58 results about "Rhizobium japonicum" patented technology

The invention discloses an organic fertilizer containing furfural slag and biological bacteria, as well as a preparation method thereof. The organic fertilizer containing furfural slag and biological bacteria comprises components in parts by weight as follows: 15 parts of furfural slag, 13 parts of chicken manure, 5 parts of amino acid, 15 parts of humic acid, 12 parts of bone powder, 20 parts of xylogen, 15 parts of bentonite and 5 parts of biological bacteria; the furfural slag is waste furfural slag generated in a furfural slag production process; and the biological bacteria are a mixture of one or more than two of bacillus megatherium, rhizobium japonicum, purple perilla rhizobium and an EM (effective microorganism) flora. According to the organic fertilizer containing furfural slag and biological bacteria, as well as the preparation method thereof, a large amount of furfural slag is added in the effective components, so that contents of CO3<2->and HCO3<-> in the soil can be reduced, physical and chemical properties of soil are effectively improved, and alkalinity of soil is reduced; and further, the content of the furfural slag in the organic fertilizer is higher, during application, Cl<-> can be further reduced, contents of elements such as organic matters, potassium, nitrogen and the like in salinity soil are increased, and the elements and other nutritional ingredients in the organic fertilizer are combined to promote growth of crops.

The invention relates a rhizobium japonicum culture medium and a method for preparing a liquid rhizobium japonicum agent by adopting the rhizobium japonicum culture medium and aims at solving the problem that an existing liquid rhizobium japonicum agent is unbalanced in nutrition and low in thallus activity and rhizobium japonicum prepared by means of a fermentation technology cannot stably survive at the room temperature. The rhizobium japonicum culture medium is an aqueous solution prepared by glycerin, mannitol, glucose, rhamnose, fructose, alpha-oxoglutarate, yeast powder, serine, arginine, NH4C1, K2HPO4, KH2PO4, MgSO4*7H2O, CaCl2*2H2O, FeSO4*7H2O, NaCl, Na2MoO4*2H2O and biotin, and can be obtained by controlling fermentation tank conditions. The viable count of microbial inoculum of the rhizobium japonicum culture medium preserved at 20 DEG C for 12 months can still reach to more than 100 hundred millions per milliliter.

The invention relates to a complex microbial inoculum 707 and a preparation method and application thereof, and belongs to the technical field of biological materials of the application of plant biological regulators to crop planting. The preparation method of the complex microbial inoculum 707 comprises the following step of: performing the cultivation of one-level strains and second-level and third-level fermentation on strains comprising 45 to 55 percent of bacillus subtilis, 5 to 10 percent of bacillus licheniformis, 5 to 10 percent of bacillus megaterium, 5 to 10 percent of candida utilis, 5 to 10 percent of rhizobium japonicum, 5 to 10 percent of bacillus mucilaginosus and 5 to 10 percent of lactobacillus plantarum, so that the microbial content of fermentation liquor reaches 6-10*10<9> per milliliter to obtain the complex microbial inoculum 707. Through long-term field verification, the complex microbial inoculum 707 has the advantages of reasonable formula and stable effect, can promote the growth of crops, improve fruit quality, promote early ripening, increase yield, improve the utilization rate of fertilizers and improve the ecological environment of soil, and has certain effect on disease prevention and disease resistance.

The invention relates to a construction method of genetic engineering strains for improving quantity of soybean nodules. The construction method aims at solving the problems that when the conventional rhizobium japonicum genetic engineering strains are inoculated to soybean seedlings, the root nodule quantity of soybean plants and the yield increase of soybeans are small compared with those of the original starting strains. The construction method comprises the following steps of: performing activation and culture expansion on sinorhizobium fredii to extract genomic DNA (deoxyribonucleic acid); performing PCR (Polymerase Chain Reaction) amplification by virtue of a homologous sequence cloning method to obtain target gene dct A; performing sequencing identification on the target gene; constructing a prokaryotes expression vector pTR-Plac-dct A; and converting the prokaryotes expression vector into the sinorhizobium fredii to obtain the gene engineering strains. The quantity of the root nodules on the soybean plant with the transformed gene engineering strains is increased by 53.73%, and the yield of the soybeans is increased by 62.02%.

The invention discloses an Azorhizobium strain SCAUs152 and application thereof. The strain is a new strain which is separated and purified from fresh soybean root nodules and belongs to Rhizobium. The strain is collected to China Center for Type Culture Collection of Wuhan University on March 14th, 2014, and the collection number is CCTCC NO.M2014085. The strain is applied to production of Sichuan soybeans. The Azorhizobium strain SCAUs152 is a favorable broad-spectrum Rhizobium japonicum strain with high symbiotic fixation capacity, wide application range of Sichuan soybean species, IAA (indoleacetic acid) secretion capacity, capacities for dissolving inorganic phosphorus, organic phosphorus and potassium and high stress tolerance. The strain has favorable matching affinity with Sichuan dominant bean species, and can enhance the soybean yield by more than 23% in different ecological regions where no nitrogen fertilizer is applied and the Azorhizobium strain SCAUs152 is inoculated, which is much different from the regions where the Azorhizobium strain SCAUs152 is not inoculated.