parts of bacillus mucilaginosus powder, 1-5 parts of rhizobium japonicum powder, 2-4 parts of azotobacter chroococcum powder, 3-7 parts of thermomonospora fusca powder, 1-3 parts of lactobacillus delbrueckii, 1-3 parts of cellulase, 0.5-2 parts of ligninase, 0.01-0.05 part of 90% amino-oligosaccharin and 0.05-1 part of brassinolide. The biological agent can be applied to treatment of different organic solid wastes, has low requirements on working conditions, can start self-fermentation at normal temperature, does not need high-temperature and high-pressure catalysis, greatly shortens the treatment period to24 hours to one week, and is environment-friendly and harmless; and a final treatment product is biological bacterial fertilizer, so that waste is turned into wealth.

The invention belongs to the technical field of biology, and relates to application of a new gene, in particular to an R gene for controlling soybean-rhizobium matching property as well as protein and application of the R gene. The GmNNL1 genome sequence of the gene GmNNL1 disclosed by the invention in the HENGFENG WUDOU of a soybean line is as shown in SEQ ID NO. 2 and the coded amino acid sequence is as shown in SEQ ID NO. 3. The R gene GmNNL1 of the soybean is an effective gene capable of regulating and controlling the nodulation quantity of specific rhizobium on the soybean, and can regulate and control the nodulation quantity by directly identifying the haplotype of slow-growing rhizobium specific effect protein NopP to limit the symbiotic nodulation of indigenous rhizobium, so that the soybean preferentially nodulates with artificially applied efficient rhizobium inoculant; and the symbiotic nitrogen fixation capability can be improved.

The invention discloses a nitrogen-fixing bradyrhizobium japonicum strain resistant to saline-alkali soil and application of the bradyrhizobium japonicum strain. According to the invention, a strain of bradyrhizobium taihuae Dian strain is obtained through separation and is preserved in Guangdong Microbial Culture Collection Center on January 11, 2022, and the preservation number is GDMCC No: 62202. Researches show that the strain has the characteristics of high nodulation rate and strong nitrogen fixation capability, and can optimize the micro-ecological environment of alkaline soil crop root growth; the Dian strain is adopted to treat plants, soybean nodulation and nitrogen fixation can be effectively promoted, plant nitrogen nutrition is increased, plant growth is promoted, and the biomass, root nodule number and nitrogen content of soybean plants can be remarkably improved. The strain is especially suitable for cultivation and production of soybeans in stony desertification areas, expands a strain resource library of nitrogen-fixing rhizobium, is beneficial to promoting nodulation and nitrogen fixation of plants, and increases nitrogen nutrition of the plants.